FastQCFastQC Report
Mon 6 Jun 2016
ERR1211348_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211348_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10495020
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289370.2757212468389769No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234730.22365845896434688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149360.14231511707457442No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159100.052.0019951
GTATCAA285450.040.574481
CAACGCA358900.031.4102365
ATCAACG363650.031.0439823
TATCAAC376100.030.3750882
TCAACGC378300.029.9907554
AACGCAG384650.029.6121316
GTACATG410100.027.5667061
TACATGG424050.026.342942
ACGCAGA438600.025.7236867
ACATGGG424650.025.6343463
CGCAGAG449300.025.0379928
GTGGTAT79050.024.8148821
TACCTGG67650.024.6196842
CATGGGG288100.023.53384
TGGTATC83450.023.106672
GCAGAGT500900.022.223949
GTACTAG35950.022.0610221
ATGGGGG191750.021.5541745
GAGTACT283250.020.40964512-13
Produced by FastQC (version 0.11.5)