FastQCFastQC Report
Mon 6 Jun 2016
ERR1211353_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211353_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10015909
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270610.2701801703669632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221050.22069889013568317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136290.13607352063602016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149750.052.020581
GTATCAA274500.041.3036541
CAACGCA345200.032.0991365
ATCAACG348100.031.85143
TCAACGC363400.030.5880054
TATCAAC367900.030.5550122
AACGCAG370100.030.040796
GTACATG413700.026.4984951
ACGCAGA428250.025.8522827
TACATGG420700.025.7053722
CGCAGAG440200.025.1504768
GTGGTAT74350.024.5528851
ACATGGG427550.024.3742073
GTACTAG40500.024.1013971
GCAGAGT488050.022.4142729
CATGGGG285550.021.9037764
CTAGTAC53450.021.7707213
TGGTATC81250.021.3650462
CTGTGCG53200.021.0739869
TAGTACT54800.021.063264
Produced by FastQC (version 0.11.5)