FastQCFastQC Report
Mon 6 Jun 2016
ERR1211348_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211348_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10495020
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332400.3167216451231155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255370.2433249293474429No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156570.14918504204851443No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157250.050.6968461
GTATCAA293050.042.402651
CAACGCA355900.034.3256265
ATCAACG357900.034.0556953
TCAACGC374850.032.7160074
TATCAAC378050.032.531162
AACGCAG380700.032.4355936
ACGCAGA434000.028.1062287
GTACATG417100.028.1002241
CGCAGAG444850.027.2820978
TACATGG431650.026.7265472
ACATGGG433650.025.809753
GCAGAGT490150.024.1389589
GTACTAG35350.023.682771
CATGGGG293100.023.6137084
TACCTGG69350.022.514832
GAGTACT275000.020.97471612-13
ATGGGGG200000.020.6975565
CAGAGTA480450.020.23663110-11
GTGGTAT83700.020.1168271
Produced by FastQC (version 0.11.5)