##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211356_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13203219 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04899502159284 33.0 31.0 34.0 30.0 34.0 2 32.18180884525206 33.0 31.0 34.0 30.0 34.0 3 32.251187305156414 34.0 31.0 34.0 30.0 34.0 4 35.90308764854995 37.0 35.0 37.0 35.0 37.0 5 35.803833368211194 37.0 35.0 37.0 35.0 37.0 6 35.81912880487705 37.0 35.0 37.0 35.0 37.0 7 35.7320545845676 37.0 35.0 37.0 33.0 37.0 8 35.715105308788715 37.0 35.0 37.0 33.0 37.0 9 37.32155673552033 39.0 37.0 39.0 34.0 39.0 10-11 37.265326773720865 39.0 37.0 39.0 34.0 39.0 12-13 37.19210909854635 39.0 37.0 39.0 33.0 39.0 14-15 38.411979343825166 40.0 38.0 41.0 33.5 41.0 16-17 38.3975449093134 40.0 38.0 41.0 33.0 41.0 18-19 38.39868413907245 40.0 38.0 41.0 33.5 41.0 20-21 38.36530845243118 40.0 38.0 41.0 34.0 41.0 22-23 38.24905604459033 40.0 38.0 41.0 33.5 41.0 24-25 38.15739888886188 40.0 37.5 41.0 33.0 41.0 26-27 38.056401170048005 40.0 37.0 41.0 33.0 41.0 28-29 37.95324424293803 40.0 37.0 41.0 33.0 41.0 30-31 37.96433619710466 40.0 37.0 41.0 33.0 41.0 32-33 37.90581357470478 40.0 37.0 41.0 33.0 41.0 34-35 37.774965180839615 40.0 37.0 41.0 33.0 41.0 36-37 37.625354127656294 40.0 37.0 41.0 32.5 41.0 38-39 37.505898902381304 40.0 36.5 41.0 32.0 41.0 40-41 37.39586387986142 40.0 36.0 41.0 32.0 41.0 42-43 37.29069081562609 39.5 36.0 41.0 31.5 41.0 44-45 37.12914543794207 39.0 36.0 41.0 31.0 41.0 46-47 36.890525636210384 39.0 35.5 41.0 30.5 41.0 48-49 35.328955196456256 39.0 35.0 40.0 24.0 41.0 50-51 36.06587726826314 38.5 35.0 40.0 28.5 41.0 52-53 36.16057890882519 38.5 35.0 40.0 29.0 41.0 54-55 35.98584091500717 38.0 35.0 40.0 29.0 41.0 56-57 35.7629194819839 38.0 34.5 40.0 28.0 41.0 58-59 35.51622066558163 38.0 34.0 40.0 28.0 41.0 60-61 35.25704883028904 37.0 34.0 40.0 28.0 41.0 62-63 34.93182594335518 37.0 34.0 40.0 27.0 41.0 64-65 34.59179965885592 36.0 33.0 39.0 26.5 41.0 66-67 34.250167856793105 36.0 33.0 39.0 26.0 40.0 68-69 33.8896813345291 35.0 33.0 38.5 26.0 40.0 70-71 33.53672131015929 35.0 32.0 38.0 26.0 40.0 72-73 33.16298646564903 35.0 32.0 37.0 25.5 39.5 74-75 32.804553154802626 35.0 32.0 37.0 25.0 39.0 76-77 31.880650014212442 34.0 30.5 36.0 24.5 38.0 78-79 32.33154759456765 34.5 31.0 36.0 25.0 38.0 80-81 32.210171322614585 34.5 31.0 35.5 25.0 37.0 82-83 31.94516590991939 34.0 31.0 35.0 24.5 37.0 84-85 31.666454900127007 34.0 31.0 35.0 24.0 36.0 86-87 31.406507723608918 34.0 31.0 35.0 23.5 36.0 88-89 31.17657349317617 34.0 31.0 35.0 23.0 36.0 90-91 30.954493332269955 34.0 30.0 35.0 22.0 35.0 92-93 30.754688345319426 34.0 30.0 35.0 20.0 35.0 94-95 30.55429823590747 34.0 30.0 35.0 20.0 35.0 96-97 30.359389062621773 34.0 29.0 35.0 20.0 35.0 98-99 30.131672245987893 34.0 29.0 35.0 19.0 35.0 100 27.65607493142392 31.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.29228680995505485 1101 2 -0.29467616092883375 1101 3 -0.3558192072816979 1101 4 -0.21951234351625004 1101 5 -0.21906756623835122 1101 6 -0.2678895029098456 1101 7 -0.3125616342667925 1101 8 -0.2838440380149905 1101 9 -0.39185531596437073 1101 10-11 -0.36932302625002933 1101 12-13 -0.45323856112777605 1101 14-15 -0.5869122029648963 1101 16-17 -0.5552793295680729 1101 18-19 -0.568095347467306 1101 20-21 -0.5632015990090551 1101 22-23 -0.6593505695275468 1101 24-25 -0.6280549803682334 1101 26-27 -0.6948584980362895 1101 28-29 -0.7077915031750379 1101 30-31 -0.6639140065000149 1101 32-33 -0.5761288863307499 1101 34-35 -0.6612880495346545 1101 36-37 -0.6697277981687222 1101 38-39 -0.6206870278809689 1101 40-41 -0.6389051937365195 1101 42-43 -0.5999142316413497 1101 44-45 -0.5371068543207471 1101 46-47 -0.6405276230394534 1101 48-49 0.5746380146470145 1101 50-51 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0.5013290048772703 2311 10-11 0.4072391114383933 2311 12-13 0.5122423523195536 2311 14-15 0.4629912690953546 2311 16-17 0.49021904162461993 2311 18-19 0.6344838229113137 2311 20-21 0.7158641341802863 2311 22-23 0.6984199852807649 2311 24-25 0.6817753708999632 2311 26-27 0.6991255498932247 2311 28-29 0.7987739123360811 2311 30-31 0.6590052011642697 2311 32-33 0.7197307817563043 2311 34-35 0.8338378246798399 2311 36-37 0.8567654692793383 2311 38-39 0.8874396091037724 2311 40-41 0.839689296781259 2311 42-43 0.8737913709009035 2311 44-45 0.9039055065302009 2311 46-47 0.9925890212099233 2311 48-49 -5.939109035055672 2311 50-51 -0.9161520272739594 2311 52-53 0.4886267734169678 2311 54-55 0.9384941681858052 2311 56-57 1.0492254235695242 2311 58-59 1.11552887315721 2311 60-61 1.017057394942718 2311 62-63 0.827054784299321 2311 64-65 0.9359957942009274 2311 66-67 1.0086240118307188 2311 68-69 1.045117395627507 2311 70-71 1.0579877096469943 2311 72-73 0.5161037297727589 2311 74-75 0.9116919428759189 2311 76-77 0.9828328610067842 2311 78-79 0.8843718505616778 2311 80-81 0.9856620307870543 2311 82-83 0.9740632302932646 2311 84-85 1.0190738915793496 2311 86-87 1.052109977177217 2311 88-89 1.035719610797532 2311 90-91 1.078789850336463 2311 92-93 1.0860143682727887 2311 94-95 1.126552334676365 2311 96-97 1.1873315307010657 2311 98-99 1.22493960641944 2311 100 1.3536398093592439 2312 1 0.10056532814638786 2312 2 0.24267054983828018 2312 3 0.18962266780932424 2312 4 0.01671660205721537 2312 5 0.1964795095661529 2312 6 0.13601866842833488 2312 7 0.29019704292036863 2312 8 0.35176442387501083 2312 9 0.2950433100309908 2312 10-11 0.2420634742329284 2312 12-13 0.30925488792320266 2312 14-15 0.3921270036417752 2312 16-17 0.5009089402808584 2312 18-19 0.3865097321062052 2312 20-21 0.3920990701827236 2312 22-23 0.6239941073117095 2312 24-25 0.7219071451540842 2312 26-27 0.6384538790586163 2312 28-29 0.7538120762343254 2312 30-31 0.8158123738038157 2312 32-33 0.7958963879465557 2312 34-35 0.4334524133444049 2312 36-37 0.7051957539097202 2312 38-39 0.7955823016499934 2312 40-41 0.602853380255759 2312 42-43 0.6307055577498772 2312 44-45 0.796743059637727 2312 46-47 0.8767191175757105 2312 48-49 -4.595936676057974 2312 50-51 -0.6838630874214928 2312 52-53 0.6115676800384335 2312 54-55 0.878886997873316 2312 56-57 0.9565593712102753 2312 58-59 0.9840047787354038 2312 60-61 1.0002358487514158 2312 62-63 0.7554902812640378 2312 64-65 1.0023293033165217 2312 66-67 0.9841401041510025 2312 68-69 0.9169821337817794 2312 70-71 0.9897547538602822 2312 72-73 1.015514232229478 2312 74-75 0.703681127023458 2312 76-77 0.8759984383782751 2312 78-79 0.5323124828222916 2312 80-81 0.7580563849469257 2312 82-83 0.8007027589762892 2312 84-85 0.8373943276049154 2312 86-87 0.9306718505746545 2312 88-89 0.985569543675215 2312 90-91 0.8270291124224833 2312 92-93 0.9536899793465743 2312 94-95 1.111629400333971 2312 96-97 1.1671589928600987 2312 98-99 1.2047558615248342 2312 100 0.7718344528928469 2313 1 0.061952234452164134 2313 2 0.2128151119195607 2313 3 0.3053194575813478 2313 4 0.07375715046816111 2313 5 0.15583831446857488 2313 6 0.2701650715306485 2313 7 0.18224478415233847 2313 8 0.27027079842434887 2313 9 0.4690029525060666 2313 10-11 0.2723239237818902 2313 12-13 0.03624480086529047 2313 14-15 0.15228392043494665 2313 16-17 0.1045979446620251 2313 18-19 0.43518346072060154 2313 20-21 0.4108835856637256 2313 22-23 0.5204859587565878 2313 24-25 0.34126772597360144 2313 26-27 0.49171488495569804 2313 28-29 0.47058386117771533 2313 30-31 0.5958344090732766 2313 32-33 0.5538293712267475 2313 34-35 0.45437851826304865 2313 36-37 -0.2829209048662875 2313 38-39 0.2296901686424846 2313 40-41 0.4488159474979341 2313 42-43 0.5693022365708345 2313 44-45 0.5632243790090641 2313 46-47 0.47211424039902994 2313 48-49 -5.170790653682861 2313 50-51 -1.2399587155877754 2313 52-53 0.23682114811629873 2313 54-55 0.3916645540460806 2313 56-57 0.39168399873680926 2313 58-59 0.4869461297485813 2313 60-61 0.8049328275236363 2313 62-63 0.8829458767743787 2313 64-65 1.0018561742308734 2313 66-67 1.0354645245257146 2313 68-69 1.0120226933038836 2313 70-71 1.0163630056022441 2313 72-73 0.7517104948201805 2313 74-75 0.8778533872782432 2313 76-77 0.904394958617118 2313 78-79 0.8187405851634679 2313 80-81 -0.050426380421548345 2313 82-83 0.35391761447439407 2313 84-85 0.7266220252959137 2313 86-87 0.8797674860719269 2313 88-89 0.8330821052985229 2313 90-91 0.8880459299564478 2313 92-93 0.9668534296644999 2313 94-95 0.842560231404132 2313 96-97 0.9102913007481241 2313 98-99 1.0853629984468611 2313 100 1.1078543202624402 2314 1 0.02478033374210753 2314 2 0.18176182139395536 2314 3 0.22027450298157447 2314 4 0.07068373007953 2314 5 0.21551196569314612 2314 6 0.3019062090165434 2314 7 0.10414496624891001 2314 8 0.2378464937402356 2314 9 0.4082768478539833 2314 10-11 0.33277304938169294 2314 12-13 0.2553077566156716 2314 14-15 0.2940986496651874 2314 16-17 0.4953128989900577 2314 18-19 0.5220038858252636 2314 20-21 0.612977040043944 2314 22-23 0.35829292207984764 2314 24-25 0.48633176315578197 2314 26-27 0.5471014098425258 2314 28-29 0.6489776219627927 2314 30-31 0.6020287295085964 2314 32-33 0.5924688128646523 2314 34-35 0.45006200063645707 2314 36-37 0.6065614395746977 2314 38-39 0.6293374103138873 2314 40-41 0.3836078736486428 2314 42-43 0.41416503206873756 2314 44-45 0.6396252357925576 2314 46-47 0.7362469944925891 2314 48-49 -6.383119431809476 2314 50-51 -1.4663606907481253 2314 52-53 -0.7202799536689639 2314 54-55 -0.2980166141336227 2314 56-57 0.018944698733399434 2314 58-59 0.49597660170501 2314 60-61 0.714220935789676 2314 62-63 0.6057150315233102 2314 64-65 0.7869795245007225 2314 66-67 0.8701699375296741 2314 68-69 0.9073626474501069 2314 70-71 0.6455444397806218 2314 72-73 0.8060390545140734 2314 74-75 0.8353235390449356 2314 76-77 0.7823084135214415 2314 78-79 0.5620296296777525 2314 80-81 0.6398885257398561 2314 82-83 0.6712157177261524 2314 84-85 0.7028759346742426 2314 86-87 0.7664459032007045 2314 88-89 0.6255597920573699 2314 90-91 0.867204975232724 2314 92-93 0.9190089699838744 2314 94-95 0.9384688201940641 2314 96-97 0.986904160203462 2314 98-99 1.0588664382692023 2314 100 -0.2463688272771627 2315 1 0.11871703562162139 2315 2 0.22345365752017443 2315 3 0.325225169919797 2315 4 0.173707167277243 2315 5 0.26089287002494643 2315 6 0.3182638620786662 2315 7 0.34385585277467356 2315 8 0.30384830768861804 2315 9 0.46190598275066463 2315 10-11 0.2436321093505498 2315 12-13 0.4490106867735193 2315 14-15 0.2629885678214947 2315 16-17 0.4218116134753771 2315 18-19 0.48339306867671183 2315 20-21 0.387028548792145 2315 22-23 0.4237228293166453 2315 24-25 0.4801821390952483 2315 26-27 0.5560526183435144 2315 28-29 0.6394904298392632 2315 30-31 0.6406135810964813 2315 32-33 0.6493509834990547 2315 34-35 0.6945586041176384 2315 36-37 0.6129873469529983 2315 38-39 0.6709089677866515 2315 40-41 0.7169310916555816 2315 42-43 0.7640795058191969 2315 44-45 0.7321446995808927 2315 46-47 0.6457358164731346 2315 48-49 -4.844423405768236 2315 50-51 -0.8672959511448681 2315 52-53 0.3141580008030047 2315 54-55 0.3008364695790249 2315 56-57 0.6150170476600181 2315 58-59 0.7262255688466084 2315 60-61 0.6017079781012669 2315 62-63 0.3921437216457875 2315 64-65 0.5786298766819087 2315 66-67 -0.12974896987074658 2315 68-69 0.6348140997144824 2315 70-71 0.6261180434777174 2315 72-73 0.7247447890743786 2315 74-75 0.8882647369323919 2315 76-77 0.8321267102663228 2315 78-79 0.7569526486170162 2315 80-81 0.6406631730932091 2315 82-83 0.6105353016273476 2315 84-85 0.6465155519368331 2315 86-87 0.683457598259892 2315 88-89 0.6406033276880514 2315 90-91 0.7529873358204391 2315 92-93 0.747647785483597 2315 94-95 0.4747309243814968 2315 96-97 0.6144734046852101 2315 98-99 0.5450607223626527 2315 100 0.669530777853705 2316 1 0.2523890888090108 2316 2 0.2712932621901203 2316 3 0.20994083337420477 2316 4 -0.024611251599480966 2316 5 0.18686321901613212 2316 6 0.20363518734412622 2316 7 0.3013113630119406 2316 8 0.3698623657510183 2316 9 0.4916193696540745 2316 10-11 0.4546143388986792 2316 12-13 0.3805472226617397 2316 14-15 0.5192161684429379 2316 16-17 0.5146238278556652 2316 18-19 0.16764710206719258 2316 20-21 0.4781373762640442 2316 22-23 0.5849995053433545 2316 24-25 0.6219942880083167 2316 26-27 0.6604193808695058 2316 28-29 0.7179851423667643 2316 30-31 0.6360065277640459 2316 32-33 0.6495732165153854 2316 34-35 0.6205170229164949 2316 36-37 0.47748852228208705 2316 38-39 0.5764548556585112 2316 40-41 0.24760552674649006 2316 42-43 0.2031388887497485 2316 44-45 0.434258625082208 2316 46-47 0.46474922785958483 2316 48-49 -4.948690973933758 2316 50-51 -0.8951711110058227 2316 52-53 0.3881660881701805 2316 54-55 0.6830513378615777 2316 56-57 0.5542546594585147 2316 58-59 0.4324453468658689 2316 60-61 0.5553866492873212 2316 62-63 0.50450976662259 2316 64-65 0.5545795139582097 2316 66-67 0.6154325597910031 2316 68-69 0.3100255098823581 2316 70-71 -0.1346348930832164 2316 72-73 -0.011255138266484721 2316 74-75 0.24186695352512544 2316 76-77 0.3358816840021177 2316 78-79 0.4053707694706219 2316 80-81 0.34164928112031845 2316 82-83 0.2910965463034323 2316 84-85 0.3910515962496426 2316 86-87 0.37137597453867244 2316 88-89 0.26734665017115233 2316 90-91 0.35092556689968646 2316 92-93 0.40113024482669957 2316 94-95 0.6287657685233903 2316 96-97 0.6647660073336539 2316 98-99 0.630534432490446 2316 100 -0.18920130479538244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 16.0 11 18.0 12 75.0 13 344.0 14 1181.0 15 3345.0 16 8302.0 17 17093.0 18 28074.0 19 40984.0 20 57560.0 21 78377.0 22 96735.0 23 102881.0 24 107998.0 25 122449.0 26 147732.0 27 181669.0 28 224493.0 29 276473.0 30 339180.0 31 421225.0 32 531356.0 33 688679.0 34 952480.0 35 1333512.0 36 2058199.0 37 2928927.0 38 2191136.0 39 262709.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.65805926464337 19.758303011734604 13.578386550779028 24.005251172843 2 15.515871260618056 20.39263245339084 40.135381516784385 23.956114769206717 3 18.749899172223838 25.84226695832308 29.518343535778847 25.889490333674235 4 12.644611720513376 16.554558097794228 37.946600311121166 32.85422987057123 5 13.416573999470732 37.75908377099365 34.44871766323855 14.375624566297073 6 31.27314303926302 37.01182151557946 17.740627395723497 13.974408049434023 7 28.184959841590064 31.947506409803918 21.92304418046154 17.94448956814448 8 26.463593237510597 35.410474757004465 19.743451193966816 18.382480811518118 9 26.598190481081524 15.768815271548275 20.350391858422256 37.28260238894794 10-11 25.346427492811813 26.153208482485628 27.67971506664634 20.82064895805621 12-13 25.65329178990688 23.631912446759166 28.1997403359122 22.51505542742175 14-15 22.945591619610543 25.158035492139973 25.659311508077014 26.237061380172467 16-17 22.365872521342798 28.102874863280906 26.510476615193916 23.02077600018238 18-19 22.13688527576272 27.30181212654462 28.594197914038677 21.96710468365399 20-21 23.024681691929782 26.439552661730115 28.43576835401549 22.09999729232461 22-23 23.008459131554407 26.28979176429934 28.188053074304975 22.513696029841277 24-25 22.639930701721568 26.44879496764954 28.510588406731873 22.400685923897015 26-27 22.539494478102725 26.65872869594216 28.239091011717495 22.562685814237625 28-29 22.371180725589003 26.68976380171279 28.46739819544386 22.47165727725435 30-31 22.772182363903138 26.54357277914297 28.322249637124518 22.361995219829375 32-33 22.728906439839466 26.789940935971245 28.0208164797548 22.460336144434486 34-35 22.796400234273616 26.68940189890714 28.295175365651037 22.219022501168205 36-37 22.796090871692005 26.478245624680667 28.132009119546918 22.593654384080406 38-39 22.74086677164023 26.59009058505622 28.31398799428793 22.355054649015624 40-41 22.649236112699604 26.275691715920846 28.564187574540227 22.51088459683932 42-43 22.582114331510677 26.34442531764918 28.23484607541717 22.838614275422977 44-45 22.49140408867206 26.70409844704935 28.13207240399539 22.6724250602832 46-47 22.600103335309598 26.55269786435563 27.80828777664181 23.038911023692965 48-49 22.939284310617968 26.651809695207053 27.65131039914396 22.75759559503102 50-51 22.687586871506888 26.65277012940907 27.592884737586452 23.066758261497586 52-53 22.55439807423632 26.852103309885493 27.510306391534627 23.08319222434356 54-55 22.52647950466304 26.7726495744623 27.54311978265722 23.157751138217435 56-57 22.781730913619064 26.7271349063862 27.303426935451707 23.18770724454302 58-59 22.425533950346914 26.74470098238994 27.66692643514891 23.162838632114237 60-61 22.568136959467644 27.04922521483139 27.346519762867256 23.036118062833708 62-63 22.59291080477907 26.812091319104226 27.290021869029417 23.304976007087287 64-65 22.264524121248595 26.68421718861819 27.428330381606436 23.622928308526784 66-67 22.42827181146085 26.63272236288659 27.32719366553138 23.611812160121183 68-69 22.24346511473302 26.72441403323889 27.409348004907024 23.622772847121063 70-71 22.322418506989454 26.443249612702846 27.41948313964751 23.81484874066019 72-73 22.451227079044344 26.66049480427594 27.30174501890289 23.586533097776822 74-75 22.417045020856836 26.821153314694023 27.149708959432022 23.612092705017123 76-77 22.312688753897984 26.744917559016358 27.134319942084854 23.808073745000808 78-79 22.264241823239388 26.691066439673715 27.145343963099407 23.899347773987493 80-81 22.11180523157846 27.047773934466342 27.078005975893365 23.762414858061835 82-83 22.481733434877796 26.615031903332987 27.385239430662196 23.517995231127024 84-85 22.530157333469187 26.703917513042942 26.93113467103303 23.83479048245484 86-87 22.01253440138531 26.935773815784973 27.251472824548568 23.80021895828115 88-89 22.215947753895023 26.580242652593167 27.152774944544067 24.051034648967743 90-91 22.230621690531414 26.580918355728706 27.201372576989286 23.987087376750598 92-93 22.321371421715085 26.660614847918136 27.064279844299993 23.953733886066786 94-95 21.903508057015053 26.40633435961076 27.327594329761663 24.362563253612525 96-97 22.013505834926058 26.413153847330754 27.238865150546804 24.33447516719638 98-99 21.84189211479329 26.589794910806454 27.128484269336727 24.439828705063533 100 21.883782855146983 26.372353849691503 27.015086006869637 24.728777288291877 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2857.0 1 2042.5 2 2737.0 3 3666.5 4 2404.0 5 1817.5 6 2596.5 7 4655.5 8 6935.0 9 7916.0 10 7172.0 11 6456.0 12 6890.0 13 7689.5 14 8813.0 15 10046.0 16 11319.0 17 12517.0 18 13581.0 19 14776.5 20 16257.5 21 18150.0 22 20774.5 23 24293.0 24 28330.0 25 34144.0 26 42624.0 27 53007.0 28 65885.5 29 84698.5 30 109951.5 31 136157.0 32 169903.0 33 209414.5 34 246635.0 35 284698.0 36 329833.5 37 378146.0 38 401583.5 39 403547.0 40 399090.5 41 394236.0 42 401360.5 43 421527.5 44 446866.5 45 481463.5 46 511492.5 47 527726.5 48 545163.0 49 553583.0 50 545413.0 51 533560.0 52 519173.0 53 500499.5 54 481670.5 55 446690.0 56 402296.0 57 351928.0 58 303492.0 59 269623.0 60 226731.5 61 179816.5 62 143521.5 63 112285.5 64 82912.5 65 58271.0 66 41836.5 67 31375.0 68 23734.0 69 17532.0 70 13484.0 71 10588.0 72 8171.0 73 6451.0 74 5154.0 75 3870.5 76 2688.0 77 1630.5 78 978.0 79 670.0 80 438.0 81 251.0 82 157.0 83 112.5 84 78.5 85 48.5 86 34.0 87 20.5 88 10.5 89 7.0 90 6.5 91 3.0 92 1.5 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029401920849756413 2 0.0017798689849801022 3 9.088692689260097E-5 4 0.02436527031779144 5 4.317129027398546E-4 6 3.786955287191707E-5 7 5.3017374020683895E-5 8 0.0038399726612123905 9 0.009520405591999952 10-11 9.959692405314188E-4 12-13 5.3017374020683895E-5 14-15 1.0603474804136779E-4 16-17 3.938433498679375E-4 18-19 1.5147821148766826E-5 20-21 8.709997160540926E-5 22-23 2.3857818309307754E-4 24-25 3.786955287191707E-5 26-27 9.467388217979267E-5 28-29 0.0010489866145521028 30-31 0.002597851327013511 32-33 0.009656735982338852 34-35 0.018294780992423137 36-37 0.021074406173221847 38-39 0.020847188855990344 40-41 0.004673102824394567 42-43 0.005165407011729488 44-45 0.008766801489848802 46-47 0.011633526642252924 48-49 1.2183165332635928 50-51 0.017635850772451778 52-53 0.018404602695751695 54-55 0.009209875258450231 56-57 0.007358054123013486 58-59 0.008168462554472513 60-61 0.004968485336795519 62-63 0.007475449736916429 64-65 0.0187227069398758 66-67 0.028428673340948143 68-69 0.028565003731287043 70-71 0.025796739416349905 72-73 0.022642205662119216 74-75 0.009039462270526604 76-77 0.013905699814567948 78-79 0.007929884371379434 80-81 0.002571342640003169 82-83 0.013458839090679325 84-85 0.023263266329218657 86-87 0.02262705784097045 88-89 0.032003559132057116 90-91 0.02005571520096728 92-93 0.012709021943815369 94-95 0.006142441475824949 96-97 0.003643050986278422 98-99 0.015234921120372237 100 0.0345673278614859 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.3203219E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.97581417652893 #Duplication Level Percentage of deduplicated Percentage of total 1 70.03471537992915 35.00041921733432 2 15.670839375701703 15.663259132606019 3 6.016881903196033 9.02095715748734 4 2.8978989234681474 5.792994324064295 5 1.596426020819355 3.98913450815218 6 0.9760923296236719 2.926860533064471 7 0.6191389361531658 2.16593806958511 8 0.43391056815898255 1.734802713883632 9 0.30514347033598177 1.3724814033622974 >10 1.3284577496386138 11.329811183000112 >50 0.06975420797236262 2.4054399523517622 >100 0.04437202376053619 4.430123726249002 >500 0.004367605915179136 1.4689366234825194 >1k 0.001871537456263668 1.5164071445109804 >5k 5.198714833033233E-5 0.15615880437823515 >10k+ 7.79807224954985E-5 1.0262755064877576 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34143 0.2585960287411729 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26791 0.20291263819830602 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18068 0.1368454162579595 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.786955287191707E-5 2 0.0 0.0 0.0 0.0 3.786955287191707E-5 3 0.0 0.0 0.0 0.0 3.786955287191707E-5 4 0.0 0.0 0.0 0.0 3.786955287191707E-5 5 0.0 7.573910574383414E-6 0.0 0.0 4.544346344630048E-5 6 0.0 7.573910574383414E-6 0.0 0.0 5.3017374020683895E-5 7 0.0 1.5147821148766828E-5 0.0 0.0 6.059128459506731E-5 8 0.0 1.5147821148766828E-5 0.0 0.0 7.573910574383414E-5 9 0.0 1.5147821148766828E-5 0.0 0.0 8.331301631821755E-5 10-11 0.0 2.272173172315024E-5 0.0 1.5147821148766828E-5 8.709997160540926E-5 12-13 0.0 3.0295642297533655E-5 0.0 1.5147821148766828E-5 1.4011734562609316E-4 14-15 0.0 3.0295642297533655E-5 0.0 3.0295642297533655E-5 1.5526516677485998E-4 16-17 0.0 3.0295642297533655E-5 0.0 5.3017374020683895E-5 1.8177385378520192E-4 18-19 0.0 3.0295642297533655E-5 0.0 1.098217033285595E-4 2.234303619443107E-4 20-21 0.0 5.3017374020683895E-5 0.0 2.802346912521863E-4 2.5372600424184436E-4 22-23 0.0 5.3017374020683895E-5 0.0 9.278040453619683E-4 2.9159555711376144E-4 24-25 0.0 5.3017374020683895E-5 0.0 0.002003299346924413 3.4839988642163704E-4 26-27 0.0 5.3017374020683895E-5 0.0 0.003915711766956225 3.6733466285759556E-4 28-29 0.0 5.3017374020683895E-5 0.0 0.00889934492490051 3.7490857343197895E-4 30-31 0.0 6.816519516945072E-5 0.0 0.02584975679037059 3.824824840063624E-4 32-33 0.0 6.816519516945072E-5 0.0 0.06025045861922006 3.938433498679375E-4 34-35 0.0 7.573910574383414E-5 0.0 0.11134784630929775 4.014172604423209E-4 36-37 0.0 8.709997160540926E-5 0.0 0.19144195063340236 4.089911710167043E-4 38-39 0.0 9.846083746698438E-5 0.0 0.30524374396880033 4.2035203687827943E-4 40-41 0.0 9.846083746698438E-5 0.0 0.4304480596739325 4.279259474526629E-4 42-43 0.0 1.0603474804136779E-4 0.0 0.5546261104962358 4.39286813314238E-4 44-45 0.0 1.0603474804136779E-4 0.0 0.7036200793155063 4.847302767605385E-4 46-47 0.0 1.0603474804136779E-4 0.0 0.8795052176291251 5.30173740206839E-4 48-49 0.0 1.0603474804136779E-4 0.0 1.0692543992491528 5.339606954940306E-4 50-51 0.0 1.0603474804136779E-4 0.0 1.277165818426552 5.566824272171809E-4 52-53 0.0 1.1739561390294292E-4 0.0 1.4802640174339303 5.907650248019062E-4 54-55 0.0 1.9692167493396876E-4 0.0 1.6961962079096016 6.210606670994399E-4 56-57 0.0 2.272173172315024E-4 0.0 1.9453930136279647 6.248476223866316E-4 58-59 0.0 2.272173172315024E-4 0.0 2.2241318575417104 6.286345776738234E-4 60-61 0.0 2.272173172315024E-4 0.0 2.5128228199501956 6.286345776738234E-4 62-63 0.0 2.272173172315024E-4 0.0 2.8036041816772106 6.437823988225902E-4 64-65 0.0 2.272173172315024E-4 0.0 3.102769862410068 6.551432646841653E-4 66-67 0.0 2.3479122780588582E-4 0.0 3.431072377122579 6.665041305457404E-4 68-69 0.0 2.4236513838026924E-4 0.0 3.7932605677448814 6.85438906981699E-4 70-71 0.0 2.5372600424184436E-4 0.0 4.181480289011339 7.005867281304658E-4 72-73 0.0 2.575129595290361E-4 0.0 4.5691281800294306 7.422432362895746E-4 74-75 0.0 2.650868701034195E-4 0.0 4.951319068478679 7.687519232999165E-4 76-77 0.0 2.726607806778029E-4 0.0 5.365396120446082 7.801127891614916E-4 78-79 0.0 2.802346912521863E-4 0.0 5.807632971929042 7.801127891614916E-4 80-81 0.0 2.84021646539378E-4 0.0 6.281513621791777 8.14195386746217E-4 82-83 0.0 2.878086018265697E-4 0.0 6.763104512619233 9.012953583516262E-4 84-85 0.0 2.878086018265697E-4 0.0 7.229994443021812 9.050823136388179E-4 86-87 0.0 2.953825124009531E-4 0.0 7.704829405616918 9.315910006491598E-4 88 0.0 2.953825124009531E-4 0.0 8.083884695088372 9.543127323723102E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20970 0.0 47.042732 1 GTATCAA 36830 0.0 39.501923 1 ATCAACG 45620 0.0 31.215797 3 CAACGCA 45945 0.0 31.069508 5 TATCAAC 48435 0.0 29.631369 2 TCAACGC 48820 0.0 29.34292 4 AACGCAG 49400 0.0 29.25738 6 TACCTGG 10040 0.0 28.832546 2 GTACATG 55620 0.0 26.30894 1 GTACTAG 5355 0.0 25.678307 1 ACGCAGA 56100 0.0 25.503962 7 TACATGG 56940 0.0 25.320755 2 CGCAGAG 57900 0.0 24.654379 8 ACATGGG 56350 0.0 24.555676 3 ACCTGGG 12365 0.0 23.261482 3 TAGTACT 7375 0.0 22.07709 4 GCAGAGT 64360 0.0 21.95992 9 CATGGGG 35830 0.0 21.843565 4 GTACCTG 13735 0.0 21.628843 1 TCTAACG 2780 0.0 21.43337 2 >>END_MODULE