FastQCFastQC Report
Mon 6 Jun 2016
ERR1211360_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211360_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11604764
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376940.3248148777519302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302660.26080668249694694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180480.1555223354822209No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187650.053.9662741
GTATCAA341300.041.7436071
CAACGCA418400.033.3094985
ATCAACG421850.032.9928133
TATCAAC438800.032.1239052
TCAACGC448050.031.2102264
AACGCAG450500.031.197896
GTACTAG48600.028.8407761
GTACATG496650.028.0897351
ACGCAGA507750.027.3666617
TACATGG507900.027.1144682
CGCAGAG523000.026.5045348
ACATGGG520450.025.6588633
GTGGTAT80800.025.6134621
GCAGAGT564150.023.7480899
TAGTACT68500.023.3912964
CATGGGG355500.022.9853154
GCATCGT52700.022.3801488
CTGTGCG72200.022.1293059
GAGTACT308700.021.89627812-13
Produced by FastQC (version 0.11.5)