FastQCFastQC Report
Mon 6 Jun 2016
ERR1211358_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211358_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719850
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383661.410592495909701No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335661.2341121753037851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271870.999577182565215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192020.7059948158905821No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135840.49943930731474157No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA130720.48061473978344393No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA104460.3840652977186242No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58120.21368825486699633No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG57380.2109675165909885No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54760.20133463242458224No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT40720.1497141386473519No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT38440.14133132341857088No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC37530.1379855506737504No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC37500.13787525047337168No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36880.1355957129988786No Hit
GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA28480.10471165689284335No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC28060.10316745408754159No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28030.10305715388716291No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3650.073.453642
TACCTGG44000.068.950652
ACCTGGG44200.066.77183
TAACGCA6050.059.035594
TCACGCA4650.057.6073074
TATAACG6000.056.4433332
ATCACGC4800.055.807083
GTACACG4350.055.147811
CCTGGGG22000.052.1222084
TACGTGG6150.050.4777762
CACGCAG5700.047.818165
GTATCAC5850.047.4398731
GTACGTG6800.046.3461071
ACGTGGG6450.045.173913
TACTTGG8350.044.501232
ACACGGG5300.042.561913
ATAACGC8400.042.5196843
GTACTTG9250.041.6984061
GTACTCG1550.039.451091
GTACCTG77400.039.4412881
Produced by FastQC (version 0.11.5)