FastQCFastQC Report
Mon 6 Jun 2016
ERR1211362_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211362_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5418148
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201590.37206440281808467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169540.31291134904399065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105630.19495591482550864No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55380.10221204736378556No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104450.051.3951
TACCTGG52450.044.6327972
GTATCAA180400.039.8237341
ACCTGGG62800.036.8320963
GTACCTG71700.033.2466471
ATCAACG228000.030.9082363
CAACGCA230700.030.6634275
TATCAAC235300.030.1260072
TCAACGC237450.029.7967074
AACGCAG246000.029.1376976
GTACATG264550.028.628531
TACATGG273000.027.4779852
TGGGGGG131450.026.9791456
ACATGGG272600.026.6086463
GTGGTAT49850.026.4511431
ATGGGGG142100.026.0795425
CATGGGG191850.026.069614
CCTGGGG61600.025.8962784
ACGCAGA280450.025.2908427
CGCAGAG285700.024.8096838
Produced by FastQC (version 0.11.5)