##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211355_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8249544 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10758049172173 33.0 31.0 34.0 30.0 34.0 2 32.22587539383995 33.0 31.0 34.0 30.0 34.0 3 32.29666415501269 34.0 31.0 34.0 30.0 34.0 4 35.93902850872727 37.0 35.0 37.0 35.0 37.0 5 35.84754963425857 37.0 35.0 37.0 35.0 37.0 6 35.86501023571727 37.0 35.0 37.0 35.0 37.0 7 35.77977461542117 37.0 35.0 37.0 33.0 37.0 8 35.75961519812489 37.0 35.0 37.0 33.0 37.0 9 37.38009858484299 39.0 37.0 39.0 34.0 39.0 10-11 37.324998751446145 39.0 37.0 39.0 34.0 39.0 12-13 37.25584268657758 39.0 37.0 39.0 33.5 39.0 14-15 38.49663848086634 40.0 38.0 41.0 34.0 41.0 16-17 38.47890992520314 40.0 38.0 41.0 34.0 41.0 18-19 38.479923496377495 40.0 38.0 41.0 34.0 41.0 20-21 38.44150682752889 40.0 38.0 41.0 34.0 41.0 22-23 38.33127267398052 40.0 38.0 41.0 34.0 41.0 24-25 38.24548156843578 40.0 38.0 41.0 34.0 41.0 26-27 38.14869670372084 40.0 38.0 41.0 33.0 41.0 28-29 38.03740503717539 40.0 37.0 41.0 33.0 41.0 30-31 38.04061569948594 40.0 37.0 41.0 33.0 41.0 32-33 37.969488374145286 40.0 37.0 41.0 33.0 41.0 34-35 37.83371226336874 40.0 37.0 41.0 33.0 41.0 36-37 37.68672656331066 40.0 37.0 41.0 33.0 41.0 38-39 37.571328427365195 40.0 37.0 41.0 32.0 41.0 40-41 37.45919422940226 40.0 36.0 41.0 32.0 41.0 42-43 37.353164611280334 40.0 36.0 41.0 31.5 41.0 44-45 37.20433850646775 40.0 36.0 41.0 31.0 41.0 46-47 36.967548933613784 39.0 36.0 41.0 31.0 41.0 48-49 35.398725068925025 39.0 35.0 41.0 24.0 41.0 50-51 36.157256873834484 39.0 35.0 40.0 29.0 41.0 52-53 36.2601511671433 39.0 35.0 40.0 29.5 41.0 54-55 36.095777354481655 38.5 35.0 40.0 29.0 41.0 56-57 35.887495478537964 38.0 35.0 40.0 28.5 41.0 58-59 35.654606545525425 38.0 34.0 40.0 28.0 41.0 60-61 35.39978003632686 37.5 34.0 40.0 28.0 41.0 62-63 35.0768969775784 37.0 34.0 40.0 27.5 41.0 64-65 34.74146758899643 36.5 34.0 39.5 26.5 41.0 66-67 34.40219707901431 36.0 33.0 39.0 26.0 40.5 68-69 34.0357068827077 35.5 33.0 39.0 26.0 40.0 70-71 33.665600971399144 35.0 32.5 38.0 26.0 40.0 72-73 33.2824617942519 35.0 32.0 37.5 26.0 39.5 74-75 32.91248595073861 35.0 32.0 37.0 25.0 39.0 76-77 31.97628371943952 34.0 30.5 36.0 24.5 38.0 78-79 32.41031128508436 34.5 31.5 36.0 25.0 38.0 80-81 32.28478149822584 35.0 32.0 35.5 25.0 37.0 82-83 32.01061149561721 34.0 31.0 35.0 24.5 37.0 84-85 31.72672307705735 34.0 31.0 35.0 24.0 36.5 86-87 31.4693234559389 34.0 31.0 35.0 24.0 36.0 88-89 31.242963550470186 34.0 31.0 35.0 23.0 36.0 90-91 31.023016544914483 34.0 30.5 35.0 22.5 35.0 92-93 30.83379202535316 34.0 30.0 35.0 20.5 35.0 94-95 30.635107952633504 34.0 30.0 35.0 20.0 35.0 96-97 30.446866032837697 34.0 29.5 35.0 20.0 35.0 98-99 30.22665089124926 34.0 29.0 35.0 19.0 35.0 100 27.813498540040516 31.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3216030748682357 1101 2 -0.27346956831741664 1101 3 -0.32322507014498925 1101 4 -0.18771552484051313 1101 5 -0.2025260507269948 1101 6 -0.23942185415207717 1101 7 -0.2217244762614925 1101 8 -0.21853513308991523 1101 9 -0.3351333086428028 1101 10-11 -0.33764068761140464 1101 12-13 -0.4431031405374455 1101 14-15 -0.5349086157543823 1101 16-17 -0.4914263529607652 1101 18-19 -0.5078065047117306 1101 20-21 -0.5923581414776962 1101 22-23 -0.6496538011378732 1101 24-25 -0.5705699174899195 1101 26-27 -0.6532597487638512 1101 28-29 -0.6183015583226776 1101 30-31 -0.5525378696989804 1101 32-33 -0.47397723111001255 1101 34-35 -0.48065597228981005 1101 36-37 -0.476096686667816 1101 38-39 -0.5170182144071092 1101 40-41 -0.5083335664427935 1101 42-43 -0.6070438730951366 1101 44-45 -0.6382078498620771 1101 46-47 -0.5398019992484109 1101 48-49 0.690391426885256 1101 50-51 -0.3125616681218659 1101 52-53 -0.559744090657567 1101 54-55 -0.6032001243338456 1101 56-57 -0.6855148870522996 1101 58-59 -0.7129092253497973 1101 60-61 -0.7112386350446371 1101 62-63 -0.816148562692895 1101 64-65 -0.9003422974238404 1101 66-67 -0.8475555200869138 1101 68-69 -0.8562636512643635 1101 70-71 -0.7763000228430954 1101 72-73 -0.8828830452009129 1101 74-75 -0.9952958766000393 1101 76-77 -0.9021838563214679 1101 78-79 -0.816761028066697 1101 80-81 -0.6931199965509585 1101 82-83 -0.6615506120927819 1101 84-85 -0.7045936026166366 1101 86-87 -0.7620405652693698 1101 88-89 -0.7497650633153015 1101 90-91 -0.8833322780857848 1101 92-93 -0.7744764405590132 1101 94-95 -0.8473703091867435 1101 96-97 -0.8649130911126619 1101 98-99 -0.9642921878335962 1101 100 -0.9814879473534397 1102 1 -0.2641046311712181 1102 2 -0.26955306466971507 1102 3 -0.2801234285497358 1102 4 -0.16398638341440375 1102 5 -0.17466412349831018 1102 6 -0.29490235593714687 1102 7 -0.2105013096349424 1102 8 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1105 68-69 -0.6301171068950282 1105 70-71 -0.7100357425778796 1105 72-73 -0.6820032871146537 1105 74-75 -0.7258151777288901 1105 76-77 -0.7239914046184346 1105 78-79 -0.6264408319057466 1105 80-81 -0.5739429015293034 1105 82-83 -0.6401098754617323 1105 84-85 -0.5851952213848115 1105 86-87 -0.5387499008471401 1105 88-89 -0.5551159383582451 1105 90-91 -0.6449330137862717 1105 92-93 -0.5694677144322711 1105 94-95 -0.6049038375787497 1105 96-97 -0.6829373099051779 1105 98-99 -0.693581813401547 1105 100 -0.7520173323757966 1106 1 -0.25387183497257126 1106 2 -0.2967896966582799 1106 3 -0.3033891252647152 1106 4 -0.22196063852683778 1106 5 -0.21996068866862117 1106 6 -0.3154454147721779 1106 7 -0.3082563871295676 1106 8 -0.368409123759335 1106 9 -0.43894424628314965 1106 10-11 -0.43751429964584787 1106 12-13 -0.45301793166306226 1106 14-15 -0.5925896439677203 1106 16-17 -0.5645117646976061 1106 18-19 -0.5877718896677706 1106 20-21 -0.6395425688377614 1106 22-23 -0.6284482515669652 1106 24-25 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0.32647179253716274 2311 2 0.3770813910373647 2311 3 0.4167921829531096 2311 4 0.294066923245218 2311 5 0.3322329915463982 2311 6 0.3705461803829593 2311 7 0.3913736173538851 2311 8 0.16115013920672538 2311 9 0.43409347649355823 2311 10-11 0.3749512336267742 2311 12-13 0.5122001678121393 2311 14-15 0.4462358545982923 2311 16-17 0.4443838948935408 2311 18-19 0.6322667711161145 2311 20-21 0.6848096103472159 2311 22-23 0.7099179068958108 2311 24-25 0.6103894984945626 2311 26-27 0.7210844965647496 2311 28-29 0.7253240693842784 2311 30-31 0.6279357821667944 2311 32-33 0.6499615000622612 2311 34-35 0.7612277002732597 2311 36-37 0.8141757287410769 2311 38-39 0.8130237191711629 2311 40-41 0.7646948249107623 2311 42-43 0.8454222146871544 2311 44-45 0.8605581488522276 2311 46-47 0.9113330071379551 2311 48-49 -6.120501767390763 2311 50-51 -1.04256893704909 2311 52-53 0.36019325998812945 2311 54-55 0.8344281582039557 2311 56-57 0.9695882410041321 2311 58-59 0.9806064807380679 2311 60-61 0.8964839381313539 2311 62-63 0.7550899386501513 2311 64-65 0.8524611492328944 2311 66-67 0.9344657676414698 2311 68-69 0.9344991637494999 2311 70-71 0.9682199083238672 2311 72-73 0.3995509144199403 2311 74-75 0.7481001763773278 2311 76-77 0.8856962770326824 2311 78-79 0.8115482470929223 2311 80-81 0.820726837166518 2311 82-83 0.8251926091750974 2311 84-85 0.9500676999743938 2311 86-87 0.8888301744687972 2311 88-89 0.9668076802193575 2311 90-91 0.9726918968537106 2311 92-93 0.9952040844543433 2311 94-95 1.0803786992888043 2311 96-97 1.0405815158777862 2311 98-99 1.1051981140928895 2311 100 1.2775416466209855 2312 1 0.08530804561763006 2312 2 0.27034256004889556 2312 3 0.21873913607231543 2312 4 0.06590437039305641 2312 5 0.22419024208006988 2312 6 0.16131523600857633 2312 7 0.30266955649456406 2312 8 0.36017433169162416 2312 9 0.3120161856150361 2312 10-11 0.2900010413808971 2312 12-13 0.3346384734727792 2312 14-15 0.397039815316667 2312 16-17 0.5315632404127442 2312 18-19 0.3320420850352903 2312 20-21 0.3586222654008324 2312 22-23 0.6407904595213196 2312 24-25 0.7204104693671454 2312 26-27 0.6644630118001871 2312 28-29 0.7603964500599645 2312 30-31 0.7867473219676597 2312 32-33 0.7756227960014002 2312 34-35 0.3499375563258056 2312 36-37 0.7068680029500456 2312 38-39 0.8076401134379907 2312 40-41 0.5368884188784122 2312 42-43 0.5937591230108055 2312 44-45 0.7060145756957681 2312 46-47 0.8294000500543248 2312 48-49 -4.657169629155057 2312 50-51 -0.7700973669792788 2312 52-53 0.47048538151306474 2312 54-55 0.7822783733215246 2312 56-57 0.8787377049616367 2312 58-59 0.9206664751540856 2312 60-61 0.9163503756689622 2312 62-63 0.6377252989102686 2312 64-65 0.904661931815788 2312 66-67 0.8655081194287604 2312 68-69 0.820702173747101 2312 70-71 0.8122715803272627 2312 72-73 0.9182074443921664 2312 74-75 0.5660275680149383 2312 76-77 0.7696544867352308 2312 78-79 0.4765796390107653 2312 80-81 0.6901137417812393 2312 82-83 0.7102321375442386 2312 84-85 0.7787331339390668 2312 86-87 0.8491142986270752 2312 88-89 0.826760332202813 2312 90-91 0.6331673654143977 2312 92-93 0.8381192546733374 2312 94-95 0.9646287935572779 2312 96-97 1.0839895737205119 2312 98-99 1.1836493834720194 2312 100 0.6805043554539942 2313 1 0.07463126671102316 2313 2 0.2369469479113988 2313 3 0.2997082977074612 2313 4 0.07887163089898053 2313 5 0.1663565702995342 2313 6 0.28423019537482475 2313 7 0.14928317633299315 2313 8 0.2566839317414349 2313 9 0.43684012025958197 2313 10-11 0.29048969335674 2313 12-13 0.05067333227903248 2313 14-15 0.19470299808619274 2313 16-17 0.139454991791645 2313 18-19 0.45284366383624786 2313 20-21 0.3864631254182669 2313 22-23 0.5246749182167036 2313 24-25 0.3413056044648286 2313 26-27 0.4764926881964513 2313 28-29 0.4113995769304566 2313 30-31 0.5904777294400176 2313 32-33 0.5410921303751905 2313 34-35 0.41852690688880756 2313 36-37 -0.22123007568419695 2313 38-39 0.24491163647047642 2313 40-41 0.4713303739281969 2313 42-43 0.5414759350992995 2313 44-45 0.5066193680378248 2313 46-47 0.43456686836943703 2313 48-49 -5.190045205117876 2313 50-51 -1.2311713568600098 2313 52-53 0.1322278987668497 2313 54-55 0.2521544383756975 2313 56-57 0.26779547222445643 2313 58-59 0.42382300372023707 2313 60-61 0.7293035635001246 2313 62-63 0.8267684599669494 2313 64-65 0.872950207482539 2313 66-67 0.8968719813021266 2313 68-69 0.9055575151871054 2313 70-71 0.8678965891148351 2313 72-73 0.609786197641057 2313 74-75 0.7847350110413203 2313 76-77 0.7918351437245157 2313 78-79 0.7509216002369641 2313 80-81 -0.2033739365312215 2313 82-83 0.3032799788637206 2313 84-85 0.6326311204795125 2313 86-87 0.7223409658561515 2313 88-89 0.7366225593360305 2313 90-91 0.7719699477564959 2313 92-93 0.8580347571117599 2313 94-95 0.806542158857301 2313 96-97 0.7583527757724831 2313 98-99 1.0088896420677713 2313 100 1.086283718008275 2314 1 0.02242992681670586 2314 2 0.1494674138396661 2314 3 0.14321996982056362 2314 4 0.007542807811553587 2314 5 0.15715256949390266 2314 6 0.2667624683073768 2314 7 0.11401321931237618 2314 8 0.20479368386619257 2314 9 0.3999910445428867 2314 10-11 0.31010493390525795 2314 12-13 0.2218510809563199 2314 14-15 0.22823014614468917 2314 16-17 0.43503433889849674 2314 18-19 0.4961728736365103 2314 20-21 0.5327412807014227 2314 22-23 0.2889250446229994 2314 24-25 0.41971674115034574 2314 26-27 0.47122960951141835 2314 28-29 0.5926508728891449 2314 30-31 0.49147611619595466 2314 32-33 0.4758695487734528 2314 34-35 0.354149098358981 2314 36-37 0.43297755070909716 2314 38-39 0.45718301201620903 2314 40-41 0.22901876885192962 2314 42-43 0.2939212868283718 2314 44-45 0.5305593636331096 2314 46-47 0.6209750889775947 2314 48-49 -6.447113397875764 2314 50-51 -1.549962454388691 2314 52-53 -0.817039204853117 2314 54-55 -0.4300848518960052 2314 56-57 -0.10280405901320222 2314 58-59 0.33401978629917295 2314 60-61 0.5681323500467812 2314 62-63 0.5275849232315792 2314 64-65 0.6790480012375113 2314 66-67 0.7633350353624024 2314 68-69 0.8155354557096288 2314 70-71 0.55059787240193 2314 72-73 0.7532796055621631 2314 74-75 0.758399891793303 2314 76-77 0.6648458084271418 2314 78-79 0.5044055769232116 2314 80-81 0.5220361066562518 2314 82-83 0.502123983712373 2314 84-85 0.5309188013841997 2314 86-87 0.6072027315012356 2314 88-89 0.601968890283441 2314 90-91 0.7354487480399996 2314 92-93 0.7554079897461641 2314 94-95 0.8607053355706995 2314 96-97 0.8693092285876993 2314 98-99 0.9537542021528154 2314 100 -0.3412368581616434 2315 1 0.17644653516672548 2315 2 0.21705389394104913 2315 3 0.2925932747819502 2315 4 0.14951855029433858 2315 5 0.2212343739892617 2315 6 0.282026101357161 2315 7 0.3407697168787607 2315 8 0.2731520889189625 2315 9 0.42887771275912456 2315 10-11 0.2330237089382976 2315 12-13 0.39381864743517525 2315 14-15 0.27112724123460197 2315 16-17 0.38037170990084945 2315 18-19 0.4261145126160599 2315 20-21 0.3676584424419005 2315 22-23 0.4393779590999429 2315 24-25 0.51586537395346 2315 26-27 0.6027861841723521 2315 28-29 0.661947623412324 2315 30-31 0.6276032733461037 2315 32-33 0.6833019065433348 2315 34-35 0.71712634275422 2315 36-37 0.6301209906243201 2315 38-39 0.7211759982673627 2315 40-41 0.7188579821037493 2315 42-43 0.7519375906221413 2315 44-45 0.7532732048549704 2315 46-47 0.67060166575979 2315 48-49 -4.988618091632027 2315 50-51 -0.9098307404086583 2315 52-53 0.31372140778068314 2315 54-55 0.3170276206697409 2315 56-57 0.6509232568835941 2315 58-59 0.7625097957908693 2315 60-61 0.5988068469832655 2315 62-63 0.353415280764942 2315 64-65 0.54281328192139 2315 66-67 -0.14290775601352834 2315 68-69 0.6029088307933179 2315 70-71 0.5361250241629776 2315 72-73 0.6272247727246807 2315 74-75 0.7808447436453605 2315 76-77 0.784225020612336 2315 78-79 0.6861557634829012 2315 80-81 0.5554537786423737 2315 82-83 0.5330629984229418 2315 84-85 0.6456638952421336 2315 86-87 0.6624045505871514 2315 88-89 0.6369938580587657 2315 90-91 0.7113187422615042 2315 92-93 0.7213091340189095 2315 94-95 0.39784343715624004 2315 96-97 0.45015786789131695 2315 98-99 0.42094136495911627 2315 100 0.44532198487963015 2316 1 0.2560903584269312 2316 2 0.27758382666210224 2316 3 0.2158525792605701 2316 4 -0.028916032577022577 2316 5 0.16290038272655494 2316 6 0.11723459413867232 2316 7 0.22450202587332768 2316 8 0.3519801257025321 2316 9 0.45438263966712356 2316 10-11 0.4263790420252107 2316 12-13 0.35976348105630507 2316 14-15 0.49637822742462134 2316 16-17 0.5021091326461757 2316 18-19 0.15084162028256998 2316 20-21 0.4910344047411641 2316 22-23 0.6248173826313064 2316 24-25 0.6483296434648551 2316 26-27 0.6584503377358857 2316 28-29 0.6922505835943085 2316 30-31 0.5971726742084016 2316 32-33 0.5666358293708811 2316 34-35 0.5492891017273749 2316 36-37 0.4037516114722166 2316 38-39 0.5092843279920061 2316 40-41 0.11507007737108665 2316 42-43 0.12257266081879692 2316 44-45 0.43341581604482116 2316 46-47 0.4405522622711544 2316 48-49 -5.030190855250311 2316 50-51 -0.9797661009889893 2316 52-53 0.31687024727892776 2316 54-55 0.6204766216966178 2316 56-57 0.511068620446899 2316 58-59 0.37793427702234084 2316 60-61 0.5368001770859365 2316 62-63 0.4667154903655799 2316 64-65 0.494398947191776 2316 66-67 0.5796799996703186 2316 68-69 0.35725681181729385 2316 70-71 -0.15918738706210434 2316 72-73 0.06681463599478121 2316 74-75 0.3003563064595909 2316 76-77 0.33407505604008847 2316 78-79 0.39679549869260455 2316 80-81 0.22238220568753775 2316 82-83 0.17789129047458374 2316 84-85 0.3031421063246391 2316 86-87 0.37287325205788235 2316 88-89 0.19743410625841662 2316 90-91 0.2508254041337068 2316 92-93 0.31679441639310824 2316 94-95 0.49440120677899557 2316 96-97 0.6046188614559895 2316 98-99 0.61478969705054 2316 100 -0.06291301960351348 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 5.0 11 15.0 12 51.0 13 204.0 14 729.0 15 2182.0 16 5263.0 17 10820.0 18 18620.0 19 26551.0 20 36578.0 21 48944.0 22 60950.0 23 66025.0 24 68712.0 25 75439.0 26 88998.0 27 109344.0 28 135177.0 29 165411.0 30 203305.0 31 251860.0 32 318098.0 33 412639.0 34 571178.0 35 800106.0 36 1239840.0 37 1854252.0 38 1489166.0 39 189060.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48622573915511 19.065148473672817 13.27884943589159 24.169776351280483 2 15.96964475641933 20.199767691688905 39.95821747616973 23.872370075722035 3 19.028320990213388 25.340555558586033 29.842263836688975 25.788859614511605 4 12.821732442861657 16.3036970449878 37.267966226353465 33.60660428579708 5 13.242241774109518 37.265837380244285 34.481584464128865 15.010336381517334 6 31.529665525674034 36.26249796655378 17.96355482522545 14.244281682546738 7 28.174166738690857 31.945466554935226 21.777824541310956 18.102542165062964 8 26.369224223153353 35.561141272230145 19.879006669119427 18.190627835497075 9 26.82387262399269 15.867143864399678 20.20956726334972 37.09941624825791 10-11 25.381523572913732 26.002000737624265 27.94323747955547 20.67323820990653 12-13 25.518235458690263 23.563257891477964 28.297653733569373 22.6208529162624 14-15 22.879856874296436 25.23251774964433 25.863977851348285 26.023647524710945 16-17 22.4018158145182 27.951538034753774 26.756553501712403 22.89009264901562 18-19 22.118799664490528 27.10492404822158 28.79654492397064 21.979731363317256 20-21 22.933401312885405 26.483411589216587 28.678231069746378 21.90495602815163 22-23 22.826561884907953 26.222889735199985 28.502226339629754 22.448322040262305 24-25 22.465006085489723 26.23311853149657 28.80278255464258 22.49909282837113 26-27 22.409612514058363 26.46916712184367 28.596086229313688 22.525134134784274 28-29 22.292295419256682 26.69150584116384 28.72293356093586 22.29326517864362 30-31 22.841909738246272 26.42337506889963 28.68919846595822 22.04551672689588 32-33 22.684815095799877 26.516482845053762 28.493465319263823 22.305236739882535 34-35 22.79248941281793 26.613931864127867 28.542257221952767 22.05132150110144 36-37 22.644898894495757 26.55954543139884 28.409422126089073 22.386133548016325 38-39 22.552280174535298 26.683347388520723 28.495123786584482 22.269248650359497 40-41 22.651989625616654 26.35067912934218 28.695213394325364 22.3021178507158 42-43 22.42500338672386 26.49679405294321 28.375339316393955 22.702863243938975 44-45 22.21624559966618 26.76775281174027 28.48442550352136 22.531576085072192 46-47 22.336268453306765 26.52421384568486 28.149765131603953 22.989752569404427 48-49 22.65044513269916 26.734739986125 27.973727334545913 22.641087546629926 50-51 22.462267676640618 26.77853327461251 27.977456537546523 22.78174251120035 52-53 22.36240379932427 26.9885352715503 27.785080564447618 22.863980364677808 54-55 22.261434171750157 26.82237974891017 27.997628977248823 22.918557102090848 56-57 22.530830579182407 26.883010336210983 27.643947005135526 22.94221207947108 58-59 22.1756122309504 26.868333711669933 27.985274270797877 22.97077978658179 60-61 22.533418675666404 27.01336989476466 27.78175395465946 22.671457474909477 62-63 22.44896907716404 26.95412854046348 27.75369381688754 22.843208565484936 64-65 22.22242631424299 26.70508729268317 27.745750485865305 23.326735907208533 66-67 22.210725287815418 26.768664623288164 27.60096668827419 23.419643400622228 68-69 22.031825266654998 26.825901118407725 27.78550807969297 23.356765535244307 70-71 22.207385787865284 26.533605170491647 27.868969138471865 23.390039903171196 72-73 22.225214997892124 26.85723756823013 27.498069447510982 23.419477986366765 74-75 22.260920998548446 26.973742153890285 27.412517539771315 23.35281930778996 76-77 22.180828077281166 26.819593037321347 27.481543729632662 23.518035155764817 78-79 22.112320027174697 26.77210924990206 27.51757684511034 23.5979938778129 80-81 22.074832010070182 27.088127677195555 27.264372848909435 23.572667463824825 82-83 22.426680969349455 26.86250899575381 27.42823522223149 23.282574812665246 84-85 22.356812690669774 27.016166335518665 27.14450109007852 23.48251988373304 86-87 22.007670348867382 27.115335556847782 27.394883410988207 23.48211068329663 88-89 22.03396530544699 26.805389423230537 27.404842075280445 23.75580319604203 90-91 22.31290789076052 26.69543580639422 27.38853221312119 23.60312408972407 92-93 22.26766187074127 26.801054334551484 27.29108118876748 23.640202605939763 94-95 22.001939148370063 26.659095631436703 27.387682504338112 23.951282715855122 96-97 21.999859138214383 26.605457654728852 27.424486506059175 23.970196700997583 98-99 21.916051611082324 26.62075097601859 27.315548186500354 24.14764922639873 100 21.850339515731417 26.638288604984318 27.36594530739761 24.14542657188666 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2665.0 1 2011.5 2 2976.0 3 4152.5 4 2796.5 5 2028.5 6 2794.5 7 5113.0 8 7689.0 9 8724.0 10 7686.0 11 6339.0 12 6400.0 13 6943.0 14 7639.0 15 8302.5 16 9061.0 17 9937.0 18 10700.0 19 11638.5 20 12919.5 21 14427.0 22 16431.0 23 19264.5 24 22581.0 25 27041.0 26 33492.0 27 41101.0 28 49807.0 29 61517.0 30 77014.0 31 92222.0 32 110065.0 33 130705.0 34 150797.0 35 171562.0 36 196668.0 37 222079.5 38 234814.0 39 240457.0 40 242741.5 41 244737.5 42 250223.0 43 260743.5 44 275568.0 45 294504.5 46 309125.0 47 316334.0 48 327289.0 49 335149.5 50 337277.5 51 335385.5 52 327225.0 53 316129.0 54 302937.0 55 281493.5 56 253960.0 57 223300.5 58 194345.5 59 169025.5 60 140376.0 61 112486.5 62 89279.0 63 68951.0 64 50819.0 65 36258.0 66 26281.5 67 19754.0 68 15028.0 69 11194.5 70 8611.0 71 6565.0 72 4938.5 73 3849.0 74 2921.5 75 2033.0 76 1356.5 77 813.0 78 479.0 79 295.5 80 198.0 81 121.0 82 73.5 83 45.5 84 31.5 85 22.0 86 15.0 87 9.0 88 5.0 89 3.5 90 2.0 91 1.0 92 0.5 93 0.5 94 1.0 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029807708159384324 2 0.0019637449051729404 3 1.0909693917627447E-4 4 0.023395232512245526 5 5.091190494892809E-4 6 2.4243764261394325E-5 7 1.2121882130697162E-5 8 0.0039880992209993665 9 0.01008540593274004 10-11 0.0010485428043053046 12-13 1.4546258556836597E-4 14-15 1.5152352663371456E-4 16-17 2.7880328900603475E-4 18-19 6.060941065348581E-6 20-21 5.4548469588137235E-5 22-23 2.000110551565032E-4 24-25 3.0304705326742907E-5 26-27 7.273129278418298E-5 28-29 9.091411598022873E-4 30-31 0.002727423479406862 32-33 0.009527799354727971 34-35 0.01794644649449715 36-37 0.020898124793321912 38-39 0.02060113868111983 40-41 0.0045941933275342245 42-43 0.005109373318088854 44-45 0.008945949012454506 46-47 0.011982480486194146 48-49 1.2252192363602157 50-51 0.01789795896597436 52-53 0.0188010391847113 54-55 0.009491433708335878 56-57 0.007654968565535259 58-59 0.008685328546644517 60-61 0.005018459202108625 62-63 0.007564054449555031 64-65 0.019764728814101726 66-67 0.029310710992025743 68-69 0.02930465005096039 70-71 0.026722689157121893 72-73 0.023092185458978096 74-75 0.009582347824316108 76-77 0.01449171008724846 78-79 0.00841864713976918 80-81 0.0029092517113673195 82-83 0.014643233613882175 84-85 0.023916473443865504 86-87 0.023516451333552495 88-89 0.032577558226248624 90-91 0.020625382445381223 92-93 0.013558325163184778 94-95 0.006812497757451805 96-97 0.003915367928215184 98-99 0.015006890077803089 100 0.03391702620169066 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8249544.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.514489744726625 #Duplication Level Percentage of deduplicated Percentage of total 1 74.56796226267797 42.88738300841955 2 15.230891557619145 17.519939125874593 3 4.8446017950549605 8.359044007769183 4 1.999900678972883 4.600930683650308 5 0.9920879578705052 2.852971633940497 6 0.57060555394185 1.9690852368283556 7 0.3684204400158132 1.4832659533326014 8 0.2703783555632945 1.2440538526592917 9 0.18285236759121634 0.9464994558561596 >10 0.8785728784297471 8.96958936808037 >50 0.05452052727465728 2.1686473324144835 >100 0.03550318359161921 3.944843315795108 >500 0.0026739976691656743 1.0324928036845016 >1k 9.109073013737778E-4 0.8995257292992964 >5k 2.9384106479861212E-5 0.10408783235399832 >10k+ 8.815231943958364E-5 1.0176406600415668 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24841 0.30111967400864825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21321 0.2584506489085942 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14053 0.17034880958268725 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2121882130697164E-5 2 0.0 0.0 0.0 0.0 1.2121882130697164E-5 3 0.0 0.0 0.0 0.0 1.2121882130697164E-5 4 0.0 1.2121882130697164E-5 0.0 0.0 2.4243764261394328E-5 5 0.0 1.2121882130697164E-5 0.0 0.0 2.4243764261394328E-5 6 6.0609410653485814E-5 1.2121882130697164E-5 0.0 0.0 6.0609410653485814E-5 7 6.0609410653485814E-5 1.2121882130697164E-5 0.0 0.0 6.0609410653485814E-5 8 6.0609410653485814E-5 1.2121882130697164E-5 0.0 0.0 6.0609410653485814E-5 9 6.0609410653485814E-5 1.2121882130697164E-5 0.0 1.2121882130697164E-5 7.273129278418298E-5 10-11 6.66703517188344E-5 1.2121882130697164E-5 0.0 1.2121882130697164E-5 7.273129278418298E-5 12-13 7.273129278418298E-5 1.2121882130697164E-5 0.0 3.030470532674291E-5 7.273129278418298E-5 14-15 9.697505704557731E-5 3.030470532674291E-5 0.0 6.0609410653485814E-5 1.0909693917627447E-4 16-17 1.6970634982976028E-4 4.8487528522788657E-5 0.0 7.273129278418298E-5 1.3334070343766879E-4 18-19 1.6970634982976028E-4 4.8487528522788657E-5 0.0 8.485317491488016E-5 1.3334070343766879E-4 20-21 1.6970634982976028E-4 4.8487528522788657E-5 0.0 1.5152352663371453E-4 1.3334070343766879E-4 22-23 1.8182823196045744E-4 4.8487528522788657E-5 0.0 4.424486977704465E-4 1.6364540876441172E-4 24-25 1.9395011409115463E-4 4.8487528522788657E-5 0.0 0.0012303710362657622 2.0607199622185178E-4 26-27 1.9395011409115463E-4 4.8487528522788657E-5 0.0 0.002509229601054313 2.303157604832461E-4 28-29 1.9395011409115463E-4 4.8487528522788657E-5 0.0 0.006642791407622046 2.303157604832461E-4 30-31 1.9395011409115463E-4 4.8487528522788657E-5 0.0 0.022449725706051145 2.4243764261394326E-4 32-33 1.9395011409115463E-4 4.8487528522788657E-5 0.0 0.05781531682236012 2.7880328900603475E-4 34-35 1.9395011409115463E-4 4.8487528522788657E-5 0.0 0.1098606177505084 2.7880328900603475E-4 36-37 1.9395011409115463E-4 6.66703517188344E-5 0.0 0.19170756589697563 3.1516893539812625E-4 38-39 1.9395011409115463E-4 8.485317491488014E-5 0.0 0.31000501361044924 3.1516893539812625E-4 40-41 1.9395011409115463E-4 1.1515788024162306E-4 0.0 0.4398182493480852 3.1516893539812625E-4 42-43 1.9395011409115463E-4 1.4546258556836597E-4 0.0 0.5647584884691808 3.2122987646347485E-4 44-45 1.9395011409115463E-4 1.4546258556836597E-4 0.0 0.7142879654923957 3.272908175288234E-4 46-47 1.9395011409115463E-4 1.4546258556836597E-4 0.0 0.8918250511785863 3.3941269965952057E-4 48-49 1.9395011409115463E-4 1.5152352663371453E-4 0.0 1.0787990221035249 3.7577834605161207E-4 50-51 2.0001105515650322E-4 1.5758446769906313E-4 0.0 1.2792040384292758 3.7577834605161207E-4 52-53 2.0607199622185178E-4 1.5758446769906313E-4 0.0 1.479724212635268 4.06083051378355E-4 54-55 2.0607199622185178E-4 1.5758446769906313E-4 0.0 1.7033123285359772 4.424486977704465E-4 56-57 2.0607199622185178E-4 1.5758446769906313E-4 0.0 1.9540655822915787 4.5457057990114366E-4 58-59 2.0607199622185178E-4 1.5758446769906313E-4 0.0 2.238578277781172 4.727534030971893E-4 60-61 2.1819387835254894E-4 1.5758446769906313E-4 0.0 2.525321399582813 4.848752852278865E-4 62-63 2.5455952474464044E-4 1.5758446769906313E-4 0.0 2.8121978620878925 5.212409316199781E-4 64-65 2.5455952474464044E-4 1.5758446769906313E-4 0.0 3.104595841903504 5.212409316199781E-4 66-67 2.7274234794068616E-4 1.5758446769906313E-4 0.0 3.436517218406254 5.212409316199781E-4 68-69 2.8486423007138335E-4 1.5758446769906313E-4 0.0 3.813338046321106 5.212409316199781E-4 70-71 2.9092517113673194E-4 1.6970634982976028E-4 0.0 4.202541376832465 5.394237548160237E-4 72-73 3.0910799433277766E-4 1.8182823196045744E-4 0.0 4.572016344176115 6.303378707962525E-4 74-75 3.1516893539812625E-4 1.8182823196045744E-4 0.0 4.944703610284399 6.303378707962525E-4 76-77 3.33351758594172E-4 1.8182823196045744E-4 0.0 5.346392479390376 6.363988118616011E-4 78-79 3.3941269965952057E-4 1.8182823196045744E-4 0.0 5.790168523254134 6.424597529269497E-4 80-81 3.3941269965952057E-4 1.8182823196045744E-4 0.0 6.236502284247469 6.970082225150869E-4 82-83 3.3941269965952057E-4 1.8182823196045744E-4 0.0 6.683436078406273 7.515566921032241E-4 84-85 3.5153458179021775E-4 1.8182823196045744E-4 0.0 7.13223058147214 7.576176331685727E-4 86-87 3.5153458179021775E-4 1.8182823196045744E-4 0.0 7.5962259247299 7.879223384953156E-4 88 3.5153458179021775E-4 1.8182823196045744E-4 0.0 7.965519063841589 7.879223384953156E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12965 0.0 50.131824 1 TACCTGG 8290 0.0 42.104218 2 GTATCAA 22715 0.0 40.720222 1 ACCTGGG 9820 0.0 34.497967 3 GTACCTG 10185 0.0 34.326653 1 CAACGCA 28180 0.0 32.02873 5 ATCAACG 28250 0.0 31.873096 3 TCAACGC 29010 0.0 31.02192 4 TATCAAC 29830 0.0 30.573856 2 AACGCAG 30385 0.0 30.0441 6 GTACATG 29165 0.0 27.852957 1 TACATGG 30305 0.0 26.239925 2 ACGCAGA 35090 0.0 25.895353 7 CGCAGAG 36045 0.0 25.13118 8 ACATGGG 30555 0.0 25.092127 3 CCTGGGG 9555 0.0 23.620123 4 CATGGGG 20890 0.0 23.314531 4 TGGGGGG 12845 0.0 22.935125 6 GCAGAGT 39480 0.0 22.694397 9 TAACGCA 1035 0.0 22.667173 4 >>END_MODULE