##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211350_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4887669 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.546799711682603 31.0 31.0 34.0 30.0 34.0 2 31.544534828360923 33.0 31.0 34.0 29.0 34.0 3 31.58829004173564 33.0 31.0 34.0 28.0 34.0 4 35.2202469111554 37.0 35.0 37.0 32.0 37.0 5 35.2847987455779 37.0 35.0 37.0 33.0 37.0 6 35.356813032961114 37.0 35.0 37.0 33.0 37.0 7 35.316553760084815 37.0 35.0 37.0 32.0 37.0 8 35.31509294103181 37.0 35.0 37.0 32.0 37.0 9 36.898348476543724 39.0 37.0 39.0 33.0 39.0 10-11 36.815894140949396 39.0 37.0 39.0 32.5 39.0 12-13 36.75993628046416 39.0 37.0 39.0 32.0 39.0 14-15 37.89693236182728 40.0 38.0 41.0 32.0 41.0 16-17 37.90720586848251 40.0 38.0 41.0 32.5 41.0 18-19 37.962015737972436 40.0 38.0 41.0 33.0 41.0 20-21 37.9327291803107 40.0 38.0 41.0 32.5 41.0 22-23 37.78589108632356 40.0 37.5 41.0 32.0 41.0 24-25 37.686651141883786 40.0 37.0 41.0 32.0 41.0 26-27 37.543475018459716 40.0 37.0 41.0 32.0 41.0 28-29 37.41119437506918 40.0 37.0 41.0 32.0 41.0 30-31 37.20935654603452 39.5 36.0 41.0 31.0 41.0 32-33 37.040588673250994 39.0 36.0 41.0 31.0 41.0 34-35 36.873784517732275 39.0 35.5 41.0 30.0 41.0 36-37 36.75449554787773 39.0 35.0 41.0 30.0 41.0 38-39 36.61296008383546 39.0 35.0 41.0 30.0 41.0 40-41 36.4613165294131 39.0 35.0 41.0 30.0 41.0 42-43 36.32183726025637 39.0 35.0 41.0 29.0 41.0 44-45 36.18092284890814 39.0 35.0 41.0 28.5 41.0 46-47 35.983492130911486 39.0 35.0 41.0 27.5 41.0 48-49 35.735729649450484 39.0 35.0 41.0 26.0 41.0 50-51 35.08182397375927 38.5 34.0 39.5 25.0 40.5 52-53 35.32888397311684 38.5 34.5 40.0 26.0 40.5 54-55 35.71469242291162 39.0 35.0 40.5 26.0 41.0 56-57 35.656280836529646 39.0 35.0 41.0 26.0 41.0 58-59 35.48264571516606 39.0 35.0 40.5 26.0 41.0 60-61 35.26082289942302 38.0 34.0 40.0 26.0 41.0 62-63 35.012939501426956 38.0 34.0 40.0 25.5 41.0 64-65 34.77878146003749 38.0 34.0 40.0 25.0 41.0 66-67 34.569292130870565 37.0 34.0 40.0 25.0 41.0 68-69 34.23838469830915 37.0 33.5 39.5 24.0 41.0 70-71 33.904738127725096 36.0 33.0 39.0 23.5 41.0 72-73 33.53325450229956 36.0 33.0 39.0 23.0 40.5 74-75 33.13377593695482 35.0 32.5 38.5 22.0 40.0 76-77 32.74969827130274 35.0 32.0 37.0 21.5 39.5 78-79 32.352745756719614 35.0 32.0 37.0 20.5 39.0 80-81 31.991860639499116 35.0 31.0 36.0 20.0 39.0 82-83 31.645139124601116 35.0 31.0 36.0 20.0 37.5 84-85 31.286459455417294 35.0 31.0 35.5 19.0 37.0 86-87 31.025436051418374 34.5 31.0 35.0 18.5 37.0 88-89 30.733393157351692 34.0 30.0 35.0 18.0 36.0 90-91 30.454422036353115 34.0 30.0 35.0 17.5 36.0 92-93 30.199172959543702 34.0 29.0 35.0 16.0 36.0 94-95 29.961267426251656 34.0 29.0 35.0 15.0 35.0 96-97 29.75102998996045 34.0 29.0 35.0 12.5 35.0 98-99 29.516725150577912 34.0 29.0 35.0 10.5 35.0 100 27.904461820143712 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2549623152898697 1101 2 -0.23293936265453752 1101 3 -0.2594510309075666 1101 4 -0.1806570909150622 1101 5 -0.22782503871705728 1101 6 -0.25756189090682113 1101 7 -0.26761368429492194 1101 8 -0.40216182074507856 1101 9 -0.4152043280120381 1101 10-11 -0.46813043204512894 1101 12-13 -0.3804102836503631 1101 14-15 -0.4149090474238193 1101 16-17 -0.42166356429662954 1101 18-19 -0.464002314621645 1101 20-21 -0.4726049290831327 1101 22-23 -0.5640193161546918 1101 24-25 -0.5349350535845048 1101 26-27 -0.6709059541742661 1101 28-29 -0.6045820173116638 1101 30-31 -0.5963391660003978 1101 32-33 -0.5986851527976924 1101 34-35 -0.5064448869794802 1101 36-37 -0.5294416729494884 1101 38-39 -0.4992888350708071 1101 40-41 -0.63324766257211 1101 42-43 -0.6386749293078182 1101 44-45 -0.763658337111508 1101 46-47 -0.6947953099064179 1101 48-49 -0.6771269580546644 1101 50-51 -0.6287989666706153 1101 52-53 -0.40770237835865686 1101 54-55 -0.43845309791439746 1101 56-57 -0.3599908455239742 1101 58-59 -0.4945397383895269 1101 60-61 -0.391937484126494 1101 62-63 -0.4479558201775973 1101 64-65 -0.4723827491902668 1101 66-67 -0.41213329706224755 1101 68-69 -0.4308306161841742 1101 70-71 -0.4687772132566792 1101 72-73 -0.5330616815826659 1101 74-75 -0.4463103675882536 1101 76-77 -0.5037495457335197 1101 78-79 -0.6312409447026681 1101 80-81 -0.648435200607814 1101 82-83 -0.6712319704430847 1101 84-85 -0.669498822944103 1101 86-87 -0.5893009237043962 1101 88-89 -0.7407302191238152 1101 90-91 -0.850821571282637 1101 92-93 -0.7028047998944942 1101 94-95 -0.7948659238738145 1101 96-97 -0.89827297228668 1101 98-99 -0.8168861761833419 1101 100 -0.7928653121149125 1102 1 -0.319294203617531 1102 2 -0.345325697821 1102 3 -0.3545998895342244 1102 4 -0.2542069839681247 1102 5 -0.17076511247108783 1102 6 -0.1894952010113755 1102 7 -0.24253574669055666 1102 8 -0.2533552890104289 1102 9 -0.34314026414035936 1102 10-11 -0.34087234429549795 1102 12-13 -0.44598395916062117 1102 14-15 -0.4220233012346952 1102 16-17 -0.4256376129324124 1102 18-19 -0.49524398592674146 1102 20-21 -0.5063621659323303 1102 22-23 -0.5152977495948576 1102 24-25 -0.5145735297874552 1102 26-27 -0.523064427236541 1102 28-29 -0.6162524043784572 1102 30-31 -0.5280709641433958 1102 32-33 -0.5373132851361788 1102 34-35 -0.45856887649775757 1102 36-37 -0.5946593498215051 1102 38-39 -0.728090046430161 1102 40-41 -0.6731950551886356 1102 42-43 -0.653629244605682 1102 44-45 -0.6376906101924078 1102 46-47 -0.6323758747332917 1102 48-49 -0.5937132180605857 1102 50-51 -0.5897830892562155 1102 52-53 -0.5486119837238661 1102 54-55 -0.41314777570104155 1102 56-57 -0.40801604827962024 1102 58-59 -0.37474790731333485 1102 60-61 -0.37693092406179574 1102 62-63 -0.3064997425428757 1102 64-65 -0.4318469786069272 1102 66-67 -0.34515083592015827 1102 68-69 -0.4299795159673252 1102 70-71 -0.4379338458646842 1102 72-73 -0.44591743547964313 1102 74-75 -0.4729945320895368 1102 76-77 -0.460376474744173 1102 78-79 -0.39659316433607117 1102 80-81 -0.48867055379242785 1102 82-83 -0.5362671302721118 1102 84-85 -0.5370471508518406 1102 86-87 -0.5618248713869072 1102 88-89 -0.6980961918174806 1102 90-91 -0.7149296112627717 1102 92-93 -0.659165635219388 1102 94-95 -0.6017868720474056 1102 96-97 -0.7110143421104986 1102 98-99 -0.7382482122388225 1102 100 -0.7966128002704345 1103 1 -0.5014735154138066 1103 2 -0.48131345607277964 1103 3 -0.41809299697398217 1103 4 -0.3150518049018629 1103 5 -0.31238267183830715 1103 6 -0.3598931455201324 1103 7 -0.4268240416149638 1103 8 -0.4347146842891121 1103 9 -0.6030011852529924 1103 10-11 -0.610609532551841 1103 12-13 -0.6805853131190176 1103 14-15 -0.669065901816964 1103 16-17 -0.8322767444347434 1103 18-19 -0.6752134692477938 1103 20-21 -0.8694163768832581 1103 22-23 -0.7696582147111357 1103 24-25 -0.8094146325970968 1103 26-27 -0.7135459418459646 1103 28-29 -0.8078782944482654 1103 30-31 -0.750152149032381 1103 32-33 -0.87180853225113 1103 34-35 -0.9135019678022616 1103 36-37 -0.9099917887004523 1103 38-39 -0.9623581722851569 1103 40-41 -0.8157224149697626 1103 42-43 -0.8795863984869143 1103 44-45 -0.9641226141209955 1103 46-47 -0.8995963138928502 1103 48-49 -0.8730964829944696 1103 50-51 -0.8920187867552514 1103 52-53 -0.8508827578653566 1103 54-55 -0.744834699128738 1103 56-57 -0.680576906254295 1103 58-59 -0.8104984597704572 1103 60-61 -0.7016069035770371 1103 62-63 -0.729039554532072 1103 64-65 -0.6752503775335796 1103 66-67 -0.730890865384545 1103 68-69 -0.7051645452563235 1103 70-71 -0.6563845982586685 1103 72-73 -0.816416663793504 1103 74-75 -0.7281924520446381 1103 76-77 -0.7541937780518992 1103 78-79 -0.8099056270671419 1103 80-81 -0.8145983309855929 1103 82-83 -0.7932013440291747 1103 84-85 -0.8196596591825447 1103 86-87 -0.866412896221167 1103 88-89 -0.9173602838585921 1103 90-91 -0.9877798515377734 1103 92-93 -0.9529851308172717 1103 94-95 -1.1164160242449555 1103 96-97 -1.0577907986680763 1103 98-99 -1.0026647122914412 1103 100 -1.1078697717531263 1104 1 -0.3969273967959914 1104 2 -0.38176800353966556 1104 3 -0.45372229005084463 1104 4 -0.2975886136276955 1104 5 -0.2783307518441305 1104 6 -0.2633731462767699 1104 7 -0.31166353130151947 1104 8 -0.2311757018331022 1104 9 -0.2819244140613364 1104 10-11 -0.4023934180512754 1104 12-13 -0.3915090820395406 1104 14-15 -0.5471984619286019 1104 16-17 -0.5037484273832504 1104 18-19 -0.6234594428563511 1104 20-21 -0.5299557686493301 1104 22-23 -0.5038276352657505 1104 24-25 -0.6152801724335859 1104 26-27 -0.6279051583049764 1104 28-29 -0.6107405167402433 1104 30-31 -0.6441655971013205 1104 32-33 -0.661903359771415 1104 34-35 -0.6190037368507788 1104 36-37 -0.5879836057191383 1104 38-39 -0.6577863661411456 1104 40-41 -0.7448778175606137 1104 42-43 -0.6101813992697132 1104 44-45 -0.6904245380392311 1104 46-47 -0.6834398057221449 1104 48-49 -0.6364801570371625 1104 50-51 -0.6338230270786127 1104 52-53 -0.5106094156213885 1104 54-55 -0.43598606576951937 1104 56-57 -0.491840851270716 1104 58-59 -0.55638875743184 1104 60-61 -0.46766623582611544 1104 62-63 -0.5106229364634274 1104 64-65 -0.4517595423358145 1104 66-67 -0.5646048832275667 1104 68-69 -0.5697024877347729 1104 70-71 -0.5729799777639428 1104 72-73 -0.5912699239259425 1104 74-75 -0.6262204690561646 1104 76-77 -0.5208740179550873 1104 78-79 -0.4890947866694866 1104 80-81 -0.5397949768969816 1104 82-83 -0.6294602179475888 1104 84-85 -0.7504923475607548 1104 86-87 -0.7297691420631693 1104 88-89 -0.63641712804991 1104 90-91 -0.5049030578883986 1104 92-93 -0.5479499430316963 1104 94-95 -0.6044300715888866 1104 96-97 -0.7157104249845005 1104 98-99 -0.7582715959447555 1104 100 -0.8786229384727164 1105 1 -0.38085643624262033 1105 2 -0.36111488992684926 1105 3 -0.37207886118864764 1105 4 -0.1789203361456373 1105 5 -0.2885095511725666 1105 6 -0.258440966271003 1105 7 -0.24096108654934767 1105 8 -0.23324017757325066 1105 9 -0.41664099201781113 1105 10-11 -0.39703121266425256 1105 12-13 -0.3905931429695002 1105 14-15 -0.4030454111348263 1105 16-17 -0.5392504056425551 1105 18-19 -0.44662414358503355 1105 20-21 -0.5032109278233534 1105 22-23 -0.45433315655864703 1105 24-25 -0.4917931873801962 1105 26-27 -0.5088327842738778 1105 28-29 -0.5698624961910426 1105 30-31 -0.5021553738174518 1105 32-33 -0.503495128569007 1105 34-35 -0.6177934379481158 1105 36-37 -0.6303620823208576 1105 38-39 -0.582875905403256 1105 40-41 -0.6511252799758438 1105 42-43 -0.7143038722285198 1105 44-45 -0.7454775439453982 1105 46-47 -0.7082137357977629 1105 48-49 -0.7464214531779518 1105 50-51 -0.6916747985069378 1105 52-53 -0.4813146277541307 1105 54-55 -0.5275145731319313 1105 56-57 -0.6103706859927129 1105 58-59 -0.5032948652717906 1105 60-61 -0.5896123355104095 1105 62-63 -0.6331239623112523 1105 64-65 -0.48620817100289315 1105 66-67 -0.5290433792510854 1105 68-69 -0.48688646122926826 1105 70-71 -0.5643743826807821 1105 72-73 -0.5312353872129805 1105 74-75 -0.5743130851735856 1105 76-77 -0.543806239053751 1105 78-79 -0.5976093334570933 1105 80-81 -0.5647911821939537 1105 82-83 -0.5950347409013155 1105 84-85 -0.5224231504628953 1105 86-87 -0.49785404785524534 1105 88-89 -0.5702429142170189 1105 90-91 -0.6104565074602348 1105 92-93 -0.7280154095235822 1105 94-95 -0.7215932233709133 1105 96-97 -0.7110173309311172 1105 98-99 -0.6737820415038414 1105 100 -0.6486414224121759 1106 1 -0.42444808966534353 1106 2 -0.4143583105481241 1106 3 -0.5163600094689258 1106 4 -0.35064665460983946 1106 5 -0.31190291413960125 1106 6 -0.32779816094637937 1106 7 -0.3670936543664354 1106 8 -0.33115568763412995 1106 9 -0.469435534414103 1106 10-11 -0.5072386129967796 1106 12-13 -0.5147108004154077 1106 14-15 -0.589279679431101 1106 16-17 -0.5977889268067074 1106 18-19 -0.5535998764687449 1106 20-21 -0.6234531125293188 1106 22-23 -0.6003808959334407 1106 24-25 -0.585893330902195 1106 26-27 -0.6735846929777765 1106 28-29 -0.7258282752025806 1106 30-31 -0.7334714571301575 1106 32-33 -0.7128012752740744 1106 34-35 -0.6741763500601081 1106 36-37 -0.6600012026238105 1106 38-39 -0.6777234169056996 1106 40-41 -0.6721292492968942 1106 42-43 -0.6324909462375032 1106 44-45 -0.6408956561080004 1106 46-47 -0.6966396522734541 1106 48-49 -0.6936367727238135 1106 50-51 -0.7813893043810864 1106 52-53 -0.5879327484162005 1106 54-55 -0.6231782402761894 1106 56-57 -0.581630379654726 1106 58-59 -0.5765610042663454 1106 60-61 -0.5671940751481515 1106 62-63 -0.567176933778363 1106 64-65 -0.6374898472044848 1106 66-67 -0.7798025151600143 1106 68-69 -0.5622246655914367 1106 70-71 -0.6044778105984179 1106 72-73 -0.6578076107405622 1106 74-75 -0.6081455475490429 1106 76-77 -0.6243124700058758 1106 78-79 -0.6484161903449674 1106 80-81 -0.7606590923736469 1106 82-83 -0.6528951982071973 1106 84-85 -0.6065419510087295 1106 86-87 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1.2161338187564041 2311 1 0.5288496556672477 2311 2 0.43084333631285077 2311 3 0.5048769565184088 2311 4 0.4142335305435907 2311 5 0.39060572923611403 2311 6 0.2633335027592665 2311 7 0.36212050942965135 2311 8 0.4174363721247687 2311 9 0.5035845278217721 2311 10-11 0.5408545474328008 2311 12-13 0.574526385884532 2311 14-15 0.7261471583742178 2311 16-17 0.7155690329753739 2311 18-19 0.6031627548812821 2311 20-21 0.691414365714003 2311 22-23 0.7260720964485756 2311 24-25 0.691153771378815 2311 26-27 0.7646948068055579 2311 28-29 0.7839448717746436 2311 30-31 0.6770572136013158 2311 32-33 0.7483766605656399 2311 34-35 0.7842910148795923 2311 36-37 0.823107448636442 2311 38-39 0.7763889618230095 2311 40-41 0.7526620864479767 2311 42-43 0.7422508597319961 2311 44-45 0.8618978159227666 2311 46-47 0.826618961616397 2311 48-49 0.7470876209257966 2311 50-51 0.75029006081364 2311 52-53 0.697601782987185 2311 54-55 0.7409391611903047 2311 56-57 0.6259327180831136 2311 58-59 0.6487861503024064 2311 60-61 0.660644128613086 2311 62-63 0.583507917167509 2311 64-65 0.5869905362951684 2311 66-67 0.5000613193271519 2311 68-69 0.5849475541260887 2311 70-71 0.5811750942101526 2311 72-73 0.510888747888103 2311 74-75 0.5528131221003321 2311 76-77 0.5228834763072854 2311 78-79 0.5888364629373584 2311 80-81 0.5801073034339908 2311 82-83 0.5608696463272302 2311 84-85 0.6125294784549808 2311 86-87 0.6425653486317877 2311 88-89 0.67245453840167 2311 90-91 0.72212283294391 2311 92-93 0.734070620348767 2311 94-95 0.6330479036292189 2311 96-97 0.6651467727174634 2311 98-99 0.7925223986584022 2311 100 0.8890497400325863 2312 1 0.4498560453163023 2312 2 0.39085795701144477 2312 3 0.4727799541335145 2312 4 0.3692508421520131 2312 5 0.3999244323078983 2312 6 0.398406284830628 2312 7 0.41761023111041595 2312 8 0.42508819867610725 2312 9 0.5316369307006497 2312 10-11 0.5996183864924873 2312 12-13 0.5419135815102223 2312 14-15 0.7182169435211421 2312 16-17 0.7154261863062388 2312 18-19 0.707706129000627 2312 20-21 0.7161258433342965 2312 22-23 0.7199140834610205 2312 24-25 0.7449870428185648 2312 26-27 0.7903048039207405 2312 28-29 0.7903174536083171 2312 30-31 0.7896280923786136 2312 32-33 0.8548572639669629 2312 34-35 0.8137972528495254 2312 36-37 0.8598168510950686 2312 38-39 0.9174668823899026 2312 40-41 0.8737190933593411 2312 42-43 0.9461537548109433 2312 44-45 0.8360995480930313 2312 46-47 0.879864908118023 2312 48-49 0.8245834995570007 2312 50-51 0.8336793772906717 2312 52-53 0.8077127239130846 2312 54-55 0.7707118337658088 2312 56-57 0.6838806509702806 2312 58-59 0.6932560915380392 2312 60-61 0.7283499300871625 2312 62-63 0.7077160512061695 2312 64-65 0.738168798844022 2312 66-67 0.7878640865281241 2312 68-69 0.7895071719903299 2312 70-71 0.7937761627112039 2312 72-73 0.7823552618662291 2312 74-75 0.6982963117502408 2312 76-77 0.7182347635609787 2312 78-79 0.7609737386528153 2312 80-81 0.7708267990623909 2312 82-83 0.7916668967645997 2312 84-85 0.8101942043726673 2312 86-87 0.7263745718912311 2312 88-89 0.7642178839065217 2312 90-91 0.7915200160155642 2312 92-93 0.8230327499767753 2312 94-95 0.762012479780374 2312 96-97 0.8045930137581685 2312 98-99 0.9016326823966487 2312 100 0.8642387810964571 2313 1 0.44645469000023397 2313 2 0.39930492183227884 2313 3 0.4248590725184087 2313 4 0.297318540068062 2313 5 0.33972555398853643 2313 6 0.29742591904516047 2313 7 0.3823954856917027 2313 8 0.3587748671744535 2313 9 0.40633910636657333 2313 10-11 0.46644314774948015 2313 12-13 0.46983655418806336 2313 14-15 0.666680092463082 2313 16-17 0.5775439589305407 2313 18-19 0.6168124510550328 2313 20-21 0.6094159816518498 2313 22-23 0.5122016324472867 2313 24-25 0.5779873271774818 2313 26-27 0.6473737167666513 2313 28-29 0.6261491105689885 2313 30-31 0.6979911905330312 2313 32-33 0.6490232309441879 2313 34-35 0.6964577154963578 2313 36-37 0.7369438269572228 2313 38-39 0.7879016729345807 2313 40-41 0.7754168569789499 2313 42-43 0.5617968254517294 2313 44-45 0.6709838543629516 2313 46-47 0.7359864062283847 2313 48-49 0.7681997219769059 2313 50-51 0.6869148885955383 2313 52-53 0.5678185451371505 2313 54-55 0.5063232246459606 2313 56-57 0.5091623680370034 2313 58-59 0.5716182034025294 2313 60-61 0.6253448839973288 2313 62-63 0.5966158543964966 2313 64-65 0.6023620392995852 2313 66-67 0.5838021731384657 2313 68-69 0.6277859167378566 2313 70-71 0.6232143713269025 2313 72-73 0.5909350556035449 2313 74-75 0.5601303659078027 2313 76-77 0.6112588437655617 2313 78-79 0.7252050217199439 2313 80-81 0.6469929428032586 2313 82-83 0.6333097547766293 2313 84-85 0.6545653388686681 2313 86-87 0.6781905141791853 2313 88-89 0.6277500539300966 2313 90-91 0.6086494223779013 2313 92-93 0.6811181658869145 2313 94-95 0.7250618320632825 2313 96-97 0.8115851689105398 2313 98-99 0.8604791907061404 2313 100 1.0122096176560476 2314 1 0.33320255251926767 2314 2 0.19554373776531975 2314 3 0.3010739080758711 2314 4 0.20902702110106475 2314 5 0.2144850014190638 2314 6 0.26383221250308964 2314 7 0.26410059247864126 2314 8 0.33933416679833783 2314 9 0.39173904316058383 2314 10-11 0.3744166990395499 2314 12-13 0.4518286399917244 2314 14-15 0.46553987847964606 2314 16-17 0.4859864051122358 2314 18-19 0.4237865958945406 2314 20-21 0.43508510014839885 2314 22-23 0.4893977501082247 2314 24-25 0.5494565026450005 2314 26-27 0.5250809379588759 2314 28-29 0.5480947149105191 2314 30-31 0.5773278149788368 2314 32-33 0.6000334569881147 2314 34-35 0.5958703857903416 2314 36-37 0.6195715027435327 2314 38-39 0.6491067372378083 2314 40-41 0.6117458423028168 2314 42-43 0.5028552873212178 2314 44-45 0.6310724069275295 2314 46-47 0.3849740865625719 2314 48-49 0.33004635149018924 2314 50-51 0.5457936486334631 2314 52-53 0.5326664656663169 2314 54-55 0.4684770664268356 2314 56-57 0.5705965998856115 2314 58-59 0.5345747734324959 2314 60-61 0.31630216433054414 2314 62-63 0.605501980315772 2314 64-65 0.5745289410020575 2314 66-67 0.5820143270260942 2314 68-69 0.5312890351647255 2314 70-71 0.5385485317172822 2314 72-73 0.5818016225098859 2314 74-75 0.576407328548413 2314 76-77 0.5632963963124169 2314 78-79 0.5891907826460567 2314 80-81 0.6262151362954853 2314 82-83 0.6244620475829485 2314 84-85 0.6333930145327145 2314 86-87 0.6013352748369414 2314 88-89 0.5659223281413794 2314 90-91 0.5544418231111905 2314 92-93 0.7500392201432007 2314 94-95 0.7987187354223124 2314 96-97 0.7586882533504387 2314 98-99 0.7962995481683883 2314 100 0.8773145952191435 2315 1 0.34503047942433085 2315 2 0.31469262016806 2315 3 0.37455647700023675 2315 4 0.29234110055820395 2315 5 0.2893026461525565 2315 6 0.2744014774201844 2315 7 0.20956645505530958 2315 8 0.26273042420581305 2315 9 0.17271961299108796 2315 10-11 0.32914957256625144 2315 12-13 0.31035902471425203 2315 14-15 0.41425218525647267 2315 16-17 0.3099705224694205 2315 18-19 0.4503008982152963 2315 20-21 0.37278837144214094 2315 22-23 0.3198300357298507 2315 24-25 0.44552277027508325 2315 26-27 0.46885641550012735 2315 28-29 0.4889483565706101 2315 30-31 0.5471094278880813 2315 32-33 0.5770121387353768 2315 34-35 0.36950242797076527 2315 36-37 0.570821319982123 2315 38-39 0.5477508732592256 2315 40-41 0.5730324645291276 2315 42-43 0.5028116652579584 2315 44-45 0.5128293742735579 2315 46-47 0.5066428651387938 2315 48-49 0.466882927597446 2315 50-51 0.44628083186959344 2315 52-53 0.163871328112414 2315 54-55 0.33570298298654677 2315 56-57 0.2931402114193986 2315 58-59 0.29930478595353094 2315 60-61 0.41976101789458653 2315 62-63 0.4479393285034732 2315 64-65 0.38619910645903843 2315 66-67 0.2976723763797935 2315 68-69 0.3794361955489123 2315 70-71 0.3075130732975353 2315 72-73 0.3454384661256995 2315 74-75 0.11390348529423022 2315 76-77 0.30224236993581144 2315 78-79 0.3221033887003131 2315 80-81 0.33557122650711335 2315 82-83 0.2658226528285468 2315 84-85 0.3108869480575045 2315 86-87 0.31693741273921816 2315 88-89 0.13942714431800596 2315 90-91 0.3936343215603486 2315 92-93 0.48507086569377833 2315 94-95 0.46466342685788575 2315 96-97 0.5116796889856339 2315 98-99 0.6598681307762426 2315 100 0.7630729241752299 2316 1 -0.020040708335489654 2316 2 0.0076305698857410675 2316 3 0.02383404752382745 2316 4 -0.10424218631443694 2316 5 -0.03630032633397917 2316 6 -0.16267542781123012 2316 7 -0.09131249394769014 2316 8 -0.10073238790714356 2316 9 -0.0679296642800935 2316 10-11 -0.0034722698216143044 2316 12-13 -0.03552091586595196 2316 14-15 -0.25372301408796716 2316 16-17 -0.11680236144869127 2316 18-19 0.0018103032315508472 2316 20-21 0.07497500498139686 2316 22-23 -0.08505196658258285 2316 24-25 -0.016239019203723615 2316 26-27 -0.13075391336673903 2316 28-29 -0.15568653721836512 2316 30-31 -0.7020283489584074 2316 32-33 -0.49122865362835455 2316 34-35 -0.7301821382235616 2316 36-37 -0.40467422540404385 2316 38-39 -0.3400004167265891 2316 40-41 -0.11734691665186148 2316 42-43 -0.08765549856930477 2316 44-45 -0.24558814334096724 2316 46-47 -0.2998196738227108 2316 48-49 -0.30529446369388324 2316 50-51 -0.14253964027082588 2316 52-53 -0.39652043842915674 2316 54-55 -0.41711268468167617 2316 56-57 -0.3872795437919905 2316 58-59 -0.7841646117287908 2316 60-61 -0.3060794841054033 2316 62-63 -0.40026307298426644 2316 64-65 -0.6361596499160598 2316 66-67 -0.966997615500496 2316 68-69 -0.9420117316805445 2316 70-71 -0.8110506738914367 2316 72-73 -0.9636307609305135 2316 74-75 -0.6696073715407351 2316 76-77 -0.366924667792766 2316 78-79 -0.3263101251873799 2316 80-81 -0.4048537672253687 2316 82-83 -0.21511549146277176 2316 84-85 -0.15135588732945493 2316 86-87 -0.011006579986720055 2316 88-89 0.07374768942354493 2316 90-91 -0.6373116430945984 2316 92-93 -0.5933303376693502 2316 94-95 -0.1775595769849474 2316 96-97 -0.017413261087522613 2316 98-99 0.1572158675480395 2316 100 0.02137736457021333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 139.0 1 350.0 2 217.0 3 202.0 4 199.0 5 156.0 6 241.0 7 861.0 8 2423.0 9 3454.0 10 4256.0 11 5781.0 12 7546.0 13 9130.0 14 10868.0 15 12938.0 16 16209.0 17 22703.0 18 29494.0 19 34927.0 20 41284.0 21 50538.0 22 55932.0 23 54073.0 24 49682.0 25 49288.0 26 52628.0 27 59439.0 28 68700.0 29 81495.0 30 100217.0 31 122926.0 32 156425.0 33 204990.0 34 287369.0 35 410584.0 36 595898.0 37 941772.0 38 1069944.0 39 272199.0 40 192.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.43684059516856 20.531784658442774 13.27818314207515 21.75319160431351 2 15.312768317701266 21.831126983502763 42.20238036717331 20.653724331622655 3 17.062681086686897 31.430192700074507 30.181977348445827 21.325148864792766 4 11.27536093682631 18.322614997103422 40.97788387772195 29.424140188348318 5 11.121690860937886 38.964870728553905 35.67448667749608 14.238951733012135 6 25.625903197677992 39.82666792210291 21.27248015523742 13.27494872498168 7 25.721638859509806 33.228189536397736 25.386168057178025 15.664003546914431 8 25.25356310556011 34.88355766526682 22.669137244804602 17.19374198436847 9 27.31521002560966 16.758680381093345 22.828761164188556 33.097348429108436 10-11 22.90935605188426 27.68535830327219 30.488397676977 18.916887967866554 12-13 23.970755026786776 26.279562238232696 30.010369636133532 19.739313098846996 14-15 21.929208962435894 27.186552866923027 28.12614162505395 22.75809654558713 16-17 19.765135118788915 29.746396237036876 29.442445864628336 21.046022779545872 18-19 19.594980912810076 28.80848103054759 31.811396479459077 19.785141577183257 20-21 20.441414051989405 28.438552419006115 31.702359465521468 19.417674063483016 22-23 20.671277191534514 27.973854041803403 31.32744592806566 20.027422838596422 24-25 20.18834168761678 28.386929421821893 31.739179704122527 19.6855491864388 26-27 19.73723766680461 28.511895433881612 31.839435620065494 19.911431279248287 28-29 19.691701781194443 27.684416518856423 32.517345740864506 20.106535959084624 30-31 20.353339769689267 28.333155417526097 31.82147883550744 19.4920259772772 32-33 20.20719497205503 28.60685560009784 31.664140481159027 19.521808946688104 34-35 20.281464231350594 28.477080715636017 31.740251829091747 19.501203223921646 36-37 20.437296668705628 28.254050353755133 31.14583340795658 20.16281956958266 38-39 19.95519300118363 28.987784514975495 31.40915350286236 19.647868980978515 40-41 19.934308269041313 28.147330441791734 32.25259279161709 19.665768497549866 42-43 19.91399669411743 28.26084353128198 31.20998598034751 20.615173794253078 44-45 19.545168449893303 28.815391388451957 31.30193542879481 20.33750473285993 46-47 19.89371685561084 28.358986285306248 31.02310731002676 20.724189549056153 48-49 19.901341295306988 28.757368515708738 30.791446835677426 20.549843353306848 50-51 20.084339470462762 28.48868581909593 30.556865683252067 20.870109027189244 52-53 19.943470693079764 28.9358007888553 30.355758754242462 20.76496976382247 54-55 20.15984899613586 28.953396328031783 30.28701337700835 20.59974129882401 56-57 20.176145747781135 29.11169490179188 29.92465401770178 20.787505332725203 58-59 19.752912924543693 28.681951157401198 30.670318087532156 20.89481783052295 60-61 20.4434456496441 29.649692409002583 29.812274613574292 20.094587327779028 62-63 20.050805689221814 29.20064050624723 30.622301114089456 20.126252690441497 64-65 19.959614995471323 28.583465464505853 30.403881775191984 21.05303776483084 66-67 20.06869079612727 28.781435389127243 29.719219513213652 21.43065430153184 68-69 19.702095841327957 29.08342575452284 30.157176584266377 21.05730181988283 70-71 20.242085628676136 28.32963188568295 30.55368381984075 20.87459866580017 72-73 20.070121183826227 28.941284154059836 29.756362051075335 21.2322326110386 74-75 19.943659826783087 29.103231602163827 29.85187854652875 21.10123002452434 76-77 19.876559169235964 28.574898310544107 30.585934537383984 20.962607982835948 78-79 19.679181246514553 28.544714854616434 30.095201322688713 21.6809025761803 80-81 19.632249434343287 29.22672290887174 29.56238069618154 21.578646960603432 82-83 20.52605888480241 28.598914593116735 29.743911077821544 21.13111544425931 84-85 19.75959085304969 28.917104547322587 29.435897803337806 21.88740679628992 86-87 19.50432142974487 29.082358807995945 30.262669682354776 21.150650079904405 88-89 20.08000572419982 28.49923702926722 30.094601819562616 21.326155426970335 90-91 20.25884674211953 28.579033203006034 30.11535158529733 21.04676846957711 92-93 20.327512588999323 28.67684365316647 29.831331774237075 21.164311983597138 94-95 19.945987666170236 27.92050057694853 30.139830292874453 21.993681464006777 96-97 20.5074608071523 28.073007444171278 29.863529498482038 21.556002250194382 98-99 19.97603208690288 28.158560901381918 30.04660996342359 21.818797048291607 100 20.331599054938803 27.681492333003575 29.661097677970787 22.32581093408684 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5491.0 1 3698.5 2 6919.0 3 9424.0 4 5037.5 5 3257.0 6 4278.0 7 7164.5 8 10586.0 9 11793.5 10 9955.5 11 7887.5 12 7534.0 13 7955.5 14 8473.0 15 8880.0 16 9188.5 17 9678.5 18 10204.5 19 10746.5 20 11517.0 21 12520.5 22 13728.0 23 15430.5 24 17236.0 25 19714.0 26 25738.5 27 32569.0 28 35969.5 29 44248.5 30 63015.5 31 79369.5 32 101717.0 33 133817.5 34 165653.5 35 193250.0 36 226289.5 37 255421.5 38 255181.5 39 247215.5 40 227421.5 41 202373.0 42 183613.5 43 172485.5 44 174429.0 45 178783.5 46 169567.0 47 149679.5 48 140490.5 49 136426.0 50 132381.5 51 121178.5 52 106061.0 53 97266.0 54 91195.5 55 82875.5 56 73556.0 57 63770.0 58 54855.0 59 47353.0 60 38968.0 61 31475.5 62 25509.5 63 19957.5 64 14784.5 65 10666.0 66 7935.5 67 6188.5 68 4938.5 69 3804.5 70 2964.5 71 2324.0 72 1813.0 73 1460.0 74 1152.0 75 851.5 76 600.0 77 422.0 78 291.0 79 204.5 80 156.0 81 106.0 82 73.5 83 54.5 84 38.5 85 30.0 86 27.0 87 19.5 88 14.5 89 16.0 90 13.5 91 12.0 92 10.0 93 4.0 94 4.5 95 4.0 96 1.5 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025676861505965317 2 0.11042073430095206 3 0.01743162231321311 4 0.017840815325260363 5 0.019948159337303734 6 0.046013754204713944 7 0.046975357783024994 8 0.03893471509629642 9 0.033185553277032466 10-11 0.03670461318063887 12-13 0.03620335174088098 14-15 0.03154878122884344 16-17 0.037615067632444016 18-19 0.03683760090955423 20-21 0.04800857013844432 22-23 0.04756868765049352 24-25 0.06646317498177556 26-27 0.058811265656491876 28-29 0.05041257908422194 30-31 0.04909293162036955 32-33 0.044479280409536734 34-35 0.04608536298182222 36-37 0.04123642578906223 38-39 0.04647409634326711 40-41 0.055885535620354 42-43 0.05606967247577526 44-45 0.06010022364444074 46-47 0.06099021844564352 48-49 0.06036619910227145 50-51 0.05419761444565907 52-53 0.06389548883117903 54-55 0.05106728790349756 56-57 0.055998063698667 58-59 0.05380888108421417 60-61 0.05008522467458414 62-63 0.056939207626375686 64-65 0.05498531099385003 66-67 0.05298026523481848 68-69 0.058852184957696604 70-71 0.0586885077528777 72-73 0.06102090792154706 74-75 0.05160946864446017 76-77 0.052693830126385406 78-79 0.0628725063010609 80-81 0.0630361835058798 82-83 0.05295980558421611 84-85 0.05874988670468479 86-87 0.05666300234324378 88-89 0.06431491166852747 90-91 0.0701254524395985 92-93 0.0745345071444077 94-95 0.07742954770464203 96-97 0.07460611592151596 98-99 0.0829945726684847 100 0.12873212159006675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4887669.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.32952167619588 #Duplication Level Percentage of deduplicated Percentage of total 1 78.3663741377857 36.306766293014185 2 13.39448167653294 12.411198583486827 3 3.830563401606061 5.324045104402519 4 1.539202724857357 2.8524210402135477 5 0.7759026825858033 1.7973600075738752 6 0.4805353328771787 1.335778327242675 7 0.30583677844086987 0.9918490159307939 8 0.21626196168915549 0.8015450593451499 9 0.15416042766287377 0.6427960987516863 >10 0.7433553607208763 6.158535643518865 >50 0.06967090970833079 2.296742684432258 >100 0.09085269167835658 9.49602766129518 >500 0.020222831294313848 6.503773267336503 >1k 0.011977212581361236 9.654788270837532 >5k 3.6112198737772575E-4 1.0780367871756618 >10k+ 2.4074799158515047E-4 2.348336155442764 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30108 0.6159991603359393 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22591 0.4622039667579781 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16238 0.3322238064811672 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15280 0.3126234612041036 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 7310 0.14956004590327207 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7255 0.14843476512014214 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7116 0.1455908736864137 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 6613 0.13529966943342522 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 5257 0.10755638321662125 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 5110 0.10454881457807393 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4967 0.10162308454193605 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 2.0459650602362805E-5 0.0 7 0.0 0.0 0.0 2.0459650602362805E-5 0.0 8 0.0 0.0 0.0 2.0459650602362805E-5 0.0 9 0.0 0.0 0.0 4.091930120472561E-5 0.0 10-11 0.0 0.0 0.0 4.091930120472561E-5 0.0 12-13 0.0 0.0 0.0 4.091930120472561E-5 0.0 14-15 0.0 0.0 0.0 4.091930120472561E-5 3.068947590354421E-5 16-17 0.0 0.0 0.0 4.091930120472561E-5 4.091930120472561E-5 18-19 0.0 0.0 0.0 8.183860240945122E-5 5.114912650590701E-5 20-21 0.0 0.0 0.0 2.4551580722835363E-4 7.160877710826982E-5 22-23 0.0 0.0 0.0 9.104544518051447E-4 1.0229825301181402E-4 24-25 0.0 0.0 0.0 0.002833661608427248 1.2275790361417681E-4 26-27 0.0 0.0 0.0 0.005452496885529687 1.2275790361417681E-4 28-29 0.0 0.0 0.0 0.010945913072264099 1.2275790361417681E-4 30-31 0.0 0.0 0.0 0.029287989837282355 1.2275790361417681E-4 32-33 0.0 0.0 0.0 0.06835569266249412 1.432175542165396E-4 34-35 0.0 0.0 0.0 0.12297272994550162 1.432175542165396E-4 36-37 0.0 1.0229825301181402E-5 0.0 0.2115527872284314 1.432175542165396E-4 38-39 0.0 2.0459650602362805E-5 0.0 0.34270937741487817 1.432175542165396E-4 40-41 0.0 2.0459650602362805E-5 0.0 0.49542020951091414 1.432175542165396E-4 42-43 0.0 2.0459650602362805E-5 0.0 0.6429854394804558 1.432175542165396E-4 44-45 0.0 2.0459650602362805E-5 0.0 0.8132915710945238 1.432175542165396E-4 46-47 0.0 2.0459650602362805E-5 0.0 1.0183177297808013 1.432175542165396E-4 48-49 0.0 2.0459650602362805E-5 0.0 1.2337271611477783 1.432175542165396E-4 50-51 0.0 2.0459650602362805E-5 0.0 1.4572488439785918 1.432175542165396E-4 52-53 0.0 2.0459650602362805E-5 0.0 1.6776708897431476 1.432175542165396E-4 54-55 0.0 2.0459650602362805E-5 0.0 1.9242301391522216 1.432175542165396E-4 56-57 0.0 2.0459650602362805E-5 0.0 2.200752546868456 1.432175542165396E-4 58-59 0.0 2.0459650602362805E-5 0.0 2.510501427162928 1.432175542165396E-4 60-61 0.0 2.0459650602362805E-5 0.0 2.815380910614037 1.432175542165396E-4 62-63 0.0 2.0459650602362805E-5 0.0 3.127267824396456 1.432175542165396E-4 64-65 0.0 2.0459650602362805E-5 0.0 3.4492924950523447 1.432175542165396E-4 66-67 0.0 2.0459650602362805E-5 0.0 3.7991934396539535 1.432175542165396E-4 68-69 0.0 2.0459650602362805E-5 0.0 4.170413340183224 1.432175542165396E-4 70-71 0.0 2.0459650602362805E-5 0.0 4.577877511754581 1.432175542165396E-4 72-73 0.0 2.0459650602362805E-5 0.0 4.967572476777785 1.8413685542126524E-4 74-75 0.0 3.068947590354421E-5 0.0 5.3630370632708555 1.8413685542126524E-4 76-77 0.0 4.091930120472561E-5 0.0 5.785283332402419 1.8413685542126524E-4 78-79 0.0 4.091930120472561E-5 0.0 6.239497805600175 1.8413685542126524E-4 80-81 0.0 4.091930120472561E-5 0.0 6.712463548574995 2.0459650602362803E-4 82-83 0.0 4.091930120472561E-5 0.0 7.184171023037771 2.2505615662599083E-4 84-85 0.0 4.091930120472561E-5 0.0 7.650917032229474 2.2505615662599083E-4 86-87 0.0 4.091930120472561E-5 0.0 8.136690516481373 2.2505615662599083E-4 88 0.0 4.091930120472561E-5 0.0 8.507593292426307 2.2505615662599083E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 6370 0.0 70.15082 2 ACCTGGG 6835 0.0 64.01498 3 GTACCTG 8330 0.0 53.75933 1 GGTATCA 12135 0.0 47.83407 1 CCTGGGG 4320 0.0 45.691097 4 TATCACG 400 0.0 41.158222 2 GTATCAA 22490 0.0 39.551594 1 TAACGCA 650 0.0 36.874218 4 ATCAACG 27015 0.0 32.149 3 CAACGCA 27280 0.0 31.835073 5 GTACACG 645 0.0 31.35961 1 TCAACGC 27790 0.0 31.319761 4 AACGCAG 28895 0.0 30.462967 6 TATCAAC 29220 0.0 29.781055 2 GTACATG 26540 0.0 28.766003 1 CTGGGGG 3575 0.0 28.262505 5 TATAACG 850 0.0 28.222782 2 ACGCAGA 31465 0.0 27.691315 7 CGCAGAG 31885 0.0 27.324598 8 CATGGGT 2840 0.0 27.138857 4 >>END_MODULE