FastQCFastQC Report
Mon 6 Jun 2016
ERR1211359_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211359_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6020811
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363020.6029420289060726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281390.4673622872400413No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA224750.37328858188705805No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC193250.32097004871935025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185060.30736723009574624No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT168780.28032768343002296No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC157240.2611608303266786No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC155910.25895182559293095No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA150180.24943483527385263No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC129080.21438972258056266No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT128260.2130277798123874No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC111070.18447680885515258No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG107180.17801588523539438No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC96730.16065941947023415No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC93610.1554773933279088No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA92800.15413205961788204No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT90960.15107599291856197No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT87040.14456524212435834No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG85850.14258876420468938No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT85140.14140952107614738No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC80550.1337859633859957No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG79590.13219149380374173No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC78190.12986622566295472No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT77610.12890290029034293No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT76750.1274745212895738No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC75660.12566413395138962No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT75130.12478385386952023No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA72000.11958521866904642No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA70640.11732638676085332No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG70440.11699420559788375No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA70110.11644610667898393No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT65760.10922116638439573No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC65380.10859002217475354No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA60760.10091663731015639No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141800.056.1273271
GTATCAA270150.043.0421261
TACCTGG13550.036.101582
GCATCGT53400.035.2871368
GTACTAG56800.035.19581
TAGGCAT65050.034.6020245
CAACGCA332350.033.834545
ATCAACG334100.033.6438143
TATTCTC33800.033.505315
TACCCCG3100.033.3483475
ACCTGGG14700.032.9261053
TATCAAC357450.032.2391052
CTGTGCG66500.031.8004349
AACGCAG367550.031.1818186
ACTGTGC69300.030.852588
TAGTACT68400.030.8471034
TCTAACG33000.030.6447932
GGGCACC61200.030.27190294
CTAGGCA74600.029.9211944
TAACGCC33650.029.8849724
Produced by FastQC (version 0.11.5)