##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211350_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4887669 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08654125310041 33.0 31.0 34.0 30.0 34.0 2 32.19932241729135 33.0 31.0 34.0 30.0 34.0 3 32.26257813284819 34.0 31.0 34.0 30.0 34.0 4 35.92385020344054 37.0 35.0 37.0 35.0 37.0 5 35.84497640899987 37.0 35.0 37.0 35.0 37.0 6 35.86963172015126 37.0 35.0 37.0 35.0 37.0 7 35.799115692981665 37.0 35.0 37.0 33.0 37.0 8 35.78009476500966 37.0 35.0 37.0 33.0 37.0 9 37.38748470896863 39.0 37.0 39.0 34.0 39.0 10-11 37.32493710191913 39.0 37.0 39.0 34.0 39.0 12-13 37.27213616552184 39.0 37.0 39.0 34.0 39.0 14-15 38.47434533721494 40.0 38.0 41.0 34.0 41.0 16-17 38.47002906702561 40.0 38.0 41.0 34.0 41.0 18-19 38.526005852687646 40.0 38.0 41.0 34.0 41.0 20-21 38.50773344103294 40.0 38.0 41.0 34.0 41.0 22-23 38.36915255513415 40.0 38.0 41.0 34.0 41.0 24-25 38.283020392747545 40.0 38.0 41.0 34.0 41.0 26-27 38.177820654385556 40.0 38.0 41.0 33.5 41.0 28-29 38.07641219157844 40.0 37.0 41.0 33.0 41.0 30-31 38.034973522143176 40.0 37.0 41.0 33.0 41.0 32-33 37.91090036989002 40.0 37.0 41.0 33.0 41.0 34-35 37.75169421251725 40.0 37.0 41.0 33.0 41.0 36-37 37.577296150782715 40.0 36.5 41.0 33.0 41.0 38-39 37.460431138033286 40.0 36.0 41.0 32.0 41.0 40-41 37.358245924591046 40.0 36.0 41.0 32.0 41.0 42-43 37.24897573874172 40.0 36.0 41.0 31.5 41.0 44-45 37.0871541219342 40.0 36.0 41.0 31.0 41.0 46-47 36.82481812905088 39.5 36.0 41.0 30.5 41.0 48-49 35.27403390041347 39.0 35.0 41.0 23.0 41.0 50-51 36.02280811159675 39.0 35.0 40.0 28.0 41.0 52-53 36.151232417743515 39.0 35.0 40.0 29.0 41.0 54-55 36.01189339949166 39.0 35.0 40.0 28.0 41.0 56-57 35.8320225858175 38.5 35.0 40.0 28.0 41.0 58-59 35.64174435707491 38.0 35.0 40.0 28.0 41.0 60-61 35.424016642698184 38.0 34.0 40.0 27.0 41.0 62-63 35.12821786827217 38.0 34.0 40.0 26.5 41.0 64-65 34.83076482879672 37.0 34.0 40.0 26.0 41.0 66-67 34.513382759757256 37.0 34.0 39.5 26.0 41.0 68-69 34.153456177167484 36.0 33.0 39.0 26.0 40.0 70-71 33.80535885306472 36.0 33.0 39.0 25.5 40.0 72-73 33.41468816730429 35.0 32.5 38.0 25.0 40.0 74-75 33.02266274577923 35.0 32.0 37.5 24.5 39.5 76-77 32.05314394243964 34.0 30.5 36.0 23.5 39.0 78-79 32.467790474354956 35.0 32.0 36.0 24.0 39.0 80-81 32.31110862867351 35.0 32.0 36.0 24.0 38.0 82-83 32.0078478104798 35.0 31.5 36.0 24.0 37.0 84-85 31.670405156322985 34.0 31.0 35.0 23.0 37.0 86-87 31.404444020247688 34.0 31.0 35.0 22.0 36.0 88-89 31.15153031025628 34.0 31.0 35.0 20.5 36.0 90-91 30.908948314626052 34.0 31.0 35.0 20.0 36.0 92-93 30.705525067266215 34.0 30.0 35.0 20.0 35.0 94-95 30.49620330263772 34.0 30.0 35.0 19.5 35.0 96-97 30.304656780154303 34.0 29.5 35.0 19.0 35.0 98-99 30.09098416852696 34.0 29.0 35.0 18.0 35.0 100 27.739748129425294 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.26791354550700675 1101 2 -0.19302777753035016 1101 3 -0.20557222666955255 1101 4 -0.16348210024556664 1101 5 -0.153848278498252 1101 6 -0.20911821268936848 1101 7 -0.18145299761215483 1101 8 -0.1884826295842501 1101 9 -0.3238681785344397 1101 10-11 -0.2720088582301301 1101 12-13 -0.3371109345787815 1101 14-15 -0.4128234822275516 1101 16-17 -0.4365192490362517 1101 18-19 -0.4299566925806104 1101 20-21 -0.47128183676167623 1101 22-23 -0.5003341542539985 1101 24-25 -0.42072201025828093 1101 26-27 -0.46284290632583236 1101 28-29 -0.46809676309164416 1101 30-31 -0.4061811772119981 1101 32-33 -0.3403183852295655 1101 34-35 -0.33469178018337686 1101 36-37 -0.48643379431840117 1101 38-39 -0.34780557705690995 1101 40-41 -0.35035939517949544 1101 42-43 -0.3534449984049175 1101 44-45 -0.252126105220178 1101 46-47 -0.3162145436728423 1101 48-49 0.9240127081445522 1101 50-51 0.08815884004357599 1101 52-53 -0.204222042622483 1101 54-55 -0.276253022447591 1101 56-57 -0.29437543585797243 1101 58-59 -0.3600909551778386 1101 60-61 -0.34186727113550575 1101 62-63 -0.3751782516486841 1101 64-65 -0.5036022041367261 1101 66-67 -0.3990626483884867 1101 68-69 -0.5259703277392234 1101 70-71 -0.6044414406325771 1101 72-73 -0.6418479976650886 1101 74-75 -0.5017811648698043 1101 76-77 -0.4543530461351537 1101 78-79 -0.501753150748705 1101 80-81 -0.30212422927633753 1101 82-83 -0.2601018963371011 1101 84-85 -0.30181451469923815 1101 86-87 -0.22304248591519027 1101 88-89 -0.4426147582969584 1101 90-91 -0.4435428801901189 1101 92-93 -0.4047706522157135 1101 94-95 -0.3706631406432521 1101 96-97 -0.4301431480397788 1101 98-99 -0.6691096676046655 1101 100 -0.5812577247979789 1102 1 -0.2352723537749064 1102 2 -0.216972163525476 1102 3 -0.2723972292767982 1102 4 -0.23018278428240535 1102 5 -0.17724809021730437 1102 6 -0.31440480370534374 1102 7 -0.17408054177246868 1102 8 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1105 68-69 -0.6793066817175486 1105 70-71 -0.7226704841739959 1105 72-73 -0.7111378901693044 1105 74-75 -0.7420429061137455 1105 76-77 -0.5926968536196888 1105 78-79 -0.4625381893806022 1105 80-81 -0.5734640238243784 1105 82-83 -0.5620403254348467 1105 84-85 -0.5600693866461661 1105 86-87 -0.5260246148658503 1105 88-89 -0.5964195842599942 1105 90-91 -0.7124173378087804 1105 92-93 -0.6000204955649515 1105 94-95 -0.5704705471579636 1105 96-97 -0.5725664682816038 1105 98-99 -0.5463243546069165 1105 100 -0.6913229660525921 1106 1 -0.2529660202320976 1106 2 -0.30059574852392146 1106 3 -0.3788222418473488 1106 4 -0.13889195369392127 1106 5 -0.27879922500322607 1106 6 -0.3755670967158835 1106 7 -0.3070621992365332 1106 8 -0.3235274623949991 1106 9 -0.4891412089191718 1106 10-11 -0.5221113792333085 1106 12-13 -0.4868744791013526 1106 14-15 -0.6043230250689504 1106 16-17 -0.5772532762393041 1106 18-19 -0.5659093325377356 1106 20-21 -0.5823068184484441 1106 22-23 -0.5513680616178149 1106 24-25 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0.7537544386226109 2310 94-95 0.8946772017447948 2310 96-97 0.9497297778277485 2310 98-99 1.0803437543960115 2310 100 1.3164420697514991 2311 1 0.3531241872300086 2311 2 0.3687531726014157 2311 3 0.37273949540343665 2311 4 0.2251306427857216 2311 5 0.3147302444019573 2311 6 0.3671441336618173 2311 7 0.39802440973245723 2311 8 0.20042593516426876 2311 9 0.44922236818512573 2311 10-11 0.37314478607186885 2311 12-13 0.44927569053772487 2311 14-15 0.3908312740522817 2311 16-17 0.42001043890917344 2311 18-19 0.5529128297735824 2311 20-21 0.5810546050110119 2311 22-23 0.6268146766470934 2311 24-25 0.49143855175866946 2311 26-27 0.5428986813825603 2311 28-29 0.5888425989542512 2311 30-31 0.4828023481852526 2311 32-33 0.5380741072501607 2311 34-35 0.6073456674816171 2311 36-37 0.5728721619800723 2311 38-39 0.6687341392857604 2311 40-41 0.5912010040657592 2311 42-43 0.6011588204113778 2311 44-45 0.5849946008722 2311 46-47 0.6325714138466694 2311 48-49 -6.235774941097709 2311 50-51 -1.2556664744834265 2311 52-53 0.14687584983036572 2311 54-55 0.5066290384803551 2311 56-57 0.6345568840719409 2311 58-59 0.6729338469088191 2311 60-61 0.5463158329065934 2311 62-63 0.44526674627277174 2311 64-65 0.5819269318816964 2311 66-67 0.7712521715656351 2311 68-69 0.7645084020638677 2311 70-71 0.7146394950404371 2311 72-73 0.2779041661005053 2311 74-75 0.5757307307738202 2311 76-77 0.6010594081150522 2311 78-79 0.4816195967597068 2311 80-81 0.5030224982954152 2311 82-83 0.6153518884330289 2311 84-85 0.6612255568756673 2311 86-87 0.6368275051584575 2311 88-89 0.6614420283127629 2311 90-91 0.6807042309733653 2311 92-93 0.6837010482725567 2311 94-95 0.5794872073596089 2311 96-97 0.6812084749259633 2311 98-99 0.7493590155841439 2311 100 0.9293482428081887 2312 1 0.08458692649652022 2312 2 0.2313632329383566 2312 3 0.20757633807632914 2312 4 -0.00798104245698994 2312 5 0.20458902077911745 2312 6 0.14655514051899132 2312 7 0.2730679079046041 2312 8 0.3325035194338426 2312 9 0.292207381437791 2312 10-11 0.24154999830841462 2312 12-13 0.2993540270932371 2312 14-15 0.32700663849391276 2312 16-17 0.4420941620658141 2312 18-19 0.3442881583010049 2312 20-21 0.34190504142718225 2312 22-23 0.5823756822026382 2312 24-25 0.6663221227580394 2312 26-27 0.6312365624331875 2312 28-29 0.6954586775787632 2312 30-31 0.7038635787359269 2312 32-33 0.7105608293501007 2312 34-35 0.29542804491772046 2312 36-37 0.5899201249905559 2312 38-39 0.6880673169554044 2312 40-41 0.45280721956274306 2312 42-43 0.5033141648026813 2312 44-45 0.7177210052057106 2312 46-47 0.7388506638770806 2312 48-49 -4.394676221099495 2312 50-51 -0.6567799214353158 2312 52-53 0.5550565211818181 2312 54-55 0.7472783924760478 2312 56-57 0.8654846290257581 2312 58-59 0.9025581741149509 2312 60-61 0.925037682616221 2312 62-63 0.7625546996565404 2312 64-65 0.9921362782907721 2312 66-67 0.8598896919334109 2312 68-69 0.7963005187871914 2312 70-71 0.8843309117021931 2312 72-73 0.972122907052217 2312 74-75 0.7047237301580651 2312 76-77 0.8480353886236252 2312 78-79 0.4413195696115295 2312 80-81 0.5718020737320018 2312 82-83 0.5957113015437336 2312 84-85 0.7203074306626966 2312 86-87 0.8699611055108001 2312 88-89 0.886259575063665 2312 90-91 0.6540529341035857 2312 92-93 0.8741179825691958 2312 94-95 0.9867588665273246 2312 96-97 0.9360988648449116 2312 98-99 1.0398540872080346 2312 100 0.5607881637969143 2313 1 0.07899146035605042 2313 2 0.21007640637743918 2313 3 0.30974287761406316 2313 4 0.1322279485363893 2313 5 0.14518247271060858 2313 6 0.26337176808630147 2313 7 0.15039752151592012 2313 8 0.2589457282877845 2313 9 0.4264991452123823 2313 10-11 0.2759017400387904 2313 12-13 0.014398294859425675 2313 14-15 0.14200356114125157 2313 16-17 0.07779910638846843 2313 18-19 0.4073667950690023 2313 20-21 0.39134015027451596 2313 22-23 0.4238300563664481 2313 24-25 0.21450630855969877 2313 26-27 0.4070548468394932 2313 28-29 0.3960529813061058 2313 30-31 0.469833421960665 2313 32-33 0.3787054098470506 2313 34-35 0.4016101294094625 2313 36-37 -0.2708186029644253 2313 38-39 0.11058670171542673 2313 40-41 0.25784389481781744 2313 42-43 0.3793412352590053 2313 44-45 0.3353805744275675 2313 46-47 0.21071639139078968 2313 48-49 -5.583581564165076 2313 50-51 -1.4424968901692878 2313 52-53 0.029999793499669636 2313 54-55 0.1366258528115054 2313 56-57 0.060529082521867394 2313 58-59 0.21115378691801823 2313 60-61 0.5267116856147922 2313 62-63 0.6098072257435874 2313 64-65 0.5882486948484029 2313 66-67 0.6596417264766146 2313 68-69 0.67642395873591 2313 70-71 0.6247164261772795 2313 72-73 0.38145524320800916 2313 74-75 0.5325437668301163 2313 76-77 0.5217082042315973 2313 78-79 0.5660650235514737 2313 80-81 -0.3387316468161963 2313 82-83 0.026300843540152385 2313 84-85 0.4043923297312979 2313 86-87 0.5055764191349859 2313 88-89 0.45440205809339673 2313 90-91 0.544859094829139 2313 92-93 0.537263498120236 2313 94-95 0.5587345109300976 2313 96-97 0.6388394859641942 2313 98-99 0.7329874598648729 2313 100 0.8657929127188808 2314 1 0.03200746785274333 2314 2 0.1594425126869794 2314 3 0.1660917477612216 2314 4 0.01441715571693436 2314 5 0.13474318499439875 2314 6 0.2830873289232869 2314 7 0.011199448992854855 2314 8 0.15498300637150209 2314 9 0.32319965825671915 2314 10-11 0.27541713534211 2314 12-13 0.1911724178152454 2314 14-15 0.20703011193434406 2314 16-17 0.3993355986763021 2314 18-19 0.46044444979682453 2314 20-21 0.49687559483449206 2314 22-23 0.2756507585886183 2314 24-25 0.3633025371061862 2314 26-27 0.371097548167981 2314 28-29 0.40305940154277664 2314 30-31 0.3965606141661553 2314 32-33 0.3894864927966566 2314 34-35 0.3311874286800247 2314 36-37 0.3980511346329223 2314 38-39 0.47011518750417025 2314 40-41 0.22260582403077933 2314 42-43 0.2012094520005192 2314 44-45 0.42194592507714646 2314 46-47 0.49743463756527007 2314 48-49 -6.268585072891966 2314 50-51 -1.56024521053439 2314 52-53 -0.9107118148237205 2314 54-55 -0.5525343664166869 2314 56-57 -0.23354712898223795 2314 58-59 0.33781085043148096 2314 60-61 0.6156493543653028 2314 62-63 0.4864930803783807 2314 64-65 0.6280125801460485 2314 66-67 0.5841181223416925 2314 68-69 0.5649735433429868 2314 70-71 0.40898588356549936 2314 72-73 0.5648559086435583 2314 74-75 0.6290187035782893 2314 76-77 0.5321471404638451 2314 78-79 0.29912149449352654 2314 80-81 0.3713721265322718 2314 82-83 0.37577167678943724 2314 84-85 0.4299218479659821 2314 86-87 0.5308544735153653 2314 88-89 0.4187337250870975 2314 90-91 0.5777470368507842 2314 92-93 0.6375564523643575 2314 94-95 0.6546437418770701 2314 96-97 0.6059349325047059 2314 98-99 0.639296031611309 2314 100 -0.5043124591175356 2315 1 0.11757789101082494 2315 2 0.18464448283985746 2315 3 0.3266159465743357 2315 4 0.1471406526059269 2315 5 0.23963381996918542 2315 6 0.3347972837187996 2315 7 0.3475518803663249 2315 8 0.27907989428325664 2315 9 0.4583794383172872 2315 10-11 0.25165618244371046 2315 12-13 0.4465518004036042 2315 14-15 0.2613023364730509 2315 16-17 0.3420147635153512 2315 18-19 0.4503339686484722 2315 20-21 0.37364528177248246 2315 22-23 0.39419415171125394 2315 24-25 0.48371716632165374 2315 26-27 0.45963974694861065 2315 28-29 0.5847921962489195 2315 30-31 0.5628858103300658 2315 32-33 0.5503248306335919 2315 34-35 0.548839818597493 2315 36-37 0.392829393155921 2315 38-39 0.5832131783020174 2315 40-41 0.6281313856573263 2315 42-43 0.6046382514671862 2315 44-45 0.658739465762693 2315 46-47 0.5301730862924998 2315 48-49 -4.893681312188768 2315 50-51 -0.9462708976119671 2315 52-53 0.16409991836810178 2315 54-55 0.13978810532373132 2315 56-57 0.45169299994653045 2315 58-59 0.5303930238691947 2315 60-61 0.45147648584460853 2315 62-63 0.2621833167979304 2315 64-65 0.3994953482276884 2315 66-67 -0.2767262624157354 2315 68-69 0.3308411891895986 2315 70-71 0.3376893064835045 2315 72-73 0.4693079463354337 2315 74-75 0.6714973122209855 2315 76-77 0.5519984132954825 2315 78-79 0.484925716868581 2315 80-81 0.4674954036864065 2315 82-83 0.3745504846495926 2315 84-85 0.4589512630753383 2315 86-87 0.4512649059875038 2315 88-89 0.3942092958291461 2315 90-91 0.5191769710927829 2315 92-93 0.5225107213999713 2315 94-95 0.21581181738595845 2315 96-97 0.3684828039586101 2315 98-99 0.34160747449077533 2315 100 0.5140307388189314 2316 1 0.22381315299300297 2316 2 0.26866796638154966 2316 3 0.16786031984141658 2316 4 -0.05008128482368335 2316 5 0.09992186379315626 2316 6 0.11354355594976795 2316 7 0.2205023276986111 2316 8 0.28296957102909204 2316 9 0.4523647180730208 2316 10-11 0.40077786954684314 2316 12-13 0.3123898328150787 2316 14-15 0.4151978878461051 2316 16-17 0.43506152479969273 2316 18-19 0.11420516089845734 2316 20-21 0.40018819461471367 2316 22-23 0.5382637342385479 2316 24-25 0.5454674310540568 2316 26-27 0.5610718089427635 2316 28-29 0.568729169356601 2316 30-31 0.46430739528203446 2316 32-33 0.453293350863639 2316 34-35 0.4480006075187717 2316 36-37 0.2883303267352204 2316 38-39 0.4145396092601814 2316 40-41 0.002942181500927177 2316 42-43 -0.0583142538555208 2316 44-45 0.20232074328831118 2316 46-47 0.280444853283079 2316 48-49 -5.243726475724166 2316 50-51 -1.0963660516057772 2316 52-53 0.12796175611613592 2316 54-55 0.4287753869428883 2316 56-57 0.3803831825838273 2316 58-59 0.25779310843388714 2316 60-61 0.42096958409307206 2316 62-63 0.35165336555359517 2316 64-65 0.3567463968673579 2316 66-67 0.3561840728121979 2316 68-69 -0.02689524584712899 2316 70-71 -0.44029663182916323 2316 72-73 -0.34040389758657597 2316 74-75 -0.09006616181652305 2316 76-77 0.014657336929072784 2316 78-79 0.12534951322481191 2316 80-81 -0.03265022719991606 2316 82-83 -0.17628741679886062 2316 84-85 0.027875757277417534 2316 86-87 0.1085887700703374 2316 88-89 -0.016741458330802317 2316 90-91 0.055060990235833884 2316 92-93 0.03928678584874845 2316 94-95 0.3974772929127113 2316 96-97 0.35884100654718765 2316 98-99 0.3107336517548447 2316 100 -0.5568186125399066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 9.0 11 5.0 12 41.0 13 144.0 14 622.0 15 2010.0 16 4928.0 17 10746.0 18 17839.0 19 23726.0 20 29516.0 21 38960.0 22 46132.0 23 44292.0 24 41478.0 25 42833.0 26 49400.0 27 60338.0 28 74077.0 29 90667.0 30 112328.0 31 139256.0 32 174904.0 33 227834.0 34 316706.0 35 444653.0 36 670613.0 37 1073303.0 38 1012662.0 39 137622.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.472454358696865 21.35951180597652 14.072313882986846 22.095719952339767 2 14.977732283677222 22.257343067688662 41.834580749652844 20.930343898981274 3 16.80665168200937 31.111802647725927 30.752510502968082 21.32903516729662 4 11.240489657060788 18.65449495571697 40.88392521306565 29.22109017415659 5 11.020037715597713 39.265652719833916 35.611470828517604 14.102838736050764 6 25.768403254885563 40.07487824459435 20.92521423304529 13.231504267474795 7 25.606306648959514 33.370992745618715 25.14422525102467 15.878475354397098 8 25.36323422295334 35.22283467144622 22.354423956722076 17.059507148878357 9 27.11366390782104 16.62425987617831 22.692451632216393 33.56962458378426 10-11 22.887820635120523 27.828769953332127 30.306231648161937 18.977177763385416 12-13 23.824685895289598 26.28910574105838 30.043600605320155 19.842607758331873 14-15 21.791017109726468 27.297907576807994 28.037239099253764 22.873836214211774 16-17 19.688800984687102 29.884142543933233 29.342132937668946 21.08492353371072 18-19 19.555477263292584 28.843994959560476 31.75763334219236 19.842894434954577 20-21 20.30591502342274 28.503132733435365 31.687117640746415 19.50383460239548 22-23 20.471407730306854 28.08175939460582 31.333807384456176 20.11302549063115 24-25 20.08339356144828 28.510572783439137 31.68898047995286 19.717053175159727 26-27 19.685976970198666 28.577714070946325 31.805828834083826 19.930480124771186 28-29 19.624339835694713 27.761396216432203 32.47895852552853 20.135305422344548 30-31 20.218544128311965 28.303207829810813 31.893603419562723 19.584644622314503 32-33 20.16397204560631 28.63240303778799 31.655274433110588 19.548350483495117 34-35 20.203579565755316 28.50984644562487 31.75995930209477 19.52661468652504 36-37 20.372211392103022 28.2361296596514 31.15508439648359 20.23657455176199 38-39 19.891369811068234 28.98011062544983 31.48010090494802 19.648418658533913 40-41 19.86834167327632 28.13168328875122 32.358675281567066 19.641299756405395 42-43 19.838761126298667 28.240598746942908 31.293659398531776 20.626980728226645 44-45 19.482392376599723 28.788724376897235 31.36782079693437 20.361062449568674 46-47 19.80033643873805 28.363400081562144 31.082308810328314 20.753954669371492 48-49 19.948605757472272 28.83954877772741 30.719506969814763 20.492338494985557 50-51 20.048150414281825 28.4309063041641 30.579841081123117 20.941102200430958 52-53 19.823336501084157 28.898400326761276 30.386383746409678 20.891879425744893 54-55 20.089986620071205 28.92046339282992 30.314664659779655 20.67488532731922 56-57 20.113293928380518 29.022018860155047 29.936779859993912 20.927907351470523 58-59 19.58677370574185 28.70850976607599 30.680222075832226 21.024494452349938 60-61 20.294741489930797 29.685733819904094 29.78777168194592 20.231753008219194 62-63 19.927190763310236 29.14338860866099 30.567960743904806 20.36145988412397 64-65 19.8035894347978 28.536226627260408 30.33565067650425 21.324533261437544 66-67 19.90805183913206 28.80265701696696 29.699002186040506 21.59028895786047 68-69 19.560705795698354 29.070035282007918 30.05331442020965 21.315944502084083 70-71 20.03697633528867 28.30455787360286 30.498743596599397 21.15972219450907 72-73 19.855367196952837 28.91481399055052 29.648466058359617 21.581352754137026 74-75 19.70061543513991 29.095897082015902 29.691934582073447 21.511552900770738 76-77 19.516451086685173 28.65464545325817 30.426478809373165 21.40242465068349 78-79 19.349321392606868 28.658040209307828 29.891842071151853 22.10079632693345 80-81 19.23684160721675 29.34349595679197 29.35233479982425 22.06732763616703 82-83 20.047352575437024 28.714023880300154 29.528866327246178 21.70975721701664 84-85 19.2945673636208 28.947668120372622 29.30634169631226 22.451422819694315 86-87 18.968820663124063 29.168867874622062 30.092838023939933 21.76947343831394 88-89 19.46178963021906 28.58211343976626 29.90645674796289 22.049640182051792 90-91 19.61096748884361 28.593460851898666 29.936218207074315 21.859353452183402 92-93 19.64346187853014 28.766735904455537 29.610327186919317 21.97947503009501 94-95 19.06761090063568 27.979785765204362 29.989300946034536 22.96330238812542 96-97 19.557513161152247 28.175474527825124 29.801933098584342 22.465079212438287 98-99 18.980815394339995 28.26388250843533 29.949925177316356 22.80537691990832 100 19.071284659712827 27.730983566697205 29.900047728867285 23.297684044722686 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5345.0 1 3672.5 2 5971.5 3 8216.5 4 4717.0 5 3035.5 6 3997.0 7 6772.5 8 9735.0 9 10735.0 10 9177.5 11 7269.0 12 6967.5 13 7436.5 14 8032.5 15 8488.5 16 8940.0 17 9472.5 18 10162.0 19 10798.0 20 11578.5 21 12618.0 22 13829.0 23 15433.5 24 16922.0 25 19580.0 26 25987.5 27 32787.0 28 36122.0 29 44402.5 30 62930.5 31 79911.5 32 102971.0 33 135210.0 34 166990.0 35 194428.0 36 227580.0 37 257080.0 38 256201.0 39 246614.0 40 226627.5 41 201696.0 42 183166.5 43 172511.0 44 174943.0 45 180330.5 46 171814.0 47 151920.0 48 141348.5 49 136131.5 50 133085.5 51 123009.0 52 107828.5 53 98120.0 54 91603.5 55 83248.5 56 73815.0 57 63927.0 58 54737.5 59 47393.0 60 38861.0 61 30662.5 62 24381.0 63 18684.5 64 13474.0 65 9529.5 66 6928.5 67 5366.5 68 4201.0 69 3227.5 70 2479.5 71 1852.5 72 1441.0 73 1178.0 74 925.0 75 669.5 76 439.0 77 266.5 78 157.0 79 90.0 80 61.0 81 36.5 82 14.5 83 11.0 84 9.0 85 6.5 86 7.0 87 4.5 88 1.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028950405602343368 2 0.0020459650602362807 3 6.137895180708841E-5 4 0.023467219240910137 5 7.365474216850608E-4 6 2.0459650602362805E-5 7 4.091930120472561E-5 8 0.0038668739638465697 9 0.009820632289134146 10-11 9.309141024075076E-4 12-13 1.5344737951772104E-4 14-15 2.1482633132480943E-4 16-17 5.114912650590702E-4 18-19 0.0 20-21 9.20684277106326E-5 22-23 2.1482633132480943E-4 24-25 3.0689475903544204E-5 26-27 4.091930120472561E-5 28-29 0.0012275790361417683 30-31 0.002935959861439062 32-33 0.010117297222868407 34-35 0.01904793471079977 36-37 0.022321478807177816 38-39 0.022301019156575456 40-41 0.005022844222880068 42-43 0.005360428457819055 44-45 0.00927845154817153 46-47 0.012234871060212956 48-49 1.2054416941900117 50-51 0.01839322589152416 52-53 0.01884333820477614 54-55 0.010188905999976676 56-57 0.00780535670480141 58-59 0.008582823427691197 60-61 0.00547295653613205 62-63 0.007713288277090777 64-65 0.01977425230718365 66-67 0.029410747740896526 68-69 0.030024537258967414 70-71 0.026607775608372828 72-73 0.02366158592163258 74-75 0.009749023512025876 76-77 0.014260376469846873 78-79 0.00854190412648647 80-81 0.003099637066257965 82-83 0.014567271228882316 84-85 0.024398133343317644 86-87 0.023477449066211315 88-89 0.03246946550594977 90-91 0.020623327807181707 92-93 0.013216934289126372 94-95 0.00656754784335846 96-97 0.003662277457822942 98-99 0.015999446771047712 100 0.03553841309630419 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4887669.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.91304282511874 #Duplication Level Percentage of deduplicated Percentage of total 1 78.37286542041846 36.76709591795358 2 13.441787188375784 12.61190276028811 3 3.8060472381490267 5.356597712331306 4 1.5641597795649331 2.9351797889623197 5 0.7697571931087477 1.8055826082626938 6 0.4702949632805311 1.3237780651690318 7 0.2926570770554239 0.9610603792282624 8 0.20482533317143864 0.7687183701392731 9 0.15110551983467024 0.6379937750804142 >10 0.7367085249953422 6.166537493379262 >50 0.06809308465927448 2.272384138866104 >100 0.08933835787365962 9.39751793554978 >500 0.019918643988957128 6.351105735504309 >1k 0.011963149542008718 9.768674037809499 >5k 2.3926299084017438E-4 0.7739891855947167 >10k+ 2.3926299084017438E-4 2.1018820958814106 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26031 0.5325851648301062 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21155 0.4328239084929851 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14708 0.3009205410595521 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13009 0.2661595946861377 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 7280 0.1489462563852012 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7249 0.14831200721652796 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 6851 0.14016906627678757 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6202 0.1268907530358541 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.091930120472561E-5 2 0.0 0.0 0.0 0.0 4.091930120472561E-5 3 0.0 0.0 0.0 0.0 4.091930120472561E-5 4 0.0 0.0 0.0 0.0 4.091930120472561E-5 5 0.0 0.0 0.0 0.0 4.091930120472561E-5 6 0.0 0.0 0.0 0.0 4.091930120472561E-5 7 0.0 0.0 0.0 0.0 4.091930120472561E-5 8 0.0 0.0 0.0 0.0 1.0229825301181402E-4 9 0.0 0.0 0.0 6.137895180708841E-5 1.0229825301181402E-4 10-11 0.0 0.0 0.0 6.137895180708841E-5 1.0229825301181402E-4 12-13 0.0 0.0 0.0 6.137895180708841E-5 1.1252807831299542E-4 14-15 0.0 0.0 0.0 6.137895180708841E-5 1.4321755421653964E-4 16-17 0.0 0.0 0.0 7.160877710826982E-5 1.6367720481890244E-4 18-19 0.0 0.0 0.0 1.2275790361417681E-4 1.6367720481890244E-4 20-21 0.0 0.0 0.0 2.864351084330793E-4 1.6367720481890244E-4 22-23 0.0 0.0 0.0 9.513737530098704E-4 1.7390703012008384E-4 24-25 0.0 0.0 0.0 0.0029155002108366994 2.0459650602362803E-4 26-27 0.0 1.0229825301181402E-5 0.0 0.005554795138541501 2.0459650602362803E-4 28-29 0.0 2.0459650602362805E-5 0.0 0.011109590277083001 2.0459650602362803E-4 30-31 0.0 2.0459650602362805E-5 0.0 0.029533505644510703 2.0459650602362803E-4 32-33 0.0 2.0459650602362805E-5 0.0 0.06862166812032484 2.2505615662599083E-4 34-35 0.0 2.0459650602362805E-5 0.0 0.12323870540333234 2.4551580722835363E-4 36-37 0.0 2.0459650602362805E-5 0.0 0.21086738893325224 2.4551580722835363E-4 38-39 0.0 2.0459650602362805E-5 0.0 0.33671469978838586 2.4551580722835363E-4 40-41 0.0 2.0459650602362805E-5 0.0 0.4827352261374491 2.4551580722835363E-4 42-43 0.0 2.0459650602362805E-5 0.0 0.6237022187877288 2.4551580722835363E-4 44-45 0.0 2.0459650602362805E-5 0.0 0.7869395411186806 2.4551580722835363E-4 46-47 0.0 2.0459650602362805E-5 0.0 0.982758857033895 2.4551580722835363E-4 48-49 0.0 4.091930120472561E-5 0.0 1.190680056280407 2.4551580722835363E-4 50-51 0.0 4.091930120472561E-5 0.0 1.4138743847015829 2.4551580722835363E-4 52-53 0.0 4.091930120472561E-5 0.0 1.6337337900745732 2.659754578307164E-4 54-55 0.0 4.091930120472561E-5 0.0 1.8792086780017223 2.864351084330792E-4 56-57 0.0 4.091930120472561E-5 0.0 2.155250283928801 2.864351084330792E-4 58-59 0.0 4.091930120472561E-5 0.0 2.4645183624341174 2.864351084330792E-4 60-61 0.0 4.091930120472561E-5 0.0 2.769264858156311 2.864351084330792E-4 62-63 0.0 4.091930120472561E-5 0.0 3.080537982420659 2.864351084330792E-4 64-65 0.0 5.114912650590701E-5 0.0 3.4021534600645014 2.864351084330792E-4 66-67 0.0 6.137895180708841E-5 0.0 3.7516554414793637 2.966649337342606E-4 68-69 0.0 6.137895180708841E-5 0.0 4.1224252296953825 3.06894759035442E-4 70-71 0.0 7.160877710826982E-5 0.0 4.529265381923366 3.06894759035442E-4 72-73 0.0 8.183860240945122E-5 0.0 4.918745520615246 3.8873336144489327E-4 74-75 0.0 8.183860240945122E-5 0.0 5.314179417632413 3.8873336144489327E-4 76-77 0.0 8.183860240945122E-5 0.0 5.735627760390485 3.8873336144489327E-4 78-79 0.0 8.183860240945122E-5 0.0 6.189392121274988 3.8873336144489327E-4 80-81 0.0 8.183860240945122E-5 0.0 6.661580397526919 3.9896318674607467E-4 82-83 0.0 8.183860240945122E-5 0.0 7.133165114086081 4.2965266264961886E-4 84-85 0.0 8.183860240945122E-5 0.0 7.600412384717542 4.2965266264961886E-4 86-87 0.0 8.183860240945122E-5 0.0 8.086942876041729 4.2965266264961886E-4 88 0.0 8.183860240945122E-5 0.0 8.458612888884252 4.2965266264961886E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 6420 0.0 70.011345 2 ACCTGGG 6895 0.0 63.899273 3 GTACCTG 8060 0.0 56.190014 1 TAACGCA 550 0.0 47.77382 4 CCTGGGG 4240 0.0 46.92072 4 TATCACG 335 0.0 44.817036 2 GGTATCA 12795 0.0 43.6856 1 GTATCAA 22375 0.0 37.25176 1 GTACACG 555 0.0 35.51593 1 ATCAACG 27775 0.0 29.5799 3 CAACGCA 27995 0.0 29.34114 5 CTGGGGG 3630 0.0 29.20607 5 TATAACG 860 0.0 28.914478 2 TCAACGC 28415 0.0 28.864124 4 GTACATG 27115 0.0 28.437725 1 AACGCAG 29450 0.0 28.210096 6 TATCAAC 29785 0.0 27.566298 2 GGATGAC 5250 0.0 26.716724 8 ACATGGG 27230 0.0 26.44998 3 ATCACGC 615 0.0 25.939907 3 >>END_MODULE