FastQCFastQC Report
Mon 6 Jun 2016
ERR1211362_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211362_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5418148
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232970.42998087169268906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182990.3377353294889693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109790.20263381509696674No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66680.12306788223577503No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97850.054.3611871
GTATCAA183000.042.610891
TACCTGG52800.042.0408172
ACCTGGG60550.035.4650043
ATCAACG223400.034.3269273
CAACGCA226600.033.717445
TATCAAC229650.033.625582
TCAACGC234200.032.8035474
AACGCAG239950.032.175026
GTACCTG71050.032.1706121
GTACATG259900.031.7040711
TACATGG270500.030.0601272
ACATGGG273350.028.9309713
ACGCAGA267500.028.6158927
CATGGGG197450.027.914564
CGCAGAG275100.027.7225998
ATGGGGG145850.027.287955
TGGGGGG147150.025.7061826
GTGGTAT47050.025.3899151
GCAGAGT296400.025.1778349
Produced by FastQC (version 0.11.5)