FastQCFastQC Report
Mon 6 Jun 2016
ERR1211354_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211354_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9751028
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304150.31191583082317065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239410.2455228310286874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155620.15959342953378863No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139900.051.8080061
GTATCAA255400.041.8223271
ATCAACG318150.032.89133
CAACGCA319750.032.6537445
TCAACGC325800.032.1768464
TACCTGG82300.031.9462172
TATCAAC330850.031.9148392
AACGCAG338750.030.988386
GTACATG339050.029.7144451
TACATGG350300.028.3033542
ACGCAGA381800.027.3849147
ACATGGG353850.027.3818173
CGCAGAG393000.026.5202338
GTACCTG102700.026.4709381
CATGGGG252450.024.7554764
ACCTGGG104650.024.695413
GTGGTAT67050.024.5516761
GCAGAGT435550.023.4882749
ATGGGGG172450.022.7521635
GTACTAG31100.022.685211
Produced by FastQC (version 0.11.5)