FastQCFastQC Report
Mon 6 Jun 2016
ERR1211355_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211355_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8249544
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248410.30111967400864825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213210.2584506489085942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140530.17034880958268725No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129650.050.1318241
TACCTGG82900.042.1042182
GTATCAA227150.040.7202221
ACCTGGG98200.034.4979673
GTACCTG101850.034.3266531
CAACGCA281800.032.028735
ATCAACG282500.031.8730963
TCAACGC290100.031.021924
TATCAAC298300.030.5738562
AACGCAG303850.030.04416
GTACATG291650.027.8529571
TACATGG303050.026.2399252
ACGCAGA350900.025.8953537
CGCAGAG360450.025.131188
ACATGGG305550.025.0921273
CCTGGGG95550.023.6201234
CATGGGG208900.023.3145314
TGGGGGG128450.022.9351256
GCAGAGT394800.022.6943979
TAACGCA10350.022.6671734
Produced by FastQC (version 0.11.5)