##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211351_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2227544 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08742094432254 33.0 31.0 34.0 30.0 34.0 2 32.18954642422327 33.0 31.0 34.0 30.0 34.0 3 32.255097542405444 34.0 31.0 34.0 30.0 34.0 4 35.9114805364114 37.0 35.0 37.0 35.0 37.0 5 35.83226324597853 37.0 35.0 37.0 35.0 37.0 6 35.852843310839205 37.0 35.0 37.0 35.0 37.0 7 35.76642212230151 37.0 35.0 37.0 33.0 37.0 8 35.75075060245724 37.0 35.0 37.0 33.0 37.0 9 37.36227701899491 39.0 37.0 39.0 34.0 39.0 10-11 37.30611449201452 39.0 37.0 39.0 34.0 39.0 12-13 37.232938833082535 39.0 37.0 39.0 33.0 39.0 14-15 38.44329943650945 40.0 38.0 41.0 34.0 41.0 16-17 38.424547393901086 40.0 38.0 41.0 33.5 41.0 18-19 38.44824120196952 40.0 38.0 41.0 34.0 41.0 20-21 38.418614626692 40.0 38.0 41.0 34.0 41.0 22-23 38.295887533534696 40.0 38.0 41.0 34.0 41.0 24-25 38.197289032225626 40.0 37.0 41.0 33.0 41.0 26-27 38.078045147480815 40.0 37.0 41.0 33.0 41.0 28-29 37.95115539805275 40.0 37.0 41.0 33.0 41.0 30-31 37.909875405379196 40.0 37.0 41.0 33.0 41.0 32-33 37.78002432275187 40.0 37.0 41.0 33.0 41.0 34-35 37.60056838383439 40.0 36.0 41.0 33.0 41.0 36-37 37.41756616255391 40.0 36.0 41.0 32.0 41.0 38-39 37.28710027725603 40.0 36.0 41.0 31.5 41.0 40-41 37.152303388844395 39.5 36.0 41.0 31.0 41.0 42-43 37.022309099169306 39.0 35.5 41.0 31.0 41.0 44-45 36.83861845153227 39.0 35.0 41.0 30.5 41.0 46-47 36.56031665367777 39.0 35.0 41.0 30.0 41.0 48-49 34.985394452365476 39.0 34.5 40.0 22.0 41.0 50-51 35.710538153230644 38.5 34.5 40.0 27.0 41.0 52-53 35.813731849965706 38.5 35.0 40.0 28.0 41.0 54-55 35.64748148633652 38.0 35.0 40.0 27.0 41.0 56-57 35.43919334477792 38.0 34.0 40.0 27.0 41.0 58-59 35.2111834827954 38.0 34.0 40.0 26.5 41.0 60-61 34.9573790686065 37.5 34.0 40.0 26.0 41.0 62-63 34.63038126295149 37.0 34.0 40.0 26.0 41.0 64-65 34.298436529199876 36.0 33.0 39.0 26.0 41.0 66-67 33.963700380329186 36.0 33.0 39.0 25.0 40.5 68-69 33.60537794090712 35.0 32.5 39.0 25.0 40.0 70-71 33.237592613209884 35.0 32.0 38.0 24.0 40.0 72-73 32.85515819216141 35.0 32.0 37.0 23.5 39.5 74-75 32.48282458169176 35.0 31.5 37.0 23.0 39.0 76-77 31.538466580233656 34.0 30.5 36.0 21.5 38.0 78-79 31.961460245005263 34.5 31.0 36.0 23.0 38.0 80-81 31.838629899117592 34.5 31.0 35.5 23.0 37.0 82-83 31.559649326792197 34.0 31.0 35.0 21.5 37.0 84-85 31.267471484289423 34.0 31.0 35.0 20.5 36.5 86-87 30.994282043362556 34.0 30.5 35.0 20.0 36.0 88-89 30.764747632370003 34.0 30.0 35.0 20.0 36.0 90-91 30.539017860028803 34.0 30.0 35.0 19.0 35.0 92-93 30.331204681029867 34.0 29.5 35.0 18.5 35.0 94-95 30.129513042166618 34.0 29.0 35.0 18.0 35.0 96-97 29.935346282722136 34.0 29.0 35.0 18.0 35.0 98-99 29.704283506857777 34.0 29.0 35.0 17.0 35.0 100 27.365672687049056 31.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.25737268143298664 1101 2 -0.31659891680802943 1101 3 -0.31457000487997533 1101 4 -0.17148559378875206 1101 5 -0.14965292670026997 1101 6 -0.2765430162840232 1101 7 -0.3491828025085155 1101 8 -0.26143093405611495 1101 9 -0.4361806972148088 1101 10-11 -0.44497378028444956 1101 12-13 -0.3360699516141139 1101 14-15 -0.4600396724695557 1101 16-17 -0.5331789429259501 1101 18-19 -0.5563198824880828 1101 20-21 -0.5535484715628769 1101 22-23 -0.7189326600299424 1101 24-25 -0.604677166894966 1101 26-27 -0.6799450044368882 1101 28-29 -0.7696249267907618 1101 30-31 -0.595659609049882 1101 32-33 -0.7921457127692833 1101 34-35 -0.8219002944163947 1101 36-37 -0.7866411363717845 1101 38-39 -0.7122920350081614 1101 40-41 -0.8119071270504961 1101 42-43 -0.7737337180525259 1101 44-45 -0.7030457685703055 1101 46-47 -0.8177938858371334 1101 48-49 0.22433199749666244 1101 50-51 -0.6150125800629453 1101 52-53 -0.7054460645225191 1101 54-55 -0.6828051336514207 1101 56-57 -0.7659902543502852 1101 58-59 -0.9348520051699225 1101 60-61 -0.8906886668617346 1101 62-63 -0.901078322117769 1101 64-65 -1.0629144862620379 1101 66-67 -0.8526199989070946 1101 68-69 -0.9101970602959994 1101 70-71 -0.8186865691161245 1101 72-73 -0.9959222856658059 1101 74-75 -1.006115680058116 1101 76-77 -0.9771622369574793 1101 78-79 -0.7521242772742518 1101 80-81 -0.8318724741115346 1101 82-83 -0.825805270733813 1101 84-85 -0.8198285843959034 1101 86-87 -0.9657931492444227 1101 88-89 -0.8911893084025984 1101 90-91 -0.9393918918776087 1101 92-93 -0.9905926086370975 1101 94-95 -1.091839741138788 1101 96-97 -0.9535541382308352 1101 98-99 -1.1415052892798627 1101 100 -1.280553929949292 1102 1 -0.25610054148990713 1102 2 -0.24034079813115383 1102 3 -0.3567285139561207 1102 4 -0.2797536523972326 1102 5 -0.14818285561801048 1102 6 -0.13665593860689285 1102 7 -0.19855857820778766 1102 8 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-0.7531562632781998 1102 72-73 -0.7527690971214369 1102 74-75 -0.7053376267506763 1102 76-77 -0.8791392423554285 1102 78-79 -0.7182430507820072 1102 80-81 -0.721940668389319 1102 82-83 -0.8562421934289368 1102 84-85 -0.6307749725094958 1102 86-87 -0.7971452495244051 1102 88-89 -0.65517042560721 1102 90-91 -0.544609999090401 1102 92-93 -1.0020805758788072 1102 94-95 -0.9877485511608555 1102 96-97 -0.897878387320997 1102 98-99 -1.1148518436446295 1102 100 -1.2257937135043164 1103 1 -0.3833325283617697 1103 2 -0.29962015875083026 1103 3 -0.36321287075091746 1103 4 -0.19592173870157126 1103 5 -0.2674180713828278 1103 6 -0.19762228419993733 1103 7 -0.22726973922490856 1103 8 -0.3220545515897584 1103 9 -0.5035232778851721 1103 10-11 -0.3606773789432651 1103 12-13 -0.44784970794259493 1103 14-15 -0.4647412010482199 1103 16-17 -0.56698060037548 1103 18-19 -0.646334565067022 1103 20-21 -0.6572817645530549 1103 22-23 -0.6922711304648672 1103 24-25 -0.674948643595549 1103 26-27 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0.35377205275466395 2311 2 0.396664948325423 2311 3 0.49567743172590184 2311 4 0.2746940937797717 2311 5 0.3649380266455182 2311 6 0.3898868269739708 2311 7 0.3796894795043073 2311 8 0.2804561099491849 2311 9 0.5349312325451265 2311 10-11 0.4038276301305217 2311 12-13 0.5044448323064543 2311 14-15 0.5300795286461408 2311 16-17 0.567137698742691 2311 18-19 0.7184237781001102 2311 20-21 0.67294022669779 2311 22-23 0.7093712458020534 2311 24-25 0.6549448804872569 2311 26-27 0.7147519054561897 2311 28-29 0.722687083664546 2311 30-31 0.6545794071681357 2311 32-33 0.7058851444437408 2311 34-35 0.7741666827520675 2311 36-37 0.8994275073317795 2311 38-39 0.8413309245827492 2311 40-41 0.7491669986764222 2311 42-43 0.8367948367106948 2311 44-45 0.6946026626938959 2311 46-47 0.7753873542404079 2311 48-49 -5.8536377241919695 2311 50-51 -1.1318730910752421 2311 52-53 0.23006560560878597 2311 54-55 0.7128193345923179 2311 56-57 0.8662076423069962 2311 58-59 0.8700888328340994 2311 60-61 0.6694776357985859 2311 62-63 0.487268211927784 2311 64-65 0.6039210267237749 2311 66-67 0.882960086875606 2311 68-69 0.9138102549159584 2311 70-71 0.9025613148228615 2311 72-73 0.3947639759451178 2311 74-75 0.7540984258458039 2311 76-77 0.8091616497532179 2311 78-79 0.7306402413163795 2311 80-81 0.7789279890098015 2311 82-83 0.7791623195441524 2311 84-85 0.7807402303467903 2311 86-87 0.7484289735704444 2311 88-89 0.6960231046525074 2311 90-91 0.8130399336800203 2311 92-93 0.726828823004265 2311 94-95 0.8373390956197149 2311 96-97 0.8636094190321906 2311 98-99 0.9460711301256239 2311 100 1.0868556605216355 2312 1 -0.026293333011416564 2312 2 0.10908975032574375 2312 3 0.19631128378872376 2312 4 -0.06093189915620201 2312 5 0.1349797039702878 2312 6 0.07493333546987202 2312 7 0.17082440852510672 2312 8 0.29942888877796037 2312 9 0.23950926055356092 2312 10-11 0.15639152526864564 2312 12-13 0.1905275494282037 2312 14-15 0.30258288877310946 2312 16-17 0.413275518632652 2312 18-19 0.36779803071477346 2312 20-21 0.3321969132826865 2312 22-23 0.6100163359492612 2312 24-25 0.7008392738280449 2312 26-27 0.6446059218360745 2312 28-29 0.7352437599152211 2312 30-31 0.6765341951193378 2312 32-33 0.6171256692773497 2312 34-35 0.39958747067737477 2312 36-37 0.719720143433868 2312 38-39 0.7656379068678518 2312 40-41 0.44000729706112196 2312 42-43 0.5026538225534125 2312 44-45 0.6394093570972359 2312 46-47 0.7195274464529575 2312 48-49 -4.591620204831884 2312 50-51 -0.8495991505659859 2312 52-53 0.3816657822847347 2312 54-55 0.5252000429290788 2312 56-57 0.6524375090377745 2312 58-59 0.6352767423425121 2312 60-61 0.7354244022281051 2312 62-63 0.5554163473548357 2312 64-65 0.6997400827186624 2312 66-67 0.767175052448799 2312 68-69 0.6271174574434113 2312 70-71 0.5671914929660815 2312 72-73 0.6982537928246018 2312 74-75 0.38917214074975703 2312 76-77 0.6720021855537475 2312 78-79 0.3405229464468249 2312 80-81 0.6057800659915813 2312 82-83 0.6195142583848643 2312 84-85 0.6589251953565771 2312 86-87 0.6867956882324684 2312 88-89 0.7479151852979662 2312 90-91 0.6439127893676506 2312 92-93 0.7768735493749865 2312 94-95 0.8340423146831206 2312 96-97 0.9112842051395056 2312 98-99 0.9303112985341926 2312 100 0.4630073005438007 2313 1 0.0433566700355712 2313 2 0.20627709344231704 2313 3 0.2953733929404194 2313 4 0.08275112159520859 2313 5 0.12986468756010083 2313 6 0.2558920038505832 2313 7 0.21438482442398055 2313 8 0.28140931262801416 2313 9 0.49862119515656644 2313 10-11 0.25568165042716373 2313 12-13 0.12620723010422807 2313 14-15 0.16892459212451172 2313 16-17 0.11955049302347476 2313 18-19 0.4204760399021623 2313 20-21 0.48574991485260455 2313 22-23 0.49564944129077304 2313 24-25 0.3121514214297747 2313 26-27 0.5335057572989115 2313 28-29 0.48301721226473404 2313 30-31 0.6022487855320762 2313 32-33 0.6166107288966813 2313 34-35 0.399038287994685 2313 36-37 -0.2433834134127082 2313 38-39 0.23144863689631734 2313 40-41 0.45667655124637463 2313 42-43 0.5526890805169273 2313 44-45 0.5825910763509867 2313 46-47 0.4156344884694221 2313 48-49 -5.084309690994225 2313 50-51 -1.249400516195415 2313 52-53 0.17494522268426493 2313 54-55 0.4353962687309405 2313 56-57 0.45265326455935195 2313 58-59 0.4649550968548013 2313 60-61 0.6890922460948161 2313 62-63 0.7165401622955088 2313 64-65 0.705796617339665 2313 66-67 0.7583956018926514 2313 68-69 0.7609393481408233 2313 70-71 0.730615055732514 2313 72-73 0.5894104281411856 2313 74-75 0.6499261098098117 2313 76-77 0.6821165780575846 2313 78-79 0.6779593587821822 2313 80-81 -0.08039757900860423 2313 82-83 0.30861504973026754 2313 84-85 0.6576065765401431 2313 86-87 0.85073905204532 2313 88-89 0.7314241071587766 2313 90-91 0.7308785876921 2313 92-93 0.840528030038243 2313 94-95 0.613160822066984 2313 96-97 0.5632786741371625 2313 98-99 0.756523490534839 2313 100 1.136436580033532 2314 1 0.16750068061397627 2314 2 0.23179907311551773 2314 3 0.2233889972347427 2314 4 0.1108852177797175 2314 5 0.21579148590350172 2314 6 0.26139500764862333 2314 7 0.07892669871381486 2314 8 0.26041621320644737 2314 9 0.4345218050749722 2314 10-11 0.2683780993912137 2314 12-13 0.18201884230946064 2314 14-15 0.29420214649768894 2314 16-17 0.44408926190990883 2314 18-19 0.470171080701725 2314 20-21 0.5793197978776377 2314 22-23 0.4281161476455466 2314 24-25 0.4457224063721483 2314 26-27 0.5293675568300955 2314 28-29 0.591492888512839 2314 30-31 0.503784815366842 2314 32-33 0.5479702945409031 2314 34-35 0.46787449156032324 2314 36-37 0.39301642230143585 2314 38-39 0.5111018763901853 2314 40-41 0.3052210901726724 2314 42-43 0.34939792533548086 2314 44-45 0.547990156464671 2314 46-47 0.7212526211631669 2314 48-49 -5.946838562659494 2314 50-51 -1.3027554967186674 2314 52-53 -0.5331985904076575 2314 54-55 -0.04496656259556886 2314 56-57 0.15292387828437626 2314 58-59 0.6282648272141955 2314 60-61 0.7318921464929389 2314 62-63 0.6499707601071307 2314 64-65 0.7529223303660402 2314 66-67 0.7939581235374575 2314 68-69 0.8406788806416614 2314 70-71 0.577576626007648 2314 72-73 0.7685972131470962 2314 74-75 0.7853053840326751 2314 76-77 0.690188766522656 2314 78-79 0.42900990101136927 2314 80-81 0.5219716148172182 2314 82-83 0.4599686907799807 2314 84-85 0.5540429472537554 2314 86-87 0.628323835282437 2314 88-89 0.5533653563609597 2314 90-91 0.6975936003856162 2314 92-93 0.7594185423868716 2314 94-95 0.8022232907625089 2314 96-97 0.7745298225639985 2314 98-99 0.897304449403272 2314 100 -0.6250934822748846 2315 1 0.1757384719981019 2315 2 0.21713403806375453 2315 3 0.3272227904454823 2315 4 0.18702026904009728 2315 5 0.32559200019196055 2315 6 0.31742578179827774 2315 7 0.38791398631175156 2315 8 0.36689987519261535 2315 9 0.4888403872044833 2315 10-11 0.34477904079574273 2315 12-13 0.48968477991094517 2315 14-15 0.3588181112330133 2315 16-17 0.4392710514817679 2315 18-19 0.581689808787651 2315 20-21 0.3796155818449449 2315 22-23 0.48381299990482063 2315 24-25 0.4937636440767861 2315 26-27 0.598730259231715 2315 28-29 0.7411388581874547 2315 30-31 0.6111647902330972 2315 32-33 0.673152507070931 2315 34-35 0.7237448283568924 2315 36-37 0.6057418715511176 2315 38-39 0.6524067525243709 2315 40-41 0.6859025116275532 2315 42-43 0.6920176278740229 2315 44-45 0.6630740634798258 2315 46-47 0.5271027778369088 2315 48-49 -5.080113111976054 2315 50-51 -1.034891786744673 2315 52-53 0.18134858985926172 2315 54-55 0.18493041483065298 2315 56-57 0.5905184076430459 2315 58-59 0.680381374413102 2315 60-61 0.5637789628868006 2315 62-63 0.3371828707615947 2315 64-65 0.5638060476952091 2315 66-67 -0.20500548557176757 2315 68-69 0.5539186183502451 2315 70-71 0.5055262036588246 2315 72-73 0.6316990593236582 2315 74-75 0.6957539562621022 2315 76-77 0.6333923663984926 2315 78-79 0.718940342314454 2315 80-81 0.5585468866753729 2315 82-83 0.5516959157370955 2315 84-85 0.49592412181575796 2315 86-87 0.5186563735977856 2315 88-89 0.4463324939449471 2315 90-91 0.7376274513767349 2315 92-93 0.7828509984762881 2315 94-95 0.24660070709027693 2315 96-97 0.5755629158148032 2315 98-99 0.5203157395188818 2315 100 0.5198789430031034 2316 1 0.20695316126364105 2316 2 0.22495162066947216 2316 3 0.11932816623729536 2316 4 -0.048181323153023925 2316 5 0.1505556194352451 2316 6 0.15304682271592895 2316 7 0.2472938014119066 2316 8 0.279481196884106 2316 9 0.4433665813493235 2316 10-11 0.3584143344098436 2316 12-13 0.2845904039479308 2316 14-15 0.42542806672008737 2316 16-17 0.44263092136706206 2316 18-19 0.08054809504000815 2316 20-21 0.4498989237384521 2316 22-23 0.5211407263215264 2316 24-25 0.5961369534416221 2316 26-27 0.6256209885588788 2316 28-29 0.6541353485144512 2316 30-31 0.5674715014126903 2316 32-33 0.4929265183703677 2316 34-35 0.4113047496032607 2316 36-37 0.26824936170690705 2316 38-39 0.4718180262788678 2316 40-41 0.03257795247672135 2316 42-43 0.0192304339003897 2316 44-45 0.24764006245260362 2316 46-47 0.3541925313037808 2316 48-49 -4.998299210246898 2316 50-51 -1.0859222181776431 2316 52-53 0.23934058609278708 2316 54-55 0.4236737806926669 2316 56-57 0.36690622650967697 2316 58-59 0.22833656188100804 2316 60-61 0.37836229622013207 2316 62-63 0.3272316637764874 2316 64-65 0.33441788642145553 2316 66-67 0.34444880307743375 2316 68-69 0.008454442695388309 2316 70-71 -0.3394393416245123 2316 72-73 -0.2097921236929423 2316 74-75 0.11477146182621567 2316 76-77 0.32569569630775774 2316 78-79 0.383913163749142 2316 80-81 0.3073259912807558 2316 82-83 0.1887358061668145 2316 84-85 0.26553548973221197 2316 86-87 0.343080701623812 2316 88-89 0.08022223885164337 2316 90-91 0.21427742569620278 2316 92-93 0.3586984561034079 2316 94-95 0.4901046019712858 2316 96-97 0.580308464522215 2316 98-99 0.3343544315236784 2316 100 -0.22335209620251462 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 5.0 11 7.0 12 16.0 13 70.0 14 302.0 15 991.0 16 2262.0 17 4964.0 18 8267.0 19 11260.0 20 14544.0 21 18439.0 22 21878.0 23 22757.0 24 22338.0 25 23015.0 26 26137.0 27 31096.0 28 37679.0 29 45851.0 30 55984.0 31 68854.0 32 86342.0 33 111484.0 34 155351.0 35 216336.0 36 323497.0 37 475564.0 38 388789.0 39 53461.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.28853459595098 18.534667799478022 12.233778853908648 23.943018750662354 2 16.634193643966977 20.774863186815654 38.94450704604734 23.646436123170027 3 18.690638070735336 26.266339190758607 30.979693770221274 24.063328968284786 4 12.369524004529783 16.45337888262347 37.33187309780973 33.84522401503701 5 12.369962644773388 37.28739372211321 34.42709939650725 15.915544236606147 6 30.101627622170422 36.26680325955402 18.797967627126557 14.833601491148995 7 26.66270714536471 33.13302285658363 22.43059839051295 17.773671607538713 8 26.60432044836841 34.6718720117228 20.675264225434063 18.04854331447473 9 27.309562944448913 15.080385231332304 20.69879861017888 36.91125321403991 10-11 25.239430802606933 25.664544353648505 28.618498803042392 20.47752604070217 12-13 25.159121578852385 24.043721734539687 28.673174288071785 22.123982398536143 14-15 22.795032003943366 25.249445800700144 27.010111612012906 24.945410583343584 16-17 21.813956609972575 27.55425885826043 27.93580091396808 22.69598361779892 18-19 21.367905639574346 26.606275790736344 30.364136465991244 21.661682103698062 20-21 22.43061076710977 26.163512892904873 30.133043447421663 21.272832892563688 22-23 22.23646314689369 25.99689029083435 29.979019451731382 21.787627110540576 24-25 21.596440562539083 25.86856909159554 30.45795300023389 22.077037345631485 26-27 21.595799316017388 26.030552959270803 30.155705257184824 22.21794246752699 28-29 21.551403115250533 26.747595033143277 30.03954387952012 21.661457972086065 30-31 22.293842462557063 26.30147078134375 30.164541740825268 21.240145015273924 32-33 22.05363268047569 26.332758057158866 29.922382613130665 21.691226649234782 34-35 22.1512741027041 26.625877359981608 29.877452477082674 21.345396060231618 36-37 21.8861609448092 26.51463310154939 29.818744235710177 21.78046171793124 38-39 21.95867375426202 26.69309435411829 29.7442147993104 21.60401709230929 40-41 22.102638005962003 26.305287684516752 29.941143195776316 21.650931113744925 42-43 21.76270515299519 26.690933872910534 29.47394814545778 22.0724128286365 44-45 21.464427876555558 27.001563303931963 29.61520364705069 21.918805172461788 46-47 21.43920261305856 26.62962588813685 29.197517145390666 22.733654353413925 48-49 21.781771455254617 26.771858586248776 29.11000046581963 22.336369492676976 50-51 21.62129396936978 27.08263082769121 29.012341798722762 22.283733404216246 52-53 21.6159568413644 27.305851807200515 28.616887410156334 22.46130394127875 54-55 21.49722471772396 26.938533435158785 29.0925143478255 22.471727499291756 56-57 21.739749216399456 26.979922810604485 28.581761025348857 22.6985669476472 58-59 21.37909623822439 27.07390614709037 29.031835174884925 22.51516243980032 60-61 21.832844768971796 27.204583548546328 28.725653994970468 22.236917687511408 62-63 21.843312013835074 27.196771815521544 28.561333439885928 22.398582730757454 64-65 21.4391214907573 26.905266022087105 28.57007192868511 23.085540558470484 66-67 21.38529769483773 26.944751231119525 28.419490183038448 23.250460891004302 68-69 21.208717979575543 27.02922128950155 28.56184141633041 23.200219314592506 70-71 21.241990309790346 26.83471861121963 28.617057103983402 23.306233975006624 72-73 21.309957942526765 27.32390944790053 28.030285511713476 23.33584709785923 74-75 21.53762231545771 27.283547378369217 27.850076436638403 23.32875386953467 76-77 21.299457287334647 27.197615880659338 27.942439905937288 23.56048692606873 78-79 21.19798030453466 27.11770923990697 28.111416828535774 23.572893627022598 80-81 21.24832041819286 27.366644683767237 27.747701559917914 23.637333338121984 82-83 21.635722199086988 27.212012163022216 27.89644594156227 23.25581969632853 84-85 21.74470127693815 27.431831223962146 27.435894902934198 23.387572596165505 86-87 21.236660351149915 27.475349166354036 27.667194493893167 23.620795988602882 88-89 21.185876680967937 27.131134584312672 27.7376661658079 23.94532256891149 90-91 21.69310161815669 26.979388460878756 27.630234268569648 23.697275652394907 92-93 21.533285785898705 27.146846368132532 27.541772935632043 23.778094910336716 94-95 21.17118532742089 26.978355413705618 27.652394525797646 24.198064733075842 96-97 21.14906881793178 26.883458748725285 27.741968502752794 24.22550393059014 98-99 21.190823894153976 26.986425766013117 27.444265719587058 24.378484620245846 100 21.01815147651679 26.980803058792567 27.62680862472443 24.374236839966212 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2012.0 1 1518.0 2 2443.5 3 3386.5 4 2141.5 5 1516.0 6 2183.5 7 3706.5 8 5242.5 9 5696.0 10 4638.5 11 3471.0 12 3316.5 13 3527.0 14 3846.0 15 4080.0 16 4379.5 17 4698.0 18 5078.0 19 5473.0 20 5815.0 21 6291.5 22 7162.0 23 8315.0 24 9636.5 25 11284.0 26 13216.5 27 15385.5 28 17846.5 29 20931.0 30 24697.5 31 28178.0 32 32132.0 33 36537.0 34 40662.0 35 44615.0 36 49360.0 37 54564.5 38 57374.0 39 58659.5 40 59419.0 41 60259.5 42 62352.0 43 65709.0 44 68682.0 45 72886.5 46 77860.0 47 81135.0 48 84475.0 49 87861.5 50 90171.5 51 90901.5 52 89262.0 53 85814.0 54 81987.0 55 75207.5 56 66979.5 57 59620.0 58 52475.5 59 45207.0 60 36224.5 61 27993.0 62 22202.0 63 17313.0 64 12898.5 65 9328.5 66 6804.5 67 5341.5 68 4185.5 69 3118.5 70 2448.0 71 1900.5 72 1473.5 73 1162.5 74 906.5 75 662.0 76 461.0 77 319.0 78 196.5 79 112.5 80 74.5 81 50.0 82 33.5 83 24.0 84 15.5 85 7.0 86 3.5 87 3.0 88 1.5 89 1.0 90 1.0 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02855162456948101 2 0.002424194538918199 3 4.489249146144812E-5 4 0.02271560067949275 5 4.938174060759294E-4 6 0.0 7 8.978498292289624E-5 8 0.004040324231530331 9 0.009292745732519762 10-11 9.651885664211347E-4 12-13 8.978498292289624E-5 14-15 2.6935494876868875E-4 16-17 2.0201621157651655E-4 18-19 0.0 20-21 4.489249146144812E-5 22-23 2.0201621157651655E-4 24-25 4.489249146144812E-5 26-27 8.978498292289624E-5 28-29 8.305110920367903E-4 30-31 0.002446640784648923 32-33 0.00933763822398121 34-35 0.018316136516270835 36-37 0.021099470986880618 38-39 0.020672992317996863 40-41 0.004668819111990605 42-43 0.005431991466835223 44-45 0.009427423206904107 46-47 0.012076080203129546 48-49 1.2175517071716653 50-51 0.017822319110194905 52-53 0.018473260236385902 54-55 0.00911317576667397 56-57 0.007429707336869664 58-59 0.00740726109113894 60-61 0.0044668029004140885 62-63 0.007272583616754596 64-65 0.01829369027054011 66-67 0.027272188562829733 68-69 0.02720484982563756 70-71 0.024870440269642264 72-73 0.02121170221553424 74-75 0.009382530715442658 76-77 0.01375954863293385 78-79 0.00801330972586849 80-81 0.002469087030379647 82-83 0.01353508617562661 84-85 0.022356460747801166 86-87 0.021974874570378857 88-89 0.031514529005936584 90-91 0.01984248122596007 92-93 0.013220838735396472 94-95 0.006778766210678667 96-97 0.003568953071185126 98-99 0.015555248291391774 100 0.03515082081431388 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2227544.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.19400571061614 #Duplication Level Percentage of deduplicated Percentage of total 1 77.77673693657431 51.483537689326894 2 14.82516273956943 19.62673814087745 3 3.7870540413655833 7.5204083052169635 4 1.3825635347459029 3.6606967405703976 5 0.67983792409947 2.2500597715066872 6 0.40353638757445653 1.6027013966126995 7 0.24113506781789 1.1173187239317053 8 0.18329585584712535 0.9706469542941512 9 0.12045580721051499 0.7176107151332736 >10 0.564900118658262 6.302354766543126 >50 0.021962155529240013 0.9783997071055779 >100 0.012112597063938435 1.4059295284016913 >500 5.867453853416236E-4 0.2578425930719464 >1k 4.4005903900612176E-4 0.7343974591223276 >5k 1.466863463353739E-4 0.83042695433909 >10k+ 7.334317316768696E-5 0.5409305539459889 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11142 0.500192139863455 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9999 0.44888002212301975 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7106 0.31900604432505036 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4678 0.21000707505665434 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2692 0.12085058701421834 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2553 0.11461053070107707 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2457 0.11030085152077805 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.4892491461448126E-5 7 0.0 0.0 0.0 0.0 4.4892491461448126E-5 8 0.0 0.0 0.0 0.0 4.4892491461448126E-5 9 0.0 0.0 0.0 0.0 4.4892491461448126E-5 10-11 0.0 0.0 0.0 4.4892491461448126E-5 4.4892491461448126E-5 12-13 0.0 0.0 0.0 4.4892491461448126E-5 4.4892491461448126E-5 14-15 0.0 0.0 0.0 4.4892491461448126E-5 6.733873719217219E-5 16-17 0.0 0.0 0.0 4.4892491461448126E-5 8.978498292289625E-5 18-19 0.0 0.0 0.0 4.4892491461448126E-5 8.978498292289625E-5 20-21 0.0 0.0 0.0 2.469087030379647E-4 8.978498292289625E-5 22-23 0.0 0.0 0.0 5.387098975373775E-4 8.978498292289625E-5 24-25 0.0 0.0 0.0 0.001414113481035616 8.978498292289625E-5 26-27 0.0 0.0 0.0 0.0034567218425315058 8.978498292289625E-5 28-29 0.0 0.0 0.0 0.01277191382078199 8.978498292289625E-5 30-31 0.0 0.0 0.0 0.0443986740553722 8.978498292289625E-5 32-33 0.0 0.0 0.0 0.10684412967824652 8.978498292289625E-5 34-35 0.0 0.0 0.0 0.19326217574153418 8.978498292289625E-5 36-37 0.0 4.4892491461448126E-5 0.0 0.32978024227579794 8.978498292289625E-5 38-39 0.0 8.978498292289625E-5 0.0 0.5234240041947544 1.122312286536203E-4 40-41 0.0 8.978498292289625E-5 0.0 0.7430605186698893 1.3467747438434437E-4 42-43 0.0 8.978498292289625E-5 0.0 0.9385673189844959 1.3467747438434437E-4 44-45 0.0 8.978498292289625E-5 0.0 1.1692922788506086 1.5712372011506844E-4 46-47 0.0 8.978498292289625E-5 0.0 1.4419468257417138 1.795699658457925E-4 48-49 0.0 8.978498292289625E-5 0.0 1.7278895501054077 2.244624573072406E-4 50-51 0.0 8.978498292289625E-5 0.0 2.0301506951153376 2.244624573072406E-4 52-53 0.0 8.978498292289625E-5 0.0 2.327698128521816 2.244624573072406E-4 54-55 0.0 8.978498292289625E-5 0.0 2.6675791813764396 2.244624573072406E-4 56-57 0.0 8.978498292289625E-5 0.0 3.039333903168692 2.244624573072406E-4 58-59 0.0 8.978498292289625E-5 0.0 3.4467332631813337 2.4690870303796465E-4 60-61 0.0 8.978498292289625E-5 0.0 3.8574995600535837 2.6935494876868875E-4 62-63 0.0 8.978498292289625E-5 0.0 4.2783217750131985 2.6935494876868875E-4 64-65 0.0 8.978498292289625E-5 0.0 4.692275438779212 2.6935494876868875E-4 66-67 0.0 8.978498292289625E-5 0.0 5.16158154451719 2.6935494876868875E-4 68-69 0.0 8.978498292289625E-5 0.0 5.687609313216709 2.6935494876868875E-4 70-71 0.0 8.978498292289625E-5 0.0 6.212716785841268 2.6935494876868875E-4 72-73 0.0 8.978498292289625E-5 0.0 6.700473705569902 2.6935494876868875E-4 74-75 0.0 8.978498292289625E-5 0.0 7.1888591201789955 2.6935494876868875E-4 76-77 0.0 8.978498292289625E-5 0.0 7.739555312936579 2.6935494876868875E-4 78-79 0.0 8.978498292289625E-5 0.0 8.329420204494276 2.6935494876868875E-4 80-81 0.0 8.978498292289625E-5 0.0 8.909363855438993 2.9180119449941284E-4 82-83 0.0 8.978498292289625E-5 0.0 9.494919067816394 3.142474402301369E-4 84-85 0.0 8.978498292289625E-5 0.0 10.071742690604541 3.142474402301369E-4 86-87 0.0 1.3467747438434437E-4 0.0 10.677454631648128 3.142474402301369E-4 88 0.0 1.3467747438434437E-4 0.0 11.136435464349974 3.142474402301369E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4620 0.0 60.641815 1 TACCTGG 2365 0.0 48.99476 2 ACCTGGG 2750 0.0 41.96867 3 GTATCAA 8345 0.0 40.32113 1 GTACCTG 3250 0.0 36.09911 1 CCTGGGG 2550 0.0 34.037243 4 TATCACG 155 4.1836756E-11 33.292377 2 ATCAACG 10740 0.0 30.928 3 CAACGCA 10875 0.0 30.449574 5 TATCAAC 11025 0.0 30.168344 2 TCAACGC 11020 0.0 30.14217 4 AACGCAG 11405 0.0 29.15859 6 GTACATG 8145 0.0 27.886507 1 TACATGG 8285 0.0 26.95245 2 CTGGGGG 1885 0.0 26.624302 5 TAACGCA 250 0.0 26.273071 4 ACATGGG 8390 0.0 26.226099 3 CATGGGG 5640 0.0 25.704086 4 TGGGGGG 4110 0.0 25.677708 6 ACGCAGA 12875 0.0 25.574398 7 >>END_MODULE