FastQCFastQC Report
Mon 6 Jun 2016
ERR1211354_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211354_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9751028
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271960.27890392684750775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223810.22952451782519753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148750.1525480185268671No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140200.051.7184941
GTATCAA242600.040.180111
TACCTGG81050.032.7648742
CAACGCA305850.031.2277625
ATCAACG304950.031.173613
TCAACGC314450.030.3068834
TATCAAC322000.030.0308252
AACGCAG325000.029.604246
GTACATG332700.028.5934351
TACATGG338600.027.4915872
GTACCTG104400.026.5226521
ACATGGG342150.026.0974793
ACGCAGA375250.025.5149027
ACCTGGG103400.025.0038763
CGCAGAG391050.024.412028
CATGGGG238150.024.3135054
GTACTAG31850.022.8395581
GTGGTAT66800.022.7632351
ATGGGGG158200.022.2099195
GCAGAGT435200.021.622119
Produced by FastQC (version 0.11.5)