##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211357_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9197179 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08840101948652 33.0 31.0 34.0 30.0 34.0 2 32.21631154509443 33.0 31.0 34.0 30.0 34.0 3 32.28384790597204 34.0 31.0 34.0 30.0 34.0 4 35.92970485841365 37.0 35.0 37.0 35.0 37.0 5 35.83539028652155 37.0 35.0 37.0 35.0 37.0 6 35.85290011208872 37.0 35.0 37.0 35.0 37.0 7 35.77006145036429 37.0 35.0 37.0 33.0 37.0 8 35.749996928405984 37.0 35.0 37.0 33.0 37.0 9 37.36163741077563 39.0 37.0 39.0 34.0 39.0 10-11 37.310030390840495 39.0 37.0 39.0 34.0 39.0 12-13 37.23420773913392 39.0 37.0 39.0 33.0 39.0 14-15 38.45304098137048 40.0 38.0 41.0 34.0 41.0 16-17 38.43820974888061 40.0 38.0 41.0 34.0 41.0 18-19 38.449603731752966 40.0 38.0 41.0 34.0 41.0 20-21 38.419314987780496 40.0 38.0 41.0 34.0 41.0 22-23 38.307354081072035 40.0 38.0 41.0 34.0 41.0 24-25 38.21336874056708 40.0 38.0 41.0 33.0 41.0 26-27 38.106998026242614 40.0 37.0 41.0 33.0 41.0 28-29 38.00333140194401 40.0 37.0 41.0 33.0 41.0 30-31 38.00566396500493 40.0 37.0 41.0 33.0 41.0 32-33 37.93360480425574 40.0 37.0 41.0 33.0 41.0 34-35 37.797285613338616 40.0 37.0 41.0 33.0 41.0 36-37 37.64734300593693 40.0 37.0 41.0 33.0 41.0 38-39 37.53208739331919 40.0 36.0 41.0 32.0 41.0 40-41 37.41623893587371 40.0 36.0 41.0 32.0 41.0 42-43 37.30491996513278 40.0 36.0 41.0 31.5 41.0 44-45 37.13519205182372 39.0 36.0 41.0 31.0 41.0 46-47 36.884185955280415 39.0 35.5 41.0 31.0 41.0 48-49 35.325062065226746 39.0 35.0 40.5 24.0 41.0 50-51 36.05039431112518 39.0 35.0 40.0 28.0 41.0 52-53 36.14317542368154 39.0 35.0 40.0 29.0 41.0 54-55 35.97196857862612 38.0 35.0 40.0 29.0 41.0 56-57 35.751560125120974 38.0 34.5 40.0 28.0 41.0 58-59 35.508673692226715 38.0 34.0 40.0 28.0 41.0 60-61 35.24920206511149 37.5 34.0 40.0 28.0 41.0 62-63 34.920082070817585 37.0 34.0 40.0 27.0 41.0 64-65 34.587027989778164 36.0 33.5 39.0 26.0 41.0 66-67 34.24884146541021 36.0 33.0 39.0 26.0 40.5 68-69 33.89028554299095 35.0 33.0 39.0 26.0 40.0 70-71 33.5443748566816 35.0 32.5 38.0 26.0 40.0 72-73 33.171184990528076 35.0 32.0 37.0 25.0 39.5 74-75 32.81208911993558 35.0 32.0 37.0 25.0 39.0 76-77 31.87660504378571 34.0 30.5 36.0 23.5 38.0 78-79 32.324839442616046 34.5 31.5 36.0 25.0 38.0 80-81 32.206518705355194 35.0 32.0 35.5 25.0 37.0 82-83 31.93901244066251 34.0 31.0 35.0 24.0 37.0 84-85 31.660744560913734 34.0 31.0 35.0 24.0 36.5 86-87 31.4109456823663 34.0 31.0 35.0 23.0 36.0 88-89 31.190146076313184 34.0 31.0 35.0 23.0 36.0 90-91 30.974651738321064 34.0 30.5 35.0 21.5 35.0 92-93 30.78361859652835 34.0 30.0 35.0 20.0 35.0 94-95 30.595103237634063 34.0 30.0 35.0 20.0 35.0 96-97 30.415358720320654 34.0 29.5 35.0 20.0 35.0 98-99 30.204077902582956 34.0 29.0 35.0 19.0 35.0 100 27.71679696567828 31.0 25.0 34.0 12.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.26575026751848085 1101 2 -0.23646287739585503 1101 3 -0.26308284347222965 1101 4 -0.13915545226895176 1101 5 -0.14666392891785307 1101 6 -0.22797052174087185 1101 7 -0.220991901814223 1101 8 -0.21160199925736123 1101 9 -0.3529874186569941 1101 10-11 -0.30379512422503296 1101 12-13 -0.41060158679525216 1101 14-15 -0.4605708424331425 1101 16-17 -0.5188829579011411 1101 18-19 -0.4617200265359003 1101 20-21 -0.5466076247251834 1101 22-23 -0.5734460090714677 1101 24-25 -0.5427652592135743 1101 26-27 -0.5743381517302097 1101 28-29 -0.5427659821916819 1101 30-31 -0.5205168790373591 1101 32-33 -0.5334951042659952 1101 34-35 -0.5759375366560064 1101 36-37 -0.5960817146572239 1101 38-39 -0.5784776565464114 1101 40-41 -0.5116403791977646 1101 42-43 -0.5949519286538774 1101 44-45 -0.6150021052487631 1101 46-47 -0.608306778264172 1101 48-49 0.6562325030730847 1101 50-51 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1102 72-73 -0.8244368330037588 1102 74-75 -0.7962305122317446 1102 76-77 -0.7748863347123525 1102 78-79 -0.603973146295175 1102 80-81 -0.5813779280066846 1102 82-83 -0.6021397990253554 1102 84-85 -0.6072413530700125 1102 86-87 -0.6396760257557688 1102 88-89 -0.724417078805029 1102 90-91 -0.7214757246651082 1102 92-93 -0.7450168080162207 1102 94-95 -0.7729841062588036 1102 96-97 -0.8549365565582647 1102 98-99 -0.9293089562539727 1102 100 -0.8882667313827142 1103 1 -0.31435971990686795 1103 2 -0.275805275715733 1103 3 -0.3399156933923706 1103 4 -0.1526649100447912 1103 5 -0.15810137016942605 1103 6 -0.22065828317115432 1103 7 -0.26682629322257867 1103 8 -0.2401887743571507 1103 9 -0.3969443739969023 1103 10-11 -0.394589831113322 1103 12-13 -0.45785624501417743 1103 14-15 -0.4905217092878331 1103 16-17 -0.5484752834021833 1103 18-19 -0.562463171881852 1103 20-21 -0.5962590755196828 1103 22-23 -0.6124997926999711 1103 24-25 -0.6648121641723321 1103 26-27 -0.6270110531935131 1103 28-29 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2311 4 0.2456191673120358 2311 5 0.30723060274255687 2311 6 0.37767912979688845 2311 7 0.3802995816244774 2311 8 0.19187583224824323 2311 9 0.47831985002805055 2311 10-11 0.353545886541923 2311 12-13 0.4620976280158189 2311 14-15 0.45180347174754587 2311 16-17 0.4664105686939024 2311 18-19 0.5965465839033897 2311 20-21 0.6388372230642219 2311 22-23 0.6659794885065153 2311 24-25 0.6310832454633442 2311 26-27 0.6551347708228406 2311 28-29 0.7298575460586818 2311 30-31 0.5886029534205903 2311 32-33 0.611308726011714 2311 34-35 0.7641406435570559 2311 36-37 0.8032577386444117 2311 38-39 0.8185638516874576 2311 40-41 0.7182328547560388 2311 42-43 0.7523542373313958 2311 44-45 0.7773899529819985 2311 46-47 0.7986274677236125 2311 48-49 -5.962692396968784 2311 50-51 -1.114284169634125 2311 52-53 0.25182542607932135 2311 54-55 0.6381131505681452 2311 56-57 0.8141125406477414 2311 58-59 0.852421194033262 2311 60-61 0.6909502827602694 2311 62-63 0.5627632673767593 2311 64-65 0.6428989707335546 2311 66-67 0.7777469814134932 2311 68-69 0.8290488773719389 2311 70-71 0.8253441659924619 2311 72-73 0.3636648544538801 2311 74-75 0.6304806967894692 2311 76-77 0.7624869310851317 2311 78-79 0.6452307325780922 2311 80-81 0.6781717549006672 2311 82-83 0.6982734973089357 2311 84-85 0.7549007578913347 2311 86-87 0.7651261162726506 2311 88-89 0.7885772435495326 2311 90-91 0.885201915288615 2311 92-93 0.8492869711308373 2311 94-95 0.8429293688216966 2311 96-97 0.9289169821256742 2311 98-99 0.9137425068060772 2311 100 1.1567312423400367 2312 1 0.05289724204951085 2312 2 0.24809851657040838 2312 3 0.22938421592046865 2312 4 0.029444768146014155 2312 5 0.19336776778161635 2312 6 0.11589586511538386 2312 7 0.28722210128314885 2312 8 0.34968548895763973 2312 9 0.2811793903017161 2312 10-11 0.21682078342834643 2312 12-13 0.32113708308629185 2312 14-15 0.38380997604902944 2312 16-17 0.5012300277163462 2312 18-19 0.3345610964053449 2312 20-21 0.3699916999384243 2312 22-23 0.6055231413257118 2312 24-25 0.7524381679131622 2312 26-27 0.6595261948417885 2312 28-29 0.7477959015346016 2312 30-31 0.7722756389717702 2312 32-33 0.8041910458530239 2312 34-35 0.41158255061949944 2312 36-37 0.7012412269966148 2312 38-39 0.8161276563170645 2312 40-41 0.5883683189769471 2312 42-43 0.6117309776656299 2312 44-45 0.7305360581649225 2312 46-47 0.840402414903231 2312 48-49 -4.647686047586092 2312 50-51 -0.7482098233778274 2312 52-53 0.5232957217418601 2312 54-55 0.7555482058520582 2312 56-57 0.897663091821201 2312 58-59 0.9665213444553302 2312 60-61 0.9119575208349175 2312 62-63 0.768389712993546 2312 64-65 0.9220114113557614 2312 66-67 0.8867326804861335 2312 68-69 0.8606608043386359 2312 70-71 0.8595140312996179 2312 72-73 0.9539405262559626 2312 74-75 0.690722221044517 2312 76-77 0.8609583617941077 2312 78-79 0.5005947983463059 2312 80-81 0.7311628367399621 2312 82-83 0.7528523537609111 2312 84-85 0.7272575972377595 2312 86-87 0.81674136448515 2312 88-89 0.8480269715801967 2312 90-91 0.6949654619966701 2312 92-93 0.9249607204171859 2312 94-95 0.9808197541627024 2312 96-97 1.0452649741579734 2312 98-99 1.0204828887584547 2312 100 0.5643136770800474 2313 1 0.08879153971447096 2313 2 0.2387317431482998 2313 3 0.3004382426754262 2313 4 0.06115127856202918 2313 5 0.09406758320911024 2313 6 0.23059347142162068 2313 7 0.19616435933306775 2313 8 0.26025764914191285 2313 9 0.4288123819287435 2313 10-11 0.24245667993388764 2313 12-13 0.021167092434339452 2313 14-15 0.16320900036421904 2313 16-17 0.08176949230889363 2313 18-19 0.41834726036882586 2313 20-21 0.3742413752841145 2313 22-23 0.4780284114039475 2313 24-25 0.2736518557167926 2313 26-27 0.4157944844189956 2313 28-29 0.4092752851524182 2313 30-31 0.5999598414149787 2313 32-33 0.4997417924607461 2313 34-35 0.38918075195405777 2313 36-37 -0.4003908569730399 2313 38-39 0.12289506198193578 2313 40-41 0.40117160623380954 2313 42-43 0.5128425646031403 2313 44-45 0.49052816085843887 2313 46-47 0.37330551531028533 2313 48-49 -5.24177162843543 2313 50-51 -1.342155841405166 2313 52-53 0.0654657466644295 2313 54-55 0.23693626551624902 2313 56-57 0.27327857940115763 2313 58-59 0.3804682588229724 2313 60-61 0.6751288630513912 2313 62-63 0.7581728743211897 2313 64-65 0.8358030977752406 2313 66-67 0.8477986193328135 2313 68-69 0.8438301667608812 2313 70-71 0.809543224846081 2313 72-73 0.5968376855445854 2313 74-75 0.7015091835215301 2313 76-77 0.7418397444521183 2313 78-79 0.7271907440415504 2313 80-81 -0.14622616008931288 2313 82-83 0.2827865959874707 2313 84-85 0.6419823003068146 2313 86-87 0.7775271709539631 2313 88-89 0.7809159048116605 2313 90-91 0.7996293597286162 2313 92-93 0.8574890259549477 2313 94-95 0.7346362649036493 2313 96-97 0.7785103449873532 2313 98-99 0.9566756014354993 2313 100 0.9888088451740558 2314 1 0.11802856932598615 2314 2 0.23432082752272976 2314 3 0.23729709322240922 2314 4 0.10058474253381178 2314 5 0.235328293424935 2314 6 0.29824593062897264 2314 7 0.14549147896881465 2314 8 0.24792906992801989 2314 9 0.45903119318916197 2314 10-11 0.3495967229519721 2314 12-13 0.2580540433073182 2314 14-15 0.32551804390575967 2314 16-17 0.4996365719104148 2314 18-19 0.5490173505056006 2314 20-21 0.5763235449247475 2314 22-23 0.3324695812689953 2314 24-25 0.48606777807771806 2314 26-27 0.5382523628726901 2314 28-29 0.648119942786515 2314 30-31 0.5242471217262121 2314 32-33 0.5181360734141265 2314 34-35 0.43239979538492435 2314 36-37 0.49355910858559326 2314 38-39 0.5362256929216755 2314 40-41 0.34342898084175033 2314 42-43 0.3783613608473573 2314 44-45 0.6203769461013806 2314 46-47 0.7023993096747247 2314 48-49 -6.335215187172441 2314 50-51 -1.5237752888877694 2314 52-53 -0.7947875683520849 2314 54-55 -0.4129568310142133 2314 56-57 -0.10966510072510971 2314 58-59 0.4294819840459283 2314 60-61 0.6813919190269075 2314 62-63 0.5439064776207445 2314 64-65 0.7117426150344386 2314 66-67 0.7929370954850938 2314 68-69 0.7946665388490786 2314 70-71 0.581723217009376 2314 72-73 0.7195746802463816 2314 74-75 0.7717002176279735 2314 76-77 0.6997768697623421 2314 78-79 0.5237510606488129 2314 80-81 0.5897500865799188 2314 82-83 0.6022399019735296 2314 84-85 0.6655624042390045 2314 86-87 0.7138471351258993 2314 88-89 0.618748089654634 2314 90-91 0.8429362597097274 2314 92-93 0.8884396407986479 2314 94-95 0.902055043197457 2314 96-97 0.8901462829132321 2314 98-99 0.9301762774702347 2314 100 -0.45702663109353026 2315 1 0.10440558544519973 2315 2 0.17316011054039393 2315 3 0.27479659371692833 2315 4 0.11738724224764496 2315 5 0.23906100609682568 2315 6 0.27234250159975915 2315 7 0.33635128098242717 2315 8 0.2685135916402146 2315 9 0.4431952882780905 2315 10-11 0.22077132867224236 2315 12-13 0.4113592958341812 2315 14-15 0.1713525098901414 2315 16-17 0.3227517239504465 2315 18-19 0.4396418329070073 2315 20-21 0.3502423090709428 2315 22-23 0.4213017197813187 2315 24-25 0.4392172968468486 2315 26-27 0.4812695043134525 2315 28-29 0.6054008554211094 2315 30-31 0.5603368157753792 2315 32-33 0.6008633421843257 2315 34-35 0.6527288418173214 2315 36-37 0.5489077832128046 2315 38-39 0.5855704933664114 2315 40-41 0.6194938745220426 2315 42-43 0.6734730679851211 2315 44-45 0.6452167615962807 2315 46-47 0.5525144802036621 2315 48-49 -4.812159426052283 2315 50-51 -0.9712662574986339 2315 52-53 0.24232549891100774 2315 54-55 0.09138532220811157 2315 56-57 0.47933147461601067 2315 58-59 0.5851410592958786 2315 60-61 0.4756845123658877 2315 62-63 0.1979519506949785 2315 64-65 0.41123964486602205 2315 66-67 -0.2843021797593721 2315 68-69 0.4623681095817176 2315 70-71 0.44864754593279343 2315 72-73 0.5369514991032247 2315 74-75 0.6913504529237784 2315 76-77 0.6518352964517824 2315 78-79 0.6044665849266337 2315 80-81 0.5031142892337002 2315 82-83 0.4891501564344658 2315 84-85 0.4738896040529994 2315 86-87 0.525915991297655 2315 88-89 0.5048704893552518 2315 90-91 0.5930438241372045 2315 92-93 0.6873621518103477 2315 94-95 0.24258732793591165 2315 96-97 0.36862669278652405 2315 98-99 0.36268813457952476 2315 100 0.468151027644339 2316 1 0.2280529977557748 2316 2 0.2509885954961888 2316 3 0.17104076160104142 2316 4 -0.03235974993383195 2316 5 0.14231746916774313 2316 6 0.11788334017118274 2316 7 0.21607851046476867 2316 8 0.2812185854778164 2316 9 0.4066001277543805 2316 10-11 0.32196741681219976 2316 12-13 0.29348428754454403 2316 14-15 0.41285854961695634 2316 16-17 0.4209281760237218 2316 18-19 0.13586766644793613 2316 20-21 0.4288137738986535 2316 22-23 0.5476119561134638 2316 24-25 0.5996883049495239 2316 26-27 0.5992532596125173 2316 28-29 0.6439630930594973 2316 30-31 0.582332943457601 2316 32-33 0.46047810388837007 2316 34-35 0.510583595857625 2316 36-37 0.45897224969986894 2316 38-39 0.5111150588242026 2316 40-41 0.1227666845720421 2316 42-43 0.07185966645158004 2316 44-45 0.35580979501212084 2316 46-47 0.3740795482817063 2316 48-49 -5.05650783045601 2316 50-51 -1.1114393628640045 2316 52-53 0.19259395674936286 2316 54-55 0.44338475103106845 2316 56-57 0.39593994915649944 2316 58-59 0.27794330269596657 2316 60-61 0.41020470935037423 2316 62-63 0.3134542992153868 2316 64-65 0.3615872211242177 2316 66-67 0.47440617030961363 2316 68-69 0.22455626888293523 2316 70-71 -0.16340003794584135 2316 72-73 -0.053148194487228295 2316 74-75 0.15841321211296844 2316 76-77 0.12994136838976544 2316 78-79 0.22753182521259419 2316 80-81 0.15802953170956613 2316 82-83 0.06621935504708532 2316 84-85 0.24923234045132858 2316 86-87 0.293720599446079 2316 88-89 0.1744354904052301 2316 90-91 0.2071053832858496 2316 92-93 0.2379447140329063 2316 94-95 0.4046371661996915 2316 96-97 0.5231811840367726 2316 98-99 0.44838620109745975 2316 100 -0.3191489899162221 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 8.0 11 12.0 12 45.0 13 250.0 14 867.0 15 2671.0 16 6441.0 17 13044.0 18 22013.0 19 31850.0 20 44016.0 21 60391.0 22 73830.0 23 75975.0 24 77046.0 25 84917.0 26 100260.0 27 123145.0 28 151599.0 29 185915.0 30 227386.0 31 282499.0 32 356690.0 33 464995.0 34 650742.0 35 905368.0 36 1400274.0 37 2038042.0 38 1606836.0 39 210039.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.86818062328544 19.932237065424665 13.578428712150767 23.621153599139127 2 15.610919998025444 20.639800073893678 40.241011251714966 23.50826867636592 3 18.59033811487664 26.586275995768265 29.718859170810152 25.104526718544946 4 12.560710528653999 16.74608423275562 38.238756011510084 32.454449227080296 5 13.135348532918451 38.16951949285957 34.33380409577459 14.36132787844739 6 30.573356305597216 37.13822870450045 18.256067241495163 14.032347748407176 7 27.76553670012803 32.43863468945627 22.21166825682886 17.584160353586835 8 26.655971666731364 35.103379743935434 20.00888132975514 18.231767259578067 9 27.11987504439321 15.66712257832724 20.552702815263565 36.660299562015986 10-11 25.305093106015995 26.254634696410633 27.783556490466882 20.65671570710649 12-13 25.577290194350493 23.89723580807036 28.304519677107287 22.22095432047186 14-15 23.04548362802826 25.323696162290855 25.97523922177553 25.655580987905353 16-17 22.166564823412497 28.17218178694681 26.81893501675521 22.842318372885483 18-19 21.891051122648953 27.381964855299064 29.07047228410112 21.65651173795087 20-21 22.868803432882444 26.57180704192779 28.79520317610778 21.764186349081985 22-23 22.86259842650246 26.33002957599549 28.58240013256274 22.224971864939313 24-25 22.43697712896033 26.403226772736893 28.92456022103304 22.235235877269734 26-27 22.291818490954693 26.699374182663206 28.6937153782861 22.315091948096004 28-29 22.032577695771163 26.935503512524072 28.86099842450166 22.170920367203102 30-31 22.757222434913903 26.589298845604294 28.787390318054946 21.866088401426868 32-33 22.674447275238364 26.873160635633 28.358048848330707 22.094343240797926 34-35 22.66584111618746 26.826227297203232 28.6868641611496 21.821067425459713 36-37 22.59061614503477 26.645501343930565 28.53750414821076 22.226378362823898 38-39 22.497903953063776 26.815904737269985 28.67905515277367 22.007136156892575 40-41 22.45496373449113 26.369985935256512 28.95014056588438 22.224909764367975 42-43 22.342835094656163 26.464798615725897 28.550783819059234 22.641582470558706 44-45 22.126177027553823 26.874243247196038 28.589024032865158 22.410555692384982 46-47 22.24453352048436 26.64139555104545 28.226903511017344 22.887167417452847 48-49 22.615399506614363 26.774499233827605 28.103611754334214 22.506489505223822 50-51 22.498066054876464 26.747074124676075 27.87226125938854 22.882598561058916 52-53 22.256903958252725 27.071274474684525 27.76877158251592 22.903049984546826 54-55 22.192650717083172 26.896248980438227 27.927538231731646 22.983562070746956 56-57 22.594257251968287 26.7797478405012 27.556405708006633 23.069589199523886 58-59 22.12126383765593 26.79347400295017 28.08978843231199 22.995473727081908 60-61 22.3799540445254 27.236458444786017 27.63041377324631 22.75317373744227 62-63 22.41096641439902 26.980534094679665 27.554050226958243 23.054449263963072 64-65 21.956227299536526 26.766891765690897 27.67998619314835 23.596894741624226 66-67 22.162445176807058 26.686079478328406 27.54704512567835 23.604430219186185 68-69 21.814509837978793 26.841681408811592 27.720429439755712 23.623379313453903 70-71 22.012093068074407 26.470933383391216 27.829179431734996 23.68779411679938 72-73 22.154885893534274 26.816082517414525 27.527573794300043 23.501457794751158 74-75 22.120213368071887 26.933924077198313 27.378553257473236 23.567309297256564 76-77 21.887452308572307 26.746798958441513 27.480642051537462 23.88510668144872 78-79 21.78266005533774 26.707431731845265 27.52674908373477 23.983159129082228 80-81 21.743871381484308 27.065003402220782 27.316815582236426 23.87430963405848 82-83 22.140467681884974 26.61190668111153 27.685343040571126 23.562282596432375 84-85 22.10365088556649 26.779481330669764 27.234313331357967 23.882554452405774 86-87 21.55655755564663 27.07924972921582 27.513722459581118 23.850470255556424 88-89 21.731822591013824 26.594478433288526 27.498464642287622 24.17523433341003 90-91 21.88654505212324 26.391159239461953 27.59135799475097 24.13093771366384 92-93 21.946806517972707 26.56449858538385 27.362784136399064 24.12591076024438 94-95 21.40351794769639 26.355368694471398 27.662012923889794 24.579100433942415 96-97 21.489537895492365 26.31841479792177 27.614166967222182 24.577880339363688 98-99 21.235196425479938 26.50037756564118 27.49305875147568 24.771367257403202 100 21.267067890438327 26.34721257811725 27.376234953020884 25.009484578423542 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2823.0 1 2035.5 2 2731.5 3 3583.5 4 2371.5 5 1914.0 6 2581.5 7 4434.0 8 6592.0 9 7492.5 10 6699.5 11 5763.0 12 5925.5 13 6635.0 14 7491.5 15 8367.5 16 9376.5 17 10364.0 18 11252.0 19 12115.0 20 13246.5 21 14544.0 22 16183.0 23 18549.5 24 21225.0 25 24707.5 26 30715.5 27 37883.5 28 45836.0 29 59648.0 30 79042.5 31 97739.0 32 123520.0 33 154068.0 34 182483.5 35 211881.0 36 244837.5 37 279759.5 38 294342.0 39 292450.0 40 284385.0 41 276231.5 42 278285.5 43 289794.5 44 306088.5 45 331521.0 46 353977.0 47 365721.5 48 376592.0 49 380917.5 50 378949.0 51 373594.0 52 361658.0 53 345023.0 54 328773.0 55 301412.5 56 269797.0 57 234432.0 58 199494.0 59 174676.0 60 144763.5 61 113930.5 62 91143.0 63 71602.5 64 53099.5 65 37812.5 66 27098.0 67 20410.0 68 15548.0 69 11543.5 70 9021.5 71 7083.5 72 5661.0 73 4588.0 74 3500.0 75 2550.5 76 1751.0 77 1083.5 78 657.5 79 410.0 80 279.0 81 193.0 82 122.5 83 71.0 84 51.0 85 35.0 86 17.5 87 10.5 88 6.0 89 3.5 90 3.5 91 3.0 92 2.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030139676524725677 2 0.0019679947514341083 3 9.78560926127457E-5 4 0.02294181726809927 5 4.566617655261466E-4 6 1.0872899179193968E-5 7 4.349159671677587E-5 8 0.0038924979061514407 9 0.010361872917771851 10-11 0.0011905824601217395 12-13 7.067384466476079E-5 14-15 1.3591123973992462E-4 16-17 4.2404306798856473E-4 18-19 3.2618697537581906E-5 20-21 1.0872899179193968E-4 22-23 2.6638602989025224E-4 24-25 2.1745798358387936E-5 26-27 7.067384466476079E-5 28-29 9.676880269482631E-4 30-31 0.0026747331980817163 32-33 0.009758427013326586 34-35 0.018467619255860955 36-37 0.02119671694983864 38-39 0.02058783459580378 40-41 0.004610109251978242 42-43 0.004843876584330912 44-45 0.00885053993186389 46-47 0.01131868804554092 48-49 1.2044725888231598 50-51 0.017369456438762365 52-53 0.018016393939924404 54-55 0.008981014722014218 56-57 0.007159804109499228 58-59 0.008268839825777013 60-61 0.004903677529816479 62-63 0.007214168605395198 64-65 0.018967772618103878 66-67 0.029742815704685097 68-69 0.029731942805505906 70-71 0.027073518956192984 72-73 0.023115783654966374 74-75 0.009394184890823588 76-77 0.014613176496836692 78-79 0.008073127640551521 80-81 0.002663860298902522 82-83 0.01404234928992901 84-85 0.024415095106880055 86-87 0.023110347205376778 88-89 0.0333526182321775 90-91 0.021223899197786625 92-93 0.013281246347385433 94-95 0.0061649338346029805 96-97 0.0034793277373420697 98-99 0.015515627128709792 100 0.03595667758559445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9197179.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.05899470404385 #Duplication Level Percentage of deduplicated Percentage of total 1 71.10556967593926 37.01684475187743 2 15.820935926464767 16.472440392176928 3 5.757140671515814 8.991328671866315 4 2.6868931980186965 5.59507835063947 5 1.3987419620810457 3.6408550198150538 6 0.8241280531675711 2.574196677318273 7 0.5433871283100541 1.980173134445709 8 0.37526032374444035 1.5628540165159681 9 0.2601971668410141 1.2191042637525213 >10 1.1162038378307892 9.916321830543136 >50 0.06006151378661532 2.152704323507124 >100 0.045280673982694794 4.668494100726882 >500 0.0043837716688056505 1.5262943355599419 >1k 0.0016595365926348718 1.3967270424133367 >5k 6.262402233290358E-5 0.18287775787703744 >10k+ 9.393603349935537E-5 1.1037053309649996 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28858 0.31377012451317954 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23464 0.25512170634060727 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15387 0.16730129967025759 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.2618697537581906E-5 0.0 0.0 0.0 0.0 7 3.2618697537581906E-5 0.0 0.0 0.0 0.0 8 3.2618697537581906E-5 0.0 0.0 0.0 0.0 9 3.2618697537581906E-5 0.0 0.0 3.2618697537581906E-5 0.0 10-11 3.805514712717889E-5 0.0 0.0 3.2618697537581906E-5 0.0 12-13 4.349159671677587E-5 0.0 0.0 4.8928046306372856E-5 0.0 14-15 4.349159671677587E-5 0.0 0.0 5.436449589596984E-5 0.0 16-17 9.785609261274571E-5 0.0 0.0 8.698319343355175E-5 0.0 18-19 9.785609261274571E-5 0.0 0.0 1.5222058850871556E-4 1.0872899179193968E-5 20-21 9.785609261274571E-5 0.0 0.0 3.31623424965416E-4 1.0872899179193968E-5 22-23 1.0872899179193968E-4 0.0 0.0 0.001038361871613024 1.0872899179193968E-5 24-25 1.0872899179193968E-4 0.0 0.0 0.002810644437821641 1.0872899179193968E-5 26-27 1.0872899179193968E-4 0.0 0.0 0.005344029946573835 1.0872899179193968E-5 28-29 1.0872899179193968E-4 0.0 0.0 0.01159051052502077 1.0872899179193968E-5 30-31 1.0872899179193968E-4 0.0 0.0 0.0317216833552984 1.0872899179193968E-5 32-33 1.0872899179193968E-4 0.0 0.0 0.07489252954628806 1.0872899179193968E-5 34-35 1.0872899179193968E-4 0.0 0.0 0.13529148448671052 1.0872899179193968E-5 36-37 1.1960189097113365E-4 0.0 0.0 0.22870599778475553 1.0872899179193968E-5 38-39 1.3047479015032763E-4 0.0 0.0 0.36296999329903223 1.0872899179193968E-5 40-41 1.3047479015032763E-4 0.0 0.0 0.5107272566946887 1.0872899179193968E-5 42-43 1.4134768932952158E-4 0.0 0.0 0.6548747175628526 1.0872899179193968E-5 44-45 1.4134768932952158E-4 0.0 0.0 0.8226489883474053 1.0872899179193968E-5 46-47 1.4678413891911857E-4 5.436449589596984E-6 0.0 1.0180241136983417 1.0872899179193968E-5 48-49 1.5765703809831255E-4 1.6309348768790953E-5 0.0 1.2315515442289424 1.0872899179193968E-5 50-51 1.630934876879095E-4 2.1745798358387936E-5 0.0 1.4697441465475447 1.0872899179193968E-5 52-53 1.630934876879095E-4 2.1745798358387936E-5 0.0 1.7009998391898211 2.1745798358387936E-5 54-55 1.630934876879095E-4 2.1745798358387936E-5 0.0 1.957246890595475 3.2618697537581906E-5 56-57 1.630934876879095E-4 2.1745798358387936E-5 0.0 2.239877031859443 3.805514712717889E-5 58-59 1.630934876879095E-4 2.1745798358387936E-5 0.0 2.562122581282804 4.349159671677587E-5 60-61 1.630934876879095E-4 2.1745798358387936E-5 0.0 2.8861621590707323 4.349159671677587E-5 62-63 1.630934876879095E-4 2.1745798358387936E-5 0.0 3.217301740022674 5.436449589596984E-5 64-65 1.630934876879095E-4 2.1745798358387936E-5 0.0 3.55068113820553 5.436449589596984E-5 66-67 1.630934876879095E-4 2.1745798358387936E-5 0.0 3.926742102116312 5.980094548556682E-5 68-69 1.630934876879095E-4 2.1745798358387936E-5 0.0 4.33657972732726 6.523739507516381E-5 70-71 1.630934876879095E-4 2.1745798358387936E-5 0.0 4.771637042184349 6.523739507516381E-5 72-73 1.630934876879095E-4 2.1745798358387936E-5 0.0 5.196044352295416 6.523739507516381E-5 74-75 1.630934876879095E-4 2.1745798358387936E-5 0.0 5.613226620901909 6.523739507516381E-5 76-77 1.630934876879095E-4 2.1745798358387936E-5 0.0 6.0724435177351666 6.523739507516381E-5 78-79 1.630934876879095E-4 2.1745798358387936E-5 0.0 6.563452771768387 6.523739507516381E-5 80-81 1.630934876879095E-4 2.1745798358387936E-5 0.0 7.070526734338866 6.523739507516381E-5 82-83 1.739663868671035E-4 2.1745798358387936E-5 0.0 7.588141972663575 6.523739507516381E-5 84-85 1.7940283645670045E-4 2.1745798358387936E-5 0.0 8.08702864215212 6.523739507516381E-5 86-87 1.8483928604629744E-4 2.1745798358387936E-5 0.0 8.603447861567117 6.523739507516381E-5 88 1.8483928604629744E-4 2.1745798358387936E-5 0.0 9.008925454207208 6.523739507516381E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17265 0.0 47.678562 1 GTATCAA 30040 0.0 39.572678 1 TACCTGG 6725 0.0 35.7922 2 CAACGCA 37540 0.0 31.043018 5 ATCAACG 37775 0.0 30.857786 3 TATCAAC 39375 0.0 30.029167 2 TCAACGC 40035 0.0 29.210093 4 AACGCAG 40595 0.0 29.15741 6 GTACATG 44880 0.0 28.265284 1 ACCTGGG 8575 0.0 27.526531 3 TACATGG 46350 0.0 27.099543 2 ACATGGG 46155 0.0 26.353231 3 ACGCAGA 45580 0.0 25.628862 7 CGCAGAG 46770 0.0 24.926615 8 CATGGGG 28515 0.0 24.61971 4 GTACCTG 10035 0.0 24.319025 1 ATGGGGG 19990 0.0 23.046518 5 GCAGAGT 50795 0.0 22.728899 9 TAGGCAT 5295 0.0 22.150354 5 TGGGGGG 17740 0.0 21.500137 6 >>END_MODULE