FastQCFastQC Report
Mon 6 Jun 2016
ERR1211350_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211350_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4887669
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301080.6159991603359393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225910.4622039667579781No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162380.3322238064811672No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152800.3126234612041036No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG73100.14956004590327207No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72550.14843476512014214No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71160.1455908736864137No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT66130.13529966943342522No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC52570.10755638321662125No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT51100.10454881457807393No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49670.10162308454193605No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG63700.070.150822
ACCTGGG68350.064.014983
GTACCTG83300.053.759331
GGTATCA121350.047.834071
CCTGGGG43200.045.6910974
TATCACG4000.041.1582222
GTATCAA224900.039.5515941
TAACGCA6500.036.8742184
ATCAACG270150.032.1493
CAACGCA272800.031.8350735
GTACACG6450.031.359611
TCAACGC277900.031.3197614
AACGCAG288950.030.4629676
TATCAAC292200.029.7810552
GTACATG265400.028.7660031
CTGGGGG35750.028.2625055
TATAACG8500.028.2227822
ACGCAGA314650.027.6913157
CGCAGAG318850.027.3245988
CATGGGT28400.027.1388574