FastQCFastQC Report
Mon 6 Jun 2016
ERR1211358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211358_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719850
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323721.1902126955530636No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285051.0480357372649227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244680.8996084342886556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184310.677647664393257No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA125340.46083423718219757No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120070.44145816864900633No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110630.4067503722631763No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54960.20206996709377353No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG53620.19714322481019175No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51470.1892383771163851No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT41560.15280254425795542No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT36990.13600014706693384No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC34290.1260731290328511No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG34030.12511719396290236No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT31390.115410776329577No Hit
GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29170.10724856150155339No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG27910.1026159530856481No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG43600.070.0565262
ACCTGGG44100.066.822563
TATAACG6200.059.027862
TAACGCA6600.058.3004574
CCTGGGG20300.057.5579534
TACGTGG6450.053.830222
GTACGTG6600.053.3324171
ACGTGGG6350.052.4670523
TATCACG3850.051.1849982
GTACACG3350.049.0339851
CTGGGGG13400.044.8088155
CGTGGGA4200.043.5729874
ATCACGC4500.042.7536663
TACTTGG9800.042.1318662
ACACGGG3850.041.4400443
GTACTTG10150.041.152661
ACAACGC2300.040.8041343
GTACCCG2100.040.227881
TCACGCA4800.040.0815664
GTATCAC5400.039.979561
Produced by FastQC (version 0.11.5)