##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211359_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6020811 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10703275688275 33.0 31.0 34.0 30.0 34.0 2 32.24136881227462 33.0 31.0 34.0 30.0 34.0 3 32.29626656608221 34.0 31.0 34.0 30.0 34.0 4 35.94189337615813 37.0 35.0 37.0 35.0 37.0 5 35.86437923396034 37.0 35.0 37.0 35.0 37.0 6 35.88405449033361 37.0 35.0 37.0 35.0 37.0 7 35.80487246651656 37.0 35.0 37.0 35.0 37.0 8 35.794153976931014 37.0 35.0 37.0 33.0 37.0 9 37.393689820191994 39.0 37.0 39.0 34.0 39.0 10-11 37.34389029982838 39.0 37.0 39.0 34.0 39.0 12-13 37.264327181172106 39.0 37.0 39.0 33.5 39.0 14-15 38.44118715900565 40.0 38.0 41.0 33.5 41.0 16-17 38.45213144873672 40.0 38.0 41.0 34.0 41.0 18-19 38.51286537644181 40.0 38.0 41.0 34.0 41.0 20-21 38.497023241553336 40.0 38.0 41.0 34.0 41.0 22-23 38.367675799821654 40.0 38.0 41.0 34.0 41.0 24-25 38.27373787351903 40.0 38.0 41.0 34.0 41.0 26-27 38.141884540139195 40.0 37.0 41.0 33.0 41.0 28-29 38.06121459384791 40.0 37.0 41.0 33.0 41.0 30-31 38.03142101620529 40.0 37.0 41.0 33.0 41.0 32-33 37.922820198142745 40.0 37.0 41.0 33.0 41.0 34-35 37.78259257764444 40.0 36.5 41.0 33.0 41.0 36-37 37.63322665335285 40.0 36.0 41.0 33.0 41.0 38-39 37.49003481424678 40.0 36.0 41.0 32.5 41.0 40-41 37.35536815555247 40.0 36.0 41.0 32.0 41.0 42-43 37.21482014632248 39.0 36.0 41.0 31.5 41.0 44-45 36.97423038192031 39.0 35.5 41.0 31.0 41.0 46-47 36.63768460760519 39.0 35.0 41.0 30.5 41.0 48-49 35.004511269329 39.0 34.5 40.0 22.5 41.0 50-51 35.67232296446443 38.0 34.5 40.0 27.0 41.0 52-53 35.742050946292785 38.0 35.0 40.0 28.0 41.0 54-55 35.55246386242651 38.0 35.0 40.0 28.0 41.0 56-57 35.29851576473668 38.0 34.0 40.0 27.0 41.0 58-59 35.02393705764888 37.0 34.0 40.0 27.0 41.0 60-61 34.7741651415399 37.0 34.0 40.0 26.0 41.0 62-63 34.44857935251579 36.5 33.5 39.5 26.0 41.0 64-65 34.13052411045622 36.0 33.0 39.0 26.0 41.0 66-67 33.82600799128224 35.0 33.0 39.0 25.0 40.0 68-69 33.515262146577925 35.0 33.0 38.5 25.0 40.0 70-71 33.25491906322919 35.0 32.0 38.0 24.5 40.0 72-73 32.94283652152509 35.0 32.0 37.5 24.0 40.0 74-75 32.61184813806645 35.0 32.0 37.0 23.5 39.0 76-77 31.691096432025518 34.0 30.5 36.0 22.5 39.0 78-79 32.1527261858909 35.0 31.5 36.0 23.5 39.0 80-81 32.060971354191324 35.0 31.5 36.0 23.0 37.5 82-83 31.8103826046026 34.5 31.0 35.0 23.0 37.0 84-85 31.53228078410035 34.0 31.0 35.0 22.5 37.0 86-87 31.291046339106146 34.0 31.0 35.0 21.0 36.0 88-89 31.069441060348847 34.0 31.0 35.0 20.0 36.0 90-91 30.85520314455976 34.0 30.5 35.0 20.0 36.0 92-93 30.64551719029214 34.0 30.0 35.0 20.0 35.0 94-95 30.46712286766683 34.0 30.0 35.0 19.0 35.0 96-97 30.298271943763055 34.0 29.5 35.0 19.0 35.0 98-99 30.09955319972675 34.0 29.0 35.0 18.0 35.0 100 27.59686959115641 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2975215946214327 1101 2 -0.2819899092000746 1101 3 -0.2906232011211749 1101 4 -0.22455146380361413 1101 5 -0.18789219700405368 1101 6 -0.2619412531307006 1101 7 -0.21688196175512076 1101 8 -0.2084562508414507 1101 9 -0.3254481091686827 1101 10-11 -0.29623639235470733 1101 12-13 -0.38585436483546687 1101 14-15 -0.4757340402704813 1101 16-17 -0.5241490646163598 1101 18-19 -0.5320995527517525 1101 20-21 -0.5098467944259468 1101 22-23 -0.6321668178208739 1101 24-25 -0.5189845916344922 1101 26-27 -0.5974718375597234 1101 28-29 -0.6013866907849703 1101 30-31 -0.5252767055991896 1101 32-33 -0.4660936488260532 1101 34-35 -0.44488869747161175 1101 36-37 -0.5347607548641875 1101 38-39 -0.5230054012699412 1101 40-41 -0.5047122442843488 1101 42-43 -0.5844793288872125 1101 44-45 -0.543206608541567 1101 46-47 -0.5724747032374182 1101 48-49 0.6418986260972801 1101 50-51 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1105 68-69 -0.6323371212300728 1105 70-71 -0.7497935973801546 1105 72-73 -0.6898023728586296 1105 74-75 -0.8289904975139137 1105 76-77 -0.8473333370386875 1105 78-79 -0.6477883225937333 1105 80-81 -0.5935400637520161 1105 82-83 -0.5721221761020914 1105 84-85 -0.6376332950485661 1105 86-87 -0.5861049777339495 1105 88-89 -0.6760939529105165 1105 90-91 -0.5904117728840177 1105 92-93 -0.5363769083977523 1105 94-95 -0.5478557380660973 1105 96-97 -0.5903728854479269 1105 98-99 -0.7477446991819114 1105 100 -0.8031239038689009 1106 1 -0.32762267278855006 1106 2 -0.24180122995478825 1106 3 -0.3153615209774223 1106 4 -0.25716714215042913 1106 5 -0.22915005863927007 1106 6 -0.26747921359790894 1106 7 -0.31577908664281296 1106 8 -0.3527105185862851 1106 9 -0.47373876505368173 1106 10-11 -0.38380097007258485 1106 12-13 -0.4361783765156133 1106 14-15 -0.573647899210286 1106 16-17 -0.5607814770152757 1106 18-19 -0.6404194089961379 1106 20-21 -0.5608676839138198 1106 22-23 -0.5728636956285982 1106 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74-75 1.2260992200707292 2209 76-77 1.1727790699930871 2209 78-79 1.0840582993441643 2209 80-81 1.1333708557882147 2209 82-83 1.1287388526595379 2209 84-85 1.1226788961409078 2209 86-87 1.145980761070856 2209 88-89 1.151944872699307 2209 90-91 1.0914532476600094 2209 92-93 1.092465344683852 2209 94-95 1.0395934219692684 2209 96-97 1.2126480616577062 2209 98-99 1.258540212660808 2209 100 1.4515380298701146 2210 1 0.540604071397496 2210 2 0.4591829893143142 2210 3 0.4360462863315462 2210 4 0.30102222662033995 2210 5 0.367660572589962 2210 6 0.37360052062135907 2210 7 0.4065464947525328 2210 8 0.39433267078248235 2210 9 0.585650769830039 2210 10-11 0.5794361561732657 2210 12-13 0.6205886500624587 2210 14-15 0.7638417187299211 2210 16-17 0.7847127090312327 2210 18-19 0.7408243617679844 2210 20-21 0.6950936948237256 2210 22-23 0.6988161216471553 2210 24-25 0.7715789648543563 2210 26-27 0.7878982077817938 2210 28-29 0.8212700979068188 2210 30-31 0.8038452333255677 2210 32-33 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0.9842353220999307 2210 100 1.2563020517579382 2211 1 0.518149310851534 2211 2 0.4595046420872819 2211 3 0.43243171594924945 2211 4 0.29193853999973385 2211 5 0.37149849526081624 2211 6 0.3883566759726449 2211 7 0.409680305703084 2211 8 0.4214656426757628 2211 9 0.573434036582313 2211 10-11 0.5671092329656844 2211 12-13 0.5863616103380309 2211 14-15 0.7190768670766019 2211 16-17 0.6719100581877555 2211 18-19 0.6804131939837035 2211 20-21 0.6516721203514422 2211 22-23 0.6770455579501373 2211 24-25 0.6927889822469666 2211 26-27 0.7711943440419802 2211 28-29 0.7902343632391435 2211 30-31 0.798937406483887 2211 32-33 0.7798618464638594 2211 34-35 0.7513859485136507 2211 36-37 0.79118415719023 2211 38-39 0.777402494264706 2211 40-41 0.7396287563826149 2211 42-43 0.6419897840502387 2211 44-45 0.7236279016255054 2211 46-47 0.7962077390646556 2211 48-49 2.0013301079156136 2211 50-51 1.1685732247311904 2211 52-53 0.8822832775016778 2211 54-55 0.8288122671798135 2211 56-57 0.7414150869446701 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0.28090313434726966 2311 5 0.2607859372644654 2311 6 0.3193003136251633 2311 7 0.3651677472878916 2311 8 0.20065439010119945 2311 9 0.3831638633812062 2311 10-11 0.3334166651894108 2311 12-13 0.4320319622794102 2311 14-15 0.4551470103305917 2311 16-17 0.4404842039882979 2311 18-19 0.5803081042313707 2311 20-21 0.5791043676308121 2311 22-23 0.6446327841461112 2311 24-25 0.5644495783410619 2311 26-27 0.6099023199209412 2311 28-29 0.6242710217488892 2311 30-31 0.5415366874390983 2311 32-33 0.4855909067842461 2311 34-35 0.6558462225192372 2311 36-37 0.6658294514624785 2311 38-39 0.6790276747588777 2311 40-41 0.6396557977860624 2311 42-43 0.7140849666490823 2311 44-45 0.7586513619408635 2311 46-47 0.7917832759399133 2311 48-49 -6.055591326467233 2311 50-51 -1.116052271464163 2311 52-53 0.3359078595771834 2311 54-55 0.7374334618810607 2311 56-57 0.8065540452040523 2311 58-59 0.8263685612531901 2311 60-61 0.733216605224321 2311 62-63 0.5871073595391678 2311 64-65 0.6684420108124769 2311 66-67 0.7421834259688254 2311 68-69 0.8107584439594788 2311 70-71 0.7984544163990037 2311 72-73 0.3584070177589922 2311 74-75 0.665380265822364 2311 76-77 0.7734830021035641 2311 78-79 0.6731374686463028 2311 80-81 0.6550210947368456 2311 82-83 0.7177913159387614 2311 84-85 0.7324910754929341 2311 86-87 0.801387852222728 2311 88-89 0.7854271975288754 2311 90-91 0.8163386050287613 2311 92-93 0.9050224463775756 2311 94-95 0.8950772929370139 2311 96-97 0.9256172618826 2311 98-99 0.9726729697062773 2311 100 1.2903956446005935 2312 1 0.05588790300463131 2312 2 0.20141767217715767 2312 3 0.1965284823799749 2312 4 0.014527712642255608 2312 5 0.20599850607450776 2312 6 0.1410777362090414 2312 7 0.3564095150023121 2312 8 0.35998798737308846 2312 9 0.33337933130354713 2312 10-11 0.29129293205936335 2312 12-13 0.3337638133450582 2312 14-15 0.3321777051207562 2312 16-17 0.4898097392009362 2312 18-19 0.3838507773392621 2312 20-21 0.4295366723057512 2312 22-23 0.6417553449958788 2312 24-25 0.7091112456184376 2312 26-27 0.6083795840012911 2312 28-29 0.7921007554096136 2312 30-31 0.8230089907381881 2312 32-33 0.8028821636792856 2312 34-35 0.41689917589337 2312 36-37 0.7409635792789331 2312 38-39 0.8314464855753059 2312 40-41 0.5579925251164752 2312 42-43 0.5677520980160793 2312 44-45 0.7324162383179456 2312 46-47 0.8563450326475319 2312 48-49 -4.682922286459483 2312 50-51 -0.7865799169216885 2312 52-53 0.4899368886062092 2312 54-55 0.7116078466445046 2312 56-57 0.8278628715619689 2312 58-59 0.8954278905684845 2312 60-61 0.9061812072737823 2312 62-63 0.6858634613632901 2312 64-65 0.9367283546598273 2312 66-67 0.874857562236933 2312 68-69 0.7946672300246505 2312 70-71 0.9251964283969727 2312 72-73 0.9872533430464117 2312 74-75 0.6201556934238752 2312 76-77 0.8657837530649068 2312 78-79 0.5246375486702206 2312 80-81 0.6989275537905613 2312 82-83 0.6975459653988807 2312 84-85 0.818937795705434 2312 86-87 0.9484743672089699 2312 88-89 0.9411883233110387 2312 90-91 0.6818352377063199 2312 92-93 0.8872612595245251 2312 94-95 1.0193025876566963 2312 96-97 1.0204246393863272 2312 98-99 1.0609973565054212 2312 100 0.5700864504134167 2313 1 0.06522495177691923 2313 2 0.1721931418379583 2313 3 0.26772054325035555 2313 4 0.05500323527053297 2313 5 0.12995273891080217 2313 6 0.27167965910528835 2313 7 0.1148958843884742 2313 8 0.22876771349449143 2313 9 0.44503006461028605 2313 10-11 0.23375148399131263 2313 12-13 -0.019199709904121676 2313 14-15 0.051514015207857256 2313 16-17 0.033732572810905026 2313 18-19 0.3775941649154646 2313 20-21 0.3352014685443834 2313 22-23 0.4708067438681809 2313 24-25 0.21609326731467604 2313 26-27 0.38041282687689204 2313 28-29 0.32588504980527944 2313 30-31 0.5172014103985134 2313 32-33 0.4167394211080122 2313 34-35 0.3608563736826298 2313 36-37 -0.3405918602353424 2313 38-39 0.15017637597757272 2313 40-41 0.401573810442315 2313 42-43 0.500522555510706 2313 44-45 0.5174575740513845 2313 46-47 0.3076844867421684 2313 48-49 -5.213466663397256 2313 50-51 -1.2921795420644884 2313 52-53 0.06856150105179637 2313 54-55 0.25049689817273446 2313 56-57 0.22942311423196315 2313 58-59 0.347671468092301 2313 60-61 0.6715661984163646 2313 62-63 0.7157141720669458 2313 64-65 0.8400695264528153 2313 66-67 0.9075470196675539 2313 68-69 0.9022553650385987 2313 70-71 0.7754486025823439 2313 72-73 0.4987276429294454 2313 74-75 0.7131464561146217 2313 76-77 0.7903466352578938 2313 78-79 0.7831495337521872 2313 80-81 -0.22699559844121708 2313 82-83 0.3077227324223415 2313 84-85 0.6048917650659966 2313 86-87 0.7616230144959779 2313 88-89 0.7314208797881143 2313 90-91 0.7823813541285496 2313 92-93 0.8695681324965427 2313 94-95 0.8076525181453995 2313 96-97 0.7978824526330968 2313 98-99 0.9478749546688512 2313 100 0.9907920002879429 2314 1 0.07856608801836273 2314 2 0.18786529948118869 2314 3 0.22080099446102963 2314 4 0.03621130577052867 2314 5 0.19823157989510065 2314 6 0.2904371099920482 2314 7 0.12219641767995171 2314 8 0.25843192686451744 2314 9 0.45292550007600596 2314 10-11 0.3249667841472572 2314 12-13 0.20971010639917154 2314 14-15 0.24175581631050846 2314 16-17 0.46981110223543965 2314 18-19 0.5395126618148964 2314 20-21 0.5733732588609897 2314 22-23 0.37184323109022444 2314 24-25 0.434088598927147 2314 26-27 0.5048005601710486 2314 28-29 0.5661049782767691 2314 30-31 0.4924659273139085 2314 32-33 0.4894595332428153 2314 34-35 0.38012657915178494 2314 36-37 0.4660239845658154 2314 38-39 0.516904388974254 2314 40-41 0.28397302102398925 2314 42-43 0.2903803333181614 2314 44-45 0.523999076951597 2314 46-47 0.6006177902441223 2314 48-49 -6.45551166257912 2314 50-51 -1.6255525021494392 2314 52-53 -0.8405103814303985 2314 54-55 -0.37862287869303657 2314 56-57 -0.09674824030167883 2314 58-59 0.36590134610646885 2314 60-61 0.5472636598554175 2314 62-63 0.42039124186901944 2314 64-65 0.5736401333098016 2314 66-67 0.6238501442814481 2314 68-69 0.633025899432063 2314 70-71 0.4806278245845448 2314 72-73 0.6709421800478026 2314 74-75 0.7069188505165656 2314 76-77 0.5607225773672191 2314 78-79 0.37161042682340195 2314 80-81 0.4793614897939804 2314 82-83 0.4442185949887545 2314 84-85 0.5245932036506566 2314 86-87 0.5290986624100675 2314 88-89 0.4016155795987757 2314 90-91 0.6004388808181709 2314 92-93 0.6356548683319296 2314 94-95 0.6435991856580294 2314 96-97 0.6776338643361264 2314 98-99 0.7783520451747954 2314 100 -0.5169981627049012 2315 1 0.14773486989267326 2315 2 0.22429328845711183 2315 3 0.30425829630297585 2315 4 0.1284605316730918 2315 5 0.1765170478338831 2315 6 0.2601454815362345 2315 7 0.302783046018817 2315 8 0.21982896404135488 2315 9 0.39446652461886345 2315 10-11 0.2208471097722864 2315 12-13 0.34017950101826955 2315 14-15 0.22202238086648407 2315 16-17 0.34581924366761996 2315 18-19 0.3869773364358693 2315 20-21 0.26387857773105594 2315 22-23 0.35659661683392585 2315 24-25 0.42350844727744175 2315 26-27 0.45374721096457904 2315 28-29 0.4942759346409744 2315 30-31 0.4554040212960686 2315 32-33 0.5488062103302553 2315 34-35 0.5595258884124874 2315 36-37 0.4510988254110586 2315 38-39 0.5553811652445617 2315 40-41 0.5460712223110988 2315 42-43 0.5832857978532431 2315 44-45 0.5575861747092716 2315 46-47 0.4704664026759744 2315 48-49 -4.980856870917997 2315 50-51 -1.081538075989748 2315 52-53 0.06778000250096028 2315 54-55 0.03784458743742647 2315 56-57 0.38963214137937996 2315 58-59 0.5110912348896903 2315 60-61 0.40740665515074426 2315 62-63 0.09968206860266804 2315 64-65 0.3132058684785406 2315 66-67 -0.3011331598946825 2315 68-69 0.3501185717398201 2315 70-71 0.4236216277722349 2315 72-73 0.44835319412956665 2315 74-75 0.5728809655145 2315 76-77 0.5713701575616206 2315 78-79 0.453292506881084 2315 80-81 0.3609152862388143 2315 82-83 0.3520251831447183 2315 84-85 0.42248269423444995 2315 86-87 0.40611333215134593 2315 88-89 0.379548015551741 2315 90-91 0.5039903930807306 2315 92-93 0.5644570800849813 2315 94-95 0.22180304973305454 2315 96-97 0.3926553672667694 2315 98-99 0.3392736546522137 2315 100 0.38791617642680265 2316 1 0.2074772705060326 2316 2 0.2390610973974745 2316 3 0.17206461009309493 2316 4 -0.06359141004300994 2316 5 0.11628151007553811 2316 6 0.10495198045612142 2316 7 0.23968660658088936 2316 8 0.29900542778658945 2316 9 0.46251088053747225 2316 10-11 0.36570627505683007 2316 12-13 0.23912542781020107 2316 14-15 0.41760541704391585 2316 16-17 0.39152306650612445 2316 18-19 0.01251428617174355 2316 20-21 0.38402260123341847 2316 22-23 0.5436691985753015 2316 24-25 0.5176760580424542 2316 26-27 0.5521775874509274 2316 28-29 0.5944479831245033 2316 30-31 0.566225455498703 2316 32-33 0.5125583289472573 2316 34-35 0.49763174106045227 2316 36-37 0.3875966161138038 2316 38-39 0.5041318209951626 2316 40-41 0.10041520151879979 2316 42-43 0.10705370685732163 2316 44-45 0.3574644437514678 2316 46-47 0.3812462521138187 2316 48-49 -5.004242756348141 2316 50-51 -1.0742230297957818 2316 52-53 0.17291194971779333 2316 54-55 0.5038453225605295 2316 56-57 0.45413651533802124 2316 58-59 0.2537740064974088 2316 60-61 0.3686177426861761 2316 62-63 0.27329312515882265 2316 64-65 0.28930637291551164 2316 66-67 0.435760140367627 2316 68-69 0.12539560975236697 2316 70-71 -0.34024001339485466 2316 72-73 -0.11406362939749215 2316 74-75 0.09413621201522915 2316 76-77 0.07260317896755453 2316 78-79 0.16971424757545606 2316 80-81 0.12354912343777613 2316 82-83 0.0941212806520042 2316 84-85 0.2591824652464645 2316 86-87 0.2880743166671813 2316 88-89 0.19032789276301187 2316 90-91 0.15142683377719024 2316 92-93 0.23663052422320519 2316 94-95 0.4375137508606777 2316 96-97 0.49665548756541256 2316 98-99 0.5415280877087589 2316 100 -0.24275381956033115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 7.0 11 6.0 12 33.0 13 143.0 14 560.0 15 1864.0 16 4757.0 17 10728.0 18 18521.0 19 27480.0 20 40443.0 21 57827.0 22 67524.0 23 61405.0 24 54501.0 25 55188.0 26 62872.0 27 76479.0 28 94563.0 29 115184.0 30 141578.0 31 177188.0 32 224313.0 33 295530.0 34 427561.0 35 590472.0 36 928659.0 37 1282987.0 38 1026987.0 39 175443.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.80615979651206 22.941553705497398 13.986024326178804 22.26626217181174 2 14.642042689459181 21.279238758348296 41.88084754405226 22.197871008140265 3 17.30176190783854 29.467514549139047 30.157422523208282 23.073301019814135 4 11.032267732201346 17.981113694614802 41.96167450910292 29.02494406408093 5 11.32067229805032 40.408322374070345 35.56755584241383 12.703449485465509 6 26.796128760083775 41.26067755003064 18.552071897302856 13.391121792582725 7 25.468471097488727 33.561253977663135 24.52029287094143 16.44998205390671 8 26.017029878276148 35.513034841240774 20.43558817691607 18.034347103567004 9 26.84341409604186 14.683477987299154 22.819713336486036 35.65339458017295 10-11 24.700701441549416 27.29155233971084 27.883638633364917 20.12410758537483 12-13 24.906920661516246 25.54079774555045 28.730587415685065 20.82169417724824 14-15 22.73245702689725 26.35611939341626 26.586553848898177 24.32486973078831 16-17 19.936734221195703 29.11557045540781 28.286476667806326 22.661218655590158 18-19 19.37926287154576 28.596910432060675 31.57110352021601 20.452723176177557 20-21 21.14533032919438 27.69325926460492 30.84602730415844 20.31538310204226 22-23 21.66179009218142 27.512276004288804 30.401888257032027 20.42404564649775 24-25 20.886297543636566 27.674087427756827 31.51253211569006 19.92708291291655 26-27 20.636515896205292 27.939522402973154 30.586874718476327 20.837086982345227 28-29 19.92084055833483 27.852031969385926 31.325214756940984 20.901912715338263 30-31 20.841978943259747 27.73448824979082 30.94736230174961 20.47617050519982 32-33 21.277278548932397 28.376177353371663 29.96005384844548 20.386490249250464 34-35 21.066950334887476 27.914690553537604 31.10814252882843 19.91021658274649 36-37 20.884152672486174 27.40782247638768 30.609876923864103 21.098147927262048 38-39 20.936606373858602 27.656596047489007 31.34765851313734 20.05913906551505 40-41 20.380455793195782 26.73161459346795 32.16156490254033 20.726364710795945 42-43 20.455978252248634 26.932583249736798 30.99563664685126 21.615801851163308 44-45 20.367631156600577 28.168815692913547 30.669477625997345 20.794075524488534 46-47 20.6087265343494 27.78294659402971 30.09046729642616 21.51785957519473 48-49 21.596645167623947 27.730344244680268 29.744501068452216 20.928509519243573 50-51 21.25239815851992 27.36494685885048 29.30968750555466 22.072967477074943 52-53 20.293563801860216 28.327529729780466 29.299101389035748 22.079805079323574 54-55 20.721099997292463 28.314967211401953 28.931853634236415 22.03207915706917 56-57 21.547110543738725 27.711067412991497 28.593591513975404 22.148230529294374 58-59 20.3235240402276 27.974486962390294 29.702608402296597 21.99938059508551 60-61 20.68578763518306 29.079883567684277 28.398170349324353 21.83615844780831 62-63 21.065421493717533 28.12689123597667 28.510372745455697 22.297314524850098 64-65 20.121523959256006 28.040848317074325 28.788476578982518 23.049151144687155 66-67 20.827618816130915 27.61055898237373 28.6340051307296 22.92781707076576 68-69 20.197237810055228 27.849859823724042 28.886671661381897 23.06623070483883 70-71 20.51557856784599 27.178026511500946 29.284976290034603 23.02141863061846 72-73 21.071986083677853 27.508409674608288 29.045139250854714 22.37446499085915 74-75 21.0114669083183 27.92901076228484 28.730150385288024 22.32937194410884 76-77 20.663779585110767 27.777719637470266 28.744011548326228 22.814489229092747 78-79 20.38076990317347 27.647962872297533 28.730117797236932 23.24114942729207 80-81 19.991583970078995 28.331600548013604 28.869872680764637 22.806942801142764 82-83 21.124779358779154 27.069833091747874 29.875743236576223 21.92964431289674 84-85 21.133519615394743 27.493075890959624 28.525717781098724 22.84768671254691 86-87 19.85692713726936 28.356173862077664 29.177022452725826 22.60987654792715 88-89 20.929803476916824 27.0777053169734 28.878368720591673 23.114122485518106 90-91 20.59272411512595 26.972467447203925 29.3062879172108 23.128520520459325 92-93 20.971585168290904 27.428512339893263 28.718394453827507 22.881508037988326 94-95 19.488501396993936 26.98110117438514 29.586879801050657 23.94351762757027 96-97 20.164225082357117 27.05228409529084 29.07671580064939 23.706775021702647 98-99 19.538056151381568 27.685484155642442 28.967642667740883 23.808817025235108 100 20.086590674139867 27.062277296847704 28.217750058069385 24.633381970943045 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1861.0 1 1254.0 2 807.0 3 970.0 4 828.0 5 788.0 6 1334.0 7 2697.0 8 4107.5 9 4766.0 10 4474.0 11 4098.0 12 4607.5 13 5409.0 14 6416.0 15 7799.0 16 9100.5 17 10213.5 18 11267.5 19 12220.0 20 13116.5 21 13710.0 22 14604.5 23 16188.0 24 17440.0 25 18927.5 26 22738.0 27 26722.5 28 30240.5 29 42166.5 30 65048.5 31 86473.0 32 117769.5 33 155980.5 34 186376.5 35 216187.0 36 248407.0 37 286512.5 38 290754.0 39 264653.0 40 230785.0 41 193948.5 42 173371.0 43 172717.0 44 183331.5 45 212415.0 46 233345.5 47 233048.0 48 237283.5 49 230042.0 50 213440.0 51 205966.5 52 200818.0 53 188982.0 54 177132.5 55 151244.5 56 129790.0 57 103791.5 58 76711.5 59 74340.5 60 59859.0 61 36678.0 62 25010.0 63 20979.0 64 14655.5 65 5244.0 66 1960.0 67 1297.5 68 1014.0 69 792.0 70 633.5 71 470.5 72 390.0 73 380.5 74 278.5 75 173.5 76 126.0 77 87.5 78 64.5 79 51.0 80 24.5 81 12.0 82 6.5 83 4.0 84 3.5 85 3.5 86 2.0 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02958073256244051 2 0.002009696035965919 3 1.3287246518782934E-4 4 0.023634689745285143 5 4.816626863058814E-4 6 1.6609058148478667E-5 7 3.3218116296957334E-5 8 0.0035875565600713925 9 0.009666471842414585 10-11 0.0011128068959480708 12-13 1.0795887796511135E-4 14-15 1.6609058148478668E-4 16-17 3.4048569204381274E-4 18-19 2.4913587222718002E-5 20-21 1.0795887796511135E-4 22-23 3.4048569204381274E-4 24-25 0.0 26-27 4.9827174445436004E-5 28-29 8.47061965572412E-4 30-31 0.002881671588761049 32-33 0.009658167313340345 34-35 0.018311486608697733 36-37 0.02134263972079509 38-39 0.021101808377642146 40-41 0.004907976682875447 42-43 0.005422857485478285 44-45 0.009384117853890447 46-47 0.01220765773913182 48-49 1.1961677587952853 50-51 0.018070655265544792 52-53 0.018793149295003614 54-55 0.00960003560982067 56-57 0.007731516568116821 58-59 0.008578578533689233 60-61 0.0052733759621419775 62-63 0.007349508230701811 64-65 0.019515643324462435 66-67 0.029630559736885942 68-69 0.029605646149663226 70-71 0.026773801735347613 72-73 0.02332742216953829 74-75 0.009541903906300995 76-77 0.014798670810294495 78-79 0.008445706068501403 80-81 0.0026159266583853902 82-83 0.014233962833246218 84-85 0.023991784495477435 86-87 0.023759257681398735 88-89 0.03324302988418005 90-91 0.021292812546349654 92-93 0.013345378222302612 94-95 0.0063778783290158085 96-97 0.003861606019521291 98-99 0.015338465200120052 100 0.03569286596108066 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6020811.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.44115846701015 #Duplication Level Percentage of deduplicated Percentage of total 1 75.15793149039497 16.11473119138251 2 13.122923517383825 5.627413653733617 3 4.681367014069996 3.011217959727268 4 2.192714635576116 1.8805736789727963 5 1.1868976905390491 1.2724230733488064 6 0.7035206080653164 0.9050578105401472 7 0.4583120403491018 0.6878718759124689 8 0.32236438060021727 0.5529492614855065 9 0.24707224017209653 0.4767763548896202 >10 1.2640248854021203 5.206492542535739 >50 0.2049020056275263 3.1376564949124233 >100 0.3126317704525415 15.336366146332786 >500 0.07759476796043539 11.625672930148955 >1k 0.06295778633791031 24.548726917953196 >5k 0.003565418600358557 5.033090130694015 >10k+ 0.001219748468543717 4.582979977430226 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30783 0.5112766369846189 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25632 0.4257233784618052 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 22460 0.3730394460148309 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 20059 0.3331610974003336 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17252 0.28653947117755396 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 16443 0.27310274313543476 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 16386 0.27215602682097145 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 16052 0.2666086013993796 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 15195 0.25237463856613335 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 12723 0.2113170468230941 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 11976 0.19891008038618055 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 11950 0.19847824487432011 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 10899 0.181022124760269 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 9278 0.15409884150158507 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 9197 0.1527535077915583 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 8712 0.14469811458954618 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 8606 0.1429375544258074 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 8449 0.14032993229649626 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 8448 0.14031332323834778 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 8171 0.13571261413121918 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 7717 0.12817210173180987 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 7635 0.12681015896363462 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 7425 0.12332225675245412 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 7154 0.11882120199421639 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 7131 0.11843919365680138 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 7130 0.1184225845986529 No Hit GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT 7091 0.11777483133086225 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 7002 0.11629662515564762 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 6980 0.11593122587638109 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 6969 0.11574852623674785 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 6848 0.11373883020078192 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 6365 0.10571665511506673 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 6325 0.10505229278912759 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 6209 0.10312564204390405 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 6100 0.10131525470571988 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.6609058148478667E-5 0.0 14-15 0.0 0.0 0.0 2.4913587222718E-5 0.0 16-17 0.0 0.0 0.0 3.3218116296957334E-5 0.0 18-19 0.0 0.0 0.0 5.813170351967534E-5 0.0 20-21 0.0 0.0 0.0 1.9100416870750466E-4 0.0 22-23 0.0 0.0 0.0 5.48098918899796E-4 0.0 24-25 0.0 0.0 0.0 0.0013204201228040541 0.0 26-27 0.0 0.0 0.0 0.0027404945944989804 0.0 28-29 0.0 0.0 0.0 0.008038784143863675 0.0 30-31 0.0 0.0 0.0 0.028575884544457548 0.0 32-33 8.304529074239333E-6 0.0 0.0 0.07125285945697349 0.0 34-35 1.6609058148478667E-5 0.0 0.0 0.13314651464727925 0.0 36-37 1.6609058148478667E-5 0.0 0.0 0.23300847676500724 0.0 38-39 1.6609058148478667E-5 0.0 0.0 0.37614533988859644 0.0 40-41 1.6609058148478667E-5 0.0 0.0 0.5269306742895601 0.0 42-43 1.6609058148478667E-5 0.0 0.0 0.6750087322123215 0.0 44-45 1.6609058148478667E-5 0.0 0.0 0.8463726896592503 0.0 46-47 1.6609058148478667E-5 0.0 0.0 1.0557215630917496 0.0 48-49 1.6609058148478667E-5 0.0 0.0 1.289228311601211 0.0 50-51 1.6609058148478667E-5 0.0 0.0 1.5459877415185428 0.0 52-53 1.6609058148478667E-5 0.0 0.0 1.7901658098884021 0.0 54-55 1.6609058148478667E-5 0.0 0.0 2.0435120783562213 0.0 56-57 1.6609058148478667E-5 0.0 0.0 2.3411464003769593 0.0 58-59 1.6609058148478667E-5 0.0 0.0 2.692444589275431 0.0 60-61 1.6609058148478667E-5 0.0 0.0 3.052445924643707 0.0 62-63 3.3218116296957334E-5 0.0 0.0 3.411940683738453 0.0 64-65 4.9827174445436004E-5 0.0 0.0 3.7714271383041256 0.0 66-67 8.304529074239334E-5 0.0 0.0 4.165360779469743 0.0 68-69 8.304529074239334E-5 0.0 0.0 4.590926371879137 1.6609058148478667E-5 70-71 8.304529074239334E-5 0.0 0.0 5.060115655515512 1.6609058148478667E-5 72-73 8.304529074239334E-5 0.0 0.0 5.526605967202757 1.6609058148478667E-5 74-75 8.304529074239334E-5 0.0 0.0 5.962386130373466 1.6609058148478667E-5 76-77 9.965434889087201E-5 0.0 0.0 6.444239820848055 1.6609058148478667E-5 78-79 9.965434889087201E-5 0.0 0.0 6.954652786808953 1.6609058148478667E-5 80-81 9.965434889087201E-5 0.0 0.0 7.507742727682367 1.6609058148478667E-5 82-83 9.965434889087201E-5 0.0 0.0 8.071205025369505 1.6609058148478667E-5 84-85 9.965434889087201E-5 0.0 0.0 8.59360142678453 1.6609058148478667E-5 86-87 9.965434889087201E-5 0.0 0.0 9.126552884652915 1.6609058148478667E-5 88 9.965434889087201E-5 0.0 0.0 9.545225717930691 1.6609058148478667E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14325 0.0 55.72491 1 GTATCAA 27025 0.0 41.72802 1 GTACTAG 5620 0.0 35.572388 1 CAACGCA 33360 0.0 32.71043 5 TAGGCAT 6465 0.0 32.43707 5 ATCAACG 33695 0.0 32.433723 3 TACCTGG 1505 0.0 32.111 2 TATTCTC 3465 0.0 31.817585 5 GGGCACC 5690 0.0 31.501371 94 ACCTGGG 1520 0.0 31.179604 3 TATCAAC 36350 0.0 30.746128 2 AACGCAG 36865 0.0 30.13492 6 TCTCTGC 3745 0.0 30.065054 8 CTGTGCG 6580 0.0 29.942877 9 CATGGGT 1980 0.0 29.860575 4 TCAAGAC 870 0.0 29.664446 3 ACTGTGC 6860 0.0 29.406742 8 TAGTACT 6690 0.0 29.38872 4 CTAGGCA 7630 0.0 28.351051 4 TACCCCG 300 0.0 28.148409 5 >>END_MODULE