FastQCFastQC Report
Mon 6 Jun 2016
ERR1211352_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211352_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4403269
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194910.44264840508267833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147930.33595494620019806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103690.23548413689919923No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68900.15647465553433143No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77150.051.160571
TACCTGG49100.048.2895742
GTATCAA140850.042.9863931
ACCTGGG59650.038.8397673
GTACCTG64950.036.9402921
TAACGCA5800.036.4598434
ATAACGC5550.036.4095763
ATCAACG183250.032.6712383
TATCAAC183950.032.5561452
CAACGCA185150.032.4110075
TCAACGC188100.031.9780124
GTACATG176650.031.7447511
AACGCAG196350.030.9923366
TACATGG180800.030.3912662
ACATGGG188550.028.1638533
TATAACG7050.028.026222
ACGCAGA218300.027.5965467
CCTGGGG62100.026.7881014
CATGGGG133650.026.7223664
CGCAGAG226300.026.577638
Produced by FastQC (version 0.11.5)