##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211351_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2227544 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38326246305348 31.0 31.0 34.0 28.0 34.0 2 31.357156132493902 31.0 31.0 34.0 28.0 34.0 3 31.406432824671477 31.0 31.0 34.0 28.0 34.0 4 35.02375396400699 37.0 35.0 37.0 32.0 37.0 5 35.08424255592706 37.0 35.0 37.0 32.0 37.0 6 35.15796994357912 37.0 35.0 37.0 32.0 37.0 7 35.09642503133496 37.0 35.0 37.0 32.0 37.0 8 35.09931431208542 37.0 35.0 37.0 32.0 37.0 9 36.66788265461872 39.0 37.0 39.0 32.0 39.0 10-11 36.58729277625942 39.0 37.0 39.0 32.0 39.0 12-13 36.49893425225271 39.0 37.0 39.0 32.0 39.0 14-15 37.61445879407994 40.0 37.0 41.0 32.0 41.0 16-17 37.60815184795452 40.0 37.0 41.0 32.0 41.0 18-19 37.63272263084366 40.0 37.0 41.0 32.0 41.0 20-21 37.59309513078081 40.0 37.0 41.0 32.0 41.0 22-23 37.45474275704542 40.0 37.0 41.0 31.5 41.0 24-25 37.33626765621689 40.0 37.0 41.0 31.0 41.0 26-27 37.18543763894226 40.0 36.0 41.0 31.0 41.0 28-29 37.02128981515068 39.5 36.0 41.0 30.5 41.0 30-31 36.79249770150444 39.0 36.0 41.0 30.0 41.0 32-33 36.59684948984173 39.0 35.0 41.0 30.0 41.0 34-35 36.402528524689075 39.0 35.0 41.0 29.5 41.0 36-37 36.261997742805534 39.0 35.0 41.0 29.0 41.0 38-39 36.08733160826453 39.0 35.0 40.5 28.0 41.0 40-41 35.90252358651502 39.0 35.0 40.0 27.0 41.0 42-43 35.73026526075355 38.0 35.0 40.0 27.0 41.0 44-45 35.568519185255155 38.0 35.0 40.0 26.0 41.0 46-47 35.35515549861192 38.0 34.0 40.0 24.5 41.0 48-49 35.0876902992713 38.0 34.0 40.0 24.0 41.0 50-51 34.40177141282058 37.5 33.5 39.5 23.5 40.5 52-53 34.63981923589388 38.0 33.5 40.0 24.0 40.5 54-55 34.99751856753447 38.0 34.0 40.0 24.0 41.0 56-57 34.90126996369095 38.0 34.0 40.0 24.0 41.0 58-59 34.69664347819841 38.0 34.0 40.0 23.0 41.0 60-61 34.42903305164792 37.5 33.0 40.0 23.0 41.0 62-63 34.143775611166376 37.0 33.0 40.0 22.5 41.0 64-65 33.86395263123871 36.0 33.0 40.0 22.0 41.0 66-67 33.639317337839344 36.0 33.0 39.0 22.0 41.0 68-69 33.29899454286874 35.5 32.0 39.0 21.5 41.0 70-71 32.944054752678284 35.0 32.0 39.0 20.0 40.5 72-73 32.57165784379568 35.0 31.5 37.5 20.0 40.0 74-75 32.20182676526255 35.0 31.0 37.0 19.5 39.5 76-77 31.83880183735989 35.0 31.0 36.5 19.0 39.0 78-79 31.464818203366576 35.0 30.5 36.0 18.0 39.0 80-81 31.123647164769807 35.0 30.0 36.0 18.0 37.5 82-83 30.80571988701458 34.0 30.0 35.0 17.0 37.0 84-85 30.49388968298718 34.0 29.0 35.0 15.5 37.0 86-87 30.221110110507357 34.0 29.0 35.0 15.0 36.0 88-89 29.949279565297026 34.0 29.0 35.0 12.5 36.0 90-91 29.67019574024127 34.0 29.0 35.0 11.0 35.5 92-93 29.41239836339933 34.0 28.5 35.0 10.0 35.0 94-95 29.16406813961924 34.0 27.0 35.0 10.0 35.0 96-97 28.933155080213904 34.0 27.0 35.0 10.0 35.0 98-99 28.673745614003586 33.5 25.5 35.0 10.0 35.0 100 27.032468045524578 31.0 23.0 34.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3547591950765323 1101 2 -0.3151472356731091 1101 3 -0.35654163547116013 1101 4 -0.27329556618833095 1101 5 -0.4001232195719595 1101 6 -0.38591296802217556 1101 7 -0.5631113433696342 1101 8 -0.48499718676898596 1101 9 -0.5824315988068491 1101 10-11 -0.5163143071550067 1101 12-13 -0.5342396945622383 1101 14-15 -0.6452213231248507 1101 16-17 -0.6611924689927378 1101 18-19 -0.6475449063464538 1101 20-21 -0.6022511010934721 1101 22-23 -0.6196866985238572 1101 24-25 -0.6258435475957214 1101 26-27 -0.6336439915872702 1101 28-29 -0.7954740230884667 1101 30-31 -0.7008346583350331 1101 32-33 -0.7788253497472866 1101 34-35 -0.7586817018658323 1101 36-37 -0.7762397411676645 1101 38-39 -0.7996761901922937 1101 40-41 -0.8914787503158621 1101 42-43 -0.8425239455756284 1101 44-45 -0.8765048573888095 1101 46-47 -0.9391537201193572 1101 48-49 -0.7783969986305053 1101 50-51 -0.5615340803442734 1101 52-53 -0.4266002563638125 1101 54-55 -0.5472629303961654 1101 56-57 -0.387876354195825 1101 58-59 -0.5892860568021163 1101 60-61 -0.5712311670315842 1101 62-63 -0.7062373515097207 1101 64-65 -0.616587998686903 1101 66-67 -0.5061515478407799 1101 68-69 -0.7400125158918343 1101 70-71 -0.5860982837072655 1101 72-73 -0.6091077927841582 1101 74-75 -0.8449526351570107 1101 76-77 -0.6104655313763097 1101 78-79 -0.7965909568097054 1101 80-81 -0.896907332027677 1101 82-83 -0.9220121641520755 1101 84-85 -0.8555282499403631 1101 86-87 -0.8395426100179719 1101 88-89 -0.9698808514650104 1101 90-91 -0.88793187675574 1101 92-93 -0.921985092504741 1101 94-95 -1.0357136551438586 1101 96-97 -1.1629467025449998 1101 98-99 -1.2831486829542165 1101 100 -1.3704998090828546 1102 1 -0.2650513672431316 1102 2 -0.29977668854037987 1102 3 -0.33654537447548094 1102 4 -0.20284086537867552 1102 5 -0.20283089559409717 1102 6 -0.16166494850028812 1102 7 -0.28619689491806355 1102 8 -0.29630731796082443 1102 9 -0.22240666113044938 1102 10-11 -0.28838342328129585 1102 12-13 -0.3930915951266911 1102 14-15 -0.5375115071953118 1102 16-17 -0.512048610389698 1102 18-19 -0.5867044155101695 1102 20-21 -0.5284697296255914 1102 22-23 -0.5937545622758691 1102 24-25 -0.6142185933381299 1102 26-27 -0.5210560378246498 1102 28-29 -0.5128890259393941 1102 30-31 -0.47860959937433734 1102 32-33 -0.5583476301775576 1102 34-35 -0.5065741195658688 1102 36-37 -0.5304875053830855 1102 38-39 -0.6781486669202863 1102 40-41 -0.6306853950920583 1102 42-43 -0.7088603060749392 1102 44-45 -0.6327834060731377 1102 46-47 -0.7168095181428455 1102 48-49 -0.5702851711056667 1102 50-51 -0.4746228191056403 1102 52-53 -0.5191272989459179 1102 54-55 -0.6539351863526974 1102 56-57 -0.6596558484749053 1102 58-59 -0.6807517931649798 1102 60-61 -0.6385874631222919 1102 62-63 -0.5434717950418388 1102 64-65 -0.59851765434437 1102 66-67 -0.5112565789977737 1102 68-69 -0.4574501668966846 1102 70-71 -0.6314868313182203 1102 72-73 -0.6354046329411744 1102 74-75 -0.5757989541182376 1102 76-77 -0.5374632564035586 1102 78-79 -0.707599085163519 1102 80-81 -0.6216153149666148 1102 82-83 -0.6335589616893813 1102 84-85 -0.8875741523951817 1102 86-87 -0.798788011962305 1102 88-89 -0.8152647870882532 1102 90-91 -0.8154706867695722 1102 92-93 -0.8193951319784141 1102 94-95 -0.9766991545603574 1102 96-97 -1.0594998182309467 1102 98-99 -1.0530891471343118 1102 100 -1.3488037011886966 1103 1 -0.45655803958888086 1103 2 -0.42495208954141006 1103 3 -0.34970380412602964 1103 4 -0.3160091528245488 1103 5 -0.2901645851543222 1103 6 -0.3557104681323153 1103 7 -0.3463597371941347 1103 8 -0.42910058936453055 1103 9 -0.5574659500565815 1103 10-11 -0.455971789382275 1103 12-13 -0.6093882313333836 1103 14-15 -0.5995167740745373 1103 16-17 -0.6487038258941169 1103 18-19 -0.6513198724800944 1103 20-21 -0.7104878089816395 1103 22-23 -0.674330746426925 1103 24-25 -0.7616751360047118 1103 26-27 -0.5696298592933289 1103 28-29 -0.7107963568177524 1103 30-31 -0.6741436195294099 1103 32-33 -0.6681179010331064 1103 34-35 -0.8043887446547799 1103 36-37 -0.7914507156126618 1103 38-39 -0.8094046802418617 1103 40-41 -0.8015787797142693 1103 42-43 -0.8110658482815793 1103 44-45 -0.5964050856767287 1103 46-47 -0.7715875065095901 1103 48-49 -0.8203583074442946 1103 50-51 -0.9154816270457644 1103 52-53 -0.6698277471168979 1103 54-55 -0.7313389406581692 1103 56-57 -0.6166003995718938 1103 58-59 -0.7386486710603677 1103 60-61 -0.6760615664814082 1103 62-63 -0.7352749983736118 1103 64-65 -0.7146422175523739 1103 66-67 -0.7294094566636034 1103 68-69 -0.6560322651935238 1103 70-71 -0.6290595817148485 1103 72-73 -0.7753406874688693 1103 74-75 -0.8161722589959339 1103 76-77 -0.657141807249193 1103 78-79 -0.7602652202355102 1103 80-81 -0.625883660629281 1103 82-83 -0.6719985178600822 1103 84-85 -0.743270111416507 1103 86-87 -0.6580517754772508 1103 88-89 -0.9302802818951115 1103 90-91 -0.7831800300730087 1103 92-93 -0.9715593697569389 1103 94-95 -1.173769104253715 1103 96-97 -1.3093196012584798 1103 98-99 -1.3020059189109396 1103 100 -1.1055741599003461 1104 1 -0.39858515719104304 1104 2 -0.2144353983589582 1104 3 -0.4187592855674289 1104 4 -0.26769079801245965 1104 5 -0.24461765496555188 1104 6 -0.24636136548758003 1104 7 -0.28221326753193665 1104 8 -0.2759766669075958 1104 9 -0.4158128327204551 1104 10-11 -0.3787315652404075 1104 12-13 -0.42131400984412437 1104 14-15 -0.5765804587261911 1104 16-17 -0.4945548895865244 1104 18-19 -0.5094736852552515 1104 20-21 -0.6182811170114135 1104 22-23 -0.5425440815510498 1104 24-25 -0.6037902004926394 1104 26-27 -0.6192112398657201 1104 28-29 -0.5046067557298457 1104 30-31 -0.7533043402031296 1104 32-33 -0.7087461336623093 1104 34-35 -0.6663834213498916 1104 36-37 -0.6786582534743815 1104 38-39 -0.6476277398894794 1104 40-41 -0.7161737010076195 1104 42-43 -0.7369540020556187 1104 44-45 -0.832778032129859 1104 46-47 -0.877810555825711 1104 48-49 -0.8482040220030527 1104 50-51 -0.9228991267971622 1104 52-53 -0.8585802315195679 1104 54-55 -0.7929173870848345 1104 56-57 -0.7425830865623411 1104 58-59 -0.8368688120595067 1104 60-61 -0.6948828457534475 1104 62-63 -0.7501819309386306 1104 64-65 -0.7958785363875975 1104 66-67 -0.8239143906001232 1104 68-69 -0.7066639717892755 1104 70-71 -0.7365151788690412 1104 72-73 -0.7242346359224356 1104 74-75 -0.7993232628947808 1104 76-77 -0.8270847644600003 1104 78-79 -0.790278354153692 1104 80-81 -0.7653237708663774 1104 82-83 -0.9722206450899087 1104 84-85 -0.9070383190693079 1104 86-87 -1.0274567198744151 1104 88-89 -1.0852477312158761 1104 90-91 -0.9835998763661316 1104 92-93 -0.9797599971372186 1104 94-95 -0.9844753784439533 1104 96-97 -0.9933454248403848 1104 98-99 -1.2324223687237428 1104 100 -1.356392220910429 1105 1 -0.5064807686067283 1105 2 -0.5392755684175228 1105 3 -0.5051181662531761 1105 4 -0.4155299858277175 1105 5 -0.3754374465527235 1105 6 -0.305296456191229 1105 7 -0.37511231310654836 1105 8 -0.3042421335470138 1105 9 -0.6493299776665822 1105 10-11 -0.6480902514469022 1105 12-13 -0.5799403955087925 1105 14-15 -0.6942961645258592 1105 16-17 -0.7159711170337033 1105 18-19 -0.7764055658203759 1105 20-21 -0.6864651731592986 1105 22-23 -0.7214108337229348 1105 24-25 -0.6661992075898979 1105 26-27 -0.6174475099998631 1105 28-29 -0.6639461909522524 1105 30-31 -0.6446629377861655 1105 32-33 -0.6089423361472086 1105 34-35 -0.5876818534532404 1105 36-37 -0.6332395938056834 1105 38-39 -0.6837545994434606 1105 40-41 -0.7725872082070424 1105 42-43 -0.8247664305106355 1105 44-45 -0.9271369029685559 1105 46-47 -0.7591267618555477 1105 48-49 -0.9148989579110633 1105 50-51 -0.7994151417942206 1105 52-53 -0.6557348031359282 1105 54-55 -0.6650563880522995 1105 56-57 -0.608783454349684 1105 58-59 -0.4799041161979716 1105 60-61 -0.492585159564193 1105 62-63 -0.5781146903530257 1105 64-65 -0.5324264804465031 1105 66-67 -0.6005129977347963 1105 68-69 -0.550515965693954 1105 70-71 -0.5944927669816806 1105 72-73 -0.5416327743375042 1105 74-75 -0.5887449456080418 1105 76-77 -0.57242144743174 1105 78-79 -0.4756760396044264 1105 80-81 -0.4694367608494616 1105 82-83 -0.6116366657490211 1105 84-85 -0.5930456600455933 1105 86-87 -0.6582579251534177 1105 88-89 -0.7103149988808717 1105 90-91 -0.902717063654574 1105 92-93 -0.9071201505542668 1105 94-95 -0.8411490939577355 1105 96-97 -0.8340842736709249 1105 98-99 -0.8247879503274831 1105 100 -0.9108326253288013 1106 1 -0.5524729602060283 1106 2 -0.5844510499207445 1106 3 -0.5385518961331073 1106 4 -0.34217825304854443 1106 5 -0.40744032801266883 1106 6 -0.46551343526309097 1106 7 -0.5778066164918698 1106 8 -0.5255152329050148 1106 9 -0.49670944005097795 1106 10-11 -0.5906567073401092 1106 12-13 -0.5761560089504627 1106 14-15 -0.6875260676767851 1106 16-17 -0.5972260145768473 1106 18-19 -0.7234703502494284 1106 20-21 -0.6408635498182846 1106 22-23 -0.5826034676204088 1106 24-25 -0.5834464153109451 1106 26-27 -0.6877920401173441 1106 28-29 -0.6401887217357469 1106 30-31 -0.7736759587044233 1106 32-33 -0.7071115814043125 1106 34-35 -0.6545297971521293 1106 36-37 -0.6655648608298605 1106 38-39 -0.5890369795530859 1106 40-41 -0.6101854184607376 1106 42-43 -0.6343667514633822 1106 44-45 -0.7230564651763771 1106 46-47 -0.7262345409080453 1106 48-49 -0.712464292248363 1106 50-51 -0.733322642582273 1106 52-53 -0.5570228137526669 1106 54-55 -0.7406228231942578 1106 56-57 -0.48875893822447125 1106 58-59 -0.49472841730065653 1106 60-61 -0.691772253864805 1106 62-63 -0.5330661867655309 1106 64-65 -0.6664580935008217 1106 66-67 -0.8097520986323445 1106 68-69 -0.7028027539629775 1106 70-71 -0.6916765746788158 1106 72-73 -0.7882905871354922 1106 74-75 -0.8604062910601051 1106 76-77 -0.8309966564018154 1106 78-79 -0.8514869442899631 1106 80-81 -1.0821350959071268 1106 82-83 -0.9357747228584508 1106 84-85 -0.7883611631103591 1106 86-87 -0.7665285847567525 1106 88-89 -0.9463778139170316 1106 90-91 -0.843854894476511 1106 92-93 -0.887695987990913 1106 94-95 -0.965102514307997 1106 96-97 -0.9080372987591936 1106 98-99 -0.8961473156157744 1106 100 -0.9374238760069247 1107 1 -0.48401954258793367 1107 2 -0.5271381284073442 1107 3 -0.3774972854364904 1107 4 -0.21352549527883724 1107 5 -0.28902453752050405 1107 6 -0.38233646354196793 1107 7 -0.4213475321091593 1107 8 -0.2634280860923326 1107 9 -0.5201546035916067 1107 10-11 -0.42851819651424705 1107 12-13 -0.49726434879389103 1107 14-15 -0.5239899778877088 1107 16-17 -0.6827020634180485 1107 18-19 -0.5732390927389304 1107 20-21 -0.6419242764159989 1107 22-23 -0.6652867475590583 1107 24-25 -0.6442415780154249 1107 26-27 -0.6006841685225979 1107 28-29 -0.6251080085643466 1107 30-31 -0.4907251080924766 1107 32-33 -0.5534392933644838 1107 34-35 -0.6557471242644439 1107 36-37 -0.6533908729995517 1107 38-39 -0.7483294462441137 1107 40-41 -0.7254006336759531 1107 42-43 -0.8208093993618704 1107 44-45 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2208 54-55 1.0389927487941932 2208 56-57 1.0464445461315464 2208 58-59 1.1144370435597182 2208 60-61 1.070614830556174 2208 62-63 1.219459341183729 2208 64-65 1.1554758258907256 2208 66-67 1.1333592936315924 2208 68-69 1.1198055686453543 2208 70-71 1.047186986218641 2208 72-73 1.1180824929664013 2208 74-75 1.0994003678990936 2208 76-77 1.008205742122616 2208 78-79 1.070232596073641 2208 80-81 1.0323419719597702 2208 82-83 1.079066995373097 2208 84-85 1.1089411028359777 2208 86-87 1.2233977822882594 2208 88-89 1.2468420403197733 2208 90-91 1.1102226697705468 2208 92-93 1.1551725464030405 2208 94-95 1.1487886165322863 2208 96-97 1.2276977098571713 2208 98-99 1.2328097285955089 2208 100 1.5867237583102032 2209 1 0.39508672671747647 2209 2 0.29465569101502354 2209 3 0.40795325713148856 2209 4 0.3001153327838111 2209 5 0.2787022758237754 2209 6 0.3691783141403846 2209 7 0.4514823145863218 2209 8 0.3916068593661848 2209 9 0.569988072570986 2209 10-11 0.46154002219260093 2209 12-13 0.4989018520914925 2209 14-15 0.6305116519513945 2209 16-17 0.7024813468601394 2209 18-19 0.6894653488691773 2209 20-21 0.6778022332610192 2209 22-23 0.7046421278756867 2209 24-25 0.745291865713611 2209 26-27 0.6824907675412106 2209 28-29 0.7344055544489834 2209 30-31 0.8561402392032278 2209 32-33 0.7264100117594339 2209 34-35 0.7850164090938776 2209 36-37 0.8411426419014987 2209 38-39 0.8713263658997192 2209 40-41 0.8370248136401557 2209 42-43 0.819174563211142 2209 44-45 0.8230253024759762 2209 46-47 0.7766383120642146 2209 48-49 0.8577562264811007 2209 50-51 0.9595691557767196 2209 52-53 0.761046149843132 2209 54-55 0.8095753869443101 2209 56-57 0.7589374659331583 2209 58-59 0.6739679132246934 2209 60-61 0.7516305923213409 2209 62-63 0.753845277382517 2209 64-65 0.7941900297715563 2209 66-67 0.7475089397420192 2209 68-69 0.6706149977848028 2209 70-71 0.7959959885404686 2209 72-73 0.7950913037016605 2209 74-75 0.90328304439862 2209 76-77 0.8216309214742701 2209 78-79 0.8841226215382854 2209 80-81 0.8402373759683286 2209 82-83 0.8329389212031053 2209 84-85 0.8080625966334836 2209 86-87 0.9038383030057631 2209 88-89 0.7479719035937187 2209 90-91 0.8206692718369197 2209 92-93 0.778866299943143 2209 94-95 0.812737850908114 2209 96-97 0.8815324897171557 2209 98-99 1.0484038956352428 2209 100 1.2711928918543762 2210 1 0.6462697385366702 2210 2 0.6195204471542013 2210 3 0.7401284515127564 2210 4 0.6100574233250668 2210 5 0.5668742669915829 2210 6 0.5438697637752767 2210 7 0.7229052359441184 2210 8 0.6379781202435097 2210 9 0.7501765137315246 2210 10-11 0.7627104908546798 2210 12-13 0.7369339851740122 2210 14-15 0.9542403671931012 2210 16-17 0.9999938843671856 2210 18-19 0.9750401155024235 2210 20-21 0.9291335029373968 2210 22-23 0.9965132580480471 2210 24-25 1.031725217102064 2210 26-27 1.0726089444215745 2210 28-29 1.0806806887888314 2210 30-31 1.1267032951231073 2210 32-33 1.0528226612885518 2210 34-35 1.1308143761230767 2210 36-37 1.0674404066581644 2210 38-39 1.1476523433431325 2210 40-41 1.1048759862558413 2210 42-43 1.1565904619076335 2210 44-45 1.161222944965111 2210 46-47 1.2032705610108465 2210 48-49 1.111095531311463 2210 50-51 1.1241912752764733 2210 52-53 0.9660053299651281 2210 54-55 0.9614702323470752 2210 56-57 0.9733985584157807 2210 58-59 0.9238800357905532 2210 60-61 0.985283543880648 2210 62-63 1.1230405641688108 2210 64-65 1.0139240696399625 2210 66-67 1.1303524621618806 2210 68-69 1.0985854094941558 2210 70-71 1.0139587688875622 2210 72-73 1.1036884513952643 2210 74-75 1.1112665944058904 2210 76-77 1.0988784631284823 2210 78-79 1.0578177753595845 2210 80-81 1.2067729861044079 2210 82-83 1.1605019251423343 2210 84-85 1.2321837281241912 2210 86-87 1.0820263931085705 2210 88-89 1.0889812473041012 2210 90-91 1.3163816967945614 2210 92-93 1.319085537756127 2210 94-95 1.3628029773905972 2210 96-97 1.4181722946917823 2210 98-99 1.5897304719861545 2210 100 1.539693445937985 2211 1 0.4074435515706867 2211 2 0.4479404170817709 2211 3 0.4415905913374516 2211 4 0.32160503418457864 2211 5 0.28588772382722283 2211 6 0.4459126469634427 2211 7 0.35608894761789855 2211 8 0.34893362406921113 2211 9 0.4127270539401948 2211 10-11 0.5270901263030012 2211 12-13 0.5825271951104156 2211 14-15 0.6909386428059676 2211 16-17 0.6151681721690849 2211 18-19 0.6552259898336175 2211 20-21 0.6386053215333405 2211 22-23 0.64228743229868 2211 24-25 0.6053494831805466 2211 26-27 0.6390714637472854 2211 28-29 0.6433857208014828 2211 30-31 0.5984343139131738 2211 32-33 0.5712834991328251 2211 34-35 0.5067038716914638 2211 36-37 0.4894926769785215 2211 38-39 0.6699914964990654 2211 40-41 0.6900071077746333 2211 42-43 0.7201041230187712 2211 44-45 0.6282601840545965 2211 46-47 0.6047221203755626 2211 48-49 0.7546877526939895 2211 50-51 0.7712210781395754 2211 52-53 0.6096009689928792 2211 54-55 0.6243072139454782 2211 56-57 0.516327884787934 2211 58-59 0.5569783063339102 2211 60-61 0.6388791416118451 2211 62-63 0.5774070260188466 2211 64-65 0.6360309795632233 2211 66-67 0.6229720573161615 2211 68-69 0.5388746618970757 2211 70-71 0.6674993994915255 2211 72-73 0.6542142062712131 2211 74-75 0.6996723896499191 2211 76-77 0.6130166917828994 2211 78-79 0.663088510533516 2211 80-81 0.6165037910068456 2211 82-83 0.6256892213563354 2211 84-85 0.7181174124212077 2211 86-87 0.7351526342299906 2211 88-89 0.818406417890877 2211 90-91 0.8664258484987108 2211 92-93 0.9480763683564106 2211 94-95 1.0768720984106963 2211 96-97 1.1452524622532287 2211 98-99 1.0780582879584877 2211 100 1.1997476811195398 2212 1 0.5674137953895979 2212 2 0.4523237840489358 2212 3 0.531678683195274 2212 4 0.47003350381825726 2212 5 0.3567237602605928 2212 6 0.4774052225911234 2212 7 0.508643366470622 2212 8 0.569626535094983 2212 9 0.7283271962820947 2212 10-11 0.7343916161919211 2212 12-13 0.7650523335846344 2212 14-15 0.7999593328108929 2212 16-17 0.7581615944119733 2212 18-19 0.7373777229944096 2212 20-21 0.7708540813838098 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1.017129286815237 2212 90-91 1.0445475337287995 2212 92-93 1.0983304099005302 2212 94-95 1.0898493291199642 2212 96-97 1.1756631568138936 2212 98-99 1.222657615167929 2212 100 1.2335833708153352 2213 1 0.3595553385236059 2213 2 0.4309744796206836 2213 3 0.46122648551748924 2213 4 0.2561585681708962 2213 5 0.23997343047093267 2213 6 0.3992461980700952 2213 7 0.35693533199450656 2213 8 0.4661759382066606 2213 9 0.5283490508321762 2213 10-11 0.5689419700891776 2213 12-13 0.5412286491102662 2213 14-15 0.7221261551255154 2213 16-17 0.6513831071749223 2213 18-19 0.6528000509095335 2213 20-21 0.6721420635216546 2213 22-23 0.715976129807629 2213 24-25 0.6565893113518229 2213 26-27 0.6732844896070347 2213 28-29 0.8507228808028984 2213 30-31 0.8426738606543083 2213 32-33 0.8336477886641234 2213 34-35 0.8207428380387896 2213 36-37 0.8663783007566863 2213 38-39 0.9620782143927187 2213 40-41 0.8380466174384722 2213 42-43 0.9664567689366734 2213 44-45 1.009868138476314 2213 46-47 0.9702217593744038 2213 48-49 1.0098737590272862 2213 50-51 1.0998608380752088 2213 52-53 1.0060688812112417 2213 54-55 0.9594505785879122 2213 56-57 1.0769284058804018 2213 58-59 1.0291202828110428 2213 60-61 0.9814767752534621 2213 62-63 0.9229386665275925 2213 64-65 0.9024676301425885 2213 66-67 0.8864162093487451 2213 68-69 0.901221403924481 2213 70-71 0.8337918729664509 2213 72-73 0.9466878905992999 2213 74-75 0.8709777675899275 2213 76-77 0.7158235664956756 2213 78-79 0.9447833230055629 2213 80-81 0.9102473310964001 2213 82-83 0.9038873760904202 2213 84-85 0.831825516883157 2213 86-87 0.9031184024979595 2213 88-89 0.9258720362302917 2213 90-91 0.9806542357532742 2213 92-93 0.8990102948149215 2213 94-95 0.9897499979367907 2213 96-97 0.9940165009543414 2213 98-99 1.1034468178936656 2213 100 0.90762585425826 2214 1 0.49109243130407165 2214 2 0.42131298985566445 2214 3 0.495659106430022 2214 4 0.3118184136800295 2214 5 0.3491492917999466 2214 6 0.4600867738947727 2214 7 0.3513189441360538 2214 8 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0.6794415138268732 2214 74-75 0.7857806379569467 2214 76-77 0.7513372234948115 2214 78-79 0.7664853386513961 2214 80-81 0.6794788279476123 2214 82-83 0.7821159579278252 2214 84-85 0.6797456304784291 2214 86-87 0.7207476146875074 2214 88-89 0.8241583643128543 2214 90-91 0.8536088479126569 2214 92-93 1.013782882658564 2214 94-95 0.9356256966239229 2214 96-97 0.9715741242316405 2214 98-99 0.9639398541294533 2214 100 1.0708035219985774 2215 1 0.2678780747475926 2215 2 -0.00588560832387941 2215 3 0.1910608959565785 2215 4 0.1938964538209902 2215 5 0.22228440596345678 2215 6 0.14978781793094953 2215 7 0.18157642644015226 2215 8 0.13541168292024253 2215 9 0.3040179046689744 2215 10-11 0.3276061026047685 2215 12-13 0.3991781977146047 2215 14-15 0.45186045377997885 2215 16-17 0.3922069488856863 2215 18-19 0.42621343704989556 2215 20-21 0.41553406776233004 2215 22-23 0.5415096473429841 2215 24-25 0.4356965724087445 2215 26-27 0.4384389968345843 2215 28-29 0.37257505868052476 2215 30-31 0.39059561416234345 2215 32-33 0.4150677143424488 2215 34-35 0.4562213421572423 2215 36-37 0.6697484561840099 2215 38-39 0.737239574265061 2215 40-41 0.7496455770449373 2215 42-43 0.7813703349761099 2215 44-45 0.7382500354092443 2215 46-47 0.7388667896276644 2215 48-49 0.7723882127520199 2215 50-51 0.6072740539293946 2215 52-53 0.5219476539423837 2215 54-55 0.6444379307204713 2215 56-57 0.664786659128886 2215 58-59 0.5869201443848766 2215 60-61 0.7073366517135966 2215 62-63 0.7027845438171809 2215 64-65 0.7506262736759695 2215 66-67 0.7666120659018816 2215 68-69 0.6656201976392779 2215 70-71 0.7138533545110022 2215 72-73 0.6394914462842465 2215 74-75 0.7150534570428988 2215 76-77 0.8398638529060172 2215 78-79 0.6899346991649544 2215 80-81 0.6954535833451381 2215 82-83 0.8601655797361936 2215 84-85 0.6466785901364211 2215 86-87 0.6420658692166299 2215 88-89 0.7751184512945741 2215 90-91 0.8583419419162581 2215 92-93 0.9531830611136414 2215 94-95 0.9189633445268086 2215 96-97 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0.49113975089527884 2311 6 0.33244260039786155 2311 7 0.39185273841338386 2311 8 0.49551750943920325 2311 9 0.6423839859276939 2311 10-11 0.6644280026626319 2311 12-13 0.7039783408560112 2311 14-15 0.8552983726886438 2311 16-17 0.8114982834917157 2311 18-19 0.6782363916212546 2311 20-21 0.7637682482561345 2311 22-23 0.8790590145338726 2311 24-25 0.8984505870778605 2311 26-27 0.936778080354415 2311 28-29 0.8863975835390789 2311 30-31 0.9073563616721572 2311 32-33 1.0514819066973757 2311 34-35 1.0655931360855604 2311 36-37 1.0323959805735967 2311 38-39 1.1521192820949864 2311 40-41 1.1929444092592618 2311 42-43 1.2961164249214434 2311 44-45 1.3111053312805083 2311 46-47 1.2114988599401144 2311 48-49 1.1343796242676873 2311 50-51 1.0920437073742377 2311 52-53 1.011525242500305 2311 54-55 0.9659268177816145 2311 56-57 0.9847872074528183 2311 58-59 0.8765105031185882 2311 60-61 0.9920116471322089 2311 62-63 1.044979657673764 2311 64-65 0.9691376896359429 2311 66-67 0.8767370837584849 2311 68-69 0.7995479574041298 2311 70-71 0.7921591345667309 2311 72-73 0.7847690064159067 2311 74-75 0.8890310854264527 2311 76-77 0.9083334230921984 2311 78-79 0.9879742968106235 2311 80-81 1.057464691233644 2311 82-83 1.044665905123157 2311 84-85 1.1117725771873168 2311 86-87 1.0566980376502002 2311 88-89 1.1836333447826526 2311 90-91 1.1434362608474338 2311 92-93 1.1523774578202328 2311 94-95 1.1377901910437238 2311 96-97 1.3725395309979653 2311 98-99 1.2095426835168475 2311 100 1.2754798942738965 2312 1 0.42857195966560013 2312 2 0.49983296392714394 2312 3 0.5835312058805222 2312 4 0.48370127371867255 2312 5 0.3981757859785944 2312 6 0.4222927421995877 2312 7 0.380684101165194 2312 8 0.441933690352478 2312 9 0.6403162386574479 2312 10-11 0.6107038581655715 2312 12-13 0.6357984194261945 2312 14-15 0.8291219968292509 2312 16-17 0.8240881015462236 2312 18-19 0.7846646662800367 2312 20-21 0.861409274916916 2312 22-23 0.8839129329947966 2312 24-25 0.8352254406438107 2312 26-27 0.8348782565784276 2312 28-29 0.8834211482654766 2312 30-31 0.8292705810425787 2312 32-33 0.9831941237605193 2312 34-35 0.9046010241361628 2312 36-37 0.9651300950291244 2312 38-39 0.9623020862591574 2312 40-41 0.9439934226729534 2312 42-43 1.033182986618975 2312 44-45 0.9993561249513832 2312 46-47 1.034967610683914 2312 48-49 1.0191733662515077 2312 50-51 1.0992128067999474 2312 52-53 0.8965133159702674 2312 54-55 0.8898614820295379 2312 56-57 0.8644676963607054 2312 58-59 0.832560855762452 2312 60-61 0.8712268703076163 2312 62-63 0.8075898880665378 2312 64-65 0.8172560335942407 2312 66-67 0.7490796006856613 2312 68-69 0.7615048573921115 2312 70-71 0.7692579221491584 2312 72-73 0.8883529610827381 2312 74-75 0.8615754359389882 2312 76-77 0.887870995503274 2312 78-79 0.9551718951747468 2312 80-81 0.9095711993499229 2312 82-83 0.75731531816675 2312 84-85 0.8711202982815607 2312 86-87 1.0463169701767967 2312 88-89 1.0530172778169238 2312 90-91 0.9461659252625658 2312 92-93 1.0211707135234143 2312 94-95 1.03764560239226 2312 96-97 1.158435870666505 2312 98-99 1.165093875401567 2312 100 1.220557146312192 2313 1 0.5332853544996539 2313 2 0.38995681659238457 2313 3 0.47497817529170305 2313 4 0.42360821010634453 2313 5 0.38789598574469863 2313 6 0.3818406857752379 2313 7 0.4742883356030845 2313 8 0.4712642533481599 2313 9 0.5420594039810211 2313 10-11 0.5342552346770972 2313 12-13 0.6193774944777743 2313 14-15 0.7335113230974528 2313 16-17 0.7263191471262118 2313 18-19 0.7201608749607544 2313 20-21 0.6593453056537371 2313 22-23 0.66072218886314 2313 24-25 0.6949633854672754 2313 26-27 0.7057086033833002 2313 28-29 0.7306383083018062 2313 30-31 0.8162248443542737 2313 32-33 0.7359841459019449 2313 34-35 0.8495016779319755 2313 36-37 0.8514066219310905 2313 38-39 0.8597223060483117 2313 40-41 0.7573061332413502 2313 42-43 0.678518125895188 2313 44-45 0.8980480980334491 2313 46-47 0.8789287050446717 2313 48-49 0.8664454445561134 2313 50-51 0.7436234764691037 2313 52-53 0.6594822251207972 2313 54-55 0.660419196268812 2313 56-57 0.6324370698136406 2313 58-59 0.616825429779503 2313 60-61 0.6469646032931067 2313 62-63 0.6079167134279047 2313 64-65 0.6698443743805953 2313 66-67 0.6322590854841081 2313 68-69 0.5748807566152792 2313 70-71 0.6436495409104595 2313 72-73 0.7638632584394074 2313 74-75 0.7242400093756629 2313 76-77 0.7028268287374537 2313 78-79 0.7655524635389135 2313 80-81 0.6787854110660128 2313 82-83 0.5908613116723167 2313 84-85 0.6785090906020024 2313 86-87 0.6627397813537179 2313 88-89 0.8122725657859782 2313 90-91 0.7848286763123227 2313 92-93 0.8370902644269158 2313 94-95 0.8976098220885476 2313 96-97 1.020811851142593 2313 98-99 0.9149568336462721 2313 100 0.9858911683607516 2314 1 0.27083626451164733 2314 2 0.20087653118148552 2314 3 0.24614829701778973 2314 4 0.161409842088851 2314 5 0.1521333301525445 2314 6 0.17538802958203803 2314 7 0.22131645023682722 2314 8 0.27226141698431405 2314 9 0.3635522300698426 2314 10-11 0.3950050107159768 2314 12-13 0.4404415745485295 2314 14-15 0.49956947857369727 2314 16-17 0.5533083225772089 2314 18-19 0.591926032048363 2314 20-21 0.5111687641858609 2314 22-23 0.4488117504145279 2314 24-25 0.5873370442049008 2314 26-27 0.6497920228188292 2314 28-29 0.5791009905117122 2314 30-31 0.6098448860840335 2314 32-33 0.7345977335444402 2314 34-35 0.7035571630475133 2314 36-37 0.5016727993655437 2314 38-39 0.6084210418486009 2314 40-41 0.7484478627077564 2314 42-43 0.6041781314477035 2314 44-45 0.7122415088539995 2314 46-47 0.4660633908265055 2314 48-49 0.5005444911829642 2314 50-51 0.6541951569073561 2314 52-53 0.5651627520948637 2314 54-55 0.5826123838437667 2314 56-57 0.5745367518317792 2314 58-59 0.5315020385072629 2314 60-61 0.3808849103416563 2314 62-63 0.6262445324043497 2314 64-65 0.6436759842388895 2314 66-67 0.6305544050544398 2314 68-69 0.6907175268812509 2314 70-71 0.5672020222279528 2314 72-73 0.6009824887573032 2314 74-75 0.599899084738226 2314 76-77 0.5606485098912337 2314 78-79 0.6375231926074925 2314 80-81 0.7161420544826989 2314 82-83 0.6634371650858313 2314 84-85 0.6381110837844233 2314 86-87 0.6384573871306287 2314 88-89 0.5558462411239269 2314 90-91 0.5087371341718558 2314 92-93 0.7481969471977941 2314 94-95 0.8541921324514377 2314 96-97 0.8828204604084284 2314 98-99 0.926023446695833 2314 100 0.9522474854954233 2315 1 0.4241799639045212 2315 2 0.47490152299114996 2315 3 0.4814647306631805 2315 4 0.2846825060156064 2315 5 0.36462665422380525 2315 6 0.3971034188791833 2315 7 0.3660558900824995 2315 8 0.4146198173995259 2315 9 0.3348153050330893 2315 10-11 0.5688434708041328 2315 12-13 0.5362487687085462 2315 14-15 0.7811307247088237 2315 16-17 0.5400154641900556 2315 18-19 0.7576217588087886 2315 20-21 0.6355959605395398 2315 22-23 0.5016918446410088 2315 24-25 0.7594880445726631 2315 26-27 0.7607395423125496 2315 28-29 0.7029153993344721 2315 30-31 0.8063201390882853 2315 32-33 0.7671864454360886 2315 34-35 0.6893980289790917 2315 36-37 0.7995290466369269 2315 38-39 0.8417819749946887 2315 40-41 0.9550136789417536 2315 42-43 0.804016547089411 2315 44-45 0.744763049253315 2315 46-47 0.8856400455428073 2315 48-49 0.8157622393679986 2315 50-51 0.8267925573634329 2315 52-53 0.517738388127178 2315 54-55 0.7558880945030992 2315 56-57 0.7690972230215181 2315 58-59 0.7604284344663697 2315 60-61 0.725336380192978 2315 62-63 0.7630697797094257 2315 64-65 0.8221559442657522 2315 66-67 0.6780458688266435 2315 68-69 0.8372389826889375 2315 70-71 0.7427748258742781 2315 72-73 0.8046845914329097 2315 74-75 0.6377511884533078 2315 76-77 0.8210655753214375 2315 78-79 0.8522880171121194 2315 80-81 0.8210290852231559 2315 82-83 0.7991907510900731 2315 84-85 0.8525246379449349 2315 86-87 0.9149232060170718 2315 88-89 0.7918918116407276 2315 90-91 0.878745672191851 2315 92-93 0.970547849066481 2315 94-95 1.010572313061747 2315 96-97 1.0327959073282535 2315 98-99 1.1994898469705397 2315 100 1.2222900752324506 2316 1 0.09173260899953917 2316 2 0.12560559763590362 2316 3 0.17720818212697154 2316 4 0.04467223380194696 2316 5 0.04125950818716717 2316 6 -0.01660998635964006 2316 7 -0.04367703236228948 2316 8 0.031277923888140435 2316 9 0.052691096849557084 2316 10-11 0.008621548188145312 2316 12-13 0.032132393087934474 2316 14-15 -0.05068638708710438 2316 16-17 0.1196732710724433 2316 18-19 0.19225730922823914 2316 20-21 0.20698998553525172 2316 22-23 0.17363800112105565 2316 24-25 0.1282027338381937 2316 26-27 -0.07367451349981025 2316 28-29 -0.11289096943799848 2316 30-31 -0.525891810920271 2316 32-33 -0.34323236127509205 2316 34-35 -0.5064834553557844 2316 36-37 -0.16125998777498296 2316 38-39 -0.05411341962952321 2316 40-41 0.28766754814223106 2316 42-43 0.30211404751742776 2316 44-45 0.05187869725879324 2316 46-47 0.06293417326066475 2316 48-49 -0.02190896419303101 2316 50-51 0.13841727915764324 2316 52-53 -0.10143427066412869 2316 54-55 -0.07011879644023367 2316 56-57 -0.05853610175163482 2316 58-59 -0.3956503619725993 2316 60-61 0.07170258461002987 2316 62-63 0.1506157906664498 2316 64-65 -0.24472044676317495 2316 66-67 -0.5303334754637206 2316 68-69 -0.651731227891446 2316 70-71 -0.36933889609113635 2316 72-73 -0.6190827429276524 2316 74-75 -0.34034759600140774 2316 76-77 0.028279665374508767 2316 78-79 0.16503412223110203 2316 80-81 0.1479626154405871 2316 82-83 0.40675473842101084 2316 84-85 0.39632920908001523 2316 86-87 0.46925037602220954 2316 88-89 0.5075590775198613 2316 90-91 -0.1216491660207808 2316 92-93 -0.2832432397881668 2316 94-95 0.04142105471557045 2316 96-97 0.4434522589803649 2316 98-99 0.5962979705795171 2316 100 0.6065564679734194 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 57.0 1 156.0 2 93.0 3 75.0 4 88.0 5 96.0 6 102.0 7 597.0 8 1822.0 9 2611.0 10 3046.0 11 3749.0 12 4742.0 13 5617.0 14 6278.0 15 7496.0 16 9338.0 17 12176.0 18 15301.0 19 18589.0 20 21640.0 21 25452.0 22 28164.0 23 27778.0 24 27177.0 25 27260.0 26 28431.0 27 32299.0 28 37411.0 29 44598.0 30 53092.0 31 65220.0 32 81234.0 33 105666.0 34 145702.0 35 203680.0 36 287799.0 37 414386.0 38 383473.0 39 94889.0 40 164.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.18288735860104 17.83440920471731 11.467998663692304 23.514704772989344 2 16.958058536037303 20.382630189315208 39.27087242289759 23.3884388517499 3 19.031008256625753 26.520835082555035 30.288422898722644 24.159733762096568 4 12.454628723318278 16.120340520299212 37.345881338733285 34.07914941764923 5 12.549188822216195 37.11001413477064 34.418525709297874 15.92227133371529 6 29.97196979870444 35.94539837154456 19.159390386911994 14.923241442839005 7 26.685665009364428 33.08869037192736 22.69547408275732 17.530170535950884 8 26.480165878277788 34.30131589761139 21.03693683862122 18.181581385489604 9 27.554219329227358 15.078800180525276 20.89315104172514 36.47382944852223 10-11 25.20882943023657 25.518427579114473 28.808637671591992 20.46410531905697 12-13 25.324958331190857 24.029705600292438 28.66722008024572 21.978115988270986 14-15 22.928487166165144 25.142452221419408 27.134246147200102 24.794814465215342 16-17 21.87220656043597 27.515007808438952 27.948463180207096 22.664322450917986 18-19 21.377507028211646 26.615702776255873 30.375257102311004 21.63153309322148 20-21 22.516752750954414 26.092948573995063 30.110172917134513 21.280125757916014 22-23 22.29266707553227 26.014451924344446 30.02979359173909 21.6630874083842 24-25 21.69905317910714 25.739876491466397 30.557086646892007 22.003983682534464 26-27 21.612931190921522 26.005603078863537 30.25505373098394 22.126411999231003 28-29 21.577055152531997 26.683538186279453 30.125319204315243 21.61408745687331 30-31 22.395611571925702 26.232476918848125 30.189890644116396 21.182020865109777 32-33 22.08305197749273 26.322780402767577 29.9489640902743 21.645203529465395 34-35 22.171256363558786 26.526254802844768 29.969886079104626 21.332602754491823 36-37 21.992787770062847 26.52278584563385 29.81030339734698 21.674122986956323 38-39 22.02368093304475 26.76545923520907 29.66316897647761 21.547690855268563 40-41 22.15256153857383 26.286642134707183 29.901509086070345 21.65928724064864 42-43 21.853758340576242 26.63044088549913 29.438107365759116 22.077693408165512 44-45 21.55655633398869 26.9336741729541 29.57641648553386 21.93335300752336 46-47 21.54174894394604 26.523282598422792 29.254109671495797 22.68085878613537 48-49 21.71333357140114 26.695239295202505 29.21006631535944 22.381360818036917 50-51 21.785656296298292 27.062693151676154 28.967007824095624 22.184642727929926 52-53 21.661203692931313 27.344478189056776 28.674390744043997 22.319927373967914 54-55 21.50269527856901 26.941736889430253 29.089179092076034 22.466388739924707 56-57 21.822138099577835 27.02803520874029 28.634612525540348 22.515214166141522 58-59 21.548061621713394 27.126044781247767 28.995950005906334 22.329943591132505 60-61 21.958127921179223 27.188286471328905 28.751061915244847 22.102523692247026 62-63 22.032907099193594 27.094176432052606 28.690594978302013 22.182321490451784 64-65 21.665439344338136 26.856709612842128 28.705845310674245 22.77200573214549 66-67 21.609441346854336 26.81215758454445 28.56453422515183 23.01386684344938 68-69 21.314571746584726 26.905002960039027 28.752444614929917 23.027980678446333 70-71 21.424455086829465 26.659758399294258 28.900431115411184 23.01535539846509 72-73 21.452613966232644 27.11113357027754 28.39029539418644 23.045957069303373 74-75 21.598002749206643 27.012134282193646 28.286668458748764 23.10319450985095 76-77 21.41093453039028 26.806224498480884 28.387927834336157 23.39491313679268 78-79 21.23435090141009 26.80818243949966 28.637550020629266 23.319916638460988 80-81 21.369086642953608 26.885708131728535 28.391188660315485 23.35401656500237 82-83 21.7596039204616 26.775208557768597 28.513783216699984 22.95140430506982 84-85 21.788453518135018 26.93473900475427 28.134838990627784 23.14196848648293 86-87 21.314030870286228 27.051408854321068 28.418468271806564 23.21609200358614 88-89 21.289188401252932 26.58520118034065 28.508330799047858 23.61727961935856 90-91 21.783201884790834 26.43698118106701 28.505484765588236 23.274332168553922 92-93 21.825085115516078 26.53782403926781 28.323739282924944 23.31335156229117 94-95 21.49642275509631 26.3301622957264 28.481248947043298 23.692166002133995 96-97 21.451668986263016 26.23189434500679 28.472768178432517 23.843668490297677 98-99 21.483376476162043 26.241667683179553 28.25917057490599 24.015785265752413 100 21.619913553787956 26.285186197373534 28.146395797074348 23.948504451764162 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1970.0 1 1477.0 2 2822.5 3 3945.0 4 2336.0 5 1677.0 6 2424.0 7 3938.0 8 5647.5 9 6143.0 10 4887.5 11 3655.5 12 3566.0 13 3785.5 14 4052.5 15 4278.5 16 4480.5 17 4754.5 18 5149.5 19 5503.0 20 5840.0 21 6359.5 22 7162.5 23 8303.5 24 9603.0 25 11199.5 26 13291.5 27 15569.5 28 17909.5 29 20879.0 30 24646.0 31 28082.0 32 31964.0 33 36515.0 34 40543.0 35 44557.5 36 49281.0 37 54363.5 38 57366.5 39 58608.0 40 59667.5 41 60733.5 42 62488.5 43 65696.5 44 68977.0 45 73371.5 46 78249.5 47 81337.0 48 84799.5 49 88437.5 50 90499.5 51 90018.5 52 86789.5 53 82650.0 54 79252.5 55 73382.5 56 65640.0 57 58395.5 58 51261.5 59 44343.0 60 36222.5 61 28398.0 62 22684.0 63 18099.0 64 13731.0 65 10152.5 66 7612.5 67 5893.5 68 4720.0 69 3662.0 70 2930.0 71 2340.5 72 1826.5 73 1424.0 74 1102.5 75 872.0 76 664.0 77 447.5 78 299.5 79 229.0 80 165.0 81 123.5 82 93.5 83 59.5 84 51.5 85 45.0 86 36.5 87 28.5 88 21.5 89 14.5 90 12.0 91 11.0 92 8.5 93 6.0 94 4.0 95 3.5 96 3.5 97 3.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022984955628261437 2 0.10859493684524302 3 0.016295974400505668 4 0.016340866891967117 5 0.01849570648211663 6 0.04565566381629275 7 0.04641883617113736 8 0.03820351023369235 9 0.03182877644616672 10-11 0.03530794453442895 12-13 0.034050954773508404 14-15 0.029494366890171417 16-17 0.03530794453442895 18-19 0.036093563135004295 20-21 0.04686776108575184 22-23 0.047518702211942834 24-25 0.0656777150080986 26-27 0.056923679173116214 28-29 0.04980821927647669 30-31 0.04722690101744342 32-33 0.043747732929181195 34-35 0.04484759896998668 36-37 0.040829720984187065 38-39 0.046104588730907226 40-41 0.05371386603362268 42-43 0.05517287200611974 44-45 0.05732771159626925 46-47 0.0597967986266489 48-49 0.05779908275661447 50-51 0.05173859640931896 52-53 0.06038040101564772 54-55 0.04749625596621212 56-57 0.052299752552587064 58-59 0.05032448292828335 60-61 0.045992357502253604 62-63 0.05322004862754675 64-65 0.05173859640931896 66-67 0.04857367576128687 68-69 0.05485862456588961 70-71 0.0550381945317354 72-73 0.05752972780784577 74-75 0.048641014498479046 76-77 0.048641014498479046 78-79 0.06004370732968687 80-81 0.060357954769917004 82-83 0.050391821665475525 84-85 0.055195318251850464 86-87 0.05369141978789195 88-89 0.0631637354862575 90-91 0.06798967831836318 92-93 0.07176064760112483 94-95 0.07492556824915692 96-97 0.07101992149201093 98-99 0.080694253401953 100 0.12605811602374634 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2227544.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.96246732908594 #Duplication Level Percentage of deduplicated Percentage of total 1 77.84327369597382 51.34734397959768 2 14.749864691495851 19.458749356424665 3 3.810899173646578 7.54128936708309 4 1.3660702282193 3.604374511926102 5 0.6779284584404839 2.2358916895669005 6 0.39717565614830164 1.571921174555437 7 0.2424667049839055 1.119559147412439 8 0.1778620491195959 0.93857756833085 9 0.12494582621605191 0.7417561481713777 >10 0.5734285079163932 6.384478224507298 >50 0.023707874073069752 1.0604030739423513 >100 0.01105984035337779 1.3350250536637105 >500 5.854637392383287E-4 0.24860744846893 >1k 4.3909780442873494E-4 0.6012921122580428 >5k 1.4636593480957832E-4 0.6513997882795464 >10k+ 1.4636593480957832E-4 1.1593313558115892 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12891 0.5787091074295277 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11125 0.49942896750861043 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7490 0.33624476104624645 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6004 0.26953451873453455 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3198 0.1435661876937111 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2531 0.1136228958889252 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2529 0.1135331109060023 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.4892491461448126E-5 2 0.0 0.0 0.0 0.0 4.4892491461448126E-5 3 0.0 0.0 0.0 0.0 4.4892491461448126E-5 4 0.0 0.0 0.0 0.0 4.4892491461448126E-5 5 0.0 0.0 0.0 0.0 4.4892491461448126E-5 6 0.0 0.0 0.0 0.0 4.4892491461448126E-5 7 0.0 0.0 0.0 0.0 4.4892491461448126E-5 8 0.0 0.0 0.0 0.0 4.4892491461448126E-5 9 0.0 0.0 0.0 0.0 8.978498292289625E-5 10-11 0.0 0.0 0.0 0.0 8.978498292289625E-5 12-13 0.0 0.0 0.0 0.0 8.978498292289625E-5 14-15 0.0 0.0 0.0 0.0 8.978498292289625E-5 16-17 0.0 0.0 0.0 0.0 8.978498292289625E-5 18-19 0.0 0.0 0.0 0.0 1.3467747438434437E-4 20-21 0.0 0.0 0.0 2.0201621157651657E-4 1.3467747438434437E-4 22-23 0.0 0.0 0.0 4.938174060759294E-4 1.5712372011506844E-4 24-25 0.0 0.0 0.0 0.0013692209895741678 1.795699658457925E-4 26-27 0.0 0.0 0.0 0.0034342755968007816 1.795699658457925E-4 28-29 0.0 0.0 0.0 0.012704575083589818 1.795699658457925E-4 30-31 0.0 0.0 0.0 0.04399464163221916 1.795699658457925E-4 32-33 0.0 0.0 0.0 0.10608095732340192 2.244624573072406E-4 34-35 0.0 0.0 0.0 0.19276835833545825 2.244624573072406E-4 36-37 0.0 0.0 0.0 0.3294659948355678 2.244624573072406E-4 38-39 0.0 2.2446245730724063E-5 0.0 0.5314373139206229 2.4690870303796465E-4 40-41 0.0 4.4892491461448126E-5 0.0 0.760097219179509 2.6935494876868875E-4 42-43 0.0 4.4892491461448126E-5 0.0 0.9653007976497883 2.6935494876868875E-4 44-45 0.0 4.4892491461448126E-5 0.0 1.2077875902788002 2.9180119449941284E-4 46-47 0.0 4.4892491461448126E-5 0.0 1.4922264161785357 3.142474402301369E-4 48-49 0.0 4.4892491461448126E-5 0.0 1.7881577198924017 4.489249146144812E-4 50-51 0.0 4.4892491461448126E-5 0.0 2.089947493741987 4.938174060759294E-4 52-53 0.0 4.4892491461448126E-5 0.0 2.3876744971143107 5.836023889988257E-4 54-55 0.0 1.3467747438434437E-4 0.0 2.7277800124262415 8.080648463060662E-4 56-57 0.0 1.795699658457925E-4 0.0 3.0999612128873775 8.080648463060662E-4 58-59 0.0 3.142474402301369E-4 0.0 3.5071585566884425 8.080648463060662E-4 60-61 0.0 3.142474402301369E-4 0.0 3.918486009703961 8.080648463060662E-4 62-63 0.0 3.142474402301369E-4 0.0 4.339195993434922 8.080648463060662E-4 64-65 0.0 3.142474402301369E-4 0.0 4.752835409760705 8.080648463060662E-4 66-67 4.4892491461448126E-5 3.142474402301369E-4 0.0 5.223420951505335 8.080648463060662E-4 68-69 4.4892491461448126E-5 3.142474402301369E-4 0.0 5.749448720204853 8.080648463060662E-4 70-71 4.4892491461448126E-5 3.142474402301369E-4 0.0 6.274623531566604 8.080648463060662E-4 72-73 4.4892491461448126E-5 3.142474402301369E-4 0.0 6.763300747370198 8.978498292289624E-4 74-75 4.4892491461448126E-5 3.142474402301369E-4 0.0 7.251439253276255 8.978498292289624E-4 76-77 4.4892491461448126E-5 3.142474402301369E-4 0.0 7.800429531358303 8.978498292289624E-4 78-79 4.4892491461448126E-5 3.142474402301369E-4 0.0 8.389508804315426 8.978498292289624E-4 80-81 4.4892491461448126E-5 3.142474402301369E-4 0.0 8.968195465499221 9.202960749596866E-4 82-83 4.4892491461448126E-5 3.366936859608609E-4 0.0 9.551057128388933 9.427423206904106E-4 84-85 4.4892491461448126E-5 3.59139931691585E-4 0.0 10.125434110392433 9.427423206904106E-4 86-87 4.4892491461448126E-5 3.59139931691585E-4 0.0 10.72863207191418 9.427423206904106E-4 88 4.4892491461448126E-5 3.59139931691585E-4 0.0 11.18527849506003 9.876348121518588E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4355 0.0 59.628567 1 TACCTGG 2225 0.0 48.8389 2 GTATCAA 8105 0.0 43.067966 1 TAACGCA 250 0.0 41.351223 4 ACCTGGG 2625 0.0 40.098156 3 GTACCTG 3040 0.0 35.747185 1 TATCAAC 10605 0.0 32.38144 2 ATCAACG 10590 0.0 32.2585 3 CAACGCA 10640 0.0 32.0193 5 TCAACGC 10900 0.0 31.384165 4 AACGCAG 11200 0.0 30.753294 6 GTACATG 8690 0.0 29.937038 1 ATAACGC 300 0.0 29.760351 3 TACATGG 8870 0.0 28.74482 2 GTGGTAT 1660 0.0 28.056309 1 ACGCAGA 12320 0.0 27.729317 7 CGCAGAG 12610 0.0 27.016472 8 ACATGGG 9090 0.0 26.7259 3 CATGGGG 6155 0.0 26.720568 4 CCTGGGG 2660 0.0 26.321484 4 >>END_MODULE