FastQCFastQC Report
Mon 6 Jun 2016
ERR1211357_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211357_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9197179
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337060.36648193973391185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248510.2702024175021493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157560.17131339946738017No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173800.046.5537871
GTATCAA304550.040.2524341
TACCTGG67850.036.604892
ATCAACG370150.032.4870453
CAACGCA370850.032.3989755
TATCAAC382250.031.6749612
AACGCAG397600.030.6220346
TCAACGC393300.030.5744
GTACATG451950.029.1852861
TACATGG470150.027.7939872
ACCTGGG87900.027.7457313
ACGCAGA445950.026.9656757
ACATGGG474250.026.6540153
GTACTAG39150.026.554421
CGCAGAG457450.026.2449728
GTACCTG96750.026.1095661
CATGGGG293500.024.7518274
GCAGAGT495700.023.7185699
ATGGGGG203300.022.674125
TCTAACG18500.022.6294142
Produced by FastQC (version 0.11.5)