FastQCFastQC Report
Mon 6 Jun 2016
ERR1211361_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211361_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11673671
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT429570.367981931305071No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328360.28128255456231377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195910.1678221015480049No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA197100.051.6396031
GTATCAA374550.043.1223831
ATCAACG460900.034.2864653
CAACGCA461200.034.2331545
TATCAAC480800.033.200082
AACGCAG493450.032.337936
TCAACGC492550.032.178574
GTACTAG50050.028.6621021
ACGCAGA556200.028.3691737
CGCAGAG571150.027.5353978
GTACATG526850.027.389281
TACATGG540700.026.3298152
ACATGGG541550.025.4493143
GCAGAGT619050.024.792119
GTGGTAT90300.024.7410491
GCATCGT52350.023.426628
CATGGGG348200.022.9819344
CTGTGCG67050.022.2169049
GAGTACT339850.022.1858212-13
TAGTACT70050.021.7338964
Produced by FastQC (version 0.11.5)