FastQCFastQC Report
Mon 6 Jun 2016
ERR1211350_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211350_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4887669
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260310.5325851648301062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211550.4328239084929851No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147080.3009205410595521No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130090.2661595946861377No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG72800.1489462563852012No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72490.14831200721652796No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT68510.14016906627678757No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62020.1268907530358541No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG64200.070.0113452
ACCTGGG68950.063.8992733
GTACCTG80600.056.1900141
TAACGCA5500.047.773824
CCTGGGG42400.046.920724
TATCACG3350.044.8170362
GGTATCA127950.043.68561
GTATCAA223750.037.251761
GTACACG5550.035.515931
ATCAACG277750.029.57993
CAACGCA279950.029.341145
CTGGGGG36300.029.206075
TATAACG8600.028.9144782
TCAACGC284150.028.8641244
GTACATG271150.028.4377251
AACGCAG294500.028.2100966
TATCAAC297850.027.5662982
GGATGAC52500.026.7167248
ACATGGG272300.026.449983
ATCACGC6150.025.9399073
Produced by FastQC (version 0.11.5)