FastQCFastQC Report
Mon 6 Jun 2016
ERR1211357_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211357_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9197179
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288580.31377012451317954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234640.25512170634060727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153870.16730129967025759No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172650.047.6785621
GTATCAA300400.039.5726781
TACCTGG67250.035.79222
CAACGCA375400.031.0430185
ATCAACG377750.030.8577863
TATCAAC393750.030.0291672
TCAACGC400350.029.2100934
AACGCAG405950.029.157416
GTACATG448800.028.2652841
ACCTGGG85750.027.5265313
TACATGG463500.027.0995432
ACATGGG461550.026.3532313
ACGCAGA455800.025.6288627
CGCAGAG467700.024.9266158
CATGGGG285150.024.619714
GTACCTG100350.024.3190251
ATGGGGG199900.023.0465185
GCAGAGT507950.022.7288999
TAGGCAT52950.022.1503545
TGGGGGG177400.021.5001376
Produced by FastQC (version 0.11.5)