FastQCFastQC Report
Mon 6 Jun 2016
ERR1211361_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211361_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11673671
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377730.32357430665983306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308330.26412428446887015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192680.16505519129329582No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA205100.050.8381231
GTATCAA372050.041.1554071
CAACGCA464950.032.1460655
ATCAACG466250.032.0416683
TATCAAC486650.031.2453922
TCAACGC493100.030.401624
AACGCAG501500.030.0464326
GTACTAG50650.028.3500021
GTGGTAT96700.026.8355061
GTACATG528950.026.277351
ACGCAGA566950.026.2633727
TACATGG538100.025.6422252
CGCAGAG582050.025.5336678
ACATGGG535050.024.957523
TGGTATC101850.024.460042
GCAGAGT633500.023.1119
CATGGGG343850.022.4606174
CTGTGCG68850.022.1437429
CTAGTAC70500.021.4968243
TAGTACT73500.021.3854484
Produced by FastQC (version 0.11.5)