##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211362_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5418148 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08064526845705 33.0 31.0 34.0 30.0 34.0 2 32.20113145672654 33.0 31.0 34.0 30.0 34.0 3 32.26294372172927 34.0 31.0 34.0 30.0 34.0 4 35.918043213289856 37.0 35.0 37.0 35.0 37.0 5 35.822463690545185 37.0 35.0 37.0 35.0 37.0 6 35.8423038647154 37.0 35.0 37.0 35.0 37.0 7 35.760814765488135 37.0 35.0 37.0 33.0 37.0 8 35.739142969147395 37.0 35.0 37.0 33.0 37.0 9 37.34543187081638 39.0 37.0 39.0 34.0 39.0 10-11 37.28850319334208 39.0 37.0 39.0 34.0 39.0 12-13 37.20610418910668 39.0 37.0 39.0 33.0 39.0 14-15 38.412968601079186 40.0 38.0 41.0 33.5 41.0 16-17 38.38586690507532 40.0 38.0 41.0 33.0 41.0 18-19 38.39791382590509 40.0 38.0 41.0 33.5 41.0 20-21 38.37355338023251 40.0 38.0 41.0 34.0 41.0 22-23 38.25896690160549 40.0 38.0 41.0 34.0 41.0 24-25 38.16277471564084 40.0 37.0 41.0 33.0 41.0 26-27 38.05531022777525 40.0 37.0 41.0 33.0 41.0 28-29 37.93585474224772 40.0 37.0 41.0 33.0 41.0 30-31 37.92690906560692 40.0 37.0 41.0 33.0 41.0 32-33 37.835443310149515 40.0 37.0 41.0 33.0 41.0 34-35 37.69275756217807 40.0 37.0 41.0 33.0 41.0 36-37 37.54775183328326 40.0 36.0 41.0 32.5 41.0 38-39 37.427455746871445 40.0 36.0 41.0 32.0 41.0 40-41 37.3099983610636 40.0 36.0 41.0 31.5 41.0 42-43 37.199957716179036 39.0 36.0 41.0 31.0 41.0 44-45 37.04642933341799 39.0 35.5 41.0 31.0 41.0 46-47 36.795758439968786 39.0 35.0 41.0 30.5 41.0 48-49 35.23527402721373 39.0 35.0 40.0 24.0 41.0 50-51 35.959087957730205 38.5 34.5 40.0 28.0 41.0 52-53 36.05544828232821 38.5 35.0 40.0 29.0 41.0 54-55 35.88845422827136 38.0 35.0 40.0 28.0 41.0 56-57 35.67895838208923 38.0 34.5 40.0 28.0 41.0 58-59 35.44363627571635 38.0 34.0 40.0 28.0 41.0 60-61 35.177974466552044 37.5 34.0 40.0 27.0 41.0 62-63 34.84579306434597 37.0 34.0 40.0 26.5 41.0 64-65 34.508818419135096 36.0 33.0 39.0 26.0 41.0 66-67 34.16840302258262 36.0 33.0 39.0 26.0 40.0 68-69 33.804385373009374 35.0 33.0 38.5 26.0 40.0 70-71 33.43451341676159 35.0 32.0 38.0 25.5 40.0 72-73 33.050684938838884 35.0 32.0 37.0 25.0 39.5 74-75 32.68011846483337 35.0 32.0 37.0 24.5 39.0 76-77 31.749246329188498 34.0 30.5 35.5 23.5 38.0 78-79 32.1991732230275 34.5 31.0 36.0 24.5 37.5 80-81 32.0860401007872 34.5 31.0 35.0 24.5 37.0 82-83 31.822973735674992 34.0 31.0 35.0 24.0 37.0 84-85 31.54206566524207 34.0 31.0 35.0 23.5 36.0 86-87 31.29374640559837 34.0 31.0 35.0 23.0 36.0 88-89 31.0863718562136 34.0 31.0 35.0 22.5 35.5 90-91 30.87327348754593 34.0 30.0 35.0 20.0 35.0 92-93 30.678795226708463 34.0 30.0 35.0 20.0 35.0 94-95 30.488901189114806 34.0 30.0 35.0 20.0 35.0 96-97 30.30437356085511 34.0 29.0 35.0 19.0 35.0 98-99 30.09167698999732 34.0 29.0 35.0 19.0 35.0 100 27.667511297218162 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.25430419907584323 1101 2 -0.1880491663324868 1101 3 -0.25210421180716835 1101 4 -0.0906837262122977 1101 5 -0.07973476995640993 1101 6 -0.18305686700622203 1101 7 -0.176000576823931 1101 8 -0.08562107429369092 1101 9 -0.2324411030985587 1101 10-11 -0.2330844378734298 1101 12-13 -0.2220233446372788 1101 14-15 -0.3741577531021676 1101 16-17 -0.4249857056970967 1101 18-19 -0.42762110797853836 1101 20-21 -0.3762875207002452 1101 22-23 -0.46618174844283544 1101 24-25 -0.5111575735035316 1101 26-27 -0.5434914557016626 1101 28-29 -0.5468275713942674 1101 30-31 -0.45001447336169065 1101 32-33 -0.4092801292322932 1101 34-35 -0.3867806182745639 1101 36-37 -0.37129824863134786 1101 38-39 -0.3111101226012991 1101 40-41 -0.3290322106593564 1101 42-43 -0.3674429990417778 1101 44-45 -0.3402994910097945 1101 46-47 -0.45685840290602897 1101 48-49 0.7538871426797442 1101 50-51 -0.0806962115747396 1101 52-53 -0.4227002608743291 1101 54-55 -0.37892212043394835 1101 56-57 -0.4218390374770422 1101 58-59 -0.51644891652181 1101 60-61 -0.5468492783035259 1101 62-63 -0.6433758929484341 1101 64-65 -0.6813889144400278 1101 66-67 -0.5997588859611085 1101 68-69 -0.5174252467479334 1101 70-71 -0.5023620700949394 1101 72-73 -0.6368255742328586 1101 74-75 -0.7168205316761345 1101 76-77 -0.6171302668079157 1101 78-79 -0.4402231311167881 1101 80-81 -0.4321479624033273 1101 82-83 -0.5149886319450765 1101 84-85 -0.45206941730146255 1101 86-87 -0.5566161681000246 1101 88-89 -0.5046179034615257 1101 90-91 -0.638810175183405 1101 92-93 -0.6738881196991606 1101 94-95 -0.6092184734434056 1101 96-97 -0.5926342520131023 1101 98-99 -0.9224147953730331 1101 100 -0.6672220126742978 1102 1 -0.26355224750301787 1102 2 -0.2034148386224608 1102 3 -0.27690102716190523 1102 4 -0.17722832347836714 1102 5 -0.14777286071795004 1102 6 -0.27208595564216864 1102 7 -0.21459746131026947 1102 8 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-0.631598288551281 1102 72-73 -0.6561549779849614 1102 74-75 -0.8028579156512237 1102 76-77 -0.652169663630815 1102 78-79 -0.5602669084274652 1102 80-81 -0.5745720350114993 1102 82-83 -0.6199125158011967 1102 84-85 -0.5882163268429572 1102 86-87 -0.6403650336847377 1102 88-89 -0.6473185929794738 1102 90-91 -0.5796416412600252 1102 92-93 -0.6647430568625232 1102 94-95 -0.7171121728936001 1102 96-97 -0.850642574878151 1102 98-99 -0.8631383179031964 1102 100 -0.8553603520701181 1103 1 -0.3431356298527106 1103 2 -0.28484757570693375 1103 3 -0.3086470868972917 1103 4 -0.15118407348157348 1103 5 -0.23088500116562471 1103 6 -0.25955006873909525 1103 7 -0.280428553325919 1103 8 -0.2961809302649172 1103 9 -0.48033658640180477 1103 10-11 -0.34504802039461424 1103 12-13 -0.49978741715379726 1103 14-15 -0.5273735689154364 1103 16-17 -0.5586454446973477 1103 18-19 -0.560875179564178 1103 20-21 -0.6162044206475059 1103 22-23 -0.6266935257752237 1103 24-25 -0.8060845221733146 1103 26-27 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1105 68-69 -0.595140139886638 1105 70-71 -0.6328116472791194 1105 72-73 -0.4942638061500162 1105 74-75 -0.6132795698674585 1105 76-77 -0.6737618910634424 1105 78-79 -0.5296887064554525 1105 80-81 -0.5186308960377204 1105 82-83 -0.44216877172364377 1105 84-85 -0.43880378152540445 1105 86-87 -0.3862520598016843 1105 88-89 -0.4980117773556323 1105 90-91 -0.6699856968637938 1105 92-93 -0.5932737192607682 1105 94-95 -0.5396709057485509 1105 96-97 -0.5123062633942368 1105 98-99 -0.5166154928622895 1105 100 -0.6243090647127154 1106 1 -0.29237676569190896 1106 2 -0.28768287277000937 1106 3 -0.2973224220159594 1106 4 -0.22862972070323195 1106 5 -0.22412827888728515 1106 6 -0.2714919706228329 1106 7 -0.26398735372594473 1106 8 -0.33190401282288207 1106 9 -0.4363811392417247 1106 10-11 -0.4335456158796589 1106 12-13 -0.4633976692251096 1106 14-15 -0.6535050249670391 1106 16-17 -0.5668047095822928 1106 18-19 -0.5996128053614953 1106 20-21 -0.6044139020007648 1106 22-23 -0.455299109589248 1106 24-25 -0.4752974889048929 1106 26-27 -0.557845888053798 1106 28-29 -0.6491321080311891 1106 30-31 -0.6874616329424654 1106 32-33 -0.6471776595101204 1106 34-35 -0.6483098203740667 1106 36-37 -0.6846625679449119 1106 38-39 -0.6809283046071002 1106 40-41 -0.6179677446831136 1106 42-43 -0.5907460580182899 1106 44-45 -0.6701851617442216 1106 46-47 -0.7531457449275223 1106 48-49 0.4953841855927834 1106 50-51 -0.4053938381062778 1106 52-53 -0.7270925291277024 1106 54-55 -0.9744797488126196 1106 56-57 -0.8749523025620007 1106 58-59 -0.7871193836464201 1106 60-61 -0.8647493931277168 1106 62-63 -0.7994132753633778 1106 64-65 -0.8185898475596929 1106 66-67 -0.7914216521932929 1106 68-69 -0.7597182124597097 1106 70-71 -0.856411502957279 1106 72-73 -0.9771140440563997 1106 74-75 -0.9513106241359814 1106 76-77 -0.866860034467436 1106 78-79 -0.6854588759168081 1106 80-81 -0.6622389878875801 1106 82-83 -0.6611179780917595 1106 84-85 -0.6353038913397313 1106 86-87 -0.6777858094755622 1106 88-89 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0.35270708155491803 2311 2 0.3751927013606391 2311 3 0.42142911876404554 2311 4 0.2884426019894093 2311 5 0.34989373801855095 2311 6 0.38310101052794465 2311 7 0.38446805634084313 2311 8 0.20319263450385705 2311 9 0.4664992949492799 2311 10-11 0.37052716592540946 2311 12-13 0.45894546576354855 2311 14-15 0.39823763323624917 2311 16-17 0.4840238538696653 2311 18-19 0.6428518919668775 2311 20-21 0.690100665053663 2311 22-23 0.7375195749416008 2311 24-25 0.6816754970995476 2311 26-27 0.7508022339714984 2311 28-29 0.7266236025397319 2311 30-31 0.6207202210392992 2311 32-33 0.6095816503228875 2311 34-35 0.7533195644857287 2311 36-37 0.847365085734495 2311 38-39 0.865646569457148 2311 40-41 0.8262384902812769 2311 42-43 0.8301554031374039 2311 44-45 0.8642930765191181 2311 46-47 0.9262725557930338 2311 48-49 -6.183311909518409 2311 50-51 -1.1035681458678397 2311 52-53 0.3491336916343002 2311 54-55 0.8101947968293715 2311 56-57 0.9139936694468673 2311 58-59 0.9178878392100884 2311 60-61 0.773163351820287 2311 62-63 0.6867219617390461 2311 64-65 0.8015190523698124 2311 66-67 0.9480237726585798 2311 68-69 0.943312873997499 2311 70-71 0.9919391660854231 2311 72-73 0.5980675678828291 2311 74-75 0.8286037036933394 2311 76-77 0.9139924002691231 2311 78-79 0.8779809678697106 2311 80-81 0.8822906267845525 2311 82-83 0.8978409892958901 2311 84-85 0.9906459014833864 2311 86-87 0.939233910587518 2311 88-89 0.9552008915832282 2311 90-91 1.016367649462726 2311 92-93 0.977845291631187 2311 94-95 0.9534925903609128 2311 96-97 0.9573466268804189 2311 98-99 1.1005890137223666 2311 100 1.2339898018348592 2312 1 0.011287337210660553 2312 2 0.19873227974372298 2312 3 0.2183509166417963 2312 4 -0.052513720428464694 2312 5 0.15045734510389508 2312 6 0.10629492388613926 2312 7 0.26173100828868456 2312 8 0.34328689247801947 2312 9 0.2748115159650837 2312 10-11 0.24698653848306407 2312 12-13 0.2519496829817527 2312 14-15 0.34798546561756183 2312 16-17 0.4691984173298849 2312 18-19 0.35268970740587946 2312 20-21 0.324848130264229 2312 22-23 0.5828839132212522 2312 24-25 0.7276805483198601 2312 26-27 0.6216593678367062 2312 28-29 0.7268896701935645 2312 30-31 0.7529443053416998 2312 32-33 0.7283394854842058 2312 34-35 0.3046858330344051 2312 36-37 0.6732958635594741 2312 38-39 0.7677897975173806 2312 40-41 0.5602850527763579 2312 42-43 0.6453518132566316 2312 44-45 0.7548186289799474 2312 46-47 0.8413256381913996 2312 48-49 -4.6870557397386605 2312 50-51 -0.8055125771990461 2312 52-53 0.5201087135334177 2312 54-55 0.7763951566566902 2312 56-57 0.8986366221290538 2312 58-59 0.9263502068319198 2312 60-61 0.9490595106898212 2312 62-63 0.7612134302646467 2312 64-65 0.9598415017133632 2312 66-67 0.9437752138037325 2312 68-69 0.8182391775847506 2312 70-71 0.8760415909858921 2312 72-73 0.9434750487243875 2312 74-75 0.6641834662912274 2312 76-77 0.8704177570804035 2312 78-79 0.4924499865272338 2312 80-81 0.6704470100478588 2312 82-83 0.7036590719302858 2312 84-85 0.8065198727496572 2312 86-87 0.9015991720393117 2312 88-89 0.9591273892464507 2312 90-91 0.6783557741119495 2312 92-93 0.8873677336313257 2312 94-95 0.9528011291787273 2312 96-97 1.0339073647886998 2312 98-99 1.0999030601012763 2312 100 0.6133419552912471 2313 1 0.07809804360228867 2313 2 0.2090988539555596 2313 3 0.28560644709372696 2313 4 0.09741357088996239 2313 5 0.16218778111107213 2313 6 0.26391280379282733 2313 7 0.18894502591020768 2313 8 0.23958471948340332 2313 9 0.43138805448079154 2313 10-11 0.1995281278489216 2313 12-13 0.011785101140866061 2313 14-15 0.10694814999146729 2313 16-17 0.06035160763376268 2313 18-19 0.33794959119444457 2313 20-21 0.3905498379232881 2313 22-23 0.4225402996457035 2313 24-25 0.2893794517198742 2313 26-27 0.44037067916456607 2313 28-29 0.35779369167247665 2313 30-31 0.47928030823167234 2313 32-33 0.41204095435264776 2313 34-35 0.3977889171070714 2313 36-37 -0.3599222073797108 2313 38-39 0.10867396569944532 2313 40-41 0.3926331452066947 2313 42-43 0.4550118886899739 2313 44-45 0.3817952336222774 2313 46-47 0.3242752227569454 2313 48-49 -5.566405019999248 2313 50-51 -1.5015716901225176 2313 52-53 -0.02995412207455672 2313 54-55 0.2028888225824943 2313 56-57 0.19432553306015166 2313 58-59 0.26057257600221106 2313 60-61 0.6105687592341127 2313 62-63 0.6474539628289548 2313 64-65 0.7033644592882311 2313 66-67 0.7866215214272785 2313 68-69 0.8247366549761779 2313 70-71 0.7102568423589375 2313 72-73 0.5869314969894859 2313 74-75 0.703715635673376 2313 76-77 0.7362879192743392 2313 78-79 0.6027832678164486 2313 80-81 -0.19690411318646284 2313 82-83 0.14476840741885866 2313 84-85 0.5206914070712152 2313 86-87 0.6194882629818039 2313 88-89 0.6619770338157984 2313 90-91 0.6305351211726808 2313 92-93 0.7010055096947063 2313 94-95 0.6949565512564533 2313 96-97 0.6832090553038164 2313 98-99 0.8222627399993918 2313 100 1.0332511230141037 2314 1 0.04473803311737612 2314 2 0.1741582334861036 2314 3 0.18636047026010516 2314 4 0.017434525725050776 2314 5 0.18538040784029164 2314 6 0.2671990119903782 2314 7 0.10693167923680136 2314 8 0.2456307907292441 2314 9 0.37885176065843496 2314 10-11 0.3337395428506298 2314 12-13 0.23423715574640624 2314 14-15 0.2110187699150856 2314 16-17 0.41071096940952856 2314 18-19 0.4512105198292389 2314 20-21 0.5427522099854301 2314 22-23 0.23317751017836486 2314 24-25 0.4376601775303399 2314 26-27 0.4805411235198349 2314 28-29 0.5778006781170646 2314 30-31 0.4279705785541452 2314 32-33 0.3916771703778963 2314 34-35 0.2980864745656717 2314 36-37 0.4053958610445676 2314 38-39 0.5078694973740312 2314 40-41 0.2907031455126017 2314 42-43 0.3034659110166089 2314 44-45 0.5291288380758488 2314 46-47 0.5922686540894446 2314 48-49 -6.186180969414927 2314 50-51 -1.5803939381171546 2314 52-53 -0.8433015227692735 2314 54-55 -0.40239902673223327 2314 56-57 -0.03326161080212131 2314 58-59 0.46836044481120354 2314 60-61 0.6589051636639311 2314 62-63 0.5142101006731963 2314 64-65 0.704069701805885 2314 66-67 0.7611706715673066 2314 68-69 0.6461764998738104 2314 70-71 0.5288206833769138 2314 72-73 0.6902567611967569 2314 74-75 0.697389034459448 2314 76-77 0.6435867294643813 2314 78-79 0.5147244122317645 2314 80-81 0.5199966121201456 2314 82-83 0.5106087271967787 2314 84-85 0.5692959701087652 2314 86-87 0.651311900467654 2314 88-89 0.5142025965552399 2314 90-91 0.730051206040514 2314 92-93 0.7819665784482801 2314 94-95 0.8805585971345522 2314 96-97 0.9032055823255654 2314 98-99 0.9132595687323786 2314 100 -0.3598767437674901 2315 1 0.12578816760354528 2315 2 0.2018236633448538 2315 3 0.3268976533744379 2315 4 0.13111769618645752 2315 5 0.24127304836338226 2315 6 0.32042346567585156 2315 7 0.34166174998853904 2315 8 0.2659702477700705 2315 9 0.4280275846983983 2315 10-11 0.22157967120962496 2315 12-13 0.4069604351814107 2315 14-15 0.25558015312229543 2315 16-17 0.357040354946939 2315 18-19 0.4676218877803393 2315 20-21 0.3643228373551324 2315 22-23 0.4012253738040954 2315 24-25 0.4572373608227096 2315 26-27 0.5530872145194294 2315 28-29 0.596107586487939 2315 30-31 0.6195934302741861 2315 32-33 0.6616790025943331 2315 34-35 0.6216050249536025 2315 36-37 0.5800167455427214 2315 38-39 0.6243104008520746 2315 40-41 0.6476625761358932 2315 42-43 0.6834328989763989 2315 44-45 0.6123882027789023 2315 46-47 0.5098935007247718 2315 48-49 -4.85292633979217 2315 50-51 -0.9734136014344017 2315 52-53 0.16240322284441078 2315 54-55 0.025723270377604024 2315 56-57 0.46174749093707135 2315 58-59 0.5881348448159613 2315 60-61 0.48809983901050913 2315 62-63 0.20822237528708598 2315 64-65 0.43382710991935625 2315 66-67 -0.2805858379935984 2315 68-69 0.48112112045170363 2315 70-71 0.5532272405853362 2315 72-73 0.5616822330110338 2315 74-75 0.7764162387938711 2315 76-77 0.6718995919205568 2315 78-79 0.6183966091755053 2315 80-81 0.4915232143699413 2315 82-83 0.48178919878605697 2315 84-85 0.5081527684230274 2315 86-87 0.49674440245441076 2315 88-89 0.5034029787244414 2315 90-91 0.644383997374053 2315 92-93 0.7575301271820258 2315 94-95 0.2636122640153502 2315 96-97 0.38954797882511727 2315 98-99 0.3463853744996328 2315 100 0.5100678347757466 2316 1 0.21586359329323557 2316 2 0.24167686789824927 2316 3 0.15873721435636412 2316 4 -0.02432470590307645 2316 5 0.13286334051270643 2316 6 0.1661305295958826 2316 7 0.2399313815583568 2316 8 0.2983623685098138 2316 9 0.4311695887689879 2316 10-11 0.347981777851345 2316 12-13 0.28908788187400347 2316 14-15 0.4261506958109891 2316 16-17 0.44112631628852483 2316 18-19 0.11079093954723618 2316 20-21 0.4151479509090308 2316 22-23 0.5736145503275765 2316 24-25 0.5827168649238388 2316 26-27 0.6134428538541528 2316 28-29 0.6671744922754641 2316 30-31 0.5799356312774151 2316 32-33 0.6282681952438125 2316 34-35 0.5681915055034423 2316 36-37 0.4561464816383278 2316 38-39 0.6064774295392468 2316 40-41 0.20412629141917904 2316 42-43 0.06520532938416324 2316 44-45 0.33918454120006913 2316 46-47 0.46917847153915915 2316 48-49 -4.9659330565145865 2316 50-51 -0.9441997071769705 2316 52-53 0.3524520162044098 2316 54-55 0.6212819018179303 2316 56-57 0.612861284284854 2316 58-59 0.517451603392665 2316 60-61 0.6155955204194328 2316 62-63 0.6159064743202549 2316 64-65 0.5308981034029898 2316 66-67 0.6268669384878578 2316 68-69 0.34590255209823795 2316 70-71 -0.03582296257780371 2316 72-73 0.0070857738892158295 2316 74-75 0.24037597755270212 2316 76-77 0.2691987316230069 2316 78-79 0.3627086521195224 2316 80-81 0.3281798674435308 2316 82-83 0.2597902836259074 2316 84-85 0.4015940685693842 2316 86-87 0.3835926128351552 2316 88-89 0.25636551998622537 2316 90-91 0.27645610257420117 2316 92-93 0.3959990614246003 2316 94-95 0.5692628991597672 2316 96-97 0.6089702771707373 2316 98-99 0.6447123022282817 2316 100 -0.19059846213319176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 3.0 11 11.0 12 28.0 13 142.0 14 572.0 15 1609.0 16 3724.0 17 8114.0 18 13506.0 19 19288.0 20 26149.0 21 35685.0 22 44085.0 23 47504.0 24 48897.0 25 53230.0 26 61732.0 27 74511.0 28 91898.0 29 111978.0 30 137142.0 31 170485.0 32 214409.0 33 279043.0 34 395789.0 35 538514.0 36 812547.0 37 1192707.0 38 928703.0 39 106136.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.532720128996665 19.038418011089668 13.71291151934784 23.715950340565826 2 16.14018075097701 20.39011366056719 40.067075644390926 23.40262994406487 3 18.947133926303916 26.55093699982872 29.952175505117616 24.54975356874974 4 13.184029914834433 16.58107158640851 37.072275353527374 33.16262314522969 5 13.296704371647936 37.679548079157385 33.39545823761281 15.628289311581863 6 30.54290721270841 35.78268946290659 19.04945327229153 14.624950052093475 7 27.969187995602923 32.61287805353416 21.831131227866052 17.58680272299686 8 26.958569999988924 34.99203943625994 19.966581505430643 18.082809058320496 9 28.06206830355989 16.087035170685066 20.17734855784317 35.67354796791187 10-11 25.827131427976596 25.89089002348243 27.74608150875225 20.535897039788722 12-13 25.785301347712675 23.667583155335695 27.93090732378234 22.616208173169294 14-15 23.51035378281534 24.957298541815224 26.10482323225099 25.42752444311845 16-17 22.649618475448452 27.543750787863935 26.781578370844116 23.0250523658435 18-19 22.38639792770404 26.753501428994607 28.714477002738022 22.14562364056333 20-21 23.299459501360705 26.250589454508876 28.38938418960733 22.06056685452309 22-23 23.102345560400504 25.808639309931593 28.4894999279733 22.599515201694604 24-25 22.514533105137236 25.744438089258193 28.82221541792794 22.918813387676636 26-27 22.47625962656766 26.047169108754687 28.57829439179675 22.898276872880903 28-29 22.375705979809677 26.74754140780779 28.620839758951977 22.25591285343056 30-31 23.480237452890833 26.02974787195499 28.716329140581554 21.773685534572625 32-33 23.453259918562026 26.012427966524044 28.4064589247677 22.127853190146226 34-35 23.130970988148526 26.416363200373038 28.45112108235891 22.00154472911953 36-37 22.955448255135423 26.34697696983302 28.26513914943782 22.432435625593737 38-39 22.912244247324303 26.589564964082523 28.20719434978875 22.290996438804424 40-41 22.98301500894904 25.953215091970115 28.74944558787619 22.314324311204654 42-43 22.560530832979566 26.36106240425257 28.18668672363831 22.891720039129552 44-45 22.04735360748541 26.715610067138435 28.530019224995762 22.70701710038039 46-47 22.216160625923447 26.131273994438242 28.12789367260716 23.524671707031146 48-49 22.627434233066896 26.41653580346271 27.931261043042316 23.024768920428073 50-51 22.66610463234362 26.468253905989332 27.9102374453491 22.95540401631795 52-53 22.44784486995657 26.72100045549929 27.66784825735756 23.163306417186583 54-55 22.382086777427745 26.42154552738875 28.062795990923668 23.133571704259833 56-57 22.6454728490551 26.393134223450787 27.541986358817155 23.419406568676962 58-59 22.24408573153604 26.530108793339167 28.08130124519561 23.14450422992918 60-61 22.834838310162272 26.739287624305362 27.790127370459633 22.63574669507273 62-63 22.88014029449329 26.699735766522114 27.58921998288403 22.830903956100567 64-65 22.34324568270126 26.333060218309267 27.582596065833 23.741098033156476 66-67 22.393731724614817 26.258040200239652 27.43431107595997 23.91391699918556 68-69 21.85612978168609 26.38626526879203 27.90512284080579 23.85248210871609 70-71 22.20091703876637 26.00621069738256 28.07099712135413 23.72187514249694 72-73 22.084793194332477 26.661287818468764 27.403843935005597 23.850075052193166 74-75 22.282784399070856 26.541889037241045 27.231761952036713 23.943564611651386 76-77 21.876357898599654 26.324016895258183 27.54701628721496 24.2526089189272 78-79 21.659484031588335 26.217916250745706 27.73441883782331 24.38818087984265 80-81 21.80810046194065 26.60521054586073 27.306750151162458 24.27993884103616 82-83 22.175775430026363 26.462273297695894 27.48609834239652 23.875852929881226 84-85 22.036283342103737 26.651483917858087 27.11308612583947 24.19914661419871 86-87 21.553136294136408 26.74673951218792 27.459983191625163 24.24014100205051 88-89 21.46585655643086 26.32686875359379 27.53152909983994 24.67574559013541 90-91 21.988339416284095 25.972046055299806 27.604520391437926 24.43509413697817 92-93 21.74064687380017 26.20191455742934 27.466977022357426 24.590461546413067 94-95 21.366691403031805 26.029027098548035 27.475066214613513 25.129215283806644 96-97 21.16712734269008 25.972022410348135 27.637405225802002 25.22344502115978 98-99 20.91511369583215 26.008633444120278 27.53645954953616 25.539793310511406 100 20.751927702568473 26.167311511270068 27.622070597375476 25.458690188785983 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2239.0 1 1648.5 2 2692.5 3 3780.0 4 2393.5 5 1646.5 6 2286.5 7 4061.5 8 6194.0 9 7149.5 10 6319.0 11 5109.5 12 5058.5 13 5600.5 14 6023.0 15 6539.0 16 7214.0 17 7720.0 18 8264.0 19 8829.5 20 9324.5 21 9962.0 22 10969.5 23 12431.0 24 14243.5 25 16506.5 26 19740.0 27 23702.5 28 27922.0 29 34179.5 30 43416.5 31 53049.0 32 64768.0 33 79056.0 34 93329.0 35 107543.5 36 124926.5 37 142970.5 38 151210.0 39 152744.5 40 151991.5 41 150367.0 42 152746.5 43 160208.0 44 170020.0 45 184081.5 46 196118.5 47 203824.5 48 215431.5 49 227996.5 50 236496.5 51 237093.0 52 230076.5 53 220299.0 54 209519.5 55 192515.0 56 172632.5 57 153236.0 58 133454.5 59 112566.0 60 91015.0 61 73036.5 62 58885.5 63 46920.5 64 36034.5 65 27009.5 66 20061.0 67 15515.0 68 11834.0 69 8568.5 70 6630.0 71 5168.5 72 4122.5 73 3316.0 74 2489.5 75 1817.0 76 1271.5 77 780.5 78 494.5 79 324.5 80 207.0 81 123.5 82 76.5 83 45.0 84 35.5 85 22.5 86 7.0 87 5.0 88 4.0 89 1.5 90 2.0 91 2.0 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029050516892487988 2 0.0015872582291956588 3 7.382596414863528E-5 4 0.021594094513475822 5 5.721512221519235E-4 6 5.5369473111476466E-5 7 0.0 8 0.003506733297060176 9 0.010483286909106212 10-11 0.0010151070070437353 12-13 9.228245518579411E-5 14-15 1.3842368277869118E-4 16-17 3.1376034763169996E-4 18-19 5.5369473111476466E-5 20-21 5.5369473111476466E-5 22-23 3.414450841874382E-4 24-25 1.845649103715882E-5 26-27 8.305420966721471E-5 28-29 0.0010612482346366323 30-31 0.002713104182462347 32-33 0.009745027267619858 34-35 0.018770251384790524 36-37 0.021520268549327187 38-39 0.021169595219621168 40-41 0.004558753286178229 42-43 0.004577209777215388 44-45 0.008517670613648796 46-47 0.011378426724408414 48-49 1.20391691035387 50-51 0.017026112981779015 52-53 0.018742566648234784 54-55 0.009984961651102922 56-57 0.008047030092201247 58-59 0.008213138511535677 60-61 0.005361610646294638 62-63 0.007410281151419266 64-65 0.018761023139271943 66-67 0.02839531146066885 68-69 0.028496822161373222 70-71 0.02519311026572179 72-73 0.023089070287485688 74-75 0.008923713416466292 76-77 0.014211498098612295 78-79 0.008342333948795787 80-81 0.0025100827810535997 82-83 0.013260988810198613 84-85 0.023153668006115746 86-87 0.02224929994529496 88-89 0.031071502661056877 90-91 0.02020985768568891 92-93 0.013067195654308446 94-95 0.00643208712644985 96-97 0.003774352417098979 98-99 0.015558821944324888 100 0.034255247364966775 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5418148.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.51183717880204 #Duplication Level Percentage of deduplicated Percentage of total 1 77.11505965698481 45.89258874340086 2 14.062070656709267 16.737193186377837 3 4.178591093640611 7.460268984045972 4 1.6504100225639164 3.928757301643472 5 0.8741853349970399 2.601218766022018 6 0.521490546542199 1.8620916297662298 7 0.3217274879427137 1.3402615714874242 8 0.22040923947146793 1.049356701770367 9 0.16670731840010491 0.8928952910227578 >10 0.7949253966290414 8.261829033125165 >50 0.05252118496161665 2.164819158747907 >100 0.038016079900866084 4.442233784360364 >500 0.0028856297713226167 1.208057134741171 >1k 8.464512566612534E-4 0.9399764023734747 >5k 3.847505712095374E-5 0.12680476752273628 >10k+ 1.1542517136286121E-4 1.091647543592267 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20159 0.37206440281808467 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16954 0.31291134904399065 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10563 0.19495591482550864 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5538 0.10221204736378556 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.845649103715882E-5 2 0.0 0.0 0.0 0.0 1.845649103715882E-5 3 0.0 0.0 0.0 0.0 1.845649103715882E-5 4 0.0 0.0 0.0 0.0 3.691298207431764E-5 5 0.0 0.0 0.0 0.0 3.691298207431764E-5 6 3.691298207431764E-5 0.0 0.0 0.0 3.691298207431764E-5 7 3.691298207431764E-5 0.0 0.0 0.0 3.691298207431764E-5 8 3.691298207431764E-5 0.0 0.0 0.0 3.691298207431764E-5 9 3.691298207431764E-5 0.0 0.0 0.0 3.691298207431764E-5 10-11 5.5369473111476466E-5 0.0 0.0 0.0 3.691298207431764E-5 12-13 5.5369473111476466E-5 0.0 0.0 0.0 3.691298207431764E-5 14-15 5.5369473111476466E-5 0.0 0.0 0.0 1.0151070070437353E-4 16-17 5.5369473111476466E-5 0.0 0.0 0.0 1.2919543726011177E-4 18-19 5.5369473111476466E-5 0.0 0.0 0.0 1.2919543726011177E-4 20-21 5.5369473111476466E-5 0.0 0.0 4.6141227592897054E-5 1.3842368277869116E-4 22-23 7.382596414863528E-5 0.0 0.0 2.768473655573823E-4 1.753366648530088E-4 24-25 8.30542096672147E-5 0.0 0.0 9.966505160065764E-4 2.2147789244590587E-4 26-27 9.228245518579411E-5 0.0 0.0 0.002805386637648141 2.399343834830647E-4 28-29 9.228245518579411E-5 0.0 0.0 0.007336455187270632 2.399343834830647E-4 30-31 1.0151070070437352E-4 0.0 0.0 0.022480006083259445 2.583908745202235E-4 32-33 1.1073894622295293E-4 0.0 0.0 0.05910691254650113 2.768473655573823E-4 34-35 1.1996719174153236E-4 0.0 0.0 0.1147901460056093 2.9530385659454114E-4 36-37 1.2919543726011177E-4 0.0 0.0 0.19438376360335674 2.9530385659454114E-4 38-39 1.2919543726011177E-4 0.0 0.0 0.3190758170504017 3.1376034763169996E-4 40-41 1.8456491037158822E-4 0.0 0.0 0.47268919195267456 3.4144508418743817E-4 42-43 1.8456491037158822E-4 0.0 0.0 0.6212916295383588 3.5990157522459705E-4 44-45 1.8456491037158822E-4 0.0 0.0 0.7876307550107527 3.6912982074317643E-4 46-47 2.0302140140874704E-4 0.0 0.0 0.9683013457735005 4.1527104833607347E-4 48-49 2.0302140140874704E-4 9.22824551857941E-6 0.0 1.1649368012833905 4.798687669661294E-4 50-51 2.0302140140874704E-4 1.845649103715882E-5 0.0 1.393880344353827 4.798687669661294E-4 52-53 2.0302140140874704E-4 1.845649103715882E-5 0.0 1.6344330202866368 4.890970124847088E-4 54-55 2.1224964692732645E-4 1.845649103715882E-5 0.0 1.9076536853552173 4.983252580032882E-4 56-57 2.2147789244590587E-4 1.845649103715882E-5 0.0 2.1959994448287494 5.075535035218676E-4 58-59 2.2147789244590587E-4 1.845649103715882E-5 0.0 2.5314830824111856 5.260099945590265E-4 60-61 2.3070613796448528E-4 1.845649103715882E-5 0.0 2.8582829409606383 5.352382400776059E-4 62-63 3.045321021131205E-4 1.845649103715882E-5 0.0 3.2047851036922577 5.352382400776059E-4 64-65 3.1376034763169996E-4 1.845649103715882E-5 0.0 3.542714226337117 5.352382400776059E-4 66-67 3.322168386688588E-4 1.845649103715882E-5 0.0 3.912849925841819 5.352382400776059E-4 68-69 3.6912982074317643E-4 1.845649103715882E-5 0.0 4.335946526377648 5.352382400776059E-4 70-71 3.6912982074317643E-4 1.845649103715882E-5 0.0 4.769960141361956 5.536947311147646E-4 72-73 3.6912982074317643E-4 1.845649103715882E-5 0.0 5.170309116694487 6.459771863005588E-4 74-75 3.6912982074317643E-4 1.845649103715882E-5 0.0 5.584832677143556 6.552054318191382E-4 76-77 3.6912982074317643E-4 2.768473655573823E-5 0.0 6.0443070215136245 6.73661922856297E-4 78-79 4.060428028174941E-4 3.691298207431764E-5 0.0 6.526058350565544 6.828901683748764E-4 80-81 4.060428028174941E-4 3.691298207431764E-5 0.0 7.000953093197159 7.105749049306147E-4 82-83 4.060428028174941E-4 3.691298207431764E-5 0.0 7.484725408017647 7.659443780420911E-4 84-85 4.060428028174941E-4 3.691298207431764E-5 0.0 7.99048863190891 7.751726235606705E-4 86-87 4.060428028174941E-4 3.691298207431764E-5 0.0 8.526889630921858 7.8440086907925E-4 88 4.060428028174941E-4 3.691298207431764E-5 0.0 8.938773913152612 7.936291145978294E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10445 0.0 51.395 1 TACCTGG 5245 0.0 44.632797 2 GTATCAA 18040 0.0 39.823734 1 ACCTGGG 6280 0.0 36.832096 3 GTACCTG 7170 0.0 33.246647 1 ATCAACG 22800 0.0 30.908236 3 CAACGCA 23070 0.0 30.663427 5 TATCAAC 23530 0.0 30.126007 2 TCAACGC 23745 0.0 29.796707 4 AACGCAG 24600 0.0 29.137697 6 GTACATG 26455 0.0 28.62853 1 TACATGG 27300 0.0 27.477985 2 TGGGGGG 13145 0.0 26.979145 6 ACATGGG 27260 0.0 26.608646 3 GTGGTAT 4985 0.0 26.451143 1 ATGGGGG 14210 0.0 26.079542 5 CATGGGG 19185 0.0 26.06961 4 CCTGGGG 6160 0.0 25.896278 4 ACGCAGA 28045 0.0 25.290842 7 CGCAGAG 28570 0.0 24.809683 8 >>END_MODULE