##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211361_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11673671 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0674537598327 33.0 31.0 34.0 30.0 34.0 2 32.20351901299942 33.0 31.0 34.0 30.0 34.0 3 32.2678197800846 34.0 31.0 34.0 30.0 34.0 4 35.914644673470754 37.0 35.0 37.0 35.0 37.0 5 35.8188247724302 37.0 35.0 37.0 35.0 37.0 6 35.8371039409968 37.0 35.0 37.0 35.0 37.0 7 35.75011279656588 37.0 35.0 37.0 33.0 37.0 8 35.73499887053524 37.0 35.0 37.0 33.0 37.0 9 37.34063757664577 39.0 37.0 39.0 34.0 39.0 10-11 37.2917530398107 39.0 37.0 39.0 34.0 39.0 12-13 37.2182801793883 39.0 37.0 39.0 33.0 39.0 14-15 38.43584961405885 40.0 38.0 41.0 33.5 41.0 16-17 38.4236574767269 40.0 38.0 41.0 34.0 41.0 18-19 38.437229813997675 40.0 38.0 41.0 34.0 41.0 20-21 38.40884975257569 40.0 38.0 41.0 34.0 41.0 22-23 38.29714251840745 40.0 38.0 41.0 34.0 41.0 24-25 38.20562447751011 40.0 38.0 41.0 33.0 41.0 26-27 38.1034014492956 40.0 37.5 41.0 33.0 41.0 28-29 38.001691113275335 40.0 37.0 41.0 33.0 41.0 30-31 38.007038659903984 40.0 37.0 41.0 33.0 41.0 32-33 37.93880018547722 40.0 37.0 41.0 33.0 41.0 34-35 37.80640520021508 40.0 37.0 41.0 33.0 41.0 36-37 37.6594143778765 40.0 37.0 41.0 33.0 41.0 38-39 37.53998185318055 40.0 36.5 41.0 32.0 41.0 40-41 37.423978626774726 40.0 36.0 41.0 32.0 41.0 42-43 37.31076848062619 40.0 36.0 41.0 31.5 41.0 44-45 37.13850917162219 39.0 36.0 41.0 31.0 41.0 46-47 36.878479314690296 39.0 35.5 41.0 30.5 41.0 48-49 35.32307133719976 39.0 35.0 40.0 24.0 41.0 50-51 36.037947446009056 38.5 35.0 40.0 28.0 41.0 52-53 36.1244987545049 39.0 35.0 40.0 29.0 41.0 54-55 35.95287069508812 38.0 35.0 40.0 28.5 41.0 56-57 35.73044139242917 38.0 34.5 40.0 28.0 41.0 58-59 35.48858936490501 38.0 34.0 40.0 28.0 41.0 60-61 35.23562395239681 37.5 34.0 40.0 27.5 41.0 62-63 34.91691431084532 37.0 34.0 40.0 27.0 41.0 64-65 34.58637175914929 36.0 33.5 39.0 26.0 41.0 66-67 34.250199273219195 36.0 33.0 39.0 26.0 40.0 68-69 33.89564212491512 35.5 33.0 39.0 26.0 40.0 70-71 33.5493432185985 35.0 32.5 38.0 26.0 40.0 72-73 33.18192790425566 35.0 32.0 37.0 25.0 39.5 74-75 32.82448871481816 35.0 32.0 37.0 25.0 39.0 76-77 31.901008131889274 34.0 30.5 36.0 23.5 38.5 78-79 32.346126895301396 34.5 31.5 36.0 25.0 38.0 80-81 32.2238054336121 35.0 31.5 35.5 25.0 37.0 82-83 31.960902787135254 34.0 31.0 35.0 24.0 37.0 84-85 31.682372622973528 34.0 31.0 35.0 24.0 36.5 86-87 31.42600699471486 34.0 31.0 35.0 23.5 36.0 88-89 31.20035921005483 34.0 31.0 35.0 23.0 36.0 90-91 30.976500836797612 34.0 30.5 35.0 21.5 35.0 92-93 30.78354456794268 34.0 30.0 35.0 20.0 35.0 94-95 30.589820502907784 34.0 30.0 35.0 20.0 35.0 96-97 30.401178643804506 34.0 29.5 35.0 20.0 35.0 98-99 30.186387812368537 34.0 29.0 35.0 19.0 35.0 100 27.716227740185584 31.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3320295928478991 1101 2 -0.2736186640468752 1101 3 -0.28294843074132814 1101 4 -0.21740998489546826 1101 5 -0.19399559878193173 1101 6 -0.23402999895771615 1101 7 -0.22875658269119015 1101 8 -0.23695725065340412 1101 9 -0.356109866087408 1101 10-11 -0.3332259077253923 1101 12-13 -0.437633252433109 1101 14-15 -0.5924513055850156 1101 16-17 -0.5788785332859945 1101 18-19 -0.5389926768971094 1101 20-21 -0.5465031887632037 1101 22-23 -0.6328926472933887 1101 24-25 -0.6076497350801375 1101 26-27 -0.7018437729340121 1101 28-29 -0.5541195158537633 1101 30-31 -0.6128634961274457 1101 32-33 -0.539016587321818 1101 34-35 -0.6189804781074102 1101 36-37 -0.6064941785576323 1101 38-39 -0.6147385043201368 1101 40-41 -0.6068295421286365 1101 42-43 -0.6473355025818037 1101 44-45 -0.630036464483652 1101 46-47 -0.6082761858201096 1101 48-49 0.5568173555649736 1101 50-51 -0.31980206332335825 1101 52-53 -0.6093173764407211 1101 54-55 -0.7036841391746194 1101 56-57 -0.7208376524447289 1101 58-59 -0.7876480362046223 1101 60-61 -0.7902219803787887 1101 62-63 -0.8267844622245377 1101 64-65 -0.8213779382425841 1101 66-67 -0.7634444276496168 1101 68-69 -0.8213105693672205 1101 70-71 -0.7204919737522246 1101 72-73 -0.8908499544925377 1101 74-75 -0.8782941847055596 1101 76-77 -0.8655401456272358 1101 78-79 -0.7922494064309618 1101 80-81 -0.6409899786459547 1101 82-83 -0.696876243727985 1101 84-85 -0.738335981718631 1101 86-87 -0.7117475726849207 1101 88-89 -0.7731470715013877 1101 90-91 -0.8108636378928615 1101 92-93 -0.8156766212210904 1101 94-95 -0.8348990244282177 1101 96-97 -0.9334712657941147 1101 98-99 -1.0532516109998227 1101 100 -0.9327639138493709 1102 1 -0.3210095342804067 1102 2 -0.31055157294850133 1102 3 -0.33043155032652294 1102 4 -0.22936985748891914 1102 5 -0.2290441577545934 1102 6 -0.3226967895588757 1102 7 -0.28061988098870216 1102 8 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1102 72-73 -0.839011988769748 1102 74-75 -0.9332686129115899 1102 76-77 -0.8281494891795518 1102 78-79 -0.7127990567400673 1102 80-81 -0.6469830584859508 1102 82-83 -0.7271084247577555 1102 84-85 -0.6232902729351508 1102 86-87 -0.6836328832075509 1102 88-89 -0.7542944874554429 1102 90-91 -0.7255338712914465 1102 92-93 -0.8266814329405108 1102 94-95 -0.8884030905303071 1102 96-97 -0.9691327016008628 1102 98-99 -1.1080746603890361 1102 100 -0.9628921502315038 1103 1 -0.35821252446979557 1103 2 -0.33357521794139444 1103 3 -0.34520653485313346 1103 4 -0.19915050065808515 1103 5 -0.19525158525711106 1103 6 -0.25402750792389384 1103 7 -0.2950281909913386 1103 8 -0.34025861829802295 1103 9 -0.4661772787232934 1103 10-11 -0.47740084689362305 1103 12-13 -0.5053275994882753 1103 14-15 -0.6126927991944555 1103 16-17 -0.5739038536391377 1103 18-19 -0.5522748125228105 1103 20-21 -0.6543443108321085 1103 22-23 -0.6862008356258613 1103 24-25 -0.763264669730475 1103 26-27 -0.7184965661410629 1103 28-29 -0.726300147782112 1103 30-31 -0.7189058261672514 1103 32-33 -0.7269172672760718 1103 34-35 -0.7263384143967713 1103 36-37 -0.7108605405239317 1103 38-39 -0.6800583236660316 1103 40-41 -0.7334158742437893 1103 42-43 -0.7716769903117822 1103 44-45 -0.6850682367691263 1103 46-47 -0.823734095084582 1103 48-49 0.4896831043140466 1103 50-51 -0.4353345359864491 1103 52-53 -0.8055186143246473 1103 54-55 -0.887411744367931 1103 56-57 -0.9112983853966199 1103 58-59 -0.9623189154518812 1103 60-61 -0.9941618623976538 1103 62-63 -1.024390142916296 1103 64-65 -1.007978213442513 1103 66-67 -0.9507756768695614 1103 68-69 -1.0399028619125872 1103 70-71 -1.0174702800967808 1103 72-73 -0.9709253198699557 1103 74-75 -1.1238931519837934 1103 76-77 -1.0461767285477137 1103 78-79 -0.7235739633828047 1103 80-81 -0.8450041532988841 1103 82-83 -0.897619660023576 1103 84-85 -0.920463432911383 1103 86-87 -0.8451979665570839 1103 88-89 -0.7918978386676052 1103 90-91 -0.8968523591312234 1103 92-93 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1105 70-71 -0.8666928015019124 1105 72-73 -0.7946648133559719 1105 74-75 -0.8914775747063786 1105 76-77 -0.8354806484572705 1105 78-79 -0.7276507633902369 1105 80-81 -0.677535230429605 1105 82-83 -0.6799977548813558 1105 84-85 -0.6554091122090853 1105 86-87 -0.592676209727955 1105 88-89 -0.7076752759879525 1105 90-91 -0.7498040158386949 1105 92-93 -0.6757111557975151 1105 94-95 -0.7693077993812558 1105 96-97 -0.7993901708814164 1105 98-99 -0.8419200565983118 1105 100 -0.8820879461461999 1106 1 -0.3282911550030363 1106 2 -0.2964668838885416 1106 3 -0.32015343774428473 1106 4 -0.2555884882629229 1106 5 -0.26782769573654974 1106 6 -0.31060817721272826 1106 7 -0.3862932181233063 1106 8 -0.3837480371928592 1106 9 -0.49059221360066374 1106 10-11 -0.4293776847644395 1106 12-13 -0.5059366818419377 1106 14-15 -0.6268938571836031 1106 16-17 -0.6021849645572246 1106 18-19 -0.6419237197019569 1106 20-21 -0.6537308593449254 1106 22-23 -0.6250749454582518 1106 24-25 -0.5599581025413514 1106 26-27 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0.4416143871189462 2311 10-11 0.3708388197870889 2311 12-13 0.5122606395927036 2311 14-15 0.47372675488870186 2311 16-17 0.4848180948268137 2311 18-19 0.6172522367277935 2311 20-21 0.6486979574125158 2311 22-23 0.7017680030444282 2311 24-25 0.6384244083408177 2311 26-27 0.6677902522366566 2311 28-29 0.7448151597621191 2311 30-31 0.6046146067652884 2311 32-33 0.6430383063546685 2311 34-35 0.7735327031797326 2311 36-37 0.8023274083027658 2311 38-39 0.8448243967696527 2311 40-41 0.7781612952005474 2311 42-43 0.7916122765308913 2311 44-45 0.824344212650729 2311 46-47 0.8838882951861322 2311 48-49 -5.774933275840496 2311 50-51 -1.0203037900226164 2311 52-53 0.4395939796488264 2311 54-55 0.8776232419982577 2311 56-57 0.9177847385332427 2311 58-59 0.9967956914043938 2311 60-61 0.8535555107350987 2311 62-63 0.7351424930554487 2311 64-65 0.7681650821910253 2311 66-67 0.9453581120901688 2311 68-69 0.9537332225278377 2311 70-71 0.9562250344844401 2311 72-73 0.43315885071881866 2311 74-75 0.7542172673765819 2311 76-77 0.8738915473068403 2311 78-79 0.7499199338788713 2311 80-81 0.8024795639862106 2311 82-83 0.8395982331409364 2311 84-85 0.8763221843893945 2311 86-87 0.8910857010905744 2311 88-89 0.9503480139106308 2311 90-91 0.9779735334609292 2311 92-93 0.9571200616854973 2311 94-95 0.9903222872072952 2311 96-97 1.0493477344843178 2311 98-99 1.0707359576455246 2311 100 1.297742794874889 2312 1 0.06451939602084877 2312 2 0.23462725338426083 2312 3 0.1795632660954425 2312 4 0.005505244125743047 2312 5 0.15171173071004063 2312 6 0.1343833334406952 2312 7 0.2909675356971704 2312 8 0.3097739379689912 2312 9 0.27429623976795625 2312 10-11 0.2610469022128896 2312 12-13 0.2846376617690325 2312 14-15 0.3381256859276789 2312 16-17 0.46262953731665846 2312 18-19 0.3681091485719534 2312 20-21 0.4043826873495604 2312 22-23 0.6080070817461944 2312 24-25 0.6976081162660464 2312 26-27 0.6610116256966307 2312 28-29 0.7287455451868965 2312 30-31 0.7761357196014629 2312 32-33 0.7742946238047068 2312 34-35 0.36793348419677585 2312 36-37 0.7012243209679525 2312 38-39 0.7639819041058402 2312 40-41 0.5510496263269076 2312 42-43 0.5930576321362437 2312 44-45 0.7262652771486415 2312 46-47 0.7598495775006526 2312 48-49 -4.625506220663318 2312 50-51 -0.7652718534692085 2312 52-53 0.5389171047092347 2312 54-55 0.7974859530599403 2312 56-57 0.8771981732853789 2312 58-59 0.9160631841904419 2312 60-61 0.9083736891877905 2312 62-63 0.6778468450034154 2312 64-65 0.9158120126979981 2312 66-67 0.897624742943151 2312 68-69 0.8300073168362161 2312 70-71 0.8568452970910769 2312 72-73 0.9474788797324507 2312 74-75 0.608723446974345 2312 76-77 0.7808951302973881 2312 78-79 0.4677507383549795 2312 80-81 0.6622142732929035 2312 82-83 0.6610320863683476 2312 84-85 0.689463157316851 2312 86-87 0.8072072712994327 2312 88-89 0.8555769617887243 2312 90-91 0.7153286532878234 2312 92-93 0.8727226758424713 2312 94-95 1.027453510796196 2312 96-97 1.0645805844432878 2312 98-99 1.0834635074263446 2312 100 0.7462207609065743 2313 1 0.11879898423789115 2313 2 0.26119290599560685 2313 3 0.34099061722937307 2313 4 0.1311098104252295 2313 5 0.1768699942265215 2313 6 0.2785722298311626 2313 7 0.19003066600748042 2313 8 0.26627604280404427 2313 9 0.4918917975563346 2313 10-11 0.27999346705247063 2313 12-13 0.06893865821712808 2313 14-15 0.1972950540914553 2313 16-17 0.1466381849184657 2313 18-19 0.46247798864108347 2313 20-21 0.4311604869313115 2313 22-23 0.5006926670488454 2313 24-25 0.3380086323145548 2313 26-27 0.501564920573145 2313 28-29 0.4562691917485182 2313 30-31 0.6083546252048109 2313 32-33 0.5241006715189158 2313 34-35 0.4545400614526045 2313 36-37 -0.2682437180026085 2313 38-39 0.1824532180178906 2313 40-41 0.4486936842826168 2313 42-43 0.5292818287923069 2313 44-45 0.5637451286126449 2313 46-47 0.5504437276116718 2313 48-49 -5.230489474601168 2313 50-51 -1.221433835269302 2313 52-53 0.17206814113193758 2313 54-55 0.3794943423752244 2313 56-57 0.40776646433512553 2313 58-59 0.5519035690898093 2313 60-61 0.826150900469095 2313 62-63 0.8424224785438597 2313 64-65 0.9549398232751329 2313 66-67 0.9687825379309629 2313 68-69 0.9364161178137351 2313 70-71 0.9380825064793186 2313 72-73 0.6908217249838771 2313 74-75 0.8555481466764192 2313 76-77 0.8586610465174402 2313 78-79 0.7928164085053382 2313 80-81 -0.10255191155734167 2313 82-83 0.328310828857564 2313 84-85 0.6923810500239682 2313 86-87 0.8172165607061643 2313 88-89 0.8237646805382646 2313 90-91 0.8640176225418124 2313 92-93 0.9193230416963765 2313 94-95 0.7881865320777841 2313 96-97 0.8416707345893961 2313 98-99 1.0677201962282012 2313 100 1.1023087187712335 2314 1 0.06858766451867382 2314 2 0.1989908418951103 2314 3 0.22401771013629457 2314 4 0.08879123117843335 2314 5 0.2199807704874317 2314 6 0.31537592339799403 2314 7 0.14212723725948706 2314 8 0.239341126436301 2314 9 0.4517115379824972 2314 10-11 0.3184751811002897 2314 12-13 0.23238972904815824 2314 14-15 0.24076995924973232 2314 16-17 0.4521950314601426 2314 18-19 0.5306872290120026 2314 20-21 0.6150078745931395 2314 22-23 0.31430577897054945 2314 24-25 0.43622283029003484 2314 26-27 0.5015101272005325 2314 28-29 0.584575404459585 2314 30-31 0.5311541350179283 2314 32-33 0.47962405918674733 2314 34-35 0.37361778358833675 2314 36-37 0.4294600959669239 2314 38-39 0.46581520252740205 2314 40-41 0.27572223908696003 2314 42-43 0.3203011095010382 2314 44-45 0.5339848809664289 2314 46-47 0.6125837426707861 2314 48-49 -6.447650546460853 2314 50-51 -1.570075398672877 2314 52-53 -0.9310788330722417 2314 54-55 -0.4607334668862748 2314 56-57 -0.14018458382467003 2314 58-59 0.374698544654656 2314 60-61 0.5607667255533499 2314 62-63 0.5129102462162081 2314 64-65 0.6275968561567566 2314 66-67 0.659980076041137 2314 68-69 0.6702235241258947 2314 70-71 0.4760911313124154 2314 72-73 0.6661600989806615 2314 74-75 0.7071158036416492 2314 76-77 0.6747009399711033 2314 78-79 0.4033900935368422 2314 80-81 0.4881383049620638 2314 82-83 0.5294419876212082 2314 84-85 0.5573757415108602 2314 86-87 0.6073238278162449 2314 88-89 0.49488877907861095 2314 90-91 0.7066403799055614 2314 92-93 0.7117546067404277 2314 94-95 0.886491088186748 2314 96-97 0.8804639031223083 2314 98-99 0.956294035480802 2314 100 -0.41940005702988614 2315 1 0.20207353734545563 2315 2 0.27490747965040185 2315 3 0.3267433386986198 2315 4 0.15727648313553289 2315 5 0.27072809332806713 2315 6 0.30794662706508547 2315 7 0.34968635974547624 2315 8 0.256565025774222 2315 9 0.45335558469509607 2315 10-11 0.2519338887464002 2315 12-13 0.4317781521568591 2315 14-15 0.27652800723033977 2315 16-17 0.4344274287386085 2315 18-19 0.5246154028329926 2315 20-21 0.4310453926051778 2315 22-23 0.48542299068062533 2315 24-25 0.5388569754531147 2315 26-27 0.6183400727857418 2315 28-29 0.6682908807365635 2315 30-31 0.662686245877623 2315 32-33 0.7145262280772897 2315 34-35 0.7588932834225446 2315 36-37 0.6697168554297264 2315 38-39 0.7404857428921261 2315 40-41 0.7255446686523612 2315 42-43 0.7551451059726517 2315 44-45 0.7931268512196041 2315 46-47 0.7123098905291911 2315 48-49 -4.695437978634253 2315 50-51 -0.7814255787250843 2315 52-53 0.37450302735864227 2315 54-55 0.34218972488195476 2315 56-57 0.6790354882951419 2315 58-59 0.8104150794376821 2315 60-61 0.6570308639840974 2315 62-63 0.41468474485541407 2315 64-65 0.6321427437138496 2315 66-67 -0.0763916745418598 2315 68-69 0.6393001203409057 2315 70-71 0.6134964145722677 2315 72-73 0.7810873785531598 2315 74-75 0.8650738828704192 2315 76-77 0.8432165222223489 2315 78-79 0.7599429988402306 2315 80-81 0.622432086594003 2315 82-83 0.5846659631606421 2315 84-85 0.6025595006004636 2315 86-87 0.6597422111705242 2315 88-89 0.6103419962496126 2315 90-91 0.7069487999047936 2315 92-93 0.803156975238867 2315 94-95 0.3834832176401264 2315 96-97 0.5268119165269525 2315 98-99 0.47561454798313463 2315 100 0.6334936370321422 2316 1 0.25637325655870313 2316 2 0.2932840650976587 2316 3 0.17283084110732716 2316 4 0.019679790638321037 2316 5 0.19671749242376535 2316 6 0.1565138532980015 2316 7 0.2758573368558501 2316 8 0.3422139537262723 2316 9 0.4507170580685269 2316 10-11 0.4278181218475865 2316 12-13 0.3854903719722884 2316 14-15 0.5240020125191762 2316 16-17 0.5019844183846018 2316 18-19 0.1788518719051595 2316 20-21 0.48183710543878533 2316 22-23 0.5787136260466781 2316 24-25 0.6129502144625221 2316 26-27 0.6771414342229889 2316 28-29 0.732142607867587 2316 30-31 0.6795977012717529 2316 32-33 0.6478851933815477 2316 34-35 0.6164205448570357 2316 36-37 0.5269444905583569 2316 38-39 0.6438988202942966 2316 40-41 0.26028127622534214 2316 42-43 0.2654615781933458 2316 44-45 0.5044412175396502 2316 46-47 0.5075476560034318 2316 48-49 -4.829101473689512 2316 50-51 -0.8316712671343751 2316 52-53 0.4050184031001791 2316 54-55 0.6626336931282779 2316 56-57 0.5817777399915656 2316 58-59 0.42696997049517194 2316 60-61 0.5728495500672537 2316 62-63 0.4625426812964406 2316 64-65 0.5323367738984217 2316 66-67 0.5903186428228722 2316 68-69 0.3070726142503091 2316 70-71 -0.08449334496966543 2316 72-73 0.08962086779310852 2316 74-75 0.33010824354719404 2316 76-77 0.35941194558752443 2316 78-79 0.3984981105527581 2316 80-81 0.33640563637860055 2316 82-83 0.33787398837983673 2316 84-85 0.40902295100856634 2316 86-87 0.4154540580660395 2316 88-89 0.29117659057125067 2316 90-91 0.3177392825758467 2316 92-93 0.42408623401000867 2316 94-95 0.6497420524439192 2316 96-97 0.7325670771879018 2316 98-99 0.6963316359733085 2316 100 -0.022824946680849223 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 12.0 11 17.0 12 65.0 13 284.0 14 971.0 15 2803.0 16 7048.0 17 14049.0 18 24882.0 19 37469.0 20 54486.0 21 76698.0 22 94025.0 23 97074.0 24 99000.0 25 108265.0 26 128460.0 27 156832.0 28 193130.0 29 238662.0 30 293368.0 31 363221.0 32 458276.0 33 596427.0 34 823314.0 35 1158351.0 36 1791180.0 37 2574899.0 38 2019754.0 39 260631.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.23797070502285 20.09080346110541 14.083370893193466 23.58785494067827 2 15.451560202987139 20.41984335563551 40.3663732972149 23.76222314416245 3 18.8833285062256 25.740699765292057 29.59853112011086 25.77744060837148 4 12.644969216667127 16.664093320444326 38.12790861667703 32.563028846211516 5 13.287730763000411 38.01020952300021 34.52484411207466 14.177215601924717 6 31.237729857177637 37.24077328947848 17.45493402156067 14.066562831783205 7 27.887915958555958 32.21926199894823 21.873200276622544 18.019621765873264 8 26.21237203201307 35.592002283514965 19.666421433241453 18.529204251230507 9 26.37391756246178 15.891397025726627 20.576263966804706 37.15842144500689 10-11 25.32275311942356 26.34730426071724 27.433237752591307 20.896704867267896 12-13 25.609514308687864 23.874634171058357 28.14396805046438 22.3718834697894 14-15 22.92096275420863 25.36171941700005 25.803697548184246 25.91362028060707 16-17 22.050052565066 28.345032424582676 26.718759871342478 22.886155139008846 18-19 21.76430470298992 27.432715137258256 29.09999722023996 21.702982939511863 20-21 22.77863562037439 26.574371740767432 28.991213513117497 21.65577912574068 22-23 22.753861009152935 26.358052061260796 28.705504850589776 22.182582078996493 24-25 22.35000173039014 26.520798775500552 29.093255007766196 22.03594448634311 26-27 22.246720866168644 26.755485982033743 28.737314820319448 22.260478331478158 28-29 21.95780390907854 26.80725891082415 28.999013540802583 22.235923639294732 30-31 22.447122846531656 26.65198111463537 28.90133001775493 21.999566021078046 32-33 22.37842355253793 26.907261207230558 28.5195149214492 22.194800318782303 34-35 22.51226981065812 26.78801062379723 28.84060731353799 21.859112252006657 36-37 22.448667992865346 26.621138653022257 28.61203462111124 22.318158733001162 38-39 22.40567771546546 26.71726593937923 28.848084285968145 22.028972059187165 40-41 22.355605259369398 26.375377645542674 29.057871575950568 22.21114551913736 42-43 22.197118433210594 26.422232186256707 28.765068627049523 22.61558075348318 44-45 22.2024357023656 26.874751100853324 28.581343078964206 22.34147011781687 46-47 22.322846877659742 26.746148502475155 28.226476668865175 22.704527950999935 48-49 22.67247530994972 26.850782174012807 28.04169450321712 22.435048012820356 50-51 22.47361597244936 26.85144856991513 27.92780080019038 22.747134657445137 52-53 22.296269415450947 27.073961262423442 27.854612617165923 22.775156704959688 54-55 22.249831035944773 27.04010014563164 27.842654777628397 22.867414040795193 56-57 22.523726987495014 26.921786610632047 27.59150259269768 22.96298380917526 58-59 22.151245476754898 26.967004972860316 27.975061422924945 22.90668812745984 60-61 22.29898800635341 27.270170889524863 27.65813134252204 22.772709761599685 62-63 22.31788469803889 27.054126036993146 27.644223829204467 22.9837654357635 64-65 21.987865005479396 26.877796647657746 27.774081912605304 23.360256434257558 66-67 22.19516116890897 26.81839831535018 27.653966770487028 23.332473745253825 68-69 22.02168265948067 26.939682365314976 27.70133416096176 23.33730081424259 70-71 22.063799380847506 26.597243396575703 27.823989457981725 23.514967764595067 72-73 22.212781938105085 26.88962112020424 27.63773031559743 23.25986662609325 74-75 22.212029317356897 27.038945668309328 27.462844098887075 23.2861809154467 76-77 22.051795136113963 26.937057144697935 27.46035292714589 23.55079479204221 78-79 22.022886714528266 26.832377053762247 27.51813635761808 23.626599874091415 80-81 21.914110009005547 27.20226926903024 27.398510926050655 23.48510979591356 82-83 22.257663890753264 26.741053046785 27.81345885915391 23.187824203307827 84-85 22.271958794041623 26.843962395981436 27.31946420566958 23.56461460430736 86-87 21.777924123508598 27.14058263936925 27.62798760996601 23.453505627156147 88-89 22.025724864109534 26.636540385130026 27.558426708353483 23.77930804240696 90-91 22.054358830416092 26.639655099142374 27.61121387033625 23.694772200105284 92-93 22.105100151835746 26.764429268723145 27.464145966221743 23.66632461321937 94-95 21.649169064878553 26.55119754392491 27.774279180733753 24.025354210462783 96-97 21.787578605435183 26.504819200163933 27.689094759306414 24.01850743509447 98-99 21.535098958618473 26.65411600974272 27.585695352572877 24.225089679065935 100 21.61448666124403 26.469887067290372 27.495688799722767 24.419937471742827 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2110.0 1 1499.5 2 1254.5 3 1661.0 4 1431.0 5 1311.5 6 2088.5 7 4124.5 8 6652.5 9 8048.5 10 7533.0 11 6691.5 12 7079.0 13 8088.0 14 9299.0 15 10590.5 16 11915.0 17 13290.0 18 14770.0 19 16239.5 20 17701.0 21 19718.5 22 22332.5 23 25778.5 24 30161.0 25 35839.5 26 43689.0 27 53361.5 28 64828.0 29 82128.5 30 106660.0 31 131718.0 32 163326.0 33 201087.5 34 236548.0 35 269194.5 36 306505.0 37 349634.5 38 369877.5 39 370588.0 40 364931.0 41 357344.5 42 359866.5 43 374251.0 44 393125.5 45 422719.0 46 449134.0 47 464313.5 48 480124.5 49 484616.0 50 474817.0 51 463147.5 52 450377.5 53 431762.0 54 411936.5 55 377669.0 56 337103.0 57 291190.0 58 248521.0 59 220664.5 60 183964.0 61 144619.0 62 115370.5 63 89920.0 64 66224.5 65 46791.0 66 33643.5 67 25355.0 68 18988.5 69 13910.0 70 10587.5 71 8255.0 72 6548.5 73 5231.5 74 3984.5 75 2769.5 76 1836.0 77 1077.0 78 648.0 79 406.0 80 245.5 81 121.5 82 81.0 83 60.0 84 37.5 85 26.0 86 18.5 87 13.0 88 8.5 89 5.5 90 2.5 91 2.0 92 2.5 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029219600244002077 2 0.001901715407261349 3 1.1992799865612112E-4 4 0.02351445402221803 5 5.139771370976619E-4 6 7.709657056464929E-5 7 1.713257123658873E-5 8 0.0037605993864312263 9 0.01004825303025929 10-11 0.0010279542741953238 12-13 9.85122846103852E-5 14-15 1.4134371270185702E-4 16-17 3.726334243958049E-4 18-19 1.2849428427441547E-5 20-21 5.5680856518913373E-5 22-23 3.3408513911348025E-4 24-25 2.5698856854883095E-5 26-27 7.709657056464929E-5 28-29 8.69477990256878E-4 30-31 0.002509921686160249 32-33 0.009667053320245192 34-35 0.018314718651913354 36-37 0.02114587604875964 38-39 0.02019073520231982 40-41 0.004475884235558806 42-43 0.004942746801755849 44-45 0.008750460759087694 46-47 0.011654431583689484 48-49 1.1977766034352004 50-51 0.017762193229533367 52-53 0.018293302937867618 54-55 0.009165925611574971 56-57 0.0075982953434271016 58-59 0.007812452483884461 60-61 0.0049170479449009655 62-63 0.007367005631733154 64-65 0.019012870929804346 66-67 0.02871847253533186 68-69 0.02893262967578922 70-71 0.026045791422424018 72-73 0.02267924117443433 74-75 0.009110244755056058 76-77 0.014451323838062595 78-79 0.007851000769166784 80-81 0.0026426991132438116 82-83 0.013680358132416102 84-85 0.023450206880080825 86-87 0.02294051288579231 88-89 0.03250048763580882 90-91 0.020344928343449118 92-93 0.013007904711379994 94-95 0.006321918786301241 96-97 0.003567857960019603 98-99 0.015179458115617616 100 0.03483908360960318 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1673671E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.92695127518709 #Duplication Level Percentage of deduplicated Percentage of total 1 69.92408882785772 35.610206646981645 2 15.901739468664658 16.196542222228093 3 5.98732077140264 9.147479795824136 4 2.9199183261121413 5.948101532857556 5 1.6294471956220211 4.1491388968466465 6 0.9494306663930104 2.901096557193916 7 0.6261254794374138 2.232066324842364 8 0.41037171815205326 1.6719184396035542 9 0.2878557269082908 1.319365312068788 >10 1.2527535563114043 10.837806042507387 >50 0.06551446259487004 2.2714056581628017 >100 0.040617307096246634 4.069343425251977 >500 0.0032471832518585477 1.137399568001889 >1k 0.0014610819089639915 1.1911414352371505 >5k 2.7057071673489994E-5 0.10614226917403334 >10k+ 8.117121502046999E-5 1.2108458732180976 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37773 0.32357430665983306 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30833 0.26412428446887015 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19268 0.16505519129329582 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.426514247317746E-5 2 0.0 0.0 0.0 0.0 3.426514247317746E-5 3 0.0 0.0 0.0 0.0 3.426514247317746E-5 4 0.0 0.0 0.0 0.0 3.426514247317746E-5 5 0.0 0.0 0.0 0.0 3.426514247317746E-5 6 0.0 0.0 0.0 0.0 5.139771370976619E-5 7 0.0 0.0 0.0 0.0 5.139771370976619E-5 8 0.0 0.0 0.0 0.0 5.139771370976619E-5 9 0.0 0.0 0.0 0.0 5.139771370976619E-5 10-11 0.0 0.0 0.0 8.566285618294365E-6 5.5680856518913373E-5 12-13 0.0 0.0 0.0 8.566285618294365E-6 8.566285618294366E-5 14-15 0.0 0.0 0.0 8.566285618294365E-6 1.2421114146526829E-4 16-17 0.0 0.0 0.0 1.713257123658873E-5 1.3706056989270984E-4 18-19 0.0 0.0 0.0 3.426514247317746E-5 1.5419314112929857E-4 20-21 0.0 0.0 0.0 9.422914180123802E-5 1.5419314112929857E-4 22-23 0.0 0.0 0.0 2.912537110220084E-4 1.6704256955674013E-4 24-25 0.0 0.0 0.0 9.551408464398217E-4 1.713257123658873E-4 26-27 0.0 0.0 0.0 0.0019402636925436737 1.8417514079332884E-4 28-29 0.0 0.0 0.0 0.005469573367280952 1.8845828360247602E-4 30-31 0.0 0.0 0.0 0.018922924930812254 1.8845828360247602E-4 32-33 0.0 0.0 0.0 0.04970587230015305 1.8845828360247602E-4 34-35 0.0 0.0 0.0 0.09742436633686181 2.1415714045735913E-4 36-37 0.0 0.0 0.0 0.1751548420372649 2.3128971169394786E-4 38-39 0.0 0.0 0.0 0.28352263825149776 2.3985599731224223E-4 40-41 0.0 0.0 0.0 0.4004395875127884 2.3985599731224223E-4 42-43 0.0 0.0 0.0 0.515433405652772 2.4413914012138941E-4 44-45 0.0 0.0 0.0 0.6544942032373535 2.5270542573968373E-4 46-47 0.0 0.0 0.0 0.8217337973633144 2.6983799697627246E-4 48-49 4.2831428091471824E-6 0.0 0.0 1.002028410771556 2.912537110220084E-4 50-51 8.566285618294365E-6 0.0 0.0 1.1921528369267902 2.912537110220084E-4 52-53 8.566285618294365E-6 0.0 0.0 1.3765507011461948 3.212357106860387E-4 54-55 8.566285618294365E-6 0.0 0.0 1.5796273511562902 3.383682819226274E-4 56-57 8.566285618294365E-6 0.0 0.0 1.8154143627998425 3.4693456754092183E-4 58-59 8.566285618294365E-6 0.0 0.0 2.0799069975502995 3.51217710350069E-4 60-61 8.566285618294365E-6 0.0 0.0 2.354572096472481 3.51217710350069E-4 62-63 8.566285618294365E-6 4.2831428091471824E-6 0.0 2.625660771149024 3.683502815866577E-4 64-65 8.566285618294365E-6 8.566285618294365E-6 0.0 2.9031184791827696 3.726334243958049E-4 66-67 8.566285618294365E-6 8.566285618294365E-6 0.0 3.214691419691372 3.7691656720495204E-4 68-69 8.566285618294365E-6 1.2849428427441547E-5 0.0 3.5574285072793295 3.7691656720495204E-4 70-71 8.566285618294365E-6 2.5698856854883095E-5 0.0 3.9271194125652507 3.7691656720495204E-4 72-73 8.566285618294365E-6 2.5698856854883095E-5 0.0 4.292634253612253 4.325974237238654E-4 74-75 8.566285618294365E-6 2.5698856854883095E-5 0.0 4.649223025044993 4.5829628057874857E-4 76-77 8.566285618294365E-6 2.5698856854883095E-5 0.0 5.036342038421333 4.7971199462448446E-4 78-79 8.566285618294365E-6 2.5698856854883095E-5 0.0 5.458595672261108 4.8827828024277883E-4 80-81 8.566285618294365E-6 2.5698856854883095E-5 0.0 5.907901636083457 5.011277086702204E-4 82-83 8.566285618294365E-6 2.5698856854883095E-5 0.0 6.36492154010508 5.225434227159563E-4 84-85 8.566285618294365E-6 2.5698856854883095E-5 0.0 6.806312256016124 5.268265655251035E-4 86-87 1.713257123658873E-5 2.5698856854883095E-5 0.0 7.264064577458111 5.525254223799866E-4 88 1.713257123658873E-5 2.5698856854883095E-5 0.0 7.626255699685215 5.653748508074281E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20510 0.0 50.838123 1 GTATCAA 37205 0.0 41.155407 1 CAACGCA 46495 0.0 32.146065 5 ATCAACG 46625 0.0 32.041668 3 TATCAAC 48665 0.0 31.245392 2 TCAACGC 49310 0.0 30.40162 4 AACGCAG 50150 0.0 30.046432 6 GTACTAG 5065 0.0 28.350002 1 GTGGTAT 9670 0.0 26.835506 1 GTACATG 52895 0.0 26.27735 1 ACGCAGA 56695 0.0 26.263372 7 TACATGG 53810 0.0 25.642225 2 CGCAGAG 58205 0.0 25.533667 8 ACATGGG 53505 0.0 24.95752 3 TGGTATC 10185 0.0 24.46004 2 GCAGAGT 63350 0.0 23.111 9 CATGGGG 34385 0.0 22.460617 4 CTGTGCG 6885 0.0 22.143742 9 CTAGTAC 7050 0.0 21.496824 3 TAGTACT 7350 0.0 21.385448 4 >>END_MODULE