##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211356_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13203219 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45359779308364 31.0 31.0 34.0 29.0 34.0 2 31.459821048185294 31.0 31.0 34.0 28.0 34.0 3 31.517898703338936 31.0 31.0 34.0 28.0 34.0 4 35.13691009745426 37.0 35.0 37.0 32.0 37.0 5 35.18148044048955 37.0 35.0 37.0 32.0 37.0 6 35.246338942041334 37.0 35.0 37.0 32.0 37.0 7 35.1794438159361 37.0 35.0 37.0 32.0 37.0 8 35.180577554609975 37.0 35.0 37.0 32.0 37.0 9 36.74971762567901 39.0 37.0 39.0 32.0 39.0 10-11 36.677169749286136 39.0 37.0 39.0 32.0 39.0 12-13 36.59348171078583 39.0 37.0 39.0 32.0 39.0 14-15 37.736438439747154 40.0 37.0 41.0 32.0 41.0 16-17 37.740016203624286 40.0 37.0 41.0 32.0 41.0 18-19 37.736974180311634 40.0 37.0 41.0 32.0 41.0 20-21 37.69897462126471 40.0 37.0 41.0 32.0 41.0 22-23 37.57393174346347 40.0 37.0 41.0 32.0 41.0 24-25 37.461067183692094 40.0 37.0 41.0 31.0 41.0 26-27 37.31913028178961 40.0 37.0 41.0 31.0 41.0 28-29 37.18720181040699 40.0 36.0 41.0 31.0 41.0 30-31 37.00860899906303 39.0 36.0 41.0 30.0 41.0 32-33 36.89273763466318 39.0 36.0 41.0 30.0 41.0 34-35 36.750197660131214 39.0 35.5 41.0 30.0 41.0 36-37 36.65665744088619 39.0 35.0 41.0 30.0 41.0 38-39 36.50368194301708 39.0 35.0 41.0 30.0 41.0 40-41 36.335314895556905 39.0 35.0 40.0 29.0 41.0 42-43 36.18598744745505 39.0 35.0 40.0 28.5 41.0 44-45 36.0484710584593 39.0 35.0 40.0 28.0 41.0 46-47 35.88712741188342 39.0 35.0 40.0 27.0 41.0 48-49 35.65307585218423 38.0 34.5 40.0 26.5 41.0 50-51 34.981079841211454 37.5 33.5 39.5 25.5 40.5 52-53 35.22024284380953 38.0 34.0 40.0 26.5 40.5 54-55 35.57615324717404 38.0 34.0 40.0 27.0 41.0 56-57 35.47272937758588 38.0 34.0 40.0 26.5 41.0 58-59 35.24313904056276 38.0 34.0 40.0 26.0 41.0 60-61 34.9712436035485 37.5 34.0 40.0 26.0 41.0 62-63 34.69366034904064 37.0 33.5 40.0 26.0 41.0 64-65 34.41081894498607 36.5 33.0 40.0 26.0 41.0 66-67 34.1903508152065 36.0 33.0 39.0 25.5 41.0 68-69 33.848801909594926 35.5 33.0 39.0 25.0 41.0 70-71 33.511480344300885 35.0 32.5 39.0 24.5 40.5 72-73 33.15075664502724 35.0 32.0 37.5 24.0 40.0 74-75 32.77965843026614 35.0 32.0 37.0 23.0 39.5 76-77 32.43110661119837 35.0 31.5 36.5 23.0 39.0 78-79 32.06595800614986 35.0 31.0 36.0 22.0 39.0 80-81 31.7324950832066 35.0 31.0 36.0 20.5 37.5 82-83 31.419547119531988 34.0 31.0 35.0 20.0 37.0 84-85 31.11804560690844 34.0 30.0 35.0 20.0 37.0 86-87 30.86755199622153 34.0 30.0 35.0 19.5 36.0 88-89 30.60821645085187 34.0 30.0 35.0 19.0 36.0 90-91 30.347101793888292 34.0 29.0 35.0 18.0 36.0 92-93 30.10316851519315 34.0 29.0 35.0 18.0 35.0 94-95 29.879799161098518 34.0 29.0 35.0 17.0 35.0 96-97 29.67669925796126 34.0 29.0 35.0 16.0 35.0 98-99 29.432811157642693 34.0 28.0 35.0 15.0 35.0 100 27.719929662607278 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3709512587137169 1101 2 -0.3956975933724749 1101 3 -0.39184942851439786 1101 4 -0.29462867136384574 1101 5 -0.32440885581253553 1101 6 -0.34290793510924544 1101 7 -0.39096390041454754 1101 8 -0.4799720214423786 1101 9 -0.481211830339376 1101 10-11 -0.5329765742804611 1101 12-13 -0.4610912096649713 1101 14-15 -0.5581827372129098 1101 16-17 -0.5955086427130851 1101 18-19 -0.6549958831385254 1101 20-21 -0.7053799710087532 1101 22-23 -0.700399078715229 1101 24-25 -0.6665028588034332 1101 26-27 -0.6846752923137771 1101 28-29 -0.6826135000831997 1101 30-31 -0.8024102035611804 1101 32-33 -0.832140830978183 1101 34-35 -0.8726438404984904 1101 36-37 -0.9196527470712326 1101 38-39 -0.9451086953387531 1101 40-41 -0.9389398002715197 1101 42-43 -1.007250764523647 1101 44-45 -1.0313538711293546 1101 46-47 -0.9702295251533357 1101 48-49 -0.9239348861590884 1101 50-51 -0.9174593737148413 1101 52-53 -0.7570857174812105 1101 54-55 -0.7225878133020984 1101 56-57 -0.6882011154516903 1101 58-59 -0.7921515680814863 1101 60-61 -0.8899369786851352 1101 62-63 -0.813380552950207 1101 64-65 -0.8389369309090213 1101 66-67 -0.8938919245258532 1101 68-69 -0.8474132859460681 1101 70-71 -0.8406740820977134 1101 72-73 -1.0202704180500533 1101 74-75 -0.9442504618762868 1101 76-77 -0.925235695513642 1101 78-79 -0.916308098074321 1101 80-81 -0.9501529257501424 1101 82-83 -1.0546148848427919 1101 84-85 -1.0018337287242325 1101 86-87 -1.0932233556204665 1101 88-89 -1.1328082870784684 1101 90-91 -1.069519976179052 1101 92-93 -1.1030028714901512 1101 94-95 -1.1363065554722986 1101 96-97 -1.1912831628907696 1101 98-99 -1.1795247618949212 1101 100 -1.2722618603731917 1102 1 -0.3774908172790461 1102 2 -0.33698932747244825 1102 3 -0.40659613619447654 1102 4 -0.24176041794590475 1102 5 -0.2270973114742887 1102 6 -0.2779239023480571 1102 7 -0.3649163744442063 1102 8 -0.2917161833497275 1102 9 -0.4122539644601275 1102 10-11 -0.36064988993785363 1102 12-13 -0.40767805871657004 1102 14-15 -0.5382475580132535 1102 16-17 -0.5615689285120808 1102 18-19 -0.5969304692366109 1102 20-21 -0.6042728505657067 1102 22-23 -0.6681147830068355 1102 24-25 -0.6710506364910032 1102 26-27 -0.6308260829663794 1102 28-29 -0.7877496726698254 1102 30-31 -0.6743698495333632 1102 32-33 -0.7196802029653568 1102 34-35 -0.6699510234645416 1102 36-37 -0.7530048996129679 1102 38-39 -0.8844365170536648 1102 40-41 -0.8840561049686926 1102 42-43 -0.7767133222372351 1102 44-45 -0.8114618777716913 1102 46-47 -0.9025062428837103 1102 48-49 -0.9088740620946965 1102 50-51 -0.9024230026107105 1102 52-53 -0.775372710859692 1102 54-55 -0.7267522781701743 1102 56-57 -0.7186330628257664 1102 58-59 -0.7726125531112871 1102 60-61 -0.7571939131636967 1102 62-63 -0.7644142150491788 1102 64-65 -0.8082687825768815 1102 66-67 -0.7218493674420827 1102 68-69 -0.7974355150568968 1102 70-71 -0.8908341072990851 1102 72-73 -0.878621392983753 1102 74-75 -0.7948259509420552 1102 76-77 -0.8636517952676073 1102 78-79 -0.9145807507205639 1102 80-81 -0.9518631841817147 1102 82-83 -1.002765672417656 1102 84-85 -1.0387219219454806 1102 86-87 -0.9796574763241033 1102 88-89 -1.1239308228328078 1102 90-91 -1.2120548702530698 1102 92-93 -1.1837497853783105 1102 94-95 -1.1906270857761214 1102 96-97 -1.2805329471961429 1102 98-99 -1.2800110511407503 1102 100 -1.2124267915552807 1103 1 -0.4117863842925651 1103 2 -0.43256889369785156 1103 3 -0.4035196165886532 1103 4 -0.343093333148758 1103 5 -0.27859583872548654 1103 6 -0.37609128757038235 1103 7 -0.3294871757246369 1103 8 -0.3898320753426674 1103 9 -0.5419612255269186 1103 10-11 -0.5549373156550388 1103 12-13 -0.5829521665596076 1103 14-15 -0.651912885643732 1103 16-17 -0.6884787596382367 1103 18-19 -0.7082709048459819 1103 20-21 -0.7882127823266387 1103 22-23 -0.7295770947681604 1103 24-25 -0.7694834498321583 1103 26-27 -0.7541207505991849 1103 28-29 -0.7617025797544201 1103 30-31 -0.7330337991098332 1103 32-33 -0.8679304305921178 1103 34-35 -0.9320994957709345 1103 36-37 -0.9751319040782533 1103 38-39 -0.9760299320625094 1103 40-41 -0.9585240421190022 1103 42-43 -0.9799432399763077 1103 44-45 -0.997613210294297 1103 46-47 -0.9384263932409098 1103 48-49 -0.9718935286575885 1103 50-51 -0.972786499637948 1103 52-53 -0.8140057790506461 1103 54-55 -0.8625584817567358 1103 56-57 -0.8590998584240808 1103 58-59 -0.8993140390236789 1103 60-61 -0.8620187726699484 1103 62-63 -0.9604906894222083 1103 64-65 -0.8978610406327547 1103 66-67 -0.8468102093953362 1103 68-69 -0.8705060370733477 1103 70-71 -0.8843267167549413 1103 72-73 -1.0185563622040874 1103 74-75 -0.907849989291222 1103 76-77 -0.9349307636955757 1103 78-79 -0.9529075425795952 1103 80-81 -0.938279192206469 1103 82-83 -1.0274797470527943 1103 84-85 -0.9943111527391046 1103 86-87 -1.179019770498602 1103 88-89 -1.2080456610411403 1103 90-91 -1.0570327562075548 1103 92-93 -1.036705006368848 1103 94-95 -1.334220003193927 1103 96-97 -1.3949515774192456 1103 98-99 -1.4382050541334515 1103 100 -1.4558902664845341 1104 1 -0.34258868449356683 1104 2 -0.3649757951854262 1104 3 -0.432790386150657 1104 4 -0.2496477926445948 1104 5 -0.20589111198750487 1104 6 -0.2826682075479354 1104 7 -0.2405369528402872 1104 8 -0.29074808410961595 1104 9 -0.35548849771166147 1104 10-11 -0.3524341579928958 1104 12-13 -0.4075730075337134 1104 14-15 -0.5559184342893957 1104 16-17 -0.5229905790410427 1104 18-19 -0.5651218810314518 1104 20-21 -0.5526070394254603 1104 22-23 -0.5195504386655116 1104 24-25 -0.6439722177189751 1104 26-27 -0.6299664868803063 1104 28-29 -0.5563341147779042 1104 30-31 -0.6673392526091462 1104 32-33 -0.7099482304391032 1104 34-35 -0.6734771365065981 1104 36-37 -0.7082032023896332 1104 38-39 -0.7159061850544219 1104 40-41 -0.7192632151716865 1104 42-43 -0.7501048467910607 1104 44-45 -0.8387901352607798 1104 46-47 -0.8684459673996159 1104 48-49 -0.7342640646571112 1104 50-51 -0.6764410241991499 1104 52-53 -0.5893143301946324 1104 54-55 -0.5841823094220473 1104 56-57 -0.5502152090893304 1104 58-59 -0.6710732996343509 1104 60-61 -0.6023049865810677 1104 62-63 -0.660664395853999 1104 64-65 -0.6429372452804571 1104 66-67 -0.6649493401306117 1104 68-69 -0.5863381232491705 1104 70-71 -0.6483860052912078 1104 72-73 -0.6180563809715238 1104 74-75 -0.6833953190250952 1104 76-77 -0.6799655819387525 1104 78-79 -0.6815334235766954 1104 80-81 -0.7751686050798945 1104 82-83 -0.7484776113388847 1104 84-85 -0.8259360188792044 1104 86-87 -0.9097952235317521 1104 88-89 -0.7996044731675163 1104 90-91 -0.7737879151082012 1104 92-93 -0.7791288839116213 1104 94-95 -0.8398724152462123 1104 96-97 -0.9067723439250912 1104 98-99 -0.9062655599143099 1104 100 -1.0127181158188883 1105 1 -0.37908697114730217 1105 2 -0.3983183641785857 1105 3 -0.3726538443500971 1105 4 -0.22320748202481155 1105 5 -0.2289707275547883 1105 6 -0.23769006001908366 1105 7 -0.24787654331001363 1105 8 -0.23720930373349347 1105 9 -0.3959653878528826 1105 10-11 -0.4520584394461409 1105 12-13 -0.43717352868381454 1105 14-15 -0.4818416533384351 1105 16-17 -0.5633268516154644 1105 18-19 -0.5488032084874774 1105 20-21 -0.5650942731069577 1105 22-23 -0.5726353430738698 1105 24-25 -0.5786379631978207 1105 26-27 -0.6215505301351243 1105 28-29 -0.6400068182024938 1105 30-31 -0.644868314089166 1105 32-33 -0.6012556823790831 1105 34-35 -0.7271430095587377 1105 36-37 -0.7287790149847595 1105 38-39 -0.6920988741129364 1105 40-41 -0.7630885535572176 1105 42-43 -0.8071288302495532 1105 44-45 -0.8035173731739746 1105 46-47 -0.7649370161897124 1105 48-49 -0.8313028348548528 1105 50-51 -0.7885066102430685 1105 52-53 -0.6487874296425531 1105 54-55 -0.591175244017677 1105 56-57 -0.5876166230844433 1105 58-59 -0.5432249563886131 1105 60-61 -0.5188059137036092 1105 62-63 -0.5745850842567748 1105 64-65 -0.6145435473939571 1105 66-67 -0.7070016326202548 1105 68-69 -0.6252721718872465 1105 70-71 -0.5979137473627745 1105 72-73 -0.6277125762540621 1105 74-75 -0.6883035762980967 1105 76-77 -0.6697402910081678 1105 78-79 -0.5825856109502681 1105 80-81 -0.6305901677374486 1105 82-83 -0.6720553814937347 1105 84-85 -0.71948244724463 1105 86-87 -0.6825234108858709 1105 88-89 -0.7715055424256612 1105 90-91 -0.7907760928868868 1105 92-93 -0.7462848490146818 1105 94-95 -0.8284279866015751 1105 96-97 -0.8520541888002278 1105 98-99 -0.7896843264831439 1105 100 -0.8249456167714513 1106 1 -0.4475695539812463 1106 2 -0.5030052692003046 1106 3 -0.4095223498813745 1106 4 -0.37687486715815055 1106 5 -0.2949780772328978 1106 6 -0.3302583825927101 1106 7 -0.4097066830670357 1106 8 -0.36682349441976214 1106 9 -0.48851036674414416 1106 10-11 -0.53375583146277 1106 12-13 -0.4840608277320797 1106 14-15 -0.6052766539664916 1106 16-17 -0.6351764827296975 1106 18-19 -0.6450915135866921 1106 20-21 -0.6608956339324763 1106 22-23 -0.6078974265481634 1106 24-25 -0.6649163984893178 1106 26-27 -0.6760883505441484 1106 28-29 -0.7230895774850339 1106 30-31 -0.7400357519001801 1106 32-33 -0.7397517119151402 1106 34-35 -0.7483481512456223 1106 36-37 -0.8119115185428214 1106 38-39 -0.7790946953467781 1106 40-41 -0.8140128892719858 1106 42-43 -0.8186657350674835 1106 44-45 -0.8077515960251205 1106 46-47 -0.7837692849152589 1106 48-49 -0.8530042459372282 1106 50-51 -0.8375782727541861 1106 52-53 -0.6676602410333814 1106 54-55 -0.7613060081626202 1106 56-57 -0.6547759340826929 1106 58-59 -0.5910143220197241 1106 60-61 -0.7481242448304073 1106 62-63 -0.6474167248026035 1106 64-65 -0.7019579492602688 1106 66-67 -0.7228171009346553 1106 68-69 -0.6944562076739729 1106 70-71 -0.654907224347447 1106 72-73 -0.5888328395707916 1106 74-75 -0.7353148500615632 1106 76-77 -0.7383125741030554 1106 78-79 -0.674012779464114 1106 80-81 -0.7602804724144967 1106 82-83 -0.7201284222702071 1106 84-85 -0.7176696682706414 1106 86-87 -0.7345767965288168 1106 88-89 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0.7924350056379552 2311 16-17 0.8331718692437775 2311 18-19 0.740359216033589 2311 20-21 0.7833455262390032 2311 22-23 0.8800425950499644 2311 24-25 0.829715691481006 2311 26-27 0.9203570046864087 2311 28-29 0.9399746372127069 2311 30-31 0.9395593020698598 2311 32-33 0.9546091444467137 2311 34-35 0.9847319199169888 2311 36-37 0.9316746512451886 2311 38-39 0.9965393558980296 2311 40-41 0.9845744332539894 2311 42-43 1.0266955507686362 2311 44-45 1.0732856180372252 2311 46-47 1.0680041788102912 2311 48-49 1.1061986140452262 2311 50-51 1.0856213857398984 2311 52-53 0.9954287099050774 2311 54-55 0.9450475835524941 2311 56-57 0.9038880289954818 2311 58-59 0.8763650561652838 2311 60-61 0.8699325985647945 2311 62-63 0.9094160274963698 2311 64-65 0.88602088895869 2311 66-67 0.8745441755706977 2311 68-69 0.9130615068438246 2311 70-71 0.9026457785965363 2311 72-73 0.8660333864506029 2311 74-75 0.8949926367998842 2311 76-77 0.9408214656726912 2311 78-79 0.9440347169691421 2311 80-81 0.9826575657485854 2311 82-83 0.962612556664169 2311 84-85 1.0322267537835934 2311 86-87 1.0271834797187793 2311 88-89 1.0464862857445105 2311 90-91 1.0976624594864006 2311 92-93 1.1403413673682472 2311 94-95 1.2037564098920406 2311 96-97 1.1155084292371136 2311 98-99 1.2009797881819537 2311 100 1.2642097227535203 2312 1 0.5344974737525341 2312 2 0.5137608227001849 2312 3 0.5352678222061478 2312 4 0.44897962067808095 2312 5 0.4302308194003288 2312 6 0.4632279937156838 2312 7 0.45934244222981135 2312 8 0.47677468202206086 2312 9 0.5801426310066873 2312 10-11 0.5905271416560609 2312 12-13 0.6138996669496635 2312 14-15 0.8180621371412897 2312 16-17 0.7503729677967854 2312 18-19 0.7250063699828644 2312 20-21 0.7632670885162867 2312 22-23 0.7846359887085939 2312 24-25 0.7740872165226094 2312 26-27 0.8480769742133134 2312 28-29 0.8505665677410263 2312 30-31 0.9108477785264171 2312 32-33 0.8670827599336093 2312 34-35 0.8795300378137583 2312 36-37 0.8511131190291579 2312 38-39 0.9436220378743698 2312 40-41 0.9376370794794155 2312 42-43 0.9811810406104087 2312 44-45 0.939460340486221 2312 46-47 0.9892780706616122 2312 48-49 0.9618265707181592 2312 50-51 0.9860106711722452 2312 52-53 0.8551861430013403 2312 54-55 0.822141926430092 2312 56-57 0.8000770672543638 2312 58-59 0.7474669711587865 2312 60-61 0.7818737561720823 2312 62-63 0.8139408157250116 2312 64-65 0.7904969444396599 2312 66-67 0.8186137971532688 2312 68-69 0.8265185103075154 2312 70-71 0.8588265402017257 2312 72-73 0.8704785017674723 2312 74-75 0.7845305833051981 2312 76-77 0.8410738511293872 2312 78-79 0.8506622810843112 2312 80-81 0.9050817361061654 2312 82-83 0.8990744541340518 2312 84-85 0.904724658945046 2312 86-87 0.9377250795974952 2312 88-89 1.0050898775567205 2312 90-91 1.0603230047147107 2312 92-93 1.1101237984335413 2312 94-95 1.1016825979888658 2312 96-97 1.1150864939943084 2312 98-99 1.1967286190264659 2312 100 1.2459926407636637 2313 1 0.4701699822373051 2313 2 0.4718343133369345 2313 3 0.4909805179296285 2313 4 0.4106710730657426 2313 5 0.3931269069537038 2313 6 0.42502268731855253 2313 7 0.43921284751259293 2313 8 0.4610197251461585 2313 9 0.5633817019575673 2313 10-11 0.5815738449033105 2313 12-13 0.6019419752310853 2313 14-15 0.7398895131913363 2313 16-17 0.7326319650115067 2313 18-19 0.7207040608803439 2313 20-21 0.7313702803598687 2313 22-23 0.6870008220366159 2313 24-25 0.7074888408878692 2313 26-27 0.7730894120465521 2313 28-29 0.8124780602156889 2313 30-31 0.863289033374528 2313 32-33 0.8448656025305965 2313 34-35 0.894925784747997 2313 36-37 0.9200550664263218 2313 38-39 0.9185294722518904 2313 40-41 0.9599668584499383 2313 42-43 0.7878020874332634 2313 44-45 0.9534687700526518 2313 46-47 1.0120982528372693 2313 48-49 0.9878381878799303 2313 50-51 0.9519200498417462 2313 52-53 0.8591920227769165 2313 54-55 0.823370620914659 2313 56-57 0.8328081236381664 2313 58-59 0.8710514840641679 2313 60-61 0.9321329520625383 2313 62-63 0.8843554147251638 2313 64-65 0.8693179722359119 2313 66-67 0.8382964861469375 2313 68-69 0.8608696217070317 2313 70-71 0.8795566601048463 2313 72-73 0.8873548492878953 2313 74-75 0.8979059811652945 2313 76-77 0.9433976967562998 2313 78-79 0.9121705572988645 2313 80-81 0.9253346897830887 2313 82-83 0.9048633781215472 2313 84-85 0.921477367178273 2313 86-87 0.9754231355323242 2313 88-89 0.9832822573738902 2313 90-91 1.076939960115432 2313 92-93 1.0887416537874302 2313 94-95 1.0698833246843549 2313 96-97 1.146262297732921 2313 98-99 1.1343381415948635 2313 100 1.2636586835544321 2314 1 0.3838873189052485 2314 2 0.2860978846531097 2314 3 0.3340147693260036 2314 4 0.28425061222890236 2314 5 0.26674270091979224 2314 6 0.3277806416177924 2314 7 0.34792388646981465 2314 8 0.37163534098546336 2314 9 0.444900188361288 2314 10-11 0.4773623536279743 2314 12-13 0.49604801088251804 2314 14-15 0.5704768547217043 2314 16-17 0.5786644421823013 2314 18-19 0.6004816634667449 2314 20-21 0.6160552564853958 2314 22-23 0.6636432872527749 2314 24-25 0.7069123283022734 2314 26-27 0.7146846398922548 2314 28-29 0.6837860165729595 2314 30-31 0.7080600085714508 2314 32-33 0.7752025389903849 2314 34-35 0.7693062904582888 2314 36-37 0.7619076491385286 2314 38-39 0.7938069700223096 2314 40-41 0.7841809022704709 2314 42-43 0.6827873493053147 2314 44-45 0.8399909102453265 2314 46-47 0.7357826872854787 2314 48-49 0.6116847919023201 2314 50-51 0.7417415854099829 2314 52-53 0.7435042655086406 2314 54-55 0.7325624776914879 2314 56-57 0.7323379151966378 2314 58-59 0.7138631817535739 2314 60-61 0.5230157906979045 2314 62-63 0.6544140274509047 2314 64-65 0.6895018719298847 2314 66-67 0.6663785361565218 2314 68-69 0.7181034606910615 2314 70-71 0.7431717573246246 2314 72-73 0.7321432554686567 2314 74-75 0.7526520130249352 2314 76-77 0.769918475010833 2314 78-79 0.8000591489847437 2314 80-81 0.8579239517662067 2314 82-83 0.8429890184508437 2314 84-85 0.8463084382444954 2314 86-87 0.8786196870760499 2314 88-89 0.7578695054405031 2314 90-91 0.7817651424057246 2314 92-93 0.9257856453833817 2314 94-95 0.9838404529361569 2314 96-97 0.9909052964337413 2314 98-99 0.9361077034379015 2314 100 1.020475944416802 2315 1 0.3016735231598737 2315 2 0.2764353965508448 2315 3 0.28758104074699276 2315 4 0.23495227278868214 2315 5 0.2532365133998269 2315 6 0.23889143343061647 2315 7 0.27235001283654015 2315 8 0.2251694443681984 2315 9 0.14264965610183822 2315 10-11 0.29986309750675133 2315 12-13 0.2385906226227945 2315 14-15 0.40456547162877854 2315 16-17 0.2854440553536364 2315 18-19 0.43065810948821337 2315 20-21 0.31655801841242237 2315 22-23 0.20625403806926812 2315 24-25 0.42028042211227756 2315 26-27 0.5456585171322672 2315 28-29 0.42802142845660995 2315 30-31 0.44991727782682744 2315 32-33 0.5015602722653725 2315 34-35 0.3624593769105289 2315 36-37 0.5784140604824373 2315 38-39 0.6753738137096192 2315 40-41 0.7202979004211798 2315 42-43 0.7083226821634483 2315 44-45 0.7325991819399817 2315 46-47 0.7358883191684455 2315 48-49 0.6037916698396373 2315 50-51 0.5871946597782767 2315 52-53 0.3261720075206469 2315 54-55 0.5131433070474287 2315 56-57 0.47319714591240825 2315 58-59 0.48333317703661294 2315 60-61 0.5666868973995989 2315 62-63 0.5486311785156062 2315 64-65 0.5902056040456571 2315 66-67 0.4489938606500843 2315 68-69 0.5343350271848806 2315 70-71 0.5290355420909165 2315 72-73 0.5476890162422379 2315 74-75 0.3460739852514081 2315 76-77 0.5320609179644009 2315 78-79 0.5973977326546773 2315 80-81 0.6373356726105364 2315 82-83 0.5506283801863177 2315 84-85 0.6060846770388437 2315 86-87 0.6282125397706295 2315 88-89 0.555343424944649 2315 90-91 0.6703578360805444 2315 92-93 0.809450845671762 2315 94-95 0.7102604060334947 2315 96-97 0.7408987806627643 2315 98-99 0.8788649061117333 2315 100 0.9751986300752975 2316 1 0.054197893131885166 2316 2 0.08965517107873566 2316 3 0.056313892302206625 2316 4 -0.03962132097991855 2316 5 -0.03337905289309617 2316 6 -0.18140991824006392 2316 7 -0.1261826957848342 2316 8 -0.0914565006289294 2316 9 -0.003422130576474558 2316 10-11 -0.030775972568299892 2316 12-13 0.026680858832719423 2316 14-15 -0.12177966721042566 2316 16-17 0.0254281198988906 2316 18-19 0.09937569795919643 2316 20-21 0.18535441694558585 2316 22-23 0.09035832140089184 2316 24-25 0.05423942337481691 2316 26-27 -0.05644589317586224 2316 28-29 0.01305785640996504 2316 30-31 -0.5516603553617045 2316 32-33 -0.37243209106004116 2316 34-35 -0.39648815069265453 2316 36-37 -0.1928798083753236 2316 38-39 -0.13780358168596507 2316 40-41 0.10702812125234118 2316 42-43 0.17962688113581748 2316 44-45 0.0065118165059985245 2316 46-47 -0.13727926402605561 2316 48-49 -0.05959896306245582 2316 50-51 0.14424763502907467 2316 52-53 -0.224272674912676 2316 54-55 -0.1475532503045116 2316 56-57 -0.16204630989442137 2316 58-59 -0.5233640773667787 2316 60-61 -0.09258831389231403 2316 62-63 -0.11033823612351767 2316 64-65 -0.4267131141461604 2316 66-67 -0.676795746329482 2316 68-69 -0.6809677058299144 2316 70-71 -0.43125326815613363 2316 72-73 -0.6325635316904723 2316 74-75 -0.43482896169273744 2316 76-77 -0.11222555122977695 2316 78-79 -0.04605306397406039 2316 80-81 -0.132831456753987 2316 82-83 0.137310482843219 2316 84-85 0.15251567834913615 2316 86-87 0.22157247708426553 2316 88-89 0.25465015527376167 2316 90-91 -0.32820632947345274 2316 92-93 -0.39206310297557323 2316 94-95 0.013466127551012619 2316 96-97 0.22275639318173646 2316 98-99 0.2712783310871778 2316 100 0.21936857515828123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 336.0 1 979.0 2 540.0 3 544.0 4 508.0 5 466.0 6 533.0 7 1199.0 8 2982.0 9 5473.0 10 8741.0 11 14356.0 12 21043.0 13 26956.0 14 31578.0 15 36927.0 16 44561.0 17 55000.0 18 66853.0 19 80321.0 20 97172.0 21 117653.0 22 129819.0 23 133730.0 24 135164.0 25 143067.0 26 159128.0 27 183691.0 28 215651.0 29 259054.0 30 314859.0 31 391444.0 32 493460.0 33 646488.0 34 887987.0 35 1238786.0 36 1832691.0 37 2589883.0 38 2302674.0 39 530650.0 40 272.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.70454259122937 18.903681690050576 12.696591615277978 23.69518410344208 2 15.821815822232843 19.879642950458262 40.55192308479691 23.74661814251198 3 19.206360496996396 25.82183854366581 28.831455783475484 26.14034517586231 4 12.65262235468473 16.056470157604597 38.064064497498805 33.22684299021187 5 13.578531973164587 37.55774445997486 34.374681122859634 14.489042444000914 6 31.267272529986535 36.65617307112933 18.056859698713126 14.019694700171007 7 28.322297337949088 31.783712171096752 22.05681573810282 17.837174752851343 8 26.475651325047135 35.013280627044416 19.99889075547735 18.512177292431105 9 26.894136896414356 15.727468184124845 20.44351527709022 36.934879642370575 10-11 25.385332078738816 25.913008836435814 27.820680216216775 20.8809788686086 12-13 25.771106063920246 23.56069507985769 28.17545515068673 22.492743705535332 14-15 23.163004028427913 25.002232164926475 25.712123115951467 26.122640690694144 16-17 22.501262574554975 27.923686436602242 26.553991461203836 23.02105952763895 18-19 22.252640950116962 27.2305416386487 28.558761968802976 21.95805544243136 20-21 23.153568455480062 26.417131332395034 28.381078737786375 22.048221474338526 22-23 23.203892312249874 26.21137390549367 28.081808209501734 22.502925572754723 24-25 22.760990431513893 26.366792738025957 28.46357745036639 22.40863938009376 26-27 22.65603996856672 26.588931989634858 28.145988697191125 22.609039344607293 28-29 22.46804550541451 26.623184055183557 28.37943235042793 22.529338088974008 30-31 22.89459703519944 26.506760402713603 28.234182705120407 22.364459856966548 32-33 22.781661794328887 26.781710743128784 27.937474500308333 22.499152962233993 34-35 22.89383380384763 26.664779153795624 28.201795054291946 22.239591988064795 36-37 22.89633057615289 26.44984206960757 28.000016366236363 22.65381098800317 38-39 22.842079544080722 26.63782530123609 28.15408833136695 22.36600682331624 40-41 22.800846088922697 26.2627820335878 28.412063871567263 22.524308005922244 42-43 22.677542674546274 26.33375706553458 28.106850688374426 22.88184957154472 44-45 22.581159389592013 26.725100611236304 28.027001994889233 22.666738004282454 46-47 22.702969055584482 26.571203826162833 27.708400363418473 23.017426754834208 48-49 22.894454245899304 26.610101157076905 27.63096688826038 22.86447770876341 50-51 22.795807265907335 26.65777992791337 27.50977241931251 23.036640386866782 52-53 22.63820323123896 26.89652849128741 27.431813236670667 23.03345504080296 54-55 22.58898538969158 26.822340516788017 27.49672335773083 23.09195073578957 56-57 22.825838443947337 26.7811759452915 27.276519083233637 23.11646652752752 58-59 22.57950945080527 26.713493171913495 27.651420140449208 23.055577236832033 60-61 22.660184936702958 26.993834975949255 27.3408323564856 23.005147730862184 62-63 22.69979318627944 26.794755626601013 27.319765870287462 23.18568531683209 64-65 22.440366449666293 26.66730422698703 27.448047864310592 23.44428145903608 66-67 22.638344838665496 26.58621553917948 27.28736416531229 23.48807545684274 68-69 22.417681209821232 26.658397770990266 27.442566142418833 23.48135487676967 70-71 22.55426396051991 26.402182816282092 27.435124558627 23.608428664570997 72-73 22.74680939699966 26.563513505218133 27.29941941911901 23.390257678663197 74-75 22.728784657962752 26.70971831468863 27.206499576309795 23.354997451038823 76-77 22.633936484151803 26.609839651244886 27.215909189937303 23.540314674666007 78-79 22.65417880058828 26.556839045276043 27.193154702403966 23.59582745173171 80-81 22.556585599903723 26.843797529808427 27.15022778688518 23.44938908340267 82-83 22.966533627061665 26.44569649374246 27.44449002112028 23.143279858075598 84-85 22.989484611326432 26.526710810537068 27.05533915920466 23.428465418931836 86-87 22.558137702039843 26.731100510521742 27.35658967716669 23.354172110271726 88-89 22.884506986747244 26.308343758839893 27.256099513425834 23.55104974098703 90-91 22.957828397740442 26.330735756579536 27.246206104974863 23.46522974070516 92-93 23.096900873786797 26.37369800400849 27.138802438444465 23.390598683760246 94-95 22.7758479301092 26.128013416772284 27.390485997956954 23.70565265516156 96-97 22.935655346991567 26.092612588249185 27.281491504293818 23.690240560465426 98-99 22.84613317868896 26.245680426223593 27.120000779235436 23.788185615852015 100 23.274077734354915 25.98749852312218 26.69778006494774 24.04064367757516 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3041.0 1 2180.0 2 3303.5 3 4417.0 4 2766.0 5 2165.0 6 2986.5 7 5148.5 8 7936.0 9 9103.0 10 8080.0 11 7208.0 12 7737.0 13 8573.5 14 9411.5 15 10444.0 16 11337.0 17 12239.0 18 13293.5 19 14407.5 20 15540.5 21 17134.0 22 19622.0 23 22588.0 24 26570.0 25 32287.5 26 40320.5 27 50757.5 28 63369.0 29 82448.5 30 108338.5 31 134553.0 32 168693.0 33 208243.0 34 245451.5 35 282860.5 36 327414.0 37 376020.5 38 399376.0 39 402517.5 40 397820.0 41 391271.0 42 396725.5 43 416712.5 44 442585.5 45 475706.5 46 503786.0 47 519650.5 48 536788.5 49 545407.0 50 539779.0 51 532609.0 52 520685.0 53 502896.0 54 484377.0 55 448134.5 56 405452.0 57 356491.5 58 307953.0 59 273797.5 60 232382.0 61 187140.0 62 150355.0 63 118844.0 64 89422.0 65 63758.5 66 46090.5 67 34990.0 68 26783.0 69 20192.0 70 15668.0 71 12317.5 72 9688.0 73 7544.5 74 5896.5 75 4488.5 76 3273.5 77 2214.5 78 1462.0 79 1010.0 80 719.0 81 485.0 82 358.5 83 266.5 84 201.0 85 152.5 86 113.0 87 93.5 88 72.5 89 58.5 90 44.0 91 29.5 92 22.5 93 27.0 94 23.5 95 13.0 96 11.0 97 10.0 98 5.5 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025221122212696765 2 0.10952632081615854 3 0.01695041186547008 4 0.01768508119118527 5 0.019707315314545643 6 0.0451632287550483 7 0.046208428414313205 8 0.03822552666891309 9 0.03260568502272059 10-11 0.036339622935891616 12-13 0.03543075366696561 14-15 0.03142794192840398 16-17 0.03778245290031166 18-19 0.03668802282231325 20-21 0.04754143667540468 22-23 0.04718924983369586 24-25 0.06594982632644357 26-27 0.05793662893874592 28-29 0.05058993568159401 30-31 0.04882142756247548 32-33 0.04359164231086374 34-35 0.04512535920217638 36-37 0.04026290861342222 38-39 0.045220033084356175 40-41 0.05458138655429407 42-43 0.055929542636534324 44-45 0.05869401999618427 46-47 0.06030726294852793 48-49 0.059826319627054585 50-51 0.05416860842799017 52-53 0.06412072692272998 54-55 0.05137383542604269 56-57 0.055410729762189054 58-59 0.05293406100436568 60-61 0.04872675368029569 62-63 0.055183512444957554 64-65 0.05363843468778333 66-67 0.05151395277166879 68-69 0.05743675084083662 70-71 0.05760337687347305 72-73 0.06048524984702595 74-75 0.05049147484412703 76-77 0.05147229626350967 78-79 0.061477432132270166 80-81 0.06218180581568783 82-83 0.05224483514209678 84-85 0.05796692458104346 86-87 0.0553425645670196 88-89 0.06374581834929799 90-91 0.06912708181239742 92-93 0.07366385424645308 94-95 0.07710240964722315 96-97 0.07280800235154775 98-99 0.08180202115862806 100 0.12585567201452919 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.3203219E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.77012869913461 #Duplication Level Percentage of deduplicated Percentage of total 1 70.08442062472471 34.88110634296832 2 15.589769955067712 15.51809714107244 3 6.027593531235582 8.999823173869986 4 2.875301488477504 5.724165005213548 5 1.6296439625053762 4.055379487383013 6 0.9579985288374723 2.8607826044293567 7 0.6340364377710066 2.2089252575462694 8 0.4194222188755459 1.6699758250170023 9 0.3064441813906147 1.372658971222066 >10 1.3529984735137932 11.44669911888706 >50 0.07040670500453751 2.397403308514825 >100 0.04549416744491711 4.5073181729398195 >500 0.004495261029098215 1.5110228990772019 >1k 0.0018445651730478966 1.556224172711975 >5k 5.1959579695163733E-5 0.1776476721666883 >10k+ 7.793936954274561E-5 1.1127708469805078 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39058 0.2958217992142674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28691 0.21730306828963453 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18311 0.1386858765275347 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.5147821148766828E-5 2 0.0 0.0 0.0 0.0 1.5147821148766828E-5 3 0.0 0.0 0.0 7.573910574383414E-6 1.5147821148766828E-5 4 0.0 0.0 0.0 1.5147821148766828E-5 1.5147821148766828E-5 5 0.0 1.5147821148766828E-5 0.0 1.5147821148766828E-5 1.5147821148766828E-5 6 0.0 1.5147821148766828E-5 0.0 1.5147821148766828E-5 3.0295642297533655E-5 7 0.0 1.5147821148766828E-5 0.0 1.5147821148766828E-5 6.059128459506731E-5 8 0.0 1.5147821148766828E-5 0.0 1.5147821148766828E-5 6.059128459506731E-5 9 0.0 1.5147821148766828E-5 0.0 3.786955287191707E-5 9.846083746698438E-5 10-11 0.0 1.5147821148766828E-5 0.0 4.544346344630048E-5 9.846083746698438E-5 12-13 0.0 3.0295642297533655E-5 0.0 4.544346344630048E-5 1.5905212206205168E-4 14-15 0.0 3.0295642297533655E-5 0.0 6.059128459506731E-5 2.0070863022116046E-4 16-17 0.0 3.786955287191707E-5 0.0 8.709997160540926E-5 2.6129991481622775E-4 18-19 0.0 5.3017374020683895E-5 0.0 1.5147821148766828E-4 2.84021646539378E-4 20-21 0.0 6.816519516945072E-5 0.0 3.0295642297533656E-4 3.370390205600619E-4 22-23 0.0 6.816519516945072E-5 0.0 9.240170900747765E-4 3.5976075228321216E-4 24-25 0.0 6.816519516945072E-5 0.0 0.0019692167493396877 4.014172604423209E-4 26-27 0.0 6.816519516945072E-5 0.0 0.003885416124658691 4.279259474526629E-4 28-29 0.0 8.331301631821755E-5 0.0 0.008895557969613319 4.506476791758131E-4 30-31 0.0 8.331301631821755E-5 0.0 0.025762656818765182 4.582215897501965E-4 32-33 0.0 8.331301631821755E-5 0.0 0.05997401088325506 4.6200854503738824E-4 34-35 0.0 9.088692689260096E-5 0.0 0.11088962471954755 4.923041873349219E-4 36-37 0.0 1.0224779275417608E-4 0.0 0.191790350519824 4.923041873349219E-4 38-39 0.0 1.098217033285595E-4 0.0 0.3105265465944328 4.923041873349219E-4 40-41 0.0 1.136086586157512E-4 0.0 0.4415892821288505 4.960911426221136E-4 42-43 0.0 1.136086586157512E-4 0.0 0.5716143919145777 5.112389637708804E-4 44-45 0.0 1.2118256919013462E-4 0.0 0.7274135193849318 5.225998296324555E-4 46-47 0.0 1.2118256919013462E-4 0.0 0.9114822680741719 5.415346060684141E-4 48-49 0.0 1.2118256919013462E-4 0.0 1.1079495083736777 5.528954719299892E-4 50-51 0.0 1.2118256919013462E-4 0.0 1.3157776145347584 5.68043293078756E-4 52-53 0.0 1.2118256919013462E-4 0.0 1.5191068178146556 6.059128459506731E-4 54-55 0.0 1.3254343505170974E-4 0.0 1.7352775864734198 6.210606670994399E-4 56-57 0.0 1.3633039033890144E-4 0.0 1.9849969920214154 6.248476223866316E-4 58-59 0.0 1.8177385378520192E-4 0.0 2.2639403315206694 6.362084882482067E-4 60-61 0.0 1.8556080907239364E-4 0.0 2.5529456112179916 6.513563093969736E-4 62-63 0.0 1.8934776435958534E-4 0.0 2.844673711766805 6.930128175560823E-4 64-65 0.0 1.9313471964677704E-4 0.0 3.144210514117807 6.967997728432741E-4 66-67 0.0 2.0449558550835216E-4 0.0 3.4729030852248988 6.967997728432741E-4 68-69 0.0 2.0449558550835216E-4 0.0 3.8352806236115597 7.081606387048492E-4 70-71 0.0 2.0828254079554388E-4 0.0 4.22353821443089 7.23308459853616E-4 72-73 0.0 2.1206949608273558E-4 0.0 4.610966462042325 7.914736550230667E-4 74-75 0.0 2.1206949608273558E-4 0.0 4.992642324572515 8.217692973206004E-4 76-77 0.0 2.1206949608273558E-4 0.0 5.405802933360418 8.407040737565589E-4 78-79 0.0 2.1206949608273558E-4 0.0 5.847793632749711 8.672127607669009E-4 80-81 0.0 2.19643406657119E-4 0.0 6.3208070698516785 8.899344924900511E-4 82-83 0.0 2.19643406657119E-4 0.0 6.802000330373978 9.69460553521077E-4 84-85 3.786955287191707E-6 2.272173172315024E-4 0.0 7.268102574076822 9.732475088082686E-4 86-87 7.573910574383414E-6 2.3479122780588582E-4 0.0 7.741718137069453 9.921822852442272E-4 88 7.573910574383414E-6 2.3479122780588582E-4 0.0 8.11983804858497 0.0010376257486905277 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20490 0.0 47.357693 1 GTATCAA 37495 0.0 41.51038 1 ATCAACG 45430 0.0 33.32725 3 CAACGCA 45575 0.0 33.21078 5 TATCAAC 47825 0.0 32.151028 2 TCAACGC 48595 0.0 31.349566 4 AACGCAG 49335 0.0 30.975172 6 TACCTGG 9725 0.0 29.745443 2 GTACATG 55750 0.0 28.238663 1 GTACTAG 5295 0.0 27.804281 1 ACGCAGA 55600 0.0 27.333488 7 TACATGG 56760 0.0 27.147911 2 CGCAGAG 57165 0.0 26.50919 8 ACATGGG 57080 0.0 25.998293 3 CATGGGG 35740 0.0 23.915983 4 GCAGAGT 62235 0.0 23.837635 9 TAGTACT 7325 0.0 23.15846 4 GTACCTG 13065 0.0 22.96913 1 ACCTGGG 12280 0.0 22.921612 3 CTAGTAC 7310 0.0 22.177797 3 >>END_MODULE