FastQCFastQC Report
Mon 6 Jun 2016
ERR1211360_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211360_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11604764
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338170.29140618456351197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284810.24542506853219934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174870.1506881139504431No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA188550.052.618321
GTATCAA337350.041.1366731
CAACGCA418850.032.301115
ATCAACG423200.032.0310063
TATCAAC444000.030.9824662
AACGCAG450500.030.437776
TCAACGC447700.030.3095764
GTACTAG48100.028.2951151
GTACATG500850.026.555271
ACGCAGA515750.026.2140167
GTGGTAT77150.026.0355281
TACATGG511750.025.7072242
CGCAGAG531650.025.4388628
TAGTACT66100.024.77384
ACATGGG514550.024.575363
CTAGTAC69850.023.040743
GCAGAGT581550.022.9406859
TGGTATC88300.022.4757732
TCTAACG23300.021.9485362
CATGGGG353200.021.9461354
Produced by FastQC (version 0.11.5)