##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211353_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10015909 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09110705778178 33.0 31.0 34.0 30.0 34.0 2 32.222855958455696 33.0 31.0 34.0 30.0 34.0 3 32.29153739316122 34.0 31.0 34.0 30.0 34.0 4 35.933810201350674 37.0 35.0 37.0 35.0 37.0 5 35.83716545348006 37.0 35.0 37.0 35.0 37.0 6 35.85125773407086 37.0 35.0 37.0 35.0 37.0 7 35.76514213537683 37.0 35.0 37.0 33.0 37.0 8 35.7451807918782 37.0 35.0 37.0 33.0 37.0 9 37.35839502934781 39.0 37.0 39.0 34.0 39.0 10-11 37.30576960114155 39.0 37.0 39.0 34.0 39.0 12-13 37.23636960958811 39.0 37.0 39.0 33.0 39.0 14-15 38.46705531170461 40.0 38.0 41.0 34.0 41.0 16-17 38.45362093445537 40.0 38.0 41.0 34.0 41.0 18-19 38.456847900674816 40.0 38.0 41.0 34.0 41.0 20-21 38.42186700178686 40.0 38.0 41.0 34.0 41.0 22-23 38.314851852188355 40.0 38.0 41.0 34.0 41.0 24-25 38.226349800103016 40.0 38.0 41.0 33.0 41.0 26-27 38.12940722604409 40.0 38.0 41.0 33.0 41.0 28-29 38.03007764946746 40.0 37.0 41.0 33.0 41.0 30-31 38.041683186219046 40.0 37.0 41.0 33.0 41.0 32-33 37.98486363045032 40.0 37.0 41.0 33.0 41.0 34-35 37.86052893451807 40.0 37.0 41.0 33.0 41.0 36-37 37.72003105259842 40.0 37.0 41.0 33.0 41.0 38-39 37.606463776777524 40.0 37.0 41.0 32.5 41.0 40-41 37.49765562965878 40.0 36.5 41.0 32.0 41.0 42-43 37.39127377255524 40.0 36.0 41.0 32.0 41.0 44-45 37.23251069872939 39.5 36.0 41.0 31.0 41.0 46-47 36.99349355110954 39.0 36.0 41.0 31.0 41.0 48-49 35.44743058268601 39.0 35.0 40.5 24.0 41.0 50-51 36.17611931178688 39.0 35.0 40.0 29.0 41.0 52-53 36.27014093279003 39.0 35.0 40.0 29.5 41.0 54-55 36.101159864771134 38.0 35.0 40.0 29.0 41.0 56-57 35.8849904686634 38.0 35.0 40.0 28.5 41.0 58-59 35.642955122695305 38.0 34.0 40.0 28.0 41.0 60-61 35.386807527903855 37.5 34.0 40.0 28.0 41.0 62-63 35.063330497511515 37.0 34.0 40.0 27.5 41.0 64-65 34.7277034965074 36.5 34.0 39.0 27.0 41.0 66-67 34.38959454403989 36.0 33.0 39.0 26.0 40.5 68-69 34.03017060158993 35.5 33.0 39.0 26.0 40.0 70-71 33.675083809167994 35.0 32.5 38.0 26.0 40.0 72-73 33.30028283004568 35.0 32.0 37.0 26.0 39.5 74-75 32.934580875285505 35.0 32.0 37.0 25.0 39.0 76-77 32.003026884529405 34.0 30.5 36.0 24.5 38.0 78-79 32.44305958650383 34.5 31.5 36.0 25.0 38.0 80-81 32.31714270766638 35.0 32.0 35.5 25.0 37.0 82-83 32.046803041042004 34.0 31.0 35.0 25.0 37.0 84-85 31.76885522821743 34.0 31.0 35.0 24.0 36.5 86-87 31.515123190516206 34.0 31.0 35.0 24.0 36.0 88-89 31.292300229564788 34.0 31.0 35.0 23.5 36.0 90-91 31.074102660078083 34.0 30.5 35.0 23.0 35.0 92-93 30.885236577129444 34.0 30.0 35.0 22.0 35.0 94-95 30.691005728985758 34.0 30.0 35.0 20.0 35.0 96-97 30.50381752669678 34.0 29.5 35.0 20.0 35.0 98-99 30.28801809201741 34.0 29.0 35.0 19.5 35.0 100 27.823880788054286 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2736309350413926 1101 2 -0.2784934419849705 1101 3 -0.2973088201169638 1101 4 -0.17785275959244018 1101 5 -0.12749763230966238 1101 6 -0.19458804196109014 1101 7 -0.19812705109559658 1101 8 -0.19116738430808766 1101 9 -0.33657087889213955 1101 10-11 -0.28235092765479664 1101 12-13 -0.3914561329677184 1101 14-15 -0.5009401264625737 1101 16-17 -0.5160108409776498 1101 18-19 -0.5018710343613009 1101 20-21 -0.5259053684287949 1101 22-23 -0.6519665153118837 1101 24-25 -0.5880569233553103 1101 26-27 -0.6361014695373299 1101 28-29 -0.7057518992822551 1101 30-31 -0.6208903051484427 1101 32-33 -0.5933131665702192 1101 34-35 -0.6540473365891799 1101 36-37 -0.648828561158382 1101 38-39 -0.5578243700557408 1101 40-41 -0.6150384239805291 1101 42-43 -0.6863225851494335 1101 44-45 -0.733236438650998 1101 46-47 -0.6082027911251515 1101 48-49 0.5787612942544911 1101 50-51 -0.3146921053466798 1101 52-53 -0.6691212682264123 1101 54-55 -0.6537209150543859 1101 56-57 -0.7233862096433441 1101 58-59 -0.879026557874802 1101 60-61 -0.8204354179265678 1101 62-63 -0.89883216547328 1101 64-65 -0.8939608227729039 1101 66-67 -0.8355941395448809 1101 68-69 -0.8951786916961453 1101 70-71 -0.7740931149583403 1101 72-73 -0.8990697570623212 1101 74-75 -0.9913424107150419 1101 76-77 -0.943353151635872 1101 78-79 -0.7750498761363716 1101 80-81 -0.6436178648694693 1101 82-83 -0.7390287198430165 1101 84-85 -0.7496466685368439 1101 86-87 -0.7717948274865556 1101 88-89 -0.7388837926370897 1101 90-91 -0.8039878319500637 1101 92-93 -0.8927028236562755 1101 94-95 -0.8648896491344225 1101 96-97 -0.9695901525573554 1101 98-99 -1.1322330390173008 1101 100 -0.9785666643487296 1102 1 -0.23445305686749052 1102 2 -0.2582279875142852 1102 3 -0.28471737019201626 1102 4 -0.17885530527273374 1102 5 -0.17308771420032798 1102 6 -0.24700600567875597 1102 7 -0.17124314782896022 1102 8 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-0.7972051497195807 1102 72-73 -0.8099223688629849 1102 74-75 -0.8950432791474974 1102 76-77 -0.8326495973243375 1102 78-79 -0.70293922268268 1102 80-81 -0.6526430632587363 1102 82-83 -0.6837642528670891 1102 84-85 -0.5833226928693307 1102 86-87 -0.648738267828211 1102 88-89 -0.7538260548586919 1102 90-91 -0.7040516206151644 1102 92-93 -0.890073539616342 1102 94-95 -0.8519138969451809 1102 96-97 -0.9372914774950374 1102 98-99 -1.0407946985394183 1102 100 -0.8241966489677495 1103 1 -0.33435983509206935 1103 2 -0.3304442494463551 1103 3 -0.3397099756870823 1103 4 -0.20936130677626608 1103 5 -0.20641288016443582 1103 6 -0.25434449887720234 1103 7 -0.25141573852110355 1103 8 -0.3089558434059043 1103 9 -0.4217198742790913 1103 10-11 -0.4132462127796188 1103 12-13 -0.5023944802858296 1103 14-15 -0.6100212157745446 1103 16-17 -0.5910851305027762 1103 18-19 -0.590041654338691 1103 20-21 -0.5893911733902684 1103 22-23 -0.6321551760813193 1103 24-25 -0.7193148134617218 1103 26-27 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-0.9332548228451785 1103 92-93 -0.8983568374753759 1103 94-95 -0.929902780407307 1103 96-97 -1.085468545495015 1103 98-99 -1.1524442192778324 1103 100 -1.1503302557112818 1104 1 -0.24928141311970364 1104 2 -0.24764451667550702 1104 3 -0.31831482347193685 1104 4 -0.15256260257368126 1104 5 -0.16580801082493224 1104 6 -0.2353152958456448 1104 7 -0.2505687620867434 1104 8 -0.26830922102475085 1104 9 -0.3904837432699608 1104 10-11 -0.3213235736095754 1104 12-13 -0.35165252825121485 1104 14-15 -0.4939465066502251 1104 16-17 -0.5531861622073606 1104 18-19 -0.5783885596306106 1104 20-21 -0.5166482564825685 1104 22-23 -0.48906319139383925 1104 24-25 -0.5828719522207138 1104 26-27 -0.5866452327014215 1104 28-29 -0.5832894043913797 1104 30-31 -0.512756458162194 1104 32-33 -0.5460619286131845 1104 34-35 -0.5383410438746097 1104 36-37 -0.5224567117221355 1104 38-39 -0.4867856873148284 1104 40-41 -0.5018410093041439 1104 42-43 -0.5585285361889234 1104 44-45 -0.5614320373448152 1104 46-47 -0.5902800747899306 1104 48-49 0.6502797805775842 1104 50-51 -0.2037384518541785 1104 52-53 -0.5800234718438944 1104 54-55 -0.5513314246784944 1104 56-57 -0.5515041117927737 1104 58-59 -0.6965834512509517 1104 60-61 -0.5962789308609544 1104 62-63 -0.6368534689705001 1104 64-65 -0.6829568399430954 1104 66-67 -0.7123125197192621 1104 68-69 -0.6484539286068696 1104 70-71 -0.7196277365503789 1104 72-73 -0.7571471443620084 1104 74-75 -0.67057661744726 1104 76-77 -0.5915325564844913 1104 78-79 -0.435179228877022 1104 80-81 -0.47636637434678875 1104 82-83 -0.6352802247133233 1104 84-85 -0.5231761109916597 1104 86-87 -0.5558306450895749 1104 88-89 -0.5651614991181013 1104 90-91 -0.7244173156809808 1104 92-93 -0.6866481729563425 1104 94-95 -0.7273934746739315 1104 96-97 -0.8008973600101505 1104 98-99 -0.7924909868016634 1104 100 -0.6771145897472408 1105 1 -0.2745825987776662 1105 2 -0.24360789071088718 1105 3 -0.30381436058809896 1105 4 -0.2103658514154887 1105 5 -0.25413900486524454 1105 6 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1105 68-69 -0.7830913654623828 1105 70-71 -0.8205656626348699 1105 72-73 -0.644751503548811 1105 74-75 -0.710357176049456 1105 76-77 -0.7143049028025032 1105 78-79 -0.6045668646175812 1105 80-81 -0.6046400256480311 1105 82-83 -0.6420720553696384 1105 84-85 -0.6388144452644333 1105 86-87 -0.5891744215924533 1105 88-89 -0.6370099504999693 1105 90-91 -0.6792795609205626 1105 92-93 -0.665124667988163 1105 94-95 -0.6848508312194106 1105 96-97 -0.6695293017263175 1105 98-99 -0.72534912999037 1105 100 -0.7800138917393866 1106 1 -0.3626966056490417 1106 2 -0.3010238600413082 1106 3 -0.37777563737776276 1106 4 -0.20789123832769718 1106 5 -0.29427232663245206 1106 6 -0.3154668199407098 1106 7 -0.32005330219376305 1106 8 -0.4200132438963493 1106 9 -0.498916645201426 1106 10-11 -0.4999478631346008 1106 12-13 -0.5174357433454944 1106 14-15 -0.637399213339684 1106 16-17 -0.6516644776259071 1106 18-19 -0.6446169614149824 1106 20-21 -0.6931011247331327 1106 22-23 -0.7352216351017873 1106 24-25 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0.4098083184350614 2311 10-11 0.37463310679587636 2311 12-13 0.47356073462745485 2311 14-15 0.4209512428360114 2311 16-17 0.41771123834707424 2311 18-19 0.5974803962343316 2311 20-21 0.6367815889464197 2311 22-23 0.694677020201496 2311 24-25 0.6117980180526104 2311 26-27 0.6563756492285435 2311 28-29 0.7537949619214785 2311 30-31 0.6216689899549266 2311 32-33 0.6889746803094425 2311 34-35 0.803356596112593 2311 36-37 0.8173275385371568 2311 38-39 0.8203816804284472 2311 40-41 0.7803849153651399 2311 42-43 0.8611515550345743 2311 44-45 0.8652453414640391 2311 46-47 0.9063667098323904 2311 48-49 -5.988699227607853 2311 50-51 -1.0230001140804816 2311 52-53 0.4574062116499036 2311 54-55 0.8593288275548616 2311 56-57 0.9214843734576235 2311 58-59 0.9314741359916354 2311 60-61 0.8103203142125608 2311 62-63 0.7396835659959322 2311 64-65 0.8122663607447436 2311 66-67 0.8927792821183118 2311 68-69 0.960285560063447 2311 70-71 0.9270520616475082 2311 72-73 0.43783170767162005 2311 74-75 0.7277554499640573 2311 76-77 0.8345545781492127 2311 78-79 0.7197634576279981 2311 80-81 0.766968208843565 2311 82-83 0.8120062521549976 2311 84-85 0.8268789758942852 2311 86-87 0.8724378434766429 2311 88-89 0.9428453291496162 2311 90-91 0.9966264530909719 2311 92-93 0.9933722038900079 2311 94-95 0.981162391043231 2311 96-97 1.0910893535920216 2311 98-99 1.0777420456663442 2311 100 1.3098309267682247 2312 1 0.09692431877186891 2312 2 0.2692055001873541 2312 3 0.2451718239805345 2312 4 0.034030493411229656 2312 5 0.21831408866967195 2312 6 0.13878865599450307 2312 7 0.3040126867854198 2312 8 0.35270363799057947 2312 9 0.2714195244196773 2312 10-11 0.25367404829410845 2312 12-13 0.3192816373952354 2312 14-15 0.359115740175163 2312 16-17 0.481795634546323 2312 18-19 0.3458042774197594 2312 20-21 0.3958651010064571 2312 22-23 0.6389084098123803 2312 24-25 0.7030379815508496 2312 26-27 0.6624628267596364 2312 28-29 0.7550612591901427 2312 30-31 0.781323255626198 2312 32-33 0.780511228423947 2312 34-35 0.4108969770056916 2312 36-37 0.7463863969253666 2312 38-39 0.8286088728361776 2312 40-41 0.6002437472838054 2312 42-43 0.6369653727960767 2312 44-45 0.8127913752295726 2312 46-47 0.8376409378220089 2312 48-49 -4.684731171019195 2312 50-51 -0.745751321987882 2312 52-53 0.5713860289288348 2312 54-55 0.8663169530892034 2312 56-57 0.968852701484046 2312 58-59 0.9919421413891811 2312 60-61 1.0431408259925945 2312 62-63 0.7803578949943599 2312 64-65 0.9646213314799681 2312 66-67 0.9229640992084001 2312 68-69 0.8807618184674553 2312 70-71 0.9002844036004163 2312 72-73 1.022385538093566 2312 74-75 0.7035725491072 2312 76-77 0.8210161944899177 2312 78-79 0.5697913630280595 2312 80-81 0.7750598657532279 2312 82-83 0.7864941810405561 2312 84-85 0.7990684783759683 2312 86-87 0.8841988927196844 2312 88-89 0.8396875319157466 2312 90-91 0.6596470300460417 2312 92-93 0.9056277702213613 2312 94-95 1.0466617416340043 2312 96-97 1.0826322537746513 2312 98-99 1.0905271200854258 2312 100 0.7340350376019806 2313 1 0.07837045101064177 2313 2 0.23757365602003944 2313 3 0.2864065317570379 2313 4 0.06351312224497718 2313 5 0.17567718770202845 2313 6 0.24113311243731061 2313 7 0.14113554169818343 2313 8 0.2546406896781761 2313 9 0.43610721383929985 2313 10-11 0.18589014380187763 2313 12-13 0.06824988293863044 2313 14-15 0.10424890063936942 2313 16-17 0.11053797607306848 2313 18-19 0.4377865853481282 2313 20-21 0.4298821797652437 2313 22-23 0.4986502706418605 2313 24-25 0.3061154238488655 2313 26-27 0.42002038381615847 2313 28-29 0.44725677133340724 2313 30-31 0.6055137530172274 2313 32-33 0.47636779239491034 2313 34-35 0.42287093638133655 2313 36-37 -0.3619965606582767 2313 38-39 0.18397606043848214 2313 40-41 0.39850724740458077 2313 42-43 0.49102665780844745 2313 44-45 0.5159538494770075 2313 46-47 0.40070086453496856 2313 48-49 -5.321229039736586 2313 50-51 -1.3201522915307535 2313 52-53 0.10547172067489186 2313 54-55 0.2519887695947105 2313 56-57 0.18594401090467017 2313 58-59 0.4244106584071261 2313 60-61 0.6979726568406335 2313 62-63 0.7621325242047163 2313 64-65 0.8300238378139895 2313 66-67 0.8978245087878705 2313 68-69 0.8972476998309915 2313 70-71 0.8930283456892383 2313 72-73 0.5898829633311209 2313 74-75 0.752549100972935 2313 76-77 0.8523124096111481 2313 78-79 0.7488458456060059 2313 80-81 -0.2840903929139742 2313 82-83 0.15638932845605424 2313 84-85 0.5757477082285867 2313 86-87 0.7448206381125537 2313 88-89 0.7460749867015046 2313 90-91 0.7497242765651997 2313 92-93 0.8321793427761897 2313 94-95 0.7521084469300305 2313 96-97 0.7919317604052338 2313 98-99 0.9591107595105868 2313 100 1.1029870801962183 2314 1 0.0637139485828726 2314 2 0.1908581478133229 2314 3 0.1875572177363054 2314 4 0.07216810157019893 2314 5 0.2013981764849575 2314 6 0.24714446277179292 2314 7 0.06686589595154402 2314 8 0.23303532786939485 2314 9 0.38007403935389306 2314 10-11 0.2992480781698177 2314 12-13 0.1866187953902454 2314 14-15 0.2168905718797447 2314 16-17 0.42028727243438624 2314 18-19 0.5183087852130654 2314 20-21 0.5993596468992308 2314 22-23 0.32911341091488566 2314 24-25 0.4129615886561666 2314 26-27 0.4784662399418096 2314 28-29 0.5830246203246006 2314 30-31 0.5459049313123927 2314 32-33 0.47595327264788523 2314 34-35 0.4117122107199407 2314 36-37 0.4813805169798755 2314 38-39 0.503015576389295 2314 40-41 0.25281399035977614 2314 42-43 0.3112623506463521 2314 44-45 0.5006557649643568 2314 46-47 0.5546624052483935 2314 48-49 -6.360736606320376 2314 50-51 -1.5801074476810228 2314 52-53 -0.8394626357837893 2314 54-55 -0.3767500037548075 2314 56-57 -0.14412664689294274 2314 58-59 0.36429728086270075 2314 60-61 0.677942419750373 2314 62-63 0.5481445858295118 2314 64-65 0.6493749761774481 2314 66-67 0.7452740194597993 2314 68-69 0.8199085463096836 2314 70-71 0.5758848416997111 2314 72-73 0.7213372495049697 2314 74-75 0.764606041553165 2314 76-77 0.6844606759601497 2314 78-79 0.4996955311671414 2314 80-81 0.536179702635728 2314 82-83 0.5502265262694088 2314 84-85 0.5795839990949965 2314 86-87 0.6558222879035149 2314 88-89 0.5296505016085042 2314 90-91 0.7538825202778305 2314 92-93 0.7756587583068999 2314 94-95 0.8808229260006009 2314 96-97 0.8610852663264659 2314 98-99 0.8630196786530107 2314 100 -0.5421558370602071 2315 1 0.10025151681062283 2315 2 0.19106994153781187 2315 3 0.27717202751904324 2315 4 0.08584564361096625 2315 5 0.1957907742227576 2315 6 0.253753239789539 2315 7 0.31311538788795445 2315 8 0.23547488405608874 2315 9 0.38807415097640074 2315 10-11 0.1861992218595958 2315 12-13 0.39660756726379987 2315 14-15 0.1989454057305693 2315 16-17 0.35115799076026377 2315 18-19 0.4033278274283134 2315 20-21 0.3146062781275063 2315 22-23 0.40130204857440077 2315 24-25 0.4331516868568315 2315 26-27 0.5246889603767784 2315 28-29 0.5959570623446169 2315 30-31 0.578197275491938 2315 32-33 0.6271906590151772 2315 34-35 0.6541777760651115 2315 36-37 0.5683241150001166 2315 38-39 0.6760203817175423 2315 40-41 0.7034522391224058 2315 42-43 0.7441358404909337 2315 44-45 0.7597193604334862 2315 46-47 0.6993516895276244 2315 48-49 -4.789247626673159 2315 50-51 -0.8164576535451715 2315 52-53 0.3377477725921594 2315 54-55 0.3106192335555278 2315 56-57 0.6352403271860325 2315 58-59 0.7090022118058812 2315 60-61 0.5783574191035683 2315 62-63 0.32171164623986215 2315 64-65 0.5309288534242995 2315 66-67 -0.18751040566208133 2315 68-69 0.5113125600747495 2315 70-71 0.5006073688860155 2315 72-73 0.6514668035010232 2315 74-75 0.8412332766313213 2315 76-77 0.8162587604457947 2315 78-79 0.7216065892761492 2315 80-81 0.6036675811110968 2315 82-83 0.647692562650402 2315 84-85 0.6528997628877526 2315 86-87 0.6740159270963026 2315 88-89 0.6491617954569584 2315 90-91 0.7235306991445007 2315 92-93 0.7758918113647546 2315 94-95 0.41248780073581415 2315 96-97 0.5609257362302138 2315 98-99 0.4914290452071839 2315 100 0.5522181583941119 2316 1 0.22008507857525217 2316 2 0.24549508612489745 2316 3 0.1918109881932395 2316 4 -0.0435604961488778 2316 5 0.12465491621250635 2316 6 0.1413403453104678 2316 7 0.2340266717254238 2316 8 0.28455098312706895 2316 9 0.42664757443689183 2316 10-11 0.40089724150043793 2316 12-13 0.3245833670059284 2316 14-15 0.43029072664078427 2316 16-17 0.42298654600588037 2316 18-19 0.1298483859888151 2316 20-21 0.4621313756854164 2316 22-23 0.5704931008424836 2316 24-25 0.6244132925832417 2316 26-27 0.6126365478830635 2316 28-29 0.6561315491962105 2316 30-31 0.5956602441942422 2316 32-33 0.5444872618972241 2316 34-35 0.5101474642727339 2316 36-37 0.4224880397535671 2316 38-39 0.5443544763881576 2316 40-41 0.163542960054464 2316 42-43 0.11176333076164013 2316 44-45 0.38430695560703754 2316 46-47 0.4326697426697237 2316 48-49 -5.163368772913177 2316 50-51 -1.1149364671862756 2316 52-53 0.202493126274355 2316 54-55 0.47937205394355686 2316 56-57 0.39239988228202805 2316 58-59 0.3005872398410716 2316 60-61 0.45272589457331947 2316 62-63 0.35267235933934415 2316 64-65 0.3608977331420178 2316 66-67 0.4492942590696458 2316 68-69 0.1873198091438084 2316 70-71 -0.2302852371213504 2316 72-73 -0.01863218524975707 2316 74-75 0.18154587250418786 2316 76-77 0.19722040620979087 2316 78-79 0.26113886938961883 2316 80-81 0.18297784085132207 2316 82-83 0.10284674827860485 2316 84-85 0.3041071539325877 2316 86-87 0.4101089921402199 2316 88-89 0.25132337456291154 2316 90-91 0.2678103169035033 2316 92-93 0.3432674769477231 2316 94-95 0.5301771901210337 2316 96-97 0.5857442851824821 2316 98-99 0.5642731810237329 2316 100 -0.2479375280787366 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 7.0 10 16.0 11 18.0 12 34.0 13 218.0 14 787.0 15 2268.0 16 5382.0 17 10976.0 18 18919.0 19 28554.0 20 41319.0 21 58119.0 22 73421.0 23 77570.0 24 81034.0 25 90904.0 26 108995.0 27 133621.0 28 164769.0 29 202283.0 30 249955.0 31 309089.0 32 390692.0 33 507620.0 34 704046.0 35 988057.0 36 1528778.0 37 2234071.0 38 1776750.0 39 227621.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.21797371638997 19.800933432884595 14.01986950475645 23.961223345968993 2 15.456023949568042 20.16939782519727 40.28124607803758 24.093332147197106 3 18.913557443664573 25.313831008696177 29.56418294911091 26.20842859852834 4 12.77171809934069 16.486610614460815 37.8964554451273 32.845215841071195 5 13.511108679412715 37.809905927218246 34.498338735098386 14.180646658270652 6 31.696915564868743 36.89840415770841 17.399221757561126 14.005458519861715 7 28.25307066579898 32.06927061815476 21.639939512211875 18.03771920383438 8 26.404944975782037 35.63695628145936 19.569049929514247 18.38904881324435 9 26.598283443162668 15.90446145815069 20.23390673047006 37.26334836821658 10-11 25.48625378139699 26.21197149426472 27.445401564666415 20.856373159671875 12-13 25.71519783544165 23.608861909563792 28.010847752074202 22.665092502920356 14-15 23.084511398005024 25.122436606404968 25.513425032554498 26.27962696303551 16-17 22.423334592833687 28.131951307506636 26.42332900171043 23.02138509794924 18-19 22.16486124697138 27.345180653874852 28.5648205117709 21.92513758738287 20-21 23.0979722946598 26.43013815129799 28.486298239909075 21.985591314133135 22-23 23.03750108140724 26.23855636514179 28.183047637979453 22.54089491547152 24-25 22.696614899172122 26.393419945905038 28.52394623731224 22.386018917610595 26-27 22.595454837490163 26.630373711243067 28.211983714097027 22.56218773716974 28-29 22.323664054763015 26.73201900475159 28.421762351545972 22.52255458893943 30-31 22.865511313944353 26.512441028042904 28.326203590043864 22.295844067968883 32-33 22.765196109291978 26.74691770770204 28.027468226501885 22.4604179565041 34-35 22.824349514489427 26.71015169914971 28.330956850128374 22.134541936232495 36-37 22.78849522751556 26.53500148969281 28.137543459359797 22.53895982343183 38-39 22.768181179749682 26.60793717439252 28.286158055542614 22.337723590315182 40-41 22.723228297536256 26.238683930446122 28.570674023560677 22.46741374845695 42-43 22.559828655750202 26.33460813769365 28.245531653126342 22.860031553429806 44-45 22.5231469011762 26.729626550729925 28.134678761655703 22.612547786438174 46-47 22.57593066144317 26.590915365747435 27.840947131407383 22.99220684140201 48-49 22.881530295786458 26.675780863541604 27.735176141278036 22.7075126993939 50-51 22.73751013323443 26.704026072095704 27.61171822308554 22.946745571584323 52-53 22.54687380324621 26.90255080127768 27.55410413089824 22.996471264577863 54-55 22.517589909111106 26.808054937730745 27.56336885513943 23.11098629801872 56-57 22.74429827167101 26.720473402085737 27.341242006497147 23.193986319746106 58-59 22.449763611969566 26.7730749794735 27.702506445017377 23.074654963539558 60-61 22.61028950159517 27.044040416748103 27.419357334864973 22.926312746791755 62-63 22.617576576001323 26.814856610692715 27.38735844029322 23.180208373012746 64-65 22.289349519578913 26.679678814872275 27.498369653490762 23.53260201205805 66-67 22.452535589818652 26.648795522929447 27.344887274272907 23.553781612978998 68-69 22.25605784108134 26.74270650939371 27.46227948250029 23.53895616702466 70-71 22.36878760676926 26.427823240638233 27.520082035381055 23.683307117211456 72-73 22.477851016444273 26.694033424614116 27.22962456541016 23.598490993531453 74-75 22.509299224983454 26.79997074373081 27.156198272631983 23.534531758653753 76-77 22.366491141764964 26.671215765117534 27.152935234461722 23.80935785865578 78-79 22.28820712514137 26.569024298394655 27.200077323353856 23.94269125311012 80-81 22.235455406471036 26.941033059674837 27.090823697243295 23.73268783661084 82-83 22.58320807258077 26.554372519769696 27.390165384135695 23.472254023513837 84-85 22.605999334198465 26.604004226496553 26.95449126176681 23.83550517753817 86-87 22.128844715120323 26.853954515261318 27.25266488071719 23.764535888901168 88-89 22.36101657758737 26.423511739940064 27.215677422930973 23.999794259541588 90-91 22.434786663958413 26.40773366743141 27.205445750705078 23.952033917905105 92-93 22.47687328246836 26.49282366907321 27.067966634758108 23.96233641370032 94-95 22.078869659260974 26.284223387234988 27.307603193551273 24.329303759952765 96-97 22.192913309174198 26.208689800395085 27.267474977622264 24.33092191280845 98-99 21.98105277926106 26.302176441468394 27.21244588104277 24.50432489822778 100 22.07632497540562 26.151581281304775 27.059221269519444 24.712872473770155 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1361.0 1 985.5 2 832.5 3 1060.5 4 873.5 5 865.0 6 1482.0 7 2769.5 8 4391.0 9 5442.0 10 5234.5 11 4742.0 12 5130.5 13 5885.0 14 6631.5 15 7613.5 16 8614.5 17 9546.5 18 10541.0 19 11609.0 20 12777.0 21 14098.0 22 15965.5 23 18653.0 24 21836.5 25 25799.5 26 31849.5 27 39469.5 28 48265.5 29 61387.0 30 79722.0 31 99453.0 32 125244.0 33 157432.0 34 188453.0 35 218087.5 36 252328.5 37 287777.0 38 306473.0 39 311132.0 40 307451.0 41 302376.5 42 306157.0 43 319331.5 44 336898.0 45 362353.5 46 385203.0 47 398593.0 48 412119.5 49 419037.5 50 414337.0 51 407689.5 52 398974.5 53 385160.5 54 372047.0 55 344411.5 56 308272.0 57 268918.5 58 232055.5 59 203744.0 60 168828.0 61 133438.0 62 105929.0 63 82517.5 64 61314.0 65 43760.5 66 31446.0 67 23740.0 68 18121.5 69 13279.5 70 9891.0 71 7541.0 72 5926.5 73 4582.5 74 3368.0 75 2331.5 76 1502.5 77 856.5 78 479.0 79 294.0 80 183.5 81 108.0 82 66.5 83 37.5 84 26.5 85 21.5 86 15.5 87 11.0 88 7.5 89 6.0 90 4.5 91 3.5 92 3.0 93 2.5 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029722714134084085 2 0.001956886788807686 3 8.985704642484272E-5 4 0.022643975699060365 5 4.6925346466306756E-4 6 6.988881388598877E-5 7 2.9952348808280904E-5 8 0.004093487670465057 9 0.009754481595230149 10-11 9.98411626942697E-4 12-13 8.985704642484272E-5 14-15 1.1481733709841013E-4 16-17 3.244837787563765E-4 18-19 1.4976174404140452E-5 20-21 5.990469761656181E-5 22-23 2.4461084860096075E-4 24-25 3.494440694299439E-5 26-27 7.488087202070227E-5 28-29 9.78443394403843E-4 30-31 0.002371227613988905 32-33 0.009180394909738097 34-35 0.01809121868020167 36-37 0.02096165210766192 38-39 0.02027274808507146 40-41 0.004497844379376849 42-43 0.0051518039950243155 44-45 0.009060585514504975 46-47 0.011616519279478278 48-49 1.2016682659556912 50-51 0.01828091688932078 52-53 0.018370773935745623 54-55 0.009325164595644789 56-57 0.007428182504453666 58-59 0.00806716594569699 60-61 0.00505695489046476 62-63 0.007433174562588378 64-65 0.019304288806937044 66-67 0.0292834130182293 68-69 0.02951803975056083 70-71 0.026642614264965864 72-73 0.02296845947781674 74-75 0.00959972779305403 76-77 0.014681642974192357 78-79 0.008221919747873108 80-81 0.0026258225788592924 82-83 0.014636714450979936 84-85 0.024106648732531415 86-87 0.023707284081754338 88-89 0.03288767899149243 90-91 0.020796914189216375 92-93 0.013533469603208255 94-95 0.006624461144764794 96-97 0.003734059484765686 98-99 0.015090991741238863 100 0.03453505817594788 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0015909E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.051969834143634 #Duplication Level Percentage of deduplicated Percentage of total 1 72.3081342405734 39.083970907346824 2 15.192448350681328 16.423635199156248 3 5.435161862039104 8.813436150318767 4 2.597955270178242 5.616983995765152 5 1.3562577296577325 3.6654200945391944 6 0.7922234581078013 2.5692743075726296 7 0.5372189829481755 2.0326420982450872 8 0.35010780941983005 1.5139213402767009 9 0.23557834508257552 1.1460126241782769 >10 1.1007676138123745 10.197152957401952 >50 0.054554917722420264 2.0375966696333765 >100 0.035154472646957494 3.768203661395006 >500 0.003004823044068719 1.0743368082226212 >1k 0.0013198006289403342 1.0213906739550653 >5k 2.8080864263542937E-5 0.08290365765425968 >10k+ 8.424259279062881E-5 0.9531188543389302 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27061 0.2701801703669632 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22105 0.22069889013568317 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13629 0.13607352063602016 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.98411626942697E-6 2 0.0 0.0 0.0 0.0 9.98411626942697E-6 3 0.0 1.996823253885394E-5 0.0 1.996823253885394E-5 9.98411626942697E-6 4 0.0 1.996823253885394E-5 0.0 1.996823253885394E-5 9.98411626942697E-6 5 0.0 1.996823253885394E-5 0.0 2.9952348808280904E-5 9.98411626942697E-6 6 0.0 1.996823253885394E-5 0.0 2.9952348808280904E-5 9.98411626942697E-6 7 0.0 1.996823253885394E-5 0.0 2.9952348808280904E-5 9.98411626942697E-6 8 0.0 1.996823253885394E-5 0.0 2.9952348808280904E-5 2.9952348808280904E-5 9 0.0 1.996823253885394E-5 0.0 5.990469761656181E-5 2.9952348808280904E-5 10-11 0.0 1.996823253885394E-5 0.0 6.988881388598879E-5 2.9952348808280904E-5 12-13 0.0 1.996823253885394E-5 0.0 8.486498829012924E-5 2.9952348808280904E-5 14-15 0.0 2.496029067356742E-5 0.0 1.297935115025506E-4 4.492852321242136E-5 16-17 0.0 2.9952348808280904E-5 0.0 1.9968232538853937E-4 4.992058134713484E-5 18-19 0.0 2.9952348808280904E-5 0.0 2.496029067356742E-4 6.988881388598877E-5 20-21 0.0 3.993646507770788E-5 0.0 3.3446789502580344E-4 6.988881388598877E-5 22-23 0.0 3.993646507770788E-5 0.0 5.341502204143428E-4 9.984116269426969E-5 24-25 0.0 3.993646507770788E-5 0.0 9.584751618649889E-4 1.2979351150255058E-4 26-27 0.0 3.993646507770788E-5 0.0 0.002081688242175523 1.3977762777197755E-4 28-29 0.0 3.993646507770788E-5 0.0 0.005331518087874002 1.4476968590669103E-4 30-31 0.0 3.993646507770788E-5 0.0 0.018465623040305176 1.5475380217611803E-4 32-33 0.0 3.993646507770788E-5 0.0 0.048188337174389265 2.196505579273933E-4 34-35 0.0 3.993646507770788E-5 0.0 0.09470932693178422 2.3961879046624724E-4 36-37 0.0 3.993646507770788E-5 0.0 0.16938552456896322 2.3961879046624724E-4 38-39 0.0 3.993646507770788E-5 0.0 0.27537191082706525 2.3961879046624724E-4 40-41 0.0 3.993646507770788E-5 0.0 0.390718406087755 2.3961879046624724E-4 42-43 0.0 3.993646507770788E-5 0.0 0.5025455003634718 2.3961879046624724E-4 44-45 4.992058134713485E-6 3.993646507770788E-5 0.0 0.6368767927104769 2.446108486009607E-4 46-47 1.996823253885394E-5 4.492852321242136E-5 0.0 0.7963830342308422 2.496029067356742E-4 48-49 1.996823253885394E-5 5.4912639481848326E-5 0.0 0.9674808347400121 2.496029067356742E-4 50-51 1.996823253885394E-5 5.990469761656181E-5 0.0 1.1535348414207838 2.496029067356742E-4 52-53 1.996823253885394E-5 6.988881388598877E-5 0.0 1.3344769805716088 2.845473136786686E-4 54-55 1.996823253885394E-5 6.988881388598877E-5 0.0 1.5354123125519612 3.19491720621663E-4 56-57 1.996823253885394E-5 6.988881388598877E-5 0.0 1.7641584004008024 3.244837787563765E-4 58-59 1.996823253885394E-5 6.988881388598877E-5 0.0 2.0214640528383394 3.3945995316051695E-4 60-61 1.996823253885394E-5 6.988881388598877E-5 0.0 2.287141386767791 3.594281856993709E-4 62-63 1.996823253885394E-5 6.988881388598877E-5 0.0 2.5563980263798323 3.893805345076518E-4 64-65 1.996823253885394E-5 6.988881388598877E-5 0.0 2.828520107361199 3.893805345076518E-4 66-67 2.496029067356742E-5 7.987293015541575E-5 0.0 3.1307542830111577 3.893805345076518E-4 68-69 2.9952348808280904E-5 7.987293015541575E-5 0.0 3.461807610272817 3.893805345076518E-4 70-71 3.4944406942994394E-5 8.985704642484272E-5 0.0 3.8205818363565403 3.9936465077707874E-4 72-73 3.993646507770788E-5 8.985704642484272E-5 0.0 4.178886808975601 5.191740460102023E-4 74-75 3.993646507770788E-5 8.985704642484272E-5 0.0 4.527172720918291 5.241661041449158E-4 76-77 3.993646507770788E-5 9.984116269426969E-5 0.0 4.903973268926465 5.391422785490563E-4 78-79 3.993646507770788E-5 9.984116269426969E-5 0.0 5.31682646078354 5.491263948184833E-4 80-81 3.993646507770788E-5 9.984116269426969E-5 0.0 5.754260546895944 5.641025692226237E-4 82-83 3.993646507770788E-5 9.984116269426969E-5 0.0 6.196981222573008 5.890628598961912E-4 84-85 3.993646507770788E-5 9.984116269426969E-5 0.0 6.628245124830906 5.890628598961912E-4 86-87 3.993646507770788E-5 9.984116269426969E-5 0.0 7.070321825008595 5.890628598961912E-4 88 3.993646507770788E-5 9.984116269426969E-5 0.0 7.423789493295117 6.090310924350451E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14975 0.0 52.02058 1 GTATCAA 27450 0.0 41.303654 1 CAACGCA 34520 0.0 32.099136 5 ATCAACG 34810 0.0 31.8514 3 TCAACGC 36340 0.0 30.588005 4 TATCAAC 36790 0.0 30.555012 2 AACGCAG 37010 0.0 30.04079 6 GTACATG 41370 0.0 26.498495 1 ACGCAGA 42825 0.0 25.852282 7 TACATGG 42070 0.0 25.705372 2 CGCAGAG 44020 0.0 25.150476 8 GTGGTAT 7435 0.0 24.552885 1 ACATGGG 42755 0.0 24.374207 3 GTACTAG 4050 0.0 24.101397 1 GCAGAGT 48805 0.0 22.414272 9 CATGGGG 28555 0.0 21.903776 4 CTAGTAC 5345 0.0 21.770721 3 TGGTATC 8125 0.0 21.365046 2 CTGTGCG 5320 0.0 21.073986 9 TAGTACT 5480 0.0 21.06326 4 >>END_MODULE