FastQCFastQC Report
Mon 6 Jun 2016
ERR1211355_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211355_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8249544
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286370.34713433857677467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221900.26898456448017005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146350.177403744982753No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131550.049.0966531
GTATCAA230200.040.5014461
TACCTGG79350.039.776272
ACCTGGG94550.033.0509763
GTACCTG97550.032.6944961
ATCAACG283750.031.9959813
CAACGCA284250.031.9731525
TCAACGC289400.031.4685544
TATCAAC292800.031.3586392
AACGCAG301850.030.3737346
GTACATG294650.028.8166181
TACATGG305150.027.3456822
ACGCAGA341950.026.5652857
ACATGGG307300.026.5008723
CGCAGAG350450.025.9067618
CATGGGG208750.024.5820274
GCAGAGT387300.022.859719
ATGGGGG140200.022.7588985
GTGGTAT65650.022.5708561
GTACTAG27000.022.1264721
Produced by FastQC (version 0.11.5)