##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211360_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11604764 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.095258292197926 33.0 31.0 34.0 30.0 34.0 2 32.22958476363673 33.0 31.0 34.0 30.0 34.0 3 32.29434730426228 34.0 31.0 34.0 30.0 34.0 4 35.935745268064046 37.0 35.0 37.0 35.0 37.0 5 35.839710053560765 37.0 35.0 37.0 35.0 37.0 6 35.85365475764953 37.0 35.0 37.0 35.0 37.0 7 35.76766265992139 37.0 35.0 37.0 33.0 37.0 8 35.75033606887654 37.0 35.0 37.0 33.0 37.0 9 37.36025041095191 39.0 37.0 39.0 34.0 39.0 10-11 37.31223319147205 39.0 37.0 39.0 34.0 39.0 12-13 37.235876102262836 39.0 37.0 39.0 33.0 39.0 14-15 38.46364609396623 40.0 38.0 41.0 34.0 41.0 16-17 38.445607984789696 40.0 38.0 41.0 34.0 41.0 18-19 38.450850960864 40.0 38.0 41.0 34.0 41.0 20-21 38.41752111460431 40.0 38.0 41.0 34.0 41.0 22-23 38.307855118811545 40.0 38.0 41.0 34.0 41.0 24-25 38.21342790771101 40.0 38.0 41.0 33.0 41.0 26-27 38.11233933753414 40.0 37.5 41.0 33.0 41.0 28-29 38.01012123124606 40.0 37.0 41.0 33.0 41.0 30-31 38.02139078399182 40.0 37.0 41.0 33.0 41.0 32-33 37.9625687347024 40.0 37.0 41.0 33.0 41.0 34-35 37.83617478132257 40.0 37.0 41.0 33.0 41.0 36-37 37.690688151865906 40.0 37.0 41.0 33.0 41.0 38-39 37.57510027778247 40.0 37.0 41.0 32.0 41.0 40-41 37.46045063906513 40.0 36.0 41.0 32.0 41.0 42-43 37.34954377357437 40.0 36.0 41.0 31.5 41.0 44-45 37.18334767514445 39.0 36.0 41.0 31.0 41.0 46-47 36.93444248413841 39.0 35.5 41.0 31.0 41.0 48-49 35.37981806437425 39.0 35.0 40.5 24.0 41.0 50-51 36.0964038562094 39.0 35.0 40.0 28.5 41.0 52-53 36.182658475433016 39.0 35.0 40.0 29.0 41.0 54-55 36.00873697216075 38.0 35.0 40.0 29.0 41.0 56-57 35.785246343656794 38.0 34.5 40.0 28.0 41.0 58-59 35.53355376292012 38.0 34.0 40.0 28.0 41.0 60-61 35.27041385761916 37.0 34.0 40.0 28.0 41.0 62-63 34.94392389194645 37.0 34.0 40.0 27.0 41.0 64-65 34.60193563608877 36.0 33.5 39.0 26.5 41.0 66-67 34.26190528303721 36.0 33.0 39.0 26.0 40.0 68-69 33.8986394294619 35.0 33.0 38.5 26.0 40.0 70-71 33.54545017029213 35.0 32.5 38.0 26.0 40.0 72-73 33.1730012777511 35.0 32.0 37.0 25.5 39.5 74-75 32.815127003013586 35.0 32.0 37.0 25.0 39.0 76-77 31.89461608180916 34.0 30.5 35.5 24.5 38.0 78-79 32.34021988728078 34.5 31.5 36.0 25.0 38.0 80-81 32.225164768538164 34.5 31.5 35.0 25.0 37.0 82-83 31.966086901896496 34.0 31.0 35.0 24.5 37.0 84-85 31.696159956376533 34.0 31.0 35.0 24.0 36.0 86-87 31.447608671748945 34.0 31.0 35.0 24.0 36.0 88-89 31.22824458127714 34.0 31.0 35.0 23.0 35.5 90-91 31.013886021292635 34.0 30.5 35.0 22.5 35.0 92-93 30.82194911503586 34.0 30.0 35.0 20.5 35.0 94-95 30.627664853847953 34.0 30.0 35.0 20.0 35.0 96-97 30.44327170289719 34.0 29.5 35.0 20.0 35.0 98-99 30.230574055620608 34.0 29.0 35.0 19.0 35.0 100 27.752342744755516 31.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.26429504222780764 1101 2 -0.27479239946285006 1101 3 -0.2557889297572089 1101 4 -0.16169274894393482 1101 5 -0.13562689892051338 1101 6 -0.17759993308748534 1101 7 -0.19521177331558448 1101 8 -0.16568317105383557 1101 9 -0.2909909351401865 1101 10-11 -0.2528836852251146 1101 12-13 -0.36925570558975096 1101 14-15 -0.4614213008932495 1101 16-17 -0.4512534544710505 1101 18-19 -0.5193058771237915 1101 20-21 -0.5337924054585983 1101 22-23 -0.6005694911804866 1101 24-25 -0.538810141039221 1101 26-27 -0.5935767799531604 1101 28-29 -0.5992013902827793 1101 30-31 -0.5871410495271476 1101 32-33 -0.5219976766419308 1101 34-35 -0.5143395590394348 1101 36-37 -0.5854305581062249 1101 38-39 -0.542422597456877 1101 40-41 -0.5385232569088672 1101 42-43 -0.5349463662611527 1101 44-45 -0.5034419909076107 1101 46-47 -0.5340465279185409 1101 48-49 0.6861927064132445 1101 50-51 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1105 68-69 -0.8320162545684227 1105 70-71 -0.8910007686790635 1105 72-73 -0.810267253439946 1105 74-75 -0.8841549353048919 1105 76-77 -0.8767729569117577 1105 78-79 -0.7650767144912187 1105 80-81 -0.715521331642119 1105 82-83 -0.6526314983834105 1105 84-85 -0.6788562843397408 1105 86-87 -0.6520981081765633 1105 88-89 -0.6797977798152957 1105 90-91 -0.6893042456864471 1105 92-93 -0.7003554046705354 1105 94-95 -0.7693114294461125 1105 96-97 -0.7909123986637816 1105 98-99 -0.7983514220994117 1105 100 -0.7815131014130792 1106 1 -0.3155372708902746 1106 2 -0.3108107748949607 1106 3 -0.3478693677580118 1106 4 -0.23869822692617504 1106 5 -0.25997634916558354 1106 6 -0.333361109649573 1106 7 -0.31380822409487763 1106 8 -0.2944687756561635 1106 9 -0.44309357761962787 1106 10-11 -0.4307850576964114 1106 12-13 -0.42763341497384033 1106 14-15 -0.5897025155872839 1106 16-17 -0.5497304623232537 1106 18-19 -0.5789203767467157 1106 20-21 -0.6013117617530455 1106 22-23 -0.6406620933462435 1106 24-25 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2311 3 0.3853709033173942 2311 4 0.26539270085272193 2311 5 0.3330139600255251 2311 6 0.3754858005806838 2311 7 0.3797733066657756 2311 8 0.17125125127321894 2311 9 0.4305437647998005 2311 10-11 0.3758356602833999 2311 12-13 0.4770274495415521 2311 14-15 0.5027768291310863 2311 16-17 0.46973824340472703 2311 18-19 0.6290790587983608 2311 20-21 0.7261487135539753 2311 22-23 0.7123642929944651 2311 24-25 0.7220297414558488 2311 26-27 0.711538649022522 2311 28-29 0.7179179341030064 2311 30-31 0.6101122840073501 2311 32-33 0.6385032643809723 2311 34-35 0.7701245664183674 2311 36-37 0.7970820345012655 2311 38-39 0.8365485862314657 2311 40-41 0.750499437165935 2311 42-43 0.7961320884495962 2311 44-45 0.826847379221725 2311 46-47 0.8833725938455714 2311 48-49 -6.018137088584581 2311 50-51 -1.023134078427887 2311 52-53 0.41836613878452766 2311 54-55 0.8754011421324392 2311 56-57 0.9340573820413667 2311 58-59 0.9299338099319243 2311 60-61 0.794027064963089 2311 62-63 0.6933370068359039 2311 64-65 0.8336675689410669 2311 66-67 0.9120425206019078 2311 68-69 0.9179493534977539 2311 70-71 0.9467611552560484 2311 72-73 0.42539466546219273 2311 74-75 0.7264080019340184 2311 76-77 0.802226783051669 2311 78-79 0.7355087872116073 2311 80-81 0.8259475814050106 2311 82-83 0.8505814212401255 2311 84-85 0.9053332823218945 2311 86-87 0.8780317423443513 2311 88-89 0.9349402123668291 2311 90-91 1.0089027602021226 2311 92-93 0.9698899104770149 2311 94-95 1.0108832205111042 2311 96-97 1.0307121588546302 2311 98-99 1.1065978707688764 2311 100 1.3556352200927826 2312 1 0.06705786783236789 2312 2 0.24286639546551925 2312 3 0.22226375001499576 2312 4 0.014503723435730365 2312 5 0.20999326994201084 2312 6 0.13225876817708127 2312 7 0.278730262541238 2312 8 0.31105204913853157 2312 9 0.24378109149213145 2312 10-11 0.26497107792052077 2312 12-13 0.3270715629557941 2312 14-15 0.4113846922805422 2312 16-17 0.4727640678864091 2312 18-19 0.36212574763876404 2312 20-21 0.3789298285167533 2312 22-23 0.6591588341013974 2312 24-25 0.7949322917140904 2312 26-27 0.696062888109573 2312 28-29 0.806890716114836 2312 30-31 0.8352154065001187 2312 32-33 0.8352865251073496 2312 34-35 0.39274328793352 2312 36-37 0.7076167511904785 2312 38-39 0.8276698896617631 2312 40-41 0.6560266266489734 2312 42-43 0.656694394852309 2312 44-45 0.8235497305930082 2312 46-47 0.8821827135809954 2312 48-49 -4.607722892941183 2312 50-51 -0.6732131222038689 2312 52-53 0.6046665134762463 2312 54-55 0.8746780564135932 2312 56-57 0.9765620355604483 2312 58-59 1.0023771380416306 2312 60-61 0.986934335947609 2312 62-63 0.7582425668178701 2312 64-65 0.9748521340432887 2312 66-67 0.9774122795412694 2312 68-69 0.9065034810162231 2312 70-71 0.983773433779362 2312 72-73 1.0365125088805485 2312 74-75 0.7067658176908225 2312 76-77 0.9080072799399765 2312 78-79 0.5968992137548312 2312 80-81 0.7993631195701241 2312 82-83 0.7570679876800064 2312 84-85 0.8998631668135779 2312 86-87 0.9687806483733858 2312 88-89 0.9525729535352276 2312 90-91 0.7338350083941343 2312 92-93 0.9330477646577897 2312 94-95 1.0683452183169067 2312 96-97 1.127695714412038 2312 98-99 1.1404084389385325 2312 100 0.681386747202545 2313 1 0.09837303666365216 2313 2 0.22526071157257377 2313 3 0.3141785556766976 2313 4 0.08363102984456816 2313 5 0.15894112110429148 2313 6 0.2694583469740408 2313 7 0.18883652495139813 2313 8 0.2546038010240821 2313 9 0.45760751314674764 2313 10-11 0.2469348433201759 2313 12-13 0.03601447998484275 2313 14-15 0.13154183482595982 2313 16-17 0.09467023750852377 2313 18-19 0.4108265942730398 2313 20-21 0.365428127269098 2313 22-23 0.47494526234692813 2313 24-25 0.2681819306249338 2313 26-27 0.42382723453828675 2313 28-29 0.38274821845401874 2313 30-31 0.5791194137388445 2313 32-33 0.5176812790143686 2313 34-35 0.41231803518621035 2313 36-37 -0.2699607870427201 2313 38-39 0.20981243769860924 2313 40-41 0.4073778753061248 2313 42-43 0.5355584947671375 2313 44-45 0.5008161602817722 2313 46-47 0.4254128114011806 2313 48-49 -5.150621691161724 2313 50-51 -1.2584539752941382 2313 52-53 0.10898142395910071 2313 54-55 0.3140771450859532 2313 56-57 0.3528135177949068 2313 58-59 0.4205088716763825 2313 60-61 0.7192136511806382 2313 62-63 0.7938445868709891 2313 64-65 0.8185845875698021 2313 66-67 0.8657968533335492 2313 68-69 0.8817206562126998 2313 70-71 0.8489961341970655 2313 72-73 0.6288473789536511 2313 74-75 0.7212072327350612 2313 76-77 0.734966319480737 2313 78-79 0.7109261483693174 2313 80-81 -0.19916858415487937 2313 82-83 0.26160319747426186 2313 84-85 0.6676484386865944 2313 86-87 0.7429045377942209 2313 88-89 0.766722814131942 2313 90-91 0.8069444479661811 2313 92-93 0.8613229835696679 2313 94-95 0.7933247273629291 2313 96-97 0.8388915970393356 2313 98-99 1.0041285187519016 2313 100 1.0864220900264456 2314 1 0.0697574689120728 2314 2 0.16012320902024868 2314 3 0.19290278786895954 2314 4 0.042979266852022135 2314 5 0.18032657559292886 2314 6 0.25257938265873037 2314 7 0.07777283945298308 2314 8 0.2025063207986122 2314 9 0.3675534996215504 2314 10-11 0.2839832276554688 2314 12-13 0.1796284284889893 2314 14-15 0.23271361099691745 2314 16-17 0.4348095244586503 2314 18-19 0.5111683325435123 2314 20-21 0.5726688058146152 2314 22-23 0.29585470802293656 2314 24-25 0.41918116250032966 2314 26-27 0.4286625893792362 2314 28-29 0.5794382063172208 2314 30-31 0.5340904393099493 2314 32-33 0.4681736066548865 2314 34-35 0.37921197742188184 2314 36-37 0.4377963579835864 2314 38-39 0.48378764299926047 2314 40-41 0.23184463899274732 2314 42-43 0.2500748267786719 2314 44-45 0.48262144506829685 2314 46-47 0.5959719598758042 2314 48-49 -6.519448305846083 2314 50-51 -1.6986067536998846 2314 52-53 -0.9297565261234766 2314 54-55 -0.45346244161850535 2314 56-57 -0.12109155342217548 2314 58-59 0.3499746532832262 2314 60-61 0.5944890152243687 2314 62-63 0.514596033118238 2314 64-65 0.6721638618974879 2314 66-67 0.697471799292444 2314 68-69 0.7505715591909023 2314 70-71 0.5347443444821138 2314 72-73 0.6953134319759897 2314 74-75 0.7061469626154988 2314 76-77 0.6826695149379525 2314 78-79 0.5364003516324303 2314 80-81 0.5690242037475528 2314 82-83 0.5794632780544013 2314 84-85 0.5995463003632331 2314 86-87 0.624786679570537 2314 88-89 0.5708065560723767 2314 90-91 0.7560242695193864 2314 92-93 0.8390246042815726 2314 94-95 0.8764516788488557 2314 96-97 0.8935361280483569 2314 98-99 0.9578969272789628 2314 100 -0.4063707157091123 2315 1 0.15968639947087127 2315 2 0.23460387478913702 2315 3 0.32658384385226924 2315 4 0.15214704068783647 2315 5 0.254033298939909 2315 6 0.30204042123907726 2315 7 0.3696854475802738 2315 8 0.30023372726336106 2315 9 0.4566633838292091 2315 10-11 0.253408265317681 2315 12-13 0.42608186214992116 2315 14-15 0.25134701635840173 2315 16-17 0.395000165114169 2315 18-19 0.46460811674781866 2315 20-21 0.3797991726805563 2315 22-23 0.4699823990132188 2315 24-25 0.52006271694313 2315 26-27 0.5564403387428953 2315 28-29 0.6141295864983292 2315 30-31 0.5920746783002286 2315 32-33 0.7263660903555262 2315 34-35 0.7148536410118354 2315 36-37 0.6204288114078906 2315 38-39 0.6978808722320835 2315 40-41 0.7125191293227076 2315 42-43 0.7962460511741156 2315 44-45 0.7639221161048297 2315 46-47 0.6465244322995929 2315 48-49 -4.697111685233114 2315 50-51 -0.8170522890913858 2315 52-53 0.3087128386683773 2315 54-55 0.2466809198464759 2315 56-57 0.6131321968325736 2315 58-59 0.7563221676292358 2315 60-61 0.5831596601172038 2315 62-63 0.31068749411564056 2315 64-65 0.548432636189915 2315 66-67 -0.1643403259828915 2315 68-69 0.5625374631285993 2315 70-71 0.5609622519895723 2315 72-73 0.6921218437017487 2315 74-75 0.7908690284086077 2315 76-77 0.7744199446684696 2315 78-79 0.73010435565741 2315 80-81 0.6434024478280307 2315 82-83 0.5745126638034428 2315 84-85 0.6003171075386184 2315 86-87 0.6348606083565862 2315 88-89 0.6238565904194111 2315 90-91 0.6960711638652235 2315 92-93 0.7664035895452379 2315 94-95 0.3846298740042222 2315 96-97 0.4974386288387649 2315 98-99 0.4762307775553829 2315 100 0.45914374040555117 2316 1 0.20870761685717554 2316 2 0.22390813984949887 2316 3 0.15107142591962486 2316 4 -0.06621913922127476 2316 5 0.14016353339939513 2316 6 0.11665160909008421 2316 7 0.22203080771356554 2316 8 0.28324990589984367 2316 9 0.4157502086652798 2316 10-11 0.3931071592141535 2316 12-13 0.31756375294428096 2316 14-15 0.46494038177517183 2316 16-17 0.4369423422826557 2316 18-19 0.1617027591357143 2316 20-21 0.4280463491215798 2316 22-23 0.5668618779353878 2316 24-25 0.5907253456936061 2316 26-27 0.6003550850443631 2316 28-29 0.6541757027364454 2316 30-31 0.5734581678086101 2316 32-33 0.5288932332473095 2316 34-35 0.52224423019765 2316 36-37 0.4572520083251703 2316 38-39 0.5265705098613793 2316 40-41 0.13734398917016932 2316 42-43 0.1038022057011645 2316 44-45 0.3944932955186573 2316 46-47 0.36991271789845825 2316 48-49 -5.236340927540937 2316 50-51 -1.0825061291281415 2316 52-53 0.2291691048685962 2316 54-55 0.5388109333586968 2316 56-57 0.4097831862326089 2316 58-59 0.32222520260795307 2316 60-61 0.4822426376160962 2316 62-63 0.377194124855194 2316 64-65 0.34667681378456905 2316 66-67 0.43725727341801246 2316 68-69 0.1623061209404142 2316 70-71 -0.26743327834920194 2316 72-73 -0.11697197199340792 2316 74-75 0.08850243558307369 2316 76-77 0.15138740823861951 2316 78-79 0.18463395510955394 2316 80-81 0.15115054249647386 2316 82-83 0.12005729386059372 2316 84-85 0.21179782437597794 2316 86-87 0.2604346133501032 2316 88-89 0.17407618551366966 2316 90-91 0.23033216685830737 2316 92-93 0.3283329112289728 2316 94-95 0.5112680610173541 2316 96-97 0.5745055949916598 2316 98-99 0.5938020922719929 2316 100 -0.09602501532128116 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 9.0 11 23.0 12 61.0 13 229.0 14 897.0 15 2691.0 16 6257.0 17 12909.0 18 22380.0 19 33838.0 20 49542.0 21 69987.0 22 87776.0 23 93960.0 24 98183.0 25 108496.0 26 128588.0 27 157524.0 28 192955.0 29 238098.0 30 292485.0 31 362375.0 32 455848.0 33 595302.0 34 825600.0 35 1158290.0 36 1793634.0 37 2582400.0 38 1984193.0 39 250207.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.72377140337234 19.518415394929857 13.816687851026686 23.941125350671122 2 15.642036141491658 19.941097277560875 40.12666748071469 24.29019910023278 3 19.117839424139653 24.996790109711895 29.458494524401114 26.42687594174734 4 12.89521177353076 16.227416300781513 37.39711567492764 33.48025625076009 5 13.596734286160657 37.764478175248264 34.26008499493868 14.378702543652391 6 32.151605401853274 36.40050055330753 17.174507215057826 14.273386829781371 7 28.48174124396013 32.00496761630851 21.3182244770056 18.195066662725758 8 26.70125724784272 35.51476325832244 19.252123024926505 18.531856468908334 9 26.676246399413152 15.719371053832651 20.038248263651894 37.5661342831023 10-11 25.78592410922138 25.992923009920766 27.116993867372656 21.104159013485198 12-13 25.95877836105882 23.354285521507588 27.843445546392214 22.84349057104138 14-15 23.281504414937658 24.880000651457348 25.33215599939008 26.506338934214913 16-17 22.629379774430607 27.87689476218796 26.23516212775981 23.258563335621627 18-19 22.403766006038385 27.06303042346154 28.429508371607138 22.103695198892936 20-21 23.26971844248453 26.19442561954041 28.341761562063606 22.19409437591145 22-23 23.273677652857977 25.9182405250202 28.11242256339428 22.69565925872755 24-25 22.9435570355231 26.04789121428545 28.401834971325123 22.606716778866325 26-27 22.791219874199832 26.317554857259363 28.08432647516699 22.80689879337382 28-29 22.504186361541787 26.5186641817427 28.25957875928588 22.71757069742963 30-31 23.094095822828407 26.21624805387644 28.246283180830435 22.443372942464716 32-33 22.99476796509943 26.42091876328832 27.906926639909123 22.67738663170313 34-35 23.06786285350331 26.41854774781996 28.181266390308636 22.332323008368093 36-37 22.975061664083 26.246567729477526 28.049482614684678 22.728887991754792 38-39 22.990471445169536 26.3267132180757 28.196276619882116 22.48653871687265 40-41 22.92034923121151 25.956534650462004 28.419093137744923 22.704022980581566 42-43 22.779356713454487 26.114781250861757 28.08947394727408 23.016388088409673 44-45 22.686730827766436 26.482931145802702 28.05165383341256 22.7786841930183 46-47 22.71637797998799 26.297786554721135 27.7210096039676 23.26482586132327 48-49 23.11197493929206 26.44774669270652 27.55737246318004 22.882905904821378 50-51 22.935231181770487 26.435115367336454 27.49601867682817 23.133634774064895 52-53 22.731651072117028 26.668071816576997 27.390796064109313 23.20948104719666 54-55 22.626190235194702 26.56870690119132 27.46324936012544 23.341853503488537 56-57 22.954235231977968 26.465181049133825 27.14345657501798 23.43712714387023 58-59 22.619552736987533 26.52562058424866 27.563246608763727 23.29158007000008 60-61 22.819919865186687 26.76318779544811 27.292319717136778 23.124572622228428 62-63 22.826515130949904 26.53851109091669 27.223080413293328 23.41189336484008 64-65 22.476172565416363 26.419055673150076 27.347076536263305 23.75769522517026 66-67 22.696721395198953 26.375441233949637 27.187760353512097 23.74007701733931 68-69 22.445679403157083 26.495129565433068 27.290379957035277 23.768811074374575 70-71 22.502929526306694 26.22938611178271 27.33006484822705 23.93761951368355 72-73 22.66291842797211 26.47607369080599 27.121139530988064 23.739868350233838 74-75 22.622270636044732 26.609489329448927 27.055012360318916 23.713227674187422 76-77 22.478001057996952 26.442443018038865 26.986463396115056 24.093092527849123 78-79 22.407976921991068 26.357614341678836 27.098528461516054 24.13588027481404 80-81 22.296670789229495 26.684123789945087 26.969954238173827 24.04925118265159 82-83 22.662781263536267 26.32530259622311 27.282892671828858 23.729023468411768 84-85 22.648584919991865 26.41490008189553 26.876433511134934 24.060081486977673 86-87 22.093085494245667 26.660983185376647 27.179430624465827 24.06650069591186 88-89 22.30770722683701 26.179741479071406 27.176268070109504 24.33628322398208 90-91 22.443525828772014 26.106672335627536 27.17222265484488 24.277579180755573 92-93 22.464221170797302 26.228210151246696 27.03262648638472 24.274942191571277 94-95 22.026392254893747 26.05885613741329 27.26019042185769 24.654561185835284 96-97 22.033468380671138 25.942480416355075 27.276366589494298 24.74768461347949 98-99 21.79003370539536 26.096211781077862 27.166780494609235 24.946974018917544 100 21.90898385553919 25.943370732123185 27.02397528588709 25.12367012645053 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1807.0 1 1327.0 2 1167.0 3 1581.0 4 1366.5 5 1247.0 6 2065.5 7 4039.0 8 6460.0 9 7819.0 10 7317.0 11 6468.5 12 6810.0 13 7688.5 14 8813.0 15 10081.5 16 11116.0 17 12197.5 18 13482.0 19 14740.0 20 16134.0 21 17555.0 22 19443.5 23 22366.5 24 25985.0 25 30747.0 26 37790.5 27 46224.0 28 56358.0 29 71811.0 30 92397.5 31 114234.5 32 141418.5 33 173388.5 34 202907.0 35 230795.5 36 264518.5 37 302965.0 38 323931.0 39 328762.0 40 327645.5 41 326338.0 42 335060.5 43 353235.0 44 376646.5 45 410320.0 46 441866.0 47 461766.0 48 480410.0 49 490808.5 50 487846.0 51 485598.5 52 479330.0 53 464424.5 54 448222.5 55 414850.5 56 375774.5 57 330702.0 58 285805.0 59 253732.5 60 213047.5 61 170227.5 62 136821.0 63 107528.5 64 79360.5 65 56437.0 66 40839.5 67 29984.0 68 22743.5 69 17416.5 70 13555.5 71 10328.0 72 7948.0 73 6503.0 74 5069.5 75 3682.0 76 2499.0 77 1526.5 78 927.5 79 568.5 80 340.5 81 203.0 82 135.5 83 96.5 84 55.5 85 41.0 86 31.5 87 13.5 88 8.5 89 7.5 90 3.5 91 3.0 92 3.0 93 0.5 94 0.5 95 0.5 96 0.0 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029625764039665086 2 0.0017837501908698876 3 5.170290408318515E-5 4 0.021844476975145726 5 6.290519996787527E-4 6 5.170290408318515E-5 7 2.5851452041592574E-5 8 0.003869100655558355 9 0.009478865748583944 10-11 9.737380268999869E-4 12-13 8.186293146504315E-5 14-15 1.1633153418716657E-4 16-17 3.6622890392256144E-4 18-19 2.5851452041592574E-5 20-21 1.0771438350663573E-4 22-23 2.5420594507566033E-4 24-25 4.308575340265429E-5 26-27 8.617150680530857E-5 28-29 0.0010038980542818449 30-31 0.002455887943951294 32-33 0.009108328269321116 34-35 0.017764256127914362 36-37 0.020560521523746624 38-39 0.020013332455532915 40-41 0.004584324162042417 42-43 0.005204759011040638 44-45 0.009099711118640587 46-47 0.011913210815833911 48-49 1.1978399560732127 50-51 0.01853979968916214 52-53 0.019009434401251073 54-55 0.009672751638895888 56-57 0.007970864379491044 58-59 0.007932087201428656 60-61 0.004903158737222058 62-63 0.007746818461797241 64-65 0.019621252099568765 66-67 0.02887176335511864 68-69 0.029181980779617752 70-71 0.02635986393174389 72-73 0.023201678207329334 74-75 0.009470248597903413 76-77 0.014700859060985644 78-79 0.008530979173725549 80-81 0.0028608940259362446 82-83 0.014459578841930781 84-85 0.024020307521979766 86-87 0.023438649851043932 88-89 0.032478040914920805 90-91 0.020702704509975387 92-93 0.01351600084241265 94-95 0.006820474763640174 96-97 0.003774311998072516 98-99 0.014868893499255995 100 0.033977425133333176 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1604764E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.42018856678695 #Duplication Level Percentage of deduplicated Percentage of total 1 69.77322922422374 35.1797937439901 2 15.784614681390178 15.917264973795328 3 6.042186281935162 9.139445150724727 4 2.921151497388654 5.891400373219519 5 1.5698459838150645 3.9575965262384356 6 0.9664509765274639 2.9237184286242073 7 0.6413716868963912 2.2636656976299996 8 0.4552520697134208 1.836311616029657 9 0.3258929335892151 1.4788424847736443 >10 1.404801213765851 12.070063417798572 >50 0.0703672817262188 2.418700853893995 >100 0.04090665384182539 3.896525474583328 >500 0.002806887049223287 0.9930289399755593 >1k 0.0010157111760792264 0.8636558221789744 >5k 2.6729240384045783E-5 0.09472936489745862 >10k+ 8.018772115213735E-5 1.0752571316465696 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33817 0.29140618456351197 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28481 0.24542506853219934 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17487 0.1506881139504431 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 8.617150680530859E-6 1.7234301361061717E-5 2 0.0 0.0 0.0 8.617150680530859E-6 1.7234301361061717E-5 3 0.0 0.0 0.0 1.7234301361061717E-5 1.7234301361061717E-5 4 0.0 0.0 0.0 2.5851452041592574E-5 1.7234301361061717E-5 5 0.0 0.0 0.0 2.5851452041592574E-5 1.7234301361061717E-5 6 0.0 0.0 0.0 3.4468602722123434E-5 3.4468602722123434E-5 7 0.0 0.0 0.0 4.308575340265429E-5 3.4468602722123434E-5 8 0.0 0.0 0.0 4.308575340265429E-5 3.4468602722123434E-5 9 0.0 0.0 0.0 6.893720544424687E-5 6.032005476371601E-5 10-11 0.0 0.0 0.0 1.034058081663703E-4 7.324578078451229E-5 12-13 0.0 0.0 0.0 1.3787441088849374E-4 8.617150680530857E-5 14-15 0.0 4.308575340265429E-6 0.0 1.3787441088849374E-4 1.20640109527432E-4 16-17 0.0 8.617150680530859E-6 0.0 1.637258629300863E-4 1.2925726020796287E-4 18-19 0.0 8.617150680530859E-6 0.0 2.3266306837433316E-4 1.2925726020796287E-4 20-21 0.0 8.617150680530859E-6 0.0 3.3606887654070343E-4 1.378744108884937E-4 22-23 0.0 8.617150680530859E-6 0.0 6.161262736579564E-4 1.5510871224955544E-4 24-25 0.0 8.617150680530857E-5 0.0 0.0012020925199340547 1.637258629300863E-4 26-27 0.0 8.617150680530857E-5 0.0 0.002098276190709264 1.7234301361061715E-4 28-29 0.0 8.617150680530857E-5 0.0 0.005463273531456564 1.7234301361061715E-4 30-31 0.0 1.034058081663703E-4 0.0 0.018712142702772758 1.8957731497167888E-4 32-33 0.0 1.034058081663703E-4 0.0 0.04981574808414889 2.9298312313804915E-4 34-35 0.0 1.034058081663703E-4 0.0 0.09774864874460179 3.059088491588455E-4 36-37 0.0 1.2494868486769744E-4 0.0 0.17309270571982333 3.2314315051990716E-4 38-39 0.0 1.5080013690929E-4 0.0 0.2815998670890679 3.3606887654070343E-4 40-41 0.0 1.637258629300863E-4 0.0 0.39835795023492077 3.4037745188096884E-4 42-43 0.0 1.637258629300863E-4 0.0 0.512005241985102 3.662289039225615E-4 44-45 0.0 1.637258629300863E-4 0.0 0.6503665218870457 3.705374792628269E-4 46-47 0.0 1.637258629300863E-4 0.0 0.8108049418325094 3.834632052836232E-4 48-49 0.0 1.637258629300863E-4 0.0 0.9859528379896394 4.0069750664468485E-4 50-51 4.308575340265429E-6 1.7234301361061718E-4 0.0 1.175521535810638 4.2224038334601205E-4 52-53 8.617150680530859E-6 1.8096016429114802E-4 0.0 1.3582654502926557 4.351661093668083E-4 54-55 8.617150680530859E-6 1.8096016429114802E-4 0.0 1.5593552785735239 4.5240041072787006E-4 56-57 8.617150680530859E-6 2.068116163327406E-4 0.0 1.7910790775236791 4.5670898606813546E-4 58-59 8.617150680530859E-6 2.068116163327406E-4 0.0 2.053591955855371 4.6532613674866633E-4 60-61 8.617150680530859E-6 2.068116163327406E-4 0.0 2.321266507444701 4.6532613674866633E-4 62-63 8.617150680530859E-6 2.068116163327406E-4 0.0 2.5930600570593247 4.739432874291972E-4 64-65 8.617150680530859E-6 2.068116163327406E-4 0.0 2.8668872542345536 4.739432874291972E-4 66-67 8.617150680530859E-6 2.068116163327406E-4 0.0 3.1738947901051673 4.739432874291972E-4 68-69 8.617150680530859E-6 2.068116163327406E-4 0.0 3.513669041438499 4.782518627694626E-4 70-71 8.617150680530859E-6 2.068116163327406E-4 0.0 3.8754342613085457 4.868690134499935E-4 72-73 8.617150680530859E-6 2.240459176938023E-4 0.0 4.23311495175602 4.997947394707897E-4 74-75 8.617150680530859E-6 2.240459176938023E-4 0.0 4.580722193057954 5.041033148110551E-4 76-77 8.617150680530859E-6 2.2835449303406773E-4 0.0 4.959902674453354 5.213376161721169E-4 78-79 8.617150680530859E-6 2.3266306837433316E-4 0.0 5.377433784952455 5.342633421929132E-4 80-81 8.617150680530859E-6 2.3266306837433316E-4 0.0 5.816736126645919 5.42880492873444E-4 82-83 8.617150680530859E-6 2.3266306837433316E-4 0.0 6.262884794555063 5.644233695747713E-4 84-85 8.617150680530859E-6 2.3266306837433316E-4 0.0 6.696155130772155 5.773490955955675E-4 86-87 8.617150680530859E-6 2.3266306837433316E-4 0.0 7.146625299747587 5.773490955955675E-4 88 8.617150680530859E-6 2.3266306837433316E-4 0.0 7.4996527288275745 5.859662462760983E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 18855 0.0 52.61832 1 GTATCAA 33735 0.0 41.136673 1 CAACGCA 41885 0.0 32.30111 5 ATCAACG 42320 0.0 32.031006 3 TATCAAC 44400 0.0 30.982466 2 AACGCAG 45050 0.0 30.43777 6 TCAACGC 44770 0.0 30.309576 4 GTACTAG 4810 0.0 28.295115 1 GTACATG 50085 0.0 26.55527 1 ACGCAGA 51575 0.0 26.214016 7 GTGGTAT 7715 0.0 26.035528 1 TACATGG 51175 0.0 25.707224 2 CGCAGAG 53165 0.0 25.438862 8 TAGTACT 6610 0.0 24.7738 4 ACATGGG 51455 0.0 24.57536 3 CTAGTAC 6985 0.0 23.04074 3 GCAGAGT 58155 0.0 22.940685 9 TGGTATC 8830 0.0 22.475773 2 TCTAACG 2330 0.0 21.948536 2 CATGGGG 35320 0.0 21.946135 4 >>END_MODULE