FastQCFastQC Report
Mon 6 Jun 2016
ERR1211356_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211356_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13203219
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390580.2958217992142674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286910.21730306828963453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183110.1386858765275347No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204900.047.3576931
GTATCAA374950.041.510381
ATCAACG454300.033.327253
CAACGCA455750.033.210785
TATCAAC478250.032.1510282
TCAACGC485950.031.3495664
AACGCAG493350.030.9751726
TACCTGG97250.029.7454432
GTACATG557500.028.2386631
GTACTAG52950.027.8042811
ACGCAGA556000.027.3334887
TACATGG567600.027.1479112
CGCAGAG571650.026.509198
ACATGGG570800.025.9982933
CATGGGG357400.023.9159834
GCAGAGT622350.023.8376359
TAGTACT73250.023.158464
GTACCTG130650.022.969131
ACCTGGG122800.022.9216123
CTAGTAC73100.022.1777973
Produced by FastQC (version 0.11.5)