FastQCFastQC Report
Mon 6 Jun 2016
ERR1211353_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211353_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10015909
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312330.3118339034430125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236390.23601452449298413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143400.14317222730358273No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155800.051.475571
GTATCAA288250.042.0033071
CAACGCA349800.033.83625
ATCAACG353050.033.564813
TATCAAC370400.032.280492
TCAACGC369750.032.0107154
AACGCAG375550.031.7915926
ACGCAGA426400.027.692467
GTACATG425000.027.2264121
GTACTAG36600.026.9887311
CGCAGAG436500.026.900058
TACATGG436100.026.0151752
ACATGGG441200.024.8778423
GTGGTAT77600.024.7916741
GCAGAGT477900.024.0013969
CATGGGG304550.022.8338174
TGGTATC79550.022.4687522
GAGTACT255300.021.28600112-13
GCATCGT39750.021.0403968
TAGTACT57550.020.8194964
Produced by FastQC (version 0.11.5)