##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211352_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4403269 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.439760096419274 31.0 31.0 34.0 28.0 34.0 2 31.431504638939842 31.0 31.0 34.0 28.0 34.0 3 31.484225242654947 33.0 31.0 34.0 28.0 34.0 4 35.09678899926396 37.0 35.0 37.0 32.0 37.0 5 35.146197291148916 37.0 35.0 37.0 32.0 37.0 6 35.20891115214628 37.0 35.0 37.0 32.0 37.0 7 35.150277214496775 37.0 35.0 37.0 32.0 37.0 8 35.147417293833286 37.0 35.0 37.0 32.0 37.0 9 36.70920990745739 39.0 37.0 39.0 32.0 39.0 10-11 36.630387900443964 39.0 37.0 39.0 32.0 39.0 12-13 36.54271871648086 39.0 37.0 39.0 32.0 39.0 14-15 37.67895375004343 40.0 37.0 41.0 32.0 41.0 16-17 37.675082421718955 40.0 37.0 41.0 32.0 41.0 18-19 37.67478514258384 40.0 37.0 41.0 32.0 41.0 20-21 37.6323245297982 40.0 37.0 41.0 32.0 41.0 22-23 37.500715150493875 40.0 37.0 41.0 31.5 41.0 24-25 37.387095019632 40.0 37.0 41.0 31.0 41.0 26-27 37.24318602838028 40.0 36.5 41.0 31.0 41.0 28-29 37.08782372823464 40.0 36.0 41.0 30.5 41.0 30-31 36.87762410154819 39.0 36.0 41.0 30.0 41.0 32-33 36.71573812546997 39.0 35.5 41.0 30.0 41.0 34-35 36.54822269545649 39.0 35.0 41.0 30.0 41.0 36-37 36.44130338164668 39.0 35.0 41.0 29.5 41.0 38-39 36.28256642962309 39.0 35.0 41.0 29.0 41.0 40-41 36.09708434801507 39.0 35.0 40.0 28.0 41.0 42-43 35.93814867999207 39.0 35.0 40.0 27.0 41.0 44-45 35.79819209319258 38.5 35.0 40.0 27.0 41.0 46-47 35.61332024003076 38.0 34.5 40.0 26.0 41.0 48-49 35.35716623263308 38.0 34.0 40.0 25.0 41.0 50-51 34.676149242755784 37.5 33.5 39.5 24.0 40.5 52-53 34.91243051469261 38.0 33.5 40.0 24.5 40.5 54-55 35.26721908200476 38.0 34.0 40.0 26.0 41.0 56-57 35.166801187935604 38.0 34.0 40.0 25.5 41.0 58-59 34.95215463783839 38.0 34.0 40.0 25.0 41.0 60-61 34.6786943972762 37.5 33.5 40.0 25.0 41.0 62-63 34.38474188154301 37.0 33.0 40.0 24.0 41.0 64-65 34.10373929005927 36.0 33.0 40.0 24.0 41.0 66-67 33.87350182330446 36.0 33.0 39.0 23.0 41.0 68-69 33.52544916061227 35.5 32.5 39.0 23.0 41.0 70-71 33.16886624460145 35.0 32.0 38.5 22.5 40.0 72-73 32.792403098697804 35.0 32.0 37.5 22.0 40.0 74-75 32.4191531337286 35.0 31.0 37.0 21.0 39.0 76-77 32.06056432164376 35.0 31.0 36.5 20.0 39.0 78-79 31.701304985000917 35.0 31.0 36.0 20.0 38.5 80-81 31.37458374675724 35.0 30.5 35.5 20.0 37.0 82-83 31.067582970742873 34.0 30.0 35.0 19.0 37.0 84-85 30.766594886662613 34.0 30.0 35.0 18.0 36.5 86-87 30.511073704559045 34.0 29.5 35.0 18.0 36.0 88-89 30.253156461710606 34.0 29.0 35.0 17.5 36.0 90-91 29.99645910799454 34.0 29.0 35.0 16.5 35.5 92-93 29.755569895911425 34.0 29.0 35.0 15.0 35.0 94-95 29.526594105424856 34.0 29.0 35.0 13.5 35.0 96-97 29.308843611416883 34.0 27.0 35.0 11.0 35.0 98-99 29.063686320322468 34.0 27.0 35.0 10.0 35.0 100 27.387116026751944 32.0 24.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4224370322299613 1101 2 -0.37884999784925455 1101 3 -0.4321126334427383 1101 4 -0.29073373765949384 1101 5 -0.3765588973556291 1101 6 -0.4301382707160428 1101 7 -0.475024702450618 1101 8 -0.6279958181428995 1101 9 -0.6340771658890887 1101 10-11 -0.6059423219981568 1101 12-13 -0.5923874356254473 1101 14-15 -0.6409939766573984 1101 16-17 -0.6526421634371715 1101 18-19 -0.7048613847381446 1101 20-21 -0.795795415013238 1101 22-23 -0.7774974710220164 1101 24-25 -0.8109837053864268 1101 26-27 -0.8825092462149229 1101 28-29 -0.8415705681584171 1101 30-31 -0.842040783059737 1101 32-33 -0.7796940433364199 1101 34-35 -0.8545283974512827 1101 36-37 -0.9089444563194604 1101 38-39 -1.016807364972415 1101 40-41 -1.0531065951967307 1101 42-43 -0.9551550659767329 1101 44-45 -1.0297580512502478 1101 46-47 -0.8330172723645504 1101 48-49 -0.8404586562367129 1101 50-51 -0.8189414242377424 1101 52-53 -0.6051269983059413 1101 54-55 -0.6383895181976342 1101 56-57 -0.5404800503926737 1101 58-59 -0.638928292033718 1101 60-61 -0.6700389343399706 1101 62-63 -0.7314591018899819 1101 64-65 -0.7669066951119774 1101 66-67 -0.7435873657264906 1101 68-69 -0.771347518180491 1101 70-71 -0.8452885677593756 1101 72-73 -0.8187509092034375 1101 74-75 -0.8928281045556226 1101 76-77 -0.7694562275288952 1101 78-79 -0.7978283559214745 1101 80-81 -1.0034454007589453 1101 82-83 -1.0131306313643478 1101 84-85 -1.0397901354434822 1101 86-87 -1.0689496708822972 1101 88-89 -1.1126683106904132 1101 90-91 -1.0619676802993894 1101 92-93 -1.120523323138542 1101 94-95 -1.2240371656038853 1101 96-97 -1.3108942832862809 1101 98-99 -1.3699893426600909 1101 100 -1.4355969611839257 1102 1 -0.396672979783947 1102 2 -0.42476518379652717 1102 3 -0.3939044005168597 1102 4 -0.24952329334138312 1102 5 -0.31392597048780857 1102 6 -0.30237956355285434 1102 7 -0.35760566439073926 1102 8 -0.29612498775178153 1102 9 -0.4370656142009679 1102 10-11 -0.40145713869809896 1102 12-13 -0.3749194828340521 1102 14-15 -0.47085617226548493 1102 16-17 -0.43904752583085127 1102 18-19 -0.575551944194963 1102 20-21 -0.6578952085050318 1102 22-23 -0.6342581812713632 1102 24-25 -0.7244417661188081 1102 26-27 -0.6780710247768837 1102 28-29 -0.8039816738483339 1102 30-31 -0.7334907793709817 1102 32-33 -0.6802175983524705 1102 34-35 -0.6333443509692671 1102 36-37 -0.6587806632641104 1102 38-39 -0.8459509046010965 1102 40-41 -0.8383015194718908 1102 42-43 -0.933148427460317 1102 44-45 -0.8178398746227984 1102 46-47 -0.6856991201634841 1102 48-49 -0.8530293082657465 1102 50-51 -0.8853404444552027 1102 52-53 -0.6327635426320484 1102 54-55 -0.5880210768168865 1102 56-57 -0.6174482483776842 1102 58-59 -0.6510257296829352 1102 60-61 -0.7916585017204838 1102 62-63 -0.7440296299273044 1102 64-65 -0.6943677601636224 1102 66-67 -0.5757636187092672 1102 68-69 -0.7251693458059663 1102 70-71 -0.765236013871025 1102 72-73 -0.8073994677067553 1102 74-75 -0.8414326088026485 1102 76-77 -0.745768819321512 1102 78-79 -0.848293696827394 1102 80-81 -0.9073993415566228 1102 82-83 -0.9352130908144076 1102 84-85 -0.8903802740104219 1102 86-87 -0.9467309445375918 1102 88-89 -1.109733767776568 1102 90-91 -1.2162913643908162 1102 92-93 -1.053508033100222 1102 94-95 -1.0645919417337453 1102 96-97 -1.1276814595671603 1102 98-99 -1.11217522979911 1102 100 -1.298975326241571 1103 1 -0.47476315837450045 1103 2 -0.4325768982179703 1103 3 -0.5130331084476509 1103 4 -0.3683361002486194 1103 5 -0.31035529346163315 1103 6 -0.3966050138094701 1103 7 -0.45250273509233807 1103 8 -0.4742139062664563 1103 9 -0.5827535715297572 1103 10-11 -0.5683444866541976 1103 12-13 -0.7334697907335936 1103 14-15 -0.750857885968685 1103 16-17 -0.8241698728985938 1103 18-19 -0.7430033021157314 1103 20-21 -0.7721971957707083 1103 22-23 -0.8754728062160737 1103 24-25 -0.8684004583112568 1103 26-27 -0.754281737185984 1103 28-29 -0.8050797039717068 1103 30-31 -0.9602468526217152 1103 32-33 -1.0220009585464211 1103 34-35 -1.06805843345132 1103 36-37 -1.0421339898014352 1103 38-39 -1.0476251674252026 1103 40-41 -0.9725422544489604 1103 42-43 -0.9654940713149927 1103 44-45 -1.1058835186110443 1103 46-47 -1.1511697648056938 1103 48-49 -1.2001703851013943 1103 50-51 -1.1679284639057386 1103 52-53 -0.9537828054120538 1103 54-55 -1.0376722878128106 1103 56-57 -0.966993423988157 1103 58-59 -0.9813730191967096 1103 60-61 -0.9927942081127199 1103 62-63 -0.9982156650103278 1103 64-65 -1.0565120564931405 1103 66-67 -1.0677805969717014 1103 68-69 -1.0108146243646416 1103 70-71 -0.9157012968903686 1103 72-73 -1.1871589318216849 1103 74-75 -1.1080026066479185 1103 76-77 -1.0467063157775485 1103 78-79 -1.1433077655746153 1103 80-81 -1.252624599021626 1103 82-83 -1.3891476147514403 1103 84-85 -1.3239585240926424 1103 86-87 -1.3528643638921025 1103 88-89 -1.4007874810311804 1103 90-91 -1.3426207129441856 1103 92-93 -1.3669266441689807 1103 94-95 -1.6522947351959658 1103 96-97 -1.685667505486844 1103 98-99 -1.755393249388252 1103 100 -1.8012335082292914 1104 1 -0.431117129981331 1104 2 -0.48288383652820244 1104 3 -0.5143129659803307 1104 4 -0.36190219870126583 1104 5 -0.2722121387546963 1104 6 -0.23634395418429932 1104 7 -0.271715153037249 1104 8 -0.3351394790773128 1104 9 -0.48491100721581404 1104 10-11 -0.49396389782678085 1104 12-13 -0.42563635078623463 1104 14-15 -0.6159803616319479 1104 16-17 -0.5316802295439089 1104 18-19 -0.5814938187677043 1104 20-21 -0.6409856853700333 1104 22-23 -0.5362240253464208 1104 24-25 -0.6523287916710814 1104 26-27 -0.684260785389796 1104 28-29 -0.6182568304322515 1104 30-31 -0.7056222297968517 1104 32-33 -0.8050690206948588 1104 34-35 -0.714591541080928 1104 36-37 -0.7835587975598344 1104 38-39 -0.7808203102288829 1104 40-41 -0.8381210707519742 1104 42-43 -0.6817525673111575 1104 44-45 -0.8120227129025679 1104 46-47 -0.8988164487833856 1104 48-49 -0.8527534003253834 1104 50-51 -0.8817465587722424 1104 52-53 -0.8364153197132111 1104 54-55 -0.7665633837275223 1104 56-57 -0.8048023651335541 1104 58-59 -0.850985698396947 1104 60-61 -0.7495249573504168 1104 62-63 -0.8768124817561116 1104 64-65 -0.890633457046718 1104 66-67 -0.7225158496836244 1104 68-69 -0.7228219727821283 1104 70-71 -0.7934688481476613 1104 72-73 -0.8642401986164501 1104 74-75 -0.9548942064820665 1104 76-77 -0.8480022571467671 1104 78-79 -0.9028514127038036 1104 80-81 -0.8884617718867851 1104 82-83 -0.9238057139721967 1104 84-85 -1.092873299328673 1104 86-87 -1.0485172636916396 1104 88-89 -1.0016646922529269 1104 90-91 -0.9256676153685035 1104 92-93 -0.9693280893903662 1104 94-95 -1.0374725930735593 1104 96-97 -1.0942146886138637 1104 98-99 -0.9613742961463494 1104 100 -0.9633080662907183 1105 1 -0.5040740120689122 1105 2 -0.4074151354308988 1105 3 -0.3540776682636526 1105 4 -0.35055218422900936 1105 5 -0.3365330839930891 1105 6 -0.320706621880106 1105 7 -0.3107209442931804 1105 8 -0.32590900309302384 1105 9 -0.5118769397586291 1105 10-11 -0.572329756478716 1105 12-13 -0.5904258794613071 1105 14-15 -0.6633585299384137 1105 16-17 -0.6336264539321306 1105 18-19 -0.6405828666814628 1105 20-21 -0.6379426286411416 1105 22-23 -0.6551827405865325 1105 24-25 -0.6886476849081902 1105 26-27 -0.6763301651535727 1105 28-29 -0.82009027711198 1105 30-31 -0.8410647278731034 1105 32-33 -0.7157916098874892 1105 34-35 -0.7802022820840051 1105 36-37 -0.8169449997337352 1105 38-39 -0.7825196237967944 1105 40-41 -0.7243713090147352 1105 42-43 -0.7759843140301896 1105 44-45 -0.6893622767581675 1105 46-47 -0.8281695977346786 1105 48-49 -0.73966330287886 1105 50-51 -0.726038606561282 1105 52-53 -0.7055319355112744 1105 54-55 -0.6000022978611455 1105 56-57 -0.6695551836938023 1105 58-59 -0.6555243393226036 1105 60-61 -0.5506140829201911 1105 62-63 -0.8037870623548216 1105 64-65 -0.728162607466416 1105 66-67 -0.710565295384427 1105 68-69 -0.7029710370271829 1105 70-71 -0.6918498379558997 1105 72-73 -0.7435741100556541 1105 74-75 -0.763266054059784 1105 76-77 -0.6661210520195873 1105 78-79 -0.664443513910765 1105 80-81 -0.6394662994676175 1105 82-83 -0.6605879547481912 1105 84-85 -0.6570079289683264 1105 86-87 -0.6790816111105187 1105 88-89 -0.7360201238203317 1105 90-91 -0.7953254700388044 1105 92-93 -0.9199821059711013 1105 94-95 -0.7983869102536616 1105 96-97 -0.9878324233644591 1105 98-99 -0.9544508508550074 1105 100 -1.139973258277557 1106 1 -0.475005346835097 1106 2 -0.5237432404811209 1106 3 -0.4141119124334871 1106 4 -0.38801075329396184 1106 5 -0.3422568312454146 1106 6 -0.43835532513794107 1106 7 -0.36567435629469003 1106 8 -0.3223195553809859 1106 9 -0.36928498710329904 1106 10-11 -0.5188983271575509 1106 12-13 -0.5567117754066686 1106 14-15 -0.639313494050981 1106 16-17 -0.6951306461824842 1106 18-19 -0.7086175774008083 1106 20-21 -0.6436982074432578 1106 22-23 -0.6394471780737092 1106 24-25 -0.6477563004988838 1106 26-27 -0.6541178219357064 1106 28-29 -0.6737602609941789 1106 30-31 -0.7181528070634258 1106 32-33 -0.674116740625891 1106 34-35 -0.701881752237874 1106 36-37 -0.7076894304458534 1106 38-39 -0.6955211498318334 1106 40-41 -0.8236714436469228 1106 42-43 -0.6610884818959235 1106 44-45 -0.7597748101196302 1106 46-47 -0.7770190575262603 1106 48-49 -0.7053209955542812 1106 50-51 -0.7191817266122698 1106 52-53 -0.5808766794176421 1106 54-55 -0.6248869349215767 1106 56-57 -0.5457247288644211 1106 58-59 -0.5685308280254588 1106 60-61 -0.6571598300254564 1106 62-63 -0.5323237002337038 1106 64-65 -0.48645707537797733 1106 66-67 -0.5579121650745194 1106 68-69 -0.4864646106953714 1106 70-71 -0.6589051236023593 1106 72-73 -0.5107002179639011 1106 74-75 -0.5979909801365899 1106 76-77 -0.5440167540086236 1106 78-79 -0.560310445581937 1106 80-81 -0.6089731723807965 1106 82-83 -0.5720940733358688 1106 84-85 -0.499193536033566 1106 86-87 -0.5211113199305331 1106 88-89 -0.6155686878415629 1106 90-91 -0.4784929876219195 1106 92-93 -0.553652268391879 1106 94-95 -0.5976016576344847 1106 96-97 -0.7937366120440323 1106 98-99 -0.6254155500186762 1106 100 -0.592636043766035 1107 1 -0.46721887239752036 1107 2 -0.506353672678209 1107 3 -0.45879417777561926 1107 4 -0.2978966140558583 1107 5 -0.29826339538518454 1107 6 -0.3212465952372412 1107 7 -0.2699424942651092 1107 8 -0.3532946928815761 1107 9 -0.4504047884425617 1107 10-11 -0.4299751683051838 1107 12-13 -0.43936668244845833 1107 14-15 -0.5338806168179531 1107 16-17 -0.654508519320153 1107 18-19 -0.570758582918188 1107 20-21 -0.5348105705818043 1107 22-23 -0.6549342644504179 1107 24-25 -0.6336531439426096 1107 26-27 -0.6411612673951765 1107 28-29 -0.595318115435056 1107 30-31 -0.5323492546713808 1107 32-33 -0.6675453652533747 1107 34-35 -0.7167108810422391 1107 36-37 -0.6302202324112329 1107 38-39 -0.8149549204207389 1107 40-41 -0.8841991989407276 1107 42-43 -0.8447073643134999 1107 44-45 -0.7335828891054064 1107 46-47 -0.7073551464991041 1107 48-49 -0.7237153475384375 1107 50-51 -0.7276816315846659 1107 52-53 -0.6454722911686517 1107 54-55 -0.5946228160878775 1107 56-57 -0.5940518090281586 1107 58-59 -0.5199027467435826 1107 60-61 -0.6613526212416261 1107 62-63 -0.5875381104692892 1107 64-65 -0.504173692472726 1107 66-67 -0.48888402243472484 1107 68-69 -0.4304600725246672 1107 70-71 -0.4551953812959795 1107 72-73 -0.3630229914962584 1107 74-75 -0.37001157555925346 1107 76-77 -0.5168687685454358 1107 78-79 -0.5315469909766684 1107 80-81 -0.5599278379463648 1107 82-83 -0.5348726802602464 1107 84-85 -0.622498837029859 1107 86-87 -0.6770742774980576 1107 88-89 -0.5633030273418846 1107 90-91 -0.6271918813463344 1107 92-93 -0.6097595521365662 1107 94-95 -0.49849800423341506 1107 96-97 -0.5242016877139761 1107 98-99 -0.6744227769397497 1107 100 -0.7484832316727363 1108 1 -0.5859051869711109 1108 2 -0.509541900786548 1108 3 -0.5608613965402682 1108 4 -0.4864821114902398 1108 5 -0.4917709832820307 1108 6 -0.4222018064379398 1108 7 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0.49303321477805184 2311 10-11 0.5394849916920563 2311 12-13 0.6114564465396342 2311 14-15 0.7985684475684494 2311 16-17 0.732746920844491 2311 18-19 0.6614823042139975 2311 20-21 0.6888282042078586 2311 22-23 0.8054824560366285 2311 24-25 0.7484651403495945 2311 26-27 0.8312310272477674 2311 28-29 0.8509589131629482 2311 30-31 0.840355844056738 2311 32-33 0.8967969065697048 2311 34-35 0.8990707631920927 2311 36-37 0.9353050115027628 2311 38-39 0.974520718319603 2311 40-41 0.9536378243182853 2311 42-43 1.0566775418667973 2311 44-45 1.0934114791293723 2311 46-47 1.024807162869088 2311 48-49 0.9247320419575473 2311 50-51 0.9965513202697664 2311 52-53 0.919261254862846 2311 54-55 0.8377529449323831 2311 56-57 0.7931751063317449 2311 58-59 0.6783177360037556 2311 60-61 0.7329398344319031 2311 62-63 0.754825207277662 2311 64-65 0.6966979825038138 2311 66-67 0.7633732045667543 2311 68-69 0.715444841922448 2311 70-71 0.7172010036963172 2311 72-73 0.6042725125639521 2311 74-75 0.6801457952502972 2311 76-77 0.6500083358316715 2311 78-79 0.7333408042819727 2311 80-81 0.7872308762200184 2311 82-83 0.8127268228699478 2311 84-85 0.8560903368917963 2311 86-87 0.9008797082244655 2311 88-89 0.9637976899762251 2311 90-91 0.9755679204224172 2311 92-93 0.9314060467336382 2311 94-95 1.0290897875807161 2311 96-97 1.0571402913262133 2311 98-99 1.0283277677225584 2311 100 1.2821427209481016 2312 1 0.4242765229192216 2312 2 0.4775646003819851 2312 3 0.4884552240132294 2312 4 0.33683681920808084 2312 5 0.3138413154381752 2312 6 0.33467788293658174 2312 7 0.38250164903716666 2312 8 0.3930597911271434 2312 9 0.555614603476009 2312 10-11 0.5346724355304033 2312 12-13 0.5163889428713517 2312 14-15 0.7499442557681704 2312 16-17 0.7300539847416232 2312 18-19 0.6961471257722778 2312 20-21 0.7124546912222343 2312 22-23 0.7192741131568283 2312 24-25 0.7797592673638931 2312 26-27 0.8242097032870248 2312 28-29 0.8122063356172049 2312 30-31 0.8415977376855466 2312 32-33 0.8473005943490932 2312 34-35 0.8646728161290795 2312 36-37 0.8435949998195085 2312 38-39 0.9155793157447363 2312 40-41 0.9363233791751213 2312 42-43 0.9576032240531731 2312 44-45 0.8454258646375976 2312 46-47 0.879066897420465 2312 48-49 0.8699635214078683 2312 50-51 0.8668139577591845 2312 52-53 0.8667752536376554 2312 54-55 0.8114206239306512 2312 56-57 0.7564641928121674 2312 58-59 0.7060483908019322 2312 60-61 0.6896630649233941 2312 62-63 0.8027612613943447 2312 64-65 0.7884554827759871 2312 66-67 0.7139212529441821 2312 68-69 0.8399151552577564 2312 70-71 0.7808156043453138 2312 72-73 0.724518778880288 2312 74-75 0.8267442067184021 2312 76-77 0.8589818714038344 2312 78-79 0.8334941570819723 2312 80-81 0.8385396763552144 2312 82-83 0.8450321643409318 2312 84-85 0.8648946562815993 2312 86-87 0.9187181730877896 2312 88-89 0.977160751409965 2312 90-91 0.9596401298889354 2312 92-93 1.0562195656665132 2312 94-95 1.0509174157836583 2312 96-97 1.0050143137275036 2312 98-99 1.0465939954955452 2312 100 1.1288768547221153 2313 1 0.5044688920502516 2313 2 0.523204047225061 2313 3 0.5566729761594864 2313 4 0.44225704347896055 2313 5 0.46422089522646814 2313 6 0.33731452209332247 2313 7 0.39492183909860756 2313 8 0.38619934958575186 2313 9 0.5677909594984456 2313 10-11 0.5119643662974696 2313 12-13 0.5279408897071818 2313 14-15 0.722271778844565 2313 16-17 0.6923097085891428 2313 18-19 0.6166140903767214 2313 20-21 0.6165311417748072 2313 22-23 0.6375153963701834 2313 24-25 0.5966405998542967 2313 26-27 0.6410233899993258 2313 28-29 0.7001377103282422 2313 30-31 0.7946917879301267 2313 32-33 0.8347371691843648 2313 34-35 0.8553167285598775 2313 36-37 0.8116998414984309 2313 38-39 0.8577633389329336 2313 40-41 0.8759139598677379 2313 42-43 0.6603498552451086 2313 44-45 0.8031706780908294 2313 46-47 0.7773108516756864 2313 48-49 0.8932193474971442 2313 50-51 0.8138454387513931 2313 52-53 0.7180949357952642 2313 54-55 0.6047161348988297 2313 56-57 0.7073287735020841 2313 58-59 0.7282851042530609 2313 60-61 0.754235598688787 2313 62-63 0.7261119268645118 2313 64-65 0.7910347776402631 2313 66-67 0.7429189493155235 2313 68-69 0.8326070146295308 2313 70-71 0.8302177026400415 2313 72-73 0.8371630117587969 2313 74-75 0.9109081367912211 2313 76-77 0.860653823782755 2313 78-79 0.8532154912654448 2313 80-81 0.8990797486511575 2313 82-83 0.8608466842559466 2313 84-85 0.9177315366052028 2313 86-87 0.8431458946368906 2313 88-89 0.8787839988286485 2313 90-91 0.9095227644639294 2313 92-93 0.8925747067746386 2313 94-95 0.9765513388657716 2313 96-97 0.9840097004787118 2313 98-99 1.0840300774829998 2313 100 1.0666135545474518 2314 1 0.3317378850002335 2314 2 0.2854730784233226 2314 3 0.35775189230802695 2314 4 0.24826424081624054 2314 5 0.20680128788655594 2314 6 0.30974567730142155 2314 7 0.35226922856960385 2314 8 0.36746347742825947 2314 9 0.4363506681497995 2314 10-11 0.4429415643590815 2314 12-13 0.43739483133474977 2314 14-15 0.49941236190127825 2314 16-17 0.5246595915183079 2314 18-19 0.5597755237581268 2314 20-21 0.5737397943180653 2314 22-23 0.5918312165195445 2314 24-25 0.6679842303067005 2314 26-27 0.702477480374256 2314 28-29 0.6110324224144321 2314 30-31 0.6113476346658828 2314 32-33 0.6127911174074754 2314 34-35 0.6989735155443952 2314 36-37 0.7519291510753305 2314 38-39 0.7125586460561593 2314 40-41 0.8246437931898143 2314 42-43 0.649667441220366 2314 44-45 0.7122242023620018 2314 46-47 0.5985756888498557 2314 48-49 0.5238247100957452 2314 50-51 0.6574589813934466 2314 52-53 0.6213523639306899 2314 54-55 0.651785378149917 2314 56-57 0.6401254182535183 2314 58-59 0.6671470654204157 2314 60-61 0.38896364960459806 2314 62-63 0.5771343069372321 2314 64-65 0.6302192797463491 2314 66-67 0.5655549414262069 2314 68-69 0.6596692655833891 2314 70-71 0.6113675834316936 2314 72-73 0.724840709313618 2314 74-75 0.7597264279439031 2314 76-77 0.7509957791070789 2314 78-79 0.6654011942941978 2314 80-81 0.7106634897007389 2314 82-83 0.709912783974584 2314 84-85 0.7824712673366108 2314 86-87 0.8025651328168806 2314 88-89 0.7520761904376521 2314 90-91 0.6879682725479874 2314 92-93 0.8679476485095563 2314 94-95 0.8501320945001751 2314 96-97 0.920460611122575 2314 98-99 0.9441147551395233 2314 100 0.8893922365177254 2315 1 0.3597490374594017 2315 2 0.4041064616368182 2315 3 0.36957195915215024 2315 4 0.2317955611435849 2315 5 0.24621086985725782 2315 6 0.24086648643271502 2315 7 0.2716961732388157 2315 8 0.18894998867125423 2315 9 0.09382625272328937 2315 10-11 0.2894470214970468 2315 12-13 0.2899145339139011 2315 14-15 0.46464567124324674 2315 16-17 0.3311261364988738 2315 18-19 0.4394631224108636 2315 20-21 0.3327711340035151 2315 22-23 0.4268720719815917 2315 24-25 0.5364570546235257 2315 26-27 0.6680072483245638 2315 28-29 0.5406018416030705 2315 30-31 0.5721511380323108 2315 32-33 0.6108091331265371 2315 34-35 0.5482434519470942 2315 36-37 0.6263851272279055 2315 38-39 0.6733500848843263 2315 40-41 0.738282739069426 2315 42-43 0.717323159952258 2315 44-45 0.6443393305215821 2315 46-47 0.6596696514587777 2315 48-49 0.5395707787654089 2315 50-51 0.47188347860655 2315 52-53 0.25064191507905065 2315 54-55 0.42175395295249274 2315 56-57 0.3561067381074281 2315 58-59 0.39975675556248547 2315 60-61 0.5470930590333865 2315 62-63 0.5990218288083184 2315 64-65 0.5037717682200551 2315 66-67 0.4235535529806427 2315 68-69 0.5053176286759822 2315 70-71 0.4550270891124626 2315 72-73 0.5734570036570545 2315 74-75 0.29381096036134124 2315 76-77 0.4839233332731112 2315 78-79 0.5675992471435336 2315 80-81 0.5069348966244185 2315 82-83 0.5284057908015392 2315 84-85 0.5407163281603431 2315 86-87 0.5925854271981947 2315 88-89 0.504328100482553 2315 90-91 0.6305965490252348 2315 92-93 0.8582458952287055 2315 94-95 0.7534738655765807 2315 96-97 0.7696848119575073 2315 98-99 0.9192436912362609 2315 100 1.0868546912176704 2316 1 0.02542713513864925 2316 2 0.11183114788987325 2316 3 0.12487549968760803 2316 4 -0.004926565843142328 2316 5 -0.04499945334787725 2316 6 -0.2209774295000031 2316 7 -0.11036326784801531 2316 8 -0.11578527538159022 2316 9 -0.03185676956985617 2316 10-11 -0.005465468381935068 2316 12-13 0.03400004881473251 2316 14-15 -0.04225494583662481 2316 16-17 0.00534017578579693 2316 18-19 0.1309723379796921 2316 20-21 0.15902016320048773 2316 22-23 0.10951331772882611 2316 24-25 0.10003655093627373 2316 26-27 -0.03950058153620262 2316 28-29 0.07535244131135954 2316 30-31 -0.5141414097797181 2316 32-33 -0.34191991257808496 2316 34-35 -0.4390664773410151 2316 36-37 -0.2307913776163133 2316 38-39 -0.09359092548898928 2316 40-41 0.06612765421210298 2316 42-43 0.1330531602938052 2316 44-45 -0.0827133862870042 2316 46-47 -0.18813924137595706 2316 48-49 -0.09590082519502374 2316 50-51 0.10148896554022002 2316 52-53 -0.22065204322038312 2316 54-55 -0.0829014368097134 2316 56-57 -0.09514209575695531 2316 58-59 -0.5403945077706354 2316 60-61 -0.012019738808831448 2316 62-63 -0.015535439249838134 2316 64-65 -0.22857898260497223 2316 66-67 -0.5170476264413182 2316 68-69 -0.5064767397474981 2316 70-71 -0.30260721581637995 2316 72-73 -0.5460240411558175 2316 74-75 -0.3273976989821037 2316 76-77 -0.06134631761432985 2316 78-79 -0.03206350089073595 2316 80-81 0.030515317883541115 2316 82-83 0.11883046024483335 2316 84-85 0.16961216245638866 2316 86-87 0.32099775191881363 2316 88-89 0.4552522171531912 2316 90-91 -0.27024389832139306 2316 92-93 -0.33219194893338155 2316 94-95 0.2017085499938851 2316 96-97 0.40705583012105606 2316 98-99 0.3852661993559572 2316 100 0.33556748658939384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 127.0 1 342.0 2 223.0 3 187.0 4 143.0 5 139.0 6 182.0 7 461.0 8 1271.0 9 2361.0 10 3572.0 11 5643.0 12 8160.0 13 10560.0 14 12244.0 15 14400.0 16 17468.0 17 21817.0 18 27087.0 19 32005.0 20 37430.0 21 44285.0 22 49491.0 23 51341.0 24 51348.0 25 52060.0 26 56289.0 27 63304.0 28 73750.0 29 87943.0 30 105234.0 31 129587.0 32 162111.0 33 210683.0 34 292920.0 35 404426.0 36 587031.0 37 850145.0 38 764235.0 39 171080.0 40 184.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.39580189052131 17.68170348636991 12.275622124714799 23.646872498393975 2 16.57816502865125 19.865487816966667 40.097471542001976 23.458875612380105 3 19.22050499748212 26.2298816790486 29.31018778575983 25.239425537709455 4 12.915636750527318 16.04540822839584 37.10255761435306 33.936397406723785 5 13.359644918532517 36.86850491894138 33.820374025808825 15.951476136717268 6 30.571635204278124 35.47927249526888 19.118913764282812 14.830178536170177 7 27.80915208925649 32.18824859542679 22.187064816335877 17.81553449898084 8 26.74981517756812 34.21714125250311 20.50629305044937 18.5267505194794 9 27.7409948121176 15.982726141783946 20.31841093953468 35.95786810656377 10-11 25.587950272473144 25.483875108440028 28.089082524380476 20.839092094706356 12-13 25.870454955942506 23.51513698886891 28.14057730820479 22.473830746983793 14-15 23.410969119286573 24.76498841682176 26.32889638067566 25.495146083216007 16-17 22.643333392024346 27.256185701392628 26.990565325084788 23.109915581498246 18-19 22.34611049207997 26.47080039117669 29.107505773763876 22.075583342979467 20-21 23.316406119972093 25.90152208590651 28.84177666098589 21.940295133135514 22-23 23.094456231236986 25.68254049402282 28.743380224886323 22.479623049853874 24-25 22.637638319527184 25.60523794714124 29.059295973307115 22.69782776002446 26-27 22.475387130750153 25.892886156461152 28.862109986149758 22.769616726638937 28-29 22.37582986013871 26.369701662191325 28.8548029495787 22.399665528091266 30-31 23.2390184294139 25.90881189759945 28.903593076214424 21.94857659677223 32-33 22.989934549179114 25.994471789620366 28.668182071725866 22.347411589474657 34-35 23.02810052255286 26.18775208429827 28.65769112225888 22.12645627088999 36-37 22.924511979820878 26.16810793795265 28.497956493422915 22.40942358880356 38-39 22.897506927725352 26.410129520516882 28.381509312553337 22.310854239204428 40-41 22.944040433282563 25.913887942707703 28.705696849000766 22.43637477500897 42-43 22.710438048998522 26.11809051934773 28.341517857396468 22.82995357425727 44-45 22.375060418076043 26.46587830214469 28.476166929548263 22.682894350231003 46-47 22.41996634946202 26.14457960006581 28.16546393111366 23.269990119358514 48-49 22.645802861217355 26.271983242054752 28.13610694779583 22.94610694893206 50-51 22.62140486801072 26.51683371159686 28.003411643124043 22.858349777268376 52-53 22.581972745366016 26.761443096645642 27.63498352204991 23.02160063593843 54-55 22.395278359241196 26.47903181370196 28.013815002797653 23.11187482425919 56-57 22.62939029202281 26.496434117087936 27.677763844716086 23.19641174617317 58-59 22.432203762443933 26.59074943826697 27.970629573862283 23.006417225426816 60-61 22.860535532430916 26.69528919960064 27.759153552834103 22.685021715134337 62-63 22.782669377118676 26.622885413414377 27.732323321839818 22.862121887627133 64-65 22.50950615269742 26.336280804956512 27.781393254096837 23.372819788249227 66-67 22.466310963122844 26.368877897826366 27.621221284179697 23.54358985487109 68-69 22.156833649911682 26.471328090683734 27.812387162863374 23.55945109654121 70-71 22.329202363008648 26.167705260641526 27.924429989040284 23.578662387309542 72-73 22.293158780089158 26.59781948578223 27.531755847399225 23.577265886729382 74-75 22.438807198508872 26.61290121012041 27.406147330802526 23.542144260568186 76-77 22.242850433237876 26.323487007635432 27.56258846139601 23.871074097730684 78-79 22.12913471071995 26.303244729697518 27.750752819194542 23.81686774038799 80-81 22.23840745215456 26.48529563654407 27.492193688116352 23.784103223185017 82-83 22.498374774962286 26.397256124786377 27.665397464944334 23.438971635307 84-85 22.48787681847723 26.54818822631151 27.28719101225725 23.676743942954012 86-87 22.15537015582417 26.638832847451514 27.597451032054337 23.608345964669976 88-89 22.12749938528064 26.272605423801842 27.680130952267323 23.919764238650192 90-91 22.562442174754228 26.076546705299027 27.644802597025393 23.716208522921352 92-93 22.461944552053517 26.138799755496027 27.602334901578512 23.79692079087194 94-95 22.222892712273172 26.03023069176163 27.67786339594801 24.069013200017185 96-97 22.194387439643062 26.01116302938849 27.607741915542388 24.18670761542606 98-99 22.130102910571722 25.9690199993272 27.51442875495392 24.386448335147158 100 22.28496489939859 25.939720791549203 27.389884668640363 24.385429640411843 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2268.0 1 1659.0 2 3119.5 3 4417.0 4 2702.0 5 1909.5 6 2616.5 7 4624.0 8 7024.5 9 7783.5 10 6460.0 11 5222.0 12 5171.5 13 5469.5 14 5945.0 15 6355.0 16 6691.0 17 7119.0 18 7546.5 19 7870.5 20 8353.5 21 9138.5 22 10155.0 23 11489.0 24 13163.5 25 15328.0 26 18368.0 27 22101.0 28 26119.0 29 31356.5 30 38515.5 31 45704.5 32 53715.0 33 62631.5 34 71911.5 35 81397.5 36 91576.5 37 103287.0 38 111051.0 39 114694.0 40 116921.0 41 119070.5 42 123927.0 43 130909.5 44 137892.0 45 147509.5 46 158263.0 47 166729.5 48 175226.5 49 182921.0 50 186525.5 51 186023.0 52 181160.5 53 174204.0 54 167175.5 55 155875.5 56 141888.0 57 126118.0 58 110963.0 59 97059.0 60 80694.0 61 65173.5 62 52976.5 63 42221.5 64 32106.0 65 24045.0 66 18090.5 67 14018.0 68 10896.0 69 8170.0 70 6417.5 71 5170.0 72 4056.0 73 3141.5 74 2488.5 75 1896.0 76 1356.5 77 931.5 78 641.0 79 444.0 80 295.5 81 188.5 82 131.0 83 98.5 84 80.0 85 60.0 86 42.5 87 38.5 88 32.5 89 22.5 90 18.0 91 13.5 92 10.0 93 9.0 94 8.5 95 7.5 96 7.0 97 7.5 98 4.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024913308725857994 2 0.11030441247173407 3 0.017078220749175217 4 0.01832729274545798 5 0.02041664953924005 6 0.04741931505888012 7 0.047918943857393224 8 0.039675068681926995 9 0.0334524191004456 10-11 0.03730183188898975 12-13 0.03710879348956423 14-15 0.03205572950460215 16-17 0.03830108948601596 18-19 0.03809669588662423 20-21 0.049292923053304266 22-23 0.04897497745425047 24-25 0.06802900299754569 26-27 0.05959208942265394 28-29 0.05226798544445048 30-31 0.04949731665269599 32-33 0.04599991506310425 34-35 0.04740795985891391 36-37 0.04288859027236355 38-39 0.047987075057190465 40-41 0.05763899502846635 42-43 0.05871773902525601 44-45 0.06090929261873395 46-47 0.06234004781447602 48-49 0.06198803661552361 50-51 0.05579945263394083 52-53 0.06456566700785257 54-55 0.051246017447491854 56-57 0.05599249103336634 58-59 0.054039396639178755 60-61 0.04975848625191875 62-63 0.05738918062920979 64-65 0.055299823835427725 66-67 0.05267677264323392 68-69 0.05832030702643876 70-71 0.05922872302373532 72-73 0.06135214541741602 74-75 0.05227934064441669 76-77 0.053096915041983585 78-79 0.06381622381008291 80-81 0.0641228142091705 82-83 0.05314233584184841 84-85 0.05925143342366773 86-87 0.05694632783052772 88-89 0.06556492460487878 90-91 0.07171944298656294 92-93 0.07586409097422847 94-95 0.0795318205633133 96-97 0.07491025417706708 98-99 0.08450539814851193 100 0.13094816601029827 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4403269.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.709876725110966 #Duplication Level Percentage of deduplicated Percentage of total 1 78.3108807959032 49.1086568094595 2 13.480585378045218 16.90731694479098 3 3.8256667111712064 7.197212635467213 4 1.544303132172589 3.873722361789832 5 0.7987460718434832 2.504463384998573 6 0.4686804396917101 1.7634535557922766 7 0.3199793086025587 1.4046104097936887 8 0.21477387575923476 1.0774754618108726 9 0.1641595104505425 0.9264980397247276 >10 0.8101753339075489 8.888214698858793 >50 0.040695630751668314 1.7538728598569358 >100 0.01977574023202018 2.2235657852403325 >500 9.809633645207145E-4 0.4108739957525664 >1k 4.2650578881112415E-4 0.5814875131669465 >5k 4.265057888111237E-5 0.18428080427396437 >10k+ 1.279517366433371E-4 1.1942947392228347 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19491 0.44264840508267833 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14793 0.33595494620019806 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10369 0.23548413689919923 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6890 0.15647465553433143 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.271039993241385E-5 2 0.0 0.0 0.0 0.0 2.271039993241385E-5 3 0.0 0.0 0.0 0.0 2.271039993241385E-5 4 0.0 0.0 0.0 0.0 2.271039993241385E-5 5 0.0 0.0 0.0 0.0 2.271039993241385E-5 6 0.0 0.0 0.0 0.0 2.271039993241385E-5 7 0.0 0.0 0.0 0.0 2.271039993241385E-5 8 0.0 0.0 0.0 0.0 2.271039993241385E-5 9 0.0 0.0 0.0 4.54207998648277E-5 2.271039993241385E-5 10-11 0.0 0.0 0.0 6.813119979724155E-5 2.271039993241385E-5 12-13 0.0 0.0 0.0 6.813119979724155E-5 2.271039993241385E-5 14-15 0.0 0.0 0.0 7.948639976344848E-5 3.4065599898620776E-5 16-17 0.0 0.0 0.0 9.08415997296554E-5 4.54207998648277E-5 18-19 0.0 0.0 0.0 9.08415997296554E-5 9.08415997296554E-5 20-21 0.0 0.0 0.0 1.816831994593108E-4 1.0219679969586233E-4 22-23 0.0 0.0 0.0 4.655631986144839E-4 1.4761759956069003E-4 24-25 0.0 1.1355199966206925E-5 0.0 0.0013853343958772448 1.816831994593108E-4 26-27 0.0 2.271039993241385E-5 0.0 0.0032475871903351805 1.816831994593108E-4 28-29 0.0 2.271039993241385E-5 0.0 0.007653404777223468 1.816831994593108E-4 30-31 0.0 2.271039993241385E-5 0.0 0.02434554872754765 1.816831994593108E-4 32-33 0.0 2.271039993241385E-5 0.0 0.06540595180535189 2.271039993241385E-4 34-35 0.0 2.271039993241385E-5 0.0 0.123964718031081 2.271039993241385E-4 36-37 0.0 2.271039993241385E-5 0.0 0.21519239455958744 2.271039993241385E-4 38-39 0.0 2.271039993241385E-5 0.0 0.3542935941456222 2.271039993241385E-4 40-41 0.0 3.4065599898620776E-5 0.0 0.5156850512653213 2.271039993241385E-4 42-43 0.0 5.6775999831034626E-5 0.0 0.6684011356108382 2.271039993241385E-4 44-45 0.0 6.813119979724155E-5 0.0 0.8459169766825511 2.3845919929034543E-4 46-47 0.0 6.813119979724155E-5 0.0 1.0530585344660979 2.4981439925655236E-4 48-49 0.0 6.813119979724155E-5 0.0 1.268182797825888 2.9523519912138006E-4 50-51 0.0 6.813119979724155E-5 0.0 1.4953889939497222 2.9523519912138006E-4 52-53 0.0 6.813119979724155E-5 0.0 1.7281819484569305 3.747215988848285E-4 54-55 0.0 6.813119979724155E-5 0.0 1.9970730836567105 4.7691839858069086E-4 56-57 0.0 6.813119979724155E-5 0.0 2.2909229483822133 4.882735985468978E-4 58-59 0.0 6.813119979724155E-5 0.0 2.614341753819719 4.996287985131047E-4 60-61 0.0 6.813119979724155E-5 0.0 2.931413002476115 5.223391984455185E-4 62-63 0.0 6.813119979724155E-5 0.0 3.2604753422968256 5.223391984455185E-4 64-65 0.0 6.813119979724155E-5 0.0 3.5873801941239565 5.223391984455185E-4 66-67 0.0 6.813119979724155E-5 0.0 3.947385453852581 5.450495983779324E-4 68-69 0.0 6.813119979724155E-5 0.0 4.345952972666444 5.450495983779324E-4 70-71 0.0 1.0219679969586233E-4 0.0 4.767537481811809 5.450495983779324E-4 72-73 0.0 1.362623995944831E-4 0.0 5.16820571261942 5.904703982427601E-4 74-75 0.0 1.362623995944831E-4 0.0 5.569736938624462 5.904703982427601E-4 76-77 0.0 1.362623995944831E-4 0.0 6.013600350103525 5.904703982427601E-4 78-79 0.0 1.362623995944831E-4 0.0 6.501465161451639 5.904703982427601E-4 80-81 0.0 1.362623995944831E-4 0.0 6.983550176016955 5.904703982427601E-4 82-83 0.0 1.362623995944831E-4 0.0 7.464987944184196 6.358911981075878E-4 84-85 0.0 1.362623995944831E-4 2.271039993241385E-5 7.957099600319672 6.358911981075878E-4 86-87 0.0 1.362623995944831E-4 2.271039993241385E-5 8.470229731592596 6.358911981075878E-4 88 0.0 1.362623995944831E-4 2.271039993241385E-5 8.866276396013962 6.358911981075878E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7715 0.0 51.16057 1 TACCTGG 4910 0.0 48.289574 2 GTATCAA 14085 0.0 42.986393 1 ACCTGGG 5965 0.0 38.839767 3 GTACCTG 6495 0.0 36.940292 1 TAACGCA 580 0.0 36.459843 4 ATAACGC 555 0.0 36.409576 3 ATCAACG 18325 0.0 32.671238 3 TATCAAC 18395 0.0 32.556145 2 CAACGCA 18515 0.0 32.411007 5 TCAACGC 18810 0.0 31.978012 4 GTACATG 17665 0.0 31.744751 1 AACGCAG 19635 0.0 30.992336 6 TACATGG 18080 0.0 30.391266 2 ACATGGG 18855 0.0 28.163853 3 TATAACG 705 0.0 28.02622 2 ACGCAGA 21830 0.0 27.596546 7 CCTGGGG 6210 0.0 26.788101 4 CATGGGG 13365 0.0 26.722366 4 CGCAGAG 22630 0.0 26.57763 8 >>END_MODULE