FastQCFastQC Report
Mon 6 Jun 2016
ERR1211352_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211352_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4403269
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169620.38521380365360375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139040.3157654006602822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98640.2240153849333302No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55750.1266104796232072No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81350.050.41411
TACCTGG52350.047.8562622
GTATCAA139800.040.647621
ACCTGGG60200.040.6041373
GTACCTG63900.039.2137641
TATCAAC180750.031.1989822
ATCAACG179750.030.9560013
CAACGCA180650.030.8221475
TCAACGC184100.030.3519784
AACGCAG191750.029.453466
GTACATG173050.029.0955281
CCTGGGG61750.028.7199654
TACATGG175950.028.1304972
ACATGGG180950.026.3688643
ACGCAGA216550.025.8854057
CGCAGAG222200.025.1849788
TGGGGGG82300.024.6793236
CATGGGG129300.024.311144
ATGGGGG91000.023.4026265
GTGGTAT39350.023.3727321
Produced by FastQC (version 0.11.5)