##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211357_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9197179 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.441961388377894 31.0 31.0 34.0 28.0 34.0 2 31.44695291893308 31.0 31.0 34.0 28.0 34.0 3 31.499267655875787 31.0 31.0 34.0 28.0 34.0 4 35.11870879103255 37.0 35.0 37.0 32.0 37.0 5 35.167292601350916 37.0 35.0 37.0 32.0 37.0 6 35.23168995623549 37.0 35.0 37.0 32.0 37.0 7 35.16643266375483 37.0 35.0 37.0 32.0 37.0 8 35.16721768707557 37.0 35.0 37.0 32.0 37.0 9 36.72938615199291 39.0 37.0 39.0 32.0 39.0 10-11 36.65969690271332 39.0 37.0 39.0 32.0 39.0 12-13 36.57256545729946 39.0 37.0 39.0 32.0 39.0 14-15 37.70381200583353 40.0 37.0 41.0 32.0 41.0 16-17 37.70579658175621 40.0 37.0 41.0 32.0 41.0 18-19 37.70921339032327 40.0 37.0 41.0 32.0 41.0 20-21 37.67611139241717 40.0 37.0 41.0 32.0 41.0 22-23 37.55217616184267 40.0 37.0 41.0 32.0 41.0 24-25 37.440911066317184 40.0 37.0 41.0 31.0 41.0 26-27 37.29336522644606 40.0 36.5 41.0 31.0 41.0 28-29 37.15758875629147 40.0 36.0 41.0 31.0 41.0 30-31 36.96578032242277 39.0 36.0 41.0 30.0 41.0 32-33 36.837957649840234 39.0 36.0 41.0 30.0 41.0 34-35 36.6959875413972 39.0 35.0 41.0 30.0 41.0 36-37 36.59983604755328 39.0 35.0 41.0 30.0 41.0 38-39 36.44859070373644 39.0 35.0 41.0 29.5 41.0 40-41 36.27402956928424 39.0 35.0 40.0 29.0 41.0 42-43 36.1182538145664 39.0 35.0 40.0 28.0 41.0 44-45 35.97017036419537 39.0 35.0 40.0 27.5 41.0 46-47 35.791763213481005 38.5 35.0 40.0 27.0 41.0 48-49 35.547769321440846 38.0 34.0 40.0 26.0 41.0 50-51 34.86567631226923 37.5 33.5 39.5 25.0 40.5 52-53 35.0953080286901 38.0 34.0 40.0 26.0 40.5 54-55 35.45190400230331 38.0 34.0 40.0 26.0 41.0 56-57 35.34893889745975 38.0 34.0 40.0 26.0 41.0 58-59 35.125098032777224 38.0 34.0 40.0 26.0 41.0 60-61 34.855719128658905 37.5 34.0 40.0 26.0 41.0 62-63 34.5730762661029 37.0 33.0 40.0 25.5 41.0 64-65 34.29883761096745 36.5 33.0 40.0 25.0 41.0 66-67 34.08276554147744 36.0 33.0 39.0 25.0 41.0 68-69 33.74640577290059 35.5 33.0 39.0 24.0 41.0 70-71 33.4136823910897 35.0 32.0 39.0 24.0 40.5 72-73 33.05283560317788 35.0 32.0 38.0 23.0 40.0 74-75 32.679170156414266 35.0 32.0 37.0 22.5 39.5 76-77 32.33139166911941 35.0 31.0 36.5 22.0 39.0 78-79 31.96799002172297 35.0 31.0 36.0 20.5 39.0 80-81 31.637890542306508 35.0 31.0 36.0 20.0 37.5 82-83 31.316271054417882 34.5 30.5 35.0 20.0 37.0 84-85 31.010929383890428 34.0 30.0 35.0 19.5 37.0 86-87 30.75449760192772 34.0 30.0 35.0 19.0 36.0 88-89 30.49661749542985 34.0 29.5 35.0 18.0 36.0 90-91 30.232574031667752 34.0 29.0 35.0 18.0 36.0 92-93 29.985024484138016 34.0 29.0 35.0 17.0 35.0 94-95 29.756756664190185 34.0 29.0 35.0 15.5 35.0 96-97 29.545446707082682 34.0 29.0 35.0 15.0 35.0 98-99 29.303243364079357 34.0 27.0 35.0 12.0 35.0 100 27.596088865944655 32.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.32237224493891503 1101 2 -0.3211030892461153 1101 3 -0.36251964343216514 1101 4 -0.2529792719983135 1101 5 -0.30813987927871267 1101 6 -0.2489600716525544 1101 7 -0.2928123728502712 1101 8 -0.4076315556269776 1101 9 -0.4710922239485811 1101 10-11 -0.41447289120552 1101 12-13 -0.3858219295679035 1101 14-15 -0.46532352164305735 1101 16-17 -0.44921394392903125 1101 18-19 -0.5496789801669166 1101 20-21 -0.6149199189836807 1101 22-23 -0.5546817074309658 1101 24-25 -0.61356893754499 1101 26-27 -0.6441754811929883 1101 28-29 -0.6721999487453516 1101 30-31 -0.736886885254016 1101 32-33 -0.6680578543617699 1101 34-35 -0.7079040659146685 1101 36-37 -0.8259055611361319 1101 38-39 -0.8030702747173422 1101 40-41 -0.8787157575697222 1101 42-43 -0.8894642746983692 1101 44-45 -1.009788563430611 1101 46-47 -0.8642656813919061 1101 48-49 -0.8402274012389128 1101 50-51 -0.8350611573514612 1101 52-53 -0.7040869547168427 1101 54-55 -0.6662121707337363 1101 56-57 -0.6463931372244147 1101 58-59 -0.678519980794789 1101 60-61 -0.6175280153161893 1101 62-63 -0.6791555539623175 1101 64-65 -0.6927028578806613 1101 66-67 -0.8384578348883096 1101 68-69 -0.768967905064315 1101 70-71 -0.7431363080250364 1101 72-73 -0.803888651250972 1101 74-75 -0.8807219572531473 1101 76-77 -0.8445489333311862 1101 78-79 -0.9007500887288629 1101 80-81 -0.9451391099780899 1101 82-83 -0.9709557673068367 1101 84-85 -0.9828883030331639 1101 86-87 -0.9980070969216328 1101 88-89 -1.1529874239704938 1101 90-91 -1.088499061405706 1101 92-93 -1.178659874019047 1101 94-95 -1.1961002515229922 1101 96-97 -1.392587679792591 1101 98-99 -1.4449151082980443 1101 100 -1.4548855588075718 1102 1 -0.4213986473200144 1102 2 -0.39004510860165453 1102 3 -0.40388096961680375 1102 4 -0.3244460012288215 1102 5 -0.3142153945948607 1102 6 -0.3063028507954826 1102 7 -0.3510526562454004 1102 8 -0.34092857037470736 1102 9 -0.4100608778432999 1102 10-11 -0.4010748166066378 1102 12-13 -0.4487103096128422 1102 14-15 -0.571045075894915 1102 16-17 -0.5959446199318421 1102 18-19 -0.5885315773489737 1102 20-21 -0.6811402699808013 1102 22-23 -0.699820384856352 1102 24-25 -0.6627931283430613 1102 26-27 -0.7089521423607508 1102 28-29 -0.8383352296957085 1102 30-31 -0.7118344970961203 1102 32-33 -0.7143115158386664 1102 34-35 -0.8145851126372392 1102 36-37 -0.8838163944184174 1102 38-39 -1.036112444048939 1102 40-41 -0.9831535125928497 1102 42-43 -0.9536566477805124 1102 44-45 -0.945185056913104 1102 46-47 -0.9656034225778427 1102 48-49 -1.0539959909124121 1102 50-51 -0.9536915050355503 1102 52-53 -0.9121535214887899 1102 54-55 -0.8112415797816226 1102 56-57 -0.833508423029329 1102 58-59 -0.8753914589272256 1102 60-61 -0.8506017146782412 1102 62-63 -0.8889237747433967 1102 64-65 -0.8796501770502445 1102 66-67 -0.7829564958668769 1102 68-69 -0.8660448419858682 1102 70-71 -0.9078169499782476 1102 72-73 -0.9494919872413305 1102 74-75 -1.0154883190575674 1102 76-77 -0.9475073597653214 1102 78-79 -0.9796992752860838 1102 80-81 -1.0229555898675393 1102 82-83 -1.129440862482344 1102 84-85 -1.1573980672568638 1102 86-87 -1.072999138119684 1102 88-89 -1.1634545753133736 1102 90-91 -1.3134961156999587 1102 92-93 -1.192277051067535 1102 94-95 -1.187721011949023 1102 96-97 -1.229245314946521 1102 98-99 -1.3287754246061745 1102 100 -1.337143567498579 1103 1 -0.3981679253174768 1103 2 -0.48118810666126777 1103 3 -0.47598177539569875 1103 4 -0.3987241052418966 1103 5 -0.3845073207368159 1103 6 -0.45131655754157407 1103 7 -0.47662810631988606 1103 8 -0.40287876225379193 1103 9 -0.5353341440344721 1103 10-11 -0.596832953541238 1103 12-13 -0.625121382597932 1103 14-15 -0.7063190159523032 1103 16-17 -0.6696395704852307 1103 18-19 -0.7340291814761883 1103 20-21 -0.8364545306602906 1103 22-23 -0.8333324815229375 1103 24-25 -0.8400340550991814 1103 26-27 -0.8767281284948538 1103 28-29 -0.8697980627099398 1103 30-31 -0.8105536905147304 1103 32-33 -0.8614006729472123 1103 34-35 -1.0846801968887618 1103 36-37 -1.0244722409566194 1103 38-39 -1.0577570846334652 1103 40-41 -1.0552925233226773 1103 42-43 -1.0967572272094657 1103 44-45 -1.139137769934976 1103 46-47 -1.1291137011495351 1103 48-49 -1.0684859384986751 1103 50-51 -1.0388197191230404 1103 52-53 -0.9330391506484972 1103 54-55 -0.9147241050482435 1103 56-57 -0.9108515624280571 1103 58-59 -0.9877217900681643 1103 60-61 -0.8634597925007697 1103 62-63 -0.959931035795897 1103 64-65 -0.9557364887538782 1103 66-67 -0.9894505119924304 1103 68-69 -0.978093819581062 1103 70-71 -1.0106504710405915 1103 72-73 -1.1761850953177628 1103 74-75 -1.0135752877327704 1103 76-77 -1.0131250349499012 1103 78-79 -1.0596380475325446 1103 80-81 -1.038971047705875 1103 82-83 -1.1526190766754283 1103 84-85 -1.1881718672614383 1103 86-87 -1.2284335812076463 1103 88-89 -1.31137176233851 1103 90-91 -1.2417037333064123 1103 92-93 -1.2441746372249796 1103 94-95 -1.5415045211947174 1103 96-97 -1.4492962331228405 1103 98-99 -1.550538942103369 1103 100 -1.5436532303754014 1104 1 -0.43482525197293853 1104 2 -0.48670379603801095 1104 3 -0.46621047945379246 1104 4 -0.2878994484310198 1104 5 -0.24047523994742903 1104 6 -0.2529261232412878 1104 7 -0.3404662983011164 1104 8 -0.33795640578738784 1104 9 -0.4715204107385276 1104 10-11 -0.40223898818456405 1104 12-13 -0.49753270195468247 1104 14-15 -0.6373340616255732 1104 16-17 -0.6316108688832358 1104 18-19 -0.6016768904444376 1104 20-21 -0.6304186081175658 1104 22-23 -0.6030203927921534 1104 24-25 -0.6941784718669908 1104 26-27 -0.6531262135537688 1104 28-29 -0.665234010632382 1104 30-31 -0.686163366739045 1104 32-33 -0.7788303516685673 1104 34-35 -0.7472681816363647 1104 36-37 -0.7548317119940506 1104 38-39 -0.7746745125212797 1104 40-41 -0.7614418359210546 1104 42-43 -0.7827115457536564 1104 44-45 -0.8643263781232093 1104 46-47 -0.8247163570148075 1104 48-49 -0.8221206117040367 1104 50-51 -0.7700703459408089 1104 52-53 -0.6534777593484264 1104 54-55 -0.6984370767478083 1104 56-57 -0.6649722880425486 1104 58-59 -0.7123440108349115 1104 60-61 -0.6201772322597421 1104 62-63 -0.7004109274817907 1104 64-65 -0.7227475555414387 1104 66-67 -0.7067500172924994 1104 68-69 -0.645371578142111 1104 70-71 -0.7759602776430015 1104 72-73 -0.7598417813398797 1104 74-75 -0.7676682286901197 1104 76-77 -0.7989770670675327 1104 78-79 -0.8057152428274748 1104 80-81 -0.7983656901280867 1104 82-83 -0.8897831279342832 1104 84-85 -0.8205110079121809 1104 86-87 -0.9424541899525991 1104 88-89 -0.8765137864533372 1104 90-91 -0.8313194351683428 1104 92-93 -0.9009657088838097 1104 94-95 -1.0458987952015804 1104 96-97 -1.005316344507083 1104 98-99 -1.0409258089150875 1104 100 -1.0966656398322137 1105 1 -0.3443969358483372 1105 2 -0.3600993738002174 1105 3 -0.32572037743712556 1105 4 -0.21643345573574635 1105 5 -0.18219534752482502 1105 6 -0.26068007782982505 1105 7 -0.20443381524269455 1105 8 -0.24996810780218937 1105 9 -0.3971525795049402 1105 10-11 -0.38690818884998635 1105 12-13 -0.42312060054654665 1105 14-15 -0.48773179373360165 1105 16-17 -0.6268504116210067 1105 18-19 -0.5691548661054568 1105 20-21 -0.5862174180979736 1105 22-23 -0.6203260965626072 1105 24-25 -0.5296689002996899 1105 26-27 -0.5971278526285673 1105 28-29 -0.6696947480295208 1105 30-31 -0.665876901696457 1105 32-33 -0.7034165902017477 1105 34-35 -0.7011722048305487 1105 36-37 -0.7228107945551656 1105 38-39 -0.7093813093191343 1105 40-41 -0.7508417375299388 1105 42-43 -0.8133841609639489 1105 44-45 -0.854291474923059 1105 46-47 -0.7931535003615693 1105 48-49 -0.8659582267219719 1105 50-51 -0.7682255998983152 1105 52-53 -0.6192210378011183 1105 54-55 -0.6389533794800286 1105 56-57 -0.693679907875115 1105 58-59 -0.7101078569950374 1105 60-61 -0.6811161912956933 1105 62-63 -0.7320134495119603 1105 64-65 -0.6729006213457467 1105 66-67 -0.6674451260648127 1105 68-69 -0.6003868147762574 1105 70-71 -0.6458277352468045 1105 72-73 -0.7407435580922979 1105 74-75 -0.6702096980985246 1105 76-77 -0.6378113946096988 1105 78-79 -0.6571365223550103 1105 80-81 -0.6918547103087569 1105 82-83 -0.7942420292412287 1105 84-85 -0.7284842732732599 1105 86-87 -0.7481083859724222 1105 88-89 -0.8010949538802841 1105 90-91 -0.7891617552402472 1105 92-93 -0.8849881686384649 1105 94-95 -0.8805121845541208 1105 96-97 -1.0296828642476719 1105 98-99 -1.0669541068536539 1105 100 -1.068432401586982 1106 1 -0.5300775256450727 1106 2 -0.6158015115718136 1106 3 -0.5413875035150788 1106 4 -0.393500677642983 1106 5 -0.4233035782475838 1106 6 -0.3909671045481886 1106 7 -0.5559887701964996 1106 8 -0.5328027295282354 1106 9 -0.6013226681977173 1106 10-11 -0.6293795189959965 1106 12-13 -0.5976848530610468 1106 14-15 -0.7623662034557555 1106 16-17 -0.8606458229960907 1106 18-19 -0.7754166583007418 1106 20-21 -0.7981157920183648 1106 22-23 -0.7804297627365386 1106 24-25 -0.7708601749571855 1106 26-27 -0.7285685151447936 1106 28-29 -0.8339074307008261 1106 30-31 -0.9122629698497207 1106 32-33 -0.892707005993195 1106 34-35 -0.8516711462303803 1106 36-37 -0.8951050974515837 1106 38-39 -0.8843315119237403 1106 40-41 -0.9350399626780046 1106 42-43 -0.8261635783147199 1106 44-45 -0.8281973804967464 1106 46-47 -0.9756547848342905 1106 48-49 -0.9613521226133201 1106 50-51 -0.9161715630622567 1106 52-53 -0.794561245658322 1106 54-55 -0.9078452446329024 1106 56-57 -0.7473785071698558 1106 58-59 -0.7658850601691682 1106 60-61 -0.8157973051932146 1106 62-63 -0.7981722814212517 1106 64-65 -0.8269379237427472 1106 66-67 -0.9277691063203406 1106 68-69 -0.8289688585630941 1106 70-71 -0.812366097055012 1106 72-73 -0.7674231745341729 1106 74-75 -0.903014809827809 1106 76-77 -0.8138903227282945 1106 78-79 -0.8577459538982772 1106 80-81 -0.9171188129039045 1106 82-83 -0.7671531600076484 1106 84-85 -0.8242185348834958 1106 86-87 -0.8869996419819586 1106 88-89 -0.9250725170717402 1106 90-91 -0.9165364511537497 1106 92-93 -0.997617707183565 1106 94-95 -1.002082291912231 1106 96-97 -0.9601723319189652 1106 98-99 -1.0605694490279731 1106 100 -1.0915703622121633 1107 1 -0.5320836432991278 1107 2 -0.5059893127811534 1107 3 -0.5830777929312738 1107 4 -0.4048073992372778 1107 5 -0.3075386849769757 1107 6 -0.38961598704979394 1107 7 -0.4266295700576137 1107 8 -0.42563567567005123 1107 9 -0.5812733696440233 1107 10-11 -0.5455425772508633 1107 12-13 -0.5224816230900444 1107 14-15 -0.6981183338505303 1107 16-17 -0.7709272022673659 1107 18-19 -0.7465347162344642 1107 20-21 -0.7057305842770063 1107 22-23 -0.7389378651503051 1107 24-25 -0.799174182371182 1107 26-27 -0.8028469730202872 1107 28-29 -0.7915236814135795 1107 30-31 -0.8018665900693875 1107 32-33 -0.802480289301549 1107 34-35 -0.7343968721548819 1107 36-37 -0.8274192939036666 1107 38-39 -0.8175797711044979 1107 40-41 -0.8422572069906664 1107 42-43 -0.9439610307319555 1107 44-45 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0.6040501050060527 2311 12-13 0.6783376914154005 2311 14-15 0.831258189869061 2311 16-17 0.8038513646644816 2311 18-19 0.7277366936208836 2311 20-21 0.7789568086220697 2311 22-23 0.8558031602476532 2311 24-25 0.8736498305988221 2311 26-27 0.925215536246597 2311 28-29 0.8832117413145468 2311 30-31 0.9045264086593718 2311 32-33 0.9916818745686413 2311 34-35 0.9780261670929704 2311 36-37 0.9218806761992226 2311 38-39 0.9209801482948308 2311 40-41 0.9724995690799858 2311 42-43 1.0366799219892897 2311 44-45 1.1214322928729956 2311 46-47 1.0339555228806319 2311 48-49 1.0133625090351757 2311 50-51 1.0113802213071068 2311 52-53 0.9463213621041646 2311 54-55 0.9065544800067116 2311 56-57 0.8872351667567742 2311 58-59 0.8341087817022554 2311 60-61 0.829022306293993 2311 62-63 0.8421641177871848 2311 64-65 0.8936077580797388 2311 66-67 0.9057568714639288 2311 68-69 0.93560822394371 2311 70-71 0.8556944217340785 2311 72-73 0.8473201152813772 2311 74-75 0.8397976167350194 2311 76-77 0.8362806728419514 2311 78-79 0.8432005512633793 2311 80-81 0.8839858782589936 2311 82-83 0.8835271786093521 2311 84-85 0.9606195434614762 2311 86-87 0.9517735141755139 2311 88-89 0.959307626452695 2311 90-91 1.0425767972643278 2311 92-93 0.963824149902706 2311 94-95 1.0374644764395597 2311 96-97 1.0931173571370643 2311 98-99 1.1571884392022547 2311 100 1.1690503650586024 2312 1 0.5330798696585042 2312 2 0.5575390706001713 2312 3 0.5396700474636269 2312 4 0.42660114320067777 2312 5 0.44540763341052525 2312 6 0.4270523935891717 2312 7 0.4760767922075786 2312 8 0.5059819713887777 2312 9 0.6627551508550837 2312 10-11 0.65294484219244 2312 12-13 0.6822540823809575 2312 14-15 0.8335781559341982 2312 16-17 0.7969829513045568 2312 18-19 0.7459992204171186 2312 20-21 0.7856249964168143 2312 22-23 0.8044599135415922 2312 24-25 0.8574108992139102 2312 26-27 0.8563759119378886 2312 28-29 0.8641572797820842 2312 30-31 0.9101157902953432 2312 32-33 0.8869213629677475 2312 34-35 0.9024629591902382 2312 36-37 0.8788015685671908 2312 38-39 0.9492377251247532 2312 40-41 0.9634828862494302 2312 42-43 0.9852459142838086 2312 44-45 0.9293920088404377 2312 46-47 0.9486199070866661 2312 48-49 0.9605111633006231 2312 50-51 1.0107157755482064 2312 52-53 0.8732162657192291 2312 54-55 0.8461625721078221 2312 56-57 0.7828367116981596 2312 58-59 0.7929096193003176 2312 60-61 0.8036941252793639 2312 62-63 0.7931430488675488 2312 64-65 0.7972242369565237 2312 66-67 0.8196407280538835 2312 68-69 0.8329075040253002 2312 70-71 0.8121050364878641 2312 72-73 0.8633799910718238 2312 74-75 0.8281546734824801 2312 76-77 0.8710487529084361 2312 78-79 0.8962204793101449 2312 80-81 0.9370605631633246 2312 82-83 0.948259292423689 2312 84-85 0.9569534165799105 2312 86-87 1.0571321243645215 2312 88-89 0.9966578844314427 2312 90-91 1.0656797230347053 2312 92-93 1.146484363061525 2312 94-95 1.1594239271076816 2312 96-97 1.1197225726243083 2312 98-99 1.1899636198681627 2312 100 1.2546866677140116 2313 1 0.5011260151452923 2313 2 0.47790612646320696 2313 3 0.555000660254894 2313 4 0.40939021190380487 2313 5 0.4273857259095095 2313 6 0.4596374355234545 2313 7 0.47086137268282613 2313 8 0.46064890147256676 2313 9 0.6274184448938556 2313 10-11 0.6297326921921496 2313 12-13 0.6620495411363905 2313 14-15 0.7911080982823648 2313 16-17 0.7882929720835392 2313 18-19 0.739415007337243 2313 20-21 0.746696012326538 2313 22-23 0.7346426419875556 2313 24-25 0.7248451206114837 2313 26-27 0.7616106815819066 2313 28-29 0.7832549570595901 2313 30-31 0.8278743987030808 2313 32-33 0.9666712704870903 2313 34-35 0.8854669395382402 2313 36-37 0.890104942125447 2313 38-39 0.951947609696127 2313 40-41 0.9760906484313949 2313 42-43 0.775989501458497 2313 44-45 0.9286343201014375 2313 46-47 0.9672828957726409 2313 48-49 1.0061377655696475 2313 50-51 0.8882676723320202 2313 52-53 0.8018554368176112 2313 54-55 0.7752188434627314 2313 56-57 0.8499966397743108 2313 58-59 0.7755665715405868 2313 60-61 0.8273561638168587 2313 62-63 0.8873685917609393 2313 64-65 0.8112929695938362 2313 66-67 0.8330968197380386 2313 68-69 0.8546501784048033 2313 70-71 0.8095601608049137 2313 72-73 0.8393404533695659 2313 74-75 0.8703830021820593 2313 76-77 0.9276278906610145 2313 78-79 0.9424530832960301 2313 80-81 0.9554917166609407 2313 82-83 0.9355438444326687 2313 84-85 0.9239559522300951 2313 86-87 0.9587401352444687 2313 88-89 0.9813206324038788 2313 90-91 1.0032416198101863 2313 92-93 1.0506069261059778 2313 94-95 1.1063735824992094 2313 96-97 1.0946955591773992 2313 98-99 1.1312943463856406 2313 100 1.147790676097813 2314 1 0.360159236770361 2314 2 0.26162228037748037 2314 3 0.3490671079930543 2314 4 0.30130470796429876 2314 5 0.2724002432354311 2314 6 0.28526970645837224 2314 7 0.3892983468535931 2314 8 0.3800740748324287 2314 9 0.48584551922262165 2314 10-11 0.5253595525031756 2314 12-13 0.5250147768876658 2314 14-15 0.6538328717776238 2314 16-17 0.6506907782809961 2314 18-19 0.6814853425237501 2314 20-21 0.6205971573194162 2314 22-23 0.6324908169606758 2314 24-25 0.7093011880098317 2314 26-27 0.7797797153745663 2314 28-29 0.7012386488914544 2314 30-31 0.7036652415311337 2314 32-33 0.7773317261535269 2314 34-35 0.8227382041681679 2314 36-37 0.7850077721518645 2314 38-39 0.836841240284933 2314 40-41 0.8743185632828272 2314 42-43 0.781215661439937 2314 44-45 0.88880738866537 2314 46-47 0.7657482273110077 2314 48-49 0.6954395739168717 2314 50-51 0.7693651323954072 2314 52-53 0.8041632140212229 2314 54-55 0.8589780237964746 2314 56-57 0.7554385115567541 2314 58-59 0.7874818186657819 2314 60-61 0.6327039247098867 2314 62-63 0.7780013477938894 2314 64-65 0.7891452648827624 2314 66-67 0.74833028534524 2314 68-69 0.8177783839767514 2314 70-71 0.7510078925920638 2314 72-73 0.8494106491892026 2314 74-75 0.8935700551525727 2314 76-77 0.8868725579103653 2314 78-79 0.8937495590158591 2314 80-81 0.8806285730430652 2314 82-83 0.8831684629056546 2314 84-85 0.9510924996862897 2314 86-87 0.9566725822953828 2314 88-89 0.8850835354209394 2314 90-91 0.8496127626326526 2314 92-93 1.1088347685369513 2314 94-95 1.0858837664214889 2314 96-97 1.1038607373289118 2314 98-99 1.0864843097403813 2314 100 1.0989615762811589 2315 1 0.2852305404208124 2315 2 0.3228764945933875 2315 3 0.32983969557665915 2315 4 0.18927994194987718 2315 5 0.2323090204801801 2315 6 0.23345743456674484 2315 7 0.16325624014762496 2315 8 0.19664250312337828 2315 9 0.11355787367604364 2315 10-11 0.2530757116782567 2315 12-13 0.18922193968808187 2315 14-15 0.379167529171923 2315 16-17 0.2052594974986448 2315 18-19 0.3907542284949841 2315 20-21 0.2980102884598921 2315 22-23 0.2642555299985716 2315 24-25 0.3725542671420854 2315 26-27 0.5053703058851582 2315 28-29 0.39363242044010605 2315 30-31 0.4524025338631148 2315 32-33 0.4965071874933642 2315 34-35 0.4770362977277429 2315 36-37 0.6019923721607867 2315 38-39 0.5941345069861228 2315 40-41 0.6698928816321441 2315 42-43 0.6796538674757429 2315 44-45 0.5973712857784506 2315 46-47 0.6702855634231923 2315 48-49 0.6347775018846704 2315 50-51 0.6362046989041019 2315 52-53 0.30733105836509367 2315 54-55 0.44269898995415957 2315 56-57 0.3683238890698206 2315 58-59 0.40651891897127257 2315 60-61 0.5504862204577137 2315 62-63 0.5789924736106116 2315 64-65 0.5499712916786805 2315 66-67 0.48766054956427496 2315 68-69 0.5146845604344108 2315 70-71 0.47113931322592606 2315 72-73 0.5010094163758367 2315 74-75 0.28280731798835745 2315 76-77 0.4848568984145416 2315 78-79 0.5617620575515971 2315 80-81 0.6134814239454975 2315 82-83 0.5218810789357811 2315 84-85 0.6229786251187406 2315 86-87 0.6545971818185592 2315 88-89 0.4872331480214882 2315 90-91 0.6841968822006343 2315 92-93 0.7870682917423864 2315 94-95 0.7720347324469188 2315 96-97 0.8252828934964249 2315 98-99 0.9522601582812484 2315 100 0.943398279092655 2316 1 0.08020671875370411 2316 2 0.11719668570175656 2316 3 0.1076732216914742 2316 4 0.012210578825829543 2316 5 0.05501052353422864 2316 6 -0.11676856527125778 2316 7 -0.05237773321670147 2316 8 0.040625972420578194 2316 9 0.0828289176174053 2316 10-11 0.07446902239256303 2316 12-13 0.022852358051544286 2316 14-15 -0.07880298965395127 2316 16-17 0.140528068510946 2316 18-19 0.17508200829199438 2316 20-21 0.23373131961483296 2316 22-23 0.0847503231459612 2316 24-25 0.04866036629472603 2316 26-27 -0.020814654586544634 2316 28-29 0.0904308446620874 2316 30-31 -0.5577444809829117 2316 32-33 -0.3842040682937409 2316 34-35 -0.4249738921262036 2316 36-37 -0.22027762322280608 2316 38-39 -0.11651241012027214 2316 40-41 0.041323345473500694 2316 42-43 0.08003846541718929 2316 44-45 -0.029611727863454007 2316 46-47 -0.22862296612693456 2316 48-49 -0.13785810401163445 2316 50-51 0.03317406454624461 2316 52-53 -0.310645889137227 2316 54-55 -0.21446493350798335 2316 56-57 -0.22594548288989103 2316 58-59 -0.5647036685691802 2316 60-61 -0.1439195835515079 2316 62-63 -0.09954147103944422 2316 64-65 -0.44993349010287176 2316 66-67 -0.7108021680886765 2316 68-69 -0.7423800931988609 2316 70-71 -0.48160206127906946 2316 72-73 -0.6434192067728617 2316 74-75 -0.40012875801546244 2316 76-77 -0.11338911140228447 2316 78-79 -0.1611267744095457 2316 80-81 -0.18228791850963333 2316 82-83 0.022926867292117237 2316 84-85 0.12732398349843876 2316 86-87 0.20248381519616032 2316 88-89 0.2210617538725117 2316 90-91 -0.3569513840399523 2316 92-93 -0.34724476800255744 2316 94-95 -0.028994126940176557 2316 96-97 0.2174992472444579 2316 98-99 0.3768276995033055 2316 100 0.17368586340909786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 244.0 1 653.0 2 398.0 3 365.0 4 378.0 5 328.0 6 380.0 7 1033.0 8 2738.0 9 4550.0 10 6984.0 11 10519.0 12 15140.0 13 19321.0 14 22516.0 15 26565.0 16 32811.0 17 40659.0 18 49779.0 19 59610.0 20 72833.0 21 89318.0 22 97935.0 23 98798.0 24 97311.0 25 102045.0 26 112447.0 27 128749.0 28 151224.0 29 180248.0 30 219300.0 31 270525.0 32 341859.0 33 445878.0 34 617461.0 35 851250.0 36 1247853.0 37 1776307.0 38 1611846.0 39 388844.0 40 177.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.678768495107704 19.219849254975387 12.77579527005511 23.3255869798618 2 15.883814475340161 20.212590408015185 40.6959993956653 23.207595720979363 3 19.016583554598192 26.680920560406175 29.07957444069 25.222921444305634 4 12.578827350688718 16.31787737814215 38.42334532570082 32.67994994546831 5 13.300229897057447 37.974143649773474 34.26037092378597 14.465255529383105 6 30.426208494321056 36.891178701508295 18.578299179951156 14.1043136242195 7 27.80770895926657 32.36471683705104 22.334606774328996 17.492967429353396 8 26.568924404018706 34.834157683797265 20.244606552985232 18.352311359198794 9 27.296445790752582 15.733880838098205 20.653114132751345 36.31655923839787 10-11 25.316236162883392 26.09343037365993 27.879125819121736 20.71120764433494 12-13 25.703533107814657 23.865225235337668 28.23448373527844 22.19675792156923 14-15 23.21246228858099 25.175068483779217 26.050678730767835 25.561790496871957 16-17 22.24203147426808 28.074170886541317 26.86235370025613 22.821443938934472 18-19 21.958043654217043 27.36316949600796 29.040597776854575 21.638189072920415 20-21 22.971744716632678 26.55002389076549 28.777644008829657 21.700587383772174 22-23 23.042663785603285 26.28258737012534 28.502218341933812 22.172530502337565 24-25 22.524013871610787 26.392552136588844 28.887447767666842 22.19598622413353 26-27 22.355693795827438 26.683238527803844 28.641003128139147 22.32006454822957 28-29 22.09467606050702 26.895821153045656 28.78217683218586 22.22732595426146 30-31 22.839303483534167 26.623808605642658 28.656895014249006 21.879992896574173 32-33 22.713112890205217 26.860914504116636 28.310449981510903 22.115522624167248 34-35 22.751279211488267 26.77996257070071 28.607595583866992 21.86116263394403 36-37 22.64249803094856 26.645907826529424 28.43626805117913 22.275326091342883 38-39 22.599346406472954 26.828010047837914 28.56319018037865 22.009453365310485 40-41 22.588766551717423 26.353986157571228 28.809858126038318 22.24738916467303 42-43 22.400985635665652 26.46272385202954 28.42051068655888 22.715779825745923 44-45 22.211061864104373 26.893527463728383 28.497094136487462 22.398316535679783 46-47 22.321011508583773 26.648922238115325 28.130038048254292 22.90002820504661 48-49 22.56608929983801 26.735206538921403 28.116176171406256 22.58252798983434 50-51 22.58136368413117 26.77878181241016 27.818641929295623 22.82121257416305 52-53 22.362459252923156 27.13061319322118 27.671515797514466 22.8354117563412 54-55 22.250609825513237 26.930303689760503 27.90084642644452 22.918240058281732 56-57 22.630375945706213 26.82276817304265 27.537210193253724 23.009645687997413 58-59 22.263645005220653 26.81048064159521 28.02639002177683 22.899484331407308 60-61 22.52386279324254 27.184174895967843 27.61111099930755 22.68085131148207 62-63 22.51258527164757 26.913503060347292 27.61979479322122 22.954116874783917 64-65 22.109894030988947 26.752948932314073 27.737695965431975 23.399461071265005 66-67 22.376330542938973 26.603189473839944 27.55235035389766 23.46812962932342 68-69 22.030682904787284 26.805959296198218 27.733908370128496 23.429449428886002 70-71 22.30071749431661 26.455812629777707 27.829274396930913 23.41419547897477 72-73 22.486772961666922 26.761751533329015 27.533840781180075 23.217634723823988 74-75 22.50343783787547 26.91066386251216 27.404683422479785 23.181214877132582 76-77 22.321130336663682 26.70223195964746 27.497990885487134 23.47864681820172 78-79 22.267869531874126 26.70558005676699 27.510330345862187 23.516220065496693 80-81 22.310336917402065 26.947685840552232 27.359264707698276 23.382712534347423 82-83 22.776251551073557 26.540166475141184 27.700899550591856 22.982682423193403 84-85 22.766945842462995 26.717754156563316 27.237267115747887 23.278032885225798 86-87 22.264589977602583 26.962825381532816 27.582143076274363 23.190441564590238 88-89 22.605661511834477 26.470575275529633 27.480563641213063 23.44319957142283 90-91 22.838470252812254 26.321542333616076 27.5215779777544 23.318409435817266 92-93 22.969444701505168 26.41863924729138 27.346854237756197 23.265061813447254 94-95 22.572820631287147 26.239491251491692 27.564327234983022 23.623360882238142 96-97 22.751156660231374 26.18347333525634 27.45943106929581 23.60593893521647 98-99 22.570436480354243 26.327939257518256 27.31626523903633 23.785359023091175 100 23.023869011654174 26.102214891868208 26.859774319447393 24.014141777030225 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3020.0 1 2141.5 2 3159.5 3 4239.0 4 2670.0 5 2076.0 6 2812.5 7 4784.5 8 7378.0 9 8549.0 10 7477.0 11 6315.0 12 6508.0 13 7189.0 14 8022.5 15 8857.0 16 9589.0 17 10347.5 18 11192.5 19 11892.0 20 12791.0 21 14020.5 22 15435.0 23 17650.5 24 20324.5 25 23837.5 26 29472.0 27 36506.0 28 44776.0 29 58560.5 30 78008.5 31 96899.0 32 122738.5 33 153645.0 34 182525.5 35 211026.0 36 243540.5 37 279427.0 38 294175.5 39 292015.5 40 283617.0 41 275061.5 42 276479.0 43 287458.5 44 303018.0 45 326959.0 46 348931.5 47 360331.0 48 370613.5 49 375300.5 50 374728.5 51 372192.0 52 361684.5 53 345342.0 54 329333.0 55 301506.0 56 271369.0 57 237962.0 58 202649.5 59 176526.5 60 147486.0 61 117638.5 62 94385.0 63 75330.0 64 56753.5 65 40978.5 66 30124.5 67 22928.5 68 17820.0 69 13527.0 70 10473.5 71 8093.0 72 6483.5 73 5227.5 74 4114.0 75 3139.0 76 2243.5 77 1561.5 78 1043.5 79 707.5 80 503.0 81 364.0 82 268.5 83 197.5 84 149.5 85 118.0 86 88.0 87 66.5 88 43.5 89 33.0 90 38.5 91 31.5 92 17.0 93 15.0 94 13.5 95 8.0 96 7.5 97 6.0 98 3.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02517076159983404 2 0.11108841091382476 3 0.016994341417080172 4 0.0174836218801439 5 0.01912542965620219 6 0.04555744756082273 7 0.04609021962060323 8 0.03779419754687823 9 0.032422985352356416 10-11 0.03629373746014947 12-13 0.03546739712253072 14-15 0.030971453311934016 16-17 0.036783017923213195 18-19 0.036027351430259216 20-21 0.04671541132340688 22-23 0.046503389789412604 24-25 0.06559620074807722 26-27 0.057544818905884074 28-29 0.0495749838075349 30-31 0.04818325271259807 32-33 0.042659819929567536 34-35 0.04472567077361439 36-37 0.039860048390925086 38-39 0.04519320543831973 40-41 0.05442973329104501 42-43 0.05590301112982579 44-45 0.05892024065205212 46-47 0.06046962878508726 48-49 0.059246427627427926 50-51 0.05363601165096385 52-53 0.06388371912735416 54-55 0.049912043682089915 56-57 0.0548048483127272 58-59 0.052635704926478 60-61 0.04858554998222825 62-63 0.05528869232620133 64-65 0.052940146103495435 66-67 0.051004770049598906 68-69 0.056403164492068704 70-71 0.05718601323297068 72-73 0.05975201743926046 74-75 0.05031977740130968 76-77 0.05106457099508448 78-79 0.0605620484281104 80-81 0.06114374853419728 82-83 0.05170607204665692 84-85 0.05763180209931763 86-87 0.05487552215739196 88-89 0.0634488031601864 90-91 0.06879283310676024 92-93 0.07332139561489452 94-95 0.07717583837391878 96-97 0.07295715349239153 98-99 0.08153043449518597 100 0.12644094455484667 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9197179.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.99563524454942 #Duplication Level Percentage of deduplicated Percentage of total 1 71.33227328058098 37.08966862661607 2 15.52431805804186 16.143935583326325 3 5.856673716136891 9.135644108717807 4 2.654795685596774 5.521511524683734 5 1.4510434312447669 3.7723962487501157 6 0.8182567978912598 2.5527469199716166 7 0.5470335237986887 1.9910348898984014 8 0.334958652738761 1.393311034384825 9 0.25413389631851924 1.189246803862848 >10 1.113483157797846 9.90534877551033 >50 0.0605280126063667 2.174846884358095 >100 0.04618765550814479 4.757234165310683 >500 0.004563708735466486 1.5713600103367686 >1k 0.001594135235657931 1.3913819677053425 >5k 6.251510726258921E-5 0.2025556998040345 >10k+ 9.377266089388382E-5 1.2077767567629958 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33706 0.36648193973391185 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24851 0.2702024175021493 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15756 0.17131339946738017 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.0872899179193968E-5 0.0 0.0 0.0 0.0 6 4.349159671677587E-5 0.0 0.0 0.0 0.0 7 4.349159671677587E-5 0.0 0.0 0.0 0.0 8 4.349159671677587E-5 0.0 0.0 0.0 0.0 9 4.349159671677587E-5 0.0 0.0 3.2618697537581906E-5 0.0 10-11 4.8928046306372856E-5 0.0 0.0 3.2618697537581906E-5 0.0 12-13 5.436449589596984E-5 0.0 0.0 4.8928046306372856E-5 0.0 14-15 5.436449589596984E-5 0.0 0.0 5.436449589596984E-5 3.2618697537581906E-5 16-17 8.698319343355175E-5 0.0 0.0 8.154674384395477E-5 3.2618697537581906E-5 18-19 9.785609261274571E-5 0.0 0.0 1.3047479015032763E-4 3.2618697537581906E-5 20-21 9.785609261274571E-5 0.0 0.0 3.044411770174311E-4 3.2618697537581906E-5 22-23 9.785609261274571E-5 0.0 0.0 0.001038361871613024 3.2618697537581906E-5 24-25 1.0872899179193968E-4 0.0 0.0 0.002778025740284059 3.2618697537581906E-5 26-27 1.1960189097113365E-4 0.0 0.0 0.005322284148215447 3.2618697537581906E-5 28-29 1.1960189097113365E-4 0.0 0.0 0.011476345083639233 3.2618697537581906E-5 30-31 1.1960189097113365E-4 0.0 0.0 0.031504225371714524 3.2618697537581906E-5 32-33 1.1960189097113365E-4 0.0 0.0 0.07440868553281392 3.2618697537581906E-5 34-35 1.1960189097113365E-4 0.0 0.0 0.13450319929621898 3.2618697537581906E-5 36-37 1.1960189097113365E-4 0.0 0.0 0.2289234557683394 3.2618697537581906E-5 38-39 1.1960189097113365E-4 0.0 0.0 0.36899357944430566 3.2618697537581906E-5 40-41 1.304747901503276E-4 0.0 0.0 0.5235246590286 3.2618697537581906E-5 42-43 1.4134768932952158E-4 0.0 0.0 0.6747938688591361 3.805514712717889E-5 44-45 1.4134768932952158E-4 0.0 0.0 0.8506249579354712 4.349159671677587E-5 46-47 1.4134768932952158E-4 1.6309348768790953E-5 0.0 1.0543830885535663 4.349159671677587E-5 48-49 1.4134768932952158E-4 2.1745798358387936E-5 0.0 1.2753584550219148 4.349159671677587E-5 50-51 1.4134768932952158E-4 2.1745798358387936E-5 0.0 1.5136815321306676 4.349159671677587E-5 52-53 1.4678413891911857E-4 2.1745798358387936E-5 0.0 1.7450459537647358 4.349159671677587E-5 54-55 1.5222058850871556E-4 2.1745798358387936E-5 0.0 2.00155939120028 4.349159671677587E-5 56-57 1.739663868671035E-4 2.1745798358387936E-5 0.0 2.2844450455949588 4.349159671677587E-5 58-59 1.739663868671035E-4 2.1745798358387936E-5 0.0 2.6066036118248865 4.349159671677587E-5 60-61 1.739663868671035E-4 2.1745798358387936E-5 0.0 2.930675808310353 4.349159671677587E-5 62-63 1.739663868671035E-4 2.1745798358387936E-5 0.0 3.2627015305453986 4.349159671677587E-5 64-65 1.739663868671035E-4 2.1745798358387936E-5 0.0 3.5970866719023302 4.349159671677587E-5 66-67 1.739663868671035E-4 2.1745798358387936E-5 0.0 3.9734194582925917 4.349159671677587E-5 68-69 1.739663868671035E-4 2.1745798358387936E-5 0.0 4.3837246181682445 4.349159671677587E-5 70-71 1.739663868671035E-4 2.1745798358387936E-5 0.0 4.819189666744553 6.523739507516381E-5 72-73 1.739663868671035E-4 2.1745798358387936E-5 0.0 5.24320011603558 7.611029425435778E-5 74-75 1.739663868671035E-4 2.1745798358387936E-5 0.0 5.659191802181951 7.611029425435778E-5 76-77 1.739663868671035E-4 3.2618697537581906E-5 0.0 6.11703871371863 7.611029425435778E-5 78-79 1.739663868671035E-4 3.2618697537581906E-5 0.0 6.608232807037897 7.611029425435778E-5 80-81 1.739663868671035E-4 4.349159671677587E-5 0.0 7.115285023810018 7.611029425435778E-5 82-83 1.9027573563589443E-4 4.349159671677587E-5 0.0 7.6316226964811715 7.611029425435778E-5 84-85 1.9571218522549142E-4 4.349159671677587E-5 0.0 8.129416639602209 7.611029425435778E-5 86-87 1.9571218522549142E-4 4.349159671677587E-5 0.0 8.644324526031298 8.154674384395477E-5 88 1.9571218522549142E-4 4.349159671677587E-5 0.0 9.048502807219474 8.698319343355175E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17380 0.0 46.553787 1 GTATCAA 30455 0.0 40.252434 1 TACCTGG 6785 0.0 36.60489 2 ATCAACG 37015 0.0 32.487045 3 CAACGCA 37085 0.0 32.398975 5 TATCAAC 38225 0.0 31.674961 2 AACGCAG 39760 0.0 30.622034 6 TCAACGC 39330 0.0 30.574 4 GTACATG 45195 0.0 29.185286 1 TACATGG 47015 0.0 27.793987 2 ACCTGGG 8790 0.0 27.745731 3 ACGCAGA 44595 0.0 26.965675 7 ACATGGG 47425 0.0 26.654015 3 GTACTAG 3915 0.0 26.55442 1 CGCAGAG 45745 0.0 26.244972 8 GTACCTG 9675 0.0 26.109566 1 CATGGGG 29350 0.0 24.751827 4 GCAGAGT 49570 0.0 23.718569 9 ATGGGGG 20330 0.0 22.67412 5 TCTAACG 1850 0.0 22.629414 2 >>END_MODULE