FastQCFastQC Report
Mon 6 Jun 2016
ERR1211349_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211349_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14351100
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT785670.5474632606559776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT585100.4077039390708726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368990.25711617924758384No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC208170.14505508288563246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA327950.054.0132141
GTATCAA654700.044.506791
CAACGCA781350.036.2029085
ATCAACG787050.036.0066383
TATCAAC843550.034.3462262
GTACTAG125400.034.207761
TATTCTC88350.033.9849665
AACGCAG871000.033.1243486
GCATCGT123800.031.7692158
TAGGCAT152900.031.6535425
TCAACGC936400.030.4944254
ACGCAGA932000.030.2962917
CGCAGAG937350.030.0530858
TCTCTGC98550.029.8006028
CTGTGCG147400.029.2669289
TAGTACT150550.029.1513524
TACCTGG35450.028.7907262
CTAGGCA168600.028.594494
ACTGTGC156050.028.184728
GCAGAGT983400.028.1460259
Produced by FastQC (version 0.11.5)