FastQCFastQC Report
Mon 6 Jun 2016
ERR1211356_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211356_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13203219
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341430.2585960287411729No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267910.20291263819830602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180680.1368454162579595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA209700.047.0427321
GTATCAA368300.039.5019231
ATCAACG456200.031.2157973
CAACGCA459450.031.0695085
TATCAAC484350.029.6313692
TCAACGC488200.029.342924
AACGCAG494000.029.257386
TACCTGG100400.028.8325462
GTACATG556200.026.308941
GTACTAG53550.025.6783071
ACGCAGA561000.025.5039627
TACATGG569400.025.3207552
CGCAGAG579000.024.6543798
ACATGGG563500.024.5556763
ACCTGGG123650.023.2614823
TAGTACT73750.022.077094
GCAGAGT643600.021.959929
CATGGGG358300.021.8435654
GTACCTG137350.021.6288431
TCTAACG27800.021.433372
Produced by FastQC (version 0.11.5)