FastQCFastQC Report
Mon 6 Jun 2016
ERR1211351_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211351_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227544
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128910.5787091074295277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111250.49942896750861043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74900.33624476104624645No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60040.26953451873453455No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31980.1435661876937111No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25310.1136228958889252No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25290.1135331109060023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43550.059.6285671
TACCTGG22250.048.83892
GTATCAA81050.043.0679661
TAACGCA2500.041.3512234
ACCTGGG26250.040.0981563
GTACCTG30400.035.7471851
TATCAAC106050.032.381442
ATCAACG105900.032.25853
CAACGCA106400.032.01935
TCAACGC109000.031.3841654
AACGCAG112000.030.7532946
GTACATG86900.029.9370381
ATAACGC3000.029.7603513
TACATGG88700.028.744822
GTGGTAT16600.028.0563091
ACGCAGA123200.027.7293177
CGCAGAG126100.027.0164728
ACATGGG90900.026.72593
CATGGGG61550.026.7205684
CCTGGGG26600.026.3214844
Produced by FastQC (version 0.11.5)