FastQCFastQC Report
Mon 6 Jun 2016
ERR1211359_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211359_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6020811
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307830.5112766369846189No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256320.4257233784618052No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA224600.3730394460148309No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC200590.3331610974003336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172520.28653947117755396No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT164430.27310274313543476No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC163860.27215602682097145No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC160520.2666086013993796No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA151950.25237463856613335No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC127230.2113170468230941No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG119760.19891008038618055No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT119500.19847824487432011No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC108990.181022124760269No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC92780.15409884150158507No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC91970.1527535077915583No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT87120.14469811458954618No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA86060.1429375544258074No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG84490.14032993229649626No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG84480.14031332323834778No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT81710.13571261413121918No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC77170.12817210173180987No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT76350.12681015896363462No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT74250.12332225675245412No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT71540.11882120199421639No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA71310.11843919365680138No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC71300.1184225845986529No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT70910.11777483133086225No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC70020.11629662515564762No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA69800.11593122587638109No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA69690.11574852623674785No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG68480.11373883020078192No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC63650.10571665511506673No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT63250.10505229278912759No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA62090.10312564204390405No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC61000.10131525470571988No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143250.055.724911
GTATCAA270250.041.728021
GTACTAG56200.035.5723881
CAACGCA333600.032.710435
TAGGCAT64650.032.437075
ATCAACG336950.032.4337233
TACCTGG15050.032.1112
TATTCTC34650.031.8175855
GGGCACC56900.031.50137194
ACCTGGG15200.031.1796043
TATCAAC363500.030.7461282
AACGCAG368650.030.134926
TCTCTGC37450.030.0650548
CTGTGCG65800.029.9428779
CATGGGT19800.029.8605754
TCAAGAC8700.029.6644463
ACTGTGC68600.029.4067428
TAGTACT66900.029.388724
CTAGGCA76300.028.3510514
TACCCCG3000.028.1484095
Produced by FastQC (version 0.11.5)