##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211363_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9121319 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08409540330735 33.0 31.0 34.0 30.0 34.0 2 32.21329897572928 33.0 31.0 34.0 30.0 34.0 3 32.2831024767361 34.0 31.0 34.0 30.0 34.0 4 35.926448795399 37.0 35.0 37.0 35.0 37.0 5 35.829468852037735 37.0 35.0 37.0 35.0 37.0 6 35.84333778919474 37.0 35.0 37.0 35.0 37.0 7 35.75748671875197 37.0 35.0 37.0 33.0 37.0 8 35.73995296075052 37.0 35.0 37.0 33.0 37.0 9 37.355787030362606 39.0 37.0 39.0 34.0 39.0 10-11 37.30263605515825 39.0 37.0 39.0 34.0 39.0 12-13 37.232798677471976 39.0 37.0 39.0 33.0 39.0 14-15 38.46593080452509 40.0 38.0 41.0 34.0 41.0 16-17 38.44582548861628 40.0 38.0 41.0 34.0 41.0 18-19 38.44385565289406 40.0 38.0 41.0 34.0 41.0 20-21 38.409266905367524 40.0 38.0 41.0 34.0 41.0 22-23 38.295690239536626 40.0 38.0 41.0 34.0 41.0 24-25 38.20523610675167 40.0 38.0 41.0 33.0 41.0 26-27 38.108759270452005 40.0 37.5 41.0 33.0 41.0 28-29 37.99917446150058 40.0 37.0 41.0 33.0 41.0 30-31 38.01428439242176 40.0 37.0 41.0 33.0 41.0 32-33 37.96010072666026 40.0 37.0 41.0 33.0 41.0 34-35 37.828675764985306 40.0 37.0 41.0 33.0 41.0 36-37 37.68040713190713 40.0 37.0 41.0 33.0 41.0 38-39 37.56557812526894 40.0 37.0 41.0 32.0 41.0 40-41 37.457724151518 40.0 36.5 41.0 32.0 41.0 42-43 37.35572941808087 40.0 36.0 41.0 31.5 41.0 44-45 37.20613405802385 39.5 36.0 41.0 31.0 41.0 46-47 36.976581731216726 39.0 36.0 41.0 31.0 41.0 48-49 35.43052956485789 39.0 35.0 40.5 24.0 41.0 50-51 36.16988420205455 39.0 35.0 40.0 29.0 41.0 52-53 36.268700009285936 39.0 35.0 40.0 29.5 41.0 54-55 36.09579524627962 38.0 35.0 40.0 29.0 41.0 56-57 35.8793357079168 38.0 35.0 40.0 28.5 41.0 58-59 35.64052835998829 38.0 34.0 40.0 28.0 41.0 60-61 35.380770094763704 37.5 34.0 40.0 28.0 41.0 62-63 35.04967302426327 37.0 34.0 40.0 27.5 41.0 64-65 34.7114933706408 36.5 33.5 39.0 27.0 41.0 66-67 34.369633547516536 36.0 33.0 39.0 26.0 40.5 68-69 34.001928449163984 35.5 33.0 39.0 26.0 40.0 70-71 33.635719132287775 35.0 32.5 38.0 26.0 40.0 72-73 33.25390752148894 35.0 32.0 37.0 26.0 39.5 74-75 32.887861503363716 35.0 32.0 37.0 25.0 39.0 76-77 31.954465357477353 34.0 30.5 36.0 24.5 38.0 78-79 32.39007631462073 34.5 31.5 36.0 25.0 38.0 80-81 32.262394945292456 34.5 31.5 35.5 25.0 37.0 82-83 31.99252142151809 34.0 31.0 35.0 24.5 37.0 84-85 31.70994946016031 34.0 31.0 35.0 24.0 36.0 86-87 31.454555530839343 34.0 31.0 35.0 24.0 36.0 88-89 31.227576241988686 34.0 31.0 35.0 23.0 35.5 90-91 31.009960894910044 34.0 30.5 35.0 23.0 35.0 92-93 30.816599660641184 34.0 30.0 35.0 20.5 35.0 94-95 30.6191177504043 34.0 30.0 35.0 20.0 35.0 96-97 30.427447499643417 34.0 29.5 35.0 20.0 35.0 98-99 30.20469095533223 34.0 29.0 35.0 19.0 35.0 100 27.740866973296296 31.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.31997515069616256 1101 2 -0.2618122097762061 1101 3 -0.34122766788373937 1101 4 -0.18235383821558315 1101 5 -0.20196074218891624 1101 6 -0.25648581597019415 1101 7 -0.23099793539190472 1101 8 -0.2682284131553345 1101 9 -0.31736932296919207 1101 10-11 -0.3485282597818369 1101 12-13 -0.4580016985809081 1101 14-15 -0.4969182444500504 1101 16-17 -0.48283347606857774 1101 18-19 -0.5272961737488728 1101 20-21 -0.574088104107453 1101 22-23 -0.5912331388263041 1101 24-25 -0.6034202255728474 1101 26-27 -0.6344087195400974 1101 28-29 -0.7030345406546843 1101 30-31 -0.679802974505705 1101 32-33 -0.6580177557560063 1101 34-35 -0.6926507270685889 1101 36-37 -0.6345905223370423 1101 38-39 -0.6488225646998202 1101 40-41 -0.6503945892617438 1101 42-43 -0.649804381298253 1101 44-45 -0.6881882190904278 1101 46-47 -0.6948702467276746 1101 48-49 0.5457420496814578 1101 50-51 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68-69 -0.911213505393917 1105 70-71 -0.8761061821084795 1105 72-73 -0.8001904617563937 1105 74-75 -0.9219584134574816 1105 76-77 -0.8678601420529759 1105 78-79 -0.7527034550730463 1105 80-81 -0.7346018769759404 1105 82-83 -0.7287539851040314 1105 84-85 -0.7899980092327432 1105 86-87 -0.7256956014330669 1105 88-89 -0.7083491476113757 1105 90-91 -0.7738797049727175 1105 92-93 -0.6929093083764606 1105 94-95 -0.7386325289148878 1105 96-97 -0.7685290071690645 1105 98-99 -0.7553711786397947 1105 100 -0.8663914234973724 1106 1 -0.33847919933525716 1106 2 -0.278840754390675 1106 3 -0.31166665748826006 1106 4 -0.18761547655194022 1106 5 -0.26708210160054335 1106 6 -0.36284982954509104 1106 7 -0.3104363024130947 1106 8 -0.3772642957549621 1106 9 -0.491073132181576 1106 10-11 -0.4307555932223224 1106 12-13 -0.47401925653934995 1106 14-15 -0.6589334044465716 1106 16-17 -0.5898880066093426 1106 18-19 -0.6467381100015146 1106 20-21 -0.6287607194932079 1106 22-23 -0.6761974077157973 1106 24-25 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0.34881590740133817 2311 6 0.3790991294343158 2311 7 0.3902743115016989 2311 8 0.21269092243544208 2311 9 0.45433557722601847 2311 10-11 0.43144409267909367 2311 12-13 0.5368003814506466 2311 14-15 0.4791068878016773 2311 16-17 0.5269445253553187 2311 18-19 0.6425949596862708 2311 20-21 0.715006659775149 2311 22-23 0.7169248176044221 2311 24-25 0.7112817332622754 2311 26-27 0.7953969341417988 2311 28-29 0.8046593544151435 2311 30-31 0.645637772951126 2311 32-33 0.7028066544229858 2311 34-35 0.8116011995668657 2311 36-37 0.8882656508124356 2311 38-39 0.9215724543781292 2311 40-41 0.831657662621673 2311 42-43 0.8477251376529225 2311 44-45 0.8715340764719315 2311 46-47 0.9462136749744161 2311 48-49 -6.0263195088878305 2311 50-51 -1.0090516967760053 2311 52-53 0.47146785350008713 2311 54-55 0.8245106984320003 2311 56-57 0.977713416352529 2311 58-59 0.9815280920879346 2311 60-61 0.8244710885547448 2311 62-63 0.6899151897676674 2311 64-65 0.7818358436057053 2311 66-67 0.9461022361903346 2311 68-69 0.9806111907643071 2311 70-71 1.0297491519619726 2311 72-73 0.502904236845886 2311 74-75 0.7442716106244802 2311 76-77 0.8726539494804335 2311 78-79 0.7985580215441814 2311 80-81 0.8417318738829564 2311 82-83 0.8228640834655359 2311 84-85 0.863139528882396 2311 86-87 0.8911992377387961 2311 88-89 0.9104012548522853 2311 90-91 1.0159218932470786 2311 92-93 1.059264224076383 2311 94-95 1.0426755996437116 2311 96-97 1.0258380153608506 2311 98-99 1.0247915520542037 2311 100 1.2890271460296887 2312 1 0.13011986073744453 2312 2 0.2988464641389186 2312 3 0.25009642760247175 2312 4 0.015050443028947313 2312 5 0.19602262815284632 2312 6 0.14462227030291785 2312 7 0.28442455052540794 2312 8 0.3524305430496071 2312 9 0.2570374458527027 2312 10-11 0.21959719469169414 2312 12-13 0.28103504492376885 2312 14-15 0.36547884682639165 2312 16-17 0.5262669203046642 2312 18-19 0.40134019392699116 2312 20-21 0.40103999070520757 2312 22-23 0.674034044128689 2312 24-25 0.7500713596639628 2312 26-27 0.6924964158053726 2312 28-29 0.7752169225330121 2312 30-31 0.8045683286085534 2312 32-33 0.8090292005992623 2312 34-35 0.37091349900513393 2312 36-37 0.6747017556017951 2312 38-39 0.8479822747002217 2312 40-41 0.5831075080760897 2312 42-43 0.5931613601606358 2312 44-45 0.7177140311485815 2312 46-47 0.7431097570466463 2312 48-49 -4.711206848136541 2312 50-51 -0.8451689378086158 2312 52-53 0.503533101129932 2312 54-55 0.7873348152357877 2312 56-57 0.8504627059723973 2312 58-59 0.8952799967367824 2312 60-61 0.9272969416146992 2312 62-63 0.7298174423923172 2312 64-65 0.9373785484405488 2312 66-67 0.9462979429414133 2312 68-69 0.8649634750816659 2312 70-71 0.8809406253251524 2312 72-73 0.9949449738342935 2312 74-75 0.6979403977122445 2312 76-77 0.8864459731858325 2312 78-79 0.549944065622725 2312 80-81 0.7406893240677874 2312 82-83 0.7329594022291026 2312 84-85 0.7984833398045517 2312 86-87 0.9043219351419935 2312 88-89 0.8949803303625181 2312 90-91 0.7240762189580003 2312 92-93 0.8961697039369731 2312 94-95 1.0773749831251784 2312 96-97 1.0913859298586885 2312 98-99 1.14130009209088 2312 100 0.6784435957910588 2313 1 0.018415415129510393 2313 2 0.21116388487590143 2313 3 0.26556975687907425 2313 4 0.08801100159146813 2313 5 0.1417677367448178 2313 6 0.2576078080827884 2313 7 0.158474344648333 2313 8 0.23885516259128536 2313 9 0.4735217015134481 2313 10-11 0.20196200388118513 2313 12-13 0.027368969245671337 2313 14-15 0.11952516324906526 2313 16-17 0.06544469745195869 2313 18-19 0.4009427604976281 2313 20-21 0.4120425213500525 2313 22-23 0.48069871810908893 2313 24-25 0.2344870459719175 2313 26-27 0.34369544347659087 2313 28-29 0.33506223449560224 2313 30-31 0.518010488605043 2313 32-33 0.4274336063128601 2313 34-35 0.36207872179377887 2313 36-37 -0.35455164760116986 2313 38-39 0.13600073575345561 2313 40-41 0.3792618991687178 2313 42-43 0.5247192880639773 2313 44-45 0.4541933705108576 2313 46-47 0.3375776158206918 2313 48-49 -5.22649056616363 2313 50-51 -1.2996888298014824 2313 52-53 0.07590246370472897 2313 54-55 0.2694007432421799 2313 56-57 0.34355616385775534 2313 58-59 0.42475871940776955 2313 60-61 0.70920515469642 2313 62-63 0.7686891758257204 2313 64-65 0.8673623426706953 2313 66-67 0.8805958478271805 2313 68-69 0.9051895186354244 2313 70-71 0.9144596202175705 2313 72-73 0.6372507143372843 2313 74-75 0.7618234885000632 2313 76-77 0.783879550503567 2313 78-79 0.7629590106827209 2313 80-81 -0.14524391366069977 2313 82-83 0.3054272430750551 2313 84-85 0.6468712133875307 2313 86-87 0.7885071237245569 2313 88-89 0.8060558570304615 2313 90-91 0.821988667593164 2313 92-93 0.8587282922204302 2313 94-95 0.789069259331864 2313 96-97 0.7983950667415272 2313 98-99 0.9935059165947777 2313 100 0.9926734383581106 2314 1 0.07048751638193096 2314 2 0.20010704183944483 2314 3 0.22864334181101498 2314 4 0.053418508452615754 2314 5 0.21902763142446702 2314 6 0.28676211075341485 2314 7 0.1142882438695807 2314 8 0.2662617360578068 2314 9 0.4400313454303628 2314 10-11 0.3194966666052963 2314 12-13 0.21843845490343483 2314 14-15 0.2490347827862749 2314 16-17 0.4692785549490708 2314 18-19 0.5432617498541461 2314 20-21 0.5801754447559659 2314 22-23 0.3261064252321404 2314 24-25 0.45053888246067686 2314 26-27 0.5418579534026193 2314 28-29 0.6593550313359913 2314 30-31 0.5758630105119593 2314 32-33 0.5421759301868576 2314 34-35 0.4536975519856128 2314 36-37 0.5390616518792015 2314 38-39 0.5433537086850109 2314 40-41 0.3893850167067896 2314 42-43 0.4502395685003009 2314 44-45 0.6042187689449818 2314 46-47 0.6838389436790351 2314 48-49 -6.455924904637598 2314 50-51 -1.4948499709809013 2314 52-53 -0.7342105797886731 2314 54-55 -0.27195476866209134 2314 56-57 0.045181489501032956 2314 58-59 0.479716803719775 2314 60-61 0.7210659186868966 2314 62-63 0.6051659419616087 2314 64-65 0.7002229447102337 2314 66-67 0.7024635774492296 2314 68-69 0.7531721672471718 2314 70-71 0.5281867990671216 2314 72-73 0.7218302775225283 2314 74-75 0.7770486873688398 2314 76-77 0.7317306705771252 2314 78-79 0.508146368757977 2314 80-81 0.6142261149680479 2314 82-83 0.6310134358001527 2314 84-85 0.6536184898255861 2314 86-87 0.7010779380765442 2314 88-89 0.6377286708669914 2314 90-91 0.8256066340466823 2314 92-93 0.7895171151680067 2314 94-95 0.9225923974185442 2314 96-97 0.9519169684286055 2314 98-99 1.0122663998702919 2314 100 -0.41023222261067005 2315 1 0.14062269891427093 2315 2 0.1961741978508158 2315 3 0.3107857250634254 2315 4 0.13130511401458023 2315 5 0.24469059208673372 2315 6 0.31340477501984054 2315 7 0.35657590925583094 2315 8 0.28541877852261166 2315 9 0.46824016631245513 2315 10-11 0.2476734138574912 2315 12-13 0.44975558661660386 2315 14-15 0.18773489223384132 2315 16-17 0.3860125804642962 2315 18-19 0.481873413837441 2315 20-21 0.371415751885273 2315 22-23 0.4062169292402871 2315 24-25 0.4650938872569341 2315 26-27 0.5705131428652237 2315 28-29 0.6368327509389431 2315 30-31 0.605266711445438 2315 32-33 0.6875482843236824 2315 34-35 0.711582720784186 2315 36-37 0.5972135938339491 2315 38-39 0.6480520095010078 2315 40-41 0.7169646197375172 2315 42-43 0.7482607649861492 2315 44-45 0.7989861611771616 2315 46-47 0.6926425000910115 2315 48-49 -4.8315934855802 2315 50-51 -0.8319490189138676 2315 52-53 0.3111198124054724 2315 54-55 0.2358946923403451 2315 56-57 0.5898678313746615 2315 58-59 0.7189585307093793 2315 60-61 0.6576116014241791 2315 62-63 0.4568652949955023 2315 64-65 0.6181034228988977 2315 66-67 -0.14086899524465935 2315 68-69 0.6111422318592687 2315 70-71 0.5784987457547501 2315 72-73 0.7012103141177377 2315 74-75 0.7834220430142693 2315 76-77 0.7518227079498736 2315 78-79 0.7264731310116659 2315 80-81 0.6032743014768371 2315 82-83 0.5966797770821621 2315 84-85 0.616914425052915 2315 86-87 0.6449987808650519 2315 88-89 0.6114921035482901 2315 90-91 0.6939050413522061 2315 92-93 0.7613232957860667 2315 94-95 0.39551566157594564 2315 96-97 0.5436221107738248 2315 98-99 0.48797001248507854 2315 100 0.6518161794126307 2316 1 0.20289648647695913 2316 2 0.24398456720260242 2316 3 0.16695259141194896 2316 4 -0.03687048363715206 2316 5 0.13712023537564733 2316 6 0.14681981519846232 2316 7 0.2438317514210624 2316 8 0.3226449220187604 2316 9 0.4581397215912162 2316 10-11 0.3885773067893865 2316 12-13 0.29031294773545824 2316 14-15 0.41925879388016085 2316 16-17 0.4040646967389492 2316 18-19 0.13141768392579678 2316 20-21 0.40390596601353224 2316 22-23 0.558933612864287 2316 24-25 0.5960654926674067 2316 26-27 0.627292851130882 2316 28-29 0.6429584351871043 2316 30-31 0.5532974612339601 2316 32-33 0.5719626751812115 2316 34-35 0.5238127576910401 2316 36-37 0.41111429956587386 2316 38-39 0.5697617974665476 2316 40-41 0.1975789971070867 2316 42-43 0.1051809687728209 2316 44-45 0.3714585179611234 2316 46-47 0.36872683019024066 2316 48-49 -4.977164407292335 2316 50-51 -0.9530328016226761 2316 52-53 0.2721925182695131 2316 54-55 0.545647931662117 2316 56-57 0.4638122788353627 2316 58-59 0.2626613050982556 2316 60-61 0.41220600054491285 2316 62-63 0.3229249547889097 2316 64-65 0.3479786811847987 2316 66-67 0.4691541818093157 2316 68-69 0.12755943214029486 2316 70-71 -0.32858751737521175 2316 72-73 -0.05674052153926823 2316 74-75 0.23875849902935897 2316 76-77 0.13423311831739326 2316 78-79 0.23205434642289902 2316 80-81 0.2286573185451104 2316 82-83 0.11181433895599113 2316 84-85 0.2434016409911699 2316 86-87 0.28800557004815985 2316 88-89 0.1433322499648746 2316 90-91 0.15098399241744787 2316 92-93 0.30152775442506297 2316 94-95 0.5193271690453294 2316 96-97 0.5328731808632483 2316 98-99 0.5209691292753043 2316 100 -0.27491867692396355 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 9.0 11 10.0 12 48.0 13 208.0 14 784.0 15 2340.0 16 5581.0 17 11083.0 18 18925.0 19 26999.0 20 37002.0 21 51234.0 22 63946.0 23 69751.0 24 74563.0 25 83924.0 26 100382.0 27 124208.0 28 152837.0 29 188012.0 30 231309.0 31 286002.0 32 359671.0 33 466443.0 34 642562.0 35 901374.0 36 1396991.0 37 2041500.0 38 1592396.0 39 191207.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.997068954418275 19.31775848215113 13.506431751666842 24.17874081176375 2 15.795174152687594 20.208517932090462 39.8497050914941 24.14660282372784 3 18.943833841429154 25.428263546764274 29.515669403225044 26.112233208581532 4 12.853975289526176 16.43643473502639 37.50062779560949 33.20896217983795 5 13.500641906225777 37.40065802240258 34.3363259721508 14.762374099220832 6 31.623594665506598 36.40335451594923 17.852961129731717 14.120089688812449 7 28.35989894440671 31.844900450768286 21.647841166779006 18.147359438046003 8 26.511717044626536 35.231366433409725 19.759629057647935 18.49728746431581 9 26.72835914305942 15.894743201476164 20.08712557422104 37.28977208124338 10-11 25.380964599971616 25.98185999539645 27.759730426456585 20.877444978175348 12-13 25.71692928689171 23.500050349102644 28.027089735805077 22.755930628200574 14-15 23.068751170679334 25.062495158999425 25.586038579012776 26.282715091308468 16-17 22.6335207611864 27.861943831942586 26.417246468768802 23.087288938102212 18-19 22.376635831391685 27.109384143244657 28.357454940034703 22.15652508532896 20-21 23.239143311933272 26.36043188080488 28.19950373372781 22.20092107353404 22-23 23.1529269524857 26.153465472450403 28.004435992429144 22.68917158263475 24-25 22.749744321363366 26.280551589417467 28.30311665090779 22.666587438311378 26-27 22.723772931325332 26.479367932964898 28.01802832948027 22.7788308062295 28-29 22.578748485811605 26.598812461881483 28.192754414621813 22.6296846376851 30-31 23.058128102149126 26.38658227037628 28.136640596700218 22.41864903077437 32-33 22.904115744123207 26.574639557779552 27.91457660130771 22.60666809678953 34-35 23.00145412191092 26.573822976529765 28.051706912797535 22.37301598876178 36-37 22.932572443934465 26.411430754546828 27.91896508163759 22.737031719881116 38-39 22.878581625050963 26.55488950252615 28.024762391009755 22.541766481413127 40-41 22.904075946556734 26.228198736142726 28.26075313394667 22.606972183353868 42-43 22.754019866445656 26.286637663907992 27.98667537418224 22.972667095464118 44-45 22.672562675843192 26.599937646095707 27.947059375291065 22.78044030277003 46-47 22.712404272454208 26.371083798368144 27.660698103184277 23.25581382599338 48-49 22.941378014085036 26.520136439235575 27.58403511132504 22.95445043535435 50-51 22.799015494254583 26.602357554845813 27.46923885569519 23.12938809520442 52-53 22.67120486247797 26.723507422998523 27.42126599434407 23.184021720179437 54-55 22.649161227556803 26.620493097839613 27.480544664422226 23.249801010181358 56-57 22.836615250329487 26.587493960792862 27.253578831546292 23.322311957331358 58-59 22.54038839256248 26.593135776880512 27.614070223273423 23.25240560728358 60-61 22.781212858085674 26.800380162165904 27.315261212928256 23.103145766820166 62-63 22.75122323252884 26.70932537012196 27.286649788772316 23.252801608576885 64-65 22.470591673279277 26.493967340669684 27.366985619988487 23.66845536606255 66-67 22.524241095970932 26.49739672578651 27.245836005952157 23.732526172290395 68-69 22.353696639850995 26.581077093125284 27.331235586581514 23.73399068044221 70-71 22.43574201202042 26.335085831391503 27.339194513247804 23.88997764334027 72-73 22.53691730433908 26.598525336505823 27.051896798776827 23.81266056037827 74-75 22.55353819495383 26.695888755197362 26.938249424538004 23.81232362531081 76-77 22.433525719375297 26.597486346263516 26.98102670922939 23.9879612251318 78-79 22.411810091317726 26.536463224404873 26.966123729287624 24.085602954989778 80-81 22.35472424773545 26.847762980608703 26.81900541016184 23.97850736149401 82-83 22.64802667917973 26.545951591054042 27.055689975203588 23.75033175456264 84-85 22.65293847370216 26.639778313644086 26.713699352683733 23.99358385997002 86-87 22.30381774264667 26.77909347021028 26.980058748336074 23.937030038806974 88-89 22.39699144768238 26.41516107174659 26.940263843278302 24.247583637292728 90-91 22.536723194198284 26.40833514172748 26.909168026480373 24.145773637593866 92-93 22.528680846360665 26.513865527168427 26.779529886253574 24.177923740217338 94-95 22.16116665908322 26.366119215042193 26.962737144827532 24.509976981047053 96-97 22.291346628922977 26.334102054331904 26.93142726925227 24.443124047492848 98-99 22.145336687388188 26.383154257118758 26.83044502704057 24.641064028452483 100 22.14298585008555 26.302043606008986 26.779234859889844 24.775735684015622 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2118.0 1 1503.5 2 2007.5 3 2750.5 4 1857.5 5 1438.5 6 2050.5 7 3635.0 8 5429.5 9 6251.5 10 5665.0 11 5009.0 12 5242.5 13 5774.0 14 6478.0 15 7250.0 16 7932.5 17 8595.5 18 9359.0 19 10158.0 20 11154.0 21 12352.0 22 13888.5 23 16233.5 24 19055.5 25 22512.5 26 27864.5 27 34590.5 28 42390.0 29 53587.0 30 69422.0 31 86722.0 32 108748.0 33 134191.5 34 159533.0 35 185334.5 36 214279.0 37 244079.5 38 261137.5 39 268690.5 40 270742.0 41 271097.0 42 276853.5 43 290900.5 44 308652.5 45 331272.0 46 351263.5 47 361986.5 48 374377.0 49 382746.0 50 381909.5 51 376892.0 52 367886.5 53 356813.5 54 344015.0 55 319914.0 56 288697.5 57 255143.5 58 222689.5 59 193699.5 60 160105.0 61 127669.5 62 102561.5 63 80302.0 64 60383.0 65 44229.5 66 32330.5 67 24693.0 68 19097.5 69 14323.5 70 11007.5 71 8497.5 72 6591.5 73 5155.0 74 3894.0 75 2764.0 76 1828.5 77 1121.0 78 730.5 79 481.5 80 306.5 81 184.0 82 112.0 83 64.5 84 38.5 85 26.5 86 18.5 87 13.0 88 8.0 89 7.0 90 4.0 91 2.0 92 1.0 93 1.0 94 0.5 95 1.0 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02992988185151731 2 0.002028215436824433 3 8.77066134842998E-5 4 0.023121655979798535 5 5.372030075913363E-4 6 3.288998005661243E-5 7 3.288998005661243E-5 8 0.0036507877862839797 9 0.010086260550694478 10-11 0.0010689243518399037 12-13 4.9334970084918643E-5 14-15 1.3704158356921844E-4 16-17 3.453447905944305E-4 18-19 1.6444990028306213E-5 20-21 6.577996011322485E-5 22-23 2.5215651376736195E-4 24-25 6.029829677045612E-5 26-27 8.77066134842998E-5 28-29 0.0010524793618115977 30-31 0.002444821850874857 32-33 0.009762842413471123 34-35 0.018922701859237685 36-37 0.021827983430905112 38-39 0.02110440386965964 40-41 0.004730675464809421 42-43 0.005070538592061083 44-45 0.008765179685087212 46-47 0.01133607979284575 48-49 1.2057631138654399 50-51 0.01771673592382856 52-53 0.018517058771872794 54-55 0.009620319166559135 56-57 0.007778480283388838 58-59 0.008255384994209719 60-61 0.005202098512287532 62-63 0.007789443610074376 64-65 0.01919130336303335 66-67 0.02861428264925281 68-69 0.029008962409932157 70-71 0.025610331137415544 72-73 0.022869499466031173 74-75 0.009000891208826267 76-77 0.014120764770972268 78-79 0.008178641707410957 80-81 0.002658606721242838 82-83 0.013687713366893539 84-85 0.023340922513509284 86-87 0.022825646159289024 88-89 0.03194165229831343 90-91 0.01984362130082283 92-93 0.012766793925308391 94-95 0.006292949517498512 96-97 0.0035575995094569105 98-99 0.015485698943321684 100 0.03477567224652487 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9121319.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.98066162925095 #Duplication Level Percentage of deduplicated Percentage of total 1 73.78430398738752 42.04278459055119 2 15.322464028269328 17.461682762423678 3 5.014474338660135 8.571841966192654 4 2.1490152801943925 4.898092500673864 5 1.1419719429740947 3.2535158436352574 6 0.6544218133761213 2.2373632746471346 7 0.425739824979197 1.69812558364653 8 0.26349536146644287 1.201131202607724 9 0.20919226625449008 1.0727922365012943 >10 0.9470978167834071 9.294484743397796 >50 0.051613341178306384 2.0205512210462 >100 0.032866879442127836 3.6074407378028077 >500 0.0023208695355320694 0.8973838902928555 >1k 9.393644047157313E-4 0.879284008309412 >5k 0.0 0.0 >10k+ 8.288509421374017E-5 0.8635254382716133 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23628 0.2590414829258795 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18829 0.20642847816198512 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12395 0.13589043426723701 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0963326685537476E-5 0.0 5 0.0 0.0 0.0 1.0963326685537476E-5 1.0963326685537476E-5 6 1.315599202264497E-4 0.0 0.0 1.0963326685537476E-5 3.288998005661243E-5 7 1.315599202264497E-4 0.0 0.0 1.0963326685537476E-5 3.288998005661243E-5 8 1.315599202264497E-4 0.0 0.0 1.0963326685537476E-5 3.288998005661243E-5 9 1.4252324691198717E-4 0.0 0.0 2.1926653371074952E-5 3.288998005661243E-5 10-11 1.589682369402934E-4 0.0 0.0 2.1926653371074952E-5 3.288998005661243E-5 12-13 1.6444990028306213E-4 0.0 0.0 3.288998005661243E-5 5.4816633427687376E-5 14-15 1.7541322696859962E-4 0.0 0.0 7.674328679876233E-5 9.318827682706854E-5 16-17 2.740831671384369E-4 0.0 0.0 1.0415160351260602E-4 9.866994016983727E-5 18-19 2.8504649382397434E-4 0.0 0.0 1.6993156362583086E-4 1.2059659354091223E-4 20-21 2.8504649382397434E-4 0.0 0.0 3.34381463908893E-4 1.315599202264497E-4 22-23 2.9600982050951186E-4 0.0 0.0 8.112861747297732E-4 1.425232469119872E-4 24-25 3.069731471950493E-4 0.0 0.0 0.0018966555165979833 1.5348657359752466E-4 26-27 3.069731471950493E-4 0.0 0.0 0.003491819549343686 1.5348657359752466E-4 28-29 3.069731471950493E-4 0.0 0.0 0.008633619764860762 1.5348657359752466E-4 30-31 3.179364738805868E-4 0.0 0.0 0.024601705082346097 1.6993156362583086E-4 32-33 3.2889980056612425E-4 0.0 0.0 0.061230179538726795 2.192665337107495E-4 34-35 3.2889980056612425E-4 0.0 0.0 0.11620029954001171 2.192665337107495E-4 36-37 3.2889980056612425E-4 0.0 0.0 0.20000945038760293 2.30229860396287E-4 38-39 3.2889980056612425E-4 1.0963326685537476E-5 0.0 0.3220806113677199 2.30229860396287E-4 40-41 3.398631272516617E-4 1.0963326685537476E-5 0.0 0.4547861992328083 2.30229860396287E-4 42-43 3.398631272516617E-4 1.0963326685537476E-5 0.0 0.5846029505162575 2.4119318708182448E-4 44-45 3.398631272516617E-4 1.0963326685537476E-5 0.0 0.7396298715131002 2.631198404528994E-4 46-47 3.398631272516617E-4 1.0963326685537476E-5 0.0 0.9215772411862801 2.740831671384369E-4 48-49 3.398631272516617E-4 1.0963326685537476E-5 0.0 1.1174096641066935 2.8504649382397434E-4 50-51 3.617897806227367E-4 1.6444990028306213E-5 0.0 1.327921981459041 2.8504649382397434E-4 52-53 3.617897806227367E-4 2.1926653371074952E-5 0.0 1.5349369975987024 2.9052815716674313E-4 54-55 3.617897806227367E-4 2.1926653371074952E-5 0.0 1.761927194959413 3.014914838522806E-4 56-57 3.617897806227367E-4 2.1926653371074952E-5 0.0 2.022530951937982 3.1245481053781806E-4 58-59 3.617897806227367E-4 2.1926653371074952E-5 0.0 2.3112775685183253 3.179364738805868E-4 60-61 3.782347706510429E-4 2.1926653371074952E-5 0.0 2.6033734814011 3.234181372233555E-4 62-63 4.2756974073596154E-4 2.1926653371074952E-5 0.0 2.901937757028342 3.617897806227367E-4 64-65 4.2756974073596154E-4 2.1926653371074952E-5 0.0 3.2024096514988676 3.617897806227367E-4 66-67 4.60459720792574E-4 2.1926653371074952E-5 0.0 3.5409352528948936 3.6727144396550543E-4 68-69 4.60459720792574E-4 2.1926653371074952E-5 0.0 3.9131127855521775 3.7275310730827416E-4 70-71 4.7142304747811145E-4 2.1926653371074952E-5 0.0 4.305029787906771 4.220880773931928E-4 72-73 5.372030075913363E-4 2.1926653371074952E-5 0.0 4.696069724126522 4.8238637416364897E-4 74-75 5.372030075913363E-4 2.1926653371074952E-5 0.0 5.078366407314556 5.043130275347239E-4 76-77 5.372030075913363E-4 2.1926653371074952E-5 0.0 5.4885702385806265 5.043130275347239E-4 78-79 5.372030075913363E-4 2.1926653371074952E-5 0.0 5.934519996504891 5.043130275347239E-4 80-81 5.372030075913363E-4 2.1926653371074952E-5 0.0 6.402456706097002 5.317213442485676E-4 82-83 5.42684670934105E-4 2.1926653371074952E-5 0.0 6.875233724420777 5.920196410190237E-4 84-85 5.481663342768738E-4 2.1926653371074952E-5 0.0 7.342742864272152 5.920196410190237E-4 86-87 5.536479976196425E-4 2.1926653371074952E-5 0.0 7.826242016094383 5.975013043617925E-4 88 5.700929876479487E-4 2.1926653371074952E-5 0.0 8.203309192453416 6.139462943900986E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13375 0.0 46.284733 1 GTATCAA 23735 0.0 40.675396 1 TACCTGG 7290 0.0 34.047024 2 CAACGCA 29555 0.0 31.728148 5 ATCAACG 29785 0.0 31.47585 3 TCAACGC 30980 0.0 30.413347 4 TATCAAC 31355 0.0 30.391638 2 AACGCAG 31855 0.0 29.805456 6 GTACCTG 9780 0.0 26.298347 1 GTACATG 35040 0.0 26.199411 1 ACCTGGG 9425 0.0 25.987751 3 ACGCAGA 36605 0.0 25.719934 7 TACATGG 36355 0.0 25.050268 2 CGCAGAG 37725 0.0 24.844435 8 ACATGGG 35990 0.0 24.523727 3 GTACTAG 2890 0.0 23.548157 1 CATGGGG 23855 0.0 22.266344 4 GCAGAGT 41785 0.0 22.104033 9 CTAACGC 1525 0.0 21.845806 3 TCTAACG 1595 0.0 21.179848 2 >>END_MODULE