##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211358_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2719850 Sequences flagged as poor quality 0 Sequence length 100 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.077131091788154 33.0 31.0 34.0 30.0 34.0 2 32.12817839219075 33.0 31.0 34.0 30.0 34.0 3 32.20713899663584 34.0 31.0 34.0 30.0 34.0 4 35.86757909443536 37.0 35.0 37.0 35.0 37.0 5 35.823389157490304 37.0 35.0 37.0 35.0 37.0 6 35.89363163409747 37.0 35.0 37.0 35.0 37.0 7 35.83221905619795 37.0 35.0 37.0 35.0 37.0 8 35.82379763589904 37.0 35.0 37.0 35.0 37.0 9 37.41764472305458 39.0 37.0 39.0 34.0 39.0 10-11 37.36164475982132 39.0 37.0 39.0 34.0 39.0 12-13 37.287681857455375 39.0 37.0 39.0 34.0 39.0 14-15 38.42217420078313 40.0 38.0 41.0 34.0 41.0 16-17 38.42459418717944 40.0 38.0 41.0 34.0 41.0 18-19 38.5301152637094 40.0 38.0 41.0 34.0 41.0 20-21 38.53882493519863 40.0 38.0 41.0 34.0 41.0 22-23 38.36120337518613 40.0 37.5 41.0 34.0 41.0 24-25 38.25788646432707 40.0 37.0 41.0 34.0 41.0 26-27 38.11715774766991 40.0 37.0 41.0 34.0 41.0 28-29 37.981251539606966 40.0 37.0 41.0 33.0 41.0 30-31 37.80968288692391 40.0 36.5 41.0 33.0 41.0 32-33 37.52170855010387 40.0 35.5 41.0 33.0 41.0 34-35 37.29145559497766 40.0 35.0 41.0 33.0 41.0 36-37 37.06820817324485 39.5 35.0 41.0 32.0 41.0 38-39 36.92597606485651 39.0 35.0 41.0 31.0 41.0 40-41 36.80489935106716 39.0 35.0 41.0 31.0 41.0 42-43 36.65472948875857 39.0 35.0 41.0 31.0 41.0 44-45 36.41822931411659 39.0 35.0 41.0 30.0 41.0 46-47 36.04177950989944 39.0 35.0 41.0 28.0 41.0 48-49 34.39317830027392 39.0 34.0 41.0 15.0 41.0 50-51 35.1215914480578 38.5 34.5 40.0 23.5 41.0 52-53 35.25643638435943 39.0 35.0 40.0 24.0 41.0 54-55 35.13372299943012 38.5 35.0 40.0 23.0 41.0 56-57 34.967255731014575 38.0 34.5 40.0 23.0 41.0 58-59 34.788055223633656 38.0 34.0 40.0 22.5 41.0 60-61 34.53011857271541 38.0 34.0 40.0 22.0 41.0 62-63 34.19758883761972 38.0 33.5 40.0 21.0 41.0 64-65 33.90702299759178 37.0 33.0 40.0 20.0 41.0 66-67 33.5654078349909 36.5 33.0 39.0 19.5 41.0 68-69 33.191493280879456 36.0 32.5 39.0 19.0 40.0 70-71 32.7959604389948 36.0 32.0 39.0 18.0 40.0 72-73 32.38638895527327 35.0 31.5 38.0 17.0 40.0 74-75 31.970383660863654 35.0 31.0 37.0 15.0 39.5 76-77 30.928498262771846 34.0 29.5 36.0 13.0 39.0 78-79 31.309547769178447 35.0 31.0 36.0 15.0 39.0 80-81 31.14917072632682 35.0 31.0 36.0 13.0 38.0 82-83 30.813706086732722 34.5 30.5 35.5 11.5 37.0 84-85 30.477409048293104 34.0 30.0 35.0 10.0 37.0 86-87 30.19075040902991 34.0 30.0 35.0 10.0 36.0 88-89 29.93678015331728 34.0 29.0 35.0 10.0 36.0 90-91 29.670309943563062 34.0 29.0 35.0 10.0 35.5 92-93 29.43138500284942 34.0 29.0 35.0 10.0 35.0 94-95 29.22879938231888 34.0 29.0 35.0 10.0 35.0 96-97 29.012706766917294 34.0 28.5 35.0 10.0 35.0 98-99 28.784603011195472 33.5 27.0 35.0 10.0 35.0 100 26.76089453462507 31.0 23.0 34.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3332306269488079 1101 2 -0.40972760841528455 1101 3 -0.3241711618226937 1101 4 -0.1968443310324517 1101 5 -0.2249978622223523 1101 6 -0.3266400171664614 1101 7 -0.34848710478915024 1101 8 -0.2753933545634766 1101 9 -0.3991646116206269 1101 10-11 -0.4283635773153378 1101 12-13 -0.494667036188396 1101 14-15 -0.5684903666954213 1101 16-17 -0.5326470446250227 1101 18-19 -0.6025036440512324 1101 20-21 -0.6127502487478438 1101 22-23 -0.6726741258258215 1101 24-25 -0.5134225836225852 1101 26-27 -0.508732873302769 1101 28-29 -0.5406683841024815 1101 30-31 -0.3142567093998352 1101 32-33 -0.2695819963140593 1101 34-35 -0.26318820976507595 1101 36-37 -0.4265962394025209 1101 38-39 -0.21121411236624965 1101 40-41 -0.23729981700407876 1101 42-43 -0.22045521071327556 1101 44-45 -0.228070767066626 1101 46-47 -0.22302645319335568 1101 48-49 1.0325077819610229 1101 50-51 0.1857111015448183 1101 52-53 -0.12892772401299624 1101 54-55 -0.12953436410848695 1101 56-57 -0.13578205369483243 1101 58-59 -0.24802000262102553 1101 60-61 -0.17854991850815338 1101 62-63 -0.10785246445191632 1101 64-65 -0.2757834692804977 1101 66-67 -0.2509581552813316 1101 68-69 -0.47354161994298494 1101 70-71 -0.15563267254730562 1101 72-73 -0.23061892961263197 1101 74-75 -0.467170837066444 1101 76-77 -0.33616250079604626 1101 78-79 -0.16253272019704923 1101 80-81 0.024990380647249566 1101 82-83 0.05936533059048088 1101 84-85 0.028845365171537907 1101 86-87 -0.008174112606823769 1101 88-89 -0.22285094820008666 1101 90-91 -0.25717875342776253 1101 92-93 -0.1373014715859071 1101 94-95 -0.17952921866477567 1101 96-97 -0.4623402840483912 1101 98-99 -0.5091602695902075 1101 100 -0.7877524835486795 1102 1 -0.3575206016470709 1102 2 -0.2864710335042453 1102 3 -0.2963564041331104 1102 4 -0.25255586924871665 1102 5 -0.21157250498320934 1102 6 -0.3703577495362822 1102 7 -0.34431449531505365 1102 8 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-0.16616458075033336 1102 72-73 -0.26736845913579543 1102 74-75 -0.28636402701593866 1102 76-77 -0.19334831704641786 1102 78-79 -0.16211611895074185 1102 80-81 -0.14026441570961268 1102 82-83 0.02232025497334078 1102 84-85 -0.11368932521094521 1102 86-87 -0.12230548196599145 1102 88-89 -0.12153790111545959 1102 90-91 -0.25961689875287774 1102 92-93 -0.3916587973687413 1102 94-95 -0.25238034127814046 1102 96-97 -0.3256758339774315 1102 98-99 -0.3944082314436521 1102 100 -0.13534349701230042 1103 1 -0.24807694722452922 1103 2 -0.25793792186320985 1103 3 -0.286227545483829 1103 4 -0.18346794345729478 1103 5 -0.10610574788941562 1103 6 -0.1508123646011441 1103 7 -0.19544884522426287 1103 8 -0.24547109138898549 1103 9 -0.4027826900532787 1103 10-11 -0.3466889195204601 1103 12-13 -0.4190393249127453 1103 14-15 -0.5224005833800334 1103 16-17 -0.6604551923141173 1103 18-19 -0.5425770319634253 1103 20-21 -0.5990931388181906 1103 22-23 -0.44728060353220656 1103 24-25 -0.4871939612496021 1103 26-27 -0.43200488695836015 1103 28-29 -0.49958854892630455 1103 30-31 -0.38741380302106876 1103 32-33 -0.4080069286340944 1103 34-35 -0.4637555255070538 1103 36-37 -0.3235408016300312 1103 38-39 -0.429538584788709 1103 40-41 -0.3938808100784357 1103 42-43 -0.47891863091616216 1103 44-45 -0.35018607850318517 1103 46-47 -0.2697661816037211 1103 48-49 0.8390218017703219 1103 50-51 -0.04699207424912544 1103 52-53 -0.12640796363811546 1103 54-55 -0.2617104516086215 1103 56-57 -0.34350583246560973 1103 58-59 -0.454234491025737 1103 60-61 -0.6158779461672381 1103 62-63 -0.6301858738912358 1103 64-65 -0.4005967378150217 1103 66-67 -0.39881762014754685 1103 68-69 -0.5295608421398441 1103 70-71 -0.4795575668143428 1103 72-73 -0.5597629547493526 1103 74-75 -0.5606537730178225 1103 76-77 -0.5101286010167492 1103 78-79 -0.3314501669500416 1103 80-81 -0.19241038258703824 1103 82-83 -0.4943972404870678 1103 84-85 -0.30629537653562977 1103 86-87 -0.2540211178363876 1103 88-89 -0.24402471990388364 1103 90-91 -0.296981312763247 1103 92-93 -0.339040275852188 1103 94-95 -0.462134000294963 1103 96-97 -0.5051501169413974 1103 98-99 -0.5352100429031061 1103 100 -0.7743441314606585 1104 1 -0.2319742646690166 1104 2 -0.2949960351499996 1104 3 -0.30118791085733676 1104 4 -0.17879788203674707 1104 5 -0.14287303226129922 1104 6 -0.1606134040257885 1104 7 -0.17436637370344243 1104 8 -0.19735937948809834 1104 9 -0.3989263196401325 1104 10-11 -0.3034861267229374 1104 12-13 -0.347957903315816 1104 14-15 -0.4062055075230333 1104 16-17 -0.4681632084829488 1104 18-19 -0.4093779138994833 1104 20-21 -0.5791540344032597 1104 22-23 -0.480890766795369 1104 24-25 -0.5396381840434543 1104 26-27 -0.5255252817297347 1104 28-29 -0.49094767538962714 1104 30-31 -0.36350231160418645 1104 32-33 -0.3280935473542428 1104 34-35 -0.34673835925161 1104 36-37 -0.3351429854716983 1104 38-39 -0.3081038638059752 1104 40-41 -0.31722324275205693 1104 42-43 -0.32451525016126226 1104 44-45 -0.2529893752641641 1104 46-47 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1105 66-67 -0.5174464686962139 1105 68-69 -0.5716628761376725 1105 70-71 -0.5028162156080214 1105 72-73 -0.4982100697932381 1105 74-75 -0.4463394499877822 1105 76-77 -0.46527494964134775 1105 78-79 -0.2569347727553897 1105 80-81 -0.38022350587221254 1105 82-83 -0.34205333387480863 1105 84-85 -0.39521803602070094 1105 86-87 -0.3375455328974901 1105 88-89 -0.4181345294633658 1105 90-91 -0.4642048905340488 1105 92-93 -0.47789084940110627 1105 94-95 -0.3995729246756028 1105 96-97 -0.4699985036135992 1105 98-99 -0.4331958889510297 1105 100 -0.3681214547640579 1106 1 -0.3475282016637564 1106 2 -0.2223748562199539 1106 3 -0.27752628149788094 1106 4 -0.21675430692008035 1106 5 -0.1857173872524598 1106 6 -0.29634640821056735 1106 7 -0.3300671672924622 1106 8 -0.29222940658354446 1106 9 -0.40820790103582993 1106 10-11 -0.45742191680918154 1106 12-13 -0.5309196472100695 1106 14-15 -0.6323196271803937 1106 16-17 -0.55184366751174 1106 18-19 -0.565254123650142 1106 20-21 -0.6064714181170814 1106 22-23 -0.6196798856187371 1106 24-25 -0.5645015948082417 1106 26-27 -0.5672059409164305 1106 28-29 -0.5028258422397798 1106 30-31 -0.5848927047662045 1106 32-33 -0.5358247389980164 1106 34-35 -0.570837092696884 1106 36-37 -0.6019377161295907 1106 38-39 -0.6620497544530437 1106 40-41 -0.6188789007340958 1106 42-43 -0.5508335123458679 1106 44-45 -0.6920665917349424 1106 46-47 -0.4489054491792359 1106 48-49 0.6768587055775512 1106 50-51 -0.12111129370916274 1106 52-53 -0.3548012503834457 1106 54-55 -0.5646008170571193 1106 56-57 -0.6628203778507284 1106 58-59 -0.7009145231027247 1106 60-61 -0.5249752081674686 1106 62-63 -0.46046101040935383 1106 64-65 -0.5409354554993726 1106 66-67 -0.4778637117196993 1106 68-69 -0.35856454626784284 1106 70-71 -0.44415488609489984 1106 72-73 -0.36128832993551185 1106 74-75 -0.3878130505219275 1106 76-77 -0.4676206772377789 1106 78-79 -0.49818862496356076 1106 80-81 -0.3874128647346673 1106 82-83 -0.2404775475096379 1106 84-85 -0.2451365954610516 1106 86-87 -0.18773842031471588 1106 88-89 -0.19950835682296386 1106 90-91 -0.336422829546386 1106 92-93 -0.18899998868680257 1106 94-95 -0.08991820619865365 1106 96-97 -0.2792987403041387 1106 98-99 -0.3731542518617452 1106 100 -0.5257020571489761 1107 1 -0.27405712197482046 1107 2 -0.21907171386866864 1107 3 -0.32164044516351353 1107 4 -0.16234931416671827 1107 5 -0.18944609397838263 1107 6 -0.1783004713466596 1107 7 -0.2047454847875727 1107 8 -0.30933227673421726 1107 9 -0.34075695134476547 1107 10-11 -0.3262793366846495 1107 12-13 -0.44069698871745544 1107 14-15 -0.5118738685717474 1107 16-17 -0.46702386993585066 1107 18-19 -0.4963764292455082 1107 20-21 -0.4794881275409182 1107 22-23 -0.5166852774390662 1107 24-25 -0.5131693976514953 1107 26-27 -0.5135476931978147 1107 28-29 -0.4443574122912821 1107 30-31 -0.4707782199199144 1107 32-33 -0.547098682012539 1107 34-35 -0.5688011084186755 1107 36-37 -0.5713211697049374 1107 38-39 -0.5161614691161418 1107 40-41 -0.5104337563834704 1107 42-43 -0.4949736715711808 1107 44-45 -0.6809341689487809 1107 46-47 -0.6957789561910133 1107 48-49 0.5820787946248842 1107 50-51 -0.1934000270930838 1107 52-53 -0.5036218147781639 1107 54-55 -0.664699780848018 1107 56-57 -0.7663640042212663 1107 58-59 -0.6706051056842952 1107 60-61 -0.6315730618777096 1107 62-63 -0.7939975908791652 1107 64-65 -0.7559378041329481 1107 66-67 -0.55351881989737 1107 68-69 -0.5854375313610376 1107 70-71 -0.6953131438504201 1107 72-73 -0.6412579984369238 1107 74-75 -0.6148265521539251 1107 76-77 -0.49960776176048327 1107 78-79 -0.44842368905917596 1107 80-81 -0.4745712589919542 1107 82-83 -0.3810548095248052 1107 84-85 -0.3416468672200388 1107 86-87 -0.37125051075918947 1107 88-89 -0.5551446759320093 1107 90-91 -0.4901484248346648 1107 92-93 -0.4214981519349017 1107 94-95 -0.35653478875613587 1107 96-97 -0.4911414741939133 1107 98-99 -0.5689775984839294 1107 100 -0.7075936907942584 1108 1 -0.40711439655029125 1108 2 -0.4276065194270693 1108 3 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0.9187355399592825 2310 76-77 0.8313377579393979 2310 78-79 0.8168296129185961 2310 80-81 0.6345219802746733 2310 82-83 0.6084644673062911 2310 84-85 0.8414269738993063 2310 86-87 0.8986204585882476 2310 88-89 0.9011235137194085 2310 90-91 0.9236187227418142 2310 92-93 0.7273382080817719 2310 94-95 0.7954765092026399 2310 96-97 0.9362097268363421 2310 98-99 0.8807920874004651 2310 100 1.0273636618998943 2311 1 0.26436790422332734 2311 2 0.3255506717840859 2311 3 0.343647276349607 2311 4 0.27573933533922457 2311 5 0.31009917504661644 2311 6 0.3471596752490811 2311 7 0.388531303512508 2311 8 0.26308046059872936 2311 9 0.4859747597092863 2311 10-11 0.3895301090378922 2311 12-13 0.45093113451697064 2311 14-15 0.37424656189310923 2311 16-17 0.3723186672352341 2311 18-19 0.5712784555667625 2311 20-21 0.5805265263394475 2311 22-23 0.5731480301425691 2311 24-25 0.49512135479757546 2311 26-27 0.5617316997764519 2311 28-29 0.5327997323294724 2311 30-31 0.3454439517478889 2311 32-33 0.35408668543313127 2311 34-35 0.5833452890741739 2311 36-37 0.5165713786856116 2311 38-39 0.4855989634916611 2311 40-41 0.4257064343033221 2311 42-43 0.4293378846258733 2311 44-45 0.4759218775109062 2311 46-47 0.47304386514423413 2311 48-49 -6.41605614840951 2311 50-51 -1.539149495948152 2311 52-53 -0.038230542973010984 2311 54-55 0.4139751481018834 2311 56-57 0.4978480857217775 2311 58-59 0.4276703587973856 2311 60-61 0.39563490028826465 2311 62-63 0.2929241573479189 2311 64-65 0.3957280459849315 2311 66-67 0.4189131470949121 2311 68-69 0.4036418726385733 2311 70-71 0.32017760197309286 2311 72-73 -0.20155488574257419 2311 74-75 0.23939703676306223 2311 76-77 0.3453558274101418 2311 78-79 0.300421302028667 2311 80-81 0.3198095912695571 2311 82-83 0.31767466126099464 2311 84-85 0.3970828830816693 2311 86-87 0.45443243330941385 2311 88-89 0.43694450620702696 2311 90-91 0.4590152014494606 2311 92-93 0.4972054869222582 2311 94-95 0.42710540387173523 2311 96-97 0.4589752236983031 2311 98-99 0.48395203882088467 2311 100 0.7660809573456824 2312 1 0.07287514977407739 2312 2 0.1943760174274729 2312 3 0.14658217709333599 2312 4 -0.008942004280193316 2312 5 0.1481211879654225 2312 6 0.02985038350346514 2312 7 0.17792197000702004 2312 8 0.23688515701377355 2312 9 0.20640832564936318 2312 10-11 0.16786754468716936 2312 12-13 0.19785493070243376 2312 14-15 0.09828118962732191 2312 16-17 0.35364978539550407 2312 18-19 0.22449157126796848 2312 20-21 0.1816217148036401 2312 22-23 0.4135239794816812 2312 24-25 0.489190881298029 2312 26-27 0.4463092794953454 2312 28-29 0.5486509376852737 2312 30-31 0.5316287048586545 2312 32-33 0.5019473548085642 2312 34-35 0.09684532067519314 2312 36-37 0.3816419226224568 2312 38-39 0.4694499718742762 2312 40-41 0.19511314170621574 2312 42-43 0.24176995698173442 2312 44-45 0.32641684054159725 2312 46-47 0.38637517058414517 2312 48-49 -4.893154135987018 2312 50-51 -1.0521773540855648 2312 52-53 0.2184798746218135 2312 54-55 0.459666582210275 2312 56-57 0.659376582277261 2312 58-59 0.7461350748089401 2312 60-61 0.7489606261131954 2312 62-63 0.47687847003357575 2312 64-65 0.6702767039886552 2312 66-67 0.5770234509260774 2312 68-69 0.4519276435346882 2312 70-71 0.45089690119826287 2312 72-73 0.5456734821987155 2312 74-75 0.23639750903346268 2312 76-77 0.5217481914414357 2312 78-79 0.10068615925604973 2312 80-81 0.3322515704470099 2312 82-83 0.3513291132625369 2312 84-85 0.3698848773190413 2312 86-87 0.5362732002486261 2312 88-89 0.4440698083069279 2312 90-91 0.4068142973114348 2312 92-93 0.5081338162056213 2312 94-95 0.6344618199055105 2312 96-97 0.5499452915261038 2312 98-99 0.6632652673163939 2312 100 0.2838533299926169 2313 1 0.14567884256886998 2313 2 0.2508089977251018 2313 3 0.32573556550763527 2313 4 0.1288756544635561 2313 5 0.1396152237604369 2313 6 0.26563789667299176 2313 7 0.2450172374584767 2313 8 0.371775348448935 2313 9 0.5520750760356137 2313 10-11 0.27735251158532037 2313 12-13 0.1365826593900934 2313 14-15 0.20044381872673256 2313 16-17 0.24710560483084265 2313 18-19 0.4856780571873074 2313 20-21 0.3943391818621578 2313 22-23 0.5695388239426507 2313 24-25 0.3300727040364819 2313 26-27 0.4365613841730962 2313 28-29 0.43745094246925476 2313 30-31 0.5407870294666637 2313 32-33 0.383181609818422 2313 34-35 0.353415949017851 2313 36-37 -0.20652540129731278 2313 38-39 0.193894840352975 2313 40-41 0.4407423270059283 2313 42-43 0.4497054863776384 2313 44-45 0.48606234246225455 2313 46-47 0.3483944761030031 2313 48-49 -5.270972468836515 2313 50-51 -1.3219536597033752 2313 52-53 -0.09035392786737617 2313 54-55 0.0280612700395082 2313 56-57 0.02960198366918121 2313 58-59 0.20345697770074622 2313 60-61 0.43246308388101085 2313 62-63 0.3831564331904289 2313 64-65 0.5770272207124592 2313 66-67 0.5728672356167053 2313 68-69 0.5751150329731942 2313 70-71 0.4850785476906836 2313 72-73 0.3948870345511821 2313 74-75 0.5204266662816757 2313 76-77 0.5210826479219293 2313 78-79 0.48673768811701734 2313 80-81 -0.5132545361899936 2313 82-83 -0.0701610790424354 2313 84-85 0.3555084849373067 2313 86-87 0.46491190348661604 2313 88-89 0.5131905189013679 2313 90-91 0.6056246637531437 2313 92-93 0.5672974684235932 2313 94-95 0.42488954544008806 2313 96-97 0.5294672907497926 2313 98-99 0.6247579277182247 2313 100 0.7817282659797513 2314 1 0.052272061741156506 2314 2 0.21485655445158613 2314 3 0.22408058546323417 2314 4 0.0707534848687672 2314 5 0.18093969884893113 2314 6 0.2963141511489127 2314 7 0.05662081916376849 2314 8 0.20232252614390944 2314 9 0.3512188901054216 2314 10-11 0.25765067719555645 2314 12-13 0.16529202749698868 2314 14-15 0.1751089490990978 2314 16-17 0.4044216583608815 2314 18-19 0.49987049646225756 2314 20-21 0.5626903794395162 2314 22-23 0.2955602470053975 2314 24-25 0.3183850295098978 2314 26-27 0.38356208085449595 2314 28-29 0.4884915582496845 2314 30-31 0.3202125237969753 2314 32-33 0.37785855176849736 2314 34-35 0.10645642186678828 2314 36-37 0.25510678862794833 2314 38-39 0.14522662423880917 2314 40-41 -0.0360865055200108 2314 42-43 0.010465794215235746 2314 44-45 0.23933334714347865 2314 46-47 0.32875121731765233 2314 48-49 -6.392283203210617 2314 50-51 -1.744758554273865 2314 52-53 -1.139155035191827 2314 54-55 -0.7936681308364015 2314 56-57 -0.4800476894956063 2314 58-59 0.061648508527689216 2314 60-61 0.25328697430955316 2314 62-63 0.10173608000935275 2314 64-65 0.2910954060481217 2314 66-67 0.36211501699991544 2314 68-69 0.34731525280708553 2314 70-71 0.11000720251683305 2314 72-73 0.22200819704893604 2314 74-75 0.20327532594820852 2314 76-77 0.34014243055207416 2314 78-79 0.2087188413286185 2314 80-81 0.2050788826391674 2314 82-83 0.17499439841231634 2314 84-85 0.3107298029497869 2314 86-87 0.28858854861271865 2314 88-89 0.2927414509593653 2314 90-91 0.5591747794355761 2314 92-93 0.5202496832697925 2314 94-95 0.47309735554990695 2314 96-97 0.5111722354339925 2314 98-99 0.6450565229998197 2314 100 -0.2681705201109956 2315 1 0.09690938291121398 2315 2 0.16023147536736104 2315 3 0.25529638870992244 2315 4 0.11588040286411427 2315 5 0.2288321070668431 2315 6 0.2554730458015868 2315 7 0.3599703525831046 2315 8 0.25221532669750957 2315 9 0.4198241973765775 2315 10-11 0.1960162136669794 2315 12-13 0.42474248905437406 2315 14-15 0.19507775278272277 2315 16-17 0.30069368989323664 2315 18-19 0.41426826335337097 2315 20-21 0.37481576057025023 2315 22-23 0.29453636346239875 2315 24-25 0.33183837599037247 2315 26-27 0.4071728076411958 2315 28-29 0.3829112976712352 2315 30-31 0.40851135021095075 2315 32-33 0.4303858907668854 2315 34-35 0.4121004261677612 2315 36-37 0.3015962719963383 2315 38-39 0.43480365952347455 2315 40-41 0.4435135978903091 2315 42-43 0.5002275650435664 2315 44-45 0.413677754649477 2315 46-47 0.27453999608406576 2315 48-49 -5.021407588778729 2315 50-51 -1.002787210368247 2315 52-53 0.21300188002772558 2315 54-55 -0.022332106582609867 2315 56-57 0.3206292844270706 2315 58-59 0.48887712460904 2315 60-61 0.41601872193118794 2315 62-63 0.09098530280795103 2315 64-65 0.3388103105169833 2315 66-67 -0.38188655580714936 2315 68-69 0.28811534911554304 2315 70-71 0.2301699222259046 2315 72-73 0.35070672951428605 2315 74-75 0.5617560774659118 2315 76-77 0.48765640372168306 2315 78-79 0.3252758835159639 2315 80-81 0.1385239407035499 2315 82-83 0.15110384014808176 2315 84-85 0.1699290137747873 2315 86-87 0.23158788995645807 2315 88-89 0.19037015849909622 2315 90-91 0.17956229103567978 2315 92-93 0.334067044513521 2315 94-95 0.06553203230817672 2315 96-97 0.08595717615276399 2315 98-99 0.06313471039164043 2315 100 0.2667013233946989 2316 1 0.24938872902917097 2316 2 0.28278383821127306 2316 3 0.17937705845218943 2316 4 -0.032931649679760255 2316 5 0.17596750758720248 2316 6 0.20949218063873332 2316 7 0.3057891709812708 2316 8 0.3245852434083787 2316 9 0.5156292758624801 2316 10-11 0.4514917026585863 2316 12-13 0.30954346699932955 2316 14-15 0.3882490546604558 2316 16-17 0.444327058514034 2316 18-19 0.08856153814472378 2316 20-21 0.3692769302003711 2316 22-23 0.5436183918867954 2316 24-25 0.5316266512142178 2316 26-27 0.6236732558149285 2316 28-29 0.6079117378076972 2316 30-31 0.5017261299169107 2316 32-33 0.40685351624213695 2316 34-35 0.339169431145514 2316 36-37 0.26192084010764205 2316 38-39 0.3879608923434148 2316 40-41 0.028225706542023943 2316 42-43 -0.061616966509625115 2316 44-45 0.14179818823365054 2316 46-47 0.17004356195488413 2316 48-49 -5.124552995323214 2316 50-51 -1.1568495168517288 2316 52-53 0.11266760595053427 2316 54-55 0.291910020524206 2316 56-57 0.28636867924325315 2316 58-59 -0.008098181056169551 2316 60-61 0.11051885320666344 2316 62-63 0.15296685075642102 2316 64-65 0.2148177446132422 2316 66-67 0.1963639668070627 2316 68-69 -0.16409616439511865 2316 70-71 -0.32653458086767984 2316 72-73 -0.25466691572653843 2316 74-75 -0.0477207878132937 2316 76-77 0.04469655871095313 2316 78-79 0.06145272805134283 2316 80-81 -0.03589065866127683 2316 82-83 -0.022249838971767133 2316 84-85 0.07503061640734288 2316 86-87 0.1705861240524129 2316 88-89 0.04299601265088526 2316 90-91 -0.021064539836334717 2316 92-93 0.003509632730267498 2316 94-95 0.18979939645718602 2316 96-97 0.31285785763710194 2316 98-99 0.2970171043972094 2316 100 -0.4650221055668915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 0.0 11 7.0 12 21.0 13 159.0 14 615.0 15 1965.0 16 4938.0 17 11447.0 18 19423.0 19 25636.0 20 30256.0 21 37331.0 22 43400.0 23 40678.0 24 34977.0 25 32501.0 26 34461.0 27 38659.0 28 44692.0 29 51791.0 30 61205.0 31 75090.0 32 91862.0 33 119208.0 34 171571.0 35 237191.0 36 319416.0 37 541220.0 38 561251.0 39 88836.0 40 41.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.40233953057127 19.19329372818827 13.375029928205407 19.02933681303505 2 15.21550497500182 22.97269870777168 43.79409794403849 18.01769837318801 3 18.07009732532749 35.18032448148738 30.3967465817011 16.352831611484028 4 10.791674083270935 18.914400373061323 39.15529413149235 31.138631412175393 5 9.593689334666745 41.08002676628037 34.01075063239014 15.315533266662746 6 22.180459282849892 37.50818519704587 24.791339519215956 15.520016000888285 7 24.415105397395223 35.167356717229524 25.503511408170088 14.91402647720517 8 27.340664916499367 32.67157890092435 23.899453624243495 16.08830255833278 9 30.82407767618597 16.46213888964472 22.826682472924308 29.887100961245004 10-11 23.00666809323271 28.471224912843436 30.269507003777456 18.2525999901464 12-13 24.52887940305422 27.296426818440022 30.417100974504063 17.7575928040017 14-15 22.28356553078803 27.227427273036447 31.084518525395268 19.404488670780253 16-17 18.398102390468864 29.738995964105282 32.24846066482514 19.61444098060072 18-19 18.448336939231382 27.553546620870016 36.1665967179434 17.831519721955203 20-21 19.869952291451582 27.759786576308677 35.15687273700589 17.21338839523385 22-23 18.922526042863502 27.71803372474073 34.52965851337743 18.82978171901834 24-25 18.274308293274423 28.008655629164643 35.138409363609775 18.578626713951163 26-27 17.769890082092825 27.796902621028824 35.84384127348293 18.589366023395428 28-29 17.552296074768 28.341348244088366 36.12246957664395 17.983886104499682 30-31 19.58217323963729 27.72205549146762 35.857470482528505 16.83830078636658 32-33 18.531969689392756 28.147862019667773 35.905174188917684 17.414994102021787 34-35 17.963263930476646 28.790212540540978 35.86390182974288 17.382621699239497 36-37 18.640316723003256 28.84805448912492 34.8137509209897 17.69787786688212 38-39 18.201715151188633 29.34093056669788 34.48114368909938 17.976210593014102 40-41 17.95894708066066 28.817676437783334 35.39944262921349 17.82393385234252 42-43 18.283916299899623 28.810259182045144 34.1868986031599 18.718925914895337 44-45 17.698002008397616 28.963524160523257 34.75261446524299 18.585859365836136 46-47 17.441095319193845 28.443322015687077 34.80304004468469 19.312542620434385 48-49 17.72588225460899 29.415871005428755 34.14091762260499 18.717329117357266 50-51 17.614012455908615 29.280284420700227 34.06839202868919 19.037311094701973 52-53 17.132568892442052 30.23686537435055 33.00528678117161 19.62527895203579 54-55 17.415761426616033 29.881627051210984 33.07623504710742 19.626376475065566 56-57 18.075609271815388 29.53126149049139 32.497352590784075 19.895776646909148 58-59 17.482033434633692 29.385744778251492 33.60513451253569 19.527087274579124 60-61 18.720272587685756 30.322530473814872 32.59107949665258 18.366117441846782 62-63 17.668977750061774 30.311974196660746 32.98958173998988 19.029466313287603 64-65 17.48868138893603 29.652710831023153 32.75388982734372 20.104717952697097 66-67 17.979695076764084 29.73172566103172 31.750743367616202 20.53783589458799 68-69 17.411289756087513 29.947621392626612 32.27284613112894 20.368242720156935 70-71 17.455149731363615 29.883053345165667 32.709555320265544 19.952241603205177 72-73 17.92700419621155 30.141765792151 31.531768912509882 20.39946109912757 74-75 18.07862519619345 30.464482758050117 31.045731987019288 20.411160058737153 76-77 17.348422808809076 29.71705762582724 32.39146686631686 20.54305269904682 78-79 17.320713132186697 30.051702304155597 31.626653547128853 21.000931016528853 80-81 16.97622011796551 30.81587909013162 31.060531411019575 21.147369380883294 82-83 17.534245970867573 30.74029103007821 30.465584141464213 21.259878857590007 84-85 17.59168085755438 31.160890924800892 29.53280490089915 21.71462331674558 86-87 16.769681526132626 31.222549239901486 30.429223881991195 21.57854535197469 88-89 17.358123331614532 30.065161279407537 31.023124401882203 21.553590987095735 90-91 18.244883316879754 30.233833278202045 30.59433490612266 20.92694849879554 92-93 17.24721145266645 30.99008391887909 30.505118365829535 21.257586262624926 94-95 17.0763127313147 30.221946287465144 30.301019122168356 22.4007218590518 96-97 17.742319770654802 29.80505878680449 30.37053501010941 22.0820864324313 98-99 17.440519113937874 30.158917232936787 29.976266849746064 22.42429680337927 100 16.18118013740525 31.002725815878147 30.694172727202513 22.121921319514097 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7972.0 1 5417.0 2 11422.0 3 16041.5 4 8642.0 5 5414.5 6 7237.0 7 11415.0 8 15226.5 9 16148.0 10 13801.5 11 11277.0 12 10995.0 13 11456.5 14 11912.0 15 12079.5 16 12292.0 17 12690.5 18 13025.0 19 13345.5 20 13837.0 21 14479.5 22 15313.0 23 16425.5 24 17271.5 25 19627.0 26 24998.5 27 29975.0 28 31433.0 29 37335.0 30 51041.0 31 58433.5 32 66217.0 33 81057.0 34 99782.5 35 113123.0 36 123904.5 37 135638.0 38 129318.0 39 113627.0 40 96648.5 41 82488.5 42 74191.5 43 69912.5 44 69117.0 45 68834.5 46 66788.5 47 64066.0 48 65663.5 49 75084.5 50 99476.0 51 101769.5 52 76495.5 53 60126.0 54 50168.0 55 41351.0 56 34472.5 57 30004.5 58 25016.0 59 17946.5 60 11156.5 61 7147.0 62 4844.0 63 3230.0 64 2118.5 65 1366.5 66 890.5 67 665.5 68 510.0 69 367.0 70 270.0 71 233.5 72 203.0 73 164.0 74 134.0 75 91.0 76 54.0 77 40.0 78 33.5 79 18.5 80 8.5 81 4.0 82 6.0 83 6.5 84 2.0 85 0.5 86 0.5 87 1.0 88 1.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030994356306413954 2 0.0019118701398974209 3 1.1030020037869734E-4 4 0.026692648491644762 5 3.6766733459565784E-4 6 3.676673345956578E-5 7 3.676673345956578E-5 8 0.0040443406805522365 9 0.009265216831810578 10-11 0.001176535470706105 12-13 1.8383366729782892E-4 14-15 9.191683364891446E-5 16-17 2.573671342169605E-4 18-19 1.838336672978289E-5 20-21 7.353346691913156E-5 22-23 2.573671342169605E-4 24-25 5.515010018934867E-5 26-27 7.353346691913156E-5 28-29 8.64018236299796E-4 30-31 0.0022979208412228615 32-33 0.010074084967921025 34-35 0.019615052300678348 36-37 0.023107891979337096 38-39 0.022593157710903176 40-41 0.004283324448039414 42-43 0.00514734268433921 44-45 0.009890251300623196 46-47 0.012886740077577808 48-49 1.2455282460429804 50-51 0.01830983326286376 52-53 0.019137084765703992 54-55 0.009835101200433847 56-57 0.007721014026508814 58-59 0.007904847693806644 60-61 0.005073809217420078 62-63 0.007426880158832289 64-65 0.020056253102193135 66-67 0.029597220434950458 68-69 0.029450153501112197 70-71 0.027464749894295645 72-73 0.02248285751052448 74-75 0.01016600180156994 76-77 0.01569939518723459 78-79 0.00889754949721492 80-81 0.0026472048090887363 82-83 0.014927293784583708 84-85 0.024835928451936686 86-87 0.024376344283692116 88-89 0.033237127047447475 90-91 0.02145338897365664 92-93 0.01391620861444565 94-95 0.007555563725940768 96-97 0.00393404048017354 98-99 0.016434729856425905 100 0.037906502196812325 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2719850.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.73361887648424 #Duplication Level Percentage of deduplicated Percentage of total 1 84.4635236386403 44.54067264524975 2 10.404764403022662 10.973617610572143 3 2.3208082026109236 3.6715384572570864 4 0.878131153894594 1.8522813437217938 5 0.4392842931082333 1.1582525245597683 6 0.26467893427278777 0.8374486826745126 7 0.18077761037692158 0.6673140324912701 8 0.13351285646973143 0.5632492870548449 9 0.10028248205711655 0.4759432369909067 >10 0.6188107610581849 6.388298657876243 >50 0.08546020086607262 3.189271120634771 >100 0.09207043299464882 10.061588473430625 >500 0.011592867667707831 4.3025185088592055 >1k 0.005524118150384256 4.942695844421398 >5k 2.334134429739826E-4 0.6566710411890666 >10k+ 5.446313669392928E-4 5.718638533016689 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32372 1.1902126955530636 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28505 1.0480357372649227 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24468 0.8996084342886556 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18431 0.677647664393257 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 12534 0.46083423718219757 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12007 0.44145816864900633 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11063 0.4067503722631763 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5496 0.20206996709377353 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 5362 0.19714322481019175 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5147 0.1892383771163851 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 4156 0.15280254425795542 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 3699 0.13600014706693384 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 3429 0.1260731290328511 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3403 0.12511719396290236 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 3139 0.115410776329577 No Hit GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2917 0.10724856150155339 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 2791 0.1026159530856481 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.676673345956579E-5 0.0 10-11 0.0 0.0 0.0 3.676673345956579E-5 0.0 12-13 0.0 0.0 0.0 7.353346691913158E-5 0.0 14-15 0.0 0.0 0.0 7.353346691913158E-5 0.0 16-17 0.0 0.0 0.0 9.191683364891446E-5 0.0 18-19 0.0 0.0 0.0 2.389837674871776E-4 0.0 20-21 0.0 0.0 0.0 8.272515028402302E-4 0.0 22-23 0.0 0.0 0.0 0.004062724047282019 0.0 24-25 0.0 0.0 0.0 0.011930805007629098 0.0 26-27 0.0 0.0 0.0 0.022795374744930787 0.0 28-29 0.0 0.0 0.0 0.039965439270548006 0.0 30-31 0.0 0.0 0.0 0.08096034707796386 0.0 32-33 0.0 0.0 0.0 0.16510101660018017 0.0 34-35 0.0 0.0 0.0 0.28201922900159937 0.0 36-37 0.0 0.0 0.0 0.4421751199514679 0.0 38-39 0.0 0.0 0.0 0.6971891832270162 0.0 40-41 0.0 0.0 0.0 1.0408662242403075 0.0 42-43 0.0 0.0 0.0 1.355203411952865 0.0 44-45 0.0 0.0 0.0 1.6920969906428662 0.0 46-47 0.0 0.0 0.0 2.063937349486185 0.0 48-49 0.0 0.0 0.0 2.457837748405243 0.0 50-51 0.0 0.0 0.0 2.9073294483151644 0.0 52-53 0.0 0.0 0.0 3.3925400297810544 0.0 54-55 0.0 0.0 0.0 3.9250693972094046 0.0 56-57 0.0 0.0 0.0 4.472084857620825 0.0 58-59 0.0 0.0 0.0 5.070518594775447 0.0 60-61 0.0 0.0 0.0 5.656451642553817 0.0 62-63 0.0 0.0 0.0 6.289207125392945 0.0 64-65 0.0 0.0 0.0 6.907219148114786 0.0 66-67 3.676673345956579E-5 0.0 0.0 7.562218504696951 0.0 68-69 3.676673345956579E-5 0.0 0.0 8.291045462065922 0.0 70-71 3.676673345956579E-5 0.0 0.0 9.09410445428976 0.0 72-73 3.676673345956579E-5 0.0 0.0 9.80451127819549 0.0 74-75 3.676673345956579E-5 0.0 0.0 10.508741290880012 0.0 76-77 3.676673345956579E-5 0.0 0.0 11.25392944463849 0.0 78-79 3.676673345956579E-5 0.0 0.0 12.053752964317884 0.0 80-81 3.676673345956579E-5 0.0 0.0 12.810927073184184 0.0 82-83 3.676673345956579E-5 0.0 0.0 13.589609721124326 0.0 84-85 3.676673345956579E-5 0.0 0.0 14.36577384782249 0.0 86-87 3.676673345956579E-5 0.0 0.0 15.144787396363771 0.0 88 3.676673345956579E-5 0.0 0.0 15.759913230509035 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4360 0.0 70.056526 2 ACCTGGG 4410 0.0 66.82256 3 TATAACG 620 0.0 59.02786 2 TAACGCA 660 0.0 58.300457 4 CCTGGGG 2030 0.0 57.557953 4 TACGTGG 645 0.0 53.83022 2 GTACGTG 660 0.0 53.332417 1 ACGTGGG 635 0.0 52.467052 3 TATCACG 385 0.0 51.184998 2 GTACACG 335 0.0 49.033985 1 CTGGGGG 1340 0.0 44.808815 5 CGTGGGA 420 0.0 43.572987 4 ATCACGC 450 0.0 42.753666 3 TACTTGG 980 0.0 42.131866 2 ACACGGG 385 0.0 41.440044 3 GTACTTG 1015 0.0 41.15266 1 ACAACGC 230 0.0 40.804134 3 GTACCCG 210 0.0 40.22788 1 TCACGCA 480 0.0 40.081566 4 GTATCAC 540 0.0 39.97956 1 >>END_MODULE