FastQCFastQC Report
Mon 6 Jun 2016
ERR1211351_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211351_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227544
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111420.500192139863455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99990.44888002212301975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71060.31900604432505036No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46780.21000707505665434No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26920.12085058701421834No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25530.11461053070107707No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24570.11030085152077805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46200.060.6418151
TACCTGG23650.048.994762
ACCTGGG27500.041.968673
GTATCAA83450.040.321131
GTACCTG32500.036.099111
CCTGGGG25500.034.0372434
TATCACG1554.1836756E-1133.2923772
ATCAACG107400.030.9283
CAACGCA108750.030.4495745
TATCAAC110250.030.1683442
TCAACGC110200.030.142174
AACGCAG114050.029.158596
GTACATG81450.027.8865071
TACATGG82850.026.952452
CTGGGGG18850.026.6243025
TAACGCA2500.026.2730714
ACATGGG83900.026.2260993
CATGGGG56400.025.7040864
TGGGGGG41100.025.6777086
ACGCAGA128750.025.5743987
Produced by FastQC (version 0.11.5)