##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211348_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10495020 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.4037415841037 31.0 31.0 34.0 28.0 34.0 2 31.421448172561842 31.0 31.0 34.0 28.0 34.0 3 31.475335825944114 31.0 31.0 34.0 28.0 34.0 4 35.07917250276798 37.0 35.0 37.0 32.0 37.0 5 35.118796438691874 37.0 35.0 37.0 32.0 37.0 6 35.18338021271041 37.0 35.0 37.0 32.0 37.0 7 35.11568820259514 37.0 35.0 37.0 32.0 37.0 8 35.113647711009605 37.0 35.0 37.0 32.0 37.0 9 36.676422722395955 39.0 37.0 39.0 32.0 39.0 10-11 36.607038909882974 39.0 37.0 39.0 32.0 39.0 12-13 36.52741495490242 39.0 37.0 39.0 32.0 39.0 14-15 37.66667781481121 40.0 37.0 41.0 32.0 41.0 16-17 37.6638798210961 40.0 37.0 41.0 32.0 41.0 18-19 37.66256243437364 40.0 37.0 41.0 32.0 41.0 20-21 37.62730633195554 40.0 37.0 41.0 32.0 41.0 22-23 37.50821999386376 40.0 37.0 41.0 31.5 41.0 24-25 37.39845450508908 40.0 37.0 41.0 31.0 41.0 26-27 37.25564910786259 40.0 36.5 41.0 31.0 41.0 28-29 37.119494388767244 40.0 36.0 41.0 30.5 41.0 30-31 36.93954456494604 39.0 36.0 41.0 30.0 41.0 32-33 36.822865702018674 39.0 36.0 41.0 30.0 41.0 34-35 36.682634763916596 39.0 35.0 41.0 30.0 41.0 36-37 36.58760397788666 39.0 35.0 41.0 30.0 41.0 38-39 36.43948501289183 39.0 35.0 41.0 29.5 41.0 40-41 36.275205811899355 39.0 35.0 40.0 29.0 41.0 42-43 36.12668694295009 39.0 35.0 40.0 28.0 41.0 44-45 35.991442941509405 39.0 35.0 40.0 27.5 41.0 46-47 35.824867175098284 39.0 35.0 40.0 27.0 41.0 48-49 35.592960137284166 38.0 34.0 40.0 26.0 41.0 50-51 34.92366470001963 37.5 33.5 39.5 25.0 40.5 52-53 35.164711453622765 38.0 34.0 40.0 26.0 40.5 54-55 35.5265186726657 38.0 34.0 40.0 26.0 41.0 56-57 35.42687827178986 38.0 34.0 40.0 26.0 41.0 58-59 35.203447730447394 38.0 34.0 40.0 26.0 41.0 60-61 34.936636661959675 37.5 34.0 40.0 26.0 41.0 62-63 34.66181803369598 37.0 33.5 40.0 26.0 41.0 64-65 34.38388483299698 36.5 33.0 40.0 25.0 41.0 66-67 34.1579250920913 36.0 33.0 39.0 25.0 41.0 68-69 33.815245897578095 36.0 33.0 39.0 24.5 41.0 70-71 33.4723391189345 35.0 32.0 39.0 24.0 40.5 72-73 33.10636678157831 35.0 32.0 38.0 23.5 40.0 74-75 32.732885501885654 35.0 32.0 37.0 23.0 39.5 76-77 32.38156182646627 35.0 31.0 37.0 22.0 39.0 78-79 32.017018643127884 35.0 31.0 36.0 21.5 39.0 80-81 31.683741765141942 35.0 31.0 36.0 20.0 37.5 82-83 31.37057061349097 34.0 30.5 35.0 20.0 37.0 84-85 31.06851816385295 34.0 30.0 35.0 20.0 37.0 86-87 30.818997629351827 34.0 30.0 35.0 19.0 36.0 88-89 30.56418515638846 34.0 29.5 35.0 18.5 36.0 90-91 30.308752103378552 34.0 29.0 35.0 18.0 36.0 92-93 30.074033065206166 34.0 29.0 35.0 17.5 35.0 94-95 29.852329628719147 34.0 29.0 35.0 17.0 35.0 96-97 29.652821862178442 34.0 29.0 35.0 15.5 35.0 98-99 29.4179203088703 34.0 27.5 35.0 15.0 35.0 100 27.720300390089776 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.41108665163861957 1101 2 -0.40946286841784385 1101 3 -0.39081396533338975 1101 4 -0.2719713329079596 1101 5 -0.33206417637050123 1101 6 -0.30773176041834205 1101 7 -0.35394030749166205 1101 8 -0.45652247442588134 1101 9 -0.5157310419933552 1101 10-11 -0.4917396891071846 1101 12-13 -0.48676189950267457 1101 14-15 -0.537255050835661 1101 16-17 -0.5734223744326954 1101 18-19 -0.5991403629072138 1101 20-21 -0.6900151044814109 1101 22-23 -0.6483725290374736 1101 24-25 -0.6880638635413376 1101 26-27 -0.7206397433329812 1101 28-29 -0.7612419556504193 1101 30-31 -0.7363032557306397 1101 32-33 -0.7896474212050677 1101 34-35 -0.8566500214405579 1101 36-37 -0.8852032457476184 1101 38-39 -0.8482993235146594 1101 40-41 -0.9172053692668101 1101 42-43 -0.9709704033437703 1101 44-45 -1.067017516414424 1101 46-47 -0.9877319469758703 1101 48-49 -0.9222172370887449 1101 50-51 -0.9455015986725357 1101 52-53 -0.7457147607384229 1101 54-55 -0.6609140886375897 1101 56-57 -0.6360360145378863 1101 58-59 -0.7181661890916757 1101 60-61 -0.7447839676709052 1101 62-63 -0.7534979221325386 1101 64-65 -0.765068176019227 1101 66-67 -0.7856402716108235 1101 68-69 -0.7656964076425723 1101 70-71 -0.8233378483349654 1101 72-73 -0.8443715812703658 1101 74-75 -0.871895282736169 1101 76-77 -0.8863339726162138 1101 78-79 -0.9642013735778967 1101 80-81 -1.0348885985636436 1101 82-83 -1.1552458936334062 1101 84-85 -1.0628765103478948 1101 86-87 -1.1221163269083299 1101 88-89 -1.116007652732236 1101 90-91 -1.1116944917019147 1101 92-93 -1.086209537221201 1101 94-95 -1.2341122144458332 1101 96-97 -1.2586477115884058 1101 98-99 -1.3103700955676238 1101 100 -1.3472266985118502 1102 1 -0.5115353245456902 1102 2 -0.4762415000981939 1102 3 -0.4566916688946314 1102 4 -0.3393979086042265 1102 5 -0.3092282985935668 1102 6 -0.35169387171667665 1102 7 -0.44180082548432864 1102 8 -0.4394844493376624 1102 9 -0.43171556729910066 1102 10-11 -0.45053949177829367 1102 12-13 -0.49998473743057303 1102 14-15 -0.675656442928009 1102 16-17 -0.689141409938081 1102 18-19 -0.6795815388700319 1102 20-21 -0.7233694173176346 1102 22-23 -0.7243701254403234 1102 24-25 -0.7492773197146434 1102 26-27 -0.7121669338908916 1102 28-29 -0.7902347258684372 1102 30-31 -0.8118203053442627 1102 32-33 -0.8271616748944197 1102 34-35 -0.8380336996822422 1102 36-37 -0.9736173701287072 1102 38-39 -1.0240145544395318 1102 40-41 -1.0656304283101008 1102 42-43 -0.9704237457637817 1102 44-45 -0.9801299179226177 1102 46-47 -1.0045745638021728 1102 48-49 -1.0019601333348334 1102 50-51 -0.9252288169393594 1102 52-53 -0.8070550758258861 1102 54-55 -0.7668683866266619 1102 56-57 -0.8436866849899971 1102 58-59 -0.8212876067867043 1102 60-61 -0.8015048789229695 1102 62-63 -0.8018817522984847 1102 64-65 -0.9745919927364284 1102 66-67 -0.8804550038615773 1102 68-69 -0.8438927084595065 1102 70-71 -0.8772027561618216 1102 72-73 -0.9379693872596349 1102 74-75 -0.9589792713867347 1102 76-77 -0.9706044059974275 1102 78-79 -0.9514605498894539 1102 80-81 -0.9930650693803749 1102 82-83 -1.0471277539632204 1102 84-85 -1.0348783476818966 1102 86-87 -1.0455388934442489 1102 88-89 -1.142927638713772 1102 90-91 -1.309633317586357 1102 92-93 -1.2736658374166296 1102 94-95 -1.2677556905074034 1102 96-97 -1.1918420291895195 1102 98-99 -1.2910674494959018 1102 100 -1.458798600983382 1103 1 -0.3668729754129991 1103 2 -0.40629980383706155 1103 3 -0.371211528168768 1103 4 -0.31956354817569377 1103 5 -0.27858558111432075 1103 6 -0.3653039973619059 1103 7 -0.39270855991883735 1103 8 -0.34608512502181554 1103 9 -0.5847967461774317 1103 10-11 -0.5066436703646318 1103 12-13 -0.559650917481413 1103 14-15 -0.6815708406905117 1103 16-17 -0.6534179348050984 1103 18-19 -0.6917912375500563 1103 20-21 -0.7970968810830001 1103 22-23 -0.7250882075395211 1103 24-25 -0.7327235654279178 1103 26-27 -0.7407516952471909 1103 28-29 -0.7619605147531558 1103 30-31 -0.7819960543496407 1103 32-33 -0.8292895483770266 1103 34-35 -0.9003695120311477 1103 36-37 -0.9606157520007912 1103 38-39 -0.959993435368041 1103 40-41 -0.9543280132658438 1103 42-43 -0.9687713996155196 1103 44-45 -0.9867765859668012 1103 46-47 -0.9133525429415599 1103 48-49 -0.8585555834558463 1103 50-51 -0.8873666230208102 1103 52-53 -0.8255224544177295 1103 54-55 -0.8597922761634607 1103 56-57 -0.7806901367145471 1103 58-59 -0.7721808699284267 1103 60-61 -0.7733608223319166 1103 62-63 -0.8478193794803417 1103 64-65 -0.7730458554239092 1103 66-67 -0.8458350709700468 1103 68-69 -0.8300737345105347 1103 70-71 -0.7291174018418616 1103 72-73 -0.8845423379503572 1103 74-75 -0.89597881401113 1103 76-77 -0.8508105052999824 1103 78-79 -0.8585662780474514 1103 80-81 -0.8349501509387913 1103 82-83 -1.015436945753823 1103 84-85 -0.9240035217729634 1103 86-87 -1.0309038158929305 1103 88-89 -1.0689500197604005 1103 90-91 -1.0821126089377096 1103 92-93 -1.071420809617809 1103 94-95 -1.287803853670237 1103 96-97 -1.3562393398070078 1103 98-99 -1.3022695912211013 1103 100 -1.4810648245983735 1104 1 -0.39373336452321794 1104 2 -0.37273335578667144 1104 3 -0.4563131409606349 1104 4 -0.29066484347720234 1104 5 -0.2216559066687367 1104 6 -0.28958534096005195 1104 7 -0.2698104589692747 1104 8 -0.26936004579682304 1104 9 -0.39040689702529363 1104 10-11 -0.3940194390584466 1104 12-13 -0.43716161783071783 1104 14-15 -0.5872711468394343 1104 16-17 -0.49880987612636574 1104 18-19 -0.5695471652070907 1104 20-21 -0.5174186995836081 1104 22-23 -0.521485725414017 1104 24-25 -0.6599900647968582 1104 26-27 -0.6380939319002934 1104 28-29 -0.6065959093144713 1104 30-31 -0.7132908040091905 1104 32-33 -0.719073192350713 1104 34-35 -0.5878054722870871 1104 36-37 -0.7274028909110299 1104 38-39 -0.6905030724291095 1104 40-41 -0.7662845610646656 1104 42-43 -0.7883831272644883 1104 44-45 -0.7949764155364392 1104 46-47 -0.8567577262100698 1104 48-49 -0.8324078998494002 1104 50-51 -0.7456576331276068 1104 52-53 -0.6985352055720568 1104 54-55 -0.7117582015715129 1104 56-57 -0.7009227580378763 1104 58-59 -0.751969543397216 1104 60-61 -0.7149976179255546 1104 62-63 -0.7405538046244686 1104 64-65 -0.705280264793906 1104 66-67 -0.7627779339130925 1104 68-69 -0.6754895634509168 1104 70-71 -0.6930783615060605 1104 72-73 -0.6475056954229856 1104 74-75 -0.7699073154316558 1104 76-77 -0.7651785087934009 1104 78-79 -0.6918181744968805 1104 80-81 -0.8138871200237752 1104 82-83 -0.8307600655240215 1104 84-85 -0.7773929430322646 1104 86-87 -0.8714669988691774 1104 88-89 -0.8166218799126526 1104 90-91 -0.8092550847485533 1104 92-93 -0.8894096141705887 1104 94-95 -0.8766093896701612 1104 96-97 -0.9902779431816207 1104 98-99 -1.0560010659441659 1104 100 -1.1424068402064265 1105 1 -0.4070521880777527 1105 2 -0.40924002445833096 1105 3 -0.40396531621908593 1105 4 -0.3087109279872635 1105 5 -0.3089509568956643 1105 6 -0.2635069098131595 1105 7 -0.3302846250885594 1105 8 -0.32201069749013556 1105 9 -0.47446646520401003 1105 10-11 -0.4907336233113213 1105 12-13 -0.48666406373990156 1105 14-15 -0.5538019809292152 1105 16-17 -0.5646769758529473 1105 18-19 -0.5567231220407294 1105 20-21 -0.5885800549560116 1105 22-23 -0.550296232357347 1105 24-25 -0.5511131677941634 1105 26-27 -0.6184356260663648 1105 28-29 -0.6551520303231868 1105 30-31 -0.6358282845852798 1105 32-33 -0.6032205876979404 1105 34-35 -0.7466618590214082 1105 36-37 -0.7778236369878186 1105 38-39 -0.765583766610483 1105 40-41 -0.7662071680476501 1105 42-43 -0.7466475069680314 1105 44-45 -0.7636843930888588 1105 46-47 -0.7216397587077594 1105 48-49 -0.7597790554474528 1105 50-51 -0.8075739274915534 1105 52-53 -0.6388891010461606 1105 54-55 -0.59874638843268 1105 56-57 -0.5705339695336491 1105 58-59 -0.5307464259890153 1105 60-61 -0.5598763153734865 1105 62-63 -0.6591748607506958 1105 64-65 -0.5922654680333252 1105 66-67 -0.5668414903416306 1105 68-69 -0.5402918426736676 1105 70-71 -0.49657960113695765 1105 72-73 -0.5372712185569455 1105 74-75 -0.6138587032398704 1105 76-77 -0.6468819343193672 1105 78-79 -0.5502325781610402 1105 80-81 -0.6318452431023474 1105 82-83 -0.5718497615479485 1105 84-85 -0.6188960187839889 1105 86-87 -0.6703143959511699 1105 88-89 -0.6456506995249676 1105 90-91 -0.7753772372492129 1105 92-93 -0.8350417975862641 1105 94-95 -0.8258679415505945 1105 96-97 -0.8498255530256777 1105 98-99 -0.8893033589555088 1105 100 -0.9515895456514833 1106 1 -0.49179738285640795 1106 2 -0.5353190667852523 1106 3 -0.47795295875256016 1106 4 -0.42205499664031265 1106 5 -0.364608926500928 1106 6 -0.3562686848382626 1106 7 -0.46938938402291086 1106 8 -0.47792280597776937 1106 9 -0.5720227593808218 1106 10-11 -0.5826620286920772 1106 12-13 -0.5779831766605312 1106 14-15 -0.6639494380497339 1106 16-17 -0.6473880849085631 1106 18-19 -0.7243264217819174 1106 20-21 -0.6593054076124147 1106 22-23 -0.6740783481759891 1106 24-25 -0.7064824584603997 1106 26-27 -0.6935533056064926 1106 28-29 -0.7614463472798647 1106 30-31 -0.7230188988122208 1106 32-33 -0.7768618358897257 1106 34-35 -0.7953045405427162 1106 36-37 -0.8262553961048695 1106 38-39 -0.8804966940707502 1106 40-41 -0.8980918320331597 1106 42-43 -0.8648005236234511 1106 44-45 -0.8390150335463673 1106 46-47 -0.9029625065520932 1106 48-49 -0.8968070154385686 1106 50-51 -0.8999208969487711 1106 52-53 -0.7442120027982355 1106 54-55 -0.8268964581625653 1106 56-57 -0.7375127198180849 1106 58-59 -0.7806410603421838 1106 60-61 -0.8017245880776116 1106 62-63 -0.7293209890154699 1106 64-65 -0.7982623786666849 1106 66-67 -0.8640955622078863 1106 68-69 -0.8131467917630744 1106 70-71 -0.843794444366381 1106 72-73 -0.7336014999813258 1106 74-75 -0.6880707414752436 1106 76-77 -0.6766333325482563 1106 78-79 -0.6709433235968731 1106 80-81 -0.8030335866804812 1106 82-83 -0.7593743351865605 1106 84-85 -0.7455232300292458 1106 86-87 -0.7707455669571956 1106 88-89 -0.8011777201437624 1106 90-91 -0.7710507798206301 1106 92-93 -0.7658393416366209 1106 94-95 -0.9096978120871739 1106 96-97 -0.8333438877948893 1106 98-99 -0.8024675415665357 1106 100 -0.8689705200556546 1107 1 -0.5064353655591489 1107 2 -0.48832275956348425 1107 3 -0.4697057917096501 1107 4 -0.3433810431189741 1107 5 -0.2885280508025261 1107 6 -0.3444147164463658 1107 7 -0.40483820071689536 1107 8 -0.36151161496898965 1107 9 -0.5056871162664223 1107 10-11 -0.45856346218287314 1107 12-13 -0.48185970431463687 1107 14-15 -0.6685665200822655 1107 16-17 -0.6475847769082677 1107 18-19 -0.6157385828560464 1107 20-21 -0.5860722440436561 1107 22-23 -0.6097906940585531 1107 24-25 -0.6792925102880503 1107 26-27 -0.7218752164464561 1107 28-29 -0.6462496215842535 1107 30-31 -0.6392154657394187 1107 32-33 -0.7134764785898469 1107 34-35 -0.7482532629509535 1107 36-37 -0.6962183657698091 1107 38-39 -0.7323913227466932 1107 40-41 -0.8174454960108051 1107 42-43 -0.7696111917722348 1107 44-45 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2208 60-61 0.9601715612622073 2208 62-63 0.9887316524698377 2208 64-65 0.9711699697110419 2208 66-67 0.8971366704427624 2208 68-69 0.9372542858219362 2208 70-71 0.9504402665848204 2208 72-73 0.9553435804231398 2208 74-75 1.0183503756324583 2208 76-77 0.9602635725144282 2208 78-79 0.9647597048647079 2208 80-81 0.9676642294276725 2208 82-83 0.9769039373203618 2208 84-85 0.9445035794461312 2208 86-87 0.9759043767604005 2208 88-89 1.0295626300506306 2208 90-91 1.0951401638788596 2208 92-93 1.133776218006254 2208 94-95 1.1485773802414627 2208 96-97 1.1331269103158768 2208 98-99 1.1241365801694663 2208 100 1.2844867121023533 2209 1 0.5187096831192086 2209 2 0.4071574351873686 2209 3 0.45531498324136166 2209 4 0.417424712968959 2209 5 0.4216332522356083 2209 6 0.46641318836099543 2209 7 0.4995214375963357 2209 8 0.45348437975179223 2209 9 0.5885856185630018 2209 10-11 0.6362363999197029 2209 12-13 0.637004641915702 2209 14-15 0.7958434105739869 2209 16-17 0.7730074450412658 2209 18-19 0.8391972879333025 2209 20-21 0.8617242416338868 2209 22-23 0.8331507239310767 2209 24-25 0.911758843948185 2209 26-27 0.9542072900937839 2209 28-29 0.9311388104725253 2209 30-31 0.965354875473345 2209 32-33 0.9417707332046845 2209 34-35 1.0272175687388199 2209 36-37 1.0241947286878528 2209 38-39 1.0147740613788585 2209 40-41 1.0091949595515501 2209 42-43 1.0648747913837155 2209 44-45 1.1533051203963183 2209 46-47 1.1218931279287503 2209 48-49 1.1000433208059377 2209 50-51 1.0155523699628972 2209 52-53 0.9799957186563901 2209 54-55 0.9923966998871236 2209 56-57 1.0046836776214292 2209 58-59 1.0146105831459025 2209 60-61 0.9766006024943792 2209 62-63 0.9393648960467829 2209 64-65 0.9047267609277654 2209 66-67 0.9211420799589192 2209 68-69 1.0049304369466086 2209 70-71 0.9626684274503106 2209 72-73 0.9280942428678642 2209 74-75 0.8969749357829997 2209 76-77 0.9581811613135116 2209 78-79 1.0049305578691126 2209 80-81 1.023664514832273 2209 82-83 1.0686875005052805 2209 84-85 1.0421136480478985 2209 86-87 1.0969551816255851 2209 88-89 1.1336718316962724 2209 90-91 1.1187151544540086 2209 92-93 1.173353074106636 2209 94-95 1.1844056348803171 2209 96-97 1.186028179345218 2209 98-99 1.138553484993139 2209 100 1.2094030674669405 2210 1 0.6785988797480691 2210 2 0.6985302232345987 2210 3 0.6736159510443329 2210 4 0.571918811715797 2210 5 0.5683501136483287 2210 6 0.575549230293511 2210 7 0.6205859515204182 2210 8 0.6150940033534624 2210 9 0.7479933690533258 2210 10-11 0.7603264461976877 2210 12-13 0.7714270926463698 2210 14-15 1.0042187973265158 2210 16-17 0.984830351263561 2210 18-19 0.9102343543328217 2210 20-21 0.9757420157988506 2210 22-23 1.0179017949081555 2210 24-25 1.0672113063781907 2210 26-27 1.0804602423478329 2210 28-29 1.0727952724717582 2210 30-31 1.092840588820998 2210 32-33 1.1066923581037926 2210 34-35 1.118359136688042 2210 36-37 1.0727252778039187 2210 38-39 1.155999111126171 2210 40-41 1.194830563979437 2210 42-43 1.162478684049816 2210 44-45 1.181946934234027 2210 46-47 1.159155650941436 2210 48-49 1.1949898982574183 2210 50-51 1.1569573039508612 2210 52-53 1.1080836005495271 2210 54-55 1.0551114065382592 2210 56-57 1.0526360172840796 2210 58-59 1.0931261128972878 2210 60-61 1.0894403761722415 2210 62-63 0.9818780618721448 2210 64-65 1.0025756505236174 2210 66-67 1.0398040403787263 2210 68-69 0.9439223945884123 2210 70-71 0.9900879242640883 2210 72-73 0.9963220031201416 2210 74-75 1.0538752958203688 2210 76-77 0.9897868549447892 2210 78-79 1.0112344171792849 2210 80-81 1.054113081963937 2210 82-83 1.0704553291195147 2210 84-85 1.0522061148593203 2210 86-87 1.1098846780699994 2210 88-89 1.1595293386402332 2210 90-91 1.1889427559224615 2210 92-93 1.215641458928431 2210 94-95 1.1749138789732285 2210 96-97 1.343874046104645 2210 98-99 1.3648888576560836 2210 100 1.4368549366511445 2211 1 0.6338457214949038 2211 2 0.6180427820105336 2211 3 0.6568618951482179 2211 4 0.5298010462636853 2211 5 0.5048584852516811 2211 6 0.521449777812677 2211 7 0.523169819393928 2211 8 0.5959510802290282 2211 9 0.6756397377120678 2211 10-11 0.6690042352056409 2211 12-13 0.6952578089303216 2211 14-15 0.9190443681283824 2211 16-17 0.826002981705166 2211 18-19 0.8195454772270949 2211 20-21 0.8597495265131982 2211 22-23 0.9161329864181127 2211 24-25 0.9212614933096219 2211 26-27 0.9301833973781655 2211 28-29 0.9226311499944373 2211 30-31 0.9396609427152711 2211 32-33 0.9165673884631005 2211 34-35 1.0068392373973012 2211 36-37 1.0154443744913522 2211 38-39 1.0708403379261568 2211 40-41 1.110801712380045 2211 42-43 1.0784491105600154 2211 44-45 1.1024596929874875 2211 46-47 1.089299451509774 2211 48-49 1.122868000676867 2211 50-51 1.0692332932027497 2211 52-53 0.9978059739946801 2211 54-55 0.9866215582261688 2211 56-57 0.9349076557580389 2211 58-59 0.9307869512938609 2211 60-61 0.9285799168236579 2211 62-63 0.983324081233107 2211 64-65 0.927975871075013 2211 66-67 0.9820279028963697 2211 68-69 0.9754252093709752 2211 70-71 0.9121504116527035 2211 72-73 0.9200266905280614 2211 74-75 0.9453678660063431 2211 76-77 0.9209239593549228 2211 78-79 0.9134357872578569 2211 80-81 0.9583018093815205 2211 82-83 1.0087449167042202 2211 84-85 1.0274037132687717 2211 86-87 0.9884238967444183 2211 88-89 0.9982491381990357 2211 90-91 1.0962574804945469 2211 92-93 1.0434221408497066 2211 94-95 1.1150712989757956 2211 96-97 1.1882696464880524 2211 98-99 1.1957390383959208 2211 100 1.4040164129142418 2212 1 0.5343735809486319 2212 2 0.4534539097006558 2212 3 0.5057357208171283 2212 4 0.4616593600712946 2212 5 0.2939640695795589 2212 6 0.39339662437524936 2212 7 0.4719209536855402 2212 8 0.5031351388279148 2212 9 0.6340905287945304 2212 10-11 0.6077918943568434 2212 12-13 0.6958137739153116 2212 14-15 0.7924414399502169 2212 16-17 0.789892744007183 2212 18-19 0.8210089484295224 2212 20-21 0.8556959828309303 2212 22-23 0.8796440791596325 2212 24-25 0.9304906937115689 2212 26-27 0.9130480627680058 2212 28-29 0.9123142242964306 2212 30-31 0.9118906372346558 2212 32-33 0.9241101708043615 2212 34-35 0.8821354181450545 2212 36-37 0.9462790977647728 2212 38-39 0.9728968939135783 2212 40-41 0.9828494448986547 2212 42-43 1.0725514902712732 2212 44-45 1.0717404447704695 2212 46-47 1.0373510335991298 2212 48-49 1.1058785172927799 2212 50-51 1.0732519022015694 2212 52-53 0.9977100900727365 2212 54-55 0.9384546548871597 2212 56-57 0.9267494210203537 2212 58-59 0.9932716385725158 2212 60-61 0.9808516769581175 2212 62-63 0.9143686346988318 2212 64-65 0.9907613897666678 2212 66-67 1.0194882887089207 2212 68-69 0.9781279917005605 2212 70-71 1.0088498971876945 2212 72-73 0.9955410600692929 2212 74-75 1.0143586284261232 2212 76-77 0.9828581842175694 2212 78-79 0.9755220653384988 2212 80-81 1.009329534797974 2212 82-83 1.036983298546918 2212 84-85 1.0480627206864774 2212 86-87 1.0483463724422215 2212 88-89 1.0512800705392067 2212 90-91 1.0499612417775666 2212 92-93 1.0692562481005865 2212 94-95 1.2196827163052397 2212 96-97 1.222748379111966 2212 98-99 1.280755555649499 2212 100 1.4588898340714067 2213 1 0.5050120662445288 2213 2 0.5287012525144377 2213 3 0.5391330770230027 2213 4 0.42733669679633834 2213 5 0.4223786398146103 2213 6 0.4442431509561118 2213 7 0.5107353507170274 2213 8 0.5031750215723889 2213 9 0.5904211153892049 2213 10-11 0.6070296342616004 2213 12-13 0.6097852655156046 2213 14-15 0.7810280800266511 2213 16-17 0.7398982756668673 2213 18-19 0.7167677049228658 2213 20-21 0.7117957857133703 2213 22-23 0.7639923981156542 2213 24-25 0.7762598285899998 2213 26-27 0.8150907944476771 2213 28-29 0.8451599213892749 2213 30-31 0.8540454261189936 2213 32-33 0.8682157046949754 2213 34-35 0.9093206031079717 2213 36-37 0.9268278905909426 2213 38-39 0.9425486074430154 2213 40-41 0.9615849655511184 2213 42-43 0.9619304362265666 2213 44-45 1.0107439717253683 2213 46-47 1.0114331955752576 2213 48-49 1.0688869228062288 2213 50-51 1.0503236914025464 2213 52-53 0.8976240688507318 2213 54-55 0.9153255593878882 2213 56-57 0.9182475144894866 2213 58-59 0.9163735519657763 2213 60-61 0.9512806441202812 2213 62-63 0.9155757269910509 2213 64-65 0.9029521109554892 2213 66-67 0.8833127444895013 2213 68-69 0.9196693991087344 2213 70-71 0.8746693186361085 2213 72-73 0.8921059809450398 2213 74-75 0.9324313597052694 2213 76-77 0.8864285574310884 2213 78-79 0.8758248030273244 2213 80-81 0.8782537437543247 2213 82-83 0.8587008097331967 2213 84-85 0.9257438730825243 2213 86-87 0.9826384909100199 2213 88-89 1.0030895396968802 2213 90-91 1.0010315102939806 2213 92-93 0.9862822236785647 2213 94-95 1.0205149538524516 2213 96-97 1.0628613593471137 2213 98-99 1.0985661527960744 2213 100 1.3018495120557532 2214 1 0.39876526979728766 2214 2 0.3680599605792594 2214 3 0.3593934325442021 2214 4 0.26813017590858124 2214 5 0.2882900366831578 2214 6 0.32606519248616195 2214 7 0.27531871876242064 2214 8 0.3110863373643369 2214 9 0.3503075149778354 2214 10-11 0.39105270802676984 2214 12-13 0.42142397267324583 2214 14-15 0.5257398978593812 2214 16-17 0.5294546880087196 2214 18-19 0.5396504224499026 2214 20-21 0.5677660465849144 2214 22-23 0.601198813726775 2214 24-25 0.6062221020903493 2214 26-27 0.630693742749898 2214 28-29 0.6288125339919972 2214 30-31 0.6995922778930819 2214 32-33 0.6671388450846649 2214 34-35 0.6866004528917315 2214 36-37 0.6742778779619556 2214 38-39 0.7072327679975814 2214 40-41 0.7333268599863487 2214 42-43 0.8427196085086024 2214 44-45 0.8390309038054156 2214 46-47 0.8252674543712928 2214 48-49 0.8380791006423394 2214 50-51 0.8255755586311793 2214 52-53 0.7800680675212845 2214 54-55 0.690906417714686 2214 56-57 0.713320681264662 2214 58-59 0.7030553298519422 2214 60-61 0.6677266240233095 2214 62-63 0.6670981406743266 2214 64-65 0.6616894584689632 2214 66-67 0.6510799930581541 2214 68-69 0.6497790178374672 2214 70-71 0.7010554736522892 2214 72-73 0.7659515930527974 2214 74-75 0.7743544564507729 2214 76-77 0.8201160934702969 2214 78-79 0.7718954157603015 2214 80-81 0.7733353708367368 2214 82-83 0.8309310725724579 2214 84-85 0.847356832759079 2214 86-87 0.8489300321913227 2214 88-89 0.8479114127001921 2214 90-91 0.8090550877305276 2214 92-93 0.8855529290187505 2214 94-95 0.9559514926026154 2214 96-97 1.033369540399498 2214 98-99 1.0469585536866504 2214 100 1.036050030654792 2215 1 0.34256904669737764 2215 2 0.16454278622957474 2215 3 0.2982458815786053 2215 4 0.23721819708239877 2215 5 0.2632417778905918 2215 6 0.27939183638645915 2215 7 0.35285057111788376 2215 8 0.3623619643031901 2215 9 0.36277107309253154 2215 10-11 0.40648476136474443 2215 12-13 0.39546659070040135 2215 14-15 0.5969127737206392 2215 16-17 0.5541851367877726 2215 18-19 0.5478257740910379 2215 20-21 0.5376513818992166 2215 22-23 0.6061396808319657 2215 24-25 0.5903785105825037 2215 26-27 0.674427760419654 2215 28-29 0.668085723316004 2215 30-31 0.6905712568596059 2215 32-33 0.7175633145967311 2215 34-35 0.7326826697412514 2215 36-37 0.8203999835463378 2215 38-39 0.8139662390732738 2215 40-41 0.7685601957195161 2215 42-43 0.7942017897335631 2215 44-45 0.8367749995805269 2215 46-47 0.8679185095724904 2215 48-49 0.8698489165804233 2215 50-51 0.5723630149606365 2215 52-53 0.6115550680392161 2215 54-55 0.7180445032766087 2215 56-57 0.7351338210478175 2215 58-59 0.7070442065594449 2215 60-61 0.7153302470510212 2215 62-63 0.768274835164064 2215 64-65 0.7586077925034544 2215 66-67 0.7292925452634762 2215 68-69 0.7608828967383445 2215 70-71 0.7538314306558362 2215 72-73 0.7435308402540883 2215 74-75 0.7807480423453015 2215 76-77 0.7854451298959617 2215 78-79 0.7563069985301105 2215 80-81 0.8200425343880617 2215 82-83 0.8753083432748987 2215 84-85 0.7173442661264069 2215 86-87 0.8357755517963916 2215 88-89 0.8988724374203159 2215 90-91 1.0204577044369465 2215 92-93 1.0257400731834068 2215 94-95 1.0593732730615386 2215 96-97 1.0740724528755443 2215 98-99 1.110620075323169 2215 100 1.142122966542395 2216 1 0.15794353797769034 2216 2 0.01850257669886446 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2311 10-11 0.555647670708808 2311 12-13 0.5720795282677145 2311 14-15 0.7318675012848885 2311 16-17 0.7164916514785489 2311 18-19 0.5988713690704159 2311 20-21 0.7083397508574407 2311 22-23 0.7814472670008357 2311 24-25 0.7593844155483396 2311 26-27 0.8437054792059726 2311 28-29 0.8155304393911322 2311 30-31 0.8799064418885578 2311 32-33 0.8753052149579688 2311 34-35 0.8734681133972515 2311 36-37 0.8908493974091627 2311 38-39 0.8764471967424257 2311 40-41 0.9785828048394407 2311 42-43 0.9787269459184955 2311 44-45 0.9924093270837844 2311 46-47 0.9806986442276724 2311 48-49 0.9180802621097826 2311 50-51 0.9578111355152288 2311 52-53 0.8222176263172756 2311 54-55 0.8328119623579227 2311 56-57 0.8066708327323866 2311 58-59 0.7741356076719512 2311 60-61 0.7951304789172369 2311 62-63 0.7969764699498967 2311 64-65 0.7982283077704224 2311 66-67 0.8003004346811551 2311 68-69 0.8177296849473663 2311 70-71 0.7670981061484952 2311 72-73 0.7167443150404935 2311 74-75 0.7533496257148968 2311 76-77 0.7985541387215349 2311 78-79 0.8158012328660682 2311 80-81 0.8528547520682785 2311 82-83 0.8191914904503896 2311 84-85 0.8943072488594517 2311 86-87 0.8642171513832508 2311 88-89 0.8925888972614473 2311 90-91 0.9369965020087925 2311 92-93 0.9799118445411565 2311 94-95 1.0351150511665814 2311 96-97 1.0370233127651112 2311 98-99 1.0159203790277864 2311 100 1.1238303976852109 2312 1 0.43737144618366486 2312 2 0.46103409013936414 2312 3 0.46482996849186264 2312 4 0.38447452856973285 2312 5 0.34635034893440064 2312 6 0.3595301250073746 2312 7 0.39592306815390543 2312 8 0.35527219966284207 2312 9 0.4882681415965635 2312 10-11 0.510187131514023 2312 12-13 0.5342770515066775 2312 14-15 0.7192422696948668 2312 16-17 0.6691655474787837 2312 18-19 0.6525442665617689 2312 20-21 0.6706230368074344 2312 22-23 0.7131679713035339 2312 24-25 0.6978224601974858 2312 26-27 0.7431510683059557 2312 28-29 0.701606788444856 2312 30-31 0.7746954623023825 2312 32-33 0.7280134929693389 2312 34-35 0.7806438960853868 2312 36-37 0.8017015329394894 2312 38-39 0.819065344298636 2312 40-41 0.9045962343258154 2312 42-43 0.8926621916276005 2312 44-45 0.8862593403822174 2312 46-47 0.874336615010229 2312 48-49 0.899155225847295 2312 50-51 0.895338830018062 2312 52-53 0.7540923181604597 2312 54-55 0.7553146361261796 2312 56-57 0.7406278216036029 2312 58-59 0.7049289744315743 2312 60-61 0.7652834745970196 2312 62-63 0.7044471078309584 2312 64-65 0.6926849835639857 2312 66-67 0.6901751818833546 2312 68-69 0.7614742655831606 2312 70-71 0.7452560402698936 2312 72-73 0.7349674514062627 2312 74-75 0.7454305346742913 2312 76-77 0.7469672363869577 2312 78-79 0.7269137441847029 2312 80-81 0.7785178156142258 2312 82-83 0.7882169591384489 2312 84-85 0.7390529545874074 2312 86-87 0.8177057825520855 2312 88-89 0.831009079384124 2312 90-91 0.9202860837971478 2312 92-93 0.9952492371777417 2312 94-95 0.953747919025485 2312 96-97 0.9856081158187919 2312 98-99 1.1328463106326438 2312 100 1.293493155585054 2313 1 0.4639107745858695 2313 2 0.4371863321738907 2313 3 0.495404395917042 2313 4 0.40211076903576526 2313 5 0.386492339254346 2313 6 0.4286895766719212 2313 7 0.4654604919379608 2313 8 0.41330830570790056 2313 9 0.5380443342584513 2313 10-11 0.5493976590881218 2313 12-13 0.5925015863334409 2313 14-15 0.7617684950452528 2313 16-17 0.7562166690369807 2313 18-19 0.6772855407656593 2313 20-21 0.696629328751861 2313 22-23 0.6435834506440088 2313 24-25 0.6253120371198975 2313 26-27 0.6850800188629691 2313 28-29 0.7500502334428347 2313 30-31 0.8315211328962206 2313 32-33 0.8451885342466099 2313 34-35 0.8326712237627163 2313 36-37 0.8599514135976989 2313 38-39 0.8716677278869511 2313 40-41 0.9304239805740053 2313 42-43 0.7209289959946972 2313 44-45 0.8498035483041733 2313 46-47 0.93748774238545 2313 48-49 0.9501861438056807 2313 50-51 0.8776637692876577 2313 52-53 0.795623320587751 2313 54-55 0.7833791141601196 2313 56-57 0.8104404547461854 2313 58-59 0.829222486039761 2313 60-61 0.7753544694129957 2313 62-63 0.7854895439011074 2313 64-65 0.7557454018961138 2313 66-67 0.7277417804806845 2313 68-69 0.714296983841713 2313 70-71 0.7316955594847911 2313 72-73 0.7405716183875413 2313 74-75 0.7490317548930747 2313 76-77 0.8044424489515549 2313 78-79 0.8087194963881252 2313 80-81 0.8160403101256399 2313 82-83 0.8254680695579744 2313 84-85 0.8657572441732988 2313 86-87 0.8747717328664848 2313 88-89 0.8478516905427753 2313 90-91 0.9857928839825938 2313 92-93 0.9987377633902241 2313 94-95 1.0401363819387477 2313 96-97 1.078696254606072 2313 98-99 1.0362851496587169 2313 100 1.04305673290974 2314 1 0.32810672122069917 2314 2 0.2123433041281082 2314 3 0.28590557367521185 2314 4 0.23866995711090055 2314 5 0.20298795228879385 2314 6 0.26044007998356733 2314 7 0.3261238867945764 2314 8 0.36218251156193304 2314 9 0.42211549664809667 2314 10-11 0.42046201679098516 2314 12-13 0.4749579898852616 2314 14-15 0.506976058177834 2314 16-17 0.5526896906552068 2314 18-19 0.5425264185561005 2314 20-21 0.5705502101202953 2314 22-23 0.5805519740976735 2314 24-25 0.5803423417066895 2314 26-27 0.5926837753859573 2314 28-29 0.6060214739104524 2314 30-31 0.6603935954671485 2314 32-33 0.6670127210189989 2314 34-35 0.6339490912911501 2314 36-37 0.6500615661645526 2314 38-39 0.7010018344141855 2314 40-41 0.6927592094784032 2314 42-43 0.5947772013492738 2314 44-45 0.6911530302791604 2314 46-47 0.5242674073678515 2314 48-49 0.4428472177359808 2314 50-51 0.6065762406429016 2314 52-53 0.5962209144066222 2314 54-55 0.6547748200296084 2314 56-57 0.5931474997848412 2314 58-59 0.6211444260846051 2314 60-61 0.46890756914078935 2314 62-63 0.59381365014832 2314 64-65 0.5875786254366702 2314 66-67 0.607328842899733 2314 68-69 0.7020040377883916 2314 70-71 0.6602117818501299 2314 72-73 0.6594857358780359 2314 74-75 0.757425178997913 2314 76-77 0.7435014868508816 2314 78-79 0.6918639622861527 2314 80-81 0.7245473266384543 2314 82-83 0.7317915382597384 2314 84-85 0.8146966365796402 2314 86-87 0.7906646728287363 2314 88-89 0.6726091814077648 2314 90-91 0.6803164101864816 2314 92-93 0.9180853048954738 2314 94-95 0.9076033206552871 2314 96-97 0.9333268646472526 2314 98-99 0.8492792915081111 2314 100 0.8916193495985247 2315 1 0.323617277205809 2315 2 0.3532216528044252 2315 3 0.3781200500359745 2315 4 0.24511512565064209 2315 5 0.27917297389511475 2315 6 0.24713494064988595 2315 7 0.21983486471244618 2315 8 0.2119845475618547 2315 9 0.15309912632451983 2315 10-11 0.30341016111753305 2315 12-13 0.3129528101645178 2315 14-15 0.4214734330187966 2315 16-17 0.3047199838160779 2315 18-19 0.44657925433610757 2315 20-21 0.3545997300163606 2315 22-23 0.31348562659606927 2315 24-25 0.4990771736016484 2315 26-27 0.6105512003476505 2315 28-29 0.42188069307032805 2315 30-31 0.44927181069935074 2315 32-33 0.5276486799269691 2315 34-35 0.47099748954902765 2315 36-37 0.5930345661414691 2315 38-39 0.6806745391460112 2315 40-41 0.7486826300822713 2315 42-43 0.7077856802734459 2315 44-45 0.695214381545675 2315 46-47 0.6944465072232973 2315 48-49 0.6585038040368261 2315 50-51 0.5968911872762064 2315 52-53 0.30710626047421385 2315 54-55 0.5172051877304682 2315 56-57 0.4955398055861693 2315 58-59 0.513035310588215 2315 60-61 0.6448180732983602 2315 62-63 0.5949140316266153 2315 64-65 0.5818304799643954 2315 66-67 0.4536593067915575 2315 68-69 0.4746235911583696 2315 70-71 0.4708505494870323 2315 72-73 0.5364109544390985 2315 74-75 0.25999556865976814 2315 76-77 0.5415557268268643 2315 78-79 0.5428990454503015 2315 80-81 0.5733127276809675 2315 82-83 0.5733481671717193 2315 84-85 0.5735771449612592 2315 86-87 0.5309233084065177 2315 88-89 0.48925066948482154 2315 90-91 0.6595448639753059 2315 92-93 0.8365322176440877 2315 94-95 0.7519802672417555 2315 96-97 0.7140806823366539 2315 98-99 0.8530900239528378 2315 100 0.945949629499232 2316 1 0.15681977445758832 2316 2 0.19313487123099193 2316 3 0.19665496800415738 2316 4 0.1112871539417668 2316 5 0.09788427582612513 2316 6 -0.0338216478221014 2316 7 0.003679056113099932 2316 8 0.038654698765888895 2316 9 0.12050990764905833 2316 10-11 0.17477846427228627 2316 12-13 0.14392536943276468 2316 14-15 0.04753470280081018 2316 16-17 0.1876113201776448 2316 18-19 0.22019284403182837 2316 20-21 0.19945966405904159 2316 22-23 0.15236451818132224 2316 24-25 0.15663758261996463 2316 26-27 0.09640937138036776 2316 28-29 0.08835031744549582 2316 30-31 -0.3991844977651695 2316 32-33 -0.23251351699087053 2316 34-35 -0.3592635603752683 2316 36-37 -0.040678498799522345 2316 38-39 0.03143379494839138 2316 40-41 0.294273570285128 2316 42-43 0.2698943231705897 2316 44-45 0.13651311840087743 2316 46-47 -0.017085735117980505 2316 48-49 0.14696600302880825 2316 50-51 0.28952653458917865 2316 52-53 -0.10900109646271972 2316 54-55 -0.0050559451590075355 2316 56-57 -0.0015309429671219732 2316 58-59 -0.36259325432165923 2316 60-61 0.11089037744869046 2316 62-63 0.07805160862866956 2316 64-65 -0.24378211414922646 2316 66-67 -0.54918158674338 2316 68-69 -0.48509963701660297 2316 70-71 -0.29543182248733046 2316 72-73 -0.5087306463739552 2316 74-75 -0.3204695341133359 2316 76-77 0.021829097079695714 2316 78-79 -0.001753558619135731 2316 80-81 -0.07380369320136637 2316 82-83 0.1948557273733229 2316 84-85 0.19068589156487192 2316 86-87 0.31057045751567713 2316 88-89 0.3450488981783266 2316 90-91 -0.274633137808447 2316 92-93 -0.37627926473618345 2316 94-95 0.025189899498617052 2316 96-97 0.30619276828857167 2316 98-99 0.3766302309509513 2316 100 0.29910367508859537 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 319.0 1 803.0 2 474.0 3 458.0 4 394.0 5 374.0 6 432.0 7 1124.0 8 2834.0 9 5043.0 10 7936.0 11 12469.0 12 18463.0 13 23757.0 14 28048.0 15 31927.0 16 37703.0 17 45666.0 18 54958.0 19 65774.0 20 79587.0 21 96249.0 22 108291.0 23 111149.0 24 112718.0 25 117544.0 26 129122.0 27 147114.0 28 172993.0 29 205598.0 30 248642.0 31 306848.0 32 387329.0 33 501846.0 34 687334.0 35 957072.0 36 1420643.0 37 2051643.0 38 1883677.0 39 430288.0 40 377.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.35740625365446 18.863771808909156 13.091059191910585 23.687762745525802 2 15.77727550437857 19.733265162358364 40.670285388057074 23.81917394520599 3 19.251439740556037 25.259315309614504 29.080815117585562 26.408429832243897 4 12.796809646234284 16.061859896726723 37.801310209507534 33.340020247531456 5 13.59346713037034 37.527451875114124 34.4535947518076 14.425486242707933 6 31.59345978321869 36.5103858290918 17.753653893652793 14.142500494036717 7 28.33477612678737 31.94488652932341 21.714989999587228 18.005347344301995 8 26.288829209528508 35.373054571790846 19.854191422300403 18.483924796380247 9 26.778173125405687 15.942046252813 20.351599918791173 36.92818070299014 10-11 25.408838536962524 26.048919695343425 27.73633706273556 20.80590470495849 12-13 25.680476993733897 23.624703048943747 28.085047792880268 22.60977216444209 14-15 23.134660321543997 25.114329253763096 25.659583050409335 26.091427374283573 16-17 22.48499257409203 28.03395833732486 26.53212433203288 22.948924756550227 18-19 22.26582535600921 27.303413915673787 28.56819657745796 21.862564150859047 20-21 23.073304200781525 26.491940629004013 28.483157011629174 21.951598158585288 22-23 23.116106441315317 26.272271298651596 28.202651104313798 22.408971155719286 24-25 22.70108233969127 26.410869554542636 28.512543438444045 22.37550466732205 26-27 22.56024518379608 26.624967840649223 28.284159141605013 22.530627833949683 28-29 22.401242300152404 26.635103263721515 28.51077991771735 22.45287451840873 30-31 22.86338213117757 26.555754516529838 28.345945696539587 22.234917655753005 32-33 22.72382320905876 26.719198540818702 28.140039537605627 22.416938712516917 34-35 22.864597458667237 26.672054252721477 28.288420630180745 22.17492765843054 36-37 22.828398910968588 26.538308342801724 28.114613773760368 22.51867897246932 38-39 22.738800064784996 26.74655672409837 28.24447146891021 22.270171742206422 40-41 22.757115018667033 26.337529125528647 28.517043439462757 22.388312416341567 42-43 22.58544558177874 26.418723118565968 28.205242244005994 22.790589055649296 44-45 22.51825625475868 26.81611050485216 28.126497440015164 22.539135800374 46-47 22.590784814394056 26.636396759143764 27.850676231914225 22.922142194547956 48-49 22.747341116634004 26.700355096189742 27.790513603551066 22.761790183625187 50-51 22.706013788220908 26.766883043802174 27.659813519095717 22.867289648881194 52-53 22.57085924488951 27.01793588359968 27.528291400495476 22.882913471015335 54-55 22.517518150927916 26.897035624510373 27.62230530548352 22.96314091907819 56-57 22.708001289919395 26.883154801529503 27.399432406865177 23.00941150168593 58-59 22.48810077041407 26.81060129345058 27.756209357417532 22.94508857871782 60-61 22.678038453926558 27.038448587959707 27.498807395179476 22.784705562934263 62-63 22.619684515645297 26.9189929429814 27.544743765075136 22.91657877629817 64-65 22.432500533406095 26.69924876973388 27.621941209210167 23.24630948764986 66-67 22.549860371178855 26.68300694485657 27.41100733191333 23.356125352051247 68-69 22.273332399954864 26.787375670103053 27.608243419383864 23.33104851055821 70-71 22.423311095075952 26.480951292939253 27.69396925174114 23.401768360243658 72-73 22.515421140936798 26.709242101484566 27.45219797887055 23.32313877870809 74-75 22.474696967068066 26.85201250703802 27.365270871105395 23.308019654788513 76-77 22.373666701114153 26.686865478241074 27.42966801901654 23.509799801628233 78-79 22.306564239018776 26.597988008368173 27.48308736752161 23.612360385091446 80-81 22.243054463728715 26.91865770593719 27.35395774503796 23.484330085296136 82-83 22.620462099693068 26.560526708420344 27.62775286613638 23.191258325750205 84-85 22.567892446763295 26.637768643310405 27.31608769876826 23.478251211158042 86-87 22.20240670788105 26.813500517757905 27.60703845671105 23.377054317649993 88-89 22.40883715145152 26.420384349460157 27.597520062169593 23.57325843691873 90-91 22.52557582373218 26.38982293370772 27.602490421729936 23.482110820830165 92-93 22.561489365313456 26.4531045671548 27.497225638589434 23.488180428942307 94-95 22.275098062064366 26.223575403800957 27.68679662270188 23.814529911432796 96-97 22.36439544130715 26.16478733121334 27.609195996349428 23.861621231130083 98-99 22.22502273108835 26.244933872308906 27.55463745910752 23.975405937495218 100 22.53751367425161 26.057327576583916 27.270374181715418 24.134784567449053 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1929.0 1 1452.5 2 1667.5 3 2262.5 4 1762.0 5 1552.5 6 2279.5 7 4041.0 8 6566.5 9 8003.0 10 7451.0 11 6630.0 12 6993.5 13 7825.5 14 8571.0 15 9426.5 16 10231.5 17 11043.5 18 11866.0 19 12804.0 20 14029.0 21 15443.5 22 17562.5 23 20641.0 24 24383.5 25 29173.0 26 36339.5 27 45400.0 28 54647.0 29 68754.0 30 89051.5 31 109089.5 32 134721.5 33 165035.5 34 195458.5 35 226174.0 36 261898.0 37 298306.5 38 315839.0 39 322604.5 40 323147.5 41 322011.0 42 327405.5 43 340598.0 44 358980.0 45 382763.0 46 402361.0 47 412390.5 48 423495.0 49 429618.5 50 424356.0 51 416808.5 52 406540.5 53 391510.0 54 374871.0 55 347026.5 56 314229.0 57 277089.5 58 240385.5 59 210160.0 60 176930.0 61 144489.5 62 117186.0 63 92740.0 64 69502.0 65 49856.0 66 36954.0 67 28338.5 68 21686.0 69 16258.5 70 12292.0 71 9350.5 72 7102.0 73 5377.0 74 4010.5 75 2880.0 76 2027.0 77 1328.0 78 853.0 79 572.0 80 387.0 81 267.0 82 210.5 83 160.0 84 113.0 85 96.0 86 76.5 87 60.0 88 50.0 89 39.5 90 30.5 91 26.0 92 21.5 93 14.5 94 11.5 95 12.5 96 9.0 97 4.5 98 3.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025640732461681828 2 0.11029040440132558 3 0.017246274899904906 4 0.017999012865149374 5 0.02012383015944705 6 0.04677456546057082 7 0.04719381192222597 8 0.039466337367627696 9 0.03382556679263117 10-11 0.03743203919573283 12-13 0.03633151723388807 14-15 0.032320090862142235 16-17 0.03856114614359954 18-19 0.03743680336006982 20-21 0.049075656835337135 22-23 0.04849442878622432 24-25 0.06714136800120438 26-27 0.05941389344660611 28-29 0.051886513794161426 30-31 0.04999037638803928 32-33 0.04529767451610383 34-35 0.046260035712175865 36-37 0.04205327860261343 38-39 0.047041358663442286 40-41 0.05659827232344484 42-43 0.05735577445302629 44-45 0.05989507404464213 46-47 0.061891258901841065 48-49 0.06149106909753388 50-51 0.05573595857844959 52-53 0.06611707266875146 54-55 0.05281076167553754 56-57 0.05709850957882882 58-59 0.05517378718668473 60-61 0.05122429495131976 62-63 0.05812756907561872 64-65 0.055512042854611046 66-67 0.05338246139597638 68-69 0.05947106341864999 70-71 0.059680686649477566 72-73 0.06267258185310746 74-75 0.05303467739937608 76-77 0.05358732046246696 78-79 0.06389697208771399 80-81 0.06419235027660739 82-83 0.054549681658539004 84-85 0.060009413988729894 86-87 0.05759398266987581 88-89 0.06607419518971855 90-91 0.071262370152701 92-93 0.07548341975527441 94-95 0.07902795802199519 96-97 0.07518804156638101 98-99 0.08413990635558578 100 0.1299854597704435 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.049502E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.15326407292116 #Duplication Level Percentage of deduplicated Percentage of total 1 73.18646447305042 39.63285937172563 2 14.97401303464738 16.21783364193246 3 5.27209926327323 8.565041508680649 4 2.3804162336087975 5.156292356083423 5 1.2557470983909147 3.400140211398386 6 0.7624426788464662 2.477325583682286 7 0.4713668844636462 1.7868238758712967 8 0.3159562064791456 1.368804790795486 9 0.2293270844099242 1.1176929145011336 >10 1.0469673444100787 9.81920066191684 >50 0.05921936121221141 2.181793031088081 >100 0.04031998542469384 4.291335181687124 >500 0.003954199400708764 1.4132756544564078 >1k 0.0015978252476577266 1.3890589248226348 >5k 2.7081783713745553E-5 0.09089782173130397 >10k+ 8.124535114123665E-5 1.091624469626957 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33240 0.3167216451231155 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25537 0.2433249293474429 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15657 0.14918504204851443 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.528328673980611E-6 0.0 5 0.0 0.0 0.0 9.528328673980611E-6 0.0 6 0.0 0.0 0.0 9.528328673980611E-6 9.528328673980611E-6 7 0.0 0.0 0.0 9.528328673980611E-6 9.528328673980611E-6 8 0.0 0.0 0.0 9.528328673980611E-6 9.528328673980611E-6 9 0.0 0.0 0.0 1.9056657347961222E-5 9.528328673980611E-6 10-11 0.0 0.0 0.0 1.9056657347961222E-5 1.9056657347961222E-5 12-13 0.0 0.0 0.0 4.764164336990306E-5 8.09907937288352E-5 14-15 0.0 0.0 0.0 7.622662939184489E-5 8.57549580658255E-5 16-17 0.0 0.0 0.0 1.0481161541378673E-4 1.0957577975077704E-4 18-19 0.0 0.0 0.0 1.0957577975077704E-4 1.2386827276174796E-4 20-21 0.0 0.0 0.0 1.4768909444669949E-4 1.3816076577271886E-4 22-23 0.0 0.0 0.0 3.5731232527427294E-4 1.5245325878368977E-4 24-25 0.0 0.0 0.0 8.956628953541775E-4 1.8580240914262193E-4 26-27 0.0 0.0 0.0 0.0018723165844371902 2.0485906649058317E-4 28-29 0.0 0.0 0.0 0.005955205421237883 2.0962323082757345E-4 30-31 0.0 0.0 0.0 0.019275808907462777 2.1915155950155409E-4 32-33 0.0 4.7641643369903055E-6 0.0 0.051243351608667734 2.286798881755347E-4 34-35 0.0 9.528328673980611E-6 0.0 0.09878494752749399 2.286798881755347E-4 36-37 0.0 9.528328673980611E-6 0.0 0.17506398272704576 2.286798881755347E-4 38-39 0.0 9.528328673980611E-6 0.0 0.2857688694256895 2.286798881755347E-4 40-41 0.0 9.528328673980611E-6 0.0 0.40552566836461484 2.286798881755347E-4 42-43 0.0 9.528328673980611E-6 0.0 0.5222667512782253 2.382082168495153E-4 44-45 0.0 9.528328673980611E-6 0.0 0.6650725772795096 2.525007098604862E-4 46-47 0.0 9.528328673980611E-6 0.0 0.8338859763964243 2.572648741974765E-4 48-49 9.528328673980611E-6 9.528328673980611E-6 0.0 1.01149878704376 2.572648741974765E-4 50-51 1.9056657347961222E-5 9.528328673980611E-6 4.7641643369903055E-6 1.1936137329895513 2.572648741974765E-4 52-53 1.9056657347961222E-5 9.528328673980611E-6 9.528328673980611E-6 1.3757096222779945 2.6202903853446684E-4 54-55 1.9056657347961222E-5 9.528328673980611E-6 9.528328673980611E-6 1.57559490120076 2.7632153154543776E-4 56-57 1.9056657347961222E-5 9.528328673980611E-6 9.528328673980611E-6 1.808071828352876 2.8108569588242805E-4 58-59 1.9056657347961222E-5 2.8584986021941836E-5 9.528328673980611E-6 2.064979390225078 2.8584986021941834E-4 60-61 1.9056657347961222E-5 2.8584986021941836E-5 9.528328673980611E-6 2.329490558379117 2.8584986021941834E-4 62-63 1.9056657347961222E-5 2.8584986021941836E-5 9.528328673980611E-6 2.593968377382797 2.8584986021941834E-4 64-65 1.9056657347961222E-5 2.8584986021941836E-5 9.528328673980611E-6 2.8679030625954027 2.906140245564087E-4 66-67 1.9056657347961222E-5 2.8584986021941836E-5 9.528328673980611E-6 3.171394623354696 2.9537818889339897E-4 68-69 1.9056657347961222E-5 2.8584986021941836E-5 9.528328673980611E-6 3.5043430122096 3.144348462413602E-4 70-71 1.9056657347961222E-5 2.8584986021941836E-5 9.528328673980611E-6 3.857315183772875 3.43019832263302E-4 72-73 1.9056657347961222E-5 2.8584986021941836E-5 9.528328673980611E-6 4.204165404163117 4.4783144767708875E-4 74-75 1.9056657347961222E-5 2.8584986021941836E-5 9.528328673980611E-6 4.549581611087925 4.811805980360209E-4 76-77 1.9056657347961222E-5 3.334915035893214E-5 9.528328673980611E-6 4.9258600745877565 4.859447623730112E-4 78-79 1.9056657347961222E-5 3.8113314695922444E-5 9.528328673980611E-6 5.333286644522831 4.859447623730112E-4 80-81 1.9056657347961222E-5 3.8113314695922444E-5 9.528328673980611E-6 5.767635507126236 4.954730910469918E-4 82-83 1.9056657347961222E-5 3.8113314695922444E-5 9.528328673980611E-6 6.201731869019783 5.240580770689337E-4 84-85 1.9056657347961222E-5 3.8113314695922444E-5 9.528328673980611E-6 6.628805852680605 5.240580770689337E-4 86-87 1.9056657347961222E-5 3.8113314695922444E-5 9.528328673980611E-6 7.068833599173703 5.28822241405924E-4 88 1.9056657347961222E-5 3.8113314695922444E-5 9.528328673980611E-6 7.416670001581703 5.335864057429143E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15725 0.0 50.696846 1 GTATCAA 29305 0.0 42.40265 1 CAACGCA 35590 0.0 34.325626 5 ATCAACG 35790 0.0 34.055695 3 TCAACGC 37485 0.0 32.716007 4 TATCAAC 37805 0.0 32.53116 2 AACGCAG 38070 0.0 32.435593 6 ACGCAGA 43400 0.0 28.106228 7 GTACATG 41710 0.0 28.100224 1 CGCAGAG 44485 0.0 27.282097 8 TACATGG 43165 0.0 26.726547 2 ACATGGG 43365 0.0 25.80975 3 GCAGAGT 49015 0.0 24.138958 9 GTACTAG 3535 0.0 23.68277 1 CATGGGG 29310 0.0 23.613708 4 TACCTGG 6935 0.0 22.51483 2 GAGTACT 27500 0.0 20.974716 12-13 ATGGGGG 20000 0.0 20.697556 5 CAGAGTA 48045 0.0 20.236631 10-11 GTGGTAT 8370 0.0 20.116827 1 >>END_MODULE