##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211352_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4403269 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.130044519196986 33.0 31.0 34.0 30.0 34.0 2 32.246380813890774 34.0 31.0 34.0 30.0 34.0 3 32.31593164078779 34.0 31.0 34.0 30.0 34.0 4 35.956309278402024 37.0 35.0 37.0 35.0 37.0 5 35.86503004926567 37.0 35.0 37.0 35.0 37.0 6 35.88355355986654 37.0 35.0 37.0 35.0 37.0 7 35.80183609041374 37.0 35.0 37.0 35.0 37.0 8 35.78150074410625 37.0 35.0 37.0 33.0 37.0 9 37.40045043807226 39.0 37.0 39.0 34.0 39.0 10-11 37.34477237252595 39.0 37.0 39.0 34.0 39.0 12-13 37.267433127524114 39.0 37.0 39.0 34.0 39.0 14-15 38.50078248682967 40.0 38.0 41.0 34.0 41.0 16-17 38.4833001345137 40.0 38.0 41.0 34.0 41.0 18-19 38.48816152272323 40.0 38.0 41.0 34.0 41.0 20-21 38.44999181290083 40.0 38.0 41.0 34.0 41.0 22-23 38.333773498734686 40.0 38.0 41.0 34.0 41.0 24-25 38.24429395524098 40.0 38.0 41.0 34.0 41.0 26-27 38.14303566282233 40.0 37.5 41.0 33.0 41.0 28-29 38.02084496768197 40.0 37.0 41.0 33.0 41.0 30-31 38.0099812207703 40.0 37.0 41.0 33.0 41.0 32-33 37.91712225621464 40.0 37.0 41.0 33.0 41.0 34-35 37.76360426764751 40.0 37.0 41.0 33.0 41.0 36-37 37.602979854285536 40.0 37.0 41.0 33.0 41.0 38-39 37.48124086899983 40.0 36.0 41.0 32.0 41.0 40-41 37.36087699843003 40.0 36.0 41.0 32.0 41.0 42-43 37.2440536792097 40.0 36.0 41.0 31.5 41.0 44-45 37.079692496642835 39.5 36.0 41.0 31.0 41.0 46-47 36.8277553563046 39.0 35.0 41.0 30.5 41.0 48-49 35.276161188426144 39.0 35.0 41.0 24.0 41.0 50-51 35.99775087554269 39.0 35.0 40.0 28.0 41.0 52-53 36.09103986152107 39.0 35.0 40.0 29.0 41.0 54-55 35.91966468548708 38.0 35.0 40.0 28.0 41.0 56-57 35.708354293139934 38.0 34.5 40.0 28.0 41.0 58-59 35.472078698803095 38.0 34.0 40.0 28.0 41.0 60-61 35.20751730135043 37.5 34.0 40.0 27.0 41.0 62-63 34.874995145652015 37.0 34.0 40.0 26.5 41.0 64-65 34.53996394496907 36.0 33.5 39.0 26.0 41.0 66-67 34.19743365667644 36.0 33.0 39.0 26.0 40.5 68-69 33.827290020209986 35.0 33.0 39.0 26.0 40.0 70-71 33.45677313832064 35.0 32.5 38.0 25.5 40.0 72-73 33.070428810958404 35.0 32.0 37.0 25.0 39.5 74-75 32.706397110873766 35.0 32.0 37.0 24.5 39.0 76-77 31.773533595153964 34.0 30.5 36.0 23.5 38.0 78-79 32.207790621013615 34.5 31.5 36.0 24.0 37.5 80-81 32.08715354433263 35.0 31.5 35.0 24.0 37.0 82-83 31.819511254025134 34.0 31.0 35.0 24.0 37.0 84-85 31.54515497463362 34.0 31.0 35.0 23.5 36.0 86-87 31.289121559459574 34.0 31.0 35.0 23.0 36.0 88-89 31.075908830462097 34.0 31.0 35.0 21.5 35.5 90-91 30.859405932274406 34.0 30.0 35.0 20.0 35.0 92-93 30.66613600032158 34.0 30.0 35.0 20.0 35.0 94-95 30.470266408888484 34.0 30.0 35.0 19.5 35.0 96-97 30.281346994698712 34.0 29.0 35.0 19.0 35.0 98-99 30.063150127780062 34.0 29.0 35.0 18.5 35.0 100 27.652467291914256 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2943560974791879 1101 2 -0.27273832146573085 1101 3 -0.32701157032002115 1101 4 -0.2354056602879453 1101 5 -0.207430028830359 1101 6 -0.31053458542710644 1101 7 -0.2881964024770838 1101 8 -0.21821647428392055 1101 9 -0.40471695538781916 1101 10-11 -0.31953318089160376 1101 12-13 -0.38677382593352405 1101 14-15 -0.5463916293859299 1101 16-17 -0.5742110330122117 1101 18-19 -0.5373293572534195 1101 20-21 -0.5524913835961982 1101 22-23 -0.597877969971222 1101 24-25 -0.5049033829813041 1101 26-27 -0.5801906333593436 1101 28-29 -0.546932433160336 1101 30-31 -0.5062580623457364 1101 32-33 -0.5001139784544009 1101 34-35 -0.6644975051360191 1101 36-37 -0.6891619804458671 1101 38-39 -0.6171503063083179 1101 40-41 -0.6539910472397636 1101 42-43 -0.6380219400981346 1101 44-45 -0.6286861562710087 1101 46-47 -0.6668716928627063 1101 48-49 0.5840046918033153 1101 50-51 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1104 48-49 0.6757835934182737 1104 50-51 -0.3072806915320001 1104 52-53 -0.5405943479002033 1104 54-55 -0.5992152470201049 1104 56-57 -0.7263867374875659 1104 58-59 -0.7881548187951282 1104 60-61 -0.7793859507739569 1104 62-63 -0.7597504207989161 1104 64-65 -0.7327740541531469 1104 66-67 -0.8266546850869076 1104 68-69 -0.8692571037149577 1104 70-71 -0.7846229950413957 1104 72-73 -0.7698154978112157 1104 74-75 -0.8072591793039194 1104 76-77 -0.7369876219882379 1104 78-79 -0.6346597574726616 1104 80-81 -0.6157081931732051 1104 82-83 -0.8061262448048403 1104 84-85 -0.7587756531486285 1104 86-87 -0.69954873648458 1104 88-89 -0.6857892179485958 1104 90-91 -0.8611233942205807 1104 92-93 -0.7748082420250029 1104 94-95 -0.8252210725615114 1104 96-97 -0.9296181103245864 1104 98-99 -0.9603649655587141 1104 100 -1.0231882447950582 1105 1 -0.29067360112780705 1105 2 -0.2519613717064395 1105 3 -0.25332836094467837 1105 4 -0.13888880603596476 1105 5 -0.24947013488852576 1105 6 -0.25905324853323464 1105 7 -0.41781320302722236 1105 8 -0.30484699071462273 1105 9 -0.41390856861519865 1105 10-11 -0.4139052484028909 1105 12-13 -0.437380168719919 1105 14-15 -0.6073786890669837 1105 16-17 -0.6102902232064906 1105 18-19 -0.4658784932507771 1105 20-21 -0.5955054128756601 1105 22-23 -0.6250241365672551 1105 24-25 -0.621091229973473 1105 26-27 -0.6911336975667908 1105 28-29 -0.7037753490066905 1105 30-31 -0.6305909644693415 1105 32-33 -0.7153785778569315 1105 34-35 -0.7380409523392046 1105 36-37 -0.7834757446272889 1105 38-39 -0.8024201965381508 1105 40-41 -0.7697228017027342 1105 42-43 -0.7521090017453389 1105 44-45 -0.7921315614948483 1105 46-47 -0.7465391216674959 1105 48-49 0.41477538305990436 1105 50-51 -0.4288842411177143 1105 52-53 -0.5674017327516339 1105 54-55 -0.785630680799521 1105 56-57 -0.794084601476591 1105 58-59 -0.7786514878228559 1105 60-61 -0.8487425354154681 1105 62-63 -0.8316756545317432 1105 64-65 -0.7217697265953831 1105 66-67 -0.8544054728247872 1105 68-69 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0.29840050968473975 2311 5 0.37949964126499225 2311 6 0.3932266362810708 2311 7 0.38881795631557026 2311 8 0.1099007538496295 2311 9 0.3990220022452391 2311 10-11 0.2884951187592648 2311 12-13 0.4158607463802255 2311 14-15 0.3933391873439689 2311 16-17 0.40177002300448095 2311 18-19 0.5942310393265302 2311 20-21 0.6821392705573999 2311 22-23 0.6849512361356531 2311 24-25 0.6292630102147427 2311 26-27 0.6693324774838487 2311 28-29 0.7089689091444171 2311 30-31 0.5885369595378549 2311 32-33 0.5805584412017097 2311 34-35 0.7532326501120608 2311 36-37 0.7521526621617269 2311 38-39 0.7934995609395372 2311 40-41 0.7320225359268093 2311 42-43 0.7433576537623239 2311 44-45 0.7980176746559806 2311 46-47 0.8302842127352434 2311 48-49 -5.931568939565437 2311 50-51 -1.1456049398262138 2311 52-53 0.26436255968241085 2311 54-55 0.701604079801136 2311 56-57 0.8822041857413936 2311 58-59 0.9067664671660722 2311 60-61 0.7135294412312163 2311 62-63 0.6028371814200781 2311 64-65 0.6091367175731861 2311 66-67 0.8051228229941501 2311 68-69 0.8964785679086162 2311 70-71 0.8291993063181096 2311 72-73 0.29370211515627176 2311 74-75 0.5778798916794727 2311 76-77 0.7184594643028994 2311 78-79 0.627421129888603 2311 80-81 0.7180594601503572 2311 82-83 0.7763949470577991 2311 84-85 0.8255585601440778 2311 86-87 0.898972656174152 2311 88-89 0.924939248638541 2311 90-91 0.9459751907575118 2311 92-93 0.9530563013213609 2311 94-95 0.9666999358286148 2311 96-97 0.9978756000329518 2311 98-99 1.0636075785278436 2311 100 1.1528564854885381 2312 1 0.04206648797051571 2312 2 0.16575068356676326 2312 3 0.21747994363290246 2312 4 0.022547421260910028 2312 5 0.21037307381657655 2312 6 0.13818809293361056 2312 7 0.2479880068996536 2312 8 0.3174204033196588 2312 9 0.27367739514527045 2312 10-11 0.18643537738317661 2312 12-13 0.28043230912447115 2312 14-15 0.30303102336899457 2312 16-17 0.41961720155670434 2312 18-19 0.2710139331974517 2312 20-21 0.3224279556800269 2312 22-23 0.5458327039677187 2312 24-25 0.6972232636063822 2312 26-27 0.6042395558428595 2312 28-29 0.7076360397286905 2312 30-31 0.6758356916047177 2312 32-33 0.68122866402841 2312 34-35 0.3372043982608588 2312 36-37 0.5739458621532734 2312 38-39 0.6259838275516074 2312 40-41 0.44294328644963343 2312 42-43 0.5193045819127846 2312 44-45 0.6201827805770606 2312 46-47 0.6034314265484468 2312 48-49 -4.69581643026677 2312 50-51 -0.9119529373433153 2312 52-53 0.3396492380160723 2312 54-55 0.6523417981714559 2312 56-57 0.8323349113865319 2312 58-59 0.9252748501816441 2312 60-61 0.8976428833048828 2312 62-63 0.6178696759643785 2312 64-65 0.8682989278684161 2312 66-67 0.8826444101935991 2312 68-69 0.8214045726436581 2312 70-71 0.8169725383532835 2312 72-73 0.9450466231354966 2312 74-75 0.541517586650798 2312 76-77 0.6915117821847723 2312 78-79 0.3316703430160928 2312 80-81 0.5854317731249381 2312 82-83 0.6269446203655527 2312 84-85 0.6923565414931225 2312 86-87 0.7896202884069226 2312 88-89 0.8478742668621351 2312 90-91 0.7211349010974324 2312 92-93 0.8328014431859074 2312 94-95 0.9578935852758939 2312 96-97 0.9641466774105076 2312 98-99 0.9392286878352856 2312 100 0.4326191562890287 2313 1 0.055283506643263536 2313 2 0.18850513847611694 2313 3 0.2903388532873237 2313 4 0.06333751621818351 2313 5 0.11250984932787844 2313 6 0.25993568875433937 2313 7 0.10791888064537858 2313 8 0.2123458296475107 2313 9 0.45552281449207754 2313 10-11 0.17507185303040984 2313 12-13 0.02417510364114861 2313 14-15 0.1586091618770169 2313 16-17 0.13864638413865293 2313 18-19 0.4699373216479259 2313 20-21 0.38104752568094113 2313 22-23 0.4609457951459177 2313 24-25 0.336392604375078 2313 26-27 0.5036695918439449 2313 28-29 0.3801769755713096 2313 30-31 0.5118275169023505 2313 32-33 0.43445908816163836 2313 34-35 0.3014367510718543 2313 36-37 -0.3682381030528319 2313 38-39 0.21160007382553658 2313 40-41 0.4199335363737262 2313 42-43 0.469423860746943 2313 44-45 0.38263144951108785 2313 46-47 0.2276963730280741 2313 48-49 -5.286060150274324 2313 50-51 -1.3860047319761435 2313 52-53 0.044915809733844014 2313 54-55 0.2264804693107365 2313 56-57 0.23161220322065645 2313 58-59 0.38935326052905594 2313 60-61 0.6148764763529044 2313 62-63 0.631707856826381 2313 64-65 0.7033990271917432 2313 66-67 0.8206632508957128 2313 68-69 0.7912141265854729 2313 70-71 0.7889279130132891 2313 72-73 0.5668041532219164 2313 74-75 0.6716407541508289 2313 76-77 0.6844550519226935 2313 78-79 0.6290141445658008 2313 80-81 -0.17761248789421558 2313 82-83 0.20127186860681334 2313 84-85 0.5201506455257636 2313 86-87 0.6948380005455341 2313 88-89 0.743590510990721 2313 90-91 0.8586821540893546 2313 92-93 0.799464946607138 2313 94-95 0.831731290684715 2313 96-97 0.7752330666740797 2313 98-99 0.8568131691980021 2313 100 1.0972131434297587 2314 1 0.06904944542267089 2314 2 0.19156767243061523 2314 3 0.22536813591108285 2314 4 0.01764956644179705 2314 5 0.1563575629209808 2314 6 0.26291067258849665 2314 7 0.15767073717825753 2314 8 0.24127320195472635 2314 9 0.39172908257559413 2314 10-11 0.2802059319037724 2314 12-13 0.14015031773358544 2314 14-15 0.18223645779382736 2314 16-17 0.40170117034671193 2314 18-19 0.5215458849134791 2314 20-21 0.5538106869035033 2314 22-23 0.2366948546866965 2314 24-25 0.3871470049490142 2314 26-27 0.41954765165568375 2314 28-29 0.5263138283414008 2314 30-31 0.48601035163981976 2314 32-33 0.45927850414304316 2314 34-35 0.37019448696865 2314 36-37 0.43316949205919286 2314 38-39 0.5157756728676475 2314 40-41 0.22103803677565992 2314 42-43 0.2558594885510317 2314 44-45 0.4712019569866541 2314 46-47 0.5767117983564063 2314 48-49 -6.467601016287759 2314 50-51 -1.5771062577968564 2314 52-53 -0.7969723879572612 2314 54-55 -0.2785136574743561 2314 56-57 -0.04156953688410425 2314 58-59 0.4167416979032552 2314 60-61 0.5800531597793963 2314 62-63 0.5314001064409979 2314 64-65 0.7550536164925603 2314 66-67 0.740045310287563 2314 68-69 0.703675297722782 2314 70-71 0.5397001713228988 2314 72-73 0.6730742973901016 2314 74-75 0.7176501907508026 2314 76-77 0.6515589909351114 2314 78-79 0.509235715912574 2314 80-81 0.5033877780868607 2314 82-83 0.5147563476577979 2314 84-85 0.6030834486489205 2314 86-87 0.6135114603011935 2314 88-89 0.46429894738546906 2314 90-91 0.6932618382962197 2314 92-93 0.7602436456335795 2314 94-95 0.8078691247335357 2314 96-97 0.7787720570213459 2314 98-99 0.9109566781197138 2314 100 -0.42769546972692396 2315 1 0.10904638761061136 2315 2 0.20658685267009957 2315 3 0.3040186156720708 2315 4 0.14349008545676867 2315 5 0.19233145686878572 2315 6 0.2791214636160362 2315 7 0.3360948386745122 2315 8 0.26977618542363757 2315 9 0.4438686534896945 2315 10-11 0.2554398112037646 2315 12-13 0.42468192355178047 2315 14-15 0.2010466632671708 2315 16-17 0.3711952358917401 2315 18-19 0.44392504037460156 2315 20-21 0.32055102890245735 2315 22-23 0.43071763641021477 2315 24-25 0.5578004653090218 2315 26-27 0.5499475607548803 2315 28-29 0.644165319798006 2315 30-31 0.6506642085209791 2315 32-33 0.6855954956449679 2315 34-35 0.7544508348625456 2315 36-37 0.6031380156788728 2315 38-39 0.6407421160856615 2315 40-41 0.7227056667165002 2315 42-43 0.7508393610624964 2315 44-45 0.7587544135076101 2315 46-47 0.6787101209468815 2315 48-49 -4.679354903655643 2315 50-51 -0.7843244454465719 2315 52-53 0.3951070342873564 2315 54-55 0.28290535304267195 2315 56-57 0.5902729985173707 2315 58-59 0.8158238643916107 2315 60-61 0.6199242651773602 2315 62-63 0.40103916945631113 2315 64-65 0.4890129994331076 2315 66-67 -0.140153057044067 2315 68-69 0.5372835075460216 2315 70-71 0.5221201100416195 2315 72-73 0.6921257205102407 2315 74-75 0.8117084202812705 2315 76-77 0.9015351086332366 2315 78-79 0.7777322890901601 2315 80-81 0.6410860382086128 2315 82-83 0.6001109999320313 2315 84-85 0.6186763159622934 2315 86-87 0.6215567890381095 2315 88-89 0.6262160368806882 2315 90-91 0.7605719080069484 2315 92-93 0.8100116207993509 2315 94-95 0.46948997497565514 2315 96-97 0.5338919088863676 2315 98-99 0.5684864982765667 2315 100 0.6870498783973389 2316 1 0.19859779010983658 2316 2 0.2271562312006452 2316 3 0.21138691108520646 2316 4 -0.031263834533660884 2316 5 0.15646240129558464 2316 6 0.14547181053045222 2316 7 0.23143168769699685 2316 8 0.2894594980740379 2316 9 0.4301885627482136 2316 10-11 0.3792536865452547 2316 12-13 0.35136677140479833 2316 14-15 0.45391109492705795 2316 16-17 0.4874699611852691 2316 18-19 0.12891662098143541 2316 20-21 0.41548613271558565 2316 22-23 0.5509465818441015 2316 24-25 0.5852545180586475 2316 26-27 0.5906914855203453 2316 28-29 0.5820601939506034 2316 30-31 0.49196651663795166 2316 32-33 0.4823648916326633 2316 34-35 0.393960261316181 2316 36-37 0.28388315857564095 2316 38-39 0.37817238885852333 2316 40-41 -0.012959115604481042 2316 42-43 -0.007392773241207351 2316 44-45 0.2391048096830488 2316 46-47 0.2890875318069348 2316 48-49 -4.978336748290399 2316 50-51 -1.0546533044355613 2316 52-53 0.20910247741964127 2316 54-55 0.5280104669096701 2316 56-57 0.3878783064147484 2316 58-59 0.17799627022771602 2316 60-61 0.35238462549568794 2316 62-63 0.2781720433227193 2316 64-65 0.34194970787564927 2316 66-67 0.352339008917788 2316 68-69 0.1721726482961401 2316 70-71 -0.2409162946717558 2316 72-73 -0.08695932224378566 2316 74-75 0.10368530302150702 2316 76-77 0.13925477130387875 2316 78-79 0.243994386596178 2316 80-81 0.20316871010855664 2316 82-83 0.11219047953919059 2316 84-85 0.2172691250288814 2316 86-87 0.3494383671796655 2316 88-89 0.17843407762104135 2316 90-91 0.2989439927237001 2316 92-93 0.351144908547635 2316 94-95 0.5554572475007973 2316 96-97 0.6053325191813101 2316 98-99 0.5572085148352635 2316 100 -0.18581721053003974 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 7.0 12 28.0 13 116.0 14 474.0 15 1395.0 16 3408.0 17 7042.0 18 11709.0 19 16806.0 20 22416.0 21 29563.0 22 36280.0 23 39223.0 24 40419.0 25 43337.0 26 49719.0 27 59946.0 28 72799.0 29 89008.0 30 108441.0 31 133539.0 32 168659.0 33 219217.0 34 307611.0 35 428959.0 36 656823.0 37 976394.0 38 779057.0 39 100857.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.42340322144254 18.378892402238012 13.09719962171233 24.100504754607115 2 16.226397439848544 20.238591362554807 39.751547575761776 23.783463621834873 3 18.911553378787474 25.98979938981656 29.91032111164758 25.18832611974839 4 12.894069960365847 16.372552850078957 36.90785014256301 33.825527046992185 5 13.24421267592711 37.001061035908975 33.84768139126517 15.907044896898743 6 30.865227629745085 35.677266140224454 18.788381995285775 14.669124234744688 7 27.86487850952486 32.22466409599963 21.99937001321973 17.91108738125578 8 26.861860866105182 34.531467603952514 20.199801592889322 18.40686993705298 9 27.51700107817532 15.926400622875079 20.176550924648332 36.38004737430127 10-11 25.693910664583797 25.5579645279602 27.95573552003883 20.792389287417183 12-13 25.797067811514367 23.490403936716035 28.15763488176044 22.554893370009157 14-15 23.294744909088454 24.774692679645447 26.2526646429404 25.677897768325696 16-17 22.63962317863371 27.362869296532665 26.91862959968124 23.07887792515239 18-19 22.331715368740817 26.482858530787013 29.06748372629517 22.117942374177005 20-21 23.22119799498804 25.928268463484656 28.794175595362127 22.056357946165182 22-23 22.97684249588288 25.722478995389665 28.703600139850945 22.59707836887651 24-25 22.535829086864158 25.682482048552142 29.019573198621206 22.762115665962494 26-27 22.456263146491718 25.918045832343537 28.84519127393167 22.780499747233076 28-29 22.39282470311205 26.371709693716085 28.821576762543128 22.413888840628733 30-31 23.12084609264009 25.919087400555764 28.91019026455668 22.049876242247468 32-33 22.959435365729018 26.031729447971202 28.623187253710665 22.385647932589116 34-35 23.002290546928272 26.18258230318537 28.67781108757367 22.13731606231269 36-37 22.9123268537449 26.169161359890143 28.534611873784943 22.383899912580016 38-39 22.8537332947628 26.386514669693252 28.420803600461532 22.338948435082422 40-41 22.920967654752676 25.931613891981435 28.75578745499151 22.39163099827438 42-43 22.68855221627244 26.14628584795906 28.340806360125892 22.824355575642606 44-45 22.346463878603345 26.44101407053414 28.54056734112534 22.671954709737175 46-47 22.37053814376605 26.131970734747533 28.213981035549484 23.283510085936925 48-49 22.665551937871875 26.324905131583666 28.08549926946492 22.924043661079537 50-51 22.53846996020012 26.501331281178125 28.01747224207194 22.942726516549815 52-53 22.499746449641947 26.684775405490342 27.703519733259345 23.11195841160837 54-55 22.366711785113093 26.398368059092558 28.058290806954716 23.176629348839633 56-57 22.60401953509774 26.426936322422236 27.66020122431635 23.308842918163673 58-59 22.3360054654719 26.527991067732763 28.022524176320974 23.113479290474366 60-61 22.77712355880987 26.677322171360956 27.768839519014684 22.77671475081449 62-63 22.72000481499189 26.660757702567505 27.642005353271635 22.97723212916897 64-65 22.371130976380115 26.347521314908683 27.71448073622893 23.566866972482273 66-67 22.30838386437066 26.380830520689756 27.58361402976717 23.727171585172414 68-69 22.026548405189736 26.519815892650538 27.713137923537882 23.740497778621837 70-71 22.2048720117834 26.205667991178807 27.818434919143577 23.77102507789422 72-73 22.23447726615525 26.601838733616688 27.343751597187723 23.81993240304034 74-75 22.27151835053045 26.632298932287352 27.229897771226497 23.866284945955705 76-77 22.049865717665487 26.423092927373066 27.375039294390852 24.1520020605706 78-79 21.94857549144157 26.361036757375384 27.533969067710334 24.15641868347271 80-81 22.041176596834568 26.553791581107784 27.243115251524674 24.16191657053298 82-83 22.26153554547151 26.430057623072944 27.41341690092827 23.894989930527284 84-85 22.217966803188386 26.611524159346512 27.016012339200813 24.15449669826429 86-87 21.88743003178022 26.6604032263835 27.271520249853115 24.18064649198316 88-89 21.80462785555035 26.33662633074246 27.34288140529494 24.51586440841225 90-91 22.164040322725363 26.16698781863723 27.3422268540592 24.326745004578203 92-93 22.022484313085062 26.256191067600575 27.29083697010526 24.430487649209102 94-95 21.721548598828644 26.11900375027325 27.383796889061752 24.775650761836356 96-97 21.57829932117245 26.111791754298924 27.423411175723324 24.886497748805304 98-99 21.464436044549903 26.10817980458453 27.297451476337052 25.129932674528515 100 21.386331965248555 26.124921452123623 27.398907803989935 25.089838778637883 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2140.0 1 1628.0 2 2750.0 3 3875.5 4 2484.5 5 1702.5 6 2382.5 7 4307.5 8 6311.5 9 6858.0 10 5840.0 11 4767.0 12 4713.0 13 5151.5 14 5628.5 15 5977.5 16 6522.5 17 6969.5 18 7399.0 19 7909.0 20 8402.0 21 9031.0 22 9960.0 23 11391.5 24 13146.5 25 15265.5 26 18200.5 27 21860.0 28 25728.0 29 31049.0 30 38292.0 31 45516.5 32 54212.0 33 63621.5 34 72341.0 35 81385.5 36 91564.5 37 103059.5 38 110696.0 39 114438.5 40 116573.0 41 118260.5 42 122405.0 43 129501.5 44 137189.5 45 147443.0 46 158554.5 47 166609.0 48 174665.0 49 182367.5 50 186850.0 51 187670.0 52 184825.5 53 179003.0 54 171121.5 55 158993.0 56 144548.0 57 129155.5 58 113698.5 59 98116.5 60 80609.5 61 64523.5 62 52124.5 63 40976.5 64 30630.5 65 22666.5 66 16860.0 67 13097.5 68 9982.0 69 7435.0 70 5784.0 71 4526.5 72 3583.0 73 2800.0 74 2188.5 75 1605.5 76 1119.0 77 700.0 78 421.5 79 252.0 80 156.5 81 98.0 82 57.0 83 44.5 84 26.0 85 16.0 86 10.5 87 5.0 88 4.0 89 1.0 90 1.0 91 1.5 92 2.0 93 1.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030159411110245594 2 0.0020893567937820745 3 6.813119979724155E-5 4 0.02282395193207592 5 5.677599983103462E-4 6 0.0 7 4.54207998648277E-5 8 0.004564790386415184 9 0.01044678396891037 10-11 0.0012377167963165548 12-13 1.1355199966206925E-4 14-15 1.2490719962827618E-4 16-17 3.5201119895241466E-4 18-19 0.0 20-21 7.948639976344846E-5 22-23 2.1574879935793155E-4 24-25 6.813119979724155E-5 26-27 6.813119979724155E-5 28-29 0.0011468751965868994 30-31 0.0025094991925317302 32-33 0.009515657571681404 34-35 0.018781500744106252 36-37 0.02174520793528626 38-39 0.021449972736164884 40-41 0.004791894385739322 42-43 0.004803249585705529 44-45 0.008629951974317264 46-47 0.011468751965868995 48-49 1.2044006396157036 50-51 0.016873827149783492 52-53 0.017952571146573146 54-55 0.009390750372053126 56-57 0.007312748778237259 58-59 0.008073547175973124 60-61 0.005087129584860702 62-63 0.008039481576074502 64-65 0.018940473543633148 66-67 0.028876273514064212 68-69 0.02888762871403042 70-71 0.02600340792261386 72-73 0.02271039993241385 74-75 0.009061449573033127 76-77 0.013739791959110379 78-79 0.007483076777730364 80-81 0.0025549199923965583 82-83 0.013751147159076585 84-85 0.023596105529777988 86-87 0.022767175932244883 88-89 0.03186269110517663 90-91 0.020166835139983498 92-93 0.012604271962489687 94-95 0.005825217582664153 96-97 0.0036904399890172507 98-99 0.015908635152655903 100 0.033952047898958705 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4403269.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.787621194660936 #Duplication Level Percentage of deduplicated Percentage of total 1 78.18077504620796 49.087848883063046 2 13.645021762838013 17.134769152759556 3 3.8150572631469544 7.186151108232325 4 1.497268632725236 3.760397429528001 5 0.7959722757218192 2.4988602864736897 6 0.4671438692975893 1.7598511385319142 7 0.32127460875667463 1.412044790385389 8 0.23504211974664704 1.1806188463554088 9 0.16836900685254383 0.9514340480860914 >10 0.8096159086795481 8.845519662341145 >50 0.043451020253419556 1.8427052711693395 >100 0.019555624359132728 2.198369742860099 >500 8.973555970378012E-4 0.38170920869892133 >1k 3.845809505249771E-4 0.5173612410944882 >5k 8.54624334499939E-5 0.4142270497981518 >10k+ 8.54624334499939E-5 0.8281321406224337 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16962 0.38521380365360375 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13904 0.3157654006602822 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9864 0.2240153849333302 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5575 0.1266104796232072 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.271039993241385E-5 5 0.0 0.0 0.0 0.0 2.271039993241385E-5 6 0.0 0.0 0.0 0.0 4.54207998648277E-5 7 0.0 0.0 0.0 0.0 4.54207998648277E-5 8 0.0 0.0 0.0 0.0 4.54207998648277E-5 9 0.0 0.0 0.0 4.54207998648277E-5 9.08415997296554E-5 10-11 0.0 0.0 0.0 4.54207998648277E-5 9.08415997296554E-5 12-13 0.0 0.0 0.0 4.54207998648277E-5 9.08415997296554E-5 14-15 0.0 0.0 0.0 5.6775999831034626E-5 1.1355199966206925E-4 16-17 0.0 0.0 0.0 6.813119979724155E-5 1.1355199966206925E-4 18-19 0.0 0.0 0.0 6.813119979724155E-5 1.1355199966206925E-4 20-21 0.0 0.0 0.0 1.4761759956069003E-4 1.362623995944831E-4 22-23 0.0 0.0 0.0 4.201423987496562E-4 1.816831994593108E-4 24-25 0.0 0.0 0.0 0.0013285583960462102 2.271039993241385E-4 26-27 0.0 0.0 0.0 0.0031340351906731113 2.271039993241385E-4 28-29 0.0 0.0 0.0 0.007551207977527605 2.271039993241385E-4 30-31 0.0 0.0 0.0 0.024311483127649025 2.271039993241385E-4 32-33 0.0 0.0 0.0 0.0652583342057912 2.271039993241385E-4 34-35 0.0 0.0 0.0 0.12422588763030376 2.271039993241385E-4 36-37 0.0 1.1355199966206925E-5 0.0 0.21409094016286537 2.271039993241385E-4 38-39 0.0 2.271039993241385E-5 0.0 0.34766215736535744 2.3845919929034543E-4 40-41 0.0 2.271039993241385E-5 0.0 0.5031262001026964 2.4981439925655236E-4 42-43 0.0 2.271039993241385E-5 0.0 0.6487339292693678 2.611695992227593E-4 44-45 0.0 2.271039993241385E-5 0.0 0.8193117431617283 2.725247991889662E-4 46-47 0.0 2.271039993241385E-5 0.0 1.0181980705698426 3.293007990200008E-4 48-49 0.0 2.271039993241385E-5 0.0 1.2259073883516995 3.4065599898620776E-4 50-51 0.0 2.271039993241385E-5 0.0 1.4527502180766154 3.633663989186216E-4 52-53 0.0 2.271039993241385E-5 0.0 1.685406910184229 3.747215988848285E-4 54-55 0.0 2.271039993241385E-5 0.0 1.9538324821853945 3.8607679885103546E-4 56-57 0.0 2.271039993241385E-5 0.0 2.246978324512993 3.8607679885103546E-4 58-59 0.0 2.271039993241385E-5 0.0 2.5701018947513767 3.8607679885103546E-4 60-61 0.0 2.271039993241385E-5 0.0 2.8871277226079077 4.3149759871586316E-4 62-63 0.0 2.271039993241385E-5 0.0 3.215576881630443 4.54207998648277E-4 64-65 0.0 2.271039993241385E-5 0.0 3.542186498258453 4.54207998648277E-4 66-67 0.0 2.271039993241385E-5 0.0 3.9015445115890035 4.54207998648277E-4 68-69 0.0 2.271039993241385E-5 0.0 4.300316424002258 4.54207998648277E-4 70-71 0.0 2.271039993241385E-5 0.0 4.721128779549921 4.54207998648277E-4 72-73 0.0 2.271039993241385E-5 0.0 5.122660005554964 5.450495983779324E-4 74-75 1.1355199966206925E-5 2.271039993241385E-5 0.0 5.524793057158216 5.450495983779324E-4 76-77 2.271039993241385E-5 2.271039993241385E-5 0.0 5.9696897918342025 5.450495983779324E-4 78-79 2.271039993241385E-5 2.271039993241385E-5 0.0 6.458258625580222 5.450495983779324E-4 80-81 2.271039993241385E-5 3.4065599898620776E-5 0.0 6.940491257745098 5.677599983103462E-4 82-83 2.271039993241385E-5 4.54207998648277E-5 0.0 7.423938896306358 6.358911981075878E-4 84-85 2.271039993241385E-5 4.54207998648277E-5 0.0 7.918684958833994 6.358911981075878E-4 86-87 2.271039993241385E-5 4.54207998648277E-5 0.0 8.43366598770141 6.358911981075878E-4 88 2.271039993241385E-5 4.54207998648277E-5 0.0 8.831802008916558 6.358911981075878E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8135 0.0 50.4141 1 TACCTGG 5235 0.0 47.856262 2 GTATCAA 13980 0.0 40.64762 1 ACCTGGG 6020 0.0 40.604137 3 GTACCTG 6390 0.0 39.213764 1 TATCAAC 18075 0.0 31.198982 2 ATCAACG 17975 0.0 30.956001 3 CAACGCA 18065 0.0 30.822147 5 TCAACGC 18410 0.0 30.351978 4 AACGCAG 19175 0.0 29.45346 6 GTACATG 17305 0.0 29.095528 1 CCTGGGG 6175 0.0 28.719965 4 TACATGG 17595 0.0 28.130497 2 ACATGGG 18095 0.0 26.368864 3 ACGCAGA 21655 0.0 25.885405 7 CGCAGAG 22220 0.0 25.184978 8 TGGGGGG 8230 0.0 24.679323 6 CATGGGG 12930 0.0 24.31114 4 ATGGGGG 9100 0.0 23.402626 5 GTGGTAT 3935 0.0 23.372732 1 >>END_MODULE