FastQCFastQC Report
Mon 6 Jun 2016
ERR1211349_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211349_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14351100
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT674810.4702148267380201No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT532290.37090536613918096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345890.24101985213677No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC200980.14004501397105448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA329200.055.3537941
GTATCAA644650.043.1834141
CAACGCA785300.034.272225
ATCAACG793600.033.9680523
GTACTAG122000.033.3887141
TATCAAC852850.032.4789122
AACGCAG879550.031.1758656
TATTCTC91200.030.6580455
CTGTGCG154000.029.2438439
TACCCCG7800.028.8695265
TCAACGC943300.028.7913824
GGGCACC144550.028.37393294
ACGCAGA946900.028.3341717
TCTCTGC98300.028.3006048
ACTGTGC163650.028.2654178
TAGTACT158550.028.2631764
GCTATTG210950.028.2212738
CGCAGAG949850.028.2017218
ATTTAGG221250.027.5103741
TCAAGAC27350.027.27863
Produced by FastQC (version 0.11.5)