##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211354_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9751028 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.346170885777376 31.0 31.0 34.0 28.0 34.0 2 31.353813054377447 31.0 31.0 34.0 28.0 34.0 3 31.406917814203794 31.0 31.0 34.0 28.0 34.0 4 35.00926107483232 37.0 35.0 37.0 32.0 37.0 5 35.05256358611626 37.0 35.0 37.0 32.0 37.0 6 35.118428128808574 37.0 35.0 37.0 32.0 37.0 7 35.05201010601139 37.0 35.0 37.0 32.0 37.0 8 35.05165045162418 37.0 35.0 37.0 32.0 37.0 9 36.60919884549609 39.0 37.0 39.0 32.0 39.0 10-11 36.53240545509663 39.0 37.0 39.0 32.0 39.0 12-13 36.44622869506682 39.0 37.0 39.0 32.0 39.0 14-15 37.57827820820533 40.0 37.0 41.0 32.0 41.0 16-17 37.57291118433872 40.0 37.0 41.0 32.0 41.0 18-19 37.5679260689232 40.0 37.0 41.0 32.0 41.0 20-21 37.52874922520989 40.0 37.0 41.0 31.5 41.0 22-23 37.405601850389516 40.0 37.0 41.0 31.0 41.0 24-25 37.294813736561935 40.0 37.0 41.0 31.0 41.0 26-27 37.15356206545607 40.0 36.0 41.0 30.5 41.0 28-29 37.00989890501802 39.5 36.0 41.0 30.0 41.0 30-31 36.82455054995227 39.0 36.0 41.0 30.0 41.0 32-33 36.697312990999514 39.0 35.5 41.0 30.0 41.0 34-35 36.54550120253988 39.0 35.0 41.0 30.0 41.0 36-37 36.4460820951391 39.0 35.0 41.0 29.5 41.0 38-39 36.294317635022686 39.0 35.0 40.5 29.0 41.0 40-41 36.12924242449104 39.0 35.0 40.0 28.0 41.0 42-43 35.984213100403366 39.0 35.0 40.0 27.5 41.0 44-45 35.852627025581306 38.5 35.0 40.0 27.0 41.0 46-47 35.69392427136913 38.5 34.5 40.0 26.5 41.0 48-49 35.46673678918776 38.0 34.0 40.0 26.0 41.0 50-51 34.80614618274093 37.5 33.5 39.5 24.5 40.5 52-53 35.05787394929027 38.0 33.5 40.0 26.0 40.5 54-55 35.42251278531864 38.0 34.0 40.0 26.0 41.0 56-57 35.32948120957093 38.0 34.0 40.0 26.0 41.0 58-59 35.11177031796032 38.0 34.0 40.0 26.0 41.0 60-61 34.84448675565284 37.5 34.0 40.0 25.5 41.0 62-63 34.571839399907375 37.0 33.0 40.0 25.0 41.0 64-65 34.29307530447046 36.5 33.0 40.0 25.0 41.0 66-67 34.06837904680409 36.0 33.0 39.0 24.5 41.0 68-69 33.72033733263816 36.0 32.5 39.0 23.5 41.0 70-71 33.36927409089585 35.0 32.0 39.0 23.0 40.5 72-73 32.99635320501592 35.0 32.0 38.0 22.5 40.0 74-75 32.62323705767228 35.0 31.5 37.0 22.0 39.5 76-77 32.265202140738396 35.0 31.0 37.0 21.5 39.0 78-79 31.892810942600104 35.0 31.0 36.0 20.0 39.0 80-81 31.552885449616184 35.0 31.0 36.0 20.0 37.5 82-83 31.236715759610163 34.0 30.0 35.0 20.0 37.0 84-85 30.932948967021733 34.0 30.0 35.0 19.0 37.0 86-87 30.673606772537212 34.0 30.0 35.0 18.5 36.0 88-89 30.41314228612614 34.0 29.0 35.0 18.0 36.0 90-91 30.148067772956864 34.0 29.0 35.0 17.5 35.5 92-93 29.909908627069882 34.0 29.0 35.0 17.0 35.0 94-95 29.683516343097363 34.0 29.0 35.0 15.0 35.0 96-97 29.472537254533574 34.0 28.5 35.0 15.0 35.0 98-99 29.231154756195963 34.0 27.0 35.0 12.0 35.0 100 27.55831446694646 32.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.40110382142473 1101 2 -0.4051772476088935 1101 3 -0.31910507225003926 1101 4 -0.25891464439865786 1101 5 -0.3175774599196757 1101 6 -0.3273278492092899 1101 7 -0.4285630245651646 1101 8 -0.5258469707349676 1101 9 -0.4472673011492958 1101 10-11 -0.5115720373760482 1101 12-13 -0.5118057110308172 1101 14-15 -0.5891283123486559 1101 16-17 -0.5607281095499701 1101 18-19 -0.5962700612915626 1101 20-21 -0.7305336737735288 1101 22-23 -0.6453619579528223 1101 24-25 -0.6795619569127709 1101 26-27 -0.7709551262135577 1101 28-29 -0.8070909210752362 1101 30-31 -0.8604623382657977 1101 32-33 -0.7566917347198583 1101 34-35 -0.7896557279601737 1101 36-37 -0.8551957331936251 1101 38-39 -0.8832232892687912 1101 40-41 -0.8811288773617676 1101 42-43 -0.9399535370623369 1101 44-45 -1.0495448297479513 1101 46-47 -1.0409490083431265 1101 48-49 -1.0224421860576882 1101 50-51 -1.0367517889407338 1101 52-53 -0.776434399885936 1101 54-55 -0.6780823199520114 1101 56-57 -0.6518741059798359 1101 58-59 -0.7466966117551621 1101 60-61 -0.7690624474386212 1101 62-63 -0.7582511215587715 1101 64-65 -0.785037832593865 1101 66-67 -0.9190554513839402 1101 68-69 -0.8763218408471047 1101 70-71 -0.8435614577532107 1101 72-73 -0.8192146611412525 1101 74-75 -0.8749829460692169 1101 76-77 -0.9258810518046303 1101 78-79 -0.8809147014889618 1101 80-81 -0.9946239345259791 1101 82-83 -1.1044927004501695 1101 84-85 -1.0760916749835872 1101 86-87 -1.034026562295086 1101 88-89 -1.0483672742241517 1101 90-91 -1.0498486525699562 1101 92-93 -1.0434756753019698 1101 94-95 -1.1583096085435756 1101 96-97 -1.231608338691231 1101 98-99 -1.2366044427238165 1101 100 -1.2143056891703345 1102 1 -0.4603000952432943 1102 2 -0.46297507450953646 1102 3 -0.4557434773255693 1102 4 -0.31788137036630104 1102 5 -0.3024036273795545 1102 6 -0.26886890523747553 1102 7 -0.3762775294212801 1102 8 -0.3650877973276607 1102 9 -0.48307090748754433 1102 10-11 -0.42775320538913775 1102 12-13 -0.4964077342833946 1102 14-15 -0.6215162407800179 1102 16-17 -0.6249972276945925 1102 18-19 -0.6114366711397139 1102 20-21 -0.6682095998242872 1102 22-23 -0.683545013282064 1102 24-25 -0.710608858827328 1102 26-27 -0.7159829234123478 1102 28-29 -0.7602204386015501 1102 30-31 -0.7453774491646215 1102 32-33 -0.7840616711580282 1102 34-35 -0.7808180420721484 1102 36-37 -0.8201633231022285 1102 38-39 -0.9864323339837782 1102 40-41 -0.9301300673403929 1102 42-43 -0.7886344621789618 1102 44-45 -0.8422782612670972 1102 46-47 -0.9711647228663836 1102 48-49 -0.9201561478743585 1102 50-51 -0.8341627427790641 1102 52-53 -0.7313212786193972 1102 54-55 -0.6866727757691535 1102 56-57 -0.6926289077859522 1102 58-59 -0.7373617906542833 1102 60-61 -0.8581143203842956 1102 62-63 -0.8463993554875344 1102 64-65 -0.9046259230061295 1102 66-67 -0.7849403873737657 1102 68-69 -0.7889605325832534 1102 70-71 -0.9109015307676671 1102 72-73 -0.9076272322205696 1102 74-75 -0.8684791928374622 1102 76-77 -0.8292109642570438 1102 78-79 -0.8933358992445548 1102 80-81 -0.8530814018585424 1102 82-83 -0.9150447404272875 1102 84-85 -0.8864027044301288 1102 86-87 -0.9267032716066659 1102 88-89 -0.9305346457185486 1102 90-91 -0.9824560411591321 1102 92-93 -1.0482257589246942 1102 94-95 -1.0722886446533941 1102 96-97 -1.1230867231537367 1102 98-99 -1.1187434049631548 1102 100 -1.1674075197107854 1103 1 -0.4337177741398861 1103 2 -0.49151196599809666 1103 3 -0.4453909648358554 1103 4 -0.32223099184784587 1103 5 -0.3074456858078207 1103 6 -0.42110724288557577 1103 7 -0.38705646191550613 1103 8 -0.44180259037520386 1103 9 -0.5652477036423917 1103 10-11 -0.5805230879684871 1103 12-13 -0.6218443297993588 1103 14-15 -0.7097866617893018 1103 16-17 -0.7013658683435224 1103 18-19 -0.6727454623375877 1103 20-21 -0.796335186568065 1103 22-23 -0.8205462229257208 1103 24-25 -0.7394157185281145 1103 26-27 -0.7165420479441167 1103 28-29 -0.7906206644609028 1103 30-31 -0.8272899595357117 1103 32-33 -0.7965401387186049 1103 34-35 -0.8694358180404791 1103 36-37 -0.8984025531294719 1103 38-39 -0.8744737716265547 1103 40-41 -0.810864747724672 1103 42-43 -0.9161539188140466 1103 44-45 -0.9296345116846894 1103 46-47 -0.9035078925979363 1103 48-49 -0.9709911397720106 1103 50-51 -0.9053581798365755 1103 52-53 -0.8058530015824559 1103 54-55 -0.81533393800391 1103 56-57 -0.7522102275133165 1103 58-59 -0.6871528834397296 1103 60-61 -0.6465087980575888 1103 62-63 -0.7550952312813806 1103 64-65 -0.759565681863954 1103 66-67 -0.8116846048288409 1103 68-69 -0.7687847531337866 1103 70-71 -0.7897073438444195 1103 72-73 -0.873760764638007 1103 74-75 -0.8217779694291139 1103 76-77 -0.8406595246944022 1103 78-79 -0.8554320530175197 1103 80-81 -0.8707526839962476 1103 82-83 -0.9791089676212401 1103 84-85 -0.865871601406802 1103 86-87 -1.002978495774503 1103 88-89 -1.0332016446266792 1103 90-91 -0.9762704666830544 1103 92-93 -1.0330498029235002 1103 94-95 -1.1470662581899056 1103 96-97 -1.2330280101883702 1103 98-99 -1.259454526791881 1103 100 -1.2222460731960219 1104 1 -0.3629483581195352 1104 2 -0.339455226884251 1104 3 -0.3655345799461607 1104 4 -0.2591833211757333 1104 5 -0.24374976291698403 1104 6 -0.28466969921634444 1104 7 -0.3005129129652744 1104 8 -0.27836380994694565 1104 9 -0.40525349528412846 1104 10-11 -0.39926966585036894 1104 12-13 -0.4351371622263116 1104 14-15 -0.5665203518978785 1104 16-17 -0.5414750878064751 1104 18-19 -0.6289939324649936 1104 20-21 -0.5373512699698111 1104 22-23 -0.5148100914514515 1104 24-25 -0.6065428109616207 1104 26-27 -0.712908738877907 1104 28-29 -0.6443865466732035 1104 30-31 -0.6845925817241749 1104 32-33 -0.7305210561867597 1104 34-35 -0.5905121006772376 1104 36-37 -0.7064805163332579 1104 38-39 -0.7032353159055234 1104 40-41 -0.7860210793574112 1104 42-43 -0.7655911312612531 1104 44-45 -0.8200091976457244 1104 46-47 -0.8138197438845154 1104 48-49 -0.7574487615366934 1104 50-51 -0.7011225595321378 1104 52-53 -0.5727479144358512 1104 54-55 -0.6479973228632474 1104 56-57 -0.6094992969544037 1104 58-59 -0.6559537210667159 1104 60-61 -0.6438193979848066 1104 62-63 -0.6853134989037173 1104 64-65 -0.690519302978835 1104 66-67 -0.6559982001438271 1104 68-69 -0.5950146745112477 1104 70-71 -0.6620715551986507 1104 72-73 -0.656720986948919 1104 74-75 -0.7127135559682998 1104 76-77 -0.6600756952659452 1104 78-79 -0.7065584174372326 1104 80-81 -0.6331210714678299 1104 82-83 -0.7624016044985886 1104 84-85 -0.8086203623812978 1104 86-87 -0.8342207857963935 1104 88-89 -0.7498733766079688 1104 90-91 -0.7363868777331781 1104 92-93 -0.849676275185395 1104 94-95 -0.8204554433373268 1104 96-97 -0.8927992406033631 1104 98-99 -0.8954066620460068 1104 100 -1.0291545403780553 1105 1 -0.35212162683107096 1105 2 -0.36286437083994016 1105 3 -0.3341673989591385 1105 4 -0.22748487193000244 1105 5 -0.20384823355554005 1105 6 -0.19708800887903521 1105 7 -0.2073908228519059 1105 8 -0.27255552012917406 1105 9 -0.3897780014417833 1105 10-11 -0.3922651169201785 1105 12-13 -0.39991614132907927 1105 14-15 -0.41167211962818584 1105 16-17 -0.5168053156450441 1105 18-19 -0.4956892070565715 1105 20-21 -0.4808410503049032 1105 22-23 -0.5403351539766916 1105 24-25 -0.5283664682235312 1105 26-27 -0.4456304844086745 1105 28-29 -0.5869890193476479 1105 30-31 -0.5981264666587904 1105 32-33 -0.5532250761391921 1105 34-35 -0.6235327008193465 1105 36-37 -0.6001473940376485 1105 38-39 -0.6468340863251925 1105 40-41 -0.6179088566681941 1105 42-43 -0.7048223965321867 1105 44-45 -0.7012702406310396 1105 46-47 -0.6951511454003096 1105 48-49 -0.663539713658146 1105 50-51 -0.5831112528632758 1105 52-53 -0.4236027686878501 1105 54-55 -0.41328147343792665 1105 56-57 -0.40394545534140747 1105 58-59 -0.4284455880863689 1105 60-61 -0.5017112665671419 1105 62-63 -0.47549431969593314 1105 64-65 -0.4233729486646638 1105 66-67 -0.4888021025758036 1105 68-69 -0.4603224824160037 1105 70-71 -0.5099421360969316 1105 72-73 -0.4867367401153402 1105 74-75 -0.4664009255000252 1105 76-77 -0.549400533974314 1105 78-79 -0.4000711425550243 1105 80-81 -0.41846338635663827 1105 82-83 -0.45142566616931745 1105 84-85 -0.5070470830314093 1105 86-87 -0.5661321712089986 1105 88-89 -0.5437827072333938 1105 90-91 -0.5668043965377052 1105 92-93 -0.5871050871904409 1105 94-95 -0.6366602486633859 1105 96-97 -0.688241419037773 1105 98-99 -0.7005492735741541 1105 100 -0.7649440453265228 1106 1 -0.5086156099904748 1106 2 -0.5143593990514503 1106 3 -0.5075268902443675 1106 4 -0.3841238558338702 1106 5 -0.4080916283185445 1106 6 -0.445078227397147 1106 7 -0.5029621702780673 1106 8 -0.42999350796213776 1106 9 -0.5628413287378322 1106 10-11 -0.6236652195339616 1106 12-13 -0.6208212863553655 1106 14-15 -0.7606700788867329 1106 16-17 -0.8058054610223166 1106 18-19 -0.8039069337885465 1106 20-21 -0.6984421036682278 1106 22-23 -0.7491855197223387 1106 24-25 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0.8896554614556429 2204 96-97 0.8907995367269059 2204 98-99 0.8794540400441022 2204 100 0.9118023149479342 2205 1 0.7370156206940592 2205 2 0.752122720348769 2205 3 0.7548165997397867 2205 4 0.5892081225432975 2205 5 0.5676696278453193 2205 6 0.566531133498188 2205 7 0.6181326524756372 2205 8 0.5840276679273799 2205 9 0.7574460232948752 2205 10-11 0.7673205467178548 2205 12-13 0.774396445108394 2205 14-15 0.7458406444837706 2205 16-17 0.8999948327634897 2205 18-19 0.9148121944889738 2205 20-21 0.9233257912917097 2205 22-23 0.8583513785502888 2205 24-25 0.8870026394054662 2205 26-27 0.9699662546389121 2205 28-29 0.9750850550962866 2205 30-31 1.0186855939698276 2205 32-33 0.9986512819456053 2205 34-35 0.9787998830665288 2205 36-37 1.017268572331787 2205 38-39 0.9778094387055489 2205 40-41 1.064527318695987 2205 42-43 1.0567243099682315 2205 44-45 0.9962072779832098 2205 46-47 1.0242019782507796 2205 48-49 1.0245923473128542 2205 50-51 1.010324476989993 2205 52-53 0.9996474596721541 2205 54-55 0.9498528701141709 2205 56-57 0.9730895879848376 2205 58-59 0.9956192567816728 2205 60-61 0.9858482130125239 2205 62-63 1.0027230344012779 2205 64-65 1.056578229575159 2205 66-67 0.9758961147283571 2205 68-69 0.8832332357960979 2205 70-71 0.8737497594766097 2205 72-73 0.9495210619302625 2205 74-75 0.9960379899804863 2205 76-77 0.9255535749365933 2205 78-79 0.9441412864658751 2205 80-81 0.9098400281808772 2205 82-83 0.9416474899433318 2205 84-85 0.8995688967481783 2205 86-87 0.9081262090225444 2205 88-89 0.8584035214249788 2205 90-91 0.8938171716933283 2205 92-93 0.937902687851409 2205 94-95 1.028971564688323 2205 96-97 1.018505661398347 2205 98-99 1.023502115274539 2205 100 1.0536764360290682 2206 1 0.6850910697699995 2206 2 0.6971122419664191 2206 3 0.7277407093977821 2206 4 0.5873868035895669 2206 5 0.562026027114193 2206 6 0.5892648712579458 2206 7 0.59389523648656 2206 8 0.6473810606494581 2206 9 0.7817202620603041 2206 10-11 0.8053549071080894 2206 12-13 0.8319903372842816 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0.9885541694089603 2206 82-83 0.9881973239962569 2206 84-85 1.0080543622940041 2206 86-87 1.0047415413991274 2206 88-89 0.9954810294052159 2206 90-91 0.9990651257773671 2206 92-93 1.0129113748554488 2206 94-95 1.0476497956971862 2206 96-97 1.0994297174162 2206 98-99 1.1042102612890758 2206 100 1.2641639945524865 2207 1 0.6866494408413573 2207 2 0.6909237992217214 2207 3 0.6633480973894841 2207 4 0.5248895462385335 2207 5 0.5316315317733569 2207 6 0.5662092160424592 2207 7 0.5618004395429281 2207 8 0.5905813098086696 2207 9 0.7477643883966607 2207 10-11 0.7947224208769725 2207 12-13 0.7805947847588754 2207 14-15 0.9915087861180893 2207 16-17 0.9936283912463324 2207 18-19 0.9384290655970062 2207 20-21 0.9648989761324245 2207 22-23 0.9936094188004958 2207 24-25 0.9880905686872197 2207 26-27 1.0500176902510745 2207 28-29 0.9872586094753757 2207 30-31 1.0186023241867517 2207 32-33 0.973111168751501 2207 34-35 0.9987804036016783 2207 36-37 1.0192113139336243 2207 38-39 1.0645075524490508 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2311 18-19 0.751566498831437 2311 20-21 0.7939789594337086 2311 22-23 0.8866641518015044 2311 24-25 0.8678944493260232 2311 26-27 0.9295824401906359 2311 28-29 0.9918651767501387 2311 30-31 1.000413341177584 2311 32-33 0.9805486336471816 2311 34-35 0.9934562956700219 2311 36-37 0.9383877745130533 2311 38-39 1.0338290909347876 2311 40-41 1.0570790928763572 2311 42-43 1.0783152083592213 2311 44-45 1.1180941645477773 2311 46-47 1.091512839058737 2311 48-49 1.0808106000654192 2311 50-51 1.0652075084025512 2311 52-53 0.9892250474648066 2311 54-55 0.9917548272158498 2311 56-57 0.9208983070043146 2311 58-59 0.963867889874038 2311 60-61 0.9722659355808005 2311 62-63 0.9722983850691165 2311 64-65 0.9959186157225801 2311 66-67 0.9776306935318217 2311 68-69 0.9518461688155213 2311 70-71 0.8775463981902902 2311 72-73 0.8569574800017179 2311 74-75 0.9425100836770852 2311 76-77 0.9538760835900106 2311 78-79 0.9462556552469472 2311 80-81 0.9953585753831184 2311 82-83 0.9577296891891827 2311 84-85 1.0518561109535938 2311 86-87 1.0535011657681004 2311 88-89 1.048159688272623 2311 90-91 1.0708448130054649 2311 92-93 1.0563424643844144 2311 94-95 1.1505215205847676 2311 96-97 1.1180441335555997 2311 98-99 1.1572390040081402 2311 100 1.281195576435092 2312 1 0.5151132920548704 2312 2 0.5398234873348002 2312 3 0.507266942609462 2312 4 0.448042626833697 2312 5 0.4873975595714839 2312 6 0.4942000722108375 2312 7 0.5236348864268408 2312 8 0.5589934075766649 2312 9 0.6432409199914773 2312 10-11 0.6384846808301177 2312 12-13 0.6059646592216978 2312 14-15 0.8390328200152055 2312 16-17 0.827491500717727 2312 18-19 0.7355307818479702 2312 20-21 0.7639301292393341 2312 22-23 0.7809301349369875 2312 24-25 0.8555182504474246 2312 26-27 0.8734451774116394 2312 28-29 0.8498383414782182 2312 30-31 0.8810064902718366 2312 32-33 0.8988441970814094 2312 34-35 0.880776578043104 2312 36-37 0.9304991686964428 2312 38-39 0.9513750911527126 2312 40-41 0.8804323267790721 2312 42-43 0.9655443374097885 2312 44-45 0.9283487395452426 2312 46-47 0.9561990123474331 2312 48-49 0.972922128183022 2312 50-51 0.9664140567862489 2312 52-53 0.8578684466696913 2312 54-55 0.7871586948562168 2312 56-57 0.7665271130417182 2312 58-59 0.753507455084808 2312 60-61 0.8082903605135172 2312 62-63 0.7893824954981881 2312 64-65 0.752222802432847 2312 66-67 0.7360670430200003 2312 68-69 0.8073726472267282 2312 70-71 0.7392518902891041 2312 72-73 0.7419336959565186 2312 74-75 0.7737299269620621 2312 76-77 0.8156198989498478 2312 78-79 0.8274944781598101 2312 80-81 0.8462398250974239 2312 82-83 0.8227242439418738 2312 84-85 0.875899327891279 2312 86-87 0.9292634106231255 2312 88-89 0.9013881802071921 2312 90-91 0.9258685615539974 2312 92-93 0.9835493692303139 2312 94-95 1.0154902580805611 2312 96-97 1.0819487900479174 2312 98-99 1.1420190696150314 2312 100 1.2520120434046191 2313 1 0.3656735211680342 2313 2 0.37233689590409114 2313 3 0.4359023749301443 2313 4 0.3408187792406707 2313 5 0.37032681776513954 2313 6 0.35680760365895026 2313 7 0.4206926660407646 2313 8 0.4120830911511959 2313 9 0.5239259945497281 2313 10-11 0.5038941213704504 2313 12-13 0.5343756936973776 2313 14-15 0.646706410307722 2313 16-17 0.6389263167719861 2313 18-19 0.6447889399894748 2313 20-21 0.6038296791533426 2313 22-23 0.5650659739785269 2313 24-25 0.5898434026052186 2313 26-27 0.6534525522480621 2313 28-29 0.663780562456175 2313 30-31 0.7344708976105991 2313 32-33 0.7800960686582457 2313 34-35 0.7238955656426143 2313 36-37 0.8063204064496858 2313 38-39 0.7976374321167725 2313 40-41 0.8181126901624012 2313 42-43 0.7113699412713785 2313 44-45 0.8691523836377613 2313 46-47 0.8569876936899874 2313 48-49 0.9008520265945563 2313 50-51 0.8399057143231516 2313 52-53 0.7663336934929177 2313 54-55 0.7118079235268482 2313 56-57 0.6878981420878816 2313 58-59 0.6565307039705459 2313 60-61 0.7023017605348656 2313 62-63 0.7314724389084475 2313 64-65 0.7231371894207754 2313 66-67 0.7298077758909187 2313 68-69 0.694464694773707 2313 70-71 0.7162827235371054 2313 72-73 0.7005576635435418 2313 74-75 0.6720919517235941 2313 76-77 0.6941983745684013 2313 78-79 0.7087045984556717 2313 80-81 0.7036707225053185 2313 82-83 0.700184818259725 2313 84-85 0.7806158336945757 2313 86-87 0.818032363390433 2313 88-89 0.8570200200079121 2313 90-91 0.8812656968897414 2313 92-93 0.8980749164883548 2313 94-95 0.9811994547462213 2313 96-97 1.0238552661526015 2313 98-99 1.0199319238286435 2313 100 1.08189512521815 2314 1 0.365412533628799 2314 2 0.31499121167031063 2314 3 0.3321724208853105 2314 4 0.268097729021342 2314 5 0.2859602894909372 2314 6 0.2879453328441244 2314 7 0.3272995888530801 2314 8 0.37052019161028227 2314 9 0.4168523136387705 2314 10-11 0.43718109313057596 2314 12-13 0.47602367825622594 2314 14-15 0.5735333376663618 2314 16-17 0.5982196071000061 2314 18-19 0.5399207920159057 2314 20-21 0.5677876453344055 2314 22-23 0.6178161649803471 2314 24-25 0.6818958093449012 2314 26-27 0.6643506098727627 2314 28-29 0.6419882351584789 2314 30-31 0.6699571409094958 2314 32-33 0.7008089230568046 2314 34-35 0.7330205779660943 2314 36-37 0.7892359042085104 2314 38-39 0.7983813329408846 2314 40-41 0.7753832142784134 2314 42-43 0.7155549317655243 2314 44-45 0.7873479963418717 2314 46-47 0.6181208979451682 2314 48-49 0.5260496913651025 2314 50-51 0.6892903607903946 2314 52-53 0.6298908912720336 2314 54-55 0.6507940008597899 2314 56-57 0.6321348921808791 2314 58-59 0.6486682346188317 2314 60-61 0.41536520036448366 2314 62-63 0.5822497526556631 2314 64-65 0.6093900709767084 2314 66-67 0.6143105654217891 2314 68-69 0.6091589217058555 2314 70-71 0.6377400426745652 2314 72-73 0.6687710823430209 2314 74-75 0.6998370179067805 2314 76-77 0.6504979891034779 2314 78-79 0.6501301322447581 2314 80-81 0.6912985683061343 2314 82-83 0.730942173312723 2314 84-85 0.7337923248262364 2314 86-87 0.7807438717827502 2314 88-89 0.6722237925589098 2314 90-91 0.6686862553948316 2314 92-93 0.873937244278018 2314 94-95 0.8744340252113574 2314 96-97 0.8245078205601679 2314 98-99 0.8805774118006298 2314 100 0.9428263896453757 2315 1 0.25280337996212765 2315 2 0.25045243561802266 2315 3 0.30726547772448853 2315 4 0.18888553161232124 2315 5 0.1804785701070344 2315 6 0.1859462340006246 2315 7 0.1378033266365435 2315 8 0.18170568655334307 2315 9 0.11145845689671319 2315 10-11 0.27501553533763 2315 12-13 0.22632362263966854 2315 14-15 0.4124397206311201 2315 16-17 0.26396743232148623 2315 18-19 0.3942135564910174 2315 20-21 0.30062116322341836 2315 22-23 0.26104988596127754 2315 24-25 0.4356318484802557 2315 26-27 0.4797589866679104 2315 28-29 0.3755566079191084 2315 30-31 0.435896509247236 2315 32-33 0.4299313895422756 2315 34-35 0.3331543224570481 2315 36-37 0.5085747099194293 2315 38-39 0.5706314161949919 2315 40-41 0.6601578386580869 2315 42-43 0.6248239142579877 2315 44-45 0.6612434054806329 2315 46-47 0.66848422981748 2315 48-49 0.728947454367308 2315 50-51 0.5966985690075148 2315 52-53 0.26620355040194 2315 54-55 0.493154853456403 2315 56-57 0.4761241083949628 2315 58-59 0.5277600317379765 2315 60-61 0.5516468046220027 2315 62-63 0.5534318952160859 2315 64-65 0.511714163522889 2315 66-67 0.37567280340014975 2315 68-69 0.5302573120001028 2315 70-71 0.4269306075409034 2315 72-73 0.5568297078301256 2315 74-75 0.28079671161366804 2315 76-77 0.5010114103005421 2315 78-79 0.593701854347632 2315 80-81 0.6226309637488008 2315 82-83 0.5731091697566413 2315 84-85 0.5640303679266268 2315 86-87 0.5461415241809604 2315 88-89 0.46868867380935697 2315 90-91 0.6533239007745664 2315 92-93 0.7374360029055893 2315 94-95 0.8046161274437189 2315 96-97 0.8029450899557169 2315 98-99 0.9107301454085999 2315 100 0.888976094929685 2316 1 0.07189819989661927 2316 2 0.14228147947168068 2316 3 0.0700736761724059 2316 4 -0.00959055578885426 2316 5 -0.010681624662495892 2316 6 -0.15024804292779947 2316 7 -0.13371944610814523 2316 8 -0.045638220568235965 2316 9 0.05910832602465632 2316 10-11 0.0010830246487572026 2316 12-13 0.053007393698251803 2316 14-15 -0.10237399523720114 2316 16-17 0.07132984183571978 2316 18-19 0.12334983795018672 2316 20-21 0.20763903120590754 2316 22-23 0.08725416541960129 2316 24-25 0.0771676580101257 2316 26-27 -0.011868158092973147 2316 28-29 0.04137538852552325 2316 30-31 -0.5874191466301752 2316 32-33 -0.4247717872536967 2316 34-35 -0.4996602425865362 2316 36-37 -0.25844421207235513 2316 38-39 -0.15950239065293914 2316 40-41 0.08320621564979547 2316 42-43 0.10325284805581703 2316 44-45 -0.021837593007099088 2316 46-47 -0.08159731894941302 2316 48-49 -0.039080251540291044 2316 50-51 0.0861422404659038 2316 52-53 -0.2658724828114103 2316 54-55 -0.13922831681230008 2316 56-57 -0.2031375600794192 2316 58-59 -0.573594037392084 2316 60-61 -0.10706623869871379 2316 62-63 -0.14668790652917352 2316 64-65 -0.3862398518631096 2316 66-67 -0.654154619256218 2316 68-69 -0.6383208712284869 2316 70-71 -0.43981149623013494 2316 72-73 -0.6543576927128569 2316 74-75 -0.4776438822385529 2316 76-77 -0.14049125486021552 2316 78-79 -0.056899839133766505 2316 80-81 -0.11849527793513559 2316 82-83 0.08301884262860071 2316 84-85 0.10681995120534893 2316 86-87 0.17910869180655808 2316 88-89 0.14955933231589924 2316 90-91 -0.4458262743879118 2316 92-93 -0.48621495049922814 2316 94-95 -0.048179017845757954 2316 96-97 0.13530637591255612 2316 98-99 0.22849313476871913 2316 100 0.16327098252398642 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 285.0 1 720.0 2 431.0 3 378.0 4 412.0 5 376.0 6 414.0 7 1044.0 8 2547.0 9 4585.0 10 7522.0 11 12451.0 12 19170.0 13 24695.0 14 28633.0 15 33029.0 16 39028.0 17 46080.0 18 55721.0 19 66005.0 20 76957.0 21 91232.0 22 102493.0 23 106548.0 24 108761.0 25 113962.0 26 124480.0 27 141527.0 28 165553.0 29 196237.0 30 235482.0 31 289831.0 32 363391.0 33 468262.0 34 639599.0 35 883984.0 36 1295947.0 37 1875091.0 38 1730202.0 39 397584.0 40 379.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.97908722352144 18.347224920619258 12.689922415414912 23.983765440444387 2 16.00827978715748 19.78959271163543 40.4223586695628 23.779768831644294 3 19.347483723556998 25.14366575221404 29.29792562921528 26.210924895013687 4 12.832088102847653 15.916770921662026 37.399820069742205 33.85132090574812 5 13.459736718423207 37.25973437974723 34.431598138331864 14.8489307634977 6 31.681262701227926 35.992849606893564 18.05790267105534 14.267985020823165 7 28.067012980084073 32.017099524238006 21.84524243136233 18.070645064315592 8 26.165364644114238 35.35547539408723 20.066272908591543 18.412887053206994 9 26.797567327762913 15.940675428371062 20.2403640042229 37.021393239643125 10-11 25.34406167436765 25.80542835138574 28.09883771688871 20.751672257357896 12-13 25.62619444437463 23.46198190987273 28.273009056758973 22.638814588993668 14-15 23.030050107877432 25.04846572355792 25.952668524406132 25.968815644158518 16-17 22.54092093645161 27.85705063324839 26.698363839656587 22.903664590643412 18-19 22.248239523317082 27.104949442720898 28.647038787938477 21.99977224602354 20-21 23.11483332136298 26.45501369750572 28.502452212634537 21.927700768496763 22-23 22.966458355061494 26.191851915427232 28.315538600422425 22.52615112908885 24-25 22.582015659893038 26.229617884365876 28.58987998474218 22.598486470998903 26-27 22.497194523183868 26.437627293123278 28.371826034989038 22.693352148703816 28-29 22.4162445996343 26.735141712480836 28.405378482325567 22.443235205559294 30-31 22.96068559914588 26.45201908464168 28.383774473673558 22.203520842538882 32-33 22.749019439259506 26.594657135390893 28.22086591395525 22.435457511394347 34-35 22.890949304560976 26.644554288136234 28.289044451195533 22.175451956107256 36-37 22.79644916026614 26.550422222772816 28.155734149826824 22.497394467134225 38-39 22.764602509361268 26.709667549851517 28.196620258421856 22.32910968236536 40-41 22.821566396097477 26.39687012396609 28.36147714330525 22.420086336631183 42-43 22.626608540138154 26.48510908318247 28.093545327461438 22.794737049217943 44-45 22.45863799926056 26.785479922140425 28.141255056187966 22.614627022411053 46-47 22.443915493278602 26.589898626700613 27.907636588794478 23.058549291226303 48-49 22.659872818003734 26.64303254254194 27.860101856422055 22.836992783032276 50-51 22.59556423047466 26.826051203456906 27.776162802629127 22.802221763439306 52-53 22.529507273480316 26.972920503788927 27.612287520744577 22.885284701986183 54-55 22.401490290548377 26.810782946349683 27.82359648590876 22.964130277193178 56-57 22.638430833502728 26.863555035617587 27.512262704621683 22.985751426258005 58-59 22.425886833066404 26.8753318420567 27.802732431552357 22.896048893324537 60-61 22.668223515149773 26.953874772539205 27.64423720666086 22.733664505650157 62-63 22.623310632280916 26.891943058906104 27.628420677830096 22.856325630982884 64-65 22.394523543289655 26.698608901807386 27.685314087957096 23.221553466945867 66-67 22.463756851423266 26.668206805206285 27.584640540027365 23.283395803343083 68-69 22.260616779054118 26.75710508803983 27.703930251541642 23.27834788136441 70-71 22.313518498860454 26.521237150173626 27.755378699090993 23.409865651874927 72-73 22.36412866828263 26.75999552389261 27.545130619576074 23.330745188248688 74-75 22.405037210845244 26.79545111512486 27.443484467238644 23.356027206791254 76-77 22.250674104743034 26.64428047559335 27.5015342366407 23.603511183022913 78-79 22.215240766828558 26.58881642678646 27.620021172178493 23.57592163420649 80-81 22.221551785752858 26.767819111041263 27.473383372864284 23.537245730341596 82-83 22.48109370297667 26.603755638182374 27.66243439371538 23.252716265125574 84-85 22.51659628368845 26.68881693591568 27.33914888211226 23.455437898283616 86-87 22.156847765808184 26.78788626298249 27.61420254094167 23.441063430267658 88-89 22.239045719297117 26.43048412881012 27.673162409932516 23.657307741960246 90-91 22.51063371900689 26.372551902190466 27.63001774967605 23.486796629126598 92-93 22.466354221451102 26.460075691102997 27.518879799180628 23.554690288265274 94-95 22.2281082905195 26.32137871322644 27.64988536792186 23.800627628332197 96-97 22.242794909691536 26.211899889683366 27.661313058363135 23.883992142261963 98-99 22.185270999091234 26.253777889357906 27.547590166926106 24.013360944624758 100 22.406295713884656 26.118784272257034 27.36354710274474 24.11137291111357 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2662.0 1 2008.5 2 3077.5 3 4313.5 4 2933.5 5 2246.5 6 3151.0 7 5525.5 8 8596.0 9 10054.0 10 8818.5 11 7400.5 12 7539.5 13 8086.0 14 8767.5 15 9579.5 16 10335.0 17 11075.0 18 11977.5 19 12920.0 20 14057.0 21 15538.0 22 17934.5 23 21590.0 24 26166.0 25 31841.5 26 39386.5 27 48727.0 28 59340.5 29 72960.0 30 90311.0 31 107708.0 32 128201.0 33 151643.0 34 173535.0 35 194557.5 36 219906.0 37 247885.0 38 264813.0 39 273622.5 40 282180.5 41 289952.5 42 299356.5 43 315117.5 44 331867.0 45 351646.0 46 371069.5 47 384322.5 48 397808.0 49 408150.5 50 407954.0 51 401937.0 52 390177.5 53 373456.0 54 355615.5 55 327691.0 56 294650.0 57 259538.5 58 226518.5 59 198342.0 60 166642.0 61 134879.0 62 108459.0 63 85017.0 64 63207.5 65 45695.0 66 33600.5 67 25514.5 68 19839.0 69 15176.0 70 11597.0 71 8770.0 72 6699.0 73 5287.0 74 4061.5 75 2982.5 76 2117.0 77 1417.0 78 985.5 79 669.5 80 441.0 81 297.5 82 200.0 83 143.0 84 98.0 85 74.5 86 69.5 87 58.5 88 47.0 89 36.0 90 27.5 91 22.0 92 17.5 93 17.0 94 12.5 95 7.5 96 8.5 97 10.5 98 6.5 99 4.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025043513360847695 2 0.10953716879902303 3 0.017044356759102732 4 0.017382782615330404 5 0.01971074229301772 6 0.04544136269529735 7 0.046743789475325065 8 0.03852927096507158 9 0.03311445726542884 10-11 0.03666280109133108 12-13 0.03647307750526406 14-15 0.032401711901555406 16-17 0.03883180316988117 18-19 0.03761654668615453 20-21 0.048487195401346404 22-23 0.0477026627346368 24-25 0.06683397894047684 26-27 0.05952192938016381 28-29 0.051733007022439076 30-31 0.04990755846460496 32-33 0.04419021256015263 34-35 0.04564646927482928 36-37 0.04083159232031741 38-39 0.04598489513105695 40-41 0.05474807374155833 42-43 0.05647096900962647 44-45 0.05938861010346807 46-47 0.06109099471358302 48-49 0.0608961434630277 50-51 0.05574796831677645 52-53 0.06473163650027464 54-55 0.05136894284376991 56-57 0.0555941383821275 58-59 0.05373279617287532 60-61 0.04966655823365495 62-63 0.05658890529285733 64-65 0.05446092453021364 66-67 0.05283032722293486 68-69 0.058691247733059525 70-71 0.05857331144982868 72-73 0.06105510106216494 74-75 0.05208681587213163 76-77 0.0527021356107274 78-79 0.0632138478117384 80-81 0.06289080494897563 82-83 0.05333796600727637 84-85 0.05921426951086593 86-87 0.05688118216869032 88-89 0.06553155216044913 90-91 0.07082842957686103 92-93 0.07534077432656332 94-95 0.0787814371982113 96-97 0.07442292238315795 98-99 0.08362195247516467 100 0.12784293102224709 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9751028.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.628700753314554 #Duplication Level Percentage of deduplicated Percentage of total 1 74.66168746729787 43.77317732257702 2 14.712595100338868 17.25160670884899 3 4.990545238566707 8.77767550163419 4 2.1095897400295356 4.947300223218172 5 1.0730124487158959 3.145466288017277 6 0.6081835915604887 2.1394208275605586 7 0.4072646383329304 1.671417762575978 8 0.26908110284773806 1.2620700365785513 9 0.1996660734324762 1.0535546222875778 >10 0.8910828444894082 9.092027615055063 >50 0.05034895545812105 2.012812605434522 >100 0.0250487766367862 2.671650271573432 >500 0.0013355632530915218 0.5429819476518009 >1k 4.370549032729508E-4 0.4897308338785412 >5k 4.856165470257174E-5 0.17378739428908074 >10k+ 7.284248205385762E-5 0.9953200388191308 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30415 0.31191583082317065 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23941 0.2455228310286874 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15562 0.15959342953378863 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 1.0255328976596108E-5 9 0.0 0.0 0.0 0.0 2.0510657953192216E-5 10-11 0.0 0.0 0.0 0.0 2.0510657953192216E-5 12-13 0.0 0.0 0.0 5.127664488298054E-6 2.0510657953192216E-5 14-15 0.0 0.0 0.0 1.0255328976596108E-5 3.076598692978833E-5 16-17 0.0 0.0 0.0 3.589365141808638E-5 3.076598692978833E-5 18-19 0.0 0.0 0.0 4.102131590638443E-5 3.076598692978833E-5 20-21 0.0 0.0 0.0 1.0768095425425913E-4 3.076598692978833E-5 22-23 0.0 0.0 0.0 3.897025011106521E-4 4.102131590638444E-5 24-25 0.0 0.0 0.0 9.4861793033514E-4 5.127664488298054E-5 26-27 0.0 0.0 0.0 0.0020459381308309235 5.127664488298054E-5 28-29 0.0 0.0 0.0 0.005568643634291687 5.127664488298054E-5 30-31 0.0 0.0 0.0 0.019351805778836856 5.64043093712786E-5 32-33 0.0 0.0 0.0 0.05162532606818481 7.178730283617276E-5 34-35 5.127664488298054E-6 0.0 0.0 0.10205590631059618 7.178730283617276E-5 36-37 1.0255328976596108E-5 0.0 0.0 0.18015023646737555 7.178730283617276E-5 38-39 1.0255328976596108E-5 1.0255328976596108E-5 0.0 0.29666102897048396 7.691496732447081E-5 40-41 1.0255328976596108E-5 1.0255328976596108E-5 0.0 0.4229810436397065 8.204263181276886E-5 42-43 1.0255328976596108E-5 1.0255328976596108E-5 0.0 0.5466090344525726 8.717029630106693E-5 44-45 1.0255328976596108E-5 2.0510657953192216E-5 0.0 0.6960804542864609 9.229796078936498E-5 46-47 1.0255328976596108E-5 2.0510657953192216E-5 0.0 0.8683597257642989 9.742562527766303E-5 48-49 1.0255328976596108E-5 2.0510657953192216E-5 0.0 1.0524069872427808 1.2306394771915331E-4 50-51 1.0255328976596108E-5 2.0510657953192216E-5 0.0 1.2443252137108005 1.2306394771915331E-4 52-53 1.0255328976596108E-5 2.0510657953192216E-5 0.0 1.429808221246006 1.2819161220745137E-4 54-55 1.538299346489416E-5 2.0510657953192216E-5 0.0 1.6388528471049413 1.3331927669574942E-4 56-57 2.0510657953192216E-5 2.0510657953192216E-5 0.0 1.8786788428871293 1.3331927669574942E-4 58-59 2.0510657953192216E-5 2.0510657953192216E-5 0.0 2.151378295703797 1.3331927669574942E-4 60-61 2.0510657953192216E-5 2.0510657953192216E-5 0.0 2.42508277076017 1.3331927669574942E-4 62-63 2.0510657953192216E-5 2.0510657953192216E-5 0.0 2.703622633429009 1.3331927669574942E-4 64-65 2.0510657953192216E-5 2.0510657953192216E-5 0.0 2.9821676237623356 1.4357460567234552E-4 66-67 2.0510657953192216E-5 2.0510657953192216E-5 0.0 3.2944577740931518 1.4357460567234552E-4 68-69 2.0510657953192216E-5 2.0510657953192216E-5 0.0 3.6398316157024677 1.4357460567234552E-4 70-71 2.0510657953192216E-5 2.0510657953192216E-5 0.0 4.008074840929592 1.5382993464894162E-4 72-73 2.0510657953192216E-5 2.0510657953192216E-5 0.0 4.360965838678753 1.6408526362553773E-4 74-75 2.0510657953192216E-5 2.0510657953192216E-5 0.0 4.715107986563058 1.6408526362553773E-4 76-77 2.0510657953192216E-5 2.0510657953192216E-5 0.0 5.101267271512295 1.7434059260213386E-4 78-79 2.0510657953192216E-5 2.563832244149027E-5 0.0 5.5338575584030725 1.8459592157872996E-4 80-81 2.0510657953192216E-5 3.076598692978833E-5 0.0 5.970057721093612 1.89723586067028E-4 82-83 2.0510657953192216E-5 3.589365141808638E-5 0.0 6.407503906254807 1.9485125055532606E-4 84-85 2.0510657953192216E-5 4.102131590638443E-5 1.0255328976596108E-5 6.838668702417838 1.9485125055532606E-4 86-87 2.0510657953192216E-5 5.127664488298054E-5 1.0255328976596108E-5 7.2950103312184105 1.9485125055532606E-4 88 2.0510657953192216E-5 6.153197385957666E-5 1.0255328976596108E-5 7.654577548131336 2.0510657953192216E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13990 0.0 51.808006 1 GTATCAA 25540 0.0 41.822327 1 ATCAACG 31815 0.0 32.8913 3 CAACGCA 31975 0.0 32.653744 5 TCAACGC 32580 0.0 32.176846 4 TACCTGG 8230 0.0 31.946217 2 TATCAAC 33085 0.0 31.914839 2 AACGCAG 33875 0.0 30.98838 6 GTACATG 33905 0.0 29.714445 1 TACATGG 35030 0.0 28.303354 2 ACGCAGA 38180 0.0 27.384914 7 ACATGGG 35385 0.0 27.381817 3 CGCAGAG 39300 0.0 26.520233 8 GTACCTG 10270 0.0 26.470938 1 CATGGGG 25245 0.0 24.755476 4 ACCTGGG 10465 0.0 24.69541 3 GTGGTAT 6705 0.0 24.551676 1 GCAGAGT 43555 0.0 23.488274 9 ATGGGGG 17245 0.0 22.752163 5 GTACTAG 3110 0.0 22.68521 1 >>END_MODULE